##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938415_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1178748 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.335285404513943 31.0 31.0 34.0 30.0 34.0 2 31.381265546155753 31.0 31.0 34.0 28.0 34.0 3 31.412201759833316 31.0 31.0 34.0 28.0 34.0 4 35.06287942800327 37.0 35.0 37.0 32.0 37.0 5 35.075161951494294 37.0 35.0 37.0 32.0 37.0 6 35.10786699107867 37.0 35.0 37.0 32.0 37.0 7 35.04617696063959 37.0 35.0 37.0 32.0 37.0 8 35.03566835320187 37.0 35.0 37.0 32.0 37.0 9 36.560824705535026 39.0 37.0 39.0 32.0 39.0 10-11 36.43257676789271 39.0 37.0 39.0 32.0 39.0 12-13 36.34094564741573 39.0 36.0 39.0 32.0 39.0 14-15 37.356080773838 40.0 37.0 41.0 32.0 41.0 16-17 37.22607588729737 39.0 36.5 41.0 32.0 41.0 18-19 37.40410376093958 39.5 37.0 41.0 32.0 41.0 20-21 37.42851652770567 40.0 37.0 41.0 32.0 41.0 22-23 37.33376514742761 40.0 37.0 41.0 32.0 41.0 24-25 37.2424110157557 40.0 36.5 41.0 31.5 41.0 26-27 37.06763277647131 39.0 36.0 41.0 31.0 41.0 28-29 36.887153997292046 39.0 36.0 41.0 31.0 41.0 30-31 36.65202189102335 39.0 36.0 41.0 30.0 41.0 32-33 36.380176254805946 39.0 35.0 40.0 30.0 41.0 34-35 36.15480535279805 38.5 35.0 40.0 30.0 41.0 36-37 35.91840240662126 38.0 35.0 40.0 29.5 41.0 38-39 35.778464099196775 38.0 35.0 40.0 29.0 41.0 40-41 35.690168721389135 38.0 35.0 40.0 28.0 41.0 42-43 35.43656320095559 38.0 35.0 40.0 27.0 41.0 44-45 35.07164423608778 38.0 34.5 40.0 25.5 41.0 46-47 34.672751936800736 38.0 34.0 40.0 24.0 41.0 48-49 34.33902114786197 38.0 33.5 40.0 23.0 41.0 50-51 33.67049869861921 37.5 33.0 39.5 22.0 40.5 52-53 33.74362247062137 38.0 33.0 39.5 22.0 40.5 54-55 33.929358946950494 38.0 33.0 40.0 20.5 41.0 56-57 33.697208393990906 38.0 33.0 40.0 18.5 41.0 58-59 33.43163000064475 38.0 33.0 40.0 17.5 41.0 60-61 33.07612017157187 38.0 32.5 40.0 13.5 41.0 62-63 32.62242184080058 37.0 31.5 40.0 8.5 41.0 64-65 32.21187734783007 36.5 31.0 39.0 8.0 41.0 66-67 31.780804294047584 36.0 31.0 39.0 4.5 40.0 68-69 31.28565690037226 35.5 30.5 39.0 2.0 40.0 70-71 30.757892272139593 35.0 29.5 38.0 2.0 40.0 72-73 30.165587131430975 35.0 29.0 38.0 2.0 39.5 74-75 29.538168039309504 34.0 28.0 37.0 2.0 39.0 76-77 28.841831332905763 34.0 27.5 36.5 2.0 39.0 78-79 28.107769429937527 34.0 26.0 36.0 2.0 38.5 80-81 27.426776545962326 33.0 26.0 35.5 2.0 37.0 82-83 26.721838340340767 32.5 24.5 35.0 2.0 37.0 84-85 26.037431240604437 32.0 22.5 35.0 2.0 36.0 86-87 25.32191655892523 31.5 19.5 34.5 2.0 36.0 88-89 24.546167628704353 31.0 15.5 34.0 2.0 35.0 90-91 23.69341496231595 31.0 4.5 34.0 2.0 35.0 92-93 22.830982958189537 30.0 2.0 34.0 2.0 35.0 94-95 21.89355867411864 29.5 2.0 34.0 2.0 35.0 96-97 20.697465870567754 29.0 2.0 34.0 2.0 35.0 98-99 19.24809628521109 26.5 2.0 34.0 2.0 35.0 100-101 15.48650093149681 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32546219941157517 1101 2 0.272031159392931 1101 3 0.31029354091089445 1101 4 0.17824378555968678 1101 5 0.10192155745207998 1101 6 0.26592227924669487 1101 7 0.18909415164203836 1101 8 0.2773692891257511 1101 9 0.4027833774383893 1101 10-11 0.45456138408876967 1101 12-13 0.40587415292796436 1101 14-15 0.4900752090856173 1101 16-17 0.4932559563134973 1101 18-19 0.5161453818364308 1101 20-21 0.6209227152883443 1101 22-23 0.5229027229087748 1101 24-25 0.32503119939555347 1101 26-27 0.3111454954512922 1101 28-29 0.46085497091760175 1101 30-31 0.46995257658635126 1101 32-33 0.5000994574921052 1101 34-35 0.47031736112634803 1101 36-37 0.40437006651275453 1101 38-39 0.5054182171928616 1101 40-41 0.6581672873119331 1101 42-43 0.949969658472753 1101 44-45 0.9693715532705767 1101 46-47 0.7927700406499767 1101 48-49 0.5164766360306956 1101 50-51 0.6081103298805388 1101 52-53 0.610033922459678 1101 54-55 0.7116214015069531 1101 56-57 0.6304111931308611 1101 58-59 0.6364793481238706 1101 60-61 0.7958774376038846 1101 62-63 0.8747311647836824 1101 64-65 0.6037901538144226 1101 66-67 0.6543386256742956 1101 68-69 0.8610257066732245 1101 70-71 0.6622184458500016 1101 72-73 0.8066214538125571 1101 74-75 0.3213342272287001 1101 76-77 0.5362623421996418 1101 78-79 0.6652465210291361 1101 80-81 0.8608971495347326 1101 82-83 0.8796216674177337 1101 84-85 0.7497463263329465 1101 86-87 0.8570092682167925 1101 88-89 0.5458302519136957 1101 90-91 0.35127884101874685 1101 92-93 0.34758844418671586 1101 94-95 0.535434446579707 1101 96-97 0.48815186788411324 1101 98-99 0.6539802855768535 1101 100-101 0.8237219579403501 1102 1 0.47762919055074704 1102 2 0.3491030831206636 1102 3 0.32625758814438655 1102 4 0.23024611448383325 1102 5 0.24970726004119115 1102 6 0.4331917976324604 1102 7 0.36973315020465236 1102 8 0.4871944378675863 1102 9 0.675696897934742 1102 10-11 0.6073451762322932 1102 12-13 0.7255289990916083 1102 14-15 0.7105515468524004 1102 16-17 0.926284831333966 1102 18-19 0.7091822625336519 1102 20-21 0.7379275004996728 1102 22-23 0.8918174988408012 1102 24-25 0.7721732637684724 1102 26-27 0.8399249936409134 1102 28-29 0.6066556295664682 1102 30-31 0.72022049383677 1102 32-33 0.794404055297818 1102 34-35 0.7017487397402817 1102 36-37 0.71277056868702 1102 38-39 0.7633870496376787 1102 40-41 0.8213798380297206 1102 42-43 0.9050396171707433 1102 44-45 0.9916914710304425 1102 46-47 0.9294474118768434 1102 48-49 1.0089772273590967 1102 50-51 0.9155342304645941 1102 52-53 0.8520732776388087 1102 54-55 0.9126348474049522 1102 56-57 1.201862313015873 1102 58-59 1.1061592120273644 1102 60-61 1.1180540719445986 1102 62-63 1.046756328081294 1102 64-65 1.127117604188328 1102 66-67 0.9160987828766771 1102 68-69 0.9391360940388154 1102 70-71 0.9106332308793874 1102 72-73 0.784053178126161 1102 74-75 1.0963715264044787 1102 76-77 1.0904307525269665 1102 78-79 1.0616958214421572 1102 80-81 1.3129126696307978 1102 82-83 1.6125204501909742 1102 84-85 1.390252776361148 1102 86-87 1.3852365566576843 1102 88-89 1.5163593543681664 1102 90-91 1.291239904318175 1102 92-93 1.5943479645738954 1102 94-95 1.6140388205903697 1102 96-97 1.2454902532846717 1102 98-99 1.3690004817159327 1102 100-101 1.5948069090875272 1103 1 0.2879723989516698 1103 2 0.3206774022532528 1103 3 0.22116068726502291 1103 4 0.37609260983088433 1103 5 0.3192496488399641 1103 6 0.3630219009364808 1103 7 0.23042639702443068 1103 8 0.2842233800685605 1103 9 0.3999349240595578 1103 10-11 0.41224998285741066 1103 12-13 0.5124537835191632 1103 14-15 0.5049850822404238 1103 16-17 0.6129577588503992 1103 18-19 0.578848445407381 1103 20-21 0.49978185036734857 1103 22-23 0.5921483278342237 1103 24-25 0.6657551812960065 1103 26-27 0.4223761163251112 1103 28-29 0.6219335259209444 1103 30-31 0.7432483261598293 1103 32-33 0.5949440535903108 1103 34-35 0.7188152470398563 1103 36-37 0.7723027124588953 1103 38-39 0.9403933674042619 1103 40-41 0.9639457903903406 1103 42-43 0.954984123275068 1103 44-45 0.9535863741295572 1103 46-47 0.6657468227878027 1103 48-49 0.8459254899732898 1103 50-51 0.9179909025383566 1103 52-53 0.9924536242622111 1103 54-55 1.0422942002607627 1103 56-57 0.9571747863361182 1103 58-59 0.7742139375460155 1103 60-61 0.9202524839654345 1103 62-63 0.919156652507148 1103 64-65 0.9717005462185497 1103 66-67 1.0094309778736879 1103 68-69 1.2954816075707853 1103 70-71 1.450364725799556 1103 72-73 1.3176888820448873 1103 74-75 1.3344638170217458 1103 76-77 1.2450154020616715 1103 78-79 1.1842732994580345 1103 80-81 1.3497926320657 1103 82-83 1.3798516206852973 1103 84-85 1.6335828369775598 1103 86-87 1.68550121985243 1103 88-89 1.8375964413655161 1103 90-91 1.7016386066147682 1103 92-93 1.6235249474134754 1103 94-95 1.5376301293925536 1103 96-97 1.7086102908497693 1103 98-99 1.969134947693906 1103 100-101 1.9349674145088542 1104 1 0.38227908572894265 1104 2 0.2644280307097624 1104 3 0.2424336887733176 1104 4 0.33781005578362056 1104 5 0.35647036276655797 1104 6 0.4745754454311992 1104 7 0.4858673221124121 1104 8 0.4791273519137107 1104 9 0.5753195394441732 1104 10-11 0.7166743166935134 1104 12-13 0.5649042611461184 1104 14-15 0.5729750269362555 1104 16-17 0.7230311625708623 1104 18-19 0.6092074197663564 1104 20-21 0.5950176472502022 1104 22-23 0.601274521901594 1104 24-25 0.49946257194457644 1104 26-27 0.6534470062195297 1104 28-29 0.6804795289375392 1104 30-31 0.7599039319919001 1104 32-33 0.7809973467024349 1104 34-35 0.590604085652231 1104 36-37 0.7328376546409032 1104 38-39 0.80498663035047 1104 40-41 0.9412771126628385 1104 42-43 1.0456769337325866 1104 44-45 0.8635552026866264 1104 46-47 0.7972440575791495 1104 48-49 0.6407067871075256 1104 50-51 0.3998591780536884 1104 52-53 0.6343017891692 1104 54-55 0.744047845308522 1104 56-57 0.6676463801018215 1104 58-59 0.5492808053187659 1104 60-61 0.5973706337894598 1104 62-63 0.8436976278716557 1104 64-65 0.9218765140415783 1104 66-67 0.8612560155810804 1104 68-69 0.8925092596572597 1104 70-71 1.085817717252553 1104 72-73 0.849996574352577 1104 74-75 0.5490822182283814 1104 76-77 0.7263427022124986 1104 78-79 1.0925427816098718 1104 80-81 1.021631747198935 1104 82-83 1.2632080812182274 1104 84-85 1.1364687092751922 1104 86-87 1.253957730661881 1104 88-89 1.3205144906365085 1104 90-91 1.5150302607123045 1104 92-93 1.1863605431902506 1104 94-95 1.5413365145107036 1104 96-97 1.8709933979387579 1104 98-99 2.1736357018417216 1104 100-101 2.1307482088778826 1105 1 0.5596022409452281 1105 2 0.3216629755782954 1105 3 0.32264403652564866 1105 4 0.3595098013306952 1105 5 0.2538817283305974 1105 6 0.4214117473820025 1105 7 0.5104712237239468 1105 8 0.4293360033132032 1105 9 0.5866511764246667 1105 10-11 0.6268524337418526 1105 12-13 0.5915675351070959 1105 14-15 0.7630072576402398 1105 16-17 0.7808801871679805 1105 18-19 0.6654211602243763 1105 20-21 0.7760490297777665 1105 22-23 0.7085077704172846 1105 24-25 0.730858670333042 1105 26-27 0.8022171504095255 1105 28-29 0.7584880107017469 1105 30-31 0.8449692415762939 1105 32-33 1.0269176181188868 1105 34-35 1.0015727031246655 1105 36-37 1.015497535896202 1105 38-39 1.211062966228333 1105 40-41 1.194260997310849 1105 42-43 1.1887914375493835 1105 44-45 1.3804271280471028 1105 46-47 1.3065502492670191 1105 48-49 1.2490867701439967 1105 50-51 1.3591501025659838 1105 52-53 1.3414298638087274 1105 54-55 1.3691810090065744 1105 56-57 1.3403283139440632 1105 58-59 1.4780441640973976 1105 60-61 1.2771263589803041 1105 62-63 1.2075381707045594 1105 64-65 1.2296797371119723 1105 66-67 1.267464666980569 1105 68-69 1.2300257990974615 1105 70-71 1.4808338326045423 1105 72-73 1.4830245641744604 1105 74-75 1.5148701741232067 1105 76-77 1.550973745469495 1105 78-79 1.6615605933147037 1105 80-81 1.6510057930004614 1105 82-83 1.669013000641467 1105 84-85 1.6978427550767208 1105 86-87 1.8808788502547564 1105 88-89 1.89493009708265 1105 90-91 1.967790226785251 1105 92-93 1.6258177102810052 1105 94-95 1.3408343940696525 1105 96-97 1.5442079865731948 1105 98-99 1.7677222158366312 1105 100-101 1.6982732300822931 1106 1 0.20590344649138004 1106 2 0.2426335165303115 1106 3 -0.04060117873017077 1106 4 0.20721777312439116 1106 5 0.2869733846168572 1106 6 0.28623513859717065 1106 7 0.5347524918009299 1106 8 0.3040571520066919 1106 9 0.27306012521823675 1106 10-11 0.1640476314308401 1106 12-13 0.24699817095550003 1106 14-15 0.5030596387447162 1106 16-17 0.1771808603858318 1106 18-19 0.04846543929215841 1106 20-21 0.2609795254463876 1106 22-23 0.23039214161335764 1106 24-25 0.21462017306033232 1106 26-27 0.14647350552218086 1106 28-29 0.21179891526947614 1106 30-31 -0.009204389718931338 1106 32-33 0.16828697943139304 1106 34-35 -0.3019537944035591 1106 36-37 -0.08777415382027698 1106 38-39 0.18997779724958974 1106 40-41 0.2386234631974773 1106 42-43 -0.1672900583641166 1106 44-45 0.03033716456481983 1106 46-47 -0.010441669989425861 1106 48-49 0.12946550398338985 1106 50-51 -0.3466884742010805 1106 52-53 -0.39281304114228277 1106 54-55 0.03131330323505921 1106 56-57 -0.8325637547191889 1106 58-59 -0.43559692596900135 1106 60-61 0.38733251198565455 1106 62-63 -0.046543190352430486 1106 64-65 0.002676142121536884 1106 66-67 -0.5203897305834957 1106 68-69 -0.4355204939457096 1106 70-71 -0.4264296095721818 1106 72-73 0.0179463651475551 1106 74-75 0.3392802656481848 1106 76-77 0.4908057426588037 1106 78-79 0.6109379466493863 1106 80-81 0.6930441135401679 1106 82-83 0.5273345340308744 1106 84-85 0.8288629445146185 1106 86-87 0.5876443880305438 1106 88-89 0.36313838649889973 1106 90-91 0.5313835847476369 1106 92-93 0.817127628266956 1106 94-95 0.7426624094432945 1106 96-97 0.7670324495426648 1106 98-99 1.120837933361944 1106 100-101 0.6614446291475247 1107 1 0.1627555814554391 1107 2 0.05296435549608702 1107 3 0.2496580477476762 1107 4 0.08535920108276684 1107 5 0.1380750636212582 1107 6 0.4095433941491464 1107 7 0.27029253578762535 1107 8 0.18715512969149017 1107 9 0.5555438984185344 1107 10-11 0.4789873508815461 1107 12-13 0.4094277654196148 1107 14-15 0.5108297277909557 1107 16-17 0.44408992325462293 1107 18-19 0.5436721633560992 1107 20-21 0.5984620916946426 1107 22-23 0.5809775133545898 1107 24-25 0.7191677001497041 1107 26-27 0.6849373908349108 1107 28-29 0.7625820930401019 1107 30-31 0.5048056413936166 1107 32-33 0.7424930731981618 1107 34-35 0.6372258805240207 1107 36-37 0.7006303896836386 1107 38-39 0.635863767102606 1107 40-41 0.5420377964235215 1107 42-43 0.6692752243308746 1107 44-45 0.6609675958490158 1107 46-47 0.7870656387757293 1107 48-49 0.8068606332613726 1107 50-51 0.7027288361733426 1107 52-53 0.710996233613649 1107 54-55 0.7616899393256134 1107 56-57 0.655416679247125 1107 58-59 0.49287909591706125 1107 60-61 0.5049432833621168 1107 62-63 0.6759486962477226 1107 64-65 0.7220634798535457 1107 66-67 0.6657752463503108 1107 68-69 0.6891043451273475 1107 70-71 0.8361820334918697 1107 72-73 0.9418074717884757 1107 74-75 0.7646815772027402 1107 76-77 1.0347401381099353 1107 78-79 1.3225887217808108 1107 80-81 1.3312076018997878 1107 82-83 1.1515050897652337 1107 84-85 1.1272432819247662 1107 86-87 1.1445934144225625 1107 88-89 1.1931629949100149 1107 90-91 1.0399351752422206 1107 92-93 1.160671440626146 1107 94-95 1.0442713435705322 1107 96-97 1.4324036543490166 1107 98-99 1.5300748044058246 1107 100-101 1.5570120977667727 1108 1 0.1809971458085684 1108 2 0.14492386856716521 1108 3 0.1622295628232635 1108 4 0.03181946584515316 1108 5 0.20933174532175514 1108 6 0.17699672664726762 1108 7 0.11673087622026657 1108 8 0.22363042827997504 1108 9 0.19219250361355478 1108 10-11 0.28831745820635746 1108 12-13 0.1748452672988421 1108 14-15 0.3558038362015452 1108 16-17 0.35149225558570407 1108 18-19 0.4448469420247747 1108 20-21 0.27259347063058215 1108 22-23 0.25647394282088243 1108 24-25 0.5128545371997362 1108 26-27 0.614739080052324 1108 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7 1460.0 8 1773.0 9 2203.0 10 3013.0 11 4028.0 12 5742.0 13 7329.0 14 9138.0 15 11023.0 16 13526.0 17 16030.0 18 14020.0 19 11363.0 20 11596.0 21 12625.0 22 13628.0 23 15319.0 24 17284.0 25 19717.0 26 22643.0 27 26367.0 28 30629.0 29 36047.0 30 42015.0 31 49548.0 32 58996.0 33 72012.0 34 89794.0 35 114873.0 36 146093.0 37 178489.0 38 104364.0 39 5735.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.955487776425024 21.761738916486024 15.413285369464605 21.869487937624346 2 14.555639728638061 22.31967238175475 41.49743347589913 21.62725441370806 3 17.706117970008087 27.00571776073057 31.793535499038544 23.4946287702228 4 11.447616976747346 19.07369908810459 40.215555902858 29.263128032290066 5 11.61879141253384 39.42804606764677 36.06593036831345 12.887232151505934 6 27.829270218838392 39.8555739635725 19.064440608104263 13.250715209484836 7 25.33979157243923 34.982684779832375 22.81086145023561 16.866662197492793 8 23.96306246828386 36.359725123936926 21.56952263280896 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29.626660476241202 32.047462003363705 19.27610217087866 34-35 19.395960250199806 29.798937792572865 31.891604418776105 18.91349753845122 36-37 19.396168664329057 29.347939568484556 31.66538921525438 19.59050255193201 38-39 19.030260341195266 29.878114239524084 31.764171739052028 19.327453680228622 40-41 19.408002355905527 29.465580313591733 32.03331365835381 19.093103672148928 42-43 19.186139023056224 29.439767269468504 31.704782542833946 19.66931116464132 44-45 19.39144964996074 29.4571305307285 31.537088720306688 19.614331099004076 46-47 19.558306892582884 29.452556617993626 31.01978357826823 19.969352911155262 48-49 19.317124159937826 29.46795143187065 31.1540148629811 20.060909545210425 50-51 19.293130473699726 29.65691608390125 30.848833090470222 20.2011203519288 52-53 19.3034695016025 29.943302697417124 30.864077072696844 19.889150728283532 54-55 19.559256932268397 29.547713580640767 30.7311934790457 20.161836008045135 56-57 19.59295207113093 29.7201073514351 30.40598731162377 20.280953265810197 58-59 19.587806883490128 29.44087446680147 30.627806001013152 20.34351264869525 60-61 19.608916337524878 29.421902704711766 30.689719108298362 20.279461849464994 62-63 19.462898479896555 29.091662678898082 30.917169804276924 20.528269036928435 64-65 19.44819289068343 29.160983899616213 30.693178091803535 20.697645117896823 66-67 19.46303856183558 29.294378521881853 30.312014922618825 20.93056799366374 68-69 19.604855227276968 28.98945873345291 30.571369906482744 20.83431613278738 70-71 19.651948697777772 28.98163128575059 30.672583216330423 20.69383680014121 72-73 19.833174858359016 28.591388720230782 30.591270765631425 20.984165655778778 74-75 19.693855556441882 28.99847929137694 30.188272553076295 21.119392599104884 76-77 19.570530178123352 28.91727544501138 30.414025845748622 21.09816853111664 78-79 19.65404329528067 28.790797451541923 30.143928747019427 21.41123050615798 80-81 19.803466172892925 28.76982889721743 29.9641759514371 21.462528978452543 82-83 19.98235295365317 28.784453630900998 30.05644085376071 21.176752561685124 84-85 19.99938066258922 28.486424208796457 30.00184952815822 21.512345600456104 86-87 19.899997709093963 28.604380042644788 29.987535774194722 21.50808647406652 88-89 20.442222220336266 28.393344982894835 29.84263756482862 21.32179523194028 90-91 20.429970191053073 28.342686199836635 29.930431646281306 21.296911962828986 92-93 20.62853447749136 28.323130163816458 29.840791519290693 21.207543839401488 94-95 20.629628073588307 28.178960595516816 29.73624675194056 21.455164578954314 96-97 20.96744283155407 27.84255130806225 29.49381307285675 21.69619278752693 98-99 21.097198049267288 27.724580776610214 29.22823521824395 21.949985955878546 100-101 21.5570486550643 27.439269252875757 29.61804556217489 21.385636529885055 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2410.0 1 1485.0 2 677.0 3 779.0 4 664.5 5 690.5 6 1208.5 7 2299.0 8 3199.5 9 3215.0 10 2425.0 11 1726.0 12 1641.0 13 1627.0 14 1583.5 15 1566.0 16 1623.5 17 1773.5 18 1945.5 19 2145.5 20 2439.5 21 2804.0 22 3300.0 23 3970.5 24 4772.0 25 6078.5 26 8394.0 27 11186.5 28 13174.5 29 15578.5 30 19557.0 31 23707.0 32 28274.0 33 33625.0 34 40112.5 35 45465.0 36 49609.0 37 53593.0 38 53430.5 39 51706.5 40 51088.0 41 49742.5 42 48300.5 43 47768.0 44 47511.0 45 46510.0 46 44659.0 47 42905.0 48 42546.0 49 40604.0 50 36173.0 51 31427.5 52 27056.0 53 23273.5 54 19802.0 55 16753.0 56 13827.5 57 11192.0 58 8792.0 59 6626.0 60 4881.0 61 3555.0 62 2531.5 63 1704.5 64 1137.5 65 792.0 66 631.0 67 526.5 68 407.5 69 314.0 70 252.5 71 220.5 72 199.5 73 183.5 74 169.5 75 144.0 76 95.0 77 58.5 78 39.0 79 23.0 80 15.0 81 12.5 82 12.0 83 8.5 84 7.0 85 5.5 86 1.5 87 1.0 88 2.0 89 1.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007041369317275618 2 0.10858979188087699 3 0.11571599697305955 4 0.12046680036784793 5 0.15864289907596874 6 0.09510090367067431 7 0.025874911346615223 8 0.02765646261966086 9 0.022311808800523945 10-11 0.05064695761944029 12-13 0.05425247805298503 14-15 0.027189865857672718 16-17 0.030752968403763993 18-19 0.0354613539110989 20-21 0.058451848910878325 22-23 0.030201535866868915 24-25 0.04763528761024409 26-27 0.055991611438577206 28-29 0.05293752354192753 30-31 0.06765653048828078 32-33 0.049586510433103595 34-35 0.06188769779460919 36-37 0.052683016217206724 38-39 0.033128370101158176 40-41 0.03669147264724946 42-43 0.03304353432625125 44-45 0.008016980728705372 46-47 0.014379663846725508 48-49 0.009756114114297542 50-51 0.008525995378146982 52-53 0.010222710876285686 54-55 0.012513276798772933 56-57 0.016033961457410744 58-59 0.020954436402012982 60-61 0.02583249345916176 62-63 0.012216351586598662 64-65 0.009459188902123269 66-67 0.01141041172498278 68-69 0.012046680036784793 70-71 0.030413625304136254 72-73 0.0278685520569282 74-75 0.0296925212174273 76-77 0.025026553597545867 78-79 0.0063202652305666684 80-81 0.005980922130938928 82-83 0.00682927988000828 84-85 0.005853668468578526 86-87 0.01484626060871365 88-89 0.03809126293321388 90-91 0.03482508559929688 92-93 0.030201535866868915 94-95 0.04619307943682619 96-97 0.04220579801620024 98-99 0.027062612195312315 100-101 0.07507966079263761 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1178748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.98915844814967 #Duplication Level Percentage of deduplicated Percentage of total 1 77.68455570732328 44.271774541787565 2 13.083302301119943 14.912127757271312 3 4.323238226519215 7.3913312490060346 4 1.8591778533649974 4.238119250748345 5 0.8998768310510722 2.564161165429418 6 0.5376872669278644 1.8385406910302766 7 0.3223329741787718 1.2858639456976153 8 0.2066697971519187 0.9422350253070116 9 0.1358273309281382 0.6966616755467635 >10 0.7882831536220085 8.702247406426448 >50 0.09465720177077402 3.7763032240540206 >100 0.06141716261099586 6.297402878531018 >500 0.0019332257301105382 0.7484977835325382 >1k 7.435483577348224E-4 1.0813894608101435 >5k 2.9741934309392897E-4 1.2533439448214116 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8264 0.7010828438309121 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6525 0.5535534312677519 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4879 0.41391374577093665 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3938 0.33408328158351064 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 0.12063647191766179 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1343 0.11393444570001392 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.483577490693515E-5 0.0 10-11 0.0 0.0 0.0 1.696715498138703E-4 0.0 12-13 0.0 0.0 0.0 2.96925212174273E-4 0.0 14-15 0.0 0.0 0.0 4.241788745346758E-4 0.0 16-17 0.0 0.0 0.0 6.786861992554812E-4 0.0 18-19 0.0 0.0 0.0 8.05939861615884E-4 0.0 20-21 0.0 0.0 0.0 0.001187700848697092 0.0 22-23 0.0 0.0 0.0 0.002417819584847652 0.0 24-25 0.0 0.0 0.0 0.00576883269367159 0.0 26-27 0.0 0.0 0.0 0.011792172712063988 0.0 28-29 0.0 0.0 0.0 0.02468721049791813 0.0 30-31 0.0 0.0 0.0 0.05883360989795953 0.0 32-33 0.0 0.0 0.0 0.12000020360585978 0.0 34-35 0.0 0.0 0.0 0.2159918829130569 0.0 36-37 0.0 0.0 0.0 0.36135798321609025 0.0 38-39 0.0 0.0 0.0 0.5562257581773203 0.0 40-41 0.0 0.0 0.0 0.7633098847251492 0.0 42-43 0.0 0.0 0.0 0.9706485185976986 0.0 44-45 0.0 0.0 0.0 1.2268101409291892 0.0 46-47 0.0 0.0 0.0 1.5190693854835808 0.0 48-49 0.0 0.0 0.0 1.8214240872518976 0.0 50-51 0.0 0.0 0.0 2.115337629416975 0.0 52-53 0.0 0.0 0.0 2.4090815000322374 0.0 54-55 0.0 0.0 0.0 2.7412135587928885 0.0 56-57 0.0 0.0 0.0 3.1362937625344856 0.0 58-59 0.0 0.0 0.0 3.5490622253441786 0.0 60-61 0.0 0.0 0.0 3.9718837274803436 0.0 62-63 0.0 0.0 0.0 4.3879183676239535 0.0 64-65 0.0 0.0 0.0 4.841620091826243 0.0 66-67 0.0 0.0 0.0 5.3494046225317025 0.0 68-69 0.0 0.0 0.0 5.897231638993237 0.0 70-71 0.0 8.483577490693515E-5 0.0 6.484973887548484 0.0 72-73 0.0 8.483577490693515E-5 0.0 7.0582516364820975 0.0 74-75 0.0 8.483577490693515E-5 0.0 7.662918622131278 0.0 76-77 0.0 8.483577490693515E-5 0.0 8.324171069643384 0.0 78-79 0.0 8.483577490693515E-5 0.0 9.037852026047975 0.0 80-81 0.0 8.483577490693515E-5 0.0 9.78041956380838 0.0 82-83 0.0 8.483577490693515E-5 0.0 10.510007228008021 0.0 84-85 0.0 8.483577490693515E-5 0.0 11.252829273093145 0.0 86-87 0.0 8.483577490693515E-5 0.0 12.055206032162939 0.0 88-89 0.0 8.483577490693515E-5 0.0 12.87726469101114 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4810 0.0 33.511444 1 TATCGCG 60 0.003943507 31.678936 7 GTATCAA 7110 0.0 30.562233 1 TCAACGC 8610 0.0 25.128422 4 CAACGCA 8660 0.0 24.92843 5 ATCAACG 8725 0.0 24.797215 3 TATCAAC 8730 0.0 24.783012 2 AACGCAG 8845 0.0 24.496859 6 CATTCCG 275 1.8189894E-12 24.191187 9 GTACATG 5265 0.0 23.932386 1 TAGGACG 265 3.092282E-11 23.32676 4 TACATGG 5390 0.0 22.84904 2 GTATAGC 755 0.0 22.67222 1 ACATGGG 5260 0.0 22.328945 3 ACGCAGA 9955 0.0 21.670874 7 CGCAGAG 9945 0.0 21.644884 8 AGTACTT 5785 0.0 21.437819 12-13 GAGTACT 5765 0.0 21.347345 12-13 GTGTAGC 830 0.0 21.196402 1 CATGGGG 2045 0.0 20.461916 4 >>END_MODULE