FastQCFastQC Report
Thu 26 May 2016
SRR938418_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938418_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091122
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71310.23069293285738965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60050.1942660302634448No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45760.14803686169617375No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA43990.14231078553353765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.13053512608043294No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA39680.12836762832395487No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA39030.1262648319930433No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38320.12396793138543222No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38280.12383852853429919No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.055.939711
GTATCAA76100.041.6851731
ATCAACG98200.031.7546843
TCAACGC100000.031.1825924
TATCAAC101000.030.9845352
CAACGCA102600.030.2997325
AACGCAG107800.028.9024756
ACGCAGA121850.025.3749647
CGCAGAG123900.024.9934528
TACATGG89150.024.0600032
GTACATG92750.023.7414671
TAAGGTG21050.022.8074995
TGGTATC17550.022.7636892
ACATGGG88600.022.533623
GCAGAGT145550.020.6557759
GAGTACT89000.020.30554612-13
AGAGTAC127850.020.2277310-11
GTAAGGT20700.019.9782434
GTGTAGC22500.019.8694481
CAGAGTA134350.019.56725710-11
Produced by FastQC (version 0.11.5)