FastQCFastQC Report
Thu 26 May 2016
SRR938419_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938419_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119916
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72300.2317370083040697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59760.19154361848203605No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44870.14381797458649528No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44800.14359360957153974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41720.13372154891349639No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39800.1275675370747161No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39420.1263495555649575No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38450.12324049750057373No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37950.12163789025089136No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.057.2366331
GTATCAA76900.040.7318651
ATCAACG97800.031.6704393
TCAACGC99900.030.9571424
TATCAAC101750.030.5038012
CAACGCA102750.030.1909455
AACGCAG108200.028.6578316
ACGCAGA124800.024.7318257
CGCAGAG124750.024.6275448
TAAGGTG22900.023.8565565
GTACATG92750.023.7782171
TACATGG91000.023.5042462
GCGTATC1050.002188796222.6337181
GGTAAGG22350.022.1055433
GTAAGGT22350.022.1055434
ACATGGG90550.022.0871163
GAGTACT92000.021.16183912-13
AGGTAAG21250.021.0253942
GCAGAGT145350.020.973819
GTACTTT96300.020.06898914-15
Produced by FastQC (version 0.11.5)