##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938418_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3091122 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.070523583346112 31.0 31.0 34.0 28.0 34.0 2 31.16962449233644 31.0 31.0 34.0 27.0 34.0 3 31.239040710783982 31.0 31.0 34.0 27.0 34.0 4 34.86531557149799 37.0 35.0 37.0 32.0 37.0 5 34.89194344318988 37.0 35.0 37.0 32.0 37.0 6 34.921528493537295 37.0 35.0 37.0 32.0 37.0 7 34.84223010285586 37.0 35.0 37.0 32.0 37.0 8 34.80154552295251 37.0 35.0 37.0 32.0 37.0 9 36.31451621773583 39.0 35.0 39.0 32.0 39.0 10-11 36.17150633329904 39.0 35.0 39.0 31.5 39.0 12-13 36.02794939830909 39.0 35.0 39.0 30.0 39.0 14-15 37.03111119522296 39.0 36.0 41.0 31.0 41.0 16-17 36.879062198127414 39.0 36.0 41.0 31.0 41.0 18-19 36.87486210508676 39.0 36.0 41.0 30.5 41.0 20-21 36.95792967731458 39.0 36.0 41.0 30.5 41.0 22-23 36.88245934647678 39.0 36.0 41.0 30.0 41.0 24-25 36.77384603389966 39.0 36.0 41.0 30.0 41.0 26-27 36.59139545446605 39.0 36.0 41.0 30.0 41.0 28-29 36.410935576143544 39.0 36.0 41.0 29.5 41.0 30-31 36.19302715971741 39.0 35.0 40.0 29.0 41.0 32-33 35.91581050505286 38.5 35.0 40.0 27.5 41.0 34-35 35.66819620836706 38.0 35.0 40.0 27.0 41.0 36-37 35.38455890773642 38.0 34.0 40.0 26.5 41.0 38-39 35.12890012105637 38.0 34.0 40.0 25.5 41.0 40-41 35.05114955022804 38.0 34.0 40.0 25.0 41.0 42-43 34.871547127547856 38.0 34.0 40.0 24.5 41.0 44-45 34.60050865025709 38.0 33.0 40.0 24.0 41.0 46-47 34.2622736986764 38.0 33.0 40.0 23.5 41.0 48-49 33.91606769321949 38.0 33.0 40.0 22.5 41.0 50-51 33.18008461005421 37.5 32.0 39.5 20.0 40.5 52-53 33.23659580566539 37.0 32.5 39.5 20.0 40.5 54-55 33.36636227881009 37.5 32.5 40.0 19.5 41.0 56-57 33.074535880499056 37.0 32.0 40.0 17.5 41.0 58-59 32.74741954539485 37.0 31.5 40.0 15.0 41.0 60-61 32.306104870658615 36.0 31.0 39.0 12.0 41.0 62-63 31.794799914076506 36.0 30.5 39.0 8.0 40.5 64-65 31.309210862592934 35.0 30.0 39.0 7.0 40.0 66-67 30.78264494251602 35.0 29.0 38.0 4.0 40.0 68-69 30.22822748503618 34.5 28.5 38.0 2.0 40.0 70-71 29.60309007538363 34.0 28.0 37.0 2.0 39.5 72-73 29.04796413729384 34.0 27.5 36.5 2.0 39.0 74-75 28.437992094779823 34.0 26.0 36.0 2.0 39.0 76-77 27.7065112603126 33.0 25.5 35.5 2.0 38.0 78-79 26.922955968738858 32.5 24.5 35.0 2.0 37.0 80-81 26.274156277235257 32.0 22.5 35.0 2.0 36.5 82-83 25.604212806870777 31.5 20.5 35.0 2.0 36.0 84-85 24.940298862354833 31.0 18.5 34.0 2.0 36.0 86-87 24.262789207284605 31.0 13.5 34.0 2.0 35.0 88-89 23.4576951346469 30.5 4.5 34.0 2.0 35.0 90-91 22.625064620548784 29.5 2.0 34.0 2.0 35.0 92-93 21.78143567287218 29.0 2.0 34.0 2.0 35.0 94-95 20.811664502403982 29.0 2.0 34.0 2.0 35.0 96-97 19.581704960205386 26.5 2.0 33.5 2.0 35.0 98-99 18.096507514100058 24.5 2.0 33.0 2.0 34.5 100-101 14.62248093087235 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5167134856547868 1101 2 0.5874296806400103 1101 3 0.604212574985926 1101 4 0.5165221040961328 1101 5 0.45009026597442414 1101 6 0.4653975506378032 1101 7 0.3628257879568082 1101 8 0.5115829425719696 1101 9 0.4028147795389003 1101 10-11 0.5652953293357257 1101 12-13 0.6591048228209431 1101 14-15 0.7078074226265656 1101 16-17 0.640749903188798 1101 18-19 0.6391450784587249 1101 20-21 0.7296646042360777 1101 22-23 0.7101948771957467 1101 24-25 0.8550864505730971 1101 26-27 0.8276428651260019 1101 28-29 0.8066515956600284 1101 30-31 0.7324978540436078 1101 32-33 0.8069197904025103 1101 34-35 0.7717120272358002 1101 36-37 0.8370678921251198 1101 38-39 0.715017682188666 1101 40-41 0.7698277207800501 1101 42-43 0.9113232660685284 1101 44-45 0.7614748559424029 1101 46-47 0.7641932435946117 1101 48-49 0.697818927272472 1101 50-51 0.7224030772667973 1101 52-53 0.8685809043531165 1101 54-55 0.9781819588048606 1101 56-57 0.9601258129346064 1101 58-59 0.9067749159822966 1101 60-61 0.9257408439631192 1101 62-63 0.7990031376442879 1101 64-65 0.7920328109854609 1101 66-67 0.765273160871633 1101 68-69 0.9239473454216913 1101 70-71 0.9857196033839521 1101 72-73 0.99900229128232 1101 74-75 0.8935616718257506 1101 76-77 0.9121478247483346 1101 78-79 0.7851395687817444 1101 80-81 0.9889485426355442 1101 82-83 0.9974409223305578 1101 84-85 1.1118124685683455 1101 86-87 1.2576760883036826 1101 88-89 1.3974513305965388 1101 90-91 1.0553033927795532 1101 92-93 1.0886968836054436 1101 94-95 1.3178594050194867 1101 96-97 1.3498361626132755 1101 98-99 1.3552742807694038 1101 100-101 1.102390897541257 1102 1 0.4451766664437713 1102 2 0.5467935336099146 1102 3 0.4355015804784941 1102 4 0.5155288393641584 1102 5 0.45486681954319863 1102 6 0.4409854005067828 1102 7 0.43607771605233836 1102 8 0.43375544672515787 1102 9 0.6352651959339539 1102 10-11 0.5741695485529306 1102 12-13 0.6106042084234602 1102 14-15 0.6769532957102982 1102 16-17 0.7194655724110035 1102 18-19 0.6983637774972635 1102 20-21 0.5402749506091098 1102 22-23 0.7052835105953719 1102 24-25 0.6182411517794151 1102 26-27 0.6362086967294971 1102 28-29 0.4807792252431611 1102 30-31 0.6049708948020083 1102 32-33 0.5444596791335314 1102 34-35 0.6546235445755357 1102 36-37 0.7448404129673065 1102 38-39 0.779004747315696 1102 40-41 0.7591623500531881 1102 42-43 0.7910046541986091 1102 44-45 0.9078224770643928 1102 46-47 0.763541161260413 1102 48-49 0.7834590738306133 1102 50-51 0.8619748294279574 1102 52-53 0.9763864667253443 1102 54-55 0.9133862398027688 1102 56-57 0.8481152504120928 1102 58-59 0.8457790850921256 1102 60-61 0.8609565650019775 1102 62-63 0.8498656718690789 1102 64-65 0.8008806732455724 1102 66-67 0.7925834547102042 1102 68-69 0.8293752186552688 1102 70-71 0.8144557973855093 1102 72-73 0.9205210307423428 1102 74-75 0.6625035757087687 1102 76-77 0.611014542887709 1102 78-79 0.5840901581233062 1102 80-81 0.7065693012403607 1102 82-83 0.7280050778558937 1102 84-85 0.6164853888249766 1102 86-87 0.6215982592376363 1102 88-89 0.8207472019081905 1102 90-91 0.6193433961352959 1102 92-93 0.675072781816894 1102 94-95 0.5675400932882368 1102 96-97 0.5132764386457609 1102 98-99 0.5560810952653235 1102 100-101 0.7189289123487068 1103 1 0.5814886346731676 1103 2 0.6158386486305858 1103 3 0.40062107851508344 1103 4 0.5277576995007891 1103 5 0.4976728370156067 1103 6 0.55924326026377 1103 7 0.5203865973029025 1103 8 0.4137864411320109 1103 9 0.41363293362265097 1103 10-11 0.5348876761418282 1103 12-13 0.5124273996634869 1103 14-15 0.7064044356804899 1103 16-17 0.6504784272566866 1103 18-19 0.6981734004505711 1103 20-21 0.5355033963891529 1103 22-23 0.6630758845648757 1103 24-25 0.787210182741326 1103 26-27 0.680000646996028 1103 28-29 0.5643102421258561 1103 30-31 0.6339754108882616 1103 32-33 0.8823851679385584 1103 34-35 0.9294622414752425 1103 36-37 0.8302130023221252 1103 38-39 0.7276591343105068 1103 40-41 0.9138445349897495 1103 42-43 0.7964415823164259 1103 44-45 0.7690070117277088 1103 46-47 0.9623862591015282 1103 48-49 0.9148295838363651 1103 50-51 1.0519575475092537 1103 52-53 0.9698007116016285 1103 54-55 0.9704726498413265 1103 56-57 1.1132096689709954 1103 58-59 0.9540620109748161 1103 60-61 1.0431030033378406 1103 62-63 1.0904441777552982 1103 64-65 0.9965948419710386 1103 66-67 1.1303556182257317 1103 68-69 1.198188743503536 1103 70-71 1.2393061550184186 1103 72-73 1.3525440744874473 1103 74-75 1.2220417439143958 1103 76-77 1.1037660842630608 1103 78-79 1.1041074533939899 1103 80-81 1.0588677563712388 1103 82-83 1.1358599168598893 1103 84-85 1.1136664510821674 1103 86-87 1.2565825951344003 1103 88-89 1.277536730569782 1103 90-91 1.4446300076107939 1103 92-93 1.4042554801372589 1103 94-95 1.230152466928395 1103 96-97 1.0725127407335222 1103 98-99 1.184791333611603 1103 100-101 0.880490151003416 1104 1 0.5972755699996739 1104 2 0.5635398279547168 1104 3 0.5869919020974734 1104 4 0.6534572515827719 1104 5 0.5076802056592982 1104 6 0.6311485151983689 1104 7 0.5888482479473183 1104 8 0.6007239850539037 1104 9 0.5850178633817364 1104 10-11 0.5997596715432465 1104 12-13 0.6840662661886867 1104 14-15 0.778248498464059 1104 16-17 0.8620283284963932 1104 18-19 0.8188414439051002 1104 20-21 0.8681948873113967 1104 22-23 0.7797594021711589 1104 24-25 0.886565447739585 1104 26-27 0.8637451119847341 1104 28-29 0.9473881390944783 1104 30-31 0.9101061650825883 1104 32-33 0.7745284582085432 1104 34-35 0.8550244416976724 1104 36-37 0.9986223304792148 1104 38-39 1.0062072982901213 1104 40-41 0.860560030292234 1104 42-43 0.9327569065367456 1104 44-45 1.062025093901724 1104 46-47 1.0527935045933603 1104 48-49 1.0027810411903388 1104 50-51 0.7955861013447034 1104 52-53 1.0426968742780858 1104 54-55 1.2513303377929148 1104 56-57 1.1052967989183884 1104 58-59 1.0157011632055344 1104 60-61 1.1061378483234066 1104 62-63 1.2003252389764434 1104 64-65 1.0661249033081717 1104 66-67 1.1511005036706088 1104 68-69 1.172649229151741 1104 70-71 1.2432755427387114 1104 72-73 1.1399997474902364 1104 74-75 1.0623449414281652 1104 76-77 1.1561134109123046 1104 78-79 1.1640944175469023 1104 80-81 1.254766325796826 1104 82-83 1.150818732623332 1104 84-85 1.1171669832619884 1104 86-87 1.2505712658447266 1104 88-89 1.264323596815256 1104 90-91 1.1905559322732344 1104 92-93 1.2495446652880773 1104 94-95 1.2379042545067698 1104 96-97 1.3179745005430306 1104 98-99 0.8820254697063561 1104 100-101 0.6565560884245247 1105 1 0.5086764013155118 1105 2 0.5158623215815616 1105 3 0.4841113566516704 1105 4 0.4717970048334834 1105 5 0.3411661205877081 1105 6 0.438344367437395 1105 7 0.37904250890192515 1105 8 0.3855538739445734 1105 9 0.48322255787115864 1105 10-11 0.5025355008549468 1105 12-13 0.5480532352929188 1105 14-15 0.6939979052396481 1105 16-17 0.6836959003647323 1105 18-19 0.5800242752690394 1105 20-21 0.5904749734090942 1105 22-23 0.6630278181818028 1105 24-25 0.5862749982518451 1105 26-27 0.7809875002741649 1105 28-29 0.7102524410136368 1105 30-31 0.7862957203246026 1105 32-33 0.8421547889552059 1105 34-35 0.7205649172391873 1105 36-37 0.9477501302579583 1105 38-39 0.8162545357883175 1105 40-41 0.804398512920379 1105 42-43 0.7546109633099221 1105 44-45 0.818860702598073 1105 46-47 0.7629209075530596 1105 48-49 0.5742244002835122 1105 50-51 0.5389887844827044 1105 52-53 0.5492550603741861 1105 54-55 0.6109895744573848 1105 56-57 0.6994844522348274 1105 58-59 0.8893455151479444 1105 60-61 0.6354745492741714 1105 62-63 0.6728880085328122 1105 64-65 0.8923692716845011 1105 66-67 0.7926329766949323 1105 68-69 0.7846508003947825 1105 70-71 0.736250543139537 1105 72-73 0.7019832314010301 1105 74-75 0.6852178511137552 1105 76-77 0.7204004493596621 1105 78-79 0.8119195786794791 1105 80-81 0.7607694662787843 1105 82-83 0.7501959471942037 1105 84-85 0.6717955365960684 1105 86-87 0.7921164150326483 1105 88-89 0.9404350408357125 1105 90-91 0.870923811576116 1105 92-93 0.9285701739379313 1105 94-95 0.7805028780657963 1105 96-97 0.830208251003981 1105 98-99 0.7626578449288139 1105 100-101 0.6104748768037407 1106 1 0.28586433288627333 1106 2 0.384218900119663 1106 3 0.1942366443655139 1106 4 0.3760841587992658 1106 5 0.4326756695768239 1106 6 0.09204315437362709 1106 7 0.3360510314524703 1106 8 0.3868134695899812 1106 9 0.4880673479178128 1106 10-11 0.27451833133706316 1106 12-13 0.1921659569103369 1106 14-15 0.5686479830001474 1106 16-17 0.22149322540361283 1106 18-19 0.08402128926592667 1106 20-21 0.3448125161773774 1106 22-23 0.43014172175878684 1106 24-25 0.4120397260901001 1106 26-27 0.2460935655929788 1106 28-29 0.2383828298161248 1106 30-31 0.20092688673206993 1106 32-33 0.35562191181679026 1106 34-35 0.25654650355333786 1106 36-37 0.12743635109777074 1106 38-39 0.16400719519733542 1106 40-41 0.47649157668398345 1106 42-43 -0.02637541305087865 1106 44-45 0.2695588052419282 1106 46-47 0.2351971128407797 1106 48-49 0.5058974329429233 1106 50-51 0.1392901219632563 1106 52-53 0.36543496706159573 1106 54-55 0.07581147181353032 1106 56-57 -0.16568531448367452 1106 58-59 -0.49387837287694225 1106 60-61 0.190952745230625 1106 62-63 0.22561410495582734 1106 64-65 0.35275521258652276 1106 66-67 -0.005754083958255052 1106 68-69 -0.2127899461059961 1106 70-71 -0.3249804366427327 1106 72-73 0.2840251598613719 1106 74-75 0.2678174156549673 1106 76-77 0.2897936686442648 1106 78-79 0.4388257062066927 1106 80-81 0.35140287218857935 1106 82-83 0.0911126501491033 1106 84-85 0.03179976676714347 1106 86-87 0.14991374007152913 1106 88-89 0.11569127722762573 1106 90-91 0.2941596436134404 1106 92-93 0.3816568302365333 1106 94-95 -0.0097525496169375 1106 96-97 0.23700862425437208 1106 98-99 0.2217498511496565 1106 100-101 -0.22567143734866946 1107 1 0.2873936860596693 1107 2 0.3323217607330875 1107 3 0.23890759486657487 1107 4 0.3357778605909445 1107 5 0.3587270998835308 1107 6 0.32919337605598287 1107 7 0.2727469075532909 1107 8 0.26904064238075165 1107 9 0.24407244964876185 1107 10-11 0.33938533407012983 1107 12-13 0.23957911208798777 1107 14-15 0.33077977771620937 1107 16-17 0.4590372035390473 1107 18-19 0.40028181765949 1107 20-21 0.4736825313610922 1107 22-23 0.5135895687068555 1107 24-25 0.39824600607795446 1107 26-27 0.4513704559665044 1107 28-29 0.4883008862995766 1107 30-31 0.5521750373546013 1107 32-33 0.4537733360500127 1107 34-35 0.5593390453085831 1107 36-37 0.633421701841776 1107 38-39 0.6305990688687615 1107 40-41 0.5371694266688039 1107 42-43 0.5944342559439519 1107 44-45 0.6772348210330037 1107 46-47 0.5496357402322118 1107 48-49 0.5638647089830044 1107 50-51 0.4652416069313716 1107 52-53 0.32329476663644385 1107 54-55 0.3845012744251335 1107 56-57 0.4047587061661915 1107 58-59 0.39030655272281223 1107 60-61 0.4586792924520111 1107 62-63 0.37425459083496193 1107 64-65 0.3116388566800552 1107 66-67 0.40723919876266734 1107 68-69 0.3653377186374023 1107 70-71 0.37622439785468487 1107 72-73 0.35157421543379996 1107 74-75 0.5018874065447321 1107 76-77 0.39700537833148175 1107 78-79 0.5402916521526393 1107 80-81 0.4597170902268424 1107 82-83 0.5213824831281499 1107 84-85 0.4533488952214384 1107 86-87 0.40651025388871886 1107 88-89 0.6424638979382244 1107 90-91 0.8519146876462393 1107 92-93 0.7895248168748559 1107 94-95 0.7400762508689418 1107 96-97 0.5889537684997386 1107 98-99 0.5252833585896788 1107 100-101 0.0877285262103964 1108 1 0.3834137807390334 1108 2 0.39076810257365224 1108 3 0.4903779940587647 1108 4 0.43316244100785184 1108 5 0.3158643812332613 1108 6 0.3231896574411124 1108 7 0.3698934854402012 1108 8 0.3280483162157495 1108 9 0.2500722558932793 1108 10-11 0.4004731779628301 1108 12-13 0.46632742930297866 1108 14-15 0.5583719093805186 1108 16-17 0.5175737162011558 1108 18-19 0.5596269334377055 1108 20-21 0.49257546096264804 1108 22-23 0.5788666297342431 1108 24-25 0.5532205849858087 1108 26-27 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2316 68-69 -0.7628446205891812 2316 70-71 -1.1048032567599826 2316 72-73 -0.9942418935172128 2316 74-75 -0.7458432569769364 2316 76-77 -0.7012739277765938 2316 78-79 -0.6643707393348066 2316 80-81 -0.7413251006593065 2316 82-83 -0.6157387167129791 2316 84-85 -0.6429576044145904 2316 86-87 -0.7809372812181969 2316 88-89 -1.0682782136702436 2316 90-91 -1.0840383446617814 2316 92-93 -0.9517009729106469 2316 94-95 -1.159446199724929 2316 96-97 -1.2492374973130467 2316 98-99 -1.276905477452626 2316 100-101 -1.1367640137139894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10377.0 3 3665.0 4 3629.0 5 4230.0 6 4718.0 7 6020.0 8 7941.0 9 9975.0 10 12584.0 11 15526.0 12 18985.0 13 22489.0 14 25571.0 15 29811.0 16 33377.0 17 37567.0 18 35921.0 19 35082.0 20 36758.0 21 39948.0 22 43987.0 23 48956.0 24 54869.0 25 61383.0 26 69586.0 27 79033.0 28 91147.0 29 103800.0 30 120818.0 31 141879.0 32 167365.0 33 201149.0 34 247793.0 35 307132.0 36 371233.0 37 381514.0 38 193705.0 39 11599.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.173725591625356 18.60964556605908 14.513498464013711 23.70313037830185 2 15.373174763418785 19.661301343148846 40.69653290418513 24.268990989247236 3 19.238406512685618 23.401274145108058 29.81074255219363 27.549576790012694 4 12.759659284938587 16.30175585108363 37.19403700089176 33.74454786308602 5 13.702944310717427 37.31375509353194 34.63064367223745 14.35265692351318 6 32.767807774832605 36.09268757583252 17.276315908740695 13.863188740594175 7 28.906050005647376 32.471631243545566 20.423659945804616 18.19865880500244 8 26.360427756260226 35.25898515979371 19.69091102089849 18.689676063047575 9 26.171121385075708 16.776517535953033 20.02414399660819 37.02821708236307 10-11 24.882494101038 26.381067523999196 27.768676863754006 20.967761511208796 12-13 25.921495059722027 23.537581761483096 27.811000284317412 22.72992289447746 14-15 22.41806522225622 25.32854736248681 25.90463085235988 26.348756562897098 16-17 22.244460118304744 28.44596911300244 26.910599612533115 22.398971156159707 18-19 22.32388971364268 26.955499867143978 28.917626353197655 21.80298406601569 20-21 22.589806271084537 26.278075965428034 29.259531687596446 21.872586075890982 22-23 22.274582699011663 26.22951323745103 29.077904114353164 22.41799994918414 24-25 22.162003443272443 26.41685351718172 29.232237006955824 22.188906032590012 26-27 21.7818942715521 26.80333118136139 28.878627325663164 22.53614722142335 28-29 21.935936156567493 27.2676635001611 28.710804844573882 22.085595498697522 30-31 22.099808667792455 26.748880280935627 28.635368588825433 22.515942462446482 32-33 21.715374006273493 26.724263774123187 28.718141761776234 22.84222045782709 34-35 22.511030796897394 26.967401535248705 28.43511573921228 22.08645192864162 36-37 22.700833665360246 26.43716405538694 28.45360043940835 22.408401839844462 38-39 22.15572485785602 26.74369782910168 28.45360000737801 22.64697730566429 40-41 22.725734196761753 26.59632542432554 28.483834662862957 22.19410571604975 42-43 22.326361148619462 26.5870885636044 28.390628079068787 22.69592220870735 44-45 22.262793677086233 26.927349901972836 28.558628654989683 22.25122776595125 46-47 22.71032689521134 27.10682798128145 27.821108957898467 22.361736165608743 48-49 22.362110437832232 27.237850607366504 27.745654266755942 22.654384688045326 50-51 22.609963501606856 27.36703290161417 27.775985733240454 22.247017863538524 52-53 22.89259185084504 26.930997454056378 27.86824701315097 22.30816368194761 54-55 22.224605671293038 27.250943862009297 28.055584404995663 22.468866061702 56-57 22.424125950654993 27.333455307062394 27.772884269417 22.469534472865618 58-59 22.34206546274117 26.897771269469988 27.88063934633352 22.879523921455323 60-61 22.016332552511354 27.20767707618838 28.1421397973314 22.63385057396886 62-63 22.226535841077222 27.094497855133902 28.289408022134044 22.389558281654832 64-65 21.91834448192185 27.172494608770037 28.132716705929777 22.776444203378336 66-67 21.829210078536466 27.23819870335811 28.078772313481355 22.853818904624077 68-69 22.472064065805146 26.812479010438 28.171162881589428 22.544294042167422 70-71 22.277140258280337 26.894607526688613 27.721015298202833 23.107236916828217 72-73 22.206476684776103 27.045449397664434 27.809880050607187 22.938193866952275 74-75 22.321685843119752 27.325899609621917 27.674229011778323 22.67818553548001 76-77 22.375611731328878 27.008778388046395 27.75735909694697 22.85825078367776 78-79 22.452753275282234 27.218594253232414 27.63264104459625 22.6960114268891 80-81 22.694155734176583 27.22526097968505 27.39791287428603 22.682670411852335 82-83 22.57350565872371 27.31097106361488 27.538442649482658 22.57708062817875 84-85 22.567362369601195 27.425059055458828 27.479573031014247 22.528005543925726 86-87 22.721896152157548 27.379726411739945 27.177371007124577 22.721006428977926 88-89 22.54070734688449 27.40800092681075 27.062841587525853 22.98845013877891 90-91 22.576906708695464 27.461459669994127 27.08538501494363 22.87624860636678 92-93 22.803217135968502 26.98814404726137 27.39207384443736 22.816564972332767 94-95 22.507789006316152 26.91050510623873 27.49825450209015 23.08345138535497 96-97 22.896243452826614 26.460944631168147 27.493654082059148 23.149157833946095 98-99 22.641115948807748 26.765851402159928 27.05581119252557 23.53722145650676 100-101 23.20348179686398 26.1512122494519 27.4303252742362 23.214980679447915 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 279.0 1 205.5 2 196.0 3 263.5 4 232.5 5 241.5 6 405.5 7 836.5 8 1401.5 9 1634.0 10 1513.5 11 1408.0 12 1476.0 13 1620.0 14 1822.5 15 2041.0 16 2276.0 17 2558.0 18 2849.5 19 3318.5 20 3917.5 21 4828.5 22 6086.0 23 7512.5 24 9697.0 25 12944.0 26 16949.0 27 21618.0 28 26201.5 29 30822.0 30 35954.0 31 43711.5 32 54861.0 33 60026.5 34 61161.0 35 65709.5 36 71858.0 37 78410.0 38 84047.0 39 88624.0 40 93556.5 41 99391.0 42 103507.5 43 107207.5 44 110743.5 45 115249.5 46 117515.0 47 117903.0 48 118650.5 49 116647.0 50 113218.0 51 109583.0 52 106061.5 53 104131.0 54 103429.5 55 97078.5 56 87795.0 57 79901.0 58 74148.5 59 65220.5 60 51980.0 61 43066.0 62 37000.5 63 28516.5 64 19903.0 65 14526.0 66 10769.5 67 8171.0 68 6200.0 69 4689.5 70 3688.5 71 2657.5 72 1912.5 73 1568.5 74 1231.5 75 890.0 76 659.0 77 481.0 78 328.0 79 202.5 80 127.0 81 87.5 82 47.5 83 26.0 84 16.5 85 14.0 86 12.0 87 8.0 88 6.5 89 5.0 90 6.0 91 4.0 92 0.0 93 0.5 94 1.5 95 1.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1878929398451436 2 0.051469984038158306 3 0.04920543414333048 4 0.05619318810451351 5 0.061789861416016584 6 0.06722478116360338 7 0.038529698924856415 8 0.036815111147343905 9 0.04292939586337906 10-11 0.044757211135632954 12-13 0.040939827027208886 14-15 0.03618427224807044 16-17 0.03401677449159238 18-19 0.04241178445884698 20-21 0.05255373291639735 22-23 0.04967451947868768 24-25 0.07117156812316046 26-27 0.055238842077407486 28-29 0.04653650033871196 30-31 0.055999083827813975 32-33 0.05224640114495642 34-35 0.05143763332537506 36-37 0.04894662844106444 38-39 0.027740736211640953 40-41 0.026074674503303332 42-43 0.022613148235495074 44-45 0.004593801215222175 46-47 0.012665304054644236 48-49 0.01166243195836334 50-51 0.00973756454775968 52-53 0.010449280228991284 54-55 0.011743308740321475 56-57 0.009462583489102016 58-59 0.012018289798979141 60-61 0.01171095802753822 62-63 0.004415872294914274 64-65 0.007198033594274183 66-67 0.004448223007697529 68-69 0.009705213834976426 70-71 0.024554191002490358 72-73 0.021092664734682097 74-75 0.021238242942206748 76-77 0.013150564746393058 78-79 0.005402569034803543 80-81 0.007133332168707673 82-83 0.006033407934077011 84-85 0.00554814724232819 86-87 0.008831744589828548 88-89 0.030894930708008292 90-91 0.038076788945890845 92-93 0.023729247826517363 94-95 0.03330505881036077 96-97 0.036993040067651806 98-99 0.02243521931518717 100-101 0.12505168026367125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3091122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.211546765871866 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87766255954864 39.31144696240723 2 13.965370429019746 14.862379233730163 3 5.2431543371841745 8.369890566412789 4 2.4112291546730025 5.132209317084646 5 1.2930981923315457 3.44038774670572 6 0.781740617769355 2.495857646472936 7 0.5129054391904748 1.9104744234767799 8 0.3517921331905164 1.4975522837706388 9 0.25199178741919087 1.2067985502784724 >10 1.201412406253334 11.242196103398403 >50 0.07034084120792754 2.5666575229096122 >100 0.03374792215819899 3.4152990974902813 >500 0.0025634677177122224 0.9538032918738131 >1k 0.0028686424448456584 3.1786514489838624 >5k 1.2206989127002804E-4 0.41639580500472145 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7195 0.23276337847551795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5626 0.18200511011859125 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4616 0.14933089020750395 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4314 0.139560974946961 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4158 0.13451426375277326 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3929 0.1271059505254079 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3917 0.12671774197200888 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3325 0.10756612000432206 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3318 0.10733966501483927 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3316 0.10727496358927276 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.2350712783254756E-5 7 0.0 0.0 0.0 0.0 3.2350712783254756E-5 8 0.0 0.0 0.0 0.0 3.2350712783254756E-5 9 0.0 0.0 0.0 3.2350712783254756E-5 3.2350712783254756E-5 10-11 0.0 0.0 0.0 3.2350712783254756E-5 3.2350712783254756E-5 12-13 0.0 0.0 0.0 3.2350712783254756E-5 6.470142556650951E-5 14-15 1.6175356391627378E-5 0.0 0.0 3.2350712783254756E-5 9.705213834976427E-5 16-17 3.2350712783254756E-5 0.0 0.0 8.087678195813688E-5 1.2940285113301902E-4 18-19 3.2350712783254756E-5 0.0 0.0 1.1322749474139163E-4 1.2940285113301902E-4 20-21 3.2350712783254756E-5 0.0 0.0 2.102796330911559E-4 1.2940285113301902E-4 22-23 3.2350712783254756E-5 0.0 0.0 3.0733177144092014E-4 1.6175356391627378E-4 24-25 3.2350712783254756E-5 0.0 0.0 5.014360481404487E-4 1.6175356391627378E-4 26-27 3.2350712783254756E-5 0.0 0.0 0.0010513981654557795 1.941042766995285E-4 28-29 3.2350712783254756E-5 0.0 0.0 0.0037203319700742964 1.941042766995285E-4 30-31 3.2350712783254756E-5 0.0 0.0 0.012390322995986571 1.941042766995285E-4 32-33 3.2350712783254756E-5 0.0 0.0 0.03079787856965853 1.941042766995285E-4 34-35 3.2350712783254756E-5 0.0 0.0 0.059266505818922705 1.941042766995285E-4 36-37 3.2350712783254756E-5 0.0 0.0 0.10900572672317689 1.941042766995285E-4 38-39 3.2350712783254756E-5 0.0 0.0 0.18481962213073438 1.941042766995285E-4 40-41 3.2350712783254756E-5 0.0 0.0 0.26275248922559513 1.941042766995285E-4 42-43 3.2350712783254756E-5 0.0 0.0 0.3448100722003208 1.941042766995285E-4 44-45 3.2350712783254756E-5 0.0 0.0 0.4486882109473518 2.102796330911559E-4 46-47 3.2350712783254756E-5 0.0 0.0 0.568709355373227 2.2645498948278326E-4 48-49 3.2350712783254756E-5 0.0 0.0 0.6952006423557531 2.4263034587441065E-4 50-51 3.2350712783254756E-5 0.0 0.0 0.8389510346081455 3.558578406158023E-4 52-53 3.2350712783254756E-5 0.0 0.0 0.9734814737173103 3.88208553399057E-4 54-55 3.2350712783254756E-5 0.0 0.0 1.122618259648115 3.88208553399057E-4 56-57 3.2350712783254756E-5 0.0 0.0 1.2978135447258308 3.88208553399057E-4 58-59 3.2350712783254756E-5 0.0 0.0 1.4848491906822183 3.88208553399057E-4 60-61 3.2350712783254756E-5 0.0 0.0 1.6929451506605044 4.205592661823118E-4 62-63 3.2350712783254756E-5 0.0 0.0 1.8943768638054403 4.852606917488213E-4 64-65 3.2350712783254756E-5 0.0 0.0 2.1071960278500814 4.852606917488213E-4 66-67 3.2350712783254756E-5 0.0 0.0 2.360065374320392 4.852606917488213E-4 68-69 6.470142556650951E-5 0.0 0.0 2.631083470662109 4.852606917488213E-4 70-71 6.470142556650951E-5 0.0 0.0 2.917290226655564 4.852606917488213E-4 72-73 6.470142556650951E-5 0.0 0.0 3.220092898306828 5.014360481404487E-4 74-75 6.470142556650951E-5 3.2350712783254756E-5 0.0 3.5217471196542873 5.176114045320761E-4 76-77 6.470142556650951E-5 3.2350712783254756E-5 0.0 3.845027792497352 5.176114045320761E-4 78-79 9.705213834976425E-5 3.2350712783254756E-5 0.0 4.210218813751124 5.499621173153308E-4 80-81 9.705213834976425E-5 3.2350712783254756E-5 0.0 4.588075139059539 5.499621173153308E-4 82-83 9.705213834976425E-5 3.2350712783254756E-5 0.0 4.986846200182329 5.499621173153308E-4 84-85 9.705213834976425E-5 3.2350712783254756E-5 0.0 5.399382489594393 5.499621173153308E-4 86-87 9.705213834976425E-5 3.2350712783254756E-5 0.0 5.82099315394216 5.661374737069582E-4 88-89 9.705213834976425E-5 3.2350712783254756E-5 0.0 6.262580383433588 5.823128300985855E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4340 0.0 49.573177 1 GTATCAA 7990 0.0 43.190605 1 ATCAACG 10000 0.0 33.9793 3 TATCAAC 10290 0.0 33.206406 2 TCAACGC 10350 0.0 32.69249 4 CAACGCA 10570 0.0 32.00945 5 AACGCAG 11120 0.0 30.378105 6 ACGCAGA 12515 0.0 26.83708 7 CGCAGAG 12620 0.0 26.275425 8 TGGTATC 2155 0.0 23.816881 2 GTACATG 9585 0.0 23.09186 1 TACATGG 9410 0.0 22.928446 2 GCAGAGT 14220 0.0 22.717262 9 ACATGGG 9175 0.0 22.324389 3 TAAGGTG 1990 0.0 21.73009 5 GTGGTAT 2695 0.0 21.547611 1 AGAGTAC 12660 0.0 20.675238 10-11 GAGTACT 8670 0.0 20.189537 12-13 CAGAGTA 13230 0.0 19.999907 10-11 GTGTAAG 1525 0.0 19.663858 1 >>END_MODULE