##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938419_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3119916 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81317189308943 31.0 31.0 34.0 30.0 34.0 2 32.035070816009146 31.0 31.0 34.0 30.0 34.0 3 32.10054277102332 33.0 31.0 34.0 30.0 34.0 4 35.76669852649879 37.0 35.0 37.0 35.0 37.0 5 35.66029437972048 37.0 35.0 37.0 33.0 37.0 6 35.66178576602704 37.0 35.0 37.0 33.0 37.0 7 35.606180743327705 37.0 35.0 37.0 33.0 37.0 8 35.57911270688057 37.0 35.0 37.0 33.0 37.0 9 37.17482970695365 39.0 37.0 39.0 33.0 39.0 10-11 37.07595733346667 39.0 37.0 39.0 33.0 39.0 12-13 36.97073222484195 39.0 37.0 39.0 33.0 39.0 14-15 38.0331641941642 40.0 37.0 41.0 33.0 41.0 16-17 37.93626062368346 40.0 37.0 41.0 33.0 41.0 18-19 38.17636356235232 40.0 37.5 41.0 33.0 41.0 20-21 38.1446723245113 40.0 37.0 41.0 33.0 41.0 22-23 38.04338305903107 40.0 37.0 41.0 33.0 41.0 24-25 37.91762438475908 40.0 37.0 41.0 32.5 41.0 26-27 37.73061438192566 40.0 37.0 41.0 32.0 41.0 28-29 37.61941315086688 40.0 37.0 41.0 32.0 41.0 30-31 37.37201306060804 39.0 36.0 41.0 31.5 41.0 32-33 37.17621548144245 39.0 36.0 41.0 31.0 41.0 34-35 37.08117398032511 39.0 36.0 41.0 31.0 41.0 36-37 37.038863225804796 39.0 36.0 41.0 31.0 41.0 38-39 36.87383298140078 39.0 36.0 41.0 30.0 41.0 40-41 36.70593631366998 39.0 35.0 41.0 30.0 41.0 42-43 36.50321499040359 39.0 35.0 40.5 30.0 41.0 44-45 36.22534869528539 39.0 35.0 40.0 30.0 41.0 46-47 35.874853842218826 38.0 35.0 40.0 28.5 41.0 48-49 35.532624596303236 38.0 34.5 40.0 27.5 41.0 50-51 35.16920535681089 38.0 34.0 40.0 26.5 41.0 52-53 34.68849065167139 38.0 33.0 40.0 26.0 41.0 54-55 34.357648411046966 38.0 33.0 40.0 24.5 41.0 56-57 34.02552632827294 37.0 33.0 40.0 24.0 41.0 58-59 33.55245269423921 37.0 32.0 39.5 23.0 41.0 60-61 33.15665533943863 36.0 32.0 39.0 22.0 40.5 62-63 32.68273120173748 36.0 31.0 39.0 20.5 40.0 64-65 32.158339198875865 35.0 31.0 38.5 19.0 40.0 66-67 31.664384233421668 35.0 30.0 38.0 18.0 40.0 68-69 31.164765653947093 34.5 29.5 38.0 14.5 40.0 70-71 30.58416829171042 34.0 29.0 37.0 10.0 39.0 72-73 30.076365197011715 34.0 29.0 36.5 8.0 39.0 74-75 29.487484759205053 34.0 28.0 36.0 7.0 39.0 76-77 28.254378803788306 31.5 26.5 34.5 6.0 37.0 78-79 28.943743196932225 33.0 28.0 35.0 2.0 37.0 80-81 28.559540545322374 33.0 27.0 35.0 2.0 37.0 82-83 28.103170085348452 33.0 26.5 35.0 2.0 36.0 84-85 27.53619648734133 33.0 26.0 35.0 2.0 36.0 86-87 26.913569307635207 32.0 25.5 35.0 2.0 35.5 88-89 26.144917363159777 32.0 24.0 34.0 2.0 35.0 90-91 25.519004037288184 31.0 21.5 34.0 2.0 35.0 92-93 24.689368880444217 31.0 18.5 34.0 2.0 35.0 94-95 23.8617526882134 31.0 11.5 34.0 2.0 35.0 96-97 22.78181159364547 30.0 2.0 34.0 2.0 35.0 98-99 21.398218894354848 29.5 2.0 34.0 2.0 35.0 100-101 17.407997202488787 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24376319913346478 1101 2 0.3039992088822814 1101 3 0.27408211301979435 1101 4 0.08500291131269222 1101 5 0.05628088492756689 1101 6 0.19153082513009423 1101 7 0.230345028240464 1101 8 0.21901142358947823 1101 9 0.27516571865453443 1101 10-11 0.30390398287573817 1101 12-13 0.35590835248791564 1101 14-15 0.430451881273207 1101 16-17 0.5153606312938876 1101 18-19 0.4392672912430342 1101 20-21 0.3872173408643036 1101 22-23 0.30468659728598624 1101 24-25 0.44871632849012855 1101 26-27 0.42235382713083425 1101 28-29 0.39105143793996433 1101 30-31 0.4935803679164721 1101 32-33 0.4058845902682009 1101 34-35 0.44828103119249363 1101 36-37 0.49676216121478234 1101 38-39 0.5004458321875589 1101 40-41 0.5698191566164041 1101 42-43 0.6430592881704911 1101 44-45 0.6810789244035931 1101 46-47 0.4651744502740769 1101 48-49 0.5158892289914903 1101 50-51 0.7012974069268694 1101 52-53 0.5963002627932497 1101 54-55 0.6696795943556495 1101 56-57 0.6937611665361416 1101 58-59 0.7834519658285757 1101 60-61 0.7314923699230107 1101 62-63 0.8723678148574407 1101 64-65 0.6880969948422546 1101 66-67 0.6646932916342756 1101 68-69 0.7408399461584949 1101 70-71 0.7643028034864692 1101 72-73 0.6923371868199091 1101 74-75 0.82570273859411 1101 76-77 0.692200219483535 1101 78-79 0.8289871176419545 1101 80-81 0.8220891405579387 1101 82-83 1.083927509291513 1101 84-85 1.090570672363274 1101 86-87 1.1027571367961393 1101 88-89 1.0297485937216742 1101 90-91 1.1461678591841107 1101 92-93 1.440497948675084 1101 94-95 1.2981241696635273 1101 96-97 1.398545615825352 1101 98-99 1.7015728935008774 1101 100-101 1.7399576782214474 1102 1 0.24118854097273257 1102 2 0.25860063955574475 1102 3 0.15310334076801269 1102 4 0.20311434905953973 1102 5 0.14533939204585522 1102 6 0.15262922381067767 1102 7 0.15866568712417006 1102 8 0.24978019721303468 1102 9 0.16378963491424514 1102 10-11 0.320326477041192 1102 12-13 0.3346720027115282 1102 14-15 0.4492074080526507 1102 16-17 0.4457578400105433 1102 18-19 0.4301572373414331 1102 20-21 0.4294704214503895 1102 22-23 0.28799343335639804 1102 24-25 0.4179484485347942 1102 26-27 0.35350908992068497 1102 28-29 0.3861954682138773 1102 30-31 0.3555933019767181 1102 32-33 0.2843519671330128 1102 34-35 0.3070786586820873 1102 36-37 0.31366451053450106 1102 38-39 0.4226873129600719 1102 40-41 0.3906138778969179 1102 42-43 0.3500620053687342 1102 44-45 0.4093974161418572 1102 46-47 0.4749909489087756 1102 48-49 0.32943931708484087 1102 50-51 0.4226162422805544 1102 52-53 0.31077002485854877 1102 54-55 0.33747049565133835 1102 56-57 0.3452699912864645 1102 58-59 0.49694260264315204 1102 60-61 0.4591371475269739 1102 62-63 0.6656272775751617 1102 64-65 0.619577999700688 1102 66-67 0.5300844032994583 1102 68-69 0.5838147308626453 1102 70-71 0.5904177551367837 1102 72-73 0.704569270671012 1102 74-75 0.7762088229108137 1102 76-77 0.7860478946279876 1102 78-79 0.9534774474814931 1102 80-81 0.8258952735348757 1102 82-83 0.8649812513708213 1102 84-85 0.9532296168427941 1102 86-87 0.850009777250623 1102 88-89 0.9136133915493474 1102 90-91 1.1224616338465623 1102 92-93 1.370606055720451 1102 94-95 1.3354134817936867 1102 96-97 1.154307653424656 1102 98-99 1.4929401254573769 1102 100-101 1.7207972080268448 1103 1 0.16468076070808735 1103 2 0.20720961859700537 1103 3 0.13986497731227843 1103 4 0.06925161139071889 1103 5 0.0028663472678331914 1103 6 0.0091321344929014 1103 7 0.15932190892714004 1103 8 0.12777111687404386 1103 9 0.16550570701010514 1103 10-11 0.18210975979076238 1103 12-13 0.2512628262829111 1103 14-15 0.29355133598282634 1103 16-17 0.2777559442586721 1103 18-19 0.25918313857223296 1103 20-21 0.2777578648284731 1103 22-23 0.2326312288677883 1103 24-25 0.309622048497296 1103 26-27 0.2651531859426015 1103 28-29 0.21936962869916243 1103 30-31 0.1423381423676915 1103 32-33 0.21677381619418412 1103 34-35 0.2501403500417041 1103 36-37 0.18744272058537348 1103 38-39 0.19915394915929596 1103 40-41 0.08476634269986505 1103 42-43 0.2998249061815912 1103 44-45 0.19734760838620247 1103 46-47 0.22582978005274867 1103 48-49 0.20325268187514922 1103 50-51 0.4577859550402934 1103 52-53 0.36015331958677166 1103 54-55 0.377784518616501 1103 56-57 0.3731284947622271 1103 58-59 0.44145507801855643 1103 60-61 0.41572983299752053 1103 62-63 0.48616591328482883 1103 64-65 0.5205311488492299 1103 66-67 0.45164441439273517 1103 68-69 0.37661985772568585 1103 70-71 0.3597102204345006 1103 72-73 0.4843384993369675 1103 74-75 0.6529960182526828 1103 76-77 0.6080903719694568 1103 78-79 0.7429184610837716 1103 80-81 0.7393478855971196 1103 82-83 0.7784175880907256 1103 84-85 0.7472830624629729 1103 86-87 0.8565690657186984 1103 88-89 0.8094404588758799 1103 90-91 1.0451176264927113 1103 92-93 1.101242955436998 1103 94-95 0.8499141608009673 1103 96-97 0.9436744514658848 1103 98-99 1.2049368755970207 1103 100-101 1.4706155752063808 1104 1 0.25654615276137704 1104 2 0.2626290345146671 1104 3 0.2396866007125169 1104 4 0.12509676433855077 1104 5 0.07878896992339435 1104 6 0.003471996756154283 1104 7 0.2003528564012953 1104 8 0.15784691249717753 1104 9 0.26220573991452767 1104 10-11 0.26782938525762745 1104 12-13 0.21857560301198475 1104 14-15 0.28580580838640657 1104 16-17 0.44486855242474377 1104 18-19 0.3572556322078313 1104 20-21 0.2320156680831218 1104 22-23 0.25718326692146576 1104 24-25 0.3061209026152554 1104 26-27 0.22275122091364352 1104 28-29 0.30240855405231315 1104 30-31 0.3125846080287502 1104 32-33 0.47369168214041935 1104 34-35 0.33913454269998056 1104 36-37 0.3901743648250786 1104 38-39 0.35763347625925945 1104 40-41 0.3869523219527622 1104 42-43 0.5761405057636466 1104 44-45 0.5239261547035028 1104 46-47 0.4425602351151241 1104 48-49 0.5057965688686679 1104 50-51 0.6408167986057549 1104 52-53 0.6134177845435644 1104 54-55 0.5406902676539005 1104 56-57 0.4809655197407281 1104 58-59 0.6050614891852817 1104 60-61 0.592719540263623 1104 62-63 0.5635143941973766 1104 64-65 0.3946724589070101 1104 66-67 0.5478544889183645 1104 68-69 0.5877074931677875 1104 70-71 0.591601964888369 1104 72-73 0.49131625710269944 1104 74-75 0.5540547324402603 1104 76-77 0.688246552516592 1104 78-79 0.7582305624063004 1104 80-81 0.6325694304885623 1104 82-83 0.7165991281330975 1104 84-85 0.8404963812490855 1104 86-87 0.8885606779776118 1104 88-89 0.8058000464737987 1104 90-91 0.962386819295304 1104 92-93 1.0745882094071781 1104 94-95 1.2459304167396397 1104 96-97 1.4984942276003643 1104 98-99 1.7429883854481432 1104 100-101 1.8836396647064042 1105 1 0.17701704836403565 1105 2 0.2361980546720801 1105 3 0.18613797023493106 1105 4 0.13599191493067764 1105 5 0.11461744096240523 1105 6 0.15564518120948634 1105 7 0.17734042150251383 1105 8 0.13737266537986415 1105 9 0.187753011080126 1105 10-11 0.22713985398644354 1105 12-13 0.23177258166049342 1105 14-15 0.38493757123634964 1105 16-17 0.31425184255211747 1105 18-19 0.3180725288299655 1105 20-21 0.31542980149835387 1105 22-23 0.25584051765061133 1105 24-25 0.36112744196594093 1105 26-27 0.1972461944210906 1105 28-29 0.26245343317174985 1105 30-31 0.405572771726078 1105 32-33 0.36716476324239267 1105 34-35 0.49681181054523194 1105 36-37 0.4281063010184809 1105 38-39 0.24025778237881212 1105 40-41 0.1787694820195398 1105 42-43 0.3177283988456523 1105 44-45 0.31111627599715064 1105 46-47 0.20032542176330992 1105 48-49 0.3999212467725286 1105 50-51 0.36389049607466006 1105 52-53 0.4777315190506002 1105 54-55 0.40537773501559826 1105 56-57 0.28697556493160903 1105 58-59 0.3323582899696902 1105 60-61 0.40438136931766877 1105 62-63 0.3226176535843521 1105 64-65 0.49020952743846635 1105 66-67 0.35850462820107154 1105 68-69 0.386980799055074 1105 70-71 0.46262730233913274 1105 72-73 0.5050103121392446 1105 74-75 0.5084562064831708 1105 76-77 0.44127791957603435 1105 78-79 0.7409231069229882 1105 80-81 0.66740102077447 1105 82-83 0.48252544781147577 1105 84-85 0.7364404249840142 1105 86-87 0.6678137500694739 1105 88-89 0.6600744357576787 1105 90-91 0.9491183286772049 1105 92-93 1.1183176813799385 1105 94-95 1.2150849854776489 1105 96-97 1.335597560253003 1105 98-99 1.4078996638405386 1105 100-101 1.3024837117149062 1106 1 0.04523457245946361 1106 2 0.10037773134268946 1106 3 0.03157749938085885 1106 4 0.0766826718057132 1106 5 0.03436159849529474 1106 6 0.010787163184360793 1106 7 0.08050216468645033 1106 8 0.14826137846921483 1106 9 -0.08871977085871663 1106 10-11 0.08482757831934862 1106 12-13 0.055859136127352826 1106 14-15 0.16050040687782285 1106 16-17 0.13031847728100843 1106 18-19 0.12145385389195695 1106 20-21 0.20567467647123294 1106 22-23 0.31771792917504627 1106 24-25 0.25726558096796026 1106 26-27 0.3326663289077416 1106 28-29 0.22673686894955836 1106 30-31 0.26237819565753284 1106 32-33 0.18119468808809103 1106 34-35 0.23514475915595767 1106 36-37 0.2609558795055662 1106 38-39 0.13122367391643053 1106 40-41 0.18130937620728815 1106 42-43 0.28839465333714287 1106 44-45 0.2037478810946709 1106 46-47 -0.47023665796590564 1106 48-49 -0.19947541538186897 1106 50-51 0.16665278506204118 1106 52-53 0.06923600290559051 1106 54-55 0.14046156587839675 1106 56-57 0.13035566816100186 1106 58-59 0.009483998371599967 1106 60-61 0.18911838918288737 1106 62-63 0.0515720703352045 1106 64-65 0.23361317801135328 1106 66-67 0.0512302583438391 1106 68-69 0.10308678568683405 1106 70-71 -0.07078179621520064 1106 72-73 0.20535509319341827 1106 74-75 0.29783005207368873 1106 76-77 0.08287787843584837 1106 78-79 0.45586840091485925 1106 80-81 -0.10204627203500749 1106 82-83 0.33986121862351837 1106 84-85 0.3662923280462884 1106 86-87 0.7766522589851768 1106 88-89 0.8551852494015293 1106 90-91 0.885634410275518 1106 92-93 0.9669779090661272 1106 94-95 1.1243442436628612 1106 96-97 1.1589739245199695 1106 98-99 1.3654378393428708 1106 100-101 1.1248428326759488 1107 1 0.12505414310180996 1107 2 0.17154142465221867 1107 3 0.16993634143334901 1107 4 0.10364661785528284 1107 5 0.04390891965729793 1107 6 0.10336109340482835 1107 7 0.1023782619744722 1107 8 0.060325772728674565 1107 9 0.05736914256561221 1107 10-11 0.0968733832288251 1107 12-13 0.18054691954453972 1107 14-15 0.25107034883774304 1107 16-17 0.2590645599040968 1107 18-19 0.12377001179253 1107 20-21 0.22276716130401297 1107 22-23 0.16137999356931232 1107 24-25 0.16859715112961027 1107 26-27 0.15109724693997606 1107 28-29 0.2317044502498007 1107 30-31 0.15570973577578684 1107 32-33 0.13864338429867473 1107 34-35 0.19698218496286302 1107 36-37 0.3984116828565263 1107 38-39 0.33132004749101185 1107 40-41 0.35681480936731447 1107 42-43 0.30699813311324675 1107 44-45 0.315518507292893 1107 46-47 0.4366675956153614 1107 48-49 0.5649848926004708 1107 50-51 0.5683414585147517 1107 52-53 0.563697503235133 1107 54-55 0.5151817398296501 1107 56-57 0.4743285408539961 1107 58-59 0.4430171202745541 1107 60-61 0.5804944329561152 1107 62-63 0.81685814916532 1107 64-65 0.7351302285762671 1107 66-67 0.6568808104850952 1107 68-69 0.6363762744421528 1107 70-71 0.8026599374388468 1107 72-73 0.7947389313496522 1107 74-75 0.7056333072754164 1107 76-77 0.7811909418668428 1107 78-79 1.0524461766897453 1107 80-81 0.9125053866419961 1107 82-83 0.9591928282171267 1107 84-85 1.0669854385327504 1107 86-87 1.1794508950150941 1107 88-89 1.3620831187615892 1107 90-91 1.5467297642524187 1107 92-93 1.603956022453648 1107 94-95 1.796158658107725 1107 96-97 1.7841646061858007 1107 98-99 1.7827604847388159 1107 100-101 1.8115157709010958 1108 1 0.1235625017320956 1108 2 0.17530599930272928 1108 3 0.1700891405616929 1108 4 0.07276861482520047 1108 5 -0.02517839228045915 1108 6 0.08728971438344502 1108 7 0.09377415415674051 1108 8 0.09939175568398184 1108 9 0.19040584950391093 1108 10-11 0.16956220391922727 1108 12-13 0.22203058798152142 1108 14-15 0.2781910841974238 1108 16-17 0.21547944098530536 1108 18-19 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66-67 -1.1090879187231586 2316 68-69 -1.0666581012509049 2316 70-71 -1.223848379261085 2316 72-73 -1.2712231444986521 2316 74-75 -1.1819654951197904 2316 76-77 -1.3764333650748313 2316 78-79 -1.4544941648494003 2316 80-81 -1.3434540278623288 2316 82-83 -1.4303108311308428 2316 84-85 -1.5101578304847685 2316 86-87 -1.4968476190678537 2316 88-89 -1.541882727324019 2316 90-91 -1.5328912160092827 2316 92-93 -1.9665520724676178 2316 94-95 -1.695009228341597 2316 96-97 -1.8474563740759002 2316 98-99 -1.9720259424068907 2316 100-101 -2.290558303172979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 17.0 4 58.0 5 101.0 6 203.0 7 533.0 8 1296.0 9 2444.0 10 4041.0 11 6170.0 12 8749.0 13 12062.0 14 15880.0 15 20764.0 16 25197.0 17 31562.0 18 30367.0 19 27566.0 20 27821.0 21 29587.0 22 32344.0 23 36561.0 24 42080.0 25 49006.0 26 58432.0 27 69802.0 28 83030.0 29 98569.0 30 117208.0 31 139772.0 32 167426.0 33 205977.0 34 260038.0 35 336677.0 36 440791.0 37 491321.0 38 237291.0 39 9170.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.81859966293856 19.442124217845024 15.08465319147436 23.654622927742054 2 15.823050500236835 20.47727183078311 39.4373975177529 24.26228015122716 3 19.19042051132146 23.56736527521895 30.337771914372052 26.90444229908754 4 13.210387715566702 17.0090797316338 36.80044590944115 32.98008664335835 5 13.64642827480961 37.49385517747594 34.6259506637691 14.233765883945345 6 32.7260413421387 36.6039342084851 16.9313853321692 13.738639117207002 7 28.8203913182278 32.563056184846 20.33586801695943 18.28068447996677 8 26.464911234789657 35.56316900839638 19.437702809947446 18.53421694686652 9 26.006789926395452 16.679199055359184 19.855566624229624 37.458444394015736 10-11 24.874788295582317 26.55734962095133 27.663356962174625 20.904505121291727 12-13 25.79479703940747 23.648345019545395 27.79988307377506 22.756974867272067 14-15 22.2522657661296 25.413456644345555 25.882139134515157 26.45213845500969 16-17 22.116781349241453 28.563701715046175 26.923256908198812 22.396260027513563 18-19 22.150612388282248 27.032731009424616 28.97911354023634 21.8375430620568 20-21 22.476326285707692 26.36337003944978 29.265707794697036 21.89459588014549 22-23 22.205613869091348 26.29803174188023 29.17341364318783 22.322940745840594 24-25 22.182882903385973 26.534984035441607 29.196678679485327 22.085454381687093 26-27 21.840865834278354 26.900551243037345 28.868669663811623 22.389913258872678 28-29 21.962006632877262 27.2405833111372 28.755833896650323 22.041576159335214 30-31 22.152801483397518 26.785197436427538 28.651560243792602 22.410440836382346 32-33 21.7277038011426 26.778836784721584 28.728175610531608 22.765283803604213 34-35 22.500361032471126 26.963523779497876 28.510810378052685 22.025304809978312 36-37 22.693701117969926 26.466743428281887 28.54666474094718 22.292890712801004 38-39 22.096242231181947 26.775298832748057 28.560882900258306 22.56757603581169 40-41 22.69793303013801 26.629717607819014 28.555175662148653 22.117173699894323 42-43 22.353244356378138 26.679944991324163 28.398124730457646 22.56868592184005 44-45 22.278115101078388 26.91339884051393 28.63576712575545 22.172718932652234 46-47 22.68093115327464 27.1155216999432 27.878186463994542 22.32536068278761 48-49 22.38911239917998 27.248265658434395 27.776581164364682 22.586040778020948 50-51 22.622039167922047 27.305218115965257 27.866502131478573 22.206240584634124 52-53 22.9485345118266 26.93380206390172 27.86241680865767 22.25524661561401 54-55 22.24593760922936 27.263729224484944 28.045460890418937 22.444872275866757 56-57 22.39545872388872 27.339758506318763 27.77292721983541 22.491855549957116 58-59 22.313829143482856 26.972626979904526 27.83222666052617 22.881317216086448 60-61 22.031394434978377 27.21940270186761 28.128209221017492 22.62099364213652 62-63 22.191803611852475 27.18914623521213 28.16723073145494 22.45181942148045 64-65 21.87294132732795 27.250118210888225 28.030630645070808 22.846309816713013 66-67 21.780989038745837 27.31920149141781 27.952352073073317 22.94745739676304 68-69 22.415694443509434 26.879281476516137 28.045388259333453 22.65963582064098 70-71 22.18297077787573 26.96190134758544 27.684421378990155 23.170706495548675 72-73 22.099336039603642 27.093638769004926 27.73070740709282 23.07631778429861 74-75 22.188091248587174 27.367266570669628 27.504257110051043 22.94038507069215 76-77 22.137367248527816 27.054875899801402 27.800596281275187 23.007160570395598 78-79 22.24908506297271 27.24643912392405 27.63546670886062 22.869009104242625 80-81 22.449526261412647 27.234971293549602 27.4258774042604 22.889625040777346 82-83 22.340617063638707 27.34125113086009 27.497675754856626 22.820456050644577 84-85 22.2967785326553 27.456188127460994 27.42283253569497 22.82420080418873 86-87 22.429264391341594 27.416029638153915 27.145099855671873 23.00960611483262 88-89 22.151910713294683 27.499399308169824 26.995010648846623 23.35367932968887 90-91 22.12066209154486 27.453375804775426 27.186120552445075 23.239841551234637 92-93 22.22530889924787 27.143074693665564 27.390755144046114 23.24086126304045 94-95 21.911858248873695 27.006384007407398 27.50520672359864 23.576551020120267 96-97 22.186936711977943 26.59855358978852 27.532328113133524 23.68218158510001 98-99 21.876957766678387 26.911048863451047 27.16331063266039 24.048682737210168 100-101 21.981564781641975 26.590738024224674 27.609743212295445 23.817953981837906 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 286.0 1 203.5 2 169.0 3 227.5 4 216.5 5 219.5 6 363.0 7 825.0 8 1366.0 9 1584.0 10 1435.0 11 1284.0 12 1365.0 13 1525.0 14 1783.0 15 1984.0 16 2200.5 17 2529.5 18 2833.0 19 3324.5 20 4001.5 21 4911.0 22 5908.0 23 7377.5 24 9742.5 25 12848.5 26 17003.0 27 21747.5 28 26022.5 29 30993.5 30 36761.5 31 44189.0 32 55933.0 33 61391.0 34 61850.5 35 66474.0 36 72915.5 37 79542.5 38 84743.5 39 89245.0 40 94098.5 41 100173.0 42 105160.0 43 109438.5 44 113030.0 45 117421.5 46 119405.5 47 120372.5 48 121200.5 49 118387.0 50 115036.5 51 111084.5 52 107200.0 53 106564.5 54 107190.0 55 99245.0 56 88489.5 57 80327.5 58 73793.5 59 64768.5 60 51496.0 61 42279.5 62 36367.0 63 27580.0 64 18426.0 65 13242.5 66 9805.5 67 7430.0 68 5579.5 69 4117.5 70 3189.5 71 2293.0 72 1646.5 73 1353.5 74 1074.0 75 808.0 76 615.0 77 391.0 78 248.5 79 166.5 80 107.5 81 69.5 82 35.0 83 14.0 84 6.0 85 2.5 86 0.5 87 1.0 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.2052144993647267E-4 2 0.054264281474244815 3 0.0 4 0.0 5 0.04605893235587112 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006186063983773922 26-27 0.0018590244096315413 28-29 9.615643498094179E-5 30-31 0.0016346593946760103 32-33 3.8462573992376715E-4 34-35 0.012516362620019258 36-37 0.023574352642827562 38-39 0.004887952111531208 40-41 0.0011218250747776542 42-43 0.0014263204522173031 44-45 0.008269453408360994 46-47 0.0 48-49 0.0 50-51 5.128343198983563E-4 52-53 0.0 54-55 0.001522476887198245 56-57 0.0 58-59 0.023446144062852975 60-61 0.0 62-63 0.009551539208106885 64-65 0.015737603191880806 66-67 0.0064104289987294534 68-69 0.01663506325170293 70-71 8.013036248411817E-5 72-73 3.205214499364727E-5 74-75 0.023157674757910147 76-77 0.0509308583949055 78-79 0.031587388891239375 80-81 0.027035984302141467 82-83 0.037228566410121296 84-85 0.015994020351829986 86-87 0.009247043830667236 88-89 0.08580359214799373 90-91 0.025657742067414637 92-93 0.02487246451507028 94-95 0.027949470434460416 96-97 0.039408112269689315 98-99 0.055338028331532 100-101 0.014487569537128563 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3119916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.79114302870808 #Duplication Level Percentage of deduplicated Percentage of total 1 71.61435812246818 36.37375106307403 2 15.228555446716378 15.469514756295657 3 5.580892526361849 8.503797316028779 4 2.621330780691737 5.325615464306761 5 1.4026311204975022 3.5620618928852856 6 0.8568988817807136 2.6113724199398543 7 0.5713366159561778 2.0313187844997795 8 0.38509220830234187 1.5647418744900232 9 0.2694120749484016 1.231537250912876 >10 1.3466825950013062 12.085299002404284 >50 0.07773129411499526 2.7162635238420187 >100 0.0387062385138371 3.682758622884083 >500 0.003028322208949001 1.0331356177297883 >1k 0.0032175923456436396 3.3856572932109703 >5k 1.261800919860251E-4 0.4231751174958741 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7230 0.2317370083040697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5976 0.19154361848203605 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4487 0.14381797458649528 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4480 0.14359360957153974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4172 0.13372154891349639 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3980 0.1275675370747161 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3942 0.1263495555649575 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3845 0.12324049750057373 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3795 0.12163789025089136 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.205214499364727E-5 1.2820857997458907E-4 14-15 0.0 0.0 0.0 6.410428998729453E-5 1.6026072496823634E-4 16-17 0.0 0.0 0.0 6.410428998729453E-5 1.6026072496823634E-4 18-19 0.0 0.0 0.0 1.2820857997458907E-4 1.6026072496823634E-4 20-21 0.0 0.0 0.0 1.923128699618836E-4 1.6026072496823634E-4 22-23 0.0 0.0 0.0 4.647561024078853E-4 1.6026072496823634E-4 24-25 0.0 0.0 0.0 7.051471898602399E-4 1.6026072496823634E-4 26-27 0.0 0.0 0.0 0.0013782422347268324 1.6026072496823634E-4 28-29 1.6026072496823634E-5 0.0 0.0 0.004439222081620146 1.6026072496823634E-4 30-31 3.205214499364727E-5 0.0 0.0 0.0134939530423255 1.6026072496823634E-4 32-33 3.205214499364727E-5 0.0 0.0 0.031074554571341024 1.6026072496823634E-4 34-35 3.205214499364727E-5 0.0 0.0 0.05846311246841261 1.9231286996188358E-4 36-37 3.205214499364727E-5 0.0 0.0 0.10513103557916303 1.9231286996188358E-4 38-39 3.205214499364727E-5 0.0 0.0 0.17817787401968516 1.9231286996188358E-4 40-41 3.205214499364727E-5 0.0 0.0 0.25441390088707516 1.9231286996188358E-4 42-43 3.205214499364727E-5 0.0 0.0 0.3380860253929914 1.9231286996188358E-4 44-45 3.205214499364727E-5 0.0 0.0 0.4410054629675927 2.083389424587072E-4 46-47 3.205214499364727E-5 0.0 0.0 0.560111233763986 2.2436501495553084E-4 48-49 3.205214499364727E-5 0.0 0.0 0.6861082157340134 2.2436501495553084E-4 50-51 3.205214499364727E-5 0.0 0.0 0.8260478807762773 2.2436501495553084E-4 52-53 3.205214499364727E-5 0.0 0.0 0.9620130798393289 2.2436501495553084E-4 54-55 3.205214499364727E-5 0.0 0.0 1.1119850662646045 2.2436501495553084E-4 56-57 3.205214499364727E-5 0.0 0.0 1.2823422169058398 2.2436501495553084E-4 58-59 3.205214499364727E-5 0.0 0.0 1.4685331271739366 2.2436501495553084E-4 60-61 3.205214499364727E-5 0.0 0.0 1.6690513462541938 2.2436501495553084E-4 62-63 3.205214499364727E-5 0.0 0.0 1.86942533068198 2.2436501495553084E-4 64-65 3.205214499364727E-5 0.0 0.0 2.080488705465147 2.2436501495553084E-4 66-67 3.205214499364727E-5 0.0 0.0 2.3268094397413264 2.403910874523545E-4 68-69 3.205214499364727E-5 0.0 0.0 2.5954064147880906 2.5641715994917814E-4 70-71 3.205214499364727E-5 0.0 0.0 2.8786191679519577 2.5641715994917814E-4 72-73 3.205214499364727E-5 0.0 0.0 3.1775534982352087 2.884693049428254E-4 74-75 4.80782174904709E-5 0.0 0.0 3.4776417057382316 3.365475224332963E-4 76-77 6.410428998729453E-5 0.0 0.0 3.8066409480255237 3.5257359493011994E-4 78-79 6.410428998729453E-5 0.0 0.0 4.177836839196953 3.685996674269435E-4 80-81 6.410428998729453E-5 0.0 0.0 4.57023522428168 3.8462573992376715E-4 82-83 6.410428998729453E-5 0.0 0.0 4.972649904676921 3.8462573992376715E-4 84-85 6.410428998729453E-5 0.0 0.0 5.387645051982169 3.8462573992376715E-4 86-87 6.410428998729453E-5 0.0 0.0 5.811262226290708 4.006518124205908E-4 88-89 6.410428998729453E-5 0.0 0.0 6.258213362154622 4.166778849174144E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4285 0.0 57.236633 1 GTATCAA 7690 0.0 40.731865 1 ATCAACG 9780 0.0 31.670439 3 TCAACGC 9990 0.0 30.957142 4 TATCAAC 10175 0.0 30.503801 2 CAACGCA 10275 0.0 30.190945 5 AACGCAG 10820 0.0 28.657831 6 ACGCAGA 12480 0.0 24.731825 7 CGCAGAG 12475 0.0 24.627544 8 TAAGGTG 2290 0.0 23.856556 5 GTACATG 9275 0.0 23.778217 1 TACATGG 9100 0.0 23.504246 2 GCGTATC 105 0.0021887962 22.633718 1 GGTAAGG 2235 0.0 22.105543 3 GTAAGGT 2235 0.0 22.105543 4 ACATGGG 9055 0.0 22.087116 3 GAGTACT 9200 0.0 21.161839 12-13 AGGTAAG 2125 0.0 21.025394 2 GCAGAGT 14535 0.0 20.97381 9 GTACTTT 9630 0.0 20.068989 14-15 >>END_MODULE