##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938417_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3085869 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.814059508034852 31.0 31.0 34.0 30.0 34.0 2 32.04833257665831 33.0 31.0 34.0 30.0 34.0 3 32.11867580898606 33.0 31.0 34.0 30.0 34.0 4 35.782725060590714 37.0 35.0 37.0 35.0 37.0 5 35.65168936205652 37.0 35.0 37.0 33.0 37.0 6 35.690798928924075 37.0 35.0 37.0 33.0 37.0 7 35.627672788443064 37.0 35.0 37.0 33.0 37.0 8 35.60925658218155 37.0 35.0 37.0 33.0 37.0 9 37.194727968037526 39.0 37.0 39.0 33.0 39.0 10-11 37.13128765349404 39.0 37.0 39.0 33.0 39.0 12-13 37.02562357637346 39.0 37.0 39.0 33.0 39.0 14-15 38.08844445438221 40.0 37.0 41.0 33.0 41.0 16-17 38.002474991647404 40.0 37.0 41.0 33.0 41.0 18-19 38.00107182126007 40.0 37.0 41.0 33.0 41.0 20-21 37.9939409287951 40.0 37.0 41.0 33.0 41.0 22-23 38.028903851718916 40.0 37.0 41.0 33.0 41.0 24-25 37.956391700360584 40.0 37.0 41.0 33.0 41.0 26-27 37.780324440214414 40.0 37.0 41.0 32.0 41.0 28-29 37.65512567124528 40.0 37.0 41.0 32.0 41.0 30-31 37.41492720527022 39.0 36.5 41.0 31.5 41.0 32-33 37.19953731023578 39.0 36.0 41.0 31.0 41.0 34-35 37.02895489082654 39.0 36.0 41.0 31.0 41.0 36-37 37.031057215973846 39.0 36.0 41.0 31.0 41.0 38-39 36.88164338797272 39.0 36.0 41.0 30.0 41.0 40-41 36.706523834939205 39.0 35.0 41.0 30.0 41.0 42-43 36.48962788115763 39.0 35.0 40.0 30.0 41.0 44-45 36.22703750548063 39.0 35.0 40.0 30.0 41.0 46-47 35.8902289760194 38.0 35.0 40.0 28.5 41.0 48-49 35.51492464521339 38.0 34.5 40.0 27.5 41.0 50-51 35.15190372630853 38.0 34.0 40.0 26.5 41.0 52-53 34.64087944109099 38.0 33.0 40.0 25.5 41.0 54-55 34.323393993717815 38.0 33.0 40.0 24.0 41.0 56-57 33.96623884552455 37.0 33.0 40.0 24.0 41.0 58-59 33.50800358019086 37.0 32.0 39.5 23.0 41.0 60-61 33.09810721064309 36.0 31.5 39.0 22.0 40.5 62-63 32.62370129775438 36.0 31.0 39.0 20.5 40.0 64-65 32.134352754442915 35.0 31.0 39.0 18.5 40.0 66-67 31.63829281152246 35.0 30.0 38.0 17.5 40.0 68-69 31.075788538009874 34.5 29.5 38.0 13.0 40.0 70-71 30.505788482920046 34.0 29.0 37.0 9.0 39.0 72-73 29.919186459308545 34.0 28.5 36.5 7.5 39.0 74-75 29.30385217259709 34.0 28.0 36.0 6.5 39.0 76-77 28.00874243203454 31.5 26.0 34.5 2.0 36.5 78-79 28.632438220805874 33.0 27.0 35.0 2.0 37.0 80-81 28.32119769180092 33.0 27.0 35.0 2.0 37.0 82-83 27.817684742936265 33.0 26.0 35.0 2.0 36.0 84-85 27.219249747801996 32.5 26.0 35.0 2.0 36.0 86-87 26.573732715160624 32.0 24.5 35.0 2.0 35.5 88-89 25.808226467163706 31.5 22.5 34.0 2.0 35.0 90-91 25.249178108338363 31.0 20.0 34.0 2.0 35.0 92-93 24.474088660276895 31.0 17.5 34.0 2.0 35.0 94-95 23.682101378898455 31.0 5.0 34.0 2.0 35.0 96-97 22.693999648073202 30.0 2.0 34.0 2.0 35.0 98-99 21.506112702775134 30.0 2.0 34.0 2.0 35.0 100-101 18.333020617531076 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32268826688156693 1101 2 0.34156411385917096 1101 3 0.24997673472479676 1101 4 0.2540265240621906 1101 5 0.24240922270412568 1101 6 0.24833479555815785 1101 7 0.2828584754106842 1101 8 0.2934351447747332 1101 9 0.3307563791680508 1101 10-11 0.31872545414511677 1101 12-13 0.4025849729900557 1101 14-15 0.4926466510054368 1101 16-17 0.5461055802536521 1101 18-19 0.5150158993929921 1101 20-21 0.4626554651292025 1101 22-23 0.4738853575205084 1101 24-25 0.5482754041976747 1101 26-27 0.5354536865912252 1101 28-29 0.5748220718148502 1101 30-31 0.46613000360986234 1101 32-33 0.4713392603736821 1101 34-35 0.4201860174462624 1101 36-37 0.6600827724228111 1101 38-39 0.7484910230498798 1101 40-41 0.8105254968851483 1101 42-43 0.718110335019098 1101 44-45 0.6988320955153213 1101 46-47 0.7967600482886752 1101 48-49 0.6946032352105647 1101 50-51 0.821476534458732 1101 52-53 0.8297018878251095 1101 54-55 1.0693250743532374 1101 56-57 1.0438602286381524 1101 58-59 1.2075173005123645 1101 60-61 1.2260622282694698 1101 62-63 1.218969786849577 1101 64-65 1.2011593700550733 1101 66-67 1.251400514801908 1101 68-69 1.2880483677213448 1101 70-71 1.387070954439153 1101 72-73 1.3992319713009138 1101 74-75 1.5226679772457068 1101 76-77 1.309586951684242 1101 78-79 1.6538706918608597 1101 80-81 1.945494423737312 1101 82-83 2.09854630819358 1101 84-85 2.223695483976126 1101 86-87 2.390167521261933 1101 88-89 2.563682613902838 1101 90-91 2.703245489421448 1101 92-93 2.9104783032128623 1101 94-95 2.9952345543748002 1101 96-97 3.3734188018476807 1101 98-99 3.785422437011796 1101 100-101 4.141171006220663 1102 1 0.2945214911478935 1102 2 0.3124927711014891 1102 3 0.3175043031141982 1102 4 0.17518285916130338 1102 5 0.1103896751862905 1102 6 0.05584415711077639 1102 7 0.19989032212953362 1102 8 0.28463594363954314 1102 9 0.27180083069113437 1102 10-11 0.3444634581796251 1102 12-13 0.37684795067409027 1102 14-15 0.4830244232342622 1102 16-17 0.47698681933491827 1102 18-19 0.49873334647200807 1102 20-21 0.5241341457826323 1102 22-23 0.5135620903057188 1102 24-25 0.3983736222514551 1102 26-27 0.4811563926464473 1102 28-29 0.508304749564239 1102 30-31 0.32214197872826134 1102 32-33 0.2981236839882371 1102 34-35 0.44013205526484 1102 36-37 0.55806535234683 1102 38-39 0.6847657083628889 1102 40-41 0.6637504307808442 1102 42-43 0.610699006726172 1102 44-45 0.5948126048216693 1102 46-47 0.5395318855784126 1102 48-49 0.7922633841130136 1102 50-51 0.8239757589010708 1102 52-53 0.873209170906307 1102 54-55 0.8700600581595026 1102 56-57 1.004583113052 1102 58-59 1.0121760401289492 1102 60-61 1.0281391635890031 1102 62-63 1.3412248151542627 1102 64-65 1.2114059130775559 1102 66-67 1.290395934911615 1102 68-69 1.4828973251246964 1102 70-71 1.59069023890261 1102 72-73 1.7227572625652599 1102 74-75 1.6825211514189782 1102 76-77 1.6753453708447985 1102 78-79 1.9468566823906244 1102 80-81 2.1271194012444106 1102 82-83 2.0265390425523186 1102 84-85 2.2847389745699935 1102 86-87 2.4541373993648286 1102 88-89 2.712797346310616 1102 90-91 2.7621098535692745 1102 92-93 2.94579516615779 1102 94-95 3.105139101302452 1102 96-97 3.6732551666894047 1102 98-99 4.033756633872468 1102 100-101 4.470166952993498 1103 1 0.30089243956880196 1103 2 0.2977887551710623 1103 3 0.2844085597113235 1103 4 0.1923909354090796 1103 5 0.20120515703663244 1103 6 0.17512201674720984 1103 7 0.23009627789509324 1103 8 0.18937057116156097 1103 9 0.35701489577973433 1103 10-11 0.3696160467040457 1103 12-13 0.38721083750711927 1103 14-15 0.5913411793421588 1103 16-17 0.5429630454104881 1103 18-19 0.5199627148678445 1103 20-21 0.5447716885661222 1103 22-23 0.5116108869609164 1103 24-25 0.4427220270031711 1103 26-27 0.4882648041941948 1103 28-29 0.5125839473690519 1103 30-31 0.5562405113581903 1103 32-33 0.6418372675398274 1103 34-35 0.6062097807903299 1103 36-37 0.7570542716791451 1103 38-39 0.7326108274191512 1103 40-41 0.734954393207154 1103 42-43 0.7971184187872495 1103 44-45 0.9056108092553359 1103 46-47 0.9424397944816079 1103 48-49 0.9770011494871937 1103 50-51 1.066269979031695 1103 52-53 1.1936798555168764 1103 54-55 1.2354865266550803 1103 56-57 1.2428581336901061 1103 58-59 1.4649517610714184 1103 60-61 1.5746229819320234 1103 62-63 1.6714646380047427 1103 64-65 1.5051189989164797 1103 66-67 1.4865007789906741 1103 68-69 1.5629308800080395 1103 70-71 1.6487129033802432 1103 72-73 1.5825866127267076 1103 74-75 1.7022324396102242 1103 76-77 1.5627131065304134 1103 78-79 1.8919100518940937 1103 80-81 2.17879159139056 1103 82-83 2.3193304550178944 1103 84-85 2.4074982212230402 1103 86-87 2.513593646009511 1103 88-89 2.8149974399258433 1103 90-91 2.9509653994797596 1103 92-93 3.254809070666184 1103 94-95 3.3363492602515166 1103 96-97 3.728741985108666 1103 98-99 4.17794620041224 1103 100-101 4.492410410956349 1104 1 0.25056396849544527 1104 2 0.3048723249083096 1104 3 0.21525142419454824 1104 4 0.16417954886343722 1104 5 0.1061992226130073 1104 6 0.18835418479606147 1104 7 0.19556211482838393 1104 8 0.23961206064759466 1104 9 0.14517967997777248 1104 10-11 0.2763792575567692 1104 12-13 0.38732969800746986 1104 14-15 0.5221910324572434 1104 16-17 0.5351494861959765 1104 18-19 0.4583708808583822 1104 20-21 0.5474209713873464 1104 22-23 0.43437586066914236 1104 24-25 0.5012301003244488 1104 26-27 0.4014864247848067 1104 28-29 0.487907249542765 1104 30-31 0.5203484033621137 1104 32-33 0.5211670878492001 1104 34-35 0.5472894319479238 1104 36-37 0.6017310684214507 1104 38-39 0.7543466014407727 1104 40-41 0.779824205209728 1104 42-43 0.7898320174499034 1104 44-45 0.7594106197881558 1104 46-47 0.8541141319781218 1104 48-49 1.015111718029594 1104 50-51 0.9245229457865634 1104 52-53 1.1526749182097404 1104 54-55 1.2040872488065375 1104 56-57 1.2867714707844016 1104 58-59 1.2725777884137415 1104 60-61 1.3161519806148263 1104 62-63 1.4282986867051903 1104 64-65 1.535624108197723 1104 66-67 1.5105086647478316 1104 68-69 1.5590092898770962 1104 70-71 1.5330640556819972 1104 72-73 1.6974277358579073 1104 74-75 1.6281749291909797 1104 76-77 1.5232486249616422 1104 78-79 1.884156863707119 1104 80-81 2.089678525111129 1104 82-83 2.1732527278844067 1104 84-85 2.4200615772027696 1104 86-87 2.624203098789959 1104 88-89 2.8090448458585904 1104 90-91 2.977721054008679 1104 92-93 3.248661708804825 1104 94-95 3.4080805776763583 1104 96-97 3.8188384594084006 1104 98-99 4.2243761975985095 1104 100-101 4.5625746083243435 1105 1 0.2680785281750069 1105 2 0.2674010361433261 1105 3 0.24419037653458986 1105 4 0.1851095088092336 1105 5 0.17114966879616134 1105 6 0.2531184709117582 1105 7 0.16569986197718833 1105 8 0.22043541621568608 1105 9 0.33949647612602263 1105 10-11 0.31064925068056226 1105 12-13 0.40236638705410144 1105 14-15 0.5004643444081935 1105 16-17 0.4728663069395509 1105 18-19 0.43447196577501757 1105 20-21 0.4918491605009052 1105 22-23 0.43543616142860486 1105 24-25 0.3890223765103755 1105 26-27 0.5371170301985089 1105 28-29 0.6267409998698525 1105 30-31 0.6021321578962997 1105 32-33 0.635122733466595 1105 34-35 0.6920008884665592 1105 36-37 0.7765356286033906 1105 38-39 0.8089491882232664 1105 40-41 0.6828122592195811 1105 42-43 0.8868251111374192 1105 44-45 0.8367090673549669 1105 46-47 1.0155328894952902 1105 48-49 1.159602546101837 1105 50-51 1.0680722888377545 1105 52-53 1.1843990285788308 1105 54-55 1.1450988561744069 1105 56-57 1.2061877911662862 1105 58-59 1.427317231612264 1105 60-61 1.4464880683705985 1105 62-63 1.603697736528595 1105 64-65 1.6855432179498635 1105 66-67 1.758198230301403 1105 68-69 1.757626722616763 1105 70-71 1.7737666085714352 1105 72-73 1.8013527236244613 1105 74-75 1.876536140131396 1105 76-77 1.8164023932800966 1105 78-79 2.1587345387384183 1105 80-81 2.3315935327666004 1105 82-83 2.3807023359876673 1105 84-85 2.5477543872016604 1105 86-87 2.7519547772870006 1105 88-89 2.8471759652158575 1105 90-91 3.031053704481131 1105 92-93 3.2941200041724272 1105 94-95 3.517579888257412 1105 96-97 3.938000628148533 1105 98-99 4.156584858377162 1105 100-101 4.651484738729881 1106 1 0.20085050214249733 1106 2 0.18271303057226618 1106 3 0.16105011380980727 1106 4 0.13320089136045254 1106 5 0.11077613536110675 1106 6 0.12951933793173964 1106 7 0.21819755481595848 1106 8 0.06488827441292955 1106 9 0.12893579867309768 1106 10-11 0.22204852888064153 1106 12-13 0.12426524439187148 1106 14-15 0.11164304389647128 1106 16-17 0.08562256817297964 1106 18-19 0.20974310222700154 1106 20-21 0.31483677277692124 1106 22-23 0.25624369778002887 1106 24-25 0.14798455306279834 1106 26-27 0.1279758743267081 1106 28-29 0.36774347676553276 1106 30-31 0.3264612554922337 1106 32-33 0.2916648528133905 1106 34-35 0.23147568434655597 1106 36-37 0.3989060505276356 1106 38-39 0.40544816550603 1106 40-41 0.361269900003073 1106 42-43 0.5624520357368894 1106 44-45 0.17725945323078918 1106 46-47 -0.05905502904312954 1106 48-49 0.3530618314888798 1106 50-51 0.19698451062058098 1106 52-53 0.5077887902903271 1106 54-55 0.6133143441713358 1106 56-57 0.5666571507770328 1106 58-59 0.9608947316911269 1106 60-61 0.9581366589357145 1106 62-63 1.1149707002502467 1106 64-65 0.7602565989981684 1106 66-67 0.9555024294210526 1106 68-69 0.8224254719269446 1106 70-71 0.9541969622225643 1106 72-73 1.2931357913380097 1106 74-75 1.6132550409215867 1106 76-77 1.5397556826241292 1106 78-79 1.7448646024869632 1106 80-81 1.8581595346171973 1106 82-83 2.053638814937603 1106 84-85 2.1530001907748364 1106 86-87 2.518610534023207 1106 88-89 2.949316191326279 1106 90-91 2.7753167359738455 1106 92-93 2.3490313543102985 1106 94-95 2.8381488227054312 1106 96-97 3.3571960777439926 1106 98-99 3.7945256646173036 1106 100-101 4.002607648478321 1107 1 0.2242423897183521 1107 2 0.18100740690815798 1107 3 0.17664385382897052 1107 4 0.20601744050916437 1107 5 0.1243801448935784 1107 6 0.1606719311066911 1107 7 0.16150502600972771 1107 8 0.19764615466745283 1107 9 0.22084371700835703 1107 10-11 0.23837223966363297 1107 12-13 0.32357497586129114 1107 14-15 0.49693184324787154 1107 16-17 0.45842997794621 1107 18-19 0.4344161238715998 1107 20-21 0.4647005174640455 1107 22-23 0.39515773360047746 1107 24-25 0.43289642688046115 1107 26-27 0.4475634722924653 1107 28-29 0.4358187046357145 1107 30-31 0.3985626730781675 1107 32-33 0.49437964042118665 1107 34-35 0.4964683384506756 1107 36-37 0.538179715859421 1107 38-39 0.6657446055718736 1107 40-41 0.6464475036195054 1107 42-43 0.6958562565559916 1107 44-45 0.6580665190490294 1107 46-47 0.7061826684238852 1107 48-49 0.7229645413628418 1107 50-51 0.710028856246204 1107 52-53 0.8235209476251626 1107 54-55 0.8296842923324093 1107 56-57 0.8212461491309639 1107 58-59 0.9100480619124767 1107 60-61 0.8249642146441403 1107 62-63 0.9561274700215208 1107 64-65 1.0966938640646262 1107 66-67 1.24335713300556 1107 68-69 1.3006032475208826 1107 70-71 1.3783961612987987 1107 72-73 1.533668086254842 1107 74-75 1.620051444135111 1107 76-77 1.5802616404159373 1107 78-79 1.701555116699673 1107 80-81 2.0802603134295694 1107 82-83 2.1443198458198474 1107 84-85 2.412831554556373 1107 86-87 2.471214322441753 1107 88-89 2.637630566188083 1107 90-91 2.781676905985883 1107 92-93 2.9369034030536376 1107 94-95 3.2546339957884953 1107 96-97 3.5248194961652395 1107 98-99 3.9855564977254296 1107 100-101 4.391006980222908 1108 1 0.1699870060362194 1108 2 0.156987622022136 1108 3 0.1677606266527505 1108 4 0.13061266693878082 1108 5 0.09256595483734742 1108 6 0.018095976275958492 1108 7 0.0983187261100511 1108 8 0.05568427790303332 1108 9 0.17660919144898912 1108 10-11 0.1732433812608889 1108 12-13 0.21603086816431727 1108 14-15 0.37848686996213843 1108 16-17 0.3308533931721769 1108 18-19 0.33491443235716645 1108 20-21 0.2902587638885308 1108 22-23 0.3370240922948611 1108 24-25 0.3760145062533127 1108 26-27 0.26060474524455657 1108 28-29 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0.9270340912176209 2316 74-75 1.066896357286474 2316 76-77 1.0321809952546452 2316 78-79 1.1907945707939689 2316 80-81 1.376958824595821 2316 82-83 1.6254240641821305 2316 84-85 1.9173203883032741 2316 86-87 2.010278838570784 2316 88-89 1.9798514098605402 2316 90-91 2.2073518439511446 2316 92-93 2.203048564343959 2316 94-95 2.890872209188 2316 96-97 2.985455972592039 2316 98-99 3.2998170436311334 2316 100-101 3.9321843995777463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 12.0 4 36.0 5 102.0 6 227.0 7 542.0 8 1290.0 9 2324.0 10 3702.0 11 5990.0 12 8440.0 13 12077.0 14 15753.0 15 20508.0 16 25438.0 17 31242.0 18 30427.0 19 28299.0 20 28448.0 21 30349.0 22 33065.0 23 37090.0 24 43130.0 25 50356.0 26 59615.0 27 70763.0 28 84672.0 29 100468.0 30 118861.0 31 140739.0 32 168090.0 33 203725.0 34 254417.0 35 322633.0 36 408320.0 37 463462.0 38 265042.0 39 16206.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.83024030839945 19.410810831830787 15.090049217918589 23.668899641851173 2 15.768833869403723 20.401798291642393 39.547729716779116 24.28163812217477 3 19.18859677548978 23.57796714307565 30.309955331796868 26.9234807496377 4 13.229239478409486 17.0305998083522 36.79686337948889 32.94329733374942 5 13.630790071410637 37.395182328413675 34.766087505441604 14.20794009473409 6 32.73094224025712 36.56674991712221 16.942974572154554 13.759333270466115 7 28.74879004909152 32.563307126776934 20.357798727036048 18.3301040970955 8 26.387283452408383 35.619399268082994 19.45558933318297 18.537727946325656 9 26.006191448826893 16.64594316868279 19.891155457344432 37.45670992514588 10-11 24.862251119538776 26.55389778373612 27.659793724231324 20.924057372493778 12-13 25.77523543611216 23.614677097439976 27.863804328699633 22.746283137748232 14-15 22.244722637286287 25.4240539698866 25.911825809844814 26.419397582982295 16-17 22.123800459449185 28.544471589688353 26.94827615819077 22.38345179267169 18-19 22.18992445888014 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28.415955075187423 22.578377146886805 44-45 22.26618015518612 26.91277095877102 28.669232091674758 22.1518167943681 46-47 22.685580431162666 27.113916662926496 27.884365018501295 22.316137887409546 48-49 22.37139684153799 27.23960414392186 27.779630308350743 22.609368706189407 50-51 22.621601889127504 27.32460451172749 27.860920214046676 22.19287338509833 52-53 22.928079578232257 26.962793948803398 27.843502105889783 22.265624367074558 54-55 22.23754835956231 27.219565577729576 28.07686069578143 22.466025366926683 56-57 22.403332740307512 27.34625157451596 27.788315058092227 22.4621006270843 58-59 22.345358795204852 26.981978172112946 27.838300977779678 22.834362054902524 60-61 22.04354753879701 27.20353650786861 28.15304214145189 22.59987381188249 62-63 22.211765956364317 27.187965529320913 28.186306677308725 22.413961837006045 64-65 21.882604219427332 27.257297701230996 28.041517640573854 22.818580438767817 66-67 21.788570515651873 27.30738682008036 27.994763860070172 22.909278804197598 68-69 22.461602325961223 26.850016721410924 28.05898121239303 22.629399740234827 70-71 22.17336510396261 26.968610786783238 27.672772240169625 23.185251869084528 72-73 22.125638556153408 27.12803982542351 27.6780309462362 23.068290672186887 74-75 22.231420063521814 27.380131820242532 27.47065737398444 22.91779074225121 76-77 22.149644071086623 27.069101118680024 27.81326751070768 22.967987299525678 78-79 22.27936441890437 27.243363214705486 27.66609664895043 22.81117571743972 80-81 22.465474468532925 27.22617635845468 27.47066182502592 22.837687347986474 82-83 22.35344728412245 27.311100561112305 27.583117697478965 22.752334457286278 84-85 22.260790322708708 27.44402111714226 27.488645752501146 22.806542807647894 86-87 22.447930684103014 27.41357265642507 27.193808960324738 22.944687699147178 88-89 22.139492032711704 27.54191041224058 27.019869287992876 23.298728267054845 90-91 22.11842534004983 27.511666617247293 27.19180176383406 23.178106278868814 92-93 22.22808947965456 27.130266779460595 27.438381509745973 23.20326223113887 94-95 21.8832847408623 27.052298720392866 27.545644354961276 23.51877218378356 96-97 22.226542993237885 26.609765352968644 27.56755066401069 23.59614098978278 98-99 21.91172081510913 26.970960206023005 27.158330441117233 23.95898853775063 100-101 21.967739102565556 26.557897830769633 27.641297785595565 23.833065281069246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 297.0 1 200.0 2 157.5 3 229.5 4 223.0 5 221.0 6 400.0 7 897.0 8 1440.0 9 1661.5 10 1503.5 11 1369.5 12 1466.0 13 1605.0 14 1811.0 15 2057.0 16 2299.5 17 2539.0 18 2866.5 19 3366.5 20 4051.5 21 4857.0 22 5934.0 23 7549.0 24 9890.5 25 13005.0 26 16866.0 27 21677.5 28 26142.0 29 30696.0 30 36274.0 31 43845.5 32 55351.5 33 60586.5 34 61101.0 35 65693.0 36 71673.5 37 78239.5 38 83945.0 39 88276.0 40 93092.5 41 99347.0 42 104166.5 43 107994.5 44 111676.0 45 116081.5 46 117585.5 47 118384.0 48 119168.5 49 116696.5 50 113582.0 51 109984.0 52 105984.5 53 104987.0 54 105314.5 55 98102.5 56 87806.0 57 79195.5 58 72892.5 59 64034.0 60 50742.0 61 41652.0 62 36220.5 63 27582.5 64 18526.5 65 13400.5 66 9909.0 67 7551.0 68 5677.0 69 4241.5 70 3290.5 71 2308.5 72 1671.0 73 1378.5 74 1104.5 75 816.0 76 578.5 77 372.0 78 257.0 79 175.0 80 105.5 81 59.0 82 33.5 83 16.5 84 4.0 85 2.5 86 1.0 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.240578261747339E-5 2 0.04491441470781812 3 1.6202891308736696E-4 4 0.0 5 0.10159212850577909 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001069390826376622 26-27 0.011844313546686526 28-29 0.0 30-31 0.0 32-33 5.508983044970477E-4 34-35 0.015295529395447443 36-37 0.003321592718291023 38-39 0.012492429199035992 40-41 0.00735611265416646 42-43 0.005833040871145211 44-45 0.0029327233268813422 46-47 1.1342023916115687E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.6202891308736695E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.592462609397871E-4 68-69 1.6202891308736696E-4 70-71 0.0 72-73 3.240578261747339E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6202891308736695E-5 82-83 0.0011017966089940953 84-85 0.004196548848962804 86-87 0.0017661151526522996 88-89 3.240578261747339E-5 90-91 0.001117999500302832 92-93 1.7823180439610365E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.07361913289255E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3085869.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.75095202513842 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5840018455539 36.32956243431126 2 15.170007569607774 15.397846527723017 3 5.63459079436519 8.578825412583429 4 2.640368227527724 5.360048049758372 5 1.4351689917999784 3.6418096325403493 6 0.8817883925350143 2.6850960243521063 7 0.557548042415004 1.9807265766619595 8 0.37608959127267294 1.5269523843066675 9 0.27501204712372185 1.2561410888920004 >10 1.3204278176325266 11.947816776429656 >50 0.07933148726119492 2.7412753394695137 >100 0.03927274919804921 3.7113408718041896 >500 0.0030044485431316506 1.0166755562775405 >1k 0.0032601462900491026 3.3965424509606676 >5k 1.2784887411957264E-4 0.42934087392916065 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7263 0.23536319915070927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5971 0.19349492800893364 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4572 0.14815923812708834 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4445 0.14404370373466924 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4160 0.1348080556886893 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4075 0.1320535641662041 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3908 0.12664179846908602 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3829 0.12408174164230562 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3756 0.12171611951123007 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.240578261747339E-5 0.0 9 0.0 0.0 0.0 6.481156523494678E-5 0.0 10-11 0.0 0.0 0.0 6.481156523494678E-5 0.0 12-13 0.0 0.0 0.0 9.721734785242018E-5 3.240578261747339E-5 14-15 0.0 0.0 0.0 9.721734785242018E-5 3.240578261747339E-5 16-17 0.0 0.0 0.0 1.2962313046989356E-4 3.240578261747339E-5 18-19 1.6202891308736695E-5 0.0 0.0 1.4582602177863027E-4 3.240578261747339E-5 20-21 3.240578261747339E-5 0.0 0.0 1.9443469570484036E-4 6.481156523494678E-5 22-23 3.240578261747339E-5 0.0 0.0 3.72666500100944E-4 6.481156523494678E-5 24-25 3.240578261747339E-5 0.0 0.0 5.671011958057843E-4 6.481156523494678E-5 26-27 3.240578261747339E-5 0.0 0.0 0.0010369850437591485 6.481156523494678E-5 28-29 6.481156523494678E-5 0.0 0.0 0.0032243753704386023 6.481156523494678E-5 30-31 6.481156523494678E-5 0.0 0.0 0.012427617633801047 8.101445654368348E-5 32-33 6.481156523494678E-5 0.0 0.0 0.03161184094334529 9.721734785242018E-5 34-35 6.481156523494678E-5 0.0 0.0 0.061570986973199446 9.721734785242018E-5 36-37 6.481156523494678E-5 0.0 0.0 0.1117999500302832 9.721734785242018E-5 38-39 6.481156523494678E-5 0.0 0.0 0.1868679454636603 1.2962313046989356E-4 40-41 6.481156523494678E-5 0.0 0.0 0.2675745470724778 1.6202891308736696E-4 42-43 6.481156523494678E-5 0.0 0.0 0.35213743681277465 1.7823180439610365E-4 44-45 6.481156523494678E-5 0.0 0.0 0.45562530360167586 2.4304336963105046E-4 46-47 6.481156523494678E-5 0.0 0.0 0.5793181758525718 2.9165204355726055E-4 48-49 6.481156523494678E-5 0.0 0.0 0.7055549020389394 2.9165204355726055E-4 50-51 6.481156523494678E-5 0.0 0.0 0.8480269253166612 2.9165204355726055E-4 52-53 8.101445654368348E-5 0.0 0.0 0.9840664007448146 3.564636087922073E-4 54-55 9.721734785242018E-5 0.0 0.0 1.1328575516329435 3.564636087922073E-4 56-57 9.721734785242018E-5 0.0 0.0 1.3077029517455214 3.564636087922073E-4 58-59 9.721734785242018E-5 0.0 0.0 1.495040781057135 3.564636087922073E-4 60-61 9.721734785242018E-5 0.0 0.0 1.7015304278956753 3.564636087922073E-4 62-63 9.721734785242018E-5 0.0 0.0 1.902041207841292 3.564636087922073E-4 64-65 9.721734785242018E-5 0.0 0.0 2.1172156044213155 3.72666500100944E-4 66-67 9.721734785242018E-5 0.0 0.0 2.3678419271848545 3.8886939140968073E-4 68-69 9.721734785242018E-5 0.0 0.0 2.6419948481286797 3.8886939140968073E-4 70-71 9.721734785242018E-5 0.0 0.0 2.9332418194032215 3.8886939140968073E-4 72-73 9.721734785242018E-5 0.0 0.0 3.2314560339405203 4.212751740271541E-4 74-75 9.721734785242018E-5 0.0 0.0 3.5333645076962115 4.536809566446275E-4 76-77 9.721734785242018E-5 0.0 0.0 3.8707735163093444 4.536809566446275E-4 78-79 9.721734785242018E-5 0.0 0.0 4.246097290584921 4.536809566446275E-4 80-81 1.1342023916115687E-4 0.0 0.0 4.640589085278734 4.536809566446275E-4 82-83 1.6202891308736696E-4 0.0 0.0 5.049614873476482 4.536809566446275E-4 84-85 1.6202891308736696E-4 0.0 0.0 5.470484975220918 4.860867392621009E-4 86-87 1.6202891308736696E-4 0.0 0.0 5.9012550435549915 5.184925218795742E-4 88-89 1.6202891308736696E-4 0.0 0.0 6.363345300788854 5.508983044970477E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4460 0.0 57.054462 1 GTATCAA 7770 0.0 39.727783 1 ATCAACG 9780 0.0 31.109526 3 TCAACGC 10075 0.0 30.151438 4 TATCAAC 10170 0.0 30.071886 2 CAACGCA 10265 0.0 29.639664 5 AACGCAG 10685 0.0 28.447384 6 ACGCAGA 12145 0.0 24.949396 7 CGCAGAG 12315 0.0 24.450722 8 TGGTATC 1865 0.0 23.207758 2 TAAGGTG 2105 0.0 22.809801 5 GTACATG 8715 0.0 21.557596 1 TACATGG 8420 0.0 21.35258 2 GTAAGGT 2135 0.0 21.153292 4 GCAGAGT 14070 0.0 20.89457 9 GTGTAAG 1475 0.0 20.637571 1 GAGTACT 9350 0.0 20.521383 12-13 CAGAGTA 13280 0.0 20.152676 10-11 ACATGGG 8525 0.0 20.075222 3 AGAGTAC 12755 0.0 19.80925 10-11 >>END_MODULE