##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938420_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3306310 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13455423115195 31.0 31.0 34.0 28.0 34.0 2 31.29672142055645 31.0 31.0 34.0 28.0 34.0 3 31.357032764622797 31.0 31.0 34.0 28.0 34.0 4 35.02868575541918 37.0 35.0 37.0 32.0 37.0 5 35.02224292337984 37.0 35.0 37.0 32.0 37.0 6 34.99926413433707 37.0 35.0 37.0 32.0 37.0 7 34.91210926985068 37.0 35.0 37.0 32.0 37.0 8 34.8670300123098 37.0 35.0 37.0 32.0 37.0 9 36.35006911027702 39.0 37.0 39.0 32.0 39.0 10-11 36.19265994416736 39.0 35.0 39.0 32.0 39.0 12-13 36.017268646920584 38.0 35.0 39.0 30.0 39.0 14-15 37.19153981326615 39.0 36.5 41.0 31.0 41.0 16-17 37.124130526175705 39.0 36.0 41.0 31.0 41.0 18-19 37.11384746136932 39.5 36.0 41.0 31.0 41.0 20-21 37.03410478751236 39.0 36.0 41.0 31.0 41.0 22-23 36.88714488357111 39.0 36.0 41.0 30.0 41.0 24-25 36.7445188140253 39.0 36.0 41.0 30.0 41.0 26-27 36.53805057601979 39.0 36.0 41.0 30.0 41.0 28-29 36.31100063212463 39.0 35.5 40.5 29.5 41.0 30-31 36.03042303958189 38.5 35.0 40.0 28.5 41.0 32-33 35.72215097797847 38.0 35.0 40.0 27.0 41.0 34-35 35.42772486548448 38.0 34.0 40.0 26.5 41.0 36-37 35.218281104917565 38.0 34.0 40.0 25.5 41.0 38-39 34.976248294926975 38.0 34.0 40.0 25.0 41.0 40-41 34.692272654409294 38.0 33.5 40.0 24.5 41.0 42-43 34.26895889980068 38.0 33.0 40.0 23.5 41.0 44-45 33.809299793425296 38.0 33.0 40.0 22.5 41.0 46-47 33.32362633872807 37.5 32.0 40.0 19.5 41.0 48-49 32.81931065145132 37.0 31.0 40.0 16.0 41.0 50-51 32.07396266532781 36.0 30.5 39.0 14.0 40.5 52-53 32.0551067504257 36.0 31.0 39.0 12.5 40.0 54-55 32.02525852082836 36.0 31.0 39.0 8.5 40.0 56-57 31.59731165559188 36.0 30.0 39.0 7.5 40.0 58-59 31.14079048849019 35.5 29.5 39.0 3.5 40.0 60-61 30.593280424400618 35.0 28.5 39.0 2.0 40.0 62-63 29.875678324174082 34.5 28.0 38.0 2.0 40.0 64-65 29.213412535424688 34.0 26.5 38.0 2.0 40.0 66-67 28.522534033408846 34.0 26.0 37.0 2.0 39.5 68-69 27.70011220968391 33.0 24.5 36.5 2.0 39.0 70-71 26.877578932405008 32.5 22.5 36.0 2.0 39.0 72-73 25.989501740611136 32.0 20.0 35.0 2.0 38.0 74-75 25.072173813102822 31.0 16.0 35.0 2.0 37.5 76-77 24.14468138196358 30.5 7.5 34.5 2.0 36.5 78-79 23.179068659623567 30.0 4.0 34.0 2.0 36.0 80-81 22.25028037298378 29.0 2.0 34.0 2.0 35.5 82-83 21.258145031772578 28.5 2.0 34.0 2.0 35.0 84-85 20.283570354866907 26.5 2.0 33.0 2.0 35.0 86-87 19.313949992589926 25.5 2.0 33.0 2.0 35.0 88-89 18.22601994368344 24.0 2.0 32.0 2.0 34.0 90-91 17.084284897665373 19.5 2.0 31.5 2.0 34.0 92-93 15.935509525725053 12.0 2.0 31.0 2.0 34.0 94-95 14.674753879702749 2.0 2.0 31.0 2.0 34.0 96-97 13.246446491708278 2.0 2.0 30.0 2.0 34.0 98-99 11.52763019801531 2.0 2.0 29.0 2.0 34.0 100-101 8.110256600258294 2.0 2.0 10.5 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5004034618103255 1101 2 0.4519277542972411 1101 3 0.4863369990846884 1101 4 0.3193994669094309 1101 5 0.35010574498272007 1101 6 0.5333855241593639 1101 7 0.392965655462568 1101 8 0.3964359154531252 1101 9 0.4209501243470726 1101 10-11 0.5398839914500186 1101 12-13 0.48985495203812235 1101 14-15 0.5490540105342774 1101 16-17 0.6481585408122186 1101 18-19 0.6119967517928018 1101 20-21 0.6386593347551965 1101 22-23 0.6368472853388099 1101 24-25 0.6985964421266218 1101 26-27 0.7467985485069377 1101 28-29 0.7196165747725516 1101 30-31 0.7841565051587125 1101 32-33 0.892734278726401 1101 34-35 0.8146720939901755 1101 36-37 0.8210767753819823 1101 38-39 0.9641513008843745 1101 40-41 0.87797814871724 1101 42-43 1.0219695625911385 1101 44-45 1.1868663290450954 1101 46-47 1.2809982838910372 1101 48-49 1.0032626674583582 1101 50-51 1.1764523031110414 1101 52-53 1.226915578407521 1101 54-55 1.496613381357136 1101 56-57 1.514296745902172 1101 58-59 1.5630338224046803 1101 60-61 1.4801257157897254 1101 62-63 1.498538325078929 1101 64-65 1.4830818410787892 1101 66-67 1.7736415841778985 1101 68-69 1.7744730390294485 1101 70-71 2.123504264392757 1101 72-73 2.282195760570705 1101 74-75 2.2551464464475046 1101 76-77 2.370289088569894 1101 78-79 2.4545551529962637 1101 80-81 2.6846928551209857 1101 82-83 2.9927872634305395 1101 84-85 2.871477942674421 1101 86-87 2.9226425300806653 1101 88-89 3.3558684453289978 1101 90-91 3.3960724929347883 1101 92-93 3.4713810066346316 1101 94-95 3.4674497429732067 1101 96-97 3.289938528359391 1101 98-99 2.9157795063231298 1101 100-101 2.289955508165338 1102 1 0.5236843182077884 1102 2 0.44971490425253435 1102 3 0.37696309774114667 1102 4 0.32386599449043985 1102 5 0.3071359441709802 1102 6 0.40743611486512066 1102 7 0.40841126258324323 1102 8 0.49550121539682834 1102 9 0.50618216285919 1102 10-11 0.5342310283040916 1102 12-13 0.55985442354892 1102 14-15 0.6491715823691209 1102 16-17 0.6679454145014887 1102 18-19 0.6735750995223597 1102 20-21 0.715065490270753 1102 22-23 0.5719356564443814 1102 24-25 0.7058411671012905 1102 26-27 0.6138281148690794 1102 28-29 0.5089615747884366 1102 30-31 0.48805551926551516 1102 32-33 0.5779239813979231 1102 34-35 0.6647015464889776 1102 36-37 0.8206535303857336 1102 38-39 0.8085696951209798 1102 40-41 0.9857363587855659 1102 42-43 1.1189345716162862 1102 44-45 0.9058556943908087 1102 46-47 0.9947101288989941 1102 48-49 0.9711791203477418 1102 50-51 0.9899159736749183 1102 52-53 0.9458228918360945 1102 54-55 0.9432101717719306 1102 56-57 1.224929914519258 1102 58-59 1.240754362307424 1102 60-61 1.3419734228951157 1102 62-63 1.341231360861503 1102 64-65 1.365858741065498 1102 66-67 1.5709518576903356 1102 68-69 1.5930225675399647 1102 70-71 1.533437116778238 1102 72-73 1.729030427828917 1102 74-75 1.6341660264051967 1102 76-77 1.9512446174514864 1102 78-79 2.2906263665753066 1102 80-81 2.3747896820537022 1102 82-83 2.54545382031662 1102 84-85 2.829125884492523 1102 86-87 2.9422138119323478 1102 88-89 3.131007803166991 1102 90-91 2.974399411815554 1102 92-93 2.969811983450514 1102 94-95 2.949661431702596 1102 96-97 2.742309743707624 1102 98-99 2.592917517307539 1102 100-101 2.1256849616167823 1103 1 0.5296384128186382 1103 2 0.5244570808147593 1103 3 0.4154991294840471 1103 4 0.4652656356081053 1103 5 0.35748441935923836 1103 6 0.35919141758171236 1103 7 0.49139316290685287 1103 8 0.45565421145087726 1103 9 0.6158988254725486 1103 10-11 0.6592304805593727 1103 12-13 0.577982308794212 1103 14-15 0.6309908945332126 1103 16-17 0.6991985777272234 1103 18-19 0.8076714960194238 1103 20-21 0.7320672671546049 1103 22-23 0.8505959605084215 1103 24-25 0.8507158102456103 1103 26-27 0.9082367915835761 1103 28-29 0.9857557126053891 1103 30-31 0.8239497234691271 1103 32-33 1.1424938265176294 1103 34-35 1.0854010931754416 1103 36-37 1.1193514316470825 1103 38-39 1.068948088125623 1103 40-41 1.0890108314717324 1103 42-43 1.2511071791221369 1103 44-45 1.286290253138624 1103 46-47 1.5673289182955301 1103 48-49 1.5816363885914768 1103 50-51 1.7198058003027583 1103 52-53 1.6905939564546273 1103 54-55 1.8064939122986843 1103 56-57 1.9392021151603416 1103 58-59 2.01811864759917 1103 60-61 2.2445517668218287 1103 62-63 2.3359342312629146 1103 64-65 2.4645140277879456 1103 66-67 2.720739935259754 1103 68-69 2.6263278015693743 1103 70-71 2.685617359845434 1103 72-73 2.911649173405877 1103 74-75 3.0111166548874806 1103 76-77 3.2141480040475194 1103 78-79 3.4086428405453795 1103 80-81 3.313935737086709 1103 82-83 3.343070254410879 1103 84-85 3.4229621132044272 1103 86-87 3.5393436843954653 1103 88-89 3.941718896312075 1103 90-91 3.987661352102613 1103 92-93 3.999070862940103 1103 94-95 3.8896441667891484 1103 96-97 3.7706547659131946 1103 98-99 3.3914949147313873 1103 100-101 2.255219356698472 1104 1 0.4975563762619366 1104 2 0.4252722413798651 1104 3 0.3863909999152888 1104 4 0.2496534931260328 1104 5 0.2260022873945431 1104 6 0.3958000673950153 1104 7 0.43824600298188443 1104 8 0.35191248998597047 1104 9 0.4433952451163705 1104 10-11 0.517093280185712 1104 12-13 0.521231170775259 1104 14-15 0.47968655070857835 1104 16-17 0.529604327567057 1104 18-19 0.5346098351534252 1104 20-21 0.4415283551636193 1104 22-23 0.5627404877659146 1104 24-25 0.5674660483821228 1104 26-27 0.5988894159935967 1104 28-29 0.5986891197699791 1104 30-31 0.524934839106578 1104 32-33 0.6817631117399756 1104 34-35 0.6755517263398971 1104 36-37 0.8730355043923481 1104 38-39 0.8907624147655682 1104 40-41 0.6642474980003428 1104 42-43 0.9623333149808744 1104 44-45 0.9885419533284647 1104 46-47 1.0288325605186301 1104 48-49 1.0703328423899308 1104 50-51 1.1158619631937583 1104 52-53 1.0927578669756386 1104 54-55 1.2898097848446497 1104 56-57 1.543928338706479 1104 58-59 1.6062656106748427 1104 60-61 1.6408038586650662 1104 62-63 1.5645877668039248 1104 64-65 1.6129385943743912 1104 66-67 1.8292473012577979 1104 68-69 2.0845957016885563 1104 70-71 2.136408515023515 1104 72-73 2.278489118574406 1104 74-75 2.28052387266057 1104 76-77 2.2675575103852985 1104 78-79 2.599916792885356 1104 80-81 2.9001941741238255 1104 82-83 2.9926810296851762 1104 84-85 3.0525152244201834 1104 86-87 3.0507380369172594 1104 88-89 3.006134834252908 1104 90-91 2.913729208352528 1104 92-93 3.0812659216651017 1104 94-95 3.051816380770971 1104 96-97 2.887714543161609 1104 98-99 2.6875534807175683 1104 100-101 1.902685497342942 1105 1 0.583840781163687 1105 2 0.5446939153194279 1105 3 0.5062664347695005 1105 4 0.5413222448311714 1105 5 0.49230679348073636 1105 6 0.5499555137275394 1105 7 0.5318604136369274 1105 8 0.5866898008093671 1105 9 0.624415882049675 1105 10-11 0.633884695764273 1105 12-13 0.6891918335206313 1105 14-15 0.7266136280783542 1105 16-17 0.7837529886896419 1105 18-19 0.7561904780803346 1105 20-21 0.7066123716121027 1105 22-23 0.6260652971792595 1105 24-25 0.7514731671982204 1105 26-27 0.6441374366206389 1105 28-29 0.812510309697906 1105 30-31 0.9692210562248178 1105 32-33 0.8821170735251016 1105 34-35 0.8997459473200493 1105 36-37 0.9208661455522815 1105 38-39 1.0609958401233186 1105 40-41 1.1334734610407438 1105 42-43 1.278019024056512 1105 44-45 1.315400358719316 1105 46-47 1.2860029018005577 1105 48-49 1.2124665930136729 1105 50-51 1.0482644505386318 1105 52-53 1.21571831061528 1105 54-55 1.4429625750502026 1105 56-57 1.5189877446330797 1105 58-59 1.4484771084957977 1105 60-61 1.5732254294128865 1105 62-63 1.6478914855230435 1105 64-65 1.720702853563754 1105 66-67 1.9652250288061737 1105 68-69 2.1267225257438227 1105 70-71 2.191496503646981 1105 72-73 2.2549500944289527 1105 74-75 2.471952314847048 1105 76-77 2.5094177322333913 1105 78-79 2.769738440814951 1105 80-81 2.9655162383543185 1105 82-83 3.2120635473090537 1105 84-85 3.1444023750702215 1105 86-87 3.194460657534968 1105 88-89 3.248766166938818 1105 90-91 3.1422456080635897 1105 92-93 3.4218557543107284 1105 94-95 3.337906417679685 1105 96-97 3.201796256879467 1105 98-99 2.8154286780894306 1105 100-101 1.9758027782831444 1106 1 0.14400549311785937 1106 2 0.20440061874821325 1106 3 0.03955919729642687 1106 4 0.16781636137426403 1106 5 0.2227502158352479 1106 6 -0.07054350719126745 1106 7 0.23711460905850146 1106 8 -0.006304421300619367 1106 9 0.22730054918277176 1106 10-11 0.3023876744066456 1106 12-13 0.41806693201523615 1106 14-15 0.48415393324257394 1106 16-17 -0.02809078886642169 1106 18-19 0.33759883704641425 1106 20-21 0.2852755773405917 1106 22-23 0.33177280926425823 1106 24-25 0.34884655247321916 1106 26-27 0.3520709612163415 1106 28-29 0.46354079438104634 1106 30-31 0.34070046930047937 1106 32-33 0.46449940783683275 1106 34-35 0.29038375075587197 1106 36-37 0.40189156578442464 1106 38-39 0.5161329459896606 1106 40-41 0.5590933733029217 1106 42-43 0.19008207716149172 1106 44-45 0.563724743015932 1106 46-47 0.8710783648804323 1106 48-49 0.625255780755765 1106 50-51 0.4964077841617609 1106 52-53 0.6210797288823571 1106 54-55 0.5925757034319687 1106 56-57 1.0873495534286661 1106 58-59 1.0740045634780735 1106 60-61 1.3027307553661984 1106 62-63 1.4190118331624184 1106 64-65 1.3213718578816476 1106 66-67 1.659706847151078 1106 68-69 1.6527560118386617 1106 70-71 1.7430523388743104 1106 72-73 1.8063632418541538 1106 74-75 2.0164608354063738 1106 76-77 2.0962033789996646 1106 78-79 2.4966353675073663 1106 80-81 2.324201081846226 1106 82-83 2.624762209714408 1106 84-85 2.5401538940605306 1106 86-87 2.1847042065697586 1106 88-89 2.2167124699585834 1106 90-91 2.0325461494216626 1106 92-93 2.297566122911684 1106 94-95 2.346387096178125 1106 96-97 2.1813832074313826 1106 98-99 1.9795356580620798 1106 100-101 1.6710197010012795 1107 1 0.3635233016152277 1107 2 0.19929412918486022 1107 3 0.270400253426363 1107 4 0.21026724559497723 1107 5 0.3356485198910093 1107 6 0.3548123485542405 1107 7 0.3393242821430462 1107 8 0.33765017600448743 1107 9 0.4938787778016902 1107 10-11 0.435809791942269 1107 12-13 0.34227475290671094 1107 14-15 0.4817962213986533 1107 16-17 0.493887716889148 1107 18-19 0.5627889202690639 1107 20-21 0.6851053314359561 1107 22-23 0.6321503218580702 1107 24-25 0.6578624848129664 1107 26-27 0.6359641950843837 1107 28-29 0.6073080066937777 1107 30-31 0.7879193128176851 1107 32-33 0.814285999123463 1107 34-35 0.7424808437322739 1107 36-37 0.7996159458216354 1107 38-39 0.8975649855570751 1107 40-41 0.9396270086960357 1107 42-43 1.1381460703079895 1107 44-45 0.9474302191097834 1107 46-47 0.903476743670069 1107 48-49 0.8819884422297619 1107 50-51 1.0536372927891762 1107 52-53 1.0436934193477683 1107 54-55 1.134239130447142 1107 56-57 1.3611478557447754 1107 58-59 1.219584116715044 1107 60-61 1.330737364965298 1107 62-63 1.623958310569531 1107 64-65 1.7217875007296861 1107 66-67 1.9377592112800919 1107 68-69 2.0271718538587002 1107 70-71 2.248464864556315 1107 72-73 2.1994460445059474 1107 74-75 2.2041372188973227 1107 76-77 2.2055519126002636 1107 78-79 2.507135788040756 1107 80-81 2.6546376342037874 1107 82-83 2.8426505622708547 1107 84-85 2.7927703342356196 1107 86-87 2.7831010317467992 1107 88-89 2.848442734978054 1107 90-91 2.7134261434997597 1107 92-93 2.8300098568221532 1107 94-95 2.8230410580855025 1107 96-97 2.609671702212969 1107 98-99 2.4116409948420454 1107 100-101 1.7727600684079352 1108 1 0.2784414419408172 1108 2 0.24524054422739994 1108 3 0.1464696955609952 1108 4 0.16465773287254137 1108 5 0.16516670667678568 1108 6 0.2498977983336914 1108 7 0.09087581466913974 1108 8 0.19835344062426685 1108 9 0.2026004892258797 1108 10-11 0.2530501824717817 1108 12-13 0.1789361928028157 1108 14-15 0.1856508327037929 1108 16-17 0.332790167663795 1108 18-19 0.30836596365917757 1108 20-21 0.2521645668491672 1108 22-23 0.35991922336511806 1108 24-25 0.42305526157442586 1108 26-27 0.3674155940374533 1108 28-29 0.4402206245930884 1108 30-31 0.4904972590136296 1108 32-33 0.5687939237523736 1108 34-35 0.561074453081325 1108 36-37 0.6271669992270859 1108 38-39 0.6961523386201449 1108 40-41 0.6623697631513252 1108 42-43 0.7752793384386791 1108 44-45 0.7966774849544223 1108 46-47 0.8090132597899071 1108 48-49 1.0231232915905366 1108 50-51 1.0041105069099032 1108 52-53 1.1414130757008039 1108 54-55 1.2174858458970022 1108 56-57 1.3102234696923318 1108 58-59 1.4340940475791264 1108 60-61 1.6179531923129247 1108 62-63 1.6964020435924247 1108 64-65 1.6512076195727232 1108 66-67 1.8693700201360386 1108 68-69 2.267410595843657 1108 70-71 2.0114921942309074 1108 72-73 2.139954737311747 1108 74-75 2.3227298053304217 1108 76-77 2.5271690635351405 1108 78-79 2.7022746604310015 1108 80-81 2.64592865973313 1108 82-83 3.021044960387105 1108 84-85 2.9269747629997482 1108 86-87 2.8197887374848953 1108 88-89 3.109791987523465 1108 90-91 3.2528021383680183 1108 92-93 3.3944971185487294 1108 94-95 3.098337769707678 1108 96-97 2.9281483361727236 1108 98-99 2.7357900277575027 1108 100-101 1.8315577725885586 1109 1 0.6668962264092002 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0.3536739330908816 2316 88-89 0.2201746053319873 2316 90-91 0.043607664071288355 2316 92-93 0.030138408671552597 2316 94-95 -0.0950930470928899 2316 96-97 0.14916296589335865 2316 98-99 0.039344842556316095 2316 100-101 0.29980120253529385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10292.0 3 3869.0 4 4132.0 5 4900.0 6 6123.0 7 7706.0 8 10132.0 9 13104.0 10 16759.0 11 21010.0 12 25830.0 13 30819.0 14 35243.0 15 40334.0 16 46668.0 17 53941.0 18 54152.0 19 54214.0 20 58597.0 21 64407.0 22 70841.0 23 79570.0 24 88208.0 25 97682.0 26 108273.0 27 119839.0 28 133279.0 29 148510.0 30 164876.0 31 185244.0 32 209290.0 33 238253.0 34 269149.0 35 293152.0 36 287354.0 37 199580.0 38 50250.0 39 728.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.26349765703683 17.946098942789675 14.340641006060764 23.44976239411273 2 15.89206998478197 19.42271328688254 39.82494728147908 24.860269446856403 3 19.60445857752739 23.313162425228963 29.19726845562451 27.88511054161914 4 13.14644568721515 15.938957916389546 35.878337038043426 35.03625935835188 5 14.347727396538886 36.93569279752069 33.5575974383652 15.158982367575224 6 33.61090667434918 35.02965072786212 16.828982058253178 14.530460539535515 7 29.896660482150228 32.115098571025726 19.653509743336517 18.334731203487532 8 27.476575338196536 34.084134831406274 19.163789422978876 19.27550040741831 9 26.92370882110799 16.565896968316167 19.34336461566065 37.16702959491519 10-11 25.74276358826053 25.990356493844825 26.65095724022412 21.615922677670525 12-13 26.769621006848425 23.13103805905937 26.960482064476153 23.138858869616044 14-15 23.247139533754478 24.73681017946146 25.162633497122783 26.85341678966128 16-17 23.019442773011548 27.833865317903513 26.076122953326635 23.070568955758304 18-19 23.11168936232072 26.132683844479065 28.241283838700586 22.51434295449963 20-21 23.582301073453458 25.460746289023085 28.41122292728842 22.545729710235037 22-23 23.24947540575404 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22.995408447875086 48-49 23.217302130851976 26.393448325932024 26.987812034897086 23.401437508318914 50-51 23.299837276345723 26.509768939670646 27.074106490751493 23.116287293232137 52-53 23.732079082076343 26.104747060102852 27.074972859812046 23.088200998008762 54-55 23.112191895038062 26.348317690987383 27.24752131441985 23.291969099554706 56-57 23.15423345612506 26.61430134169509 26.93157253718197 23.299892664997884 58-59 23.150539437812323 26.027762910809127 27.11931179655272 23.702385854825827 60-61 22.657365607253315 26.331904146155182 27.51691961011169 23.493810636479814 62-63 22.90080930940242 26.254111960265043 27.63644145854161 23.208637271790934 64-65 22.691075397206493 26.29901325067698 27.331118711637654 23.67879264047888 66-67 22.641269174889022 26.495370260447142 27.174280767272315 23.68907979739152 68-69 23.28728050574477 25.90801624091507 27.395556902562827 23.40914635077733 70-71 23.063806463000347 26.0281135085289 26.9792611488397 23.928818879631052 72-73 23.039179877339137 26.213734078112928 26.9671496300074 23.779936414540533 74-75 23.105017250902474 26.61979410232692 26.7146846530188 23.5605039937518 76-77 23.26949574947949 26.1901207894621 26.850667601900653 23.689715859157754 78-79 23.26986135357855 26.503361540133323 26.6770609009469 23.549716205341227 80-81 23.52512443984864 26.405855323844584 26.446766847720955 23.622253388585822 82-83 23.504778112654954 26.452925725277467 26.52230262333003 23.519993538737552 84-85 23.360347123549335 26.56883525621006 26.47148276897273 23.599334851267873 86-87 23.66723619734979 26.43687026103137 26.213352908493086 23.682540633125758 88-89 23.483874402613502 26.464253913669 26.00894168303838 24.04293000067912 90-91 23.361516346339148 26.577099605224024 26.106342342680307 23.955041705756518 92-93 23.6555043230368 26.099520818741595 26.280210099875934 23.96476475834567 94-95 23.41958008744573 25.856207948165427 26.42902498885979 24.295186975529052 96-97 23.802124511197402 25.460207653280158 26.36195040951799 24.375717426004442 98-99 23.420499238033127 25.791429188503766 25.87910528936289 24.908966284100213 100-101 24.557706965401774 24.66919058020234 26.167822282732622 24.605280171663267 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 350.0 1 277.0 2 259.5 3 339.5 4 325.5 5 311.0 6 491.0 7 1032.5 8 1709.5 9 2039.5 10 1871.0 11 1642.0 12 1685.5 13 1871.5 14 2138.5 15 2386.5 16 2607.0 17 2845.5 18 3191.0 19 3668.5 20 4231.5 21 4954.0 22 5946.5 23 7295.5 24 8965.0 25 11306.0 26 14567.0 27 18714.0 28 22537.5 29 26366.5 30 31115.5 31 38864.0 32 49219.0 33 53714.0 34 54466.0 35 58102.0 36 64434.5 37 71586.5 38 76767.0 39 80612.5 40 85520.5 41 92034.0 42 96560.5 43 99218.5 44 103454.0 45 109768.0 46 113790.5 47 116570.5 48 120040.5 49 120669.5 50 119210.5 51 118497.0 52 119766.0 53 123456.0 54 126547.0 55 122467.5 56 114424.5 57 106586.0 58 100000.5 59 90223.0 60 75863.0 61 64443.0 62 55083.0 63 43701.0 64 32270.0 65 24100.0 66 18231.0 67 14024.5 68 10881.5 69 8423.5 70 6693.5 71 4933.5 72 3659.5 73 2917.0 74 2190.0 75 1619.5 76 1217.5 77 897.0 78 610.5 79 402.0 80 264.0 81 158.5 82 94.0 83 64.0 84 39.5 85 23.5 86 18.5 87 11.0 88 5.0 89 6.5 90 7.0 91 4.5 92 4.0 93 3.5 94 2.0 95 1.5 96 3.0 97 1.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3880761332119493 2 0.030850101775090663 3 0.03689914133883393 4 0.04560975831062423 5 0.06563207926661445 6 0.05559067359080062 7 0.03877434360359434 8 0.0409217526487232 9 0.0377762520755767 10-11 0.03895581479050664 12-13 0.031031572962002957 14-15 0.028687570131052442 16-17 0.02307708593568056 18-19 0.028475853746321427 20-21 0.03578006901954142 22-23 0.034373667320971114 24-25 0.043447226666586014 26-27 0.03756453569084569 28-29 0.03298238822131016 30-31 0.03585568201408821 32-33 0.029852010247073024 34-35 0.031954051495473805 36-37 0.03413170573842138 38-39 0.015833361058098003 40-41 0.013156661051141607 42-43 0.01427573337043411 44-45 0.011795627149299371 46-47 0.020385263329814808 48-49 0.017844666713042637 50-51 0.01205271133075846 52-53 0.009890179686720242 54-55 0.008272061603418918 56-57 0.00923990793361784 58-59 0.0041284695022547795 60-61 0.007107621487398338 62-63 0.012249305116580114 64-65 0.007636912449225874 66-67 0.0039469983153424815 68-69 0.006517840129933369 70-71 0.013958158793337587 72-73 0.01105461980274082 74-75 0.011977098336211668 76-77 0.00561048419537188 78-79 0.009391133922711422 80-81 0.01196197573730231 82-83 0.013746442408606573 84-85 0.008544268383787364 86-87 0.00238937062767859 88-89 0.01683145258611564 90-91 0.02694847125647625 92-93 0.010842903418009807 94-95 0.021685806836019613 96-97 0.019825727170168558 98-99 0.011856117544936803 100-101 0.12191839240724554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3306310.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.67121348565017 #Duplication Level Percentage of deduplicated Percentage of total 1 73.27817657256784 34.19981422657482 2 13.63361757744881 12.72594953077652 3 5.052299822825073 7.073908908737445 4 2.482873281922365 4.635148359936623 5 1.3871485221603421 3.2369952407024725 6 0.8554614943879454 2.3955255619999876 7 0.5892341747857163 1.9250191775125547 8 0.4230272605650336 1.5794556470464354 9 0.32243239980980803 1.3543480229592655 >10 1.7615622635399495 14.975596493237514 >50 0.13419184484791663 4.2898609151786475 >100 0.07207674539503058 6.17626886559269 >500 0.00411220251215278 1.2919057428635985 >1k 0.0036552911205452814 3.6619837175225314 >5k 1.3054611144804575E-4 0.4782195893590168 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8657 0.2618326775166273 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7041 0.2129564378415817 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4818 0.1457213630905753 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4795 0.14502572354074483 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4529 0.13698050092096628 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4497 0.13601265459076736 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4040 0.12219059918761399 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4039 0.12216035398979527 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3935 0.11901485341664877 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3541 0.10709824547607454 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3492 0.10561623078295744 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3485 0.10540451439822643 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.0245197818716332E-5 7 0.0 0.0 0.0 0.0 3.0245197818716332E-5 8 0.0 0.0 0.0 0.0 3.0245197818716332E-5 9 0.0 0.0 0.0 3.0245197818716332E-5 3.0245197818716332E-5 10-11 0.0 0.0 0.0 3.0245197818716332E-5 6.0490395637432665E-5 12-13 0.0 3.0245197818716332E-5 0.0 3.0245197818716332E-5 2.2683898364037248E-4 14-15 0.0 3.0245197818716332E-5 0.0 3.0245197818716332E-5 2.5708418145908884E-4 16-17 0.0 3.0245197818716332E-5 0.0 7.561299454679084E-5 2.72206780368447E-4 18-19 0.0 3.0245197818716332E-5 0.0 1.0585819236550717E-4 3.0245197818716334E-4 20-21 0.0 3.0245197818716332E-5 0.0 1.5122598909358167E-4 3.175745770965215E-4 22-23 0.0 3.0245197818716332E-5 0.0 4.385553683713868E-4 3.3269717600587965E-4 24-25 0.0 6.0490395637432665E-5 0.0 8.014977421959828E-4 3.3269717600587965E-4 26-27 0.0 9.0735593456149E-5 0.0 0.0017088536767574727 3.3269717600587965E-4 28-29 0.0 9.0735593456149E-5 0.0 0.004340185886985794 3.3269717600587965E-4 30-31 0.0 9.0735593456149E-5 0.0 0.013716197210787857 3.3269717600587965E-4 32-33 0.0 1.0585819236550717E-4 0.0 0.03277067183657915 3.4781977491523785E-4 34-35 0.0 1.361033901842235E-4 0.0 0.06210851372073399 3.62942373824596E-4 36-37 0.0 1.5122598909358167E-4 0.0 0.11160477995106327 3.62942373824596E-4 38-39 0.0 1.5122598909358167E-4 0.0 0.18493426206254102 3.62942373824596E-4 40-41 0.0 1.5122598909358167E-4 0.0 0.26683825775562486 3.62942373824596E-4 42-43 0.0 1.5122598909358167E-4 0.0 0.3472602387555916 3.62942373824596E-4 44-45 0.0 1.5122598909358167E-4 0.0 0.44832456726683223 3.9318757164331236E-4 46-47 0.0 1.5122598909358167E-4 0.0 0.57608028285309 4.2343276946202866E-4 48-49 0.0 1.6634858800293982E-4 0.0 0.7092650114478074 4.53677967280745E-4 50-51 0.0 1.81471186912298E-4 0.0 0.857360622567152 5.141683629181777E-4 52-53 0.0 1.81471186912298E-4 0.0 0.997668095248177 5.746587585556104E-4 54-55 0.0 1.81471186912298E-4 0.0 1.1506634284141535 5.746587585556104E-4 56-57 0.0 2.1171638473101433E-4 0.0 1.3302745356606005 5.746587585556104E-4 58-59 0.0 2.1171638473101433E-4 0.0 1.5225886259909083 5.746587585556104E-4 60-61 0.0 2.1171638473101433E-4 0.0 1.7311746327476856 5.746587585556104E-4 62-63 1.5122598909358166E-5 2.1171638473101433E-4 0.0 1.9365697711345882 7.561299454679083E-4 64-65 3.0245197818716332E-5 2.1171638473101433E-4 0.0 2.148316401063421 7.561299454679083E-4 66-67 3.0245197818716332E-5 2.1171638473101433E-4 0.0 2.400652086464971 7.561299454679083E-4 68-69 3.0245197818716332E-5 2.1171638473101433E-4 0.0 2.678182021649513 7.561299454679083E-4 70-71 3.0245197818716332E-5 2.1171638473101433E-4 0.0 2.970123793594672 7.561299454679083E-4 72-73 3.0245197818716332E-5 2.1171638473101433E-4 0.0 3.2733470243262124 7.712525443772665E-4 74-75 4.53677967280745E-5 2.1171638473101433E-4 0.0 3.572729114934776 8.014977421959828E-4 76-77 9.0735593456149E-5 2.1171638473101433E-4 0.0 3.893857502774997 8.317429400146992E-4 78-79 9.0735593456149E-5 2.4196158254973066E-4 0.0 4.2632118585371614 8.468655389240573E-4 80-81 9.0735593456149E-5 2.4196158254973066E-4 0.0 4.64398377647589 8.468655389240573E-4 82-83 9.0735593456149E-5 2.4196158254973066E-4 0.0 5.037761129476667 8.468655389240573E-4 84-85 9.0735593456149E-5 2.4196158254973066E-4 0.0 5.431735076263266 8.468655389240573E-4 86-87 9.0735593456149E-5 2.4196158254973066E-4 0.0 5.8335727744827315 8.771107367427737E-4 88-89 9.0735593456149E-5 2.4196158254973066E-4 3.0245197818716332E-5 6.2553571806636405 9.0735593456149E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5720 0.0 53.872974 1 GTATCAA 10495 0.0 43.13385 1 ATCAACG 13560 0.0 33.22535 3 TATCAAC 13730 0.0 33.056263 2 TCAACGC 13825 0.0 32.485355 4 CAACGCA 14105 0.0 31.70571 5 AACGCAG 14530 0.0 30.83767 6 ACGCAGA 16730 0.0 26.748842 7 CGCAGAG 16725 0.0 26.586416 8 TGGTATC 2670 0.0 25.275398 2 TACATGG 13600 0.0 24.181772 2 GCAGAGT 18460 0.0 23.442852 9 GTACATG 14300 0.0 23.350515 1 ACATGGG 13770 0.0 22.675264 3 GTGGTAT 3145 0.0 22.599566 1 AGGTAAG 1825 0.0 22.39528 2 TAAGGTG 2120 0.0 22.193216 5 GAGTACT 9920 0.0 21.644484 12-13 CAGAGTA 17150 0.0 21.244432 10-11 AGAGTAC 16215 0.0 21.15116 10-11 >>END_MODULE