FastQCFastQC Report
Thu 26 May 2016
SRR938414_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938414_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167452
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84310.7221710185943405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63930.5476028136488695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49730.4259704039223882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39540.3386863014496528No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.12077584346080182No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13200.11306674706968681No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.10176007236271811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48550.034.227141
AGCATCG300.00394443831.67869818-19
CATTCCG1805.456968E-1231.6691939
GTATCAA71300.030.318011
GTACATG51300.025.6168731
TATCAAC85750.025.0527822
TCAACGC86350.024.9348144
CAACGCA87050.024.7343035
ATCAACG87200.024.6917573
TACATGG52050.024.3804742
AACGCAG89450.024.1717576
CATGGGT11250.023.237024
GAGTACT56350.023.13872712-13
ACATGGG52900.022.9115683
ACGCAGA98650.021.8627937
CGCAGAG98850.021.818568
CATGGGG20800.021.2515144
AGTACTT58700.020.71538212-13
GTACTTT62850.020.5216114-15
GCAGAGT102400.020.5045669
Produced by FastQC (version 0.11.5)