FastQCFastQC Report
Thu 26 May 2016
SRR938416_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938416_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080416
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68790.22331399395406334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54220.17601518755908294No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44400.14413637638552715No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA42900.1392669042103404No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41330.13417019000031166No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA37790.12267823566687096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36120.11725688997849641No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA34030.11047209208106958No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33070.10735562988895006No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32340.10498582009702585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.052.0315131
GTATCAA79700.041.914651
ATCAACG97900.033.1559223
TCAACGC99700.032.557324
TATCAAC102100.031.9782072
CAACGCA102750.031.6371545
AACGCAG108450.030.0118276
TGGTATC20750.026.339222
ACGCAGA122850.026.2941637
CGCAGAG124600.025.810498
GTACATG90200.024.4433961
TACATGG88900.023.8962022
GTGGTAT24700.023.7648071
ACATGGG87900.023.1948493
GCAGAGT138250.022.6085269
TAAGGTG21200.022.193335
GTAAGGT21350.021.1470054
GTGTAGC18700.020.9265841
CAGAGTA130200.020.5027510-11
AGAGTAC125650.020.47023410-11
Produced by FastQC (version 0.11.5)