##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938416_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3080416 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.075405399790156 31.0 31.0 34.0 28.0 34.0 2 31.25338298463584 31.0 31.0 34.0 28.0 34.0 3 31.312744122871717 31.0 31.0 34.0 27.0 34.0 4 34.97439241972513 37.0 35.0 37.0 32.0 37.0 5 34.97311239780601 37.0 35.0 37.0 32.0 37.0 6 34.94314793846026 37.0 35.0 37.0 32.0 37.0 7 34.860369183902435 37.0 35.0 37.0 32.0 37.0 8 34.81013863062651 37.0 35.0 37.0 32.0 37.0 9 36.300866506342004 39.0 35.0 39.0 32.0 39.0 10-11 36.135248615771374 39.0 35.0 39.0 31.0 39.0 12-13 35.95083569881471 38.0 35.0 39.0 30.0 39.0 14-15 37.121640875777814 39.0 36.0 41.0 31.0 41.0 16-17 37.04764129260464 39.0 36.0 41.0 31.0 41.0 18-19 37.03782654680407 39.0 36.0 41.0 31.0 41.0 20-21 36.966655477701714 39.0 36.0 41.0 30.5 41.0 22-23 36.81737271848997 39.0 36.0 41.0 30.0 41.0 24-25 36.67447448656285 39.0 36.0 41.0 30.0 41.0 26-27 36.45885458327706 39.0 36.0 41.0 30.0 41.0 28-29 36.229286076945456 39.0 35.0 40.5 29.0 41.0 30-31 35.950148778606525 38.5 35.0 40.0 28.0 41.0 32-33 35.647716736960206 38.0 34.5 40.0 27.0 41.0 34-35 35.343662674132325 38.0 34.0 40.0 26.5 41.0 36-37 35.10680554184889 38.0 34.0 40.0 25.0 41.0 38-39 34.86165764623999 38.0 34.0 40.0 25.0 41.0 40-41 34.57635949170502 38.0 33.0 40.0 24.0 41.0 42-43 34.154529615480506 38.0 33.0 40.0 23.5 41.0 44-45 33.69778416291825 38.0 32.5 40.0 21.5 41.0 46-47 33.22723619147544 37.5 31.5 40.0 18.5 41.0 48-49 32.745353711966175 37.0 31.0 40.0 15.5 41.0 50-51 32.021038716848636 36.5 30.5 39.0 13.5 40.5 52-53 32.007019506456274 36.5 30.5 39.0 11.0 40.0 54-55 31.975945619033276 36.5 30.5 39.0 8.5 40.0 56-57 31.5484723491892 36.0 30.0 39.0 7.5 40.0 58-59 31.086107688052522 36.0 29.0 39.0 2.0 40.0 60-61 30.529538055898946 35.0 28.5 39.0 2.0 40.0 62-63 29.792672320881337 34.5 27.5 38.0 2.0 40.0 64-65 29.131603166585293 34.0 26.5 38.0 2.0 40.0 66-67 28.4250427539657 34.0 26.0 37.5 2.0 40.0 68-69 27.574335089806052 33.0 24.0 36.5 2.0 39.0 70-71 26.71752711322107 32.5 21.5 36.0 2.0 39.0 72-73 25.802420192597364 31.5 19.0 35.5 2.0 38.5 74-75 24.850233215254043 31.0 12.0 35.0 2.0 37.5 76-77 23.881535480922057 30.5 7.0 34.5 2.0 37.0 78-79 22.880195402179446 29.5 2.0 34.0 2.0 36.0 80-81 21.89812544799144 29.0 2.0 34.0 2.0 36.0 82-83 20.868036979420964 27.5 2.0 34.0 2.0 35.0 84-85 19.854162554667944 26.0 2.0 33.0 2.0 35.0 86-87 18.83915776310732 25.5 2.0 32.5 2.0 35.0 88-89 17.717499519545413 22.0 2.0 32.0 2.0 34.0 90-91 16.551817514257813 17.5 2.0 31.0 2.0 34.0 92-93 15.385812662965002 4.5 2.0 31.0 2.0 34.0 94-95 14.121706126704964 2.0 2.0 30.5 2.0 34.0 96-97 12.707713503630679 2.0 2.0 29.5 2.0 34.0 98-99 11.013181174231013 2.0 2.0 27.5 2.0 33.5 100-101 7.737722599804702 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5705831552913416 1101 2 0.48281034008552126 1101 3 0.46791862203970425 1101 4 0.4547336626580005 1101 5 0.40604116337935636 1101 6 0.5604193138684295 1101 7 0.5609281676074431 1101 8 0.4587251066582141 1101 9 0.587085026142276 1101 10-11 0.5608110197691616 1101 12-13 0.5733316598155369 1101 14-15 0.704448799699982 1101 16-17 0.6794519105773844 1101 18-19 0.6553494575752907 1101 20-21 0.866087148563139 1101 22-23 0.8664936764599247 1101 24-25 0.853539125332901 1101 26-27 0.9098766629453152 1101 28-29 0.9076570972604472 1101 30-31 1.0147747805313685 1101 32-33 0.9536017626017781 1101 34-35 1.0530501318941887 1101 36-37 1.1988941939008626 1101 38-39 1.197544463723581 1101 40-41 1.261773543346905 1101 42-43 1.4042323224351563 1101 44-45 1.487472583597338 1101 46-47 1.5557870256667172 1101 48-49 1.440459792536906 1101 50-51 1.5876106767743465 1101 52-53 1.6182882719620792 1101 54-55 1.750034235878882 1101 56-57 1.9836315027282296 1101 58-59 1.9073696602000574 1101 60-61 2.0223315008092726 1101 62-63 1.8587586655770671 1101 64-65 2.049905623383257 1101 66-67 2.387685459157968 1101 68-69 2.4733202691076457 1101 70-71 2.588653498930981 1101 72-73 2.743994928958532 1101 74-75 2.8998693506328053 1101 76-77 3.17967087286738 1101 78-79 3.2095472514209114 1101 80-81 3.5345747970159387 1101 82-83 3.717158853716441 1101 84-85 3.7740085031868276 1101 86-87 3.7932954565940307 1101 88-89 3.90517634618093 1101 90-91 3.76828709479161 1101 92-93 4.037549740678749 1101 94-95 3.810082381922337 1101 96-97 3.7353246882616755 1101 98-99 3.4178098189679655 1101 100-101 2.3103227786096916 1102 1 0.609926375630323 1102 2 0.5131137845907148 1102 3 0.5225919407931201 1102 4 0.3240814392207838 1102 5 0.325456144243212 1102 6 0.4496755103327956 1102 7 0.48547095054894385 1102 8 0.47890211595291277 1102 9 0.47395763412477976 1102 10-11 0.5821623957487461 1102 12-13 0.6170294910530103 1102 14-15 0.6803213165580075 1102 16-17 0.8058526076796255 1102 18-19 0.7949644566640544 1102 20-21 0.7505364150111049 1102 22-23 0.6739652000611613 1102 24-25 0.6429833799346909 1102 26-27 0.6984463503883589 1102 28-29 0.658316268938961 1102 30-31 0.6939309678832899 1102 32-33 0.7739470104603612 1102 34-35 0.8208931016408698 1102 36-37 0.8156563134561736 1102 38-39 1.0031616042375262 1102 40-41 1.0352177068242199 1102 42-43 1.2235323725536134 1102 44-45 1.1200984492798582 1102 46-47 1.1113041570682114 1102 48-49 1.197177147193365 1102 50-51 1.387229890370115 1102 52-53 1.3221766672600523 1102 54-55 1.3701563426767613 1102 56-57 1.7096497048423203 1102 58-59 1.7039006653030206 1102 60-61 1.7215278968362426 1102 62-63 1.7906220472063694 1102 64-65 1.827584341261879 1102 66-67 2.0296773262984793 1102 68-69 2.1425395601592214 1102 70-71 2.0214735736530507 1102 72-73 2.0767113495891145 1102 74-75 2.147523123186115 1102 76-77 2.188635584914035 1102 78-79 2.7256684241929214 1102 80-81 3.050195924225708 1102 82-83 3.1316458229163686 1102 84-85 3.1982639023120356 1102 86-87 3.3612064279809424 1102 88-89 3.4200297496801113 1102 90-91 3.479282743597878 1102 92-93 3.4660714738662826 1102 94-95 3.3153223810110948 1102 96-97 2.97453851184469 1102 98-99 2.5761857529938457 1102 100-101 1.9218483162441 1103 1 0.4947458641023488 1103 2 0.394228775680336 1103 3 0.40074579890434237 1103 4 0.2744904284339498 1103 5 0.28831190712162424 1103 6 0.368016569696465 1103 7 0.357271069354006 1103 8 0.3394073150624308 1103 9 0.40433775481739787 1103 10-11 0.605275921205795 1103 12-13 0.5238095962010476 1103 14-15 0.580756565021602 1103 16-17 0.5153835497371659 1103 18-19 0.7285933407291836 1103 20-21 0.7470157323816338 1103 22-23 0.6976665885259692 1103 24-25 0.7398645249046325 1103 26-27 0.8748404501068947 1103 28-29 0.7747773214185969 1103 30-31 0.7384724302959214 1103 32-33 0.9603363357697248 1103 34-35 0.899266582573766 1103 36-37 0.9584684404554551 1103 38-39 1.0396237115284919 1103 40-41 1.1280924007901447 1103 42-43 1.2413659566359527 1103 44-45 1.200529534014521 1103 46-47 1.4108385966580599 1103 48-49 1.3719225768069165 1103 50-51 1.4593760983233963 1103 52-53 1.5073286988821835 1103 54-55 1.5139267935253322 1103 56-57 1.5710167403228397 1103 58-59 1.6522798644236438 1103 60-61 1.9195390223199311 1103 62-63 1.9695227672538955 1103 64-65 2.0164048956485026 1103 66-67 2.1319287933189734 1103 68-69 2.2974130660744905 1103 70-71 2.3404854930210526 1103 72-73 2.4685277346951757 1103 74-75 2.3278146952436067 1103 76-77 2.5289070045235356 1103 78-79 2.696251068660434 1103 80-81 2.917699352362522 1103 82-83 3.1175441613109456 1103 84-85 3.1672142540083357 1103 86-87 3.232901176686024 1103 88-89 3.4659872685418023 1103 90-91 3.445324036685534 1103 92-93 3.5937276275800514 1103 94-95 3.4866518874149097 1103 96-97 3.3902237783511016 1103 98-99 3.2156493536755875 1103 100-101 2.367164398785862 1104 1 0.4976664886246738 1104 2 0.37317720862257886 1104 3 0.4098989087780538 1104 4 0.35688703265758903 1104 5 0.34165892797376074 1104 6 0.4282881908098801 1104 7 0.43180707627139014 1104 8 0.400892072010663 1104 9 0.5511191381185441 1104 10-11 0.5319414939585911 1104 12-13 0.6038388076753023 1104 14-15 0.6768486884652773 1104 16-17 0.6665871791885962 1104 18-19 0.6465099033380781 1104 20-21 0.6148891868361162 1104 22-23 0.7256105449969823 1104 24-25 0.6799556941542306 1104 26-27 0.7781131036407416 1104 28-29 0.8569970274297702 1104 30-31 0.809773338600003 1104 32-33 0.8248945828135419 1104 34-35 0.6739567204905725 1104 36-37 0.8271861747025682 1104 38-39 0.9059022583925511 1104 40-41 0.8341037816775625 1104 42-43 0.8232582205524039 1104 44-45 0.7515281958001978 1104 46-47 0.7559296738766648 1104 48-49 0.8054351509598447 1104 50-51 0.9151138438735913 1104 52-53 0.91495985664465 1104 54-55 1.1845293539112554 1104 56-57 1.3549526465917836 1104 58-59 1.4429383733793024 1104 60-61 1.6256915871191637 1104 62-63 1.6279215471739015 1104 64-65 1.670942243290149 1104 66-67 1.9728527489449696 1104 68-69 2.272432193880025 1104 70-71 2.2207048007890116 1104 72-73 2.4218108149223596 1104 74-75 2.475868145590166 1104 76-77 2.4993785985296384 1104 78-79 2.609022105167874 1104 80-81 2.7034783884549896 1104 82-83 2.747995585887992 1104 84-85 2.887731183737234 1104 86-87 2.9179684471596765 1104 88-89 3.0246191169550407 1104 90-91 3.006913217438175 1104 92-93 3.3527366252830397 1104 94-95 3.312238198055491 1104 96-97 3.1719196188429795 1104 98-99 2.809012235232954 1104 100-101 2.089057767074241 1105 1 0.46300295184846263 1105 2 0.41415089235973923 1105 3 0.43384257074988497 1105 4 0.3456554027761314 1105 5 0.2985168057641303 1105 6 0.3988933029642894 1105 7 0.41349991529789065 1105 8 0.39507569225062156 1105 9 0.46625863409825286 1105 10-11 0.5552668226870239 1105 12-13 0.396131902129639 1105 14-15 0.5539559681587676 1105 16-17 0.6405609708530164 1105 18-19 0.68393053183447 1105 20-21 0.5323270836936373 1105 22-23 0.5650975307685755 1105 24-25 0.7072954671472331 1105 26-27 0.517581651839258 1105 28-29 0.5460842326534561 1105 30-31 0.48022735259456795 1105 32-33 0.7138756595678117 1105 34-35 0.7406575641219675 1105 36-37 0.7741178927246253 1105 38-39 0.8462328755565878 1105 40-41 0.9266693924558993 1105 42-43 0.8920883790255942 1105 44-45 1.098524220479561 1105 46-47 1.102111297462372 1105 48-49 1.0791831947783166 1105 50-51 1.061963717059065 1105 52-53 1.1511690016808913 1105 54-55 1.386474742719198 1105 56-57 1.4521573306347406 1105 58-59 1.3622227257258253 1105 60-61 1.5438575432647212 1105 62-63 1.4397706109049473 1105 64-65 1.5944483501741082 1105 66-67 1.7529936839547737 1105 68-69 1.860305667159036 1105 70-71 2.037836004357807 1105 72-73 2.220509392081354 1105 74-75 2.367189445034338 1105 76-77 2.3433081400186744 1105 78-79 2.432448615699773 1105 80-81 2.6987853515618525 1105 82-83 2.805628741141348 1105 84-85 3.002108893161079 1105 86-87 3.1427130193180446 1105 88-89 3.0625371102275736 1105 90-91 3.0409458294576694 1105 92-93 3.1415166875210936 1105 94-95 3.128545107198727 1105 96-97 3.0766742642120004 1105 98-99 2.9248788911068946 1105 100-101 2.052599489953021 1106 1 0.2934919751281271 1106 2 0.2748212547727604 1106 3 0.187362813360604 1106 4 0.29858238084037225 1106 5 0.2588396972877831 1106 6 -0.11455550033889494 1106 7 0.3562556335370175 1106 8 0.016240471257368938 1106 9 0.33536378603940165 1106 10-11 0.20509397139219487 1106 12-13 0.3514739354974523 1106 14-15 0.45499174970302647 1106 16-17 0.020460266185580167 1106 18-19 0.4353787692817477 1106 20-21 0.4535935399326334 1106 22-23 0.3273487531057313 1106 24-25 0.3662664935567861 1106 26-27 0.3241902298610526 1106 28-29 0.4211976248711693 1106 30-31 0.32235532444323667 1106 32-33 0.47592441159029875 1106 34-35 0.3790349723251083 1106 36-37 0.6297680523136719 1106 38-39 0.7203869651917003 1106 40-41 0.7751172674017468 1106 42-43 0.37264725014306777 1106 44-45 0.8010714561850705 1106 46-47 0.8673681872000429 1106 48-49 0.7675349626359278 1106 50-51 0.7953089864153142 1106 52-53 0.8690504902355727 1106 54-55 0.7769108938380889 1106 56-57 1.2070696671775352 1106 58-59 1.214603741260337 1106 60-61 1.5139635528312212 1106 62-63 1.5077595236658787 1106 64-65 1.5366992188513322 1106 66-67 1.6695078347730572 1106 68-69 1.8786349776105702 1106 70-71 1.8560247349838725 1106 72-73 1.9319467544855335 1106 74-75 2.2322186661867427 1106 76-77 2.465239476941658 1106 78-79 2.772598744343579 1106 80-81 2.707044637345607 1106 82-83 2.901852979225218 1106 84-85 2.9968075306861692 1106 86-87 2.7281806903789345 1106 88-89 2.7839406096926638 1106 90-91 2.806231896140254 1106 92-93 2.835895704876954 1106 94-95 2.8323531405842264 1106 96-97 2.6930005331880675 1106 98-99 2.4331788477835516 1106 100-101 1.6354704129170443 1107 1 0.19678982905236353 1107 2 0.05152791283535052 1107 3 0.02911785408395673 1107 4 0.03534888953024051 1107 5 0.10847792717656546 1107 6 0.06538096920701264 1107 7 0.06313156554190869 1107 8 0.07485297423243509 1107 9 0.10329497327788317 1107 10-11 0.13239821004498964 1107 12-13 0.09113701948625419 1107 14-15 0.20057939304305705 1107 16-17 0.27421319329886273 1107 18-19 0.24623505047701855 1107 20-21 0.2659488065988853 1107 22-23 0.25546119853579086 1107 24-25 0.1448557227723981 1107 26-27 0.12491372236789289 1107 28-29 0.26050404431759944 1107 30-31 0.3133122645527209 1107 32-33 0.45593138570191627 1107 34-35 0.4393224836074623 1107 36-37 0.5121492972593984 1107 38-39 0.6698988537234882 1107 40-41 0.5888408265195508 1107 42-43 0.8431814854156983 1107 44-45 0.6867254184786802 1107 46-47 0.7422858062222986 1107 48-49 0.5704388670505196 1107 50-51 0.7132871639734475 1107 52-53 0.9284731563273638 1107 54-55 1.0805093941941486 1107 56-57 1.243712811963647 1107 58-59 1.365956516599848 1107 60-61 1.4739535478249053 1107 62-63 1.5467740381778157 1107 64-65 1.492591684692215 1107 66-67 1.6542360763178046 1107 68-69 1.7647739212292493 1107 70-71 1.8826238898070535 1107 72-73 1.9512918980016458 1107 74-75 2.053655737808043 1107 76-77 2.0533691343417644 1107 78-79 2.4537647493857797 1107 80-81 2.6116759990514 1107 82-83 2.5499576584647556 1107 84-85 2.3345866215971682 1107 86-87 2.2400340557695166 1107 88-89 2.6121028129469828 1107 90-91 2.4695484984767546 1107 92-93 2.545729192713388 1107 94-95 2.3829201259338237 1107 96-97 2.168551013712756 1107 98-99 1.98106941759748 1107 100-101 1.3855606908260398 1108 1 0.32129230809013265 1108 2 0.2739076511001137 1108 3 0.2093105746042454 1108 4 0.16865796682405687 1108 5 0.1031642906059389 1108 6 0.14971756269311243 1108 7 0.20199650248617473 1108 8 0.1999703484716946 1108 9 0.15546382659125157 1108 10-11 0.12981977157890157 1108 12-13 0.20419480298409098 1108 14-15 0.32921231376306537 1108 16-17 0.310992739477868 1108 18-19 0.3190776390533401 1108 20-21 0.30134566653037353 1108 22-23 0.30437931324355105 1108 24-25 0.3720295093226227 1108 26-27 0.34731632141326685 1108 28-29 0.5674411182940702 1108 30-31 0.5348022722757122 1108 32-33 0.5546016426304163 1108 34-35 0.6666368386597625 1108 36-37 0.7007390078842306 1108 38-39 0.7871963230764649 1108 40-41 0.8115729979855288 1108 42-43 0.7945539372091233 1108 44-45 0.7139821386052247 1108 46-47 0.6930365091798052 1108 48-49 0.9031616596167638 1108 50-51 0.8516869277300501 1108 52-53 1.0389401740166377 1108 54-55 1.0804484014475015 1108 56-57 1.2718723259351634 1108 58-59 1.3054417304585364 1108 60-61 1.411705850117098 1108 62-63 1.4650893412395511 1108 64-65 1.6800513275948603 1108 66-67 1.5575306045876687 1108 68-69 1.8930947025987734 1108 70-71 2.136477035280272 1108 72-73 2.1910981287372984 1108 74-75 2.143668152545839 1108 76-77 2.219974593606569 1108 78-79 2.601216002335949 1108 80-81 2.5402528487622327 1108 82-83 2.831288634270507 1108 84-85 2.987431316861123 1108 86-87 3.052378509316366 1108 88-89 3.170334372875576 1108 90-91 2.9994714663856428 1108 92-93 3.0475489476476625 1108 94-95 2.99949607777255 1108 96-97 2.816169835724791 1108 98-99 2.598948387933204 1108 100-101 1.907703081750908 1109 1 0.6677814311534114 1109 2 0.5044288050465902 1109 3 0.5352628934523835 1109 4 0.46433275424124076 1109 5 0.39915573512573843 1109 6 0.5603826991112868 1109 7 0.4927649306775237 1109 8 0.4907899008740131 1109 9 0.5533030461645225 1109 10-11 0.6306515833583362 1109 12-13 0.541144043946062 1109 14-15 0.6642050103043573 1109 16-17 0.7212295802103057 1109 18-19 0.7473081153431806 1109 20-21 0.6676988930940979 1109 22-23 0.9148506471681159 1109 24-25 0.7168203173981595 1109 26-27 0.6926661049178833 1109 28-29 0.7202537324106117 1109 30-31 0.764109226211751 1109 32-33 0.9200925393908079 1109 34-35 0.9841163628915908 1109 36-37 0.9563751913053835 1109 38-39 1.03307978074249 1109 40-41 0.8287653397873953 1109 42-43 1.0017001107184313 1109 44-45 1.1512089109981503 1109 46-47 1.298452023812814 1109 48-49 1.2406458789291932 1109 50-51 1.3949797442567373 1109 52-53 1.450394083975425 1109 54-55 1.6519623041028275 1109 56-57 1.59524000291362 1109 58-59 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0.15731018853176248 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10489.0 3 3878.0 4 4094.0 5 4809.0 6 5847.0 7 7333.0 8 9665.0 9 12303.0 10 16074.0 11 20244.0 12 24813.0 13 29328.0 14 33954.0 15 38871.0 16 44204.0 17 50380.0 18 51229.0 19 51971.0 20 56276.0 21 62142.0 22 69443.0 23 77237.0 24 85379.0 25 94034.0 26 104958.0 27 114979.0 28 127372.0 29 140068.0 30 156046.0 31 173942.0 32 192680.0 33 217334.0 34 242179.0 35 262933.0 36 257092.0 37 180517.0 38 45697.0 39 622.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14093558160748 18.600274337157042 14.492191102650914 23.76659897858456 2 15.363313259760902 19.662575641215067 40.64058996494567 24.333521134078367 3 19.20238624886744 23.392383358609557 29.82005709071188 27.58517330181112 4 12.698250826390185 16.346319356529634 37.15612002907386 33.79930978800631 5 13.701204371547778 37.344516009994635 34.59852549268268 14.355754125774903 6 32.76517735223462 36.10960616253705 17.247555162320072 13.877661322908263 7 28.911494814291355 32.50365675597032 20.39567008916509 18.189178340573235 8 26.36290033700454 35.23501495687011 19.675323388933506 18.726761317191844 9 26.21478907543922 16.82388854609814 19.986133212093655 36.975189166368985 10-11 24.907887397813912 26.385180145473853 27.724192355437488 20.982740101274743 12-13 25.929832257976525 23.535612118834734 27.817268954696033 22.717286668492708 14-15 22.43605667427489 25.311675715028397 25.939881729972726 26.312385880723987 16-17 22.240954004778985 28.42712339024195 26.915791076693985 22.41613152828508 18-19 22.27481049388489 26.955410211249315 28.945110768207655 21.824668526658144 20-21 22.59348713656295 26.336609709623808 29.207702555800825 21.862200598012414 22-23 22.297035091724425 26.23470190658076 29.05112283711011 22.417140164584705 24-25 22.179428846459253 26.417661831870426 29.229310030700162 22.173599290970163 26-27 21.832972943394505 26.815739338457544 28.864416904666918 22.486870813481037 28-29 21.971396163012386 27.268402411212143 28.663734105506606 22.096467320268868 30-31 22.144049412865012 26.744355970071705 28.608103631923516 22.503490985139766 32-33 21.7420607228828 26.744186046511626 28.66277364962392 22.85097958098165 34-35 22.54960460013103 26.965402409986762 28.405662973670047 22.079330016212165 36-37 22.726830640904343 26.420854148356188 28.443451684602213 22.40886352613726 38-39 22.12678044894611 26.75520500112583 28.43395169364143 22.684062856286626 40-41 22.74719880156661 26.566880550489753 28.478085052017505 22.20783559592613 42-43 22.36025439622779 26.610223998485715 28.33882873644257 22.69069286884393 44-45 22.336329835105822 26.886682493321505 28.5196828868234 22.25730478474927 46-47 22.740498322603102 27.07435810183351 27.768159461154916 22.41698411440847 48-49 22.40763180520597 27.200595613809515 27.683216834077527 22.708555746906992 50-51 22.65785901886998 27.309487295744017 27.71456182293115 22.318091862454853 52-53 22.925671719371405 26.92182509039075 27.821942115831 22.330561074406848 54-55 22.239883745619522 27.19920055633799 28.045200513482822 22.515715184559674 56-57 22.45077749393246 27.28698438908478 27.76448194153443 22.49775617544833 58-59 22.418617999355906 26.859955952170704 27.830855426505575 22.890570621967814 60-61 22.052214147839713 27.111722835723427 28.12718280633229 22.70888021010457 62-63 22.250997357965268 27.03906234146777 28.27045344783503 22.43948685273193 64-65 21.920037686311133 27.10674287257803 28.121429268283972 22.851790172826863 66-67 21.912253915775004 27.125176565766225 28.00911730264971 22.953452215809058 68-69 22.520756238559052 26.698089762138626 28.129984934510325 22.651169064791997 70-71 22.319160924938405 26.782181424381506 27.713980195846734 23.184677454833352 72-73 22.30576253951598 26.92513480967883 27.76892700618782 23.000175644617368 74-75 22.353830195141985 27.205270018652207 27.569125976237512 22.871773809968296 76-77 22.458756168536404 26.795143313924186 27.722552255118377 23.023548262421034 78-79 22.532493927172826 27.00388686912238 27.527357753641585 22.93626145006321 80-81 22.798429947044234 27.042813147367216 27.235279211343677 22.923477694244866 82-83 22.66969049337131 27.114975490932174 27.354554774256023 22.860779241440497 84-85 22.676286161576087 27.15618865932456 27.303633543215977 22.863891635883373 86-87 22.84848286129639 27.116034019643298 26.98285058328015 23.052632535780166 88-89 22.645995580407554 27.099257708474433 26.887482864825795 23.36726384629221 90-91 22.67614789838253 27.15209434386231 26.89483924681812 23.276918510937037 92-93 22.913331055255824 26.682093742618907 27.155344053284285 23.24923114884098 94-95 22.61305692362042 26.573101420363038 27.14963685670646 23.664204799310077 96-97 22.960552184349776 26.131073000197087 27.13099474940427 23.777380066048863 98-99 22.728254111520375 26.360044349713398 26.65742443747129 24.254277101294942 100-101 23.683270941692594 25.314272666773384 27.04970556616859 23.95275082536543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 191.0 2 169.0 3 207.5 4 196.5 5 206.5 6 331.0 7 691.0 8 1178.5 9 1358.5 10 1216.5 11 1149.5 12 1246.5 13 1413.0 14 1603.5 15 1809.0 16 2049.5 17 2362.0 18 2787.0 19 3276.0 20 3912.0 21 4742.0 22 5887.0 23 7485.5 24 9740.5 25 12682.0 26 16828.0 27 21675.0 28 26027.0 29 30622.0 30 35917.0 31 43404.0 32 53365.0 33 58623.5 34 60611.0 35 65476.0 36 71313.0 37 77294.0 38 82780.5 39 86959.0 40 91738.5 41 97866.0 42 102715.0 43 106136.0 44 109122.0 45 113201.0 46 115598.5 47 116997.0 48 117771.0 49 115399.5 50 112284.0 51 109278.5 52 106053.5 53 104236.5 54 103007.5 55 96783.0 56 88304.0 57 80830.5 58 74739.0 59 65926.0 60 53773.0 61 44759.0 62 38169.5 63 30116.0 64 21777.5 65 16155.5 66 12105.0 67 9034.5 68 6781.5 69 5186.0 70 4103.5 71 3040.5 72 2166.0 73 1677.5 74 1322.5 75 1010.5 76 771.0 77 547.5 78 379.5 79 249.5 80 157.5 81 103.0 82 53.5 83 26.0 84 16.5 85 8.5 86 8.5 87 7.0 88 3.0 89 2.5 90 3.5 91 1.5 92 0.5 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38725289051868317 2 0.02957392767730073 3 0.035904241505043476 4 0.044214807350695495 5 0.06375762234711156 6 0.05466794095342967 7 0.038436367036140576 8 0.03989720868869659 9 0.03622887298338926 10-11 0.03793318824470461 12-13 0.029070748885864764 14-15 0.027691065102895193 16-17 0.0221723299710169 18-19 0.02700933899836905 20-21 0.03403761050455523 22-23 0.03340457912178095 24-25 0.0415203660804255 26-27 0.03611525196596823 28-29 0.031456790251706265 30-31 0.03428108411331456 32-33 0.028729885833601698 34-35 0.03046666424275163 36-37 0.03277154773900668 38-39 0.015241447908334458 40-41 0.012822943394658386 42-43 0.013553364220936394 44-45 0.012335996177139711 46-47 0.020906267205468353 48-49 0.01843906797004041 50-51 0.011865280533538327 52-53 0.010274586289643997 54-55 0.008894902506674423 56-57 0.009592860185117855 58-59 0.004155282922826008 60-61 0.007645071315043162 62-63 0.013098880151252297 64-65 0.008196944828230994 66-67 0.003863114592314804 68-69 0.006411471697329193 70-71 0.013163806446921455 72-73 0.010810228228914536 74-75 0.011394564889936945 76-77 0.005437577262291846 78-79 0.009803870646042613 80-81 0.01274178552507194 82-83 0.014072774586289645 84-85 0.009154607689351049 86-87 0.0025321255310970987 88-89 0.016036795030281624 90-91 0.02558096049364761 92-93 0.010826459802831825 94-95 0.020776414614130042 96-97 0.018666310004882457 98-99 0.011394564889936945 100-101 0.12168810965791632 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3080416.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.91649176086028 #Duplication Level Percentage of deduplicated Percentage of total 1 74.36947564872123 40.09741221073778 2 13.941676695176799 15.033725933361556 3 4.980520654154595 8.055966024435621 4 2.3356180516911924 5.037133257620989 5 1.2629067896987307 3.4045751760763054 6 0.7579495317045343 2.451958780877726 7 0.5239717614487659 1.9775503411353086 8 0.32958640915924364 1.421611433114071 9 0.2227064373347324 1.0806794814283782 >10 1.1683613377476474 11.057299678956541 >50 0.06871861357246238 2.5298053040340878 >100 0.033057910841105244 3.357206318154523 >500 0.0024828500974951834 0.9329193691167357 >1k 0.0028461940126979644 3.160524340305218 >5k 1.2111463883821125E-4 0.40163235064509434 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6879 0.22331399395406334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5422 0.17601518755908294 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4440 0.14413637638552715 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4290 0.1392669042103404 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4133 0.13417019000031166 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3779 0.12267823566687096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3612 0.11725688997849641 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3403 0.11047209208106958 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3307 0.10735562988895006 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3234 0.10498582009702585 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.246314783457819E-5 0.0 9 0.0 0.0 0.0 9.738944350373456E-5 0.0 10-11 0.0 0.0 0.0 9.738944350373456E-5 0.0 12-13 0.0 0.0 0.0 9.738944350373456E-5 3.246314783457819E-5 14-15 0.0 0.0 0.0 9.738944350373456E-5 6.492629566915638E-5 16-17 0.0 0.0 0.0 9.738944350373456E-5 9.738944350373456E-5 18-19 0.0 0.0 0.0 9.738944350373456E-5 9.738944350373456E-5 20-21 0.0 0.0 0.0 1.7854731309018004E-4 1.4608416525560184E-4 22-23 0.0 0.0 0.0 5.031787914359619E-4 1.6231573917289093E-4 24-25 3.246314783457819E-5 0.0 0.0 7.953471219471655E-4 1.7854731309018E-4 26-27 3.246314783457819E-5 0.0 0.0 0.0015419995221424639 1.9477888700746913E-4 28-29 6.492629566915638E-5 0.0 0.0 0.0042364407924124535 2.272420348420473E-4 30-31 6.492629566915638E-5 0.0 0.0 0.012709322377237361 2.272420348420473E-4 32-33 6.492629566915638E-5 0.0 0.0 0.031067232477691324 2.597051826766255E-4 34-35 6.492629566915638E-5 0.0 0.0 0.05874206600666923 2.597051826766255E-4 36-37 6.492629566915638E-5 0.0 0.0 0.10636550387999544 2.597051826766255E-4 38-39 6.492629566915638E-5 0.0 0.0 0.17762211337689454 2.597051826766255E-4 40-41 6.492629566915638E-5 0.0 0.0 0.25666987835409244 2.597051826766255E-4 42-43 6.492629566915638E-5 0.0 0.0 0.3381361478449664 2.597051826766255E-4 44-45 6.492629566915638E-5 0.0 0.0 0.4402165162107975 2.759367565939146E-4 46-47 9.738944350373456E-5 0.0 0.0 0.5630895307646759 2.921683305112037E-4 48-49 9.738944350373456E-5 0.0 0.0 0.6918059119287785 3.570946261803601E-4 50-51 9.738944350373456E-5 0.0 0.0 0.8331504576005319 3.8955777401493825E-4 52-53 1.1362101742102366E-4 0.0 0.0 0.9700962467406999 3.8955777401493825E-4 54-55 1.2985259133831275E-4 0.0 0.0 1.1209687263019021 3.8955777401493825E-4 56-57 1.2985259133831275E-4 0.0 0.0 1.2970001454349025 3.8955777401493825E-4 58-59 1.2985259133831275E-4 0.0 0.0 1.48478322408402 3.8955777401493825E-4 60-61 1.2985259133831275E-4 0.0 0.0 1.6947873274259062 3.8955777401493825E-4 62-63 1.2985259133831275E-4 0.0 0.0 1.8959127598350354 4.544840696840946E-4 64-65 1.2985259133831275E-4 0.0 0.0 2.1051377476288917 4.544840696840946E-4 66-67 1.2985259133831275E-4 0.0 0.0 2.3509973977540692 4.544840696840946E-4 68-69 1.4608416525560184E-4 0.0 0.0 2.620749924685497 4.544840696840946E-4 70-71 1.6231573917289093E-4 0.0 0.0 2.90082573262832 4.544840696840946E-4 72-73 1.6231573917289093E-4 0.0 0.0 3.1955424202445384 4.707156436013837E-4 74-75 1.6231573917289093E-4 0.0 0.0 3.4940897593052367 4.869472175186728E-4 76-77 1.6231573917289093E-4 0.0 0.0 3.8128454078929597 4.869472175186728E-4 78-79 1.6231573917289093E-4 0.0 0.0 4.164291446350104 4.869472175186728E-4 80-81 1.9477888700746913E-4 0.0 0.0 4.532861795289987 5.19410365353251E-4 82-83 1.9477888700746913E-4 0.0 0.0 4.911593109502093 5.19410365353251E-4 84-85 1.9477888700746913E-4 0.0 0.0 5.293067559706222 5.19410365353251E-4 86-87 1.9477888700746913E-4 0.0 0.0 5.6887933318097295 5.518735131878292E-4 88-89 1.9477888700746913E-4 3.246314783457819E-5 0.0 6.095475416307408 5.843366610224074E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4375 0.0 52.031513 1 GTATCAA 7970 0.0 41.91465 1 ATCAACG 9790 0.0 33.155922 3 TCAACGC 9970 0.0 32.55732 4 TATCAAC 10210 0.0 31.978207 2 CAACGCA 10275 0.0 31.637154 5 AACGCAG 10845 0.0 30.011827 6 TGGTATC 2075 0.0 26.33922 2 ACGCAGA 12285 0.0 26.294163 7 CGCAGAG 12460 0.0 25.81049 8 GTACATG 9020 0.0 24.443396 1 TACATGG 8890 0.0 23.896202 2 GTGGTAT 2470 0.0 23.764807 1 ACATGGG 8790 0.0 23.194849 3 GCAGAGT 13825 0.0 22.608526 9 TAAGGTG 2120 0.0 22.19333 5 GTAAGGT 2135 0.0 21.147005 4 GTGTAGC 1870 0.0 20.926584 1 CAGAGTA 13020 0.0 20.50275 10-11 AGAGTAC 12565 0.0 20.470234 10-11 >>END_MODULE