##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938418_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3091122 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.803476536998538 31.0 31.0 34.0 30.0 34.0 2 32.03182986630745 31.0 31.0 34.0 30.0 34.0 3 32.09850403833948 33.0 31.0 34.0 30.0 34.0 4 35.76257908940508 37.0 35.0 37.0 35.0 37.0 5 35.63149691277148 37.0 35.0 37.0 33.0 37.0 6 35.659855871104405 37.0 35.0 37.0 33.0 37.0 7 35.601671496628086 37.0 35.0 37.0 33.0 37.0 8 35.58690177870689 37.0 35.0 37.0 33.0 37.0 9 37.18220212595944 39.0 37.0 39.0 33.0 39.0 10-11 37.09282842928879 39.0 37.0 39.0 33.0 39.0 12-13 36.98896306907331 39.0 37.0 39.0 33.0 39.0 14-15 38.059448640331894 40.0 37.0 41.0 33.0 41.0 16-17 37.9770322879524 40.0 37.0 41.0 33.0 41.0 18-19 38.087920664406 40.0 37.5 41.0 33.0 41.0 20-21 38.13942655773535 40.0 37.0 41.0 33.0 41.0 22-23 38.04536411050745 40.0 37.0 41.0 33.0 41.0 24-25 37.94687414472803 40.0 37.0 41.0 33.0 41.0 26-27 37.76471569223085 40.0 37.0 41.0 32.0 41.0 28-29 37.645288668645236 40.0 37.0 41.0 32.0 41.0 30-31 37.41574839168432 39.0 36.5 41.0 31.5 41.0 32-33 37.20801815651404 39.0 36.0 41.0 31.0 41.0 34-35 37.0615003225366 39.0 36.0 41.0 31.0 41.0 36-37 37.05422933808501 39.0 36.0 41.0 31.0 41.0 38-39 36.927497038292245 39.0 36.0 41.0 30.0 41.0 40-41 36.76113074799377 39.0 35.0 41.0 30.0 41.0 42-43 36.55443120653277 39.0 35.0 41.0 30.0 41.0 44-45 36.30888169409036 39.0 35.0 40.0 30.0 41.0 46-47 35.970665667676656 38.5 35.0 40.0 29.0 41.0 48-49 35.63310150812553 38.0 35.0 40.0 28.0 41.0 50-51 35.28253171502128 38.0 34.0 40.0 27.0 41.0 52-53 34.80448280591966 38.0 34.0 40.0 26.0 41.0 54-55 34.499070887528866 38.0 33.0 40.0 25.0 41.0 56-57 34.1740338944888 37.5 33.0 40.0 24.0 41.0 58-59 33.73797216674075 37.0 32.5 40.0 23.0 41.0 60-61 33.36988802124277 36.5 32.0 39.0 22.5 41.0 62-63 32.918122934002604 36.0 31.5 39.0 22.0 40.0 64-65 32.435526485205045 35.5 31.0 39.0 20.5 40.0 66-67 31.95288328962752 35.0 31.0 38.0 18.5 40.0 68-69 31.419632580014635 35.0 30.0 38.0 17.5 40.0 70-71 30.8400239459976 34.0 29.0 37.0 13.5 39.0 72-73 30.390072439716064 34.0 29.0 36.5 11.0 39.0 74-75 29.879137898795324 34.0 29.0 36.0 8.0 39.0 76-77 28.59849303909713 32.5 27.0 35.0 7.0 37.0 78-79 29.30351050524696 33.5 29.0 35.0 7.0 37.0 80-81 29.028429806393923 34.0 28.5 35.0 2.0 37.0 82-83 28.54382858392519 33.0 27.5 35.0 2.0 36.5 84-85 28.02038677218175 33.0 26.5 35.0 2.0 36.0 86-87 27.451929590614668 32.5 26.0 35.0 2.0 35.5 88-89 26.804745978968157 32.0 25.0 34.5 2.0 35.0 90-91 26.254169521617072 32.0 24.5 34.0 2.0 35.0 92-93 25.519136578886243 31.0 22.0 34.0 2.0 35.0 94-95 24.7604936007055 31.0 19.0 34.0 2.0 35.0 96-97 23.7591766031881 31.0 6.5 34.0 2.0 35.0 98-99 22.502858347227964 30.5 2.0 34.0 2.0 35.0 100-101 18.789810625397507 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2856520392143267 1101 2 0.3391657058127251 1101 3 0.28826780377493577 1101 4 0.1518470501197342 1101 5 0.11434726501979497 1101 6 0.10665764575172432 1101 7 0.2062128261947933 1101 8 0.2641302920823776 1101 9 0.28870386837881057 1101 10-11 0.3205027866911436 1101 12-13 0.3450207590214234 1101 14-15 0.47622442098469264 1101 16-17 0.39938737539775815 1101 18-19 0.4333987633876646 1101 20-21 0.4037918451777429 1101 22-23 0.4386471838885768 1101 24-25 0.47828495727912923 1101 26-27 0.47719051979211713 1101 28-29 0.4731892483661184 1101 30-31 0.44013598952484045 1101 32-33 0.4320482741311906 1101 34-35 0.37608055600172463 1101 36-37 0.45460422727673233 1101 38-39 0.5202647019346571 1101 40-41 0.5233101326289571 1101 42-43 0.5546960792109914 1101 44-45 0.4735134684438762 1101 46-47 0.4714693501955267 1101 48-49 0.4923535116335387 1101 50-51 0.47005208140195265 1101 52-53 0.6210999411033882 1101 54-55 0.5303746782428647 1101 56-57 0.49922933543113146 1101 58-59 0.5006002464875436 1101 60-61 0.5555126906867045 1101 62-63 0.5384719116735681 1101 64-65 0.5501130683567226 1101 66-67 0.46589505287260025 1101 68-69 0.5249808162509204 1101 70-71 0.506210593422896 1101 72-73 0.4051497479862931 1101 74-75 0.4833774973670302 1101 76-77 0.39928001789932566 1101 78-79 0.40819886166909214 1101 80-81 0.3757846158342453 1101 82-83 0.3645121453656124 1101 84-85 0.31046028459050845 1101 86-87 0.4018919738368574 1101 88-89 0.25644532243866536 1101 90-91 0.4707336463101832 1101 92-93 0.4642319267625865 1101 94-95 0.5261134328332666 1101 96-97 0.7174691543243057 1101 98-99 0.6654959503869016 1101 100-101 0.5509158525025342 1102 1 0.27222681468670373 1102 2 0.246002947228547 1102 3 0.2836458029162614 1102 4 0.21506112738173755 1102 5 0.13517711213149397 1102 6 0.18580097062241663 1102 7 0.26219029245452674 1102 8 0.2316479781613694 1102 9 0.30796127064608925 1102 10-11 0.3489017910935459 1102 12-13 0.3134623966431036 1102 14-15 0.43083123574979254 1102 16-17 0.43882186949895896 1102 18-19 0.3587492436226327 1102 20-21 0.42076987536420063 1102 22-23 0.33333577475816867 1102 24-25 0.41555024016442843 1102 26-27 0.36811320620085297 1102 28-29 0.2991037899143265 1102 30-31 0.3293416603755688 1102 32-33 0.3729609112490522 1102 34-35 0.3258273721934657 1102 36-37 0.38138572204594823 1102 38-39 0.37787040006203654 1102 40-41 0.4778013132546377 1102 42-43 0.45751046387362493 1102 44-45 0.5752903535495264 1102 46-47 0.3634367815205124 1102 48-49 0.3910940079877605 1102 50-51 0.47472735696113233 1102 52-53 0.3957909141932774 1102 54-55 0.3467541699730674 1102 56-57 0.30223362477354954 1102 58-59 0.4331664764736374 1102 60-61 0.4542480278067629 1102 62-63 0.5132948490005376 1102 64-65 0.4891702878525308 1102 66-67 0.42610580534466536 1102 68-69 0.4467909402833925 1102 70-71 0.3596422468049063 1102 72-73 0.202550211779009 1102 74-75 0.1860009230715889 1102 76-77 0.226676269065198 1102 78-79 0.39112146014653604 1102 80-81 0.31785280924541226 1102 82-83 0.2586583437054131 1102 84-85 0.15437202938517558 1102 86-87 0.1762436712024673 1102 88-89 0.4294840171434515 1102 90-91 0.17034890203099096 1102 92-93 0.4578149610472657 1102 94-95 0.4737855639513917 1102 96-97 0.2802289946662029 1102 98-99 0.12522790349798996 1102 100-101 -0.18313471158121075 1103 1 0.23367540709823942 1103 2 0.24454824850940327 1103 3 0.2696529230845712 1103 4 0.18195999989565337 1103 5 0.23878539694890577 1103 6 0.20613737832437806 1103 7 0.20142229520325117 1103 8 0.11674524959430954 1103 9 0.23897801982059974 1103 10-11 0.34285267448309753 1103 12-13 0.3193273671329848 1103 14-15 0.5124584821655631 1103 16-17 0.4138552160804281 1103 18-19 0.3738380988546268 1103 20-21 0.36805635153958605 1103 22-23 0.45790467255628897 1103 24-25 0.4299967536719649 1103 26-27 0.45581260140046 1103 28-29 0.45672007346063737 1103 30-31 0.6052591976287189 1103 32-33 0.6157762083507379 1103 34-35 0.5069385866588263 1103 36-37 0.5807630077388097 1103 38-39 0.619364852089916 1103 40-41 0.5743833387239974 1103 42-43 0.786340692054587 1103 44-45 0.7701102731082656 1103 46-47 0.650254545178818 1103 48-49 0.7672197471869993 1103 50-51 0.7981777367583405 1103 52-53 0.9831347015371037 1103 54-55 0.77411636970362 1103 56-57 0.8914746178585489 1103 58-59 1.0294050221398692 1103 60-61 0.9493449565387593 1103 62-63 0.9603370951247854 1103 64-65 0.8996312117119487 1103 66-67 0.851511605535773 1103 68-69 0.7726766108000298 1103 70-71 0.68155614697746 1103 72-73 0.6286874781916048 1103 74-75 0.7856269784903382 1103 76-77 0.7048139520597125 1103 78-79 0.856010970248871 1103 80-81 0.7248214102734245 1103 82-83 0.8305310752588646 1103 84-85 0.6118191432869544 1103 86-87 0.7377880863566126 1103 88-89 0.8626276261937846 1103 90-91 0.8608210816134019 1103 92-93 0.6505602047365642 1103 94-95 0.6679476757368761 1103 96-97 0.4507094702816943 1103 98-99 0.5226293625138503 1103 100-101 0.3201304049793876 1104 1 0.35293379234184385 1104 2 0.32226547851342247 1104 3 0.29797380193178924 1104 4 0.1939988626683089 1104 5 0.12650249049656992 1104 6 0.15106998593727639 1104 7 0.22779035554425775 1104 8 0.23478448250485684 1104 9 0.1840703240227768 1104 10-11 0.3514357798205836 1104 12-13 0.32602087163945725 1104 14-15 0.47270650440113116 1104 16-17 0.3691107476803168 1104 18-19 0.373580963892465 1104 20-21 0.31442836058664625 1104 22-23 0.37674842234307704 1104 24-25 0.4818119004990109 1104 26-27 0.4717267183230973 1104 28-29 0.48165139753084674 1104 30-31 0.4823294965363871 1104 32-33 0.5962038881831688 1104 34-35 0.5076582873759108 1104 36-37 0.5136905468506114 1104 38-39 0.4114796570937642 1104 40-41 0.5654786014707227 1104 42-43 0.5962175316569542 1104 44-45 0.4928359506141149 1104 46-47 0.4368986226314533 1104 48-49 0.4497264036990458 1104 50-51 0.5058814874320845 1104 52-53 0.5432745394610592 1104 54-55 0.5244047403805183 1104 56-57 0.4685393092624821 1104 58-59 0.4581428178237559 1104 60-61 0.41048580850407745 1104 62-63 0.4781856942296372 1104 64-65 0.46389160542267405 1104 66-67 0.2810035358475069 1104 68-69 0.32945205308922354 1104 70-71 0.2748933664743447 1104 72-73 0.13211882273028053 1104 74-75 0.3061220848239614 1104 76-77 0.1781177568043013 1104 78-79 0.12043238246990384 1104 80-81 0.25349153212895814 1104 82-83 0.14898078597137498 1104 84-85 0.2852896166881962 1104 86-87 0.30978331720666574 1104 88-89 0.23755289501282206 1104 90-91 0.3610430364190549 1104 92-93 0.3185718905184949 1104 94-95 0.3126529342534603 1104 96-97 0.07283503828131188 1104 98-99 0.07408513871176226 1104 100-101 -0.29194771031941613 1105 1 0.16038086309588095 1105 2 0.22383822466147762 1105 3 0.13222639929274038 1105 4 0.14938838664579634 1105 5 0.1164376165968406 1105 6 0.1666527487597449 1105 7 0.15610259200030185 1105 8 0.18903595754935054 1105 9 0.2143918075648088 1105 10-11 0.2449063029803895 1105 12-13 0.23206639960918807 1105 14-15 0.35039309953347697 1105 16-17 0.26893939400633116 1105 18-19 0.27136098184952573 1105 20-21 0.10068910275848708 1105 22-23 0.2564616003424689 1105 24-25 0.3536264757823915 1105 26-27 0.2259647155493738 1105 28-29 0.18343993908060696 1105 30-31 0.36522679656363977 1105 32-33 0.35347780154033615 1105 34-35 0.34890707487330275 1105 36-37 0.2792562244090959 1105 38-39 0.24199619322433819 1105 40-41 0.20193870512466816 1105 42-43 0.1772921697098937 1105 44-45 0.22827594877031032 1105 46-47 0.030148490358435254 1105 48-49 -0.03061006076360684 1105 50-51 -0.2580677538018179 1105 52-53 -0.359246313579618 1105 54-55 -0.15135207167957532 1105 56-57 -0.07196353861553462 1105 58-59 0.007811534054056324 1105 60-61 -0.2362387309453453 1105 62-63 0.019630786900904695 1105 64-65 0.026375522226956605 1105 66-67 -0.1230395089970493 1105 68-69 -0.25141936551878175 1105 70-71 -0.21985790013994588 1105 72-73 -0.14913703936909073 1105 74-75 -0.07332479353296861 1105 76-77 -0.06638599894757036 1105 78-79 0.07580562283802195 1105 80-81 -0.012185124490304844 1105 82-83 0.033208331625605325 1105 84-85 0.10922005912137678 1105 86-87 0.14118613261767265 1105 88-89 0.020948874314100863 1105 90-91 0.12387902990962019 1105 92-93 0.13398620566495723 1105 94-95 0.2216967506905867 1105 96-97 0.09793098631806174 1105 98-99 -0.04643631757400968 1105 100-101 -0.05631711877847678 1106 1 0.07894111192947406 1106 2 0.11251754036909745 1106 3 0.1330774101792116 1106 4 0.10851597607037178 1106 5 0.06603537709450791 1106 6 0.03330730552490735 1106 7 0.14059179884322504 1106 8 0.15503035879351756 1106 9 0.10897743742484067 1106 10-11 0.07803949116079423 1106 12-13 0.16850783040234774 1106 14-15 0.23724995800937165 1106 16-17 -0.01200368530937368 1106 18-19 0.31480508760380843 1106 20-21 0.1385909379062369 1106 22-23 0.2136992986317381 1106 24-25 0.14062865307633388 1106 26-27 0.13709429564268305 1106 28-29 0.27265897485043666 1106 30-31 0.24110660993844846 1106 32-33 0.21354399127594803 1106 34-35 -0.028819083758115482 1106 36-37 -0.3392559995411446 1106 38-39 -0.01839472434953393 1106 40-41 0.20767133342948796 1106 42-43 0.2458269131002524 1106 44-45 0.19515445576875123 1106 46-47 -0.07370818149225755 1106 48-49 -0.02532632825972314 1106 50-51 -0.09135248164007947 1106 52-53 0.019116859048686763 1106 54-55 -0.2310106719906173 1106 56-57 -0.010332185582875297 1106 58-59 -0.07859841928964073 1106 60-61 -0.4512745007742751 1106 62-63 -0.37972373876005605 1106 64-65 -0.5237607141649576 1106 66-67 -0.3519606909566164 1106 68-69 -0.9102237201066536 1106 70-71 -0.6672417881648336 1106 72-73 -0.4494177236614725 1106 74-75 -0.24387533785645132 1106 76-77 -0.17146543907199074 1106 78-79 -0.37779313299401807 1106 80-81 -0.919425684365887 1106 82-83 -0.48010426557501873 1106 84-85 -0.33718342454891825 1106 86-87 -0.14473585805106737 1106 88-89 -0.14261292972944517 1106 90-91 -0.072603458426304 1106 92-93 -0.2557857383474875 1106 94-95 -0.03053814822076717 1106 96-97 -0.13041439470838156 1106 98-99 -0.37112857262843946 1106 100-101 -0.5305650814534388 1107 1 0.10694407579221377 1107 2 0.13545686621178987 1107 3 0.14470595557853727 1107 4 0.09099060710590123 1107 5 0.0595004970459172 1107 6 0.1042812259621968 1107 7 0.07611728927748374 1107 8 0.06348635636412325 1107 9 0.07778807817795297 1107 10-11 0.12405326583434118 1107 12-13 0.1409148027679663 1107 14-15 0.26295388755690396 1107 16-17 0.20031529434444195 1107 18-19 0.2411571799208403 1107 20-21 0.2391391067492279 1107 22-23 0.2037278926189856 1107 24-25 0.25351586838152684 1107 26-27 0.2682536275588703 1107 28-29 0.21960709510552334 1107 30-31 0.19432655527705123 1107 32-33 0.21666863142171877 1107 34-35 0.19064460284984364 1107 36-37 0.14104505117249744 1107 38-39 0.22510948457849622 1107 40-41 0.19823614363294695 1107 42-43 0.31570245515916184 1107 44-45 0.34926108194549244 1107 46-47 0.23637740611781766 1107 48-49 0.3038539207533404 1107 50-51 0.27927797083486894 1107 52-53 0.3185752495740459 1107 54-55 0.32548021170206454 1107 56-57 0.3992223361298528 1107 58-59 0.1905001534403894 1107 60-61 0.024664764333529376 1107 62-63 0.20117299977623304 1107 64-65 0.22513621872299439 1107 66-67 0.168911559271141 1107 68-69 0.2701197964436055 1107 70-71 0.08675148240911312 1107 72-73 0.1564992152999629 1107 74-75 0.2753970581350451 1107 76-77 0.3404234403692499 1107 78-79 0.3405682396145444 1107 80-81 0.556013028054398 1107 82-83 0.5105000466023277 1107 84-85 0.5392968183906497 1107 86-87 0.5658258911138283 1107 88-89 0.5197685582754126 1107 90-91 0.5065615096878737 1107 92-93 0.3370024062015595 1107 94-95 0.28053960458822047 1107 96-97 0.3349394103326162 1107 98-99 0.3857657851000731 1107 100-101 0.05678792188697912 1108 1 0.23975190947548697 1108 2 0.2475923869948602 1108 3 0.2229228089522124 1108 4 0.1379967122666912 1108 5 0.1330797942724189 1108 6 0.10617798037153392 1108 7 0.1333221055916951 1108 8 0.13386226063401097 1108 9 0.24022308159661776 1108 10-11 0.24927114760250646 1108 12-13 0.2517934745741286 1108 14-15 0.4377508169050799 1108 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-0.5118596474302137 2316 64-65 -0.28006086843832634 2316 66-67 -0.22106533767059133 2316 68-69 -0.31149048962223347 2316 70-71 -0.3008344817779154 2316 72-73 -0.2559497323007811 2316 74-75 0.010634998459501332 2316 76-77 -0.023845272456746613 2316 78-79 -0.08542411108719605 2316 80-81 -0.253615082699735 2316 82-83 -0.25016906039143194 2316 84-85 -0.3708022829259612 2316 86-87 0.05729625549413697 2316 88-89 0.1893756170153864 2316 90-91 0.31283032127131705 2316 92-93 0.23077036162165854 2316 94-95 0.11877270645371496 2316 96-97 0.25374387742984794 2316 98-99 0.4470576168192615 2316 100-101 0.5856611697036129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 17.0 4 44.0 5 87.0 6 186.0 7 500.0 8 1274.0 9 2188.0 10 3767.0 11 5844.0 12 8196.0 13 11533.0 14 15116.0 15 19609.0 16 23875.0 17 29493.0 18 28073.0 19 25394.0 20 25344.0 21 26864.0 22 29305.0 23 33326.0 24 38030.0 25 45251.0 26 54025.0 27 64870.0 28 78203.0 29 93581.0 30 111293.0 31 133352.0 32 160898.0 33 198326.0 34 252491.0 35 331638.0 36 441918.0 37 514710.0 38 270140.0 39 12354.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.893519493539685 19.41065659129223 15.078122649502557 23.617701265665538 2 15.79886358670937 20.401029925118426 39.48053839643054 24.319568091741665 3 19.188241303798563 23.561583919722842 30.3068398827469 26.943334893731695 4 13.173436700330818 17.05316063228821 36.78686897508413 32.986533692296845 5 13.616529940344668 37.433104693421335 34.70351067605058 14.246854690183422 6 32.80595201354071 36.50997922437225 16.897424300949623 13.786644461137412 7 28.787799381583774 32.59421659837431 20.32841149589049 18.289572524151424 8 26.411607176940933 35.60794429983676 19.482116849480544 18.498331673741767 9 26.00337353232904 16.684233103707975 19.85787037845805 37.45452298550494 10-11 24.86192715784107 26.528555003652393 27.675371596462384 20.934146242044154 12-13 25.798075909006503 23.61723348350534 27.86148524710445 22.723205360383705 14-15 22.262482684280982 25.441085793443285 25.884630241058098 26.411801281217627 16-17 22.108800623204132 28.56504854871467 26.92828041080229 22.397870417278902 18-19 22.184404238978598 27.021854847527855 28.956120140195047 21.837620773298497 20-21 22.481755815525883 26.32764090191199 29.31089423193261 21.87970905062951 22-23 22.22599108026147 26.280279458397303 29.147895165574184 22.345834295767038 24-25 22.190185926617996 26.49861926711164 29.21264257005246 22.098552236217905 26-27 21.81134465961621 26.908851376184785 28.87914188395219 22.400662080246818 28-29 21.973170259860336 27.288117389090434 28.696440968683863 22.042271382365367 30-31 22.151827071205858 26.779014222020354 28.671725024117457 22.397433682656327 32-33 21.731251629667156 26.783122762543826 28.709801813063347 22.775823794725667 34-35 22.50705033289801 26.981279835408923 28.499910136868582 22.01175969482448 36-37 22.67872896514175 26.461630885126063 28.545908455805165 22.31373169392702 38-39 22.093846347957463 26.81604071161206 28.525223147126667 22.56488979330381 40-41 22.695911499397276 26.6302206238705 28.544915789970887 22.128952086761338 42-43 22.32651038969925 26.699156566345245 28.400242048620328 22.574090995335176 44-45 22.25070702482788 26.943452895097636 28.617117020939318 22.188723059135164 46-47 22.70984451600422 27.089419311175682 27.89766951935252 22.303066653467578 48-49 22.37208689919065 27.300070977463847 27.784102342126904 22.5437397812186 50-51 22.644512251538437 27.322360618571505 27.82788903187904 22.205238098011012 52-53 22.937528832572767 26.94028252524488 27.86090293427435 22.261285707908 54-55 22.242522294493714 27.26899811783553 28.051966243972252 22.436513343698504 56-57 22.39298545964863 27.363931284497987 27.770482692045157 22.472600563808225 58-59 22.30470036446313 27.02567223163628 27.83403566730786 22.835591736592733 60-61 22.009354532108404 27.254634401359766 28.103597981574328 22.632413084957502 62-63 22.202698567057528 27.210378626272274 28.17104921772741 22.415873588942787 64-65 21.86429393598829 27.22584226698267 28.044574106101276 22.86528969092776 66-67 21.778368358394367 27.2733992856958 27.99721460182717 22.951017754082663 68-69 22.415307746769464 26.846594654277933 28.08433016395357 22.653767434999033 70-71 22.18167954415206 26.950729970397447 27.66325522622313 23.204335259227364 72-73 22.128081648022953 27.08105665192121 27.74531383749978 23.045547862556056 74-75 22.172175669546526 27.40050376529946 27.525636322345093 22.901684242808923 76-77 22.124005458212263 27.042138744442955 27.85550036523955 22.978355432105236 78-79 22.261981248232843 27.21870893481396 27.682796085046142 22.836513731907054 80-81 22.436385088122126 27.29491428479921 27.42904218413017 22.83965844294849 82-83 22.322608947004813 27.35631618696442 27.538500434230322 22.782574431800445 84-85 22.231719459099647 27.471859448083645 27.467022759128845 22.829398333687863 86-87 22.41184476616868 27.460900864527392 27.161035414145278 22.966218955158645 88-89 22.173347468442678 27.506671527902014 27.03326721923859 23.286713784416722 90-91 22.1349102862174 27.475243240550178 27.198312404188318 23.1915340690441 92-93 22.229544323732213 27.136732643222572 27.44024938711099 23.19347364593423 94-95 21.900255295691224 27.02574130457182 27.54566586916755 23.528337530569406 96-97 22.213293425494044 26.60990087094589 27.567045234707656 23.60976046885241 98-99 21.906829578465157 26.95770029023456 27.153729529469977 23.981740601830307 100-101 21.982794596913354 26.60244403164935 27.590289221842422 23.82447214959487 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 331.0 1 223.0 2 179.5 3 253.0 4 219.5 5 209.0 6 381.0 7 840.0 8 1372.0 9 1585.0 10 1425.0 11 1229.5 12 1364.0 13 1588.5 14 1772.0 15 1972.5 16 2183.5 17 2547.0 18 2890.5 19 3314.5 20 3911.5 21 4724.5 22 5937.5 23 7384.0 24 9547.0 25 12729.5 26 16668.0 27 21468.5 28 26061.5 29 30860.0 30 36221.5 31 43616.5 32 55478.5 33 60758.0 34 61125.0 35 65869.0 36 72283.0 37 78797.0 38 83984.5 39 88685.5 40 93588.5 41 99259.5 42 104201.5 43 108133.0 44 111887.5 45 116623.5 46 118721.5 47 119745.5 48 120005.5 49 116915.5 50 113812.5 51 110670.5 52 106773.5 53 105556.0 54 105753.5 55 98205.5 56 87985.5 57 79485.5 58 72929.0 59 63821.0 60 50497.5 61 41798.5 62 36250.0 63 27253.0 64 18015.0 65 12903.5 66 9628.5 67 7472.0 68 5525.0 69 4059.0 70 3165.0 71 2218.0 72 1622.0 73 1393.5 74 1089.5 75 789.0 76 571.5 77 387.0 78 236.0 79 140.0 80 96.0 81 60.0 82 27.5 83 13.0 84 4.5 85 2.5 86 3.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.793649684483498E-4 2 0.051017074059192744 3 0.0010028720962808975 4 0.0 5 0.0877998344937534 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.2350712783254756E-4 26-27 0.008346483898079727 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010044896319200601 36-37 9.705213834976427E-5 38-39 0.0036232798317245326 40-41 0.005176114045320761 42-43 2.4263034587441065E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.470142556650951E-5 68-69 0.0010837488782390343 70-71 1.1322749474139165E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001374905293288327 82-83 6.631896120567225E-4 84-85 0.005321692252845406 86-87 0.002232199182044578 88-89 3.2350712783254756E-5 90-91 0.0031541944963673386 92-93 7.764171067981141E-4 94-95 1.6175356391627378E-5 96-97 0.0 98-99 4.8526069174882134E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3091122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.61071144845863 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48786095437126 36.18051502829216 2 15.186457172443104 15.371948037577862 3 5.7128200034911 8.673896542610114 4 2.584737251915197 5.232615649070479 5 1.4215688788226915 3.5973306165102055 6 0.895283702850533 2.7186567089685547 7 0.5478207628476619 1.9407918987770252 8 0.3892619805112096 1.5760660618806686 9 0.28199310328282595 1.2844684422632249 >10 1.3647418272055716 12.091755959389856 >50 0.08238309615545693 2.8439825480220797 >100 0.03855344721954826 3.62498817530204 >500 0.003195009257666612 1.0734081691660144 >1k 0.003195009256114649 3.3647534781654636 >5k 1.2780037024458596E-4 0.4248226840043591 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7131 0.23069293285738965 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6005 0.1942660302634448 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4576 0.14803686169617375 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4399 0.14231078553353765 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4035 0.13053512608043294 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3968 0.12836762832395487 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3903 0.1262648319930433 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3832 0.12396793138543222 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3828 0.12383852853429919 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.2350712783254756E-5 0.0 0.0 0.0 0.0 7 3.2350712783254756E-5 0.0 0.0 0.0 0.0 8 3.2350712783254756E-5 0.0 0.0 3.2350712783254756E-5 0.0 9 3.2350712783254756E-5 0.0 0.0 6.470142556650951E-5 0.0 10-11 3.2350712783254756E-5 0.0 0.0 9.705213834976425E-5 0.0 12-13 3.2350712783254756E-5 0.0 0.0 9.705213834976425E-5 4.8526069174882134E-5 14-15 3.2350712783254756E-5 0.0 0.0 9.705213834976425E-5 8.087678195813688E-5 16-17 3.2350712783254756E-5 0.0 0.0 1.455782075246464E-4 1.1322749474139163E-4 18-19 3.2350712783254756E-5 0.0 0.0 1.7792892030790114E-4 1.2940285113301902E-4 20-21 3.2350712783254756E-5 0.0 0.0 2.749810586576654E-4 1.2940285113301902E-4 22-23 3.2350712783254756E-5 0.0 0.0 3.720331970074297E-4 1.6175356391627378E-4 24-25 3.2350712783254756E-5 0.0 0.0 5.661374737069582E-4 1.6175356391627378E-4 26-27 3.2350712783254756E-5 0.0 0.0 0.0010999242346306616 1.6175356391627378E-4 28-29 4.8526069174882134E-5 0.0 0.0 0.003752682682857551 1.6175356391627378E-4 30-31 6.470142556650951E-5 0.0 0.0 0.012438849065161454 1.7792892030790114E-4 32-33 6.470142556650951E-5 0.0 0.0 0.031056684271924564 1.941042766995285E-4 34-35 6.470142556650951E-5 0.0 0.0 0.05962236365953851 2.2645498948278326E-4 36-37 6.470142556650951E-5 0.0 0.0 0.10955568884049222 2.2645498948278326E-4 38-39 6.470142556650951E-5 0.0 0.0 0.18546663638639949 2.2645498948278326E-4 40-41 6.470142556650951E-5 0.0 0.0 0.26323774991734394 2.2645498948278326E-4 42-43 6.470142556650951E-5 0.0 0.0 0.34510122861537007 2.2645498948278326E-4 44-45 6.470142556650951E-5 0.0 0.0 0.44927052377745036 2.2645498948278326E-4 46-47 6.470142556650951E-5 0.0 0.0 0.5693240189161088 2.4263034587441065E-4 48-49 6.470142556650951E-5 0.0 0.0 0.6960579362445093 2.5880570226603805E-4 50-51 6.470142556650951E-5 0.0 0.0 0.8395980488638106 2.5880570226603805E-4 52-53 6.470142556650951E-5 3.2350712783254756E-5 0.0 0.9743872936752416 2.911564150492928E-4 54-55 6.470142556650951E-5 3.2350712783254756E-5 0.0 1.124171093861711 2.911564150492928E-4 56-57 6.470142556650951E-5 3.2350712783254756E-5 0.0 1.2999648671259174 2.911564150492928E-4 58-59 6.470142556650951E-5 3.2350712783254756E-5 0.0 1.4873887216357038 2.911564150492928E-4 60-61 6.470142556650951E-5 3.2350712783254756E-5 0.0 1.694837667358325 2.911564150492928E-4 62-63 6.470142556650951E-5 3.2350712783254756E-5 0.0 1.8966575890566597 2.911564150492928E-4 64-65 6.470142556650951E-5 3.2350712783254756E-5 0.0 2.1098973123674836 2.911564150492928E-4 66-67 6.470142556650951E-5 3.2350712783254756E-5 0.0 2.3628798863325358 2.911564150492928E-4 68-69 6.470142556650951E-5 3.2350712783254756E-5 0.0 2.634836153344967 2.911564150492928E-4 70-71 6.470142556650951E-5 3.2350712783254756E-5 0.0 2.922708971046759 2.911564150492928E-4 72-73 8.087678195813688E-5 3.2350712783254756E-5 0.0 3.2287467139763493 2.911564150492928E-4 74-75 9.705213834976425E-5 3.2350712783254756E-5 0.0 3.534218319432232 2.911564150492928E-4 76-77 9.705213834976425E-5 3.2350712783254756E-5 0.0 3.8632574191507163 2.911564150492928E-4 78-79 9.705213834976425E-5 3.2350712783254756E-5 0.0 4.234999459743097 3.396824842241749E-4 80-81 1.2940285113301902E-4 3.2350712783254756E-5 0.0 4.620652306832277 3.7203319700742965E-4 82-83 1.6175356391627378E-4 3.2350712783254756E-5 0.0 5.028400690752419 4.205592661823118E-4 84-85 1.6175356391627378E-4 3.2350712783254756E-5 0.0 5.45093335041451 4.205592661823118E-4 86-87 1.6175356391627378E-4 3.2350712783254756E-5 0.0 5.883171223911576 4.3673462257393916E-4 88-89 1.6175356391627378E-4 3.2350712783254756E-5 0.0 6.337795790654655 4.529099789655665E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4285 0.0 55.93971 1 GTATCAA 7610 0.0 41.685173 1 ATCAACG 9820 0.0 31.754684 3 TCAACGC 10000 0.0 31.182592 4 TATCAAC 10100 0.0 30.984535 2 CAACGCA 10260 0.0 30.299732 5 AACGCAG 10780 0.0 28.902475 6 ACGCAGA 12185 0.0 25.374964 7 CGCAGAG 12390 0.0 24.993452 8 TACATGG 8915 0.0 24.060003 2 GTACATG 9275 0.0 23.741467 1 TAAGGTG 2105 0.0 22.807499 5 TGGTATC 1755 0.0 22.763689 2 ACATGGG 8860 0.0 22.53362 3 GCAGAGT 14555 0.0 20.655775 9 GAGTACT 8900 0.0 20.305546 12-13 AGAGTAC 12785 0.0 20.22773 10-11 GTAAGGT 2070 0.0 19.978243 4 GTGTAGC 2250 0.0 19.869448 1 CAGAGTA 13435 0.0 19.567257 10-11 >>END_MODULE