##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938420_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3306310 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.859460546651704 31.0 31.0 34.0 30.0 34.0 2 32.11736981710729 33.0 31.0 34.0 30.0 34.0 3 32.17409680278014 34.0 31.0 34.0 30.0 34.0 4 35.816367491251576 37.0 35.0 37.0 35.0 37.0 5 35.707217714007456 37.0 35.0 37.0 33.0 37.0 6 35.73978695282656 37.0 35.0 37.0 33.0 37.0 7 35.66352671104645 37.0 35.0 37.0 33.0 37.0 8 35.63087520528928 37.0 35.0 37.0 33.0 37.0 9 37.19008290208722 39.0 37.0 39.0 33.0 39.0 10-11 37.122166403029354 39.0 37.0 39.0 33.0 39.0 12-13 37.00705922917089 39.0 37.0 39.0 33.0 39.0 14-15 38.04709918307721 40.0 37.0 41.0 33.0 41.0 16-17 38.129329373228764 40.0 37.0 41.0 33.0 41.0 18-19 38.260161176659174 40.0 38.0 41.0 33.0 41.0 20-21 38.21361880767381 40.0 38.0 41.0 33.0 41.0 22-23 38.09449098844331 40.0 37.0 41.0 33.0 41.0 24-25 37.96517809884736 40.0 37.0 41.0 33.0 41.0 26-27 37.749832441604084 40.0 37.0 41.0 32.0 41.0 28-29 37.61381736739749 40.0 37.0 41.0 32.0 41.0 30-31 37.3546131487973 39.0 36.0 41.0 31.5 41.0 32-33 37.252989737804384 39.0 36.0 41.0 31.0 41.0 34-35 37.13036300286422 39.0 36.0 41.0 31.0 41.0 36-37 36.996701761177874 39.0 36.0 41.0 31.0 41.0 38-39 36.773722518457134 39.0 35.0 41.0 30.0 41.0 40-41 36.546612689070294 39.0 35.0 40.5 30.0 41.0 42-43 36.28004754545097 39.0 35.0 40.0 30.0 41.0 44-45 35.98358275539802 38.0 35.0 40.0 29.0 41.0 46-47 35.56859625987884 38.0 34.5 40.0 27.5 41.0 48-49 35.14493090484558 38.0 34.0 40.0 26.5 41.0 50-51 34.73945637281441 38.0 33.5 40.0 26.0 41.0 52-53 34.14042799979433 37.5 33.0 40.0 24.0 41.0 54-55 33.70627194667167 37.0 32.5 40.0 23.0 41.0 56-57 33.29999576567231 36.5 32.0 39.0 22.0 40.5 58-59 32.75599853008339 36.0 31.0 39.0 20.5 40.0 60-61 32.2543649869492 35.5 31.0 39.0 18.5 40.0 62-63 31.756929477272244 35.0 30.5 38.5 16.5 40.0 64-65 31.109179115086 35.0 29.5 38.0 11.5 40.0 66-67 30.465293786729013 34.0 29.0 37.5 8.0 40.0 68-69 29.74014127531901 34.0 28.0 37.0 6.5 39.0 70-71 28.971264491230407 33.5 26.5 36.0 2.0 39.0 72-73 28.413044602593224 33.0 26.0 35.5 2.0 38.5 74-75 27.726564810922145 32.5 25.5 35.0 2.0 37.5 76-77 26.570716448245932 31.0 24.5 34.0 2.0 36.0 78-79 27.079618063641945 32.0 25.0 35.0 2.0 36.0 80-81 26.72792372766014 32.0 25.0 35.0 2.0 36.0 82-83 26.081064842679602 32.0 23.5 34.5 2.0 36.0 84-85 25.339447904159016 31.0 20.5 34.0 2.0 35.0 86-87 24.478640085170476 31.0 17.5 34.0 2.0 35.0 88-89 23.52931198224002 30.0 9.0 34.0 2.0 35.0 90-91 22.703941251727755 29.5 2.0 34.0 2.0 35.0 92-93 21.67699323414925 29.0 2.0 34.0 2.0 35.0 94-95 20.54372896068427 28.0 2.0 33.0 2.0 35.0 96-97 19.198885464460382 26.0 2.0 33.0 2.0 34.0 98-99 17.45587724684013 22.0 2.0 32.0 2.0 34.0 100-101 13.111075942667203 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18381483198801263 1101 2 0.1878569176449787 1101 3 0.22539103593278043 1101 4 0.0690807966339051 1101 5 0.09991706559866742 1101 6 0.16779043234825508 1101 7 0.19573244165455606 1101 8 0.11352994579769415 1101 9 0.16951448968628569 1101 10-11 0.20796154562692237 1101 12-13 0.25647319527544 1101 14-15 0.30500269176850736 1101 16-17 0.2588834921213703 1101 18-19 0.3525773145545088 1101 20-21 0.25801232617358494 1101 22-23 0.24975136072880133 1101 24-25 0.20402008465120502 1101 26-27 0.22850923687544622 1101 28-29 0.29622439600607464 1101 30-31 0.41798815880731865 1101 32-33 0.37045378142590124 1101 34-35 0.2422514524911108 1101 36-37 0.27477076049775206 1101 38-39 0.378855940386039 1101 40-41 0.4377632538587122 1101 42-43 0.48907481274069653 1101 44-45 0.5415869936330608 1101 46-47 0.5692746410444798 1101 48-49 0.47462987339593354 1101 50-51 0.6678080585081858 1101 52-53 0.6788392085834047 1101 54-55 0.7964477790841045 1101 56-57 0.9629243188219334 1101 58-59 0.9169337871451475 1101 60-61 0.9152752822017831 1101 62-63 1.073610781973695 1101 64-65 0.9782107749972475 1101 66-67 0.9979338201612045 1101 68-69 1.3103610993051937 1101 70-71 1.4777774481318708 1101 72-73 1.4227436999062242 1101 74-75 1.496328131178462 1101 76-77 1.4770504949113707 1101 78-79 1.581871428205936 1101 80-81 1.4981534392678846 1101 82-83 1.667263984670516 1101 84-85 1.9315897578488759 1101 86-87 1.8872089763960957 1101 88-89 1.8345944606274287 1101 90-91 2.17045077424169 1101 92-93 2.495090756051365 1101 94-95 2.4102620544947015 1101 96-97 2.199648835049988 1101 98-99 2.0689727779947376 1101 100-101 1.9762695541764224 1102 1 0.2584194744367174 1102 2 0.13739731349927098 1102 3 0.10548761109596683 1102 4 0.1971341826619195 1102 5 0.08545358647036494 1102 6 0.18527616654223067 1102 7 0.14453272581389598 1102 8 0.2112507737610798 1102 9 0.19560042157604585 1102 10-11 0.22455440526155002 1102 12-13 0.29355232621238514 1102 14-15 0.41172150194653057 1102 16-17 0.21839963190925715 1102 18-19 0.2855271189681332 1102 20-21 0.20347388550251821 1102 22-23 0.26779518405100333 1102 24-25 0.20227873542962982 1102 26-27 0.38994703596517866 1102 28-29 0.24583833805008481 1102 30-31 0.19396017065454885 1102 32-33 0.1328331305177386 1102 34-35 0.1745285105709442 1102 36-37 0.3484340457408379 1102 38-39 0.355274321467121 1102 40-41 0.33876066947849637 1102 42-43 0.3274883335357188 1102 44-45 0.19185949445754602 1102 46-47 0.19936254036569778 1102 48-49 0.2844455508805268 1102 50-51 0.41509597896897077 1102 52-53 0.3535189509347205 1102 54-55 0.32216666048341835 1102 56-57 0.49309334010076356 1102 58-59 0.6696984320797981 1102 60-61 0.4609618690552324 1102 62-63 0.5927934087872906 1102 64-65 0.7640359915984334 1102 66-67 0.7976886964380299 1102 68-69 0.9735189832979607 1102 70-71 1.1409787680393073 1102 72-73 1.2980057897203565 1102 74-75 1.2331675593758362 1102 76-77 1.1724035211584578 1102 78-79 1.2145045246944548 1102 80-81 1.3101262259301798 1102 82-83 1.4246490541475652 1102 84-85 1.6613381879184104 1102 86-87 1.5605998742243905 1102 88-89 1.3748656209492758 1102 90-91 1.6485915084197877 1102 92-93 1.829710495599187 1102 94-95 1.7127808385431287 1102 96-97 1.8575997395377009 1102 98-99 1.9482463956779412 1102 100-101 1.7243916596773836 1103 1 0.2945021575970692 1103 2 0.3033749173133202 1103 3 0.2751700506194723 1103 4 0.2275701655459912 1103 5 0.236436240490697 1103 6 0.20874878040192613 1103 7 0.23831735697356038 1103 8 0.2582987583891665 1103 9 0.3101015247339092 1103 10-11 0.32226034084239075 1103 12-13 0.40619597226412907 1103 14-15 0.5332513200733757 1103 16-17 0.4548012120432148 1103 18-19 0.38036090035664927 1103 20-21 0.4804396317389603 1103 22-23 0.5094289312602385 1103 24-25 0.41883295010612187 1103 26-27 0.5534052331839519 1103 28-29 0.5467258488132742 1103 30-31 0.6349683386237501 1103 32-33 0.5979532947535162 1103 34-35 0.6202048328814627 1103 36-37 0.6105429834009115 1103 38-39 0.5962838587981167 1103 40-41 0.7284674507771811 1103 42-43 0.7285464667779138 1103 44-45 0.789483487428889 1103 46-47 0.8552354044351844 1103 48-49 0.8792420856283556 1103 50-51 0.8850391449772488 1103 52-53 0.9232597800449369 1103 54-55 0.9940475829731525 1103 56-57 1.1487834217585444 1103 58-59 1.263924709803895 1103 60-61 1.2194527337688683 1103 62-63 1.0671794965097057 1103 64-65 1.3288787779100737 1103 66-67 1.364279883882844 1103 68-69 1.4623196852875857 1103 70-71 1.5610625948906325 1103 72-73 1.5957604198064388 1103 74-75 1.6725994276016003 1103 76-77 1.6144932658436915 1103 78-79 1.5401365673221363 1103 80-81 1.7886932490691088 1103 82-83 2.065918605895199 1103 84-85 2.2711508154059956 1103 86-87 2.239489847647821 1103 88-89 2.339140341216048 1103 90-91 2.6166284132677653 1103 92-93 2.5143038608769928 1103 94-95 2.1589754369038374 1103 96-97 2.4358284207656133 1103 98-99 2.6919703914975557 1103 100-101 2.168076875171108 1104 1 0.12537589744207978 1104 2 0.14988462607352915 1104 3 0.17441154174515106 1104 4 0.12765100157769638 1104 5 0.11423506665539662 1104 6 0.05343295489527122 1104 7 0.06886171938894847 1104 8 0.00692017426217717 1104 9 0.07986506528051507 1104 10-11 0.11648984461346146 1104 12-13 0.06205009109258697 1104 14-15 0.23098402532965423 1104 16-17 0.13048659268581986 1104 18-19 0.26331047984055544 1104 20-21 0.23150274571060692 1104 22-23 0.23825779049050766 1104 24-25 0.2792559664405232 1104 26-27 0.25773182711352405 1104 28-29 0.12827953677187054 1104 30-31 0.2100471678153255 1104 32-33 0.3733487700831901 1104 34-35 0.3200393260916954 1104 36-37 0.34483310652630905 1104 38-39 0.38392218376918663 1104 40-41 0.40319271819820557 1104 42-43 0.34502270093346254 1104 44-45 0.3703783182688838 1104 46-47 0.5596548498723948 1104 48-49 0.591082595779902 1104 50-51 0.6834499321136391 1104 52-53 0.535182813507781 1104 54-55 0.7699231660432986 1104 56-57 0.8174736479613856 1104 58-59 0.7508808625200842 1104 60-61 0.7177304303356564 1104 62-63 0.7675528965979517 1104 64-65 0.907814165838662 1104 66-67 0.8847714855764615 1104 68-69 0.9410545425923083 1104 70-71 1.0161639824991475 1104 72-73 1.1835471742525492 1104 74-75 1.044168357319247 1104 76-77 1.2306638978730646 1104 78-79 1.281508560832762 1104 80-81 1.3946694911497595 1104 82-83 1.3510000375877276 1104 84-85 1.6367923318919964 1104 86-87 1.6495594300358896 1104 88-89 1.5693315508983794 1104 90-91 1.6979517108665654 1104 92-93 1.944139156823276 1104 94-95 1.8635243938327015 1104 96-97 2.030988075762952 1104 98-99 2.0850057103499395 1104 100-101 2.1012660739229307 1105 1 0.13631082869235556 1105 2 0.17976527705787504 1105 3 0.14839619868917708 1105 4 0.12417810495743709 1105 5 0.17856903468857155 1105 6 0.11136422920010602 1105 7 0.13882119189541697 1105 8 0.1803295019468223 1105 9 0.23730540137457723 1105 10-11 0.24246829309038986 1105 12-13 0.2274115943558499 1105 14-15 0.3422722115723644 1105 16-17 0.2823677214620375 1105 18-19 0.2920421686086314 1105 20-21 0.28781744374755647 1105 22-23 0.27957109614583686 1105 24-25 0.2571532864239572 1105 26-27 0.27819262768348807 1105 28-29 0.3601721438615684 1105 30-31 0.3921391611262379 1105 32-33 0.38336133685743334 1105 34-35 0.5142775519854652 1105 36-37 0.4700179681574994 1105 38-39 0.4365778159881941 1105 40-41 0.5016711348694756 1105 42-43 0.42544001961639566 1105 44-45 0.5323511746019562 1105 46-47 0.7893004216040751 1105 48-49 0.9117538792164979 1105 50-51 0.8061167740648969 1105 52-53 0.7494174771663396 1105 54-55 0.7555988810701422 1105 56-57 0.9469510030300157 1105 58-59 1.0031179321009986 1105 60-61 0.9560497621055717 1105 62-63 1.106863717170139 1105 64-65 1.3142643560365777 1105 66-67 1.3588403922105634 1105 68-69 1.4881340044686162 1105 70-71 1.468132329494786 1105 72-73 1.4813353371710924 1105 74-75 1.4188110382687817 1105 76-77 1.3623768382579264 1105 78-79 1.489111247210463 1105 80-81 1.6648493416206946 1105 82-83 1.70214016489523 1105 84-85 1.879555552096086 1105 86-87 2.001240112401838 1105 88-89 1.855667410308829 1105 90-91 1.9942831740264104 1105 92-93 1.951180084361475 1105 94-95 1.7319416446635358 1105 96-97 1.9855765033046815 1105 98-99 2.12708800646498 1105 100-101 2.0968275571132793 1106 1 0.005574302960937416 1106 2 0.034344051350338134 1106 3 0.058456240722740915 1106 4 0.03160656441215792 1106 5 0.09111300364500607 1106 6 -0.05917280960021287 1106 7 -0.029501012065345833 1106 8 0.021945213612582393 1106 9 -0.19388830490657938 1106 10-11 0.0060588922549627 1106 12-13 0.04271258420072144 1106 14-15 0.050440605258224025 1106 16-17 -0.010894488763732113 1106 18-19 0.038317175197931874 1106 20-21 0.04202556094475085 1106 22-23 0.07975145683962381 1106 24-25 0.045479381870222824 1106 26-27 -0.15687214071103028 1106 28-29 0.09345524667268279 1106 30-31 0.1555336680598458 1106 32-33 0.0028569892953314024 1106 34-35 -0.048161885046297925 1106 36-37 -0.09886215338932658 1106 38-39 0.03611387363737606 1106 40-41 0.10890253612164003 1106 42-43 0.1806205942038943 1106 44-45 0.2746851551362539 1106 46-47 -0.04644977262520911 1106 48-49 0.11726723101368464 1106 50-51 0.28984995063483865 1106 52-53 0.28959996299760604 1106 54-55 0.2611383141000232 1106 56-57 0.35881851588778346 1106 58-59 0.4865457236553752 1106 60-61 0.5952022462394879 1106 62-63 0.4165729735238237 1106 64-65 0.5887257233831527 1106 66-67 0.6838999029597552 1106 68-69 0.568450401560213 1106 70-71 0.3669059595745914 1106 72-73 0.6987825778732706 1106 74-75 0.9463565458799472 1106 76-77 0.7600963173508504 1106 78-79 0.8795348809471584 1106 80-81 0.834901600683235 1106 82-83 1.0938594425140842 1106 84-85 1.2302319391967806 1106 86-87 1.1313603482644972 1106 88-89 1.2393471824499294 1106 90-91 1.1855346454081221 1106 92-93 1.2445344758752768 1106 94-95 1.1527162434748384 1106 96-97 1.2882470007386466 1106 98-99 1.5864749733004189 1106 100-101 1.5353477438493854 1107 1 0.06586306330519065 1107 2 0.07716842744671482 1107 3 0.07021653065468314 1107 4 0.07417318851069155 1107 5 0.09930994321281617 1107 6 -5.544668177464018E-4 1107 7 -0.056380356082961214 1107 8 0.1207591993922037 1107 9 0.1319016996413538 1107 10-11 0.15532366000656594 1107 12-13 0.23017639329950867 1107 14-15 0.29637595211040235 1107 16-17 0.1843121761490849 1107 18-19 0.136040152865462 1107 20-21 0.23489448446802896 1107 22-23 0.238961377735329 1107 24-25 0.20608071089065305 1107 26-27 0.24857050929193036 1107 28-29 0.1644207000943041 1107 30-31 0.23148609897006622 1107 32-33 0.3105782592671176 1107 34-35 0.3363486666317428 1107 36-37 0.37437872472842315 1107 38-39 0.38856230459871455 1107 40-41 0.3582548277179711 1107 42-43 0.4013754614030347 1107 44-45 0.46999921671483946 1107 46-47 0.5163240223043246 1107 48-49 0.605585757808349 1107 50-51 0.5797594835889299 1107 52-53 0.5577397923913381 1107 54-55 0.7239663310321234 1107 56-57 0.5606600758057141 1107 58-59 0.6466082054378788 1107 60-61 0.7671228731803907 1107 62-63 0.9283136036528994 1107 64-65 1.114480065137446 1107 66-67 0.9997844289996287 1107 68-69 1.1072465594933085 1107 70-71 1.0958444083264958 1107 72-73 1.3969619558672868 1107 74-75 1.1821386474534066 1107 76-77 1.2762804179579206 1107 78-79 1.450144017083879 1107 80-81 1.4726502062129718 1107 82-83 1.780447880029282 1107 84-85 2.0355669617642818 1107 86-87 2.168873870481054 1107 88-89 2.3793916801725885 1107 90-91 2.4787916259251226 1107 92-93 2.4490499021193273 1107 94-95 2.000425596854601 1107 96-97 1.9346391739536735 1107 98-99 2.02270780359132 1107 100-101 1.9203559592505286 1108 1 0.05018176168093191 1108 2 0.029240195230272548 1108 3 0.043091769225078735 1108 4 0.056469483047131064 1108 5 0.027859263600653605 1108 6 0.015761372672876917 1108 7 0.061154997212412354 1108 8 -0.01595054853491007 1108 9 -0.022431570337182904 1108 10-11 0.0625560717916116 1108 12-13 0.0757815833771005 1108 14-15 0.12933344632266852 1108 16-17 0.08933063483251402 1108 18-19 -0.03236349109114656 1108 20-21 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0.3431384366211745 2316 98-99 0.29271782465928453 2316 100-101 0.1323809866598289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 31.0 4 50.0 5 109.0 6 230.0 7 630.0 8 1544.0 9 2850.0 10 4762.0 11 7144.0 12 10257.0 13 14207.0 14 19199.0 15 25390.0 16 31957.0 17 40002.0 18 40462.0 19 38194.0 20 39482.0 21 42392.0 22 46381.0 23 52249.0 24 59062.0 25 67186.0 26 77430.0 27 90416.0 28 106846.0 29 124882.0 30 146222.0 31 171647.0 32 202580.0 33 243698.0 34 298630.0 35 368816.0 36 426875.0 37 376415.0 38 125261.0 39 2819.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.979475987552846 18.622814018116504 14.913141575812874 23.484568418517775 2 16.280656093951727 20.06783089947935 38.54660863652148 25.104904370047443 3 19.637571156921332 23.357194210115452 29.573543916383795 27.43169071657942 4 13.644939524726961 16.548569250917186 35.452543772362546 34.353947451993314 5 14.316616348556604 37.06184379024516 33.553569581447285 15.067970279750947 6 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28.21014662623543 23.23385334351676 28-29 22.74180628276606 26.41738185466315 28.09091496124745 22.74989690132334 30-31 22.829090436166 25.8887853831008 27.972437551227802 23.3096866295054 32-33 22.43516922239361 25.887381665305632 28.064392220911593 23.613056891389167 34-35 23.284239068129523 26.044169411966838 27.812161878889384 22.859429641014255 36-37 23.505790886867313 25.508121305790738 27.818638663328564 23.167449144013386 38-39 22.86294075058067 25.864788518520253 27.862274513552443 23.409996217346634 40-41 23.317073554375984 25.74094620902941 27.99734596365053 22.944634272944075 42-43 23.110164384162438 25.800532496100043 27.638695119894802 23.450607999842717 44-45 22.99280598169216 25.99590144659185 27.989284661637964 23.02200791007802 46-47 23.499588211507152 26.29622639626242 27.200467348938044 23.003718043292384 48-49 23.197900378367425 26.399551161264373 27.03364173353376 23.368906726834446 50-51 23.278881895527036 26.461447958600377 27.18160124126292 23.07806890460967 52-53 23.754835451001266 26.08710314519812 27.1036896116819 23.054371792118705 54-55 23.118219404714015 26.35593758600978 27.196754085370095 23.32908892390611 56-57 23.1303174838415 26.57084483911067 26.91740943831643 23.381428238731395 58-59 23.074726810250702 26.12580187580717 27.078510484497826 23.720960829444305 60-61 22.643324431163443 26.397781817191973 27.470140428453472 23.488753323191112 62-63 22.866171048691744 26.350432960006774 27.52840780205123 23.254988189250252 64-65 22.56725628707307 26.398569038335207 27.236603486426553 23.79757118816517 66-67 22.512009279261772 26.55128584164008 27.158626832582367 23.778078046515777 68-69 23.217723701635474 26.019206756103415 27.268055728416595 23.495013813844515 70-71 22.87702433091165 26.09434358590555 26.968173115122195 24.0604589680606 72-73 22.83585107304815 26.31012167508417 26.92975588613938 23.924271365728302 74-75 22.856492585389752 26.667553859136017 26.649436985642605 23.826516569831625 76-77 22.859381001781443 26.279432358127337 27.033671978731576 23.827514661359643 78-79 22.890836007512906 26.534701827717306 26.929416781850463 23.645045382919328 80-81 23.06009276216199 26.41967392602921 26.750723239386566 23.769510072422236 82-83 23.005085470628337 26.513633743280216 26.805033569429348 23.676247216662098 84-85 22.77662797846124 26.612907982363076 26.746840908523783 23.863623130651902 86-87 23.022000523865206 26.495102828206697 26.52460805385286 23.958288594075242 88-89 22.76944690606749 26.6458680523 26.237043713384406 24.347641328248105 90-91 22.560437737399 26.61837364078512 26.5949938518414 24.226194769974484 92-93 22.694969285815823 26.25931179660586 26.71515505294454 24.330563864633774 94-95 22.455822723190312 25.927472912018672 26.947431872303547 24.669272492487472 96-97 22.730014042849593 25.56448898151988 26.918582020010835 24.786914955619697 98-99 22.259013825079922 25.967634613814187 26.5303313966325 25.243020164473386 100-101 22.531135191015235 25.379600260955804 26.92895729283457 25.160307255194393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 367.0 1 271.5 2 250.5 3 323.5 4 288.0 5 305.0 6 571.5 7 1166.5 8 1853.0 9 2222.5 10 2012.5 11 1738.5 12 1780.0 13 1942.5 14 2196.5 15 2410.0 16 2598.0 17 2894.0 18 3219.5 19 3604.5 20 4196.0 21 4981.0 22 5950.5 23 7227.5 24 8921.5 25 11270.0 26 14540.0 27 18450.5 28 22063.0 29 26151.5 30 31047.5 31 38545.0 32 49893.0 33 54447.5 34 54413.5 35 58573.0 36 64732.0 37 71511.5 38 77001.0 39 80812.0 40 86102.5 41 93217.5 42 97037.0 43 99506.0 44 104022.5 45 111212.5 46 115801.0 47 118557.0 48 121395.0 49 121391.5 50 119864.0 51 119161.0 52 120779.5 53 125659.5 54 130411.5 55 124769.5 56 114828.0 57 106156.0 58 99575.5 59 89835.5 60 74451.0 61 62826.0 62 53634.0 63 41236.5 64 28946.5 65 21324.0 66 16056.0 67 12567.5 68 9664.0 69 7438.5 70 5918.0 71 4173.0 72 3102.5 73 2592.0 74 1996.5 75 1460.5 76 1078.0 77 752.0 78 516.0 79 323.0 80 175.5 81 89.0 82 56.5 83 36.5 84 18.5 85 14.5 86 8.0 87 3.5 88 5.5 89 4.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02468008142007253 2 0.01336837743587262 3 0.0018449570669416963 4 0.0 5 3.62942373824596E-4 6 0.0 7 0.0 8 6.049039563743267E-5 9 1.81471186912298E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014820146931171004 26-27 0.008619881378334155 28-29 3.4781977491523785E-4 30-31 0.0 32-33 3.0245197818716334E-4 34-35 0.010797535621281731 36-37 0.0011341949182018625 38-39 0.012990312463138665 40-41 0.012158569523123967 42-43 0.0055197486019157305 44-45 6.502717531024012E-4 46-47 3.0245197818716336E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.049039563743267E-5 66-67 3.7806497273395415E-4 68-69 7.712525443772665E-4 70-71 4.53677967280745E-5 72-73 1.361033901842235E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5122598909358167E-4 82-83 8.468655389240573E-4 84-85 0.0053685226128221495 86-87 0.0035084429469710945 88-89 0.0 90-91 0.0010737045225644299 92-93 6.049039563743267E-4 94-95 1.5122598909358168E-5 96-97 3.0245197818716336E-5 98-99 0.0 100-101 9.0735593456149E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3306310.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.03754473410562 #Duplication Level Percentage of deduplicated Percentage of total 1 71.11154078747988 31.315776585398215 2 14.595931261058318 12.855379516895727 3 5.433543200058013 7.178397052117505 4 2.7044389291323583 4.763874012892917 5 1.5232699656424755 3.3540534627050027 6 0.98262354608357 2.5963397020464427 7 0.6458313622060962 1.990857925268753 8 0.47878057420223247 1.686745676340127 9 0.3406501798080256 1.3501257778779348 >10 1.936153856332298 15.728684832420342 >50 0.15523957455279253 4.6990187620427575 >100 0.08249508954973439 6.590671904295638 >500 0.005232805913521651 1.492613968776862 >1k 0.004131162561889668 3.907199881712673 >5k 1.3770541872965561E-4 0.49026093920900976 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8660 0.26192341311008344 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7509 0.22711119042074096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4857 0.14690092580550523 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4828 0.14602381506876244 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4683 0.1416382613850486 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4609 0.13940011674646358 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4445 0.13443990430419409 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4346 0.13144562972014118 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4204 0.12715081162988348 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 3453 0.10443666806802751 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3451 0.10437617767239007 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0245197818716332E-5 2 0.0 0.0 0.0 0.0 3.0245197818716332E-5 3 0.0 0.0 0.0 0.0 3.0245197818716332E-5 4 0.0 0.0 0.0 0.0 3.0245197818716332E-5 5 0.0 3.0245197818716332E-5 0.0 0.0 3.0245197818716332E-5 6 0.0 3.0245197818716332E-5 0.0 0.0 6.0490395637432665E-5 7 0.0 3.0245197818716332E-5 0.0 0.0 6.0490395637432665E-5 8 0.0 3.0245197818716332E-5 0.0 0.0 6.0490395637432665E-5 9 0.0 3.0245197818716332E-5 0.0 6.0490395637432665E-5 6.0490395637432665E-5 10-11 0.0 3.0245197818716332E-5 0.0 6.0490395637432665E-5 6.0490395637432665E-5 12-13 0.0 3.0245197818716332E-5 0.0 6.0490395637432665E-5 1.5122598909358167E-4 14-15 0.0 3.0245197818716332E-5 0.0 6.0490395637432665E-5 1.81471186912298E-4 16-17 0.0 3.0245197818716332E-5 0.0 7.561299454679084E-5 1.81471186912298E-4 18-19 0.0 3.0245197818716332E-5 0.0 1.0585819236550717E-4 2.4196158254973066E-4 20-21 0.0 3.0245197818716332E-5 0.0 1.5122598909358167E-4 2.72206780368447E-4 22-23 0.0 6.0490395637432665E-5 0.0 4.385553683713868E-4 2.72206780368447E-4 24-25 0.0 1.0585819236550717E-4 0.0 8.014977421959828E-4 2.72206780368447E-4 26-27 0.0 1.6634858800293982E-4 0.0 0.0017239762756668308 3.9318757164331236E-4 28-29 0.0 1.81471186912298E-4 0.0 0.004430921480441942 4.2343276946202866E-4 30-31 0.0 1.81471186912298E-4 0.0 0.01401864918897502 4.2343276946202866E-4 32-33 0.0 1.81471186912298E-4 0.0 0.033284840199497324 4.2343276946202866E-4 34-35 0.0 1.81471186912298E-4 0.0 0.06284952106729254 4.839231650994613E-4 36-37 0.0 1.81471186912298E-4 0.0 0.11243652289107797 4.839231650994613E-4 38-39 0.0 1.81471186912298E-4 0.0 0.18582649539819315 4.990457640088195E-4 40-41 0.0 1.81471186912298E-4 0.0 0.26809343346510156 5.141683629181777E-4 42-43 0.0 1.81471186912298E-4 0.0 0.34868176305307125 5.44413560736894E-4 44-45 0.0 2.1171638473101433E-4 0.0 0.449836827157768 5.897813574649685E-4 46-47 0.0 2.4196158254973066E-4 0.0 0.5779101173211223 6.35149154193043E-4 48-49 0.0 2.72206780368447E-4 0.0 0.7112007041082052 6.502717531024012E-4 50-51 0.0 2.72206780368447E-4 0.0 0.8589938632493626 6.956395498304757E-4 52-53 0.0 2.72206780368447E-4 0.0 0.9994525619194812 6.956395498304757E-4 54-55 0.0 2.72206780368447E-4 0.0 1.1526747340690982 7.25884747649192E-4 56-57 0.0 2.72206780368447E-4 0.0 1.3319682667384485 7.561299454679083E-4 58-59 0.0 2.72206780368447E-4 0.0 1.523934537293841 7.863751432866247E-4 60-61 0.0 2.72206780368447E-4 0.0 1.7327625056331681 7.863751432866247E-4 62-63 0.0 2.72206780368447E-4 0.0 1.9378551920418836 8.16620341105341E-4 64-65 0.0 2.72206780368447E-4 0.0 2.149798415756538 8.468655389240573E-4 66-67 0.0 2.72206780368447E-4 0.0 2.403631238450115 8.619881378334154E-4 68-69 0.0 2.72206780368447E-4 0.0 2.6842159386143463 8.771107367427736E-4 70-71 0.0 2.72206780368447E-4 0.0 2.9806339998366758 8.771107367427736E-4 72-73 0.0 2.72206780368447E-4 0.0 3.289921392730869 9.527237312895644E-4 74-75 0.0 2.72206780368447E-4 0.0 3.597968732514495 0.0010434593247457134 76-77 0.0 2.72206780368447E-4 0.0 3.9283370282883334 0.0010585819236550718 78-79 0.0 2.72206780368447E-4 0.0 4.3102885089419924 0.0010888271214737882 80-81 0.0 2.72206780368447E-4 0.0 4.707181117318098 0.0011341949182018625 82-83 0.0 2.72206780368447E-4 0.0 5.119876841554483 0.0012098079127486534 84-85 0.0 2.72206780368447E-4 0.0 5.536050763540019 0.0012098079127486534 86-87 0.0 2.72206780368447E-4 0.0 5.962099742613367 0.0012249305116580115 88-89 0.0 2.72206780368447E-4 0.0 6.417652912158872 0.0012400531105673696 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6200 0.0 52.26586 1 GTATCAA 10625 0.0 39.44256 1 ATCAACG 13695 0.0 30.312637 3 TCAACGC 13725 0.0 30.211775 4 TATCAAC 14055 0.0 29.842175 2 CAACGCA 13985 0.0 29.684057 5 AACGCAG 14505 0.0 28.587149 6 ACGCAGA 16695 0.0 24.780272 7 TAAGGTG 1995 0.0 24.522718 5 CGCAGAG 16890 0.0 24.40981 8 TACATGG 13175 0.0 23.723341 2 GTACATG 13645 0.0 23.539572 1 AGGTAAG 1975 0.0 23.329489 2 GTAAGGT 2065 0.0 23.0014 4 TGGTATC 3095 0.0 22.867897 2 GTGGTAT 3565 0.0 22.257807 1 ACATGGG 13390 0.0 22.13494 3 AAGGTAA 1960 0.0 22.060266 1 GAGTACT 10420 0.0 21.651781 12-13 GGTAAGG 2180 0.0 21.57014 3 >>END_MODULE