##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938416_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3080416 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.826950645627083 31.0 31.0 34.0 30.0 34.0 2 32.08307124751982 33.0 31.0 34.0 30.0 34.0 3 32.14279337595961 33.0 31.0 34.0 30.0 34.0 4 35.78931417055359 37.0 35.0 37.0 35.0 37.0 5 35.68258962425854 37.0 35.0 37.0 33.0 37.0 6 35.71481871279723 37.0 35.0 37.0 33.0 37.0 7 35.6401505510944 37.0 35.0 37.0 33.0 37.0 8 35.60848567206507 37.0 35.0 37.0 33.0 37.0 9 37.16679045947041 39.0 37.0 39.0 33.0 39.0 10-11 37.095959279525886 39.0 37.0 39.0 33.0 39.0 12-13 36.981776649647315 39.0 37.0 39.0 33.0 39.0 14-15 38.009665090689055 40.0 37.0 41.0 33.0 41.0 16-17 38.09190284688822 40.0 37.0 41.0 33.0 41.0 18-19 38.23001341377268 40.0 38.0 41.0 33.0 41.0 20-21 38.18528601331768 40.0 37.5 41.0 33.0 41.0 22-23 38.069771258167734 40.0 37.0 41.0 33.0 41.0 24-25 37.94454434076437 40.0 37.0 41.0 33.0 41.0 26-27 37.72893985747379 40.0 37.0 41.0 32.0 41.0 28-29 37.593455559249136 39.5 37.0 41.0 32.0 41.0 30-31 37.337738474283995 39.0 36.0 41.0 31.5 41.0 32-33 37.22545704865836 39.0 36.0 41.0 31.0 41.0 34-35 37.09659847890674 39.0 36.0 41.0 31.0 41.0 36-37 36.96266429599119 39.0 36.0 41.0 30.5 41.0 38-39 36.742315648276076 39.0 35.0 41.0 30.0 41.0 40-41 36.52083176428119 39.0 35.0 40.0 30.0 41.0 42-43 36.25847353084778 39.0 35.0 40.0 30.0 41.0 44-45 35.98161969032754 38.0 35.0 40.0 29.0 41.0 46-47 35.58961971370101 38.0 34.5 40.0 27.5 41.0 48-49 35.18753587177836 38.0 34.0 40.0 26.5 41.0 50-51 34.79202468108204 38.0 33.5 40.0 26.0 41.0 52-53 34.19567146125718 37.5 33.0 40.0 24.0 41.0 54-55 33.76682711036432 37.0 32.5 40.0 23.0 41.0 56-57 33.37375682375367 37.0 32.0 39.5 22.5 40.5 58-59 32.833711583110855 36.0 31.0 39.0 20.5 40.0 60-61 32.33621465412464 36.0 31.0 39.0 18.5 40.0 62-63 31.821233398346198 35.0 30.5 38.5 16.0 40.0 64-65 31.173784320039893 35.0 29.5 38.0 10.5 40.0 66-67 30.52028329939852 34.0 28.5 38.0 8.0 40.0 68-69 29.789750150629004 34.0 28.0 37.0 6.5 39.0 70-71 29.002600785088767 33.5 26.5 36.5 2.0 39.0 72-73 28.41058058392113 33.0 26.0 36.0 2.0 39.0 74-75 27.694286908002034 32.0 25.5 35.0 2.0 38.0 76-77 26.53262741136262 30.5 24.0 34.0 2.0 36.0 78-79 27.026117414011615 32.0 25.0 35.0 2.0 37.0 80-81 26.646285923719393 32.0 24.5 35.0 2.0 36.0 82-83 25.951285151096478 31.5 22.5 34.5 2.0 36.0 84-85 25.157975578623148 31.0 19.5 34.0 2.0 35.5 86-87 24.240368670984697 30.5 15.5 34.0 2.0 35.0 88-89 23.242359473525653 30.0 7.0 34.0 2.0 35.0 90-91 22.366857593260132 29.0 2.0 34.0 2.0 35.0 92-93 21.28172120259082 29.0 2.0 33.5 2.0 35.0 94-95 20.09425626279048 26.5 2.0 33.0 2.0 34.5 96-97 18.710487641928882 25.5 2.0 32.5 2.0 34.0 98-99 16.92251468632808 12.5 2.0 32.0 2.0 34.0 100-101 12.651227139451294 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2673603337264261 1101 2 0.2373312734262143 1101 3 0.23431538947757957 1101 4 0.19935676855534012 1101 5 0.10973236859010882 1101 6 0.1932558918816767 1101 7 0.24852011677273822 1101 8 0.17735103713749822 1101 9 0.2645316288410626 1101 10-11 0.3795342992702544 1101 12-13 0.3658022427533254 1101 14-15 0.44560579404719647 1101 16-17 0.4300788539553366 1101 18-19 0.3852826535723892 1101 20-21 0.38927389739404816 1101 22-23 0.3483384226882933 1101 24-25 0.3104959768186646 1101 26-27 0.5386812982240841 1101 28-29 0.46295683412412103 1101 30-31 0.519368330083978 1101 32-33 0.49802291582779645 1101 34-35 0.40369843080575407 1101 36-37 0.4666990728790026 1101 38-39 0.6053904614391357 1101 40-41 0.622326266721025 1101 42-43 0.7259354398825977 1101 44-45 0.7025505024950789 1101 46-47 0.8088062940274341 1101 48-49 0.9326667151703845 1101 50-51 0.9718757607366015 1101 52-53 1.0311752055500634 1101 54-55 1.0611200785887007 1101 56-57 1.2661304813470906 1101 58-59 1.3664310848139323 1101 60-61 1.4979221817879491 1101 62-63 1.6180436769029534 1101 64-65 1.5894510953310608 1101 66-67 1.6188640774782108 1101 68-69 1.7346322290591907 1101 70-71 1.8453659501616393 1101 72-73 1.9667565800500064 1101 74-75 1.9736428078874049 1101 76-77 1.762894768572071 1101 78-79 2.019909523048735 1101 80-81 2.256720499358586 1101 82-83 2.4874321804217168 1101 84-85 2.371319130417561 1101 86-87 2.57076486378131 1101 88-89 2.4658205821879413 1101 90-91 2.6901772936488406 1101 92-93 3.2446226163350147 1101 94-95 3.143952010022396 1101 96-97 3.2534433620347194 1101 98-99 3.09512766797506 1101 100-101 2.7679113372580133 1102 1 0.23842717352959752 1102 2 0.23072725483682177 1102 3 0.20857158594194658 1102 4 0.23217889357812282 1102 5 0.1774617744585285 1102 6 0.27320827934295266 1102 7 0.1906232696796124 1102 8 0.26635641348158856 1102 9 0.2921548746948943 1102 10-11 0.38750848826241935 1102 12-13 0.3491035686144457 1102 14-15 0.4943822201452477 1102 16-17 0.35654994471531865 1102 18-19 0.3549928777386029 1102 20-21 0.26473635410992813 1102 22-23 0.3567772326026315 1102 24-25 0.30752953340695655 1102 26-27 0.42532039973675495 1102 28-29 0.39099640492784005 1102 30-31 0.3393035178004027 1102 32-33 0.3562361926639497 1102 34-35 0.4512078849701524 1102 36-37 0.4838656712023166 1102 38-39 0.524454499164662 1102 40-41 0.6896237425730405 1102 42-43 0.6742841504712587 1102 44-45 0.7430210465082041 1102 46-47 0.6987586814886981 1102 48-49 0.8327840379332798 1102 50-51 0.806509098626158 1102 52-53 0.9220500005199952 1102 54-55 0.7498820386761835 1102 56-57 0.9341051259616293 1102 58-59 1.1166596933431876 1102 60-61 1.103768842075901 1102 62-63 1.2471235475062024 1102 64-65 1.2587635611593875 1102 66-67 1.2448773816517154 1102 68-69 1.4484735813464127 1102 70-71 1.445652650544357 1102 72-73 1.5724929797219573 1102 74-75 1.7476511457769632 1102 76-77 1.6299491698842665 1102 78-79 1.8233661022359051 1102 80-81 1.9654892937817685 1102 82-83 2.007091488786944 1102 84-85 2.0112444083472134 1102 86-87 1.951893668228184 1102 88-89 1.891404690079323 1102 90-91 2.0004741316295203 1102 92-93 2.090171413491934 1102 94-95 1.9466711643872614 1102 96-97 2.1587995448304156 1102 98-99 2.2971770574410684 1102 100-101 2.2017974544440904 1103 1 0.22810723259903298 1103 2 0.20941551367641864 1103 3 0.26124608782117775 1103 4 0.10945092941950207 1103 5 0.18552497431196713 1103 6 0.17600697137690702 1103 7 0.22339059495566715 1103 8 0.25271736991787463 1103 9 0.3021916820585915 1103 10-11 0.3352860563116238 1103 12-13 0.2742983478535379 1103 14-15 0.44782851081794206 1103 16-17 0.34434466732769664 1103 18-19 0.30100013928184666 1103 20-21 0.3791980949927449 1103 22-23 0.5321692615599787 1103 24-25 0.4547844146851716 1103 26-27 0.4880807299482157 1103 28-29 0.34730528699438423 1103 30-31 0.35409863457911683 1103 32-33 0.522769749543265 1103 34-35 0.573546845814441 1103 36-37 0.4912727228936191 1103 38-39 0.4639356985406593 1103 40-41 0.7052192390838314 1103 42-43 0.5990437367631571 1103 44-45 0.6496594738400248 1103 46-47 0.8154682808269484 1103 48-49 0.9512027092041109 1103 50-51 0.9031796144288933 1103 52-53 1.0485325378408774 1103 54-55 1.0621936854587304 1103 56-57 1.1847547787066333 1103 58-59 1.1791382778974153 1103 60-61 1.2310085673808189 1103 62-63 1.2240366143978925 1103 64-65 1.3776439622097492 1103 66-67 1.2690200143497563 1103 68-69 1.3653288556806338 1103 70-71 1.5405192169114237 1103 72-73 1.5111703573499646 1103 74-75 1.5424906756642116 1103 76-77 1.5823736259624788 1103 78-79 1.6238909541459456 1103 80-81 1.8370597412993526 1103 82-83 2.0928819033277968 1103 84-85 2.172390877227503 1103 86-87 2.1103298826768793 1103 88-89 1.911366827904299 1103 90-91 2.284146318475468 1103 92-93 2.409505716511468 1103 94-95 1.9740037130890116 1103 96-97 2.16763675413274 1103 98-99 2.4165641010269816 1103 100-101 2.0918520825675806 1104 1 0.12755092615422825 1104 2 0.20792231079283852 1104 3 0.19908720383921263 1104 4 0.12210529572544715 1104 5 0.0692437083503421 1104 6 0.07251602659926704 1104 7 0.17022324370390152 1104 8 0.05334236495029643 1104 9 0.135243170471675 1104 10-11 0.15822422737966946 1104 12-13 0.1974007524142607 1104 14-15 0.28298039751607007 1104 16-17 0.25461962456465415 1104 18-19 0.37127532032145183 1104 20-21 0.2118519676779016 1104 22-23 0.2600986604009776 1104 24-25 0.31257325919001744 1104 26-27 0.3922853541710296 1104 28-29 0.25053652658076686 1104 30-31 0.23860433819999116 1104 32-33 0.38631314571284747 1104 34-35 0.42862903785886175 1104 36-37 0.3131713620480383 1104 38-39 0.3161396735266351 1104 40-41 0.43118152771059925 1104 42-43 0.285874080553306 1104 44-45 0.3943357422676641 1104 46-47 0.44269605837465065 1104 48-49 0.5892514492370466 1104 50-51 0.5571215842853903 1104 52-53 0.6095647742065964 1104 54-55 0.6852941866511557 1104 56-57 0.7606543607604266 1104 58-59 0.8395963558970223 1104 60-61 0.8932012727532097 1104 62-63 0.8098656086790008 1104 64-65 0.9386430987916974 1104 66-67 0.9922882524874836 1104 68-69 1.0508864003606355 1104 70-71 1.1721301274367981 1104 72-73 1.444236058276843 1104 74-75 1.4316735931220315 1104 76-77 1.572644799470602 1104 78-79 1.5768439254711772 1104 80-81 1.793052663697562 1104 82-83 1.6596959869145316 1104 84-85 1.9403876427563738 1104 86-87 2.0412374567858826 1104 88-89 1.8561977502347418 1104 90-91 1.91966195767883 1104 92-93 2.18149563367939 1104 94-95 2.3056285642184164 1104 96-97 2.319597756835524 1104 98-99 2.192900913968547 1104 100-101 1.8979291296610956 1105 1 0.1708666313689129 1105 2 0.20611093166236572 1105 3 0.1937205209834545 1105 4 0.15193009381153644 1105 5 0.12938347414819162 1105 6 0.11752169278025804 1105 7 0.14540537095013661 1105 8 0.16914201598549994 1105 9 0.23831280154033863 1105 10-11 0.23399770605504244 1105 12-13 0.2481746807865406 1105 14-15 0.33741980535518934 1105 16-17 0.2676477935787389 1105 18-19 0.2591505923880817 1105 20-21 0.2541225772870277 1105 22-23 0.24919440986583652 1105 24-25 0.22102891008132275 1105 26-27 0.27117568209001064 1105 28-29 0.27063752850546763 1105 30-31 0.30280846461878497 1105 32-33 0.3560921646540862 1105 34-35 0.38780240500940266 1105 36-37 0.39485026047050553 1105 38-39 0.3866674378135073 1105 40-41 0.5495799771556733 1105 42-43 0.40140571223354726 1105 44-45 0.3934197096736298 1105 46-47 0.4321331277898608 1105 48-49 0.41382963354082847 1105 50-51 0.3758258276253841 1105 52-53 0.3847846759927904 1105 54-55 0.3724458963987871 1105 56-57 0.5477602014238201 1105 58-59 0.5744761968841985 1105 60-61 0.5491291773455202 1105 62-63 0.6268789916588666 1105 64-65 0.6450931014898806 1105 66-67 0.7390531329580448 1105 68-69 0.8451810957359491 1105 70-71 0.9642991055455532 1105 72-73 1.1386296869711003 1105 74-75 0.9699099718027959 1105 76-77 1.0009891953477812 1105 78-79 1.1004786456697637 1105 80-81 1.3397820181126896 1105 82-83 1.45453346645332 1105 84-85 1.7946822481923093 1105 86-87 1.748936717484174 1105 88-89 1.83571810351663 1105 90-91 1.6243981654747763 1105 92-93 1.9160166165369432 1105 94-95 1.8273610665118731 1105 96-97 2.055106274094893 1105 98-99 2.001646169666209 1105 100-101 1.9198900779819255 1106 1 0.0057843612257677535 1106 2 0.09583711045515031 1106 3 0.1118358671723314 1106 4 0.11379847926550468 1106 5 0.08022385857654513 1106 6 -0.012554432521454828 1106 7 0.05692584343981366 1106 8 0.017555409087364637 1106 9 -0.03829082524232774 1106 10-11 0.04521093647333885 1106 12-13 0.08390582850145023 1106 14-15 0.09578209459597531 1106 16-17 0.029494075286564225 1106 18-19 0.1061228212414278 1106 20-21 0.09750471569184782 1106 22-23 0.15028633492529053 1106 24-25 0.10757347565429143 1106 26-27 -0.1625829273191144 1106 28-29 0.06422302137479363 1106 30-31 0.2126556835254405 1106 32-33 0.053480386009837844 1106 34-35 -0.016494563240776472 1106 36-37 0.06571752305416823 1106 38-39 0.25946391536052005 1106 40-41 0.24739524288690973 1106 42-43 0.3370462345892804 1106 44-45 0.2636048506513262 1106 46-47 -0.030086265152860392 1106 48-49 0.1864249669979401 1106 50-51 0.5458248550605518 1106 52-53 0.28396462701088865 1106 54-55 0.3442574310176738 1106 56-57 0.6580097085102778 1106 58-59 0.7823659080324532 1106 60-61 0.9046414095586357 1106 62-63 0.7170105531559123 1106 64-65 0.9882864739026367 1106 66-67 1.0552414745581835 1106 68-69 0.9363957407939942 1106 70-71 0.9120807735826482 1106 72-73 1.1497120632122275 1106 74-75 0.954047881495736 1106 76-77 0.9788129489175361 1106 78-79 1.2022173263413656 1106 80-81 1.1904296578746774 1106 82-83 1.608020553444824 1106 84-85 1.753849555867614 1106 86-87 1.6973487843612673 1106 88-89 1.734141664341717 1106 90-91 1.8116354624518713 1106 92-93 1.8546535227198078 1106 94-95 1.8127854142303228 1106 96-97 1.9561190677431597 1106 98-99 1.997566260203154 1106 100-101 1.8644339892840147 1107 1 -0.02843411551297237 1107 2 0.022510548919306927 1107 3 0.04123052611746658 1107 4 0.01817814119859662 1107 5 -0.03238409080369564 1107 6 -0.08197432729138399 1107 7 -0.04589888171358325 1107 8 -0.022278429935866484 1107 9 -0.08733969116665463 1107 10-11 0.013080064806722191 1107 12-13 -0.02632979684101855 1107 14-15 0.07523704696912858 1107 16-17 0.03144726552272914 1107 18-19 0.009070156392148476 1107 20-21 0.09223762954782444 1107 22-23 0.09608762007808735 1107 24-25 0.1381799294368662 1107 26-27 0.08495776311030312 1107 28-29 -0.01611486994320188 1107 30-31 0.13123708165731784 1107 32-33 0.15111212734092305 1107 34-35 0.19061221682105156 1107 36-37 0.22220368543568725 1107 38-39 0.28896532363864225 1107 40-41 0.30764534514143094 1107 42-43 0.27320510424114985 1107 44-45 0.28833789620235706 1107 46-47 0.4265526634831218 1107 48-49 0.19329854185235718 1107 50-51 0.3142911250006577 1107 52-53 0.25914674312889474 1107 54-55 0.37215855361297656 1107 56-57 0.2992901031068129 1107 58-59 0.4995077075250194 1107 60-61 0.6504526004693147 1107 62-63 0.6945469568161222 1107 64-65 0.8360520574825152 1107 66-67 0.8937151614106433 1107 68-69 1.1037424024295284 1107 70-71 0.8943202173180431 1107 72-73 1.0855066719987292 1107 74-75 0.8947247501387032 1107 76-77 1.0236043430088593 1107 78-79 1.191873968724611 1107 80-81 1.2376281129581734 1107 82-83 1.4385229681403082 1107 84-85 1.5492256091655534 1107 86-87 1.9002748669852103 1107 88-89 1.9643461333081653 1107 90-91 2.000599150310176 1107 92-93 2.1243969006061434 1107 94-95 1.761321900533943 1107 96-97 1.6901890249155471 1107 98-99 1.8111413760995667 1107 100-101 1.5721218021671106 1108 1 -0.05291106591243988 1108 2 0.0022972950942659054 1108 3 0.046344349346135516 1108 4 0.007892629827772168 1108 5 -0.01832273283653052 1108 6 -0.013374966381299203 1108 7 0.009904789718582663 1108 8 5.772909399794912E-4 1108 9 0.023571064853619816 1108 10-11 0.013581208075741813 1108 12-13 -0.026908123993059974 1108 14-15 0.08011108498793362 1108 16-17 0.09484067011852915 1108 18-19 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-0.7982670732371986 2316 48-49 -0.45346907501553346 2316 50-51 -0.6542638583862512 2316 52-53 -0.49035850242746193 2316 54-55 -0.32101480581822983 2316 56-57 -0.15556702930227573 2316 58-59 -0.24239144833408233 2316 60-61 -0.03537592449234239 2316 62-63 -0.14879552107850103 2316 64-65 -0.24611502070197488 2316 66-67 -0.11825752855875749 2316 68-69 0.17257636907745422 2316 70-71 0.08883915426939026 2316 72-73 0.47587009573906514 2316 74-75 0.4750121007298347 2316 76-77 0.5643204330322042 2316 78-79 0.36243626311099675 2316 80-81 0.09649605032553055 2316 82-83 0.36228625167744255 2316 84-85 0.5538398458412992 2316 86-87 0.7149122566044106 2316 88-89 0.736431012228806 2316 90-91 0.8773712703435699 2316 92-93 0.914108072207668 2316 94-95 1.1188151848985726 2316 96-97 1.0881733824001607 2316 98-99 0.9337415178107342 2316 100-101 0.346241116637918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 22.0 4 47.0 5 93.0 6 208.0 7 557.0 8 1411.0 9 2534.0 10 4205.0 11 6475.0 12 9717.0 13 13049.0 14 17845.0 15 23694.0 16 29838.0 17 36626.0 18 37405.0 19 35728.0 20 37612.0 21 40590.0 22 44847.0 23 50131.0 24 56600.0 25 64543.0 26 74267.0 27 86419.0 28 101928.0 29 119667.0 30 140089.0 31 162900.0 32 191823.0 33 228034.0 34 275847.0 35 336522.0 36 388421.0 37 343740.0 38 114694.0 39 2277.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.89680735203293 19.40822265474059 15.089241008196419 23.60572898503006 2 15.79015036429568 20.426823824135493 39.47435320106505 24.30867261050378 3 19.208864940742295 23.51083104274534 30.29416376467808 26.986140251834286 4 13.237887350279962 17.06415626980252 36.75919096641492 32.938765413502594 5 13.636157052024961 37.38977913950304 34.71464475786633 14.259419050605668 6 32.7878117760718 36.53665608800889 16.912455979971536 13.763076155947768 7 28.81659490146785 32.54872069227013 20.361275879621456 18.27340852664056 8 26.440935030465067 35.57870324531159 19.43210212396844 18.5482596002549 9 26.013883221351453 16.699990163643854 19.806850324096573 37.479276290908125 10-11 24.862372484755305 26.519242855510424 27.65553418759025 20.962850472144023 12-13 25.79998935208751 23.624942215596857 27.846109096953136 22.728959335362497 14-15 22.24300224385278 25.440946937037072 25.87251202434996 26.44353879476019 16-17 22.124008575465133 28.56911209395095 26.93556649491497 22.37131283566895 18-19 22.201595498789775 27.04586003968295 28.96228950894944 21.790254952577833 20-21 22.487190041864473 26.342724489159906 29.300506814663997 21.869578654311624 22-23 22.16945698243354 26.28821886394565 29.197501246584878 22.344822907035933 24-25 22.175982963002642 26.512350037576837 29.228676564876448 22.08299043454407 26-27 21.824528094224995 26.899935943991736 28.905541575206396 22.369994386576874 28-29 21.96816689469371 27.289342537088007 28.715613946782074 22.02687662143621 30-31 22.182539631010876 26.78610616228457 28.660090065757355 22.371264140947197 32-33 21.731666714279406 26.79408052439807 28.710563665592346 22.763689095730175 34-35 22.514837180611018 26.98211097042304 28.49413980065582 22.00891204831012 36-37 22.67076400064018 26.454261929627442 28.561617620180908 22.313356449551467 38-39 22.12359513351913 26.804021288256397 28.535380427583306 22.537003150641166 40-41 22.681099038139518 26.61550466953061 28.58250456449884 22.120891727831033 42-43 22.36100219608406 26.673485365093903 28.386817262399173 22.578695176422865 44-45 22.29613958223499 26.909399842617432 28.652343597827805 22.14211697731977 46-47 22.685975122808248 27.12187525564762 27.879225246493 22.31292437505113 48-49 22.404538867477637 27.262356772591755 27.777514465578673 22.555589894351932 50-51 22.617821748750817 27.30080937120181 27.838593878229435 22.242775001817936 52-53 22.930555483415226 26.985754521467232 27.77527450837809 22.308415486739452 54-55 22.259558449248413 27.219846280502374 28.05056200201531 22.4700332682339 56-57 22.42780599367293 27.335943371266534 27.74755998785878 22.488690647201757 58-59 22.318397904698585 26.979359281343818 27.81778500046747 22.884457813490126 60-61 22.036504160477026 27.200904033740898 28.110505204491858 22.65208660129022 62-63 22.218135472611493 27.15441680604178 28.166682681819598 22.46076503952713 64-65 21.863110865833715 27.238998023803628 28.001623159235976 22.896267951126674 66-67 21.76235162010852 27.306915621711564 27.950534273926834 22.98019848425308 68-69 22.421210728725587 26.905115254280755 28.02888730286755 22.644786714126113 70-71 22.193501325958565 26.94262100568982 27.654278825599032 23.20959884275258 72-73 22.154938566579492 27.102822093696677 27.64750643421261 23.09473290551122 74-75 22.19299276461361 27.365200024931696 27.44984768291036 22.99195952754433 76-77 22.167785130320063 27.015490764883705 27.843852908178633 22.9728711966176 78-79 22.227923111683616 27.220008596241545 27.655810124346843 22.896258167727996 80-81 22.4367280561522 27.262184119112582 27.390575972998434 22.910511851736786 82-83 22.342728717060048 27.31762140880113 27.532335317288982 22.807314556849843 84-85 22.225454295178764 27.441852311111624 27.44860503496726 22.88408835874235 86-87 22.411713421650617 27.37883427093277 27.14307766900935 23.066374638407254 88-89 22.15497841849932 27.49396510081755 26.930031528209174 23.42102495247395 90-91 22.076634076521103 27.422875561740938 27.178149182292177 23.322341179445775 92-93 22.20682739365462 27.05264365872024 27.401997695428793 23.338531252196347 94-95 21.883716355193584 26.915309490666196 27.461566879278642 23.739407274861577 96-97 22.170697779357653 26.572669591597236 27.478732573370795 23.777900055674316 98-99 21.856739479343048 26.88880333045926 27.068405695854064 24.186051494343623 100-101 21.970767885545236 26.3981767382853 27.558459278275464 24.072596097894 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 278.0 1 197.0 2 169.5 3 220.0 4 191.0 5 196.0 6 364.0 7 812.5 8 1320.0 9 1535.0 10 1384.5 11 1242.5 12 1334.5 13 1518.5 14 1726.5 15 1962.5 16 2250.0 17 2541.0 18 2864.0 19 3307.0 20 3973.0 21 4893.0 22 6000.0 23 7552.0 24 9818.0 25 12872.5 26 16971.0 27 21691.5 28 26163.0 29 30829.5 30 36162.0 31 43728.5 32 55104.5 33 60149.0 34 60765.0 35 65759.0 36 72012.0 37 78125.5 38 83543.0 39 88332.0 40 93036.5 41 98396.5 42 103294.5 43 107407.5 44 111084.0 45 115479.0 46 117271.5 47 118517.0 48 119397.5 49 116500.0 50 112442.0 51 108929.5 52 106105.5 53 105297.0 54 104819.5 55 97342.5 56 87424.0 57 79410.5 58 72812.0 59 63866.0 60 51274.5 61 42339.0 62 36577.5 63 28023.0 64 19098.5 65 13755.5 66 10193.5 67 7785.5 68 5881.5 69 4324.0 70 3322.5 71 2438.0 72 1799.0 73 1452.0 74 1118.0 75 816.5 76 575.5 77 412.5 78 284.5 79 177.0 80 102.0 81 55.5 82 30.5 83 15.0 84 6.0 85 2.5 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02525632901530183 2 0.011784122663951882 3 0.001850399426570957 4 0.0 5 3.246314783457819E-4 6 0.0 7 0.0 8 9.738944350373456E-5 9 2.2724203484204733E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001980252017909269 26-27 0.009706481202538877 28-29 2.1101046092475821E-4 30-31 0.0 32-33 2.597051826766255E-4 34-35 0.010258354715726708 36-37 0.0012660627655485493 38-39 0.013244964316507901 40-41 0.012839174968575673 42-43 0.0052103352274497985 44-45 7.791155480298765E-4 46-47 1.2985259133831275E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.246314783457819E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.1362101742102366E-4 66-67 6.654945306088528E-4 68-69 0.0010225891567892129 70-71 9.738944350373456E-5 72-73 2.597051826766255E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.115786958644548E-5 82-83 0.001233599617713971 84-85 0.005762208740637628 86-87 0.003911809314066672 88-89 0.0 90-91 0.001201136469879393 92-93 5.3564193927054E-4 94-95 0.0 96-97 3.246314783457819E-5 98-99 0.0 100-101 8.115786958644548E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3080416.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.894825979608626 #Duplication Level Percentage of deduplicated Percentage of total 1 71.7985079685963 36.54172568657251 2 14.984210535068993 15.252375752483092 3 5.555264758432193 8.482026994531768 4 2.676553407741974 5.448908796486249 5 1.445424799383165 3.678232181560844 6 0.8657385431157698 2.6436967497430204 7 0.5670824412838437 2.0203093515662993 8 0.37575804855147604 1.529931239317176 9 0.28558094654801136 1.3081133319887657 >10 1.3212482303932185 11.824643177607493 >50 0.07934656926716167 2.7557302127456684 >100 0.03908194677070038 3.729500346031033 >500 0.002877125960453938 0.9901652391260812 >1k 0.0031968066218561447 3.3702844092774233 >5k 1.2787226487424579E-4 0.4243565309625759 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7045 0.2287028764946033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5996 0.19464903441613082 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4464 0.14491549193355704 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4405 0.14300016621131692 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4131 0.13410526370464249 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4079 0.13241718001724442 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3858 0.12524282434580264 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3784 0.12284055140604387 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3741 0.121444636049157 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.246314783457819E-5 2 0.0 0.0 0.0 0.0 3.246314783457819E-5 3 0.0 0.0 0.0 0.0 3.246314783457819E-5 4 0.0 0.0 0.0 0.0 3.246314783457819E-5 5 0.0 0.0 0.0 0.0 3.246314783457819E-5 6 3.246314783457819E-5 0.0 0.0 0.0 3.246314783457819E-5 7 3.246314783457819E-5 0.0 0.0 0.0 3.246314783457819E-5 8 3.246314783457819E-5 0.0 0.0 0.0 3.246314783457819E-5 9 3.246314783457819E-5 0.0 0.0 6.492629566915638E-5 3.246314783457819E-5 10-11 3.246314783457819E-5 0.0 0.0 6.492629566915638E-5 3.246314783457819E-5 12-13 3.246314783457819E-5 0.0 0.0 6.492629566915638E-5 9.738944350373456E-5 14-15 3.246314783457819E-5 0.0 0.0 6.492629566915638E-5 9.738944350373456E-5 16-17 3.246314783457819E-5 0.0 0.0 6.492629566915638E-5 1.2985259133831275E-4 18-19 3.246314783457819E-5 0.0 0.0 6.492629566915638E-5 1.2985259133831275E-4 20-21 3.246314783457819E-5 0.0 0.0 1.4608416525560184E-4 1.2985259133831275E-4 22-23 3.246314783457819E-5 0.0 0.0 5.031787914359619E-4 1.2985259133831275E-4 24-25 3.246314783457819E-5 0.0 0.0 7.953471219471655E-4 1.2985259133831275E-4 26-27 3.246314783457819E-5 0.0 0.0 0.0015257679482251747 1.2985259133831275E-4 28-29 3.246314783457819E-5 0.0 0.0 0.004187746070660586 1.6231573917289093E-4 30-31 3.246314783457819E-5 0.0 0.0 0.012660627655485493 1.9477888700746913E-4 32-33 3.246314783457819E-5 0.0 0.0 0.031083464051608615 2.272420348420473E-4 34-35 3.246314783457819E-5 0.0 0.0 0.058677139711000074 2.597051826766255E-4 36-37 3.246314783457819E-5 0.0 0.0 0.10664144063658934 2.597051826766255E-4 38-39 3.246314783457819E-5 0.0 0.0 0.17854731309018002 3.0839990442849276E-4 40-41 3.246314783457819E-5 0.0 0.0 0.2580982568588139 3.2463147834578185E-4 42-43 3.246314783457819E-5 0.0 0.0 0.3397593052366953 3.40863052263071E-4 44-45 6.492629566915638E-5 0.0 0.0 0.44252139970705257 3.7332620009764917E-4 46-47 6.492629566915638E-5 0.0 0.0 0.565232098521758 3.8955777401493825E-4 48-49 6.492629566915638E-5 0.0 0.0 0.6936076166335976 3.8955777401493825E-4 50-51 6.492629566915638E-5 0.0 0.0 0.8349196991575164 3.8955777401493825E-4 52-53 6.492629566915638E-5 0.0 0.0 0.9721089619064438 4.2202092184951643E-4 54-55 6.492629566915638E-5 0.0 0.0 1.123030136189398 4.2202092184951643E-4 56-57 6.492629566915638E-5 0.0 0.0 1.2993537236529091 4.2202092184951643E-4 58-59 6.492629566915638E-5 0.0 0.0 1.4874614337803727 4.2202092184951643E-4 60-61 6.492629566915638E-5 0.0 0.0 1.697027284626492 4.2202092184951643E-4 62-63 6.492629566915638E-5 0.0 0.0 1.897974169722531 4.2202092184951643E-4 64-65 6.492629566915638E-5 0.0 0.0 2.1073127785338084 4.2202092184951643E-4 66-67 9.738944350373456E-5 1.6231573917289094E-5 0.0 2.3542924072592797 4.382524957668055E-4 68-69 9.738944350373456E-5 3.246314783457819E-5 0.0 2.6266257544435554 4.544840696840946E-4 70-71 1.2985259133831275E-4 3.246314783457819E-5 0.0 2.91334027611855 4.544840696840946E-4 72-73 1.2985259133831275E-4 3.246314783457819E-5 0.0 3.2149878457974506 4.707156436013837E-4 74-75 1.2985259133831275E-4 3.246314783457819E-5 0.0 3.521001708860102 5.031787914359619E-4 76-77 1.2985259133831275E-4 3.246314783457819E-5 0.0 3.851249311781266 5.19410365353251E-4 78-79 1.2985259133831275E-4 3.246314783457819E-5 0.0 4.216297409181097 5.19410365353251E-4 80-81 1.2985259133831275E-4 3.246314783457819E-5 0.0 4.602268005360315 5.19410365353251E-4 82-83 1.2985259133831275E-4 3.246314783457819E-5 0.0 5.000882997621101 5.518735131878292E-4 84-85 1.2985259133831275E-4 3.246314783457819E-5 0.0 5.4065100298141555 5.518735131878292E-4 86-87 1.2985259133831275E-4 3.246314783457819E-5 0.0 5.828709498976762 5.518735131878292E-4 88-89 1.2985259133831275E-4 3.246314783457819E-5 3.246314783457819E-5 6.270938730353303 5.518735131878292E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4145 0.0 55.48051 1 GTATCAA 7275 0.0 40.362225 1 ATCAACG 9395 0.0 30.940922 3 TCAACGC 9455 0.0 30.693842 4 CAACGCA 9700 0.0 29.918585 5 TATCAAC 9915 0.0 29.416878 2 AACGCAG 10225 0.0 28.382421 6 ACGCAGA 11760 0.0 24.596966 7 TGGTATC 1710 0.0 24.445982 2 CGCAGAG 11850 0.0 24.169659 8 TAAGGTG 2110 0.0 23.411137 5 GTACATG 9045 0.0 22.955803 1 TACATGG 8720 0.0 22.934355 2 ACATGGG 8510 0.0 22.77242 3 GTAAGGT 2010 0.0 22.449112 4 GGTAAGG 2135 0.0 20.912626 3 GCAGAGT 13660 0.0 20.584608 9 AGAGTAC 12245 0.0 20.228659 10-11 GTGTAAG 1370 0.0 20.115326 1 GAGTACT 8700 0.0 20.063637 12-13 >>END_MODULE