FastQCFastQC Report
Thu 26 May 2016
SRR938417_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938417_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085869
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72630.23536319915070927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59710.19349492800893364No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA45720.14815923812708834No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44450.14404370373466924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41600.1348080556886893No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40750.1320535641662041No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39080.12664179846908602No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA38290.12408174164230562No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37560.12171611951123007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44600.057.0544621
GTATCAA77700.039.7277831
ATCAACG97800.031.1095263
TCAACGC100750.030.1514384
TATCAAC101700.030.0718862
CAACGCA102650.029.6396645
AACGCAG106850.028.4473846
ACGCAGA121450.024.9493967
CGCAGAG123150.024.4507228
TGGTATC18650.023.2077582
TAAGGTG21050.022.8098015
GTACATG87150.021.5575961
TACATGG84200.021.352582
GTAAGGT21350.021.1532924
GCAGAGT140700.020.894579
GTGTAAG14750.020.6375711
GAGTACT93500.020.52138312-13
CAGAGTA132800.020.15267610-11
ACATGGG85250.020.0752223
AGAGTAC127550.019.8092510-11
Produced by FastQC (version 0.11.5)