FastQCFastQC Report
Thu 26 May 2016
SRR938419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119916
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69460.2226341991258739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56560.18128693208406893No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46150.14792064914568212No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA43240.13859347495253077No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42760.13705497199283573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38870.12458668759030693No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38310.12279176747066267No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34210.10965038802326729No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33490.10734263358372469No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33170.10631696494392798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43950.051.402221
GTATCAA81100.044.1005671
ATCAACG102250.034.3273473
TCAACGC104550.033.5721784
TATCAAC105150.033.335382
CAACGCA106000.033.1129385
AACGCAG110950.031.6958966
ACGCAGA124900.027.966917
CGCAGAG126850.027.4986388
TGGTATC21250.024.6195962
GTACATG94200.024.436651
TACATGG92050.023.9740492
GTGTAGC18600.023.7802941
TAAGGTG21850.023.7258765
GCAGAGT143200.023.6284719
ACATGGG91050.022.98373
GTGGTAT25750.022.9029621
AGAGTAC127750.021.16654610-11
TAGCCCT22050.021.1380394
GAGTACT90850.020.79153312-13
Produced by FastQC (version 0.11.5)