FastQCFastQC Report
Thu 26 May 2016
SRR938415_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938415_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178748
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79530.6746989178348552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64170.5443911675778029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50120.425196903833559No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40950.34740249824389946No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT18290.1551646323047844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.12428441023865999No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.1187700848697092No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT11800.10010621439018347RNA PCR Primer, Index 39 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGC459.644086E-442.2053878
GGTATCA47050.034.9496151
GTATCAA68800.028.6672171
CATTCCG2500.026.5893949
AGTCGAA1109.50256E-525.8987586
GTACATG54050.025.8510111
TACATGG54400.024.7226492
GCGTAAA1000.001648529723.7627071
TAGTCGA1201.70908E-423.753635
GTGTACG1201.7154844E-423.7405329
ACATGGG54100.023.6219083
CATGGGG21950.023.5913054
TCAACGC83500.023.2700234
ATCAACG84100.023.0475163
TATCAAC84550.023.0450522
CAACGCA84750.022.9268055
AACGCAG87150.022.2831366
GAGTACT56850.022.00749212-13
AGTACTT58400.020.65100912-13
GTACTTT62200.020.07639714-15
Produced by FastQC (version 0.11.5)