##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938419_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3119916 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.118794223947056 31.0 31.0 34.0 28.0 34.0 2 31.16332074325078 31.0 31.0 34.0 28.0 34.0 3 31.208254965838822 31.0 31.0 34.0 27.0 34.0 4 34.861474475594854 37.0 35.0 37.0 32.0 37.0 5 34.846075984097006 37.0 35.0 37.0 32.0 37.0 6 34.85968372225406 37.0 35.0 37.0 32.0 37.0 7 34.77160699198312 37.0 35.0 37.0 32.0 37.0 8 34.734342847692055 37.0 35.0 37.0 31.0 37.0 9 36.23773140046078 39.0 35.0 39.0 32.0 39.0 10-11 36.07227293939965 39.0 35.0 39.0 30.0 39.0 12-13 35.93567855673038 38.0 35.0 39.0 30.0 39.0 14-15 36.9094132662546 39.0 36.0 41.0 31.0 41.0 16-17 36.733519748608614 39.0 36.0 41.0 30.0 41.0 18-19 36.889487088754954 39.0 36.0 41.0 30.5 41.0 20-21 36.86559029153349 39.0 36.0 41.0 30.0 41.0 22-23 36.779429798750996 39.0 36.0 41.0 30.0 41.0 24-25 36.64482345037494 39.0 36.0 41.0 30.0 41.0 26-27 36.44108030472616 39.0 36.0 41.0 29.5 41.0 28-29 36.23792050811625 39.0 35.0 40.5 29.0 41.0 30-31 35.98249183631867 39.0 35.0 40.0 27.5 41.0 32-33 35.713941336882144 38.0 35.0 40.0 27.0 41.0 34-35 35.467424443478606 38.0 34.0 40.0 26.5 41.0 36-37 35.18766066137678 38.0 34.0 40.0 25.5 41.0 38-39 35.03924208215862 38.0 34.0 40.0 25.0 41.0 40-41 34.93196146947546 38.0 34.0 40.0 25.0 41.0 42-43 34.63849267095652 38.0 33.5 40.0 24.0 41.0 44-45 34.31345651613697 38.0 33.0 40.0 23.5 41.0 46-47 33.950763738510915 38.0 33.0 40.0 22.5 41.0 48-49 33.59812507772645 38.0 32.5 40.0 20.0 41.0 50-51 32.90967192706471 37.0 31.5 39.5 19.0 40.5 52-53 32.92983625200166 37.0 31.5 39.5 18.5 40.0 54-55 33.03509084859977 37.0 32.0 40.0 17.5 41.0 56-57 32.724319500909644 37.0 31.5 40.0 14.0 41.0 58-59 32.35387667488483 36.5 31.0 39.5 11.0 41.0 60-61 31.884415958634783 36.0 31.0 39.0 8.0 40.5 62-63 31.320875946660102 35.5 29.5 39.0 7.0 40.0 64-65 30.8059912510465 35.0 29.0 38.5 2.0 40.0 66-67 30.27362707842134 35.0 28.5 38.0 2.0 40.0 68-69 29.686660153670804 34.0 28.0 38.0 2.0 40.0 70-71 29.04365614330642 34.0 26.5 37.0 2.0 39.0 72-73 28.40323938208593 34.0 26.0 36.5 2.0 39.0 74-75 27.7276761617941 33.0 25.5 36.0 2.0 39.0 76-77 26.966822824717077 32.5 24.0 35.0 2.0 38.0 78-79 26.175895761296136 32.0 21.5 35.0 2.0 37.0 80-81 25.474108918317032 31.5 19.5 35.0 2.0 36.5 82-83 24.721468622873182 31.0 17.0 34.5 2.0 36.0 84-85 23.99819290006526 31.0 9.0 34.0 2.0 35.5 86-87 23.218236164050573 30.0 4.5 34.0 2.0 35.0 88-89 22.374883330192223 29.5 2.0 34.0 2.0 35.0 90-91 21.44817424571687 29.0 2.0 34.0 2.0 35.0 92-93 20.49216725706718 27.5 2.0 34.0 2.0 35.0 94-95 19.464003197521983 26.0 2.0 33.0 2.0 35.0 96-97 18.197695707192118 24.5 2.0 32.5 2.0 34.0 98-99 16.657659693402003 11.0 2.0 32.0 2.0 34.0 100-101 13.109394451645493 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41228222387487534 1101 2 0.26343703478869074 1101 3 0.28515883803737196 1101 4 0.22771926976118095 1101 5 0.22368542677119052 1101 6 0.3864030373893357 1101 7 0.3512844432428608 1101 8 0.3966948526356333 1101 9 0.7209157494055489 1101 10-11 0.546373828595236 1101 12-13 0.6487590676341242 1101 14-15 0.7051666767975533 1101 16-17 0.5889828300678488 1101 18-19 0.5764772329445051 1101 20-21 0.6984352730773864 1101 22-23 0.6728866258109107 1101 24-25 0.708029389894179 1101 26-27 0.6585840508703598 1101 28-29 0.5418585435289955 1101 30-31 0.6578792033500136 1101 32-33 0.7289995825802649 1101 34-35 0.6309904276603362 1101 36-37 0.6863797230105035 1101 38-39 0.7758412466686551 1101 40-41 0.7680447822824021 1101 42-43 0.8350407847163623 1101 44-45 0.9578945573441118 1101 46-47 0.989021012556826 1101 48-49 0.9030731736097195 1101 50-51 1.0137920362571151 1101 52-53 0.868724235545038 1101 54-55 0.876457343777389 1101 56-57 0.8685912562188562 1101 58-59 0.8294711003081545 1101 60-61 0.7612651065694571 1101 62-63 0.8378874102616116 1101 64-65 0.7446656302965273 1101 66-67 0.7801996465900523 1101 68-69 0.7935841027103621 1101 70-71 0.9051860050639142 1101 72-73 1.008881056956426 1101 74-75 0.9530254590561178 1101 76-77 1.056667093942849 1101 78-79 1.049982803717679 1101 80-81 1.0914319662274217 1101 82-83 1.0944492530926837 1101 84-85 1.2817280174613153 1101 86-87 1.270720121274671 1101 88-89 1.039818740063989 1101 90-91 0.970512382250007 1101 92-93 1.0287694033907862 1101 94-95 0.7412646156829226 1101 96-97 0.4285346657270921 1101 98-99 0.47721314362700085 1101 100-101 0.7190992338767472 1102 1 0.47744247869543344 1102 2 0.31662727396558665 1102 3 0.29717242000707955 1102 4 0.20591979729353938 1102 5 0.2874441863954402 1102 6 0.3198897688737574 1102 7 0.408403471360792 1102 8 0.3705092565120083 1102 9 0.5441991132799942 1102 10-11 0.6083327404111856 1102 12-13 0.5648872991942895 1102 14-15 0.6890831063070166 1102 16-17 0.7370238869735175 1102 18-19 0.7157905025324851 1102 20-21 0.5785560478698599 1102 22-23 0.7389576402628606 1102 24-25 0.6607704267369954 1102 26-27 0.6664035361442728 1102 28-29 0.5589263778625693 1102 30-31 0.7600268221486814 1102 32-33 0.6178742844860565 1102 34-35 0.5677399418399531 1102 36-37 0.6788829636749085 1102 38-39 0.5827922651599877 1102 40-41 0.755353241254312 1102 42-43 0.8662666696378167 1102 44-45 0.8746561762598546 1102 46-47 0.8625064124755895 1102 48-49 0.9170123871906171 1102 50-51 0.9157893624017817 1102 52-53 0.8152013283708044 1102 54-55 0.8587089810892863 1102 56-57 0.8930498384946475 1102 58-59 0.8626105523197296 1102 60-61 0.8110253353842758 1102 62-63 0.8187478295618114 1102 64-65 0.954864440198552 1102 66-67 0.7346508292929244 1102 68-69 0.8717146113371648 1102 70-71 0.9558671589862016 1102 72-73 1.0479443005163205 1102 74-75 1.2118086538038497 1102 76-77 1.1936715716674975 1102 78-79 1.186850550203662 1102 80-81 1.5306961510880548 1102 82-83 1.334713854849543 1102 84-85 1.266422161744945 1102 86-87 1.63052290210209 1102 88-89 1.4508339605743927 1102 90-91 1.46940724977933 1102 92-93 1.5353893624875887 1102 94-95 1.6875640919486585 1102 96-97 1.8745727440365556 1102 98-99 1.9333553700315882 1102 100-101 1.5306501861472679 1103 1 0.330238517407313 1103 2 0.1981857486665426 1103 3 0.1802516626958699 1103 4 0.15859045076805955 1103 5 0.20954388784056022 1103 6 0.27002732806717944 1103 7 0.21385063313639563 1103 8 0.2761233381986017 1103 9 0.31957269748796335 1103 10-11 0.35743369050057083 1103 12-13 0.4084688900894733 1103 14-15 0.5854602802044937 1103 16-17 0.552208586068474 1103 18-19 0.6971353796406277 1103 20-21 0.4788214010512988 1103 22-23 0.5673702865944605 1103 24-25 0.5105021439939392 1103 26-27 0.6087107026332106 1103 28-29 0.7350246054126899 1103 30-31 0.7790806909277492 1103 32-33 0.728699600585756 1103 34-35 0.767376545996278 1103 36-37 0.8872568226106594 1103 38-39 0.8020403313238162 1103 40-41 0.8901062401488957 1103 42-43 0.7051381764610909 1103 44-45 0.6641092336154557 1103 46-47 0.5131024806132913 1103 48-49 0.5481122227445567 1103 50-51 0.6828996297991452 1103 52-53 0.6567413403234141 1103 54-55 0.65361069755199 1103 56-57 0.6837294056486627 1103 58-59 0.6439142202610881 1103 60-61 0.6249890582972206 1103 62-63 0.704757284884689 1103 64-65 0.8826009437081659 1103 66-67 0.8478897786609849 1103 68-69 0.9144920701182357 1103 70-71 1.0207458816452544 1103 72-73 0.9872535489470522 1103 74-75 1.0277319279339956 1103 76-77 1.0116517235620144 1103 78-79 1.0817756380579588 1103 80-81 1.2333534587818669 1103 82-83 1.1153316798648731 1103 84-85 1.1535383403466462 1103 86-87 1.4435361450226445 1103 88-89 1.5505939969269953 1103 90-91 1.519595197432622 1103 92-93 1.552988541168716 1103 94-95 1.6923724790882737 1103 96-97 1.9388268190567715 1103 98-99 1.904204929881228 1103 100-101 1.666563942457218 1104 1 0.362505054765375 1104 2 0.2664566501899621 1104 3 0.2898204993225306 1104 4 0.17531686493659038 1104 5 0.26175367950362016 1104 6 0.3424415889588346 1104 7 0.18790809464687896 1104 8 0.33200745690851363 1104 9 0.3960884656330208 1104 10-11 0.4231371812555835 1104 12-13 0.45236382107677287 1104 14-15 0.48195711387553075 1104 16-17 0.6587647920155177 1104 18-19 0.6471639545328287 1104 20-21 0.6024063666171102 1104 22-23 0.7038190047652151 1104 24-25 0.45697689706646116 1104 26-27 0.5606406623974536 1104 28-29 0.5744337112783384 1104 30-31 0.6168864278424735 1104 32-33 0.5805106426168223 1104 34-35 0.5123752182740162 1104 36-37 0.606580665616157 1104 38-39 0.6598513791275877 1104 40-41 0.5970469082818255 1104 42-43 0.49899342009812386 1104 44-45 0.5395169309409695 1104 46-47 0.5170784315222008 1104 48-49 0.36288486473868176 1104 50-51 0.39753026323812435 1104 52-53 0.619181232027465 1104 54-55 0.6611512941033837 1104 56-57 0.8556463479620149 1104 58-59 0.8289157211645133 1104 60-61 0.8148223136990289 1104 62-63 0.9967703138809973 1104 64-65 1.0170775613645624 1104 66-67 1.0000380910105342 1104 68-69 1.0088859071778487 1104 70-71 1.0470232370534127 1104 72-73 1.1618281005521496 1104 74-75 1.2137580840625901 1104 76-77 1.1291724620065011 1104 78-79 1.303131473397304 1104 80-81 1.2822915696062687 1104 82-83 1.3387916663567445 1104 84-85 1.565210173584731 1104 86-87 1.452480230597228 1104 88-89 1.3883737566669936 1104 90-91 1.298455116961108 1104 92-93 1.5032177175768204 1104 94-95 1.598917428259604 1104 96-97 1.6742117719226286 1104 98-99 1.6933886115973387 1104 100-101 1.38123714959921 1105 1 0.3784602130581938 1105 2 0.28900844667975534 1105 3 0.3221723584778573 1105 4 0.31710658934855473 1105 5 0.25382072226739183 1105 6 0.3284747362181548 1105 7 0.3203498211601712 1105 8 0.29888903620457086 1105 9 0.3761262987774785 1105 10-11 0.5429399952808112 1105 12-13 0.4901967706746717 1105 14-15 0.7117022740545451 1105 16-17 0.6312480773104738 1105 18-19 0.5434528925079789 1105 20-21 0.5387123895227646 1105 22-23 0.6081640486544657 1105 24-25 0.6092087330261862 1105 26-27 0.5799931263842595 1105 28-29 0.4921224600098313 1105 30-31 0.6648241941125193 1105 32-33 0.743902437797189 1105 34-35 0.7138374919103754 1105 36-37 0.8320070484277196 1105 38-39 0.8625706656134966 1105 40-41 0.8862068238548346 1105 42-43 0.92311649239263 1105 44-45 0.8882926927870116 1105 46-47 0.8130415321253182 1105 48-49 0.9089492902033314 1105 50-51 1.0800952553425702 1105 52-53 0.9687693464653151 1105 54-55 0.8542347811127584 1105 56-57 0.9132779806484805 1105 58-59 0.9878753330747045 1105 60-61 1.13711370529467 1105 62-63 1.1650085551308074 1105 64-65 1.3296370897207161 1105 66-67 1.401658470401994 1105 68-69 1.390893885341562 1105 70-71 1.3435496649155674 1105 72-73 1.2970373597738813 1105 74-75 1.302847189145826 1105 76-77 1.4442863511508683 1105 78-79 1.6594255399693019 1105 80-81 1.6377689967653843 1105 82-83 1.650856846301906 1105 84-85 1.500874342463657 1105 86-87 1.5594494714869533 1105 88-89 1.6538328128492914 1105 90-91 1.636724075232415 1105 92-93 1.6879235870922251 1105 94-95 1.6450790866202283 1105 96-97 1.8995609919550205 1105 98-99 1.7126643025159858 1105 100-101 1.4454280082260862 1106 1 0.3172673757811637 1106 2 0.15078148044757356 1106 3 0.1808472152895142 1106 4 0.2126488916291649 1106 5 0.2698935241576734 1106 6 0.07806517097016297 1106 7 0.24690395549757227 1106 8 0.3283389718070353 1106 9 0.057056121120695025 1106 10-11 0.2069977496839499 1106 12-13 0.22582631798955788 1106 14-15 0.5426631835310545 1106 16-17 0.33600842025877853 1106 18-19 0.17081569829962007 1106 20-21 0.3767063762504179 1106 22-23 0.3296318891310719 1106 24-25 0.3487278452333342 1106 26-27 0.24997111406462835 1106 28-29 0.49772297720074477 1106 30-31 0.12002319816428297 1106 32-33 0.3787274605422226 1106 34-35 0.1409954377468452 1106 36-37 0.1857868417137567 1106 38-39 0.3077152633154512 1106 40-41 0.49191351675223416 1106 42-43 0.16320587370068296 1106 44-45 0.343709915754836 1106 46-47 0.25769111362831865 1106 48-49 0.4925231718301788 1106 50-51 0.34723440583638876 1106 52-53 0.3579084822662111 1106 54-55 0.49269751077185475 1106 56-57 -0.2739831385115181 1106 58-59 0.12515523404439932 1106 60-61 0.7609372080954167 1106 62-63 0.5293593753632742 1106 64-65 0.48734087960572126 1106 66-67 0.1053176173849728 1106 68-69 0.4362567406251543 1106 70-71 0.3585954121068049 1106 72-73 0.6609774064587128 1106 74-75 1.024004738273355 1106 76-77 0.9686332506926405 1106 78-79 0.9011851297119371 1106 80-81 0.9129819088293942 1106 82-83 0.7607146284669355 1106 84-85 0.8692724348581748 1106 86-87 0.8248254228054321 1106 88-89 0.881920830422029 1106 90-91 1.0071847834545338 1106 92-93 0.9497512940024215 1106 94-95 0.837427582277499 1106 96-97 0.8718866768482592 1106 98-99 0.921362735670332 1106 100-101 0.3429252910018423 1107 1 0.20342384928758506 1107 2 0.12494397389905387 1107 3 0.14338000483741808 1107 4 0.07601294433279548 1107 5 0.08438364081992944 1107 6 0.34247792589305703 1107 7 0.22533627242533072 1107 8 0.17752855125585398 1107 9 0.31648786910168525 1107 10-11 0.3963733259068647 1107 12-13 0.46092855002596167 1107 14-15 0.5711024010508581 1107 16-17 0.4983512322829 1107 18-19 0.5368151199642881 1107 20-21 0.502624577477313 1107 22-23 0.48586382788761995 1107 24-25 0.5310631745746477 1107 26-27 0.44190253814027614 1107 28-29 0.5444220531786002 1107 30-31 0.6494978800329108 1107 32-33 0.6774551228094126 1107 34-35 0.6521045459650381 1107 36-37 0.5587731832357932 1107 38-39 0.5675061706822788 1107 40-41 0.5737165147074066 1107 42-43 0.7291076543350883 1107 44-45 0.5509369480496034 1107 46-47 0.526129166966605 1107 48-49 0.46017605984530974 1107 50-51 0.5540960822953309 1107 52-53 0.7675466377520408 1107 54-55 0.7316298061059214 1107 56-57 0.6874443827394927 1107 58-59 0.7331860679551312 1107 60-61 0.8001304584714326 1107 62-63 0.8083519546449729 1107 64-65 0.8945849990807879 1107 66-67 1.1003028585677086 1107 68-69 1.194668703153532 1107 70-71 1.3786549168038995 1107 72-73 1.2210979931310888 1107 74-75 1.0924141095590052 1107 76-77 1.2328265949542576 1107 78-79 1.349658268101198 1107 80-81 1.290576390609015 1107 82-83 1.128476329899442 1107 84-85 1.2275000060563244 1107 86-87 1.3522461223283067 1107 88-89 1.2348492633022694 1107 90-91 1.0862568838445341 1107 92-93 1.2093580916958437 1107 94-95 1.3612666867590377 1107 96-97 1.5842034825595093 1107 98-99 1.873558297509966 1107 100-101 1.7446555088352245 1108 1 0.29830739082360225 1108 2 0.25107410474024405 1108 3 0.24547507327329754 1108 4 0.2217275392393674 1108 5 0.2651881115818284 1108 6 0.31621932169274203 1108 7 0.32053780633619766 1108 8 0.4719640546697832 1108 9 0.4055946416548153 1108 10-11 0.44251177190642466 1108 12-13 0.3505751141729192 1108 14-15 0.4736369519675634 1108 16-17 0.5538748930244353 1108 18-19 0.6803311462990109 1108 20-21 0.462205184567118 1108 22-23 0.4486860055896713 1108 24-25 0.6035934615447331 1108 26-27 0.7209256318971597 1108 28-29 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-2.358437964071509 2316 76-77 -2.49661674059686 2316 78-79 -2.631958941923198 2316 80-81 -2.860478388721212 2316 82-83 -3.1158263979781324 2316 84-85 -3.0305337043007405 2316 86-87 -3.040204088361424 2316 88-89 -3.407205959585287 2316 90-91 -3.3422108473597234 2316 92-93 -3.526292606906953 2316 94-95 -3.751690288179974 2316 96-97 -3.763562540949028 2316 98-99 -3.708009899429488 2316 100-101 -3.225971681229092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11652.0 3 3946.0 4 4142.0 5 4629.0 6 5553.0 7 6834.0 8 8694.0 9 11078.0 10 13954.0 11 17328.0 12 20827.0 13 24453.0 14 27744.0 15 31836.0 16 36085.0 17 40457.0 18 39855.0 19 38845.0 20 41100.0 21 44582.0 22 48976.0 23 54622.0 24 60908.0 25 67945.0 26 76100.0 27 86623.0 28 97957.0 29 111841.0 30 129313.0 31 149032.0 32 174989.0 33 208419.0 34 251049.0 35 304138.0 36 356513.0 37 343951.0 38 156598.0 39 7348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09292507277125 18.587610951925935 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28.37546238716663 22.706933186356977 44-45 22.29580524419003 26.916614230749875 28.542709975795606 22.24487054926449 46-47 22.73288988619971 27.096938611957043 27.782384997595766 22.387786504247476 48-49 22.403392066212927 27.198780625602538 27.7337314571508 22.664095851033736 50-51 22.6389895052666 27.328971746921138 27.769372303392036 22.262666444420226 52-53 22.85477626133134 26.97227746861347 27.847755352791637 22.325190917263548 54-55 22.25582020063047 27.231608499387416 28.060057072041435 22.452514227940682 56-57 22.424242229958946 27.362860720851057 27.75376102091849 22.45913602827151 58-59 22.370349425355897 26.918356280146195 27.877776864104952 22.83351743039296 60-61 22.068454871363784 27.172080718000608 28.10318084733254 22.656283563303077 62-63 22.255757896898075 27.12302538480143 28.264274793572884 22.35694192472761 64-65 21.925678626695014 27.16031000496053 28.089972280344366 22.824039088000088 66-67 21.846744652154644 27.22146046084471 28.088591557083888 22.84320332991676 68-69 22.480594062897193 26.770993112755587 28.185867444987796 22.562545379359424 70-71 22.283443890367863 26.844759198987596 27.77022882725112 23.101568083393424 72-73 22.232603025644394 27.031521270405147 27.81629077221742 22.919584931733038 74-75 22.363262056757712 27.325978998883286 27.620691015034737 22.69006792932426 76-77 22.408174885377903 27.000095859049367 27.72229280739737 22.86943644817536 78-79 22.487713830002747 27.252955989035083 27.55467714265933 22.70465303830285 80-81 22.71593592856905 27.2346693728508 27.35493472591552 22.694459972664635 82-83 22.620562941726483 27.31018402618954 27.48120904478897 22.58804398729501 84-85 22.568811103566002 27.42145255339803 27.419096598786858 22.59063974424911 86-87 22.773553454079178 27.351578778650428 27.13513383555057 22.739733931719826 88-89 22.58409934243593 27.412830475445354 26.97952597350024 23.023544208618475 90-91 22.5686486978707 27.442934299475553 27.039669641693404 22.948747360960343 92-93 22.802609586974636 26.966223687952496 27.31688533457135 22.914281390501518 94-95 22.512063011457414 26.912757338098142 27.403663461301036 23.1715161891434 96-97 22.912299637747036 26.480765512312015 27.380754257041517 23.226180592899432 98-99 22.646009730749263 26.736332474800673 27.00642801414455 23.611229780305514 100-101 23.281742753429683 26.063836185514383 27.4376817646939 23.216739296362036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 274.0 1 200.5 2 166.5 3 211.5 4 211.5 5 233.5 6 378.5 7 798.0 8 1343.0 9 1590.5 10 1474.5 11 1334.0 12 1390.0 13 1497.5 14 1714.5 15 1960.0 16 2169.0 17 2555.0 18 2983.5 19 3382.0 20 4024.0 21 4956.5 22 6043.0 23 7566.0 24 9838.5 25 12877.5 26 16944.0 27 21872.0 28 26333.0 29 30978.0 30 36294.5 31 44163.5 32 55237.0 33 60378.5 34 61928.0 35 66540.5 36 72649.0 37 79571.0 38 85328.0 39 89224.5 40 93668.0 41 99678.0 42 104383.5 43 108403.5 44 111742.5 45 116153.5 46 118637.0 47 119481.5 48 119962.0 49 117225.0 50 113735.0 51 110137.5 52 106765.0 53 105383.0 54 104978.0 55 98377.0 56 88715.0 57 80759.0 58 74743.5 59 65817.0 60 52640.5 61 43616.5 62 37608.5 63 28904.5 64 20250.0 65 14855.5 66 10971.5 67 8298.5 68 6274.0 69 4831.0 70 3794.5 71 2773.0 72 2029.5 73 1601.5 74 1248.0 75 934.5 76 653.5 77 463.5 78 328.5 79 226.5 80 148.0 81 87.5 82 55.5 83 28.5 84 14.5 85 10.5 86 8.5 87 6.5 88 6.0 89 6.5 90 4.0 91 3.5 92 2.5 93 2.5 94 2.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006698898303672278 2 0.10910550155837528 3 0.11689417279183159 4 0.11990707442123441 5 0.16067740285315374 6 0.09811161582555428 7 0.028077679014435006 8 0.029359764814180893 9 0.024007056600241803 10-11 0.05266167422456246 12-13 0.054873272229124116 14-15 0.028750774059301595 16-17 0.03182777997869174 18-19 0.0375010096425673 20-21 0.05998558935561086 22-23 0.032004066776156796 24-25 0.04956864223267549 26-27 0.058222721380960255 28-29 0.054120046821773404 30-31 0.06920058104128445 32-33 0.05075457159744044 34-35 0.06360748173989299 36-37 0.05477711579414318 38-39 0.0336387261708328 40-41 0.038174104687433895 42-43 0.03355859580834868 44-45 0.007532254073507107 46-47 0.013333692317357261 48-49 0.009086783105699 50-51 0.0082053491183737 52-53 0.00979193029555924 54-55 0.011218250747776543 56-57 0.014439491319638093 58-59 0.02043324243345013 60-61 0.025962237444854284 62-63 0.011298381110260661 64-65 0.009247043830667236 66-67 0.010208608180476654 68-69 0.012211867242579609 70-71 0.03246882287856468 72-73 0.029199504089212656 74-75 0.03009696414903478 76-77 0.02413526518021639 78-79 0.004743717459059795 80-81 0.004743717459059795 82-83 0.0063142725637485115 84-85 0.005176421416474033 86-87 0.013333692317357261 88-89 0.03873501722482272 90-91 0.03591442846538176 92-93 0.03060979846893314 94-95 0.04804616534547725 96-97 0.04413580365625228 98-99 0.026747514997198643 100-101 0.07658860046232013 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3119916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.65820278282863 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3405978462803 39.88982874232423 2 13.78347762642928 14.791932750630476 3 5.109136436679449 8.224412368934535 4 2.3707647848412305 5.088439103015994 5 1.2418259521154404 3.3317074379794773 6 0.766107441347033 2.4664769064739858 7 0.5139644666266057 1.9304886701393138 8 0.3389123507415234 1.4548342113355046 9 0.2517321136031705 1.215674351880208 >10 1.1750677633941087 11.144032375325024 >50 0.06941283752136819 2.541028060483467 >100 0.03355873319398278 3.414406950776909 >500 0.0025115294900202394 0.9453705729388199 >1k 0.002810521094366226 3.1570108485729573 >5k 1.1959664231345642E-4 0.4043566491891087 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6946 0.2226341991258739 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5656 0.18128693208406893 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4615 0.14792064914568212 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4324 0.13859347495253077 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4276 0.13705497199283573 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3887 0.12458668759030693 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3831 0.12279176747066267 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3421 0.10965038802326729 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3349 0.10734263358372469 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3317 0.10631696494392798 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.205214499364727E-5 2 0.0 0.0 0.0 0.0 3.205214499364727E-5 3 0.0 0.0 0.0 0.0 3.205214499364727E-5 4 0.0 0.0 0.0 0.0 3.205214499364727E-5 5 0.0 0.0 0.0 0.0 3.205214499364727E-5 6 0.0 0.0 0.0 0.0 6.410428998729453E-5 7 0.0 0.0 0.0 0.0 6.410428998729453E-5 8 0.0 0.0 0.0 3.205214499364727E-5 6.410428998729453E-5 9 0.0 0.0 0.0 3.205214499364727E-5 6.410428998729453E-5 10-11 0.0 0.0 0.0 4.80782174904709E-5 6.410428998729453E-5 12-13 0.0 0.0 0.0 9.615643498094179E-5 9.615643498094179E-5 14-15 0.0 0.0 0.0 1.2820857997458907E-4 9.615643498094179E-5 16-17 0.0 0.0 0.0 1.2820857997458907E-4 9.615643498094179E-5 18-19 3.205214499364727E-5 0.0 0.0 1.9231286996188358E-4 9.615643498094179E-5 20-21 6.410428998729453E-5 0.0 0.0 2.5641715994917814E-4 9.615643498094179E-5 22-23 6.410428998729453E-5 0.0 0.0 5.609125373888272E-4 9.615643498094179E-5 24-25 6.410428998729453E-5 0.0 0.0 8.013036248411816E-4 9.615643498094179E-5 26-27 6.410428998729453E-5 0.0 0.0 0.001474398669707774 1.1218250747776544E-4 28-29 9.615643498094179E-5 0.0 0.0 0.004551404589097911 1.2820857997458907E-4 30-31 9.615643498094179E-5 0.0 0.0 0.013606135549803264 1.442346524714127E-4 32-33 9.615643498094179E-5 0.0 0.0 0.03088224170137914 1.6026072496823634E-4 34-35 9.615643498094179E-5 0.0 0.0 0.05819066923596661 1.6026072496823634E-4 36-37 9.615643498094179E-5 0.0 0.0 0.10434575802681867 1.6026072496823634E-4 38-39 9.615643498094179E-5 0.0 0.0 0.1773445182498503 2.2436501495553084E-4 40-41 9.615643498094179E-5 0.0 0.0 0.2532119454498134 2.2436501495553084E-4 42-43 9.615643498094179E-5 0.0 0.0 0.33651547028830264 2.2436501495553084E-4 44-45 9.615643498094179E-5 0.0 0.0 0.4391464385579612 2.403910874523545E-4 46-47 9.615643498094179E-5 0.0 0.0 0.5581240007743797 2.5641715994917814E-4 48-49 9.615643498094179E-5 0.0 0.0 0.6836081484245089 2.5641715994917814E-4 50-51 9.615643498094179E-5 0.0 0.0 0.823627943829257 2.5641715994917814E-4 52-53 9.615643498094179E-5 0.0 0.0 0.9592085171523849 2.5641715994917814E-4 54-55 9.615643498094179E-5 0.0 0.0 1.108940112490208 2.5641715994917814E-4 56-57 1.2820857997458907E-4 0.0 0.0 1.2787844288115449 2.7244323244600177E-4 58-59 1.2820857997458907E-4 0.0 0.0 1.4651035476596164 2.884693049428254E-4 60-61 1.2820857997458907E-4 0.0 0.0 1.6649005934775167 2.884693049428254E-4 62-63 1.2820857997458907E-4 0.0 0.0 1.8654348386302708 3.2052144993647267E-4 64-65 1.2820857997458907E-4 0.0 0.0 2.075584727281119 3.8462573992376715E-4 66-67 1.2820857997458907E-4 0.0 0.0 2.3211041579324574 3.8462573992376715E-4 68-69 1.2820857997458907E-4 0.0 0.0 2.5883389168169915 3.8462573992376715E-4 70-71 1.2820857997458907E-4 3.205214499364727E-5 0.0 2.8698529062961953 3.8462573992376715E-4 72-73 1.2820857997458907E-4 3.205214499364727E-5 0.0 3.165838439240031 4.3270395741423805E-4 74-75 1.2820857997458907E-4 3.205214499364727E-5 0.0 3.461359216081459 5.288603923951799E-4 76-77 1.2820857997458907E-4 3.205214499364727E-5 0.0 3.784653176559882 5.448864648920035E-4 78-79 1.442346524714127E-4 3.205214499364727E-5 0.0 4.14868541332523 5.609125373888272E-4 80-81 1.6026072496823634E-4 3.205214499364727E-5 0.0 4.531291868114398 5.769386098856508E-4 82-83 1.6026072496823634E-4 3.205214499364727E-5 0.0 4.92250432383436 5.769386098856508E-4 84-85 1.6026072496823634E-4 3.205214499364727E-5 0.0 5.325960698941895 6.089907548792981E-4 86-87 1.6026072496823634E-4 3.205214499364727E-5 0.0 5.735539033743216 6.089907548792981E-4 88-89 1.6026072496823634E-4 3.205214499364727E-5 0.0 6.163210804393452 6.089907548792981E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4395 0.0 51.40222 1 GTATCAA 8110 0.0 44.100567 1 ATCAACG 10225 0.0 34.327347 3 TCAACGC 10455 0.0 33.572178 4 TATCAAC 10515 0.0 33.33538 2 CAACGCA 10600 0.0 33.112938 5 AACGCAG 11095 0.0 31.695896 6 ACGCAGA 12490 0.0 27.96691 7 CGCAGAG 12685 0.0 27.498638 8 TGGTATC 2125 0.0 24.619596 2 GTACATG 9420 0.0 24.43665 1 TACATGG 9205 0.0 23.974049 2 GTGTAGC 1860 0.0 23.780294 1 TAAGGTG 2185 0.0 23.725876 5 GCAGAGT 14320 0.0 23.628471 9 ACATGGG 9105 0.0 22.9837 3 GTGGTAT 2575 0.0 22.902962 1 AGAGTAC 12775 0.0 21.166546 10-11 TAGCCCT 2205 0.0 21.138039 4 GAGTACT 9085 0.0 20.791533 12-13 >>END_MODULE