FastQCFastQC Report
Thu 26 May 2016
SRR938415_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938415_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178748
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82640.7010828438309121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65250.5535534312677519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48790.41391374577093665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39380.33408328158351064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.12063647191766179No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13430.11393444570001392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48100.033.5114441
TATCGCG600.00394350731.6789367
GTATCAA71100.030.5622331
TCAACGC86100.025.1284224
CAACGCA86600.024.928435
ATCAACG87250.024.7972153
TATCAAC87300.024.7830122
AACGCAG88450.024.4968596
CATTCCG2751.8189894E-1224.1911879
GTACATG52650.023.9323861
TAGGACG2653.092282E-1123.326764
TACATGG53900.022.849042
GTATAGC7550.022.672221
ACATGGG52600.022.3289453
ACGCAGA99550.021.6708747
CGCAGAG99450.021.6448848
AGTACTT57850.021.43781912-13
GAGTACT57650.021.34734512-13
GTGTAGC8300.021.1964021
CATGGGG20450.020.4619164
Produced by FastQC (version 0.11.5)