FastQCFastQC Report
Thu 26 May 2016
SRR938417_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938417_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085869
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72960.23643258997708586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57760.18717580039852633No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46150.1495526867796397No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA42210.1367848084283552No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41950.1359422580803009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39790.12894260903492663No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38430.12453542259895024No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA33600.10888342959471059No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33490.10852696598591838No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32830.10638818433316515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41850.052.375111
GTATCAA77450.042.38971
ATCAACG97100.033.0889243
TCAACGC99000.032.357874
TATCAAC101750.031.9504432
CAACGCA101350.031.5137965
AACGCAG105500.030.45096
ACGCAGA119050.026.8162387
CGCAGAG121100.026.1269118
GTACATG90000.024.7250371
TACATGG88600.024.6762582
TGGTATC21000.023.9906372
ACATGGG89350.023.3520583
GTGGTAT24550.022.7089421
GCAGAGT138450.022.3366419
TAAGGTG21100.022.0749055
AGAGTAC121200.021.37914510-11
GTAAGGT21000.021.048394
GTGTAGC20600.020.5866341
GTGTAAG14150.020.541681
Produced by FastQC (version 0.11.5)