FastQCFastQC Report
Thu 26 May 2016
SRR938420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3306310
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86600.26192341311008344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75090.22711119042074096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48570.14690092580550523No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48280.14602381506876244No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA46830.1416382613850486No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46090.13940011674646358No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA44450.13443990430419409No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC43460.13144562972014118No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG42040.12715081162988348No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA34530.10443666806802751No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34510.10437617767239007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62000.052.265861
GTATCAA106250.039.442561
ATCAACG136950.030.3126373
TCAACGC137250.030.2117754
TATCAAC140550.029.8421752
CAACGCA139850.029.6840575
AACGCAG145050.028.5871496
ACGCAGA166950.024.7802727
TAAGGTG19950.024.5227185
CGCAGAG168900.024.409818
TACATGG131750.023.7233412
GTACATG136450.023.5395721
AGGTAAG19750.023.3294892
GTAAGGT20650.023.00144
TGGTATC30950.022.8678972
GTGGTAT35650.022.2578071
ACATGGG133900.022.134943
AAGGTAA19600.022.0602661
GAGTACT104200.021.65178112-13
GGTAAGG21800.021.570143
Produced by FastQC (version 0.11.5)