##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938415_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1178748 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.803112285238235 31.0 31.0 34.0 30.0 34.0 2 32.025667063698094 31.0 31.0 34.0 30.0 34.0 3 32.08100713638539 33.0 31.0 34.0 30.0 34.0 4 35.75321782094222 37.0 35.0 37.0 33.0 37.0 5 35.66238670182261 37.0 35.0 37.0 33.0 37.0 6 35.65975679280049 37.0 35.0 37.0 33.0 37.0 7 35.6049647592191 37.0 35.0 37.0 33.0 37.0 8 35.59638701401826 37.0 35.0 37.0 33.0 37.0 9 37.16527196652719 39.0 37.0 39.0 33.0 39.0 10-11 37.08912082989748 39.0 37.0 39.0 33.0 39.0 12-13 37.002505200433006 39.0 37.0 39.0 33.0 39.0 14-15 38.03174639532792 40.0 37.0 41.0 33.0 41.0 16-17 37.958028772901415 40.0 37.0 41.0 33.0 41.0 18-19 38.22177895529833 40.0 37.5 41.0 33.0 41.0 20-21 38.22806443786119 40.0 37.5 41.0 33.0 41.0 22-23 38.10885660039296 40.0 37.0 41.0 33.0 41.0 24-25 37.99910540675361 40.0 37.0 41.0 33.0 41.0 26-27 37.8193252501807 40.0 37.0 41.0 32.5 41.0 28-29 37.704944144125804 40.0 37.0 41.0 32.5 41.0 30-31 37.445895560374225 39.0 36.0 41.0 32.0 41.0 32-33 37.220322325043185 39.0 36.0 41.0 31.5 41.0 34-35 37.06421474310031 39.0 36.0 41.0 31.0 41.0 36-37 36.994872101585756 39.0 35.5 41.0 31.0 41.0 38-39 36.829242128088445 39.0 35.0 41.0 31.0 41.0 40-41 36.67131100116394 39.0 35.0 41.0 30.0 41.0 42-43 36.45361604006963 39.0 35.0 41.0 30.0 41.0 44-45 36.12410795182685 39.0 35.0 40.5 30.0 41.0 46-47 35.70392399393255 39.0 35.0 40.0 28.0 41.0 48-49 35.35942287919046 38.5 35.0 40.0 27.0 41.0 50-51 35.032677892136405 38.0 34.5 40.0 26.0 41.0 52-53 34.62495588539704 38.0 34.0 40.0 24.0 41.0 54-55 34.347763050287256 38.0 33.5 40.0 23.5 41.0 56-57 34.05198100017985 38.0 33.0 40.0 23.0 41.0 58-59 33.6440757481667 38.0 33.0 40.0 22.0 41.0 60-61 33.30919840373006 37.0 32.5 40.0 20.5 41.0 62-63 32.87665853939943 37.0 32.0 39.5 18.5 41.0 64-65 32.41277100788294 36.0 31.0 39.0 15.0 40.0 66-67 31.94932674329034 36.0 31.0 39.0 12.5 40.0 68-69 31.496107734647268 35.0 30.0 38.5 8.0 40.0 70-71 31.00487975377265 35.0 29.5 38.0 7.5 40.0 72-73 30.54242170506334 34.5 29.0 37.5 6.5 39.5 74-75 29.978324035332406 34.0 29.0 37.0 2.0 39.0 76-77 28.645975221166864 32.5 27.0 35.5 2.0 38.0 78-79 29.345257849854253 34.0 29.0 36.0 2.0 38.0 80-81 28.960391025053703 34.0 28.5 35.5 2.0 37.5 82-83 28.540684268393242 34.0 28.0 35.0 2.0 37.0 84-85 28.01178708256557 33.0 26.5 35.0 2.0 36.5 86-87 27.44477530396658 33.0 26.0 35.0 2.0 36.0 88-89 26.756699481144402 32.0 25.5 35.0 2.0 35.5 90-91 26.20736493296277 32.0 24.5 34.0 2.0 35.0 92-93 25.47282073861419 31.5 21.5 34.0 2.0 35.0 94-95 24.744491613135292 31.0 19.0 34.0 2.0 35.0 96-97 23.771242029678945 31.0 4.5 34.0 2.0 35.0 98-99 22.527451160044386 31.0 2.0 34.0 2.0 35.0 100-101 18.546607502197247 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07861348953315073 1101 2 0.12414164408612294 1101 3 0.09931621138023417 1101 4 0.12960130358139565 1101 5 -0.02872127328302554 1101 6 0.05686108143984825 1101 7 0.05964543366580699 1101 8 0.20639141617364487 1101 9 0.07241400383745145 1101 10-11 0.031131832145227634 1101 12-13 0.003465930107388715 1101 14-15 0.1535507463357746 1101 16-17 -0.024845250265158825 1101 18-19 0.1416185707013895 1101 20-21 0.24544461575798238 1101 22-23 0.23261845283472127 1101 24-25 0.31646061442928186 1101 26-27 0.20844408359835143 1101 28-29 0.039866848231135066 1101 30-31 0.0805648079947261 1101 32-33 0.1455872279893029 1101 34-35 0.10977899874500707 1101 36-37 0.11683732186801166 1101 38-39 0.25736512554630053 1101 40-41 0.19929589861999375 1101 42-43 0.15646531470094516 1101 44-45 0.24521402124787528 1101 46-47 0.06749516170923897 1101 48-49 0.13563862680989303 1101 50-51 0.22382852153155142 1101 52-53 0.20017441206469755 1101 54-55 0.3343902243228669 1101 56-57 0.14395642290076438 1101 58-59 0.10905664207918875 1101 60-61 0.3242547573710013 1101 62-63 0.501386182624266 1101 64-65 0.5124261847404625 1101 66-67 0.3596093551212185 1101 68-69 0.4112459356399931 1101 70-71 0.37752550279132535 1101 72-73 0.5190832974685549 1101 74-75 0.369884321930396 1101 76-77 0.23050738645229174 1101 78-79 0.3750798026481199 1101 80-81 0.5025793354575576 1101 82-83 0.45904917838346293 1101 84-85 0.4077627968209008 1101 86-87 0.49793041535109595 1101 88-89 0.3697008101557948 1101 90-91 0.4021213806560624 1101 92-93 0.6223288031956358 1101 94-95 0.7081885001648125 1101 96-97 0.774861837831974 1101 98-99 0.8104484875459903 1101 100-101 1.120720455746941 1102 1 0.2442063644808421 1102 2 0.22071011991443612 1102 3 0.19529881722801434 1102 4 0.25376844027832846 1102 5 0.09013209546936451 1102 6 0.19592696452058078 1102 7 0.18675920524929523 1102 8 0.22464344943359293 1102 9 0.19758094039246288 1102 10-11 0.42603230702741257 1102 12-13 0.3790536195599117 1102 14-15 0.5059069625618307 1102 16-17 0.4481501464707023 1102 18-19 0.529799735527348 1102 20-21 0.49607324333025815 1102 22-23 0.4390693907725307 1102 24-25 0.5326375398855205 1102 26-27 0.41772202613941545 1102 28-29 0.31416453204329997 1102 30-31 0.3393082806389671 1102 32-33 0.4711371633980477 1102 34-35 0.3750593793785484 1102 36-37 0.4256690389037274 1102 38-39 0.4971283212669064 1102 40-41 0.5475187293545076 1102 42-43 0.28953057916120173 1102 44-45 0.12644525794299 1102 46-47 0.2579256487818853 1102 48-49 0.03738941373152471 1102 50-51 0.042759672711483176 1102 52-53 0.034526952957321555 1102 54-55 0.0920806775533336 1102 56-57 0.23425772746218598 1102 58-59 0.24537694192817128 1102 60-61 0.12384082749344572 1102 62-63 0.13229309843729453 1102 64-65 0.2292467484128835 1102 66-67 0.23313376333795688 1102 68-69 0.3702568706666476 1102 70-71 0.22152397443485583 1102 72-73 0.2153542896266316 1102 74-75 0.26699545527949553 1102 76-77 0.27147689210175585 1102 78-79 0.5520384642445251 1102 80-81 0.36449642211900724 1102 82-83 0.4415754242669081 1102 84-85 0.4983993390963306 1102 86-87 0.40459514124763984 1102 88-89 0.6890991107689608 1102 90-91 0.5016646931867648 1102 92-93 0.8186643866093313 1102 94-95 0.8137851960036819 1102 96-97 0.7041735315784514 1102 98-99 1.00810637222785 1102 100-101 1.6660848959863834 1103 1 0.2763287925522171 1103 2 0.26101576321674713 1103 3 0.2535332575934248 1103 4 0.23886619490993155 1103 5 0.17001769266905598 1103 6 0.21790254720648505 1103 7 0.17085380099760528 1103 8 0.25241174107535613 1103 9 0.34776449712429525 1103 10-11 0.29530362948963074 1103 12-13 0.2771177363532189 1103 14-15 0.5170697731142084 1103 16-17 0.3222729160429765 1103 18-19 0.20160670214563936 1103 20-21 0.2667041662364298 1103 22-23 0.3044454686347322 1103 24-25 0.3556120126935056 1103 26-27 0.5491977368881606 1103 28-29 0.41182738528328855 1103 30-31 0.3120906591927053 1103 32-33 0.43302139459414946 1103 34-35 0.43672803813377215 1103 36-37 0.48536339560141784 1103 38-39 0.2845589538973101 1103 40-41 0.36193220156487627 1103 42-43 0.4200436842581041 1103 44-45 0.49062608266765295 1103 46-47 0.34834227987554556 1103 48-49 0.5854791430920656 1103 50-51 0.81564663591476 1103 52-53 0.5599474259360733 1103 54-55 0.4286191390918006 1103 56-57 0.41666616253432665 1103 58-59 0.46854777775161693 1103 60-61 0.4462560925835177 1103 62-63 0.5997245848199455 1103 64-65 0.6064622354785385 1103 66-67 0.7723828882419035 1103 68-69 0.6746536947083115 1103 70-71 0.788637438107699 1103 72-73 0.5568253047380445 1103 74-75 0.556910506931505 1103 76-77 0.3799695925200943 1103 78-79 0.7333989184407166 1103 80-81 0.8585692471851765 1103 82-83 1.0028812383196701 1103 84-85 1.126455772119467 1103 86-87 1.022045837055714 1103 88-89 0.9668375210822866 1103 90-91 0.8900451251853987 1103 92-93 1.0575784137586517 1103 94-95 0.8383049603680632 1103 96-97 1.0687940387072814 1103 98-99 1.3660526712118894 1103 100-101 1.6939791160837139 1104 1 0.21965709823345847 1104 2 0.26554869433791595 1104 3 0.2590556306452072 1104 4 0.07361330400998867 1104 5 0.05443398226181273 1104 6 0.08488293936334657 1104 7 0.18730427862455912 1104 8 0.18917594419249895 1104 9 0.24097716680755354 1104 10-11 0.1688058919330757 1104 12-13 0.30529470970666495 1104 14-15 0.4271270883479943 1104 16-17 0.4669425993008929 1104 18-19 0.3637263602653036 1104 20-21 0.26912146135961024 1104 22-23 0.4181930804999965 1104 24-25 0.3434551499484044 1104 26-27 0.3101811456362782 1104 28-29 0.33899681715859487 1104 30-31 0.3380755762364487 1104 32-33 0.4062943961024459 1104 34-35 0.6631453332569563 1104 36-37 0.4461637978135755 1104 38-39 0.31159163363587794 1104 40-41 0.4592273247423577 1104 42-43 0.5690190487628826 1104 44-45 0.34732995395975763 1104 46-47 0.22748959008167446 1104 48-49 0.16892610136255115 1104 50-51 0.33253954692531806 1104 52-53 0.11429634499086916 1104 54-55 0.1328584553311103 1104 56-57 0.24628707756700408 1104 58-59 0.3703496882594024 1104 60-61 0.22670455914963128 1104 62-63 0.2694440418335162 1104 64-65 0.31864926413615535 1104 66-67 0.39469560820168326 1104 68-69 0.3244455499119283 1104 70-71 0.43753236017909103 1104 72-73 0.18436477180692634 1104 74-75 0.36646505695664544 1104 76-77 0.11335320237428803 1104 78-79 0.4023340617288014 1104 80-81 0.1873957931881911 1104 82-83 0.30341819156250693 1104 84-85 0.2108961944422454 1104 86-87 0.38466222720151677 1104 88-89 0.6762311862406563 1104 90-91 0.6310064122643304 1104 92-93 0.7937754977204428 1104 94-95 0.7074371876179377 1104 96-97 0.590965984408637 1104 98-99 1.191454380613596 1104 100-101 1.473709633931879 1105 1 0.2521592186745103 1105 2 0.33240535252281944 1105 3 0.0846502023161122 1105 4 0.22388302411176397 1105 5 0.0885781722533352 1105 6 0.25412466964324665 1105 7 0.13749935920131406 1105 8 0.1502751808337237 1105 9 0.335464020073033 1105 10-11 0.23008154926979785 1105 12-13 0.29114373430377327 1105 14-15 0.41792946324196834 1105 16-17 0.3101758478165806 1105 18-19 0.4616771660328993 1105 20-21 0.3782071271781007 1105 22-23 0.31710402197581544 1105 24-25 0.32969772840472444 1105 26-27 0.14346698109005018 1105 28-29 0.17390775863442798 1105 30-31 0.2281315558802106 1105 32-33 0.46792289483018124 1105 34-35 0.49309953173023047 1105 36-37 0.6508694805955955 1105 38-39 0.5308893435595436 1105 40-41 0.5396174858958744 1105 42-43 0.7131818986356535 1105 44-45 0.6804732957748598 1105 46-47 0.913610031133409 1105 48-49 0.9389549393608618 1105 50-51 0.8954742373409275 1105 52-53 0.8886814170858628 1105 54-55 0.9372248675448489 1105 56-57 0.8785381377875368 1105 58-59 0.9937390012365057 1105 60-61 0.869086238538948 1105 62-63 0.9543532869565112 1105 64-65 0.9991072794033258 1105 66-67 0.798444547981159 1105 68-69 0.8863658213283827 1105 70-71 0.8752999598398219 1105 72-73 0.9181764766415306 1105 74-75 1.0070553877454493 1105 76-77 0.8119961201011776 1105 78-79 1.124529142305562 1105 80-81 1.2086035964112618 1105 82-83 1.118390201740624 1105 84-85 1.2412846592429219 1105 86-87 1.2367538302549548 1105 88-89 1.299535681575687 1105 90-91 1.27800217138223 1105 92-93 1.5805812653201023 1105 94-95 1.6201225141658604 1105 96-97 1.4280312939930333 1105 98-99 1.5827486978315761 1105 100-101 1.9135535694704693 1106 1 -0.006433092375559113 1106 2 0.02373372455641487 1106 3 0.09910398025004241 1106 4 0.1346044709091032 1106 5 0.017178752136295827 1106 6 -0.045203532045299255 1106 7 0.04556555245068239 1106 8 -0.004601833585276438 1106 9 -0.3824537955355396 1106 10-11 -0.09142841769035925 1106 12-13 0.016361190413306304 1106 14-15 -0.0903233997970645 1106 16-17 0.07845724365235185 1106 18-19 -0.01786545749388324 1106 20-21 0.20999919264738054 1106 22-23 0.22837625111831272 1106 24-25 0.30724873432776434 1106 26-27 0.3263764036558001 1106 28-29 0.3030786395667846 1106 30-31 0.27133638516253455 1106 32-33 0.3352762650006369 1106 34-35 0.27583896412631503 1106 36-37 0.3054906225462659 1106 38-39 0.21874551555126942 1106 40-41 0.32976195979581746 1106 42-43 0.2390292765638975 1106 44-45 0.11118118594488635 1106 46-47 -0.5590357469708565 1106 48-49 -0.06853740398379671 1106 50-51 0.22528710620007075 1106 52-53 0.23349671691090634 1106 54-55 0.4223108030763498 1106 56-57 0.2862945159677466 1106 58-59 0.3540056622249992 1106 60-61 0.2872773160069073 1106 62-63 0.08755282844439449 1106 64-65 0.30662694893392484 1106 66-67 0.14159472489160052 1106 68-69 -0.05645821577844501 1106 70-71 0.10142450336830322 1106 72-73 0.3078031725507664 1106 74-75 0.3804418119543236 1106 76-77 0.6368771447266859 1106 78-79 0.8220885945042546 1106 80-81 0.16186162303477403 1106 82-83 0.367708289191512 1106 84-85 0.45614584576720674 1106 86-87 0.6500755233428492 1106 88-89 0.890524073269944 1106 90-91 1.0158748455511777 1106 92-93 0.9843547631783665 1106 94-95 1.0261559230898136 1106 96-97 1.3286228881743298 1106 98-99 1.278476230915782 1106 100-101 1.6367705358379645 1107 1 0.16691137173773285 1107 2 0.21198672852595024 1107 3 0.0760257161152893 1107 4 0.11438467153135434 1107 5 0.09647885405668433 1107 6 0.13923550346591185 1107 7 0.10372521879549623 1107 8 0.13257765359421114 1107 9 0.20636178219216816 1107 10-11 0.295831532958708 1107 12-13 0.3015756926126443 1107 14-15 0.2602777293736338 1107 16-17 0.3037727275060149 1107 18-19 0.12500413804308153 1107 20-21 0.31557765794079984 1107 22-23 0.2751845334914478 1107 24-25 0.2335673294355871 1107 26-27 0.38904973537986365 1107 28-29 0.37263997955176364 1107 30-31 0.2722999352108104 1107 32-33 0.18908285764090493 1107 34-35 0.2816752477868718 1107 36-37 0.17790204567682366 1107 38-39 0.10867283021707408 1107 40-41 0.15168993157995914 1107 42-43 0.15046883508766484 1107 44-45 0.17989833002813782 1107 46-47 0.2482951456372291 1107 48-49 0.38938657144802136 1107 50-51 0.4999273674381399 1107 52-53 0.6080196355891587 1107 54-55 0.43510952370718314 1107 56-57 0.4021535305584578 1107 58-59 0.34466485919957535 1107 60-61 0.3046554138504902 1107 62-63 0.5405145546540311 1107 64-65 0.5483127256746201 1107 66-67 0.7580022321333111 1107 68-69 0.7011667037580835 1107 70-71 0.8597300097517397 1107 72-73 0.8012728914650467 1107 74-75 0.7661488176404063 1107 76-77 0.9158905955366841 1107 78-79 1.195055215574957 1107 80-81 1.1166009213933172 1107 82-83 1.2164983197676307 1107 84-85 1.2747070918781382 1107 86-87 1.4100030391673286 1107 88-89 1.5179074682919413 1107 90-91 1.3065373951703165 1107 92-93 1.5495137455836883 1107 94-95 1.873226717532468 1107 96-97 2.037119830562485 1107 98-99 2.29890523531445 1107 100-101 2.118724591196834 1108 1 0.07616813695948466 1108 2 0.11094427884750502 1108 3 0.2187270023426393 1108 4 0.13915655410046668 1108 5 0.010616392685271592 1108 6 0.1824551434892996 1108 7 0.1290921903147506 1108 8 0.05989328042123532 1108 9 0.21394133618866107 1108 10-11 0.23257462157476283 1108 12-13 0.15889659171897108 1108 14-15 0.36699570941204485 1108 16-17 0.3187373875745081 1108 18-19 0.2952674388031227 1108 20-21 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-0.7685831652559969 2316 62-63 -0.913490455983073 2316 64-65 -0.8439708232000882 2316 66-67 -0.855823557251977 2316 68-69 -0.984634508312233 2316 70-71 -0.6697932099325037 2316 72-73 -0.9796691922881742 2316 74-75 -1.0264975825387452 2316 76-77 -1.0341237505611751 2316 78-79 -1.0919104797559136 2316 80-81 -1.1296900874522748 2316 82-83 -1.009527951086696 2316 84-85 -1.306521563927479 2316 86-87 -1.5056366568353567 2316 88-89 -1.5679784207462433 2316 90-91 -1.7946743252417328 2316 92-93 -1.9351201839874648 2316 94-95 -1.7311586396503778 2316 96-97 -1.9142014100600662 2316 98-99 -1.8184563423364288 2316 100-101 -2.094468920168076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 24.0 5 48.0 6 124.0 7 311.0 8 584.0 9 1064.0 10 1821.0 11 2801.0 12 4000.0 13 5560.0 14 7112.0 15 9479.0 16 11665.0 17 14758.0 18 13349.0 19 10338.0 20 9540.0 21 10201.0 22 11120.0 23 12163.0 24 14058.0 25 16346.0 26 18522.0 27 22466.0 28 26938.0 29 32567.0 30 38699.0 31 46103.0 32 55843.0 33 68683.0 34 87483.0 35 116414.0 36 158566.0 37 215296.0 38 129447.0 39 5244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.80507983531554 22.434313278851384 15.751154411621233 22.009452474211848 2 14.616870627738365 22.944729350532945 40.68382553901572 21.75457448271296 3 17.506031823595883 27.20114901573534 32.192886011259404 23.099933149409374 4 11.561598884238942 19.83037920775196 39.62376998626508 28.98425192174402 5 11.483219881512163 39.741805157106725 36.14965455193604 12.625320409445075 6 27.772348288183736 40.23718385948481 18.623064471795498 13.367403380535958 7 25.21751892686138 35.19335769816789 22.531957636407444 17.05716573856329 8 23.95668964019451 36.82593735047695 21.298954483910045 17.918418525418495 9 24.96258742326604 17.51027361234123 22.445509981777274 35.081628982615456 10-11 22.518765673409412 28.662317984844936 29.569000329162808 19.249916012582844 12-13 23.797113547594567 27.024817857591277 29.128405732183637 20.04966286263052 14-15 20.933906144485505 28.83444977213111 27.88556162979704 22.346082453586348 16-17 19.06824020061964 31.724125937011134 29.121618870191085 20.086014992178143 18-19 18.792566350059555 30.126286534526464 32.064402230162855 19.01674488525113 20-21 20.003342529531334 29.244121729156696 31.843405036530285 18.909130704781685 22-23 19.478081829195045 29.714196757916028 31.416596252973495 19.391125159915433 24-25 19.131327091560284 29.83396985504443 32.06190189548148 18.972801157913803 26-27 19.196243747497302 29.830843078301356 31.942441343549998 19.030471830651347 28-29 18.642449446361734 29.83915985435394 32.15738223946085 19.361008459823474 30-31 19.292907569469982 29.58726288538879 32.16173190009404 18.958097645047182 32-33 19.01151522786764 29.70688703691685 32.02295495160737 19.25864278360814 34-35 19.252894530978224 30.027481711383487 31.855325207321894 18.86429855031639 36-37 19.337536487679046 29.50168861584414 31.594384291629897 19.566390604846923 38-39 18.912041171008408 30.006443575026076 31.729749930749595 19.351765323215915 40-41 19.331938339165134 29.55655558887743 31.991870970533327 19.119635101424105 42-43 19.213581661380637 29.494208417233196 31.736482241790252 19.555727679595904 44-45 19.35076763442352 29.5334464284881 31.460687979970363 19.655097957118016 46-47 19.562578261002354 29.409975669099758 30.91122954185288 20.116216528045012 48-49 19.250976459769177 29.42066497673803 31.140667895088686 20.187690668404105 50-51 19.19175766049088 29.634110234376788 30.850193808149868 20.323938296982462 52-53 19.237657243108792 30.00760128543166 30.783806207942664 19.970935263516886 54-55 19.529409768543754 29.571128309434076 30.555108983590422 20.344352938431747 56-57 19.575261209350938 29.858290321595454 30.12925578664821 20.4371926824054 58-59 19.384309384576678 29.670597289817447 30.408623300408195 20.53647002519768 60-61 19.51791222551385 29.78677376334891 30.245820141370334 20.449493869766904 62-63 19.210179907189286 29.65636959008956 30.474391786095023 20.659058716626134 64-65 19.254776435051728 29.610736717512076 30.21901495241065 20.91547189502555 66-67 19.16368591092348 29.683745774905994 29.8369690634884 21.315599250682123 68-69 19.238637385686577 29.334491811179426 30.06818501932022 21.35868578381378 70-71 19.19719872152433 29.18245849938176 30.221569929098468 21.39877284999544 72-73 19.26467743741665 28.69841560706784 30.219733140586452 21.81717381492906 74-75 19.072740348204988 29.107170510979252 29.740447910324598 22.079641230491166 76-77 18.799508212236045 28.806039635852976 30.35766652902155 22.036785622889436 78-79 18.701023946394294 28.629510634072826 30.166193984633168 22.50327143489972 80-81 18.711874446029896 28.61962847884741 30.00524852865115 22.663248546471543 82-83 18.677933751138823 28.680781074760702 30.10473992393275 22.53654525016772 84-85 18.62002665967508 28.27414664857678 30.055135126879517 23.05069156486862 86-87 18.301432717267314 28.45525903068339 30.123163711905597 23.1201445401437 88-89 18.688139303262925 28.071309033264356 29.905658855424804 23.334892808047908 90-91 18.49182929235286 27.808748374450282 30.30733231645873 23.392090016738123 92-93 18.39201258937023 27.839555960794414 30.20706357351938 23.561367876315973 94-95 18.17643739366193 27.595921709725186 30.299592255830827 23.928048640782055 96-97 18.295171538185063 27.329924734436084 30.163044977295574 24.211858750083277 98-99 18.130611704430287 27.27999232674486 29.908633939893964 24.68076202893089 100-101 17.870453372046605 27.121590015777468 30.505281994129373 24.502674618046555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2341.0 1 1456.5 2 663.5 3 775.5 4 687.5 5 703.5 6 1223.5 7 2242.5 8 3034.0 9 3047.5 10 2388.0 11 1718.0 12 1540.5 13 1518.0 14 1463.5 15 1457.5 16 1544.0 17 1641.0 18 1788.0 19 2018.5 20 2279.0 21 2636.5 22 3082.5 23 3621.0 24 4389.0 25 5779.5 26 8159.0 27 10866.0 28 12672.0 29 14984.0 30 18904.0 31 23061.5 32 27723.5 33 33382.5 34 40045.0 35 45616.0 36 50085.0 37 53992.0 38 54107.0 39 52343.5 40 51469.0 41 50041.5 42 48724.5 43 48443.0 44 48346.5 45 47780.5 46 46065.0 47 44083.5 48 43818.0 49 41729.0 50 37090.5 51 32267.0 52 27290.5 53 23091.0 54 19445.5 55 16305.5 56 13272.5 57 10633.5 58 8349.5 59 6151.0 60 4451.0 61 3196.0 62 2170.5 63 1392.0 64 865.5 65 538.5 66 392.0 67 331.0 68 246.5 69 181.5 70 132.5 71 106.5 72 111.5 73 122.5 74 119.5 75 97.0 76 53.0 77 17.5 78 8.0 79 3.5 80 2.5 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.635219741624164E-4 2 0.04937442099583626 3 0.0 4 8.483577490693515E-5 5 0.04818672014713917 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006489936780380539 26-27 0.001696715498138703 28-29 0.0 30-31 0.0016542976106852353 32-33 3.817609870812082E-4 34-35 0.012386023136412534 36-37 0.022735987675058623 38-39 0.004623549732427966 40-41 8.05939861615884E-4 42-43 9.756114114297543E-4 44-45 0.008016980728705372 46-47 0.0 48-49 0.0 50-51 4.2417887453467573E-4 52-53 0.0 54-55 0.0013149545110574948 56-57 0.0 58-59 0.02260873401269822 60-61 0.0 62-63 0.009544024677030204 64-65 0.01488867849616712 66-67 0.006447518892927072 68-69 0.01611879723231768 70-71 4.2417887453467576E-5 72-73 0.0 74-75 0.023372255986860636 76-77 0.05068937550689375 78-79 0.031049893615938268 80-81 0.02735953740748659 82-83 0.038048845045760414 84-85 0.016033961457410744 86-87 0.00962886045193714 88-89 0.08492061068184209 90-91 0.025493150359534013 92-93 0.02489929993518547 94-95 0.026553597545870705 96-97 0.03847302392029509 98-99 0.053446538191369145 100-101 0.014294828071818573 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1178748.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.18186004488578 #Duplication Level Percentage of deduplicated Percentage of total 1 76.32849774132428 42.11948479798137 2 13.77501140953279 15.202615034350861 3 4.630726548562275 7.665963129267013 4 1.9888128524205584 4.389855699109653 5 1.0020373240664668 2.7647141688193813 6 0.5573218574989046 1.8452434044276198 7 0.3382962168466631 1.3067470144222824 8 0.2195765308194116 0.9693313114254655 9 0.14528586321834336 0.7215429753553538 >10 0.8435814744275016 9.031541230141704 >50 0.0993476914793508 3.8588986968019796 >100 0.06736152982199965 6.590925590659389 >500 0.002761973320974414 0.9566319464589598 >1k 9.206577736581381E-4 0.9353791036052512 >5k 4.6032888682906903E-4 1.64112589717362 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7953 0.6746989178348552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6417 0.5443911675778029 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5012 0.425196903833559 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4095 0.34740249824389946 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1829 0.1551646323047844 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 0.12428441023865999 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1400 0.1187700848697092 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 1180 0.10010621439018347 RNA PCR Primer, Index 39 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.483577490693515E-5 0.0 12-13 0.0 0.0 0.0 2.96925212174273E-4 0.0 14-15 0.0 0.0 0.0 4.241788745346758E-4 0.0 16-17 0.0 0.0 0.0 6.362683118020136E-4 0.0 18-19 0.0 0.0 0.0 7.635219741624164E-4 0.0 20-21 0.0 0.0 0.0 0.0011452829612436247 0.0 22-23 0.0 0.0 0.0 0.0024602374723011193 0.0 24-25 0.0 0.0 0.0 0.00576883269367159 0.0 26-27 0.0 0.0 0.0 0.012004262149331325 0.0 28-29 0.0 0.0 0.0 0.025111389372452806 0.0 30-31 0.0 0.0 0.0 0.05938504243485461 0.0 32-33 0.0 0.0 0.0 0.12076372558002219 0.0 34-35 0.0 0.0 0.0 0.2170099122119401 0.0 36-37 0.0 0.0 0.0 0.3626729377271478 0.0 38-39 0.0 0.0 0.0 0.5593222639614235 0.0 40-41 0.0 0.0 0.0 0.7677213450203098 0.0 42-43 0.0 0.0 0.0 0.97531448621758 0.0 44-45 0.0 0.0 0.0 1.2319002874236054 0.0 46-47 0.0 0.0 0.0 1.524795800289799 0.0 48-49 0.0 0.0 0.0 1.8281261134695457 0.0 50-51 0.0 0.0 0.0 2.1236515353578542 0.0 52-53 0.0 0.0 0.0 2.418413435272001 0.0 54-55 0.0 0.0 0.0 2.7512241802319073 0.0 56-57 0.0 0.0 0.0 3.1462619660860507 0.0 58-59 0.0 0.0 0.0 3.557715474384686 0.0 60-61 0.0 0.0 0.0 3.979815872434142 0.0 62-63 0.0 0.0 0.0 4.395468751590671 0.0 64-65 0.0 0.0 0.0 4.849043222130599 0.0 66-67 0.0 0.0 0.0 5.358100289459664 0.0 68-69 0.0 0.0 0.0 5.904442679860326 0.0 70-71 0.0 0.0 0.0 6.49337262926427 0.0 72-73 0.0 0.0 0.0 7.068050168483849 0.0 74-75 0.0 0.0 0.0 7.677807300627445 0.0 76-77 0.0 0.0 0.0 8.345337595482665 0.0 78-79 0.0 0.0 0.0 9.068392905014473 0.0 80-81 0.0 0.0 0.0 9.82003787068992 0.0 82-83 0.0 0.0 0.0 10.563453766199391 0.0 84-85 0.0 0.0 0.0 11.320740310906148 0.0 86-87 0.0 0.0 0.0 12.136521122411237 0.0 88-89 0.0 0.0 0.0 12.975292428916104 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGTGC 45 9.644086E-4 42.205387 8 GGTATCA 4705 0.0 34.949615 1 GTATCAA 6880 0.0 28.667217 1 CATTCCG 250 0.0 26.589394 9 AGTCGAA 110 9.50256E-5 25.898758 6 GTACATG 5405 0.0 25.851011 1 TACATGG 5440 0.0 24.722649 2 GCGTAAA 100 0.0016485297 23.762707 1 TAGTCGA 120 1.70908E-4 23.75363 5 GTGTACG 120 1.7154844E-4 23.740532 9 ACATGGG 5410 0.0 23.621908 3 CATGGGG 2195 0.0 23.591305 4 TCAACGC 8350 0.0 23.270023 4 ATCAACG 8410 0.0 23.047516 3 TATCAAC 8455 0.0 23.045052 2 CAACGCA 8475 0.0 22.926805 5 AACGCAG 8715 0.0 22.283136 6 GAGTACT 5685 0.0 22.007492 12-13 AGTACTT 5840 0.0 20.651009 12-13 GTACTTT 6220 0.0 20.076397 14-15 >>END_MODULE