FastQCFastQC Report
Thu 26 May 2016
SRR938416_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938416_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080416
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70450.2287028764946033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59960.19464903441613082No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA44640.14491549193355704No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44050.14300016621131692No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41310.13410526370464249No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA40790.13241718001724442No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38580.12524282434580264No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA37840.12284055140604387No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37410.121444636049157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41450.055.480511
GTATCAA72750.040.3622251
ATCAACG93950.030.9409223
TCAACGC94550.030.6938424
CAACGCA97000.029.9185855
TATCAAC99150.029.4168782
AACGCAG102250.028.3824216
ACGCAGA117600.024.5969667
TGGTATC17100.024.4459822
CGCAGAG118500.024.1696598
TAAGGTG21100.023.4111375
GTACATG90450.022.9558031
TACATGG87200.022.9343552
ACATGGG85100.022.772423
GTAAGGT20100.022.4491124
GGTAAGG21350.020.9126263
GCAGAGT136600.020.5846089
AGAGTAC122450.020.22865910-11
GTGTAAG13700.020.1153261
GAGTACT87000.020.06363712-13
Produced by FastQC (version 0.11.5)