FastQCFastQC Report
Thu 26 May 2016
SRR938418_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938418_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091122
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71950.23276337847551795No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56260.18200511011859125No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46160.14933089020750395No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA43140.139560974946961No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41580.13451426375277326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39290.1271059505254079No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39170.12671774197200888No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA33250.10756612000432206No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG33180.10733966501483927No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33160.10727496358927276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43400.049.5731771
GTATCAA79900.043.1906051
ATCAACG100000.033.97933
TATCAAC102900.033.2064062
TCAACGC103500.032.692494
CAACGCA105700.032.009455
AACGCAG111200.030.3781056
ACGCAGA125150.026.837087
CGCAGAG126200.026.2754258
TGGTATC21550.023.8168812
GTACATG95850.023.091861
TACATGG94100.022.9284462
GCAGAGT142200.022.7172629
ACATGGG91750.022.3243893
TAAGGTG19900.021.730095
GTGGTAT26950.021.5476111
AGAGTAC126600.020.67523810-11
GAGTACT86700.020.18953712-13
CAGAGTA132300.019.99990710-11
GTGTAAG15250.019.6638581
Produced by FastQC (version 0.11.5)