##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938414_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1167452 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.266485474349267 31.0 31.0 34.0 28.0 34.0 2 31.371025104244115 31.0 31.0 34.0 28.0 34.0 3 31.416262081867178 31.0 31.0 34.0 28.0 34.0 4 35.05052284804857 37.0 35.0 37.0 32.0 37.0 5 35.09406810729692 37.0 35.0 37.0 32.0 37.0 6 35.14843950757719 37.0 35.0 37.0 32.0 37.0 7 35.0935618766339 37.0 35.0 37.0 32.0 37.0 8 35.07158067312403 37.0 35.0 37.0 32.0 37.0 9 36.60907600483789 39.0 37.0 39.0 32.0 39.0 10-11 36.49491799234572 39.0 37.0 39.0 32.0 39.0 12-13 36.390352665462906 39.0 37.0 39.0 32.0 39.0 14-15 37.424104374312606 40.0 37.0 41.0 32.0 41.0 16-17 37.318275612187904 39.5 37.0 41.0 32.0 41.0 18-19 37.36975438818898 39.5 37.0 41.0 32.0 41.0 20-21 37.47433213528265 40.0 37.0 41.0 32.0 41.0 22-23 37.39285897835629 40.0 37.0 41.0 32.0 41.0 24-25 37.312809862846606 40.0 37.0 41.0 31.5 41.0 26-27 37.155858656287364 39.0 36.0 41.0 31.0 41.0 28-29 36.992515752253624 39.0 36.0 41.0 31.0 41.0 30-31 36.785615168760685 39.0 36.0 41.0 30.5 41.0 32-33 36.49904878316197 39.0 35.0 40.5 30.0 41.0 34-35 36.286772818068755 39.0 35.0 40.0 30.0 41.0 36-37 36.02520703206642 38.0 35.0 40.0 30.0 41.0 38-39 35.81428272854045 38.0 35.0 40.0 29.0 41.0 40-41 35.728846667785916 38.0 35.0 40.0 28.5 41.0 42-43 35.55526865344356 38.0 35.0 40.0 27.0 41.0 44-45 35.24359588231465 38.0 35.0 40.0 26.5 41.0 46-47 34.86408563264271 38.0 34.0 40.0 24.5 41.0 48-49 34.52889840438836 38.0 34.0 40.0 23.5 41.0 50-51 33.82070997351497 37.5 33.5 39.5 22.5 40.5 52-53 33.92631474356119 38.0 33.5 39.5 23.0 40.5 54-55 34.128687946056885 38.0 34.0 40.0 22.5 41.0 56-57 33.919738456056436 38.0 33.5 40.0 20.0 41.0 58-59 33.68735374131013 38.0 33.0 40.0 19.0 41.0 60-61 33.34609431479838 38.0 33.0 40.0 16.0 41.0 62-63 32.9312549894985 37.5 32.0 40.0 12.5 41.0 64-65 32.54253708075365 37.0 31.5 39.5 8.0 41.0 66-67 32.08487372500112 36.0 31.0 39.0 7.5 40.5 68-69 31.588617347865267 36.0 31.0 39.0 4.0 40.0 70-71 31.04978191822876 35.0 30.0 38.5 2.0 40.0 72-73 30.553551666364015 35.0 29.5 38.0 2.0 40.0 74-75 30.005244755244757 35.0 29.0 37.0 2.0 39.0 76-77 29.32179866923865 34.0 28.5 36.5 2.0 39.0 78-79 28.586786437472377 34.0 26.5 36.0 2.0 38.5 80-81 27.953341978942177 34.0 26.0 35.5 2.0 37.5 82-83 27.324095123396937 33.0 26.0 35.0 2.0 37.0 84-85 26.69009646649284 33.0 25.0 35.0 2.0 36.0 86-87 26.065695634595684 32.0 23.0 35.0 2.0 36.0 88-89 25.32971805264799 32.0 20.0 34.5 2.0 35.5 90-91 24.56771284815136 31.0 16.0 34.0 2.0 35.0 92-93 23.807266594258266 31.0 4.5 34.0 2.0 35.0 94-95 22.932332978143855 30.5 2.0 34.0 2.0 35.0 96-97 21.784686650928688 29.5 2.0 34.0 2.0 35.0 98-99 20.427026978411103 29.0 2.0 34.0 2.0 35.0 100-101 16.868780900628032 21.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6012100004689493 1101 2 0.6441623849100964 1101 3 0.5628359725312215 1101 4 0.49255371823468863 1101 5 0.4185088790427045 1101 6 0.6027484002218486 1101 7 0.4560031576476149 1101 8 0.5717208874208026 1101 9 0.6439118080270418 1101 10-11 0.6255976048082914 1101 12-13 0.5628884302427508 1101 14-15 0.7463458775756777 1101 16-17 0.7189154114608698 1101 18-19 0.7921517252024586 1101 20-21 0.7613318112518144 1101 22-23 0.8142223335482157 1101 24-25 0.8727332308685547 1101 26-27 0.8633404535173455 1101 28-29 0.9430443510731763 1101 30-31 0.8390292034412283 1101 32-33 0.7516767377056368 1101 34-35 0.7552402855757165 1101 36-37 0.7488211097694233 1101 38-39 1.0055651359338924 1101 40-41 0.7521784827837692 1101 42-43 0.6915223211499253 1101 44-45 0.7148951624232112 1101 46-47 0.7972477105583593 1101 48-49 0.6787883752405932 1101 50-51 0.5856620295427248 1101 52-53 0.5937283544677499 1101 54-55 0.6589831559291071 1101 56-57 0.4481463482627319 1101 58-59 0.3518825333190563 1101 60-61 0.5128106315356362 1101 62-63 0.4028445609217215 1101 64-65 0.19150588794879297 1101 66-67 0.26102148085644217 1101 68-69 0.34163568023598145 1101 70-71 0.6431692575636028 1101 72-73 0.6348751536493751 1101 74-75 0.5014480770715757 1101 76-77 0.5206666670938525 1101 78-79 0.4297162092019704 1101 80-81 0.5493771260512617 1101 82-83 0.7556397394563348 1101 84-85 0.9667019022475039 1101 86-87 0.9853810115001416 1101 88-89 1.0155035359172224 1101 90-91 1.1409946896920609 1101 92-93 0.9767830180353343 1101 94-95 0.8324073615754806 1101 96-97 0.989474434705766 1101 98-99 1.4428438171990976 1101 100-101 1.7659630084295301 1102 1 0.5148852141441651 1102 2 0.6197321854798972 1102 3 0.5127172640791784 1102 4 0.48683197084627494 1102 5 0.6035990974662582 1102 6 0.30512256926268577 1102 7 0.37637352801798585 1102 8 0.43207226443885105 1102 9 0.4440779998598998 1102 10-11 0.5797998840105691 1102 12-13 0.4256377179920392 1102 14-15 0.5856265043563056 1102 16-17 0.7487587163041738 1102 18-19 0.7796398543572494 1102 20-21 0.5924452965319631 1102 22-23 0.6033367684959785 1102 24-25 0.6163941995295161 1102 26-27 0.6275735969171592 1102 28-29 0.46783067585949567 1102 30-31 0.4925311027764607 1102 32-33 0.6879065572687892 1102 34-35 0.6432626027647004 1102 36-37 0.6115229141378933 1102 38-39 0.5050903021257298 1102 40-41 0.5749467638853787 1102 42-43 0.6919021881964582 1102 44-45 0.7709849060129557 1102 46-47 0.49422064503129093 1102 48-49 0.3508325347847503 1102 50-51 0.6159682973489993 1102 52-53 0.5067031882759139 1102 54-55 0.4895624531750684 1102 56-57 0.446341979791697 1102 58-59 0.6082096938128885 1102 60-61 0.6629768233684885 1102 62-63 0.33165510223226136 1102 64-65 0.19752440646730918 1102 66-67 0.3944853934870238 1102 68-69 0.45034888061584866 1102 70-71 0.42752348358449765 1102 72-73 0.3757227319969516 1102 74-75 0.38723867536217327 1102 76-77 0.39795774021825636 1102 78-79 0.009761318413747944 1102 80-81 0.14360789528202744 1102 82-83 0.09272425987418842 1102 84-85 -0.0875003769547753 1102 86-87 0.16937911216490775 1102 88-89 0.538331171244856 1102 90-91 0.7967045662352668 1102 92-93 0.7650664938188143 1102 94-95 0.9018399351747242 1102 96-97 1.0413975116288405 1102 98-99 1.1291211201430684 1102 100-101 1.1776439201104445 1103 1 0.52519775717046 1103 2 0.610400642040549 1103 3 0.503117236542348 1103 4 0.5761235858813833 1103 5 0.4640355562298524 1103 6 0.44995252933427565 1103 7 0.3623435128727266 1103 8 0.2648617453695934 1103 9 0.4554266161341687 1103 10-11 0.40638325176494305 1103 12-13 0.479854295164003 1103 14-15 0.7581258159355428 1103 16-17 0.7323157863854135 1103 18-19 0.6236161726830147 1103 20-21 0.5342169473649534 1103 22-23 0.6461651451998307 1103 24-25 0.654350278284852 1103 26-27 0.6714621415956614 1103 28-29 0.6811068702695238 1103 30-31 0.7209129697735719 1103 32-33 0.927532056373849 1103 34-35 0.9019418675313275 1103 36-37 0.9234222670223815 1103 38-39 1.0290989762273384 1103 40-41 0.9404465573591523 1103 42-43 0.9568891082019704 1103 44-45 1.1842869162015788 1103 46-47 0.9310872979295439 1103 48-49 1.014034723962773 1103 50-51 1.1613128519407638 1103 52-53 1.1542162682704102 1103 54-55 1.114101211264014 1103 56-57 1.4686158335711283 1103 58-59 1.3114607608650672 1103 60-61 0.9920247374535833 1103 62-63 0.9863552949900765 1103 64-65 0.8345941434905839 1103 66-67 0.9943872935283338 1103 68-69 0.9919157262719125 1103 70-71 1.2281843675167572 1103 72-73 1.2756624951802102 1103 74-75 1.2043786313032463 1103 76-77 1.2348484878432053 1103 78-79 1.1686608778244612 1103 80-81 1.165038433627064 1103 82-83 1.306812790784278 1103 84-85 1.1268153328070305 1103 86-87 1.4260912146019145 1103 88-89 1.1755971081855172 1103 90-91 1.2345625450319098 1103 92-93 1.4538056511918 1103 94-95 1.414956071757402 1103 96-97 1.365774490838536 1103 98-99 1.454327646371901 1103 100-101 1.334982485441575 1104 1 0.4936695393664827 1104 2 0.47424454744608724 1104 3 0.5854500892526389 1104 4 0.5320313258741223 1104 5 0.29988562145726405 1104 6 0.4914217972505668 1104 7 0.37362262407974356 1104 8 0.3303474525557277 1104 9 0.6059000461806292 1104 10-11 0.5976074673194773 1104 12-13 0.5078252485304944 1104 14-15 0.6401058847921561 1104 16-17 0.47709595855962306 1104 18-19 0.36335675067309836 1104 20-21 0.7534525768993987 1104 22-23 0.7324594597888563 1104 24-25 0.7243708437198819 1104 26-27 0.4424873563230065 1104 28-29 0.681296912544731 1104 30-31 0.7232662244416659 1104 32-33 0.8176974299356559 1104 34-35 0.7890812776401006 1104 36-37 0.8225143145092417 1104 38-39 0.7258466785549302 1104 40-41 0.7735322226951098 1104 42-43 0.8585297656419826 1104 44-45 0.8677001898984216 1104 46-47 0.8034982273629652 1104 48-49 0.6486520891676335 1104 50-51 0.48226728102924454 1104 52-53 0.41077701804236 1104 54-55 0.6595907928603992 1104 56-57 0.737546905033561 1104 58-59 0.5351458317978341 1104 60-61 0.49429461873641856 1104 62-63 0.4225583075483996 1104 64-65 0.4217414005062068 1104 66-67 0.7177740185236701 1104 68-69 0.6594786568300535 1104 70-71 0.7347720910309938 1104 72-73 0.7089229176707512 1104 74-75 0.4968851622184225 1104 76-77 0.5270950899690625 1104 78-79 0.7346672599753639 1104 80-81 1.176176545726669 1104 82-83 1.0109349507761713 1104 84-85 1.27652532420813 1104 86-87 1.1355913666357367 1104 88-89 1.2461600093177623 1104 90-91 1.237952245312762 1104 92-93 1.398916537794186 1104 94-95 1.3156616394647678 1104 96-97 1.2944342554967214 1104 98-99 0.8740492936992119 1104 100-101 0.7605619304690876 1105 1 0.41004190930085826 1105 2 0.6512848920326064 1105 3 0.4917058680677826 1105 4 0.5938832528975553 1105 5 0.43721733108449 1105 6 0.49201715615726727 1105 7 0.4696783713228285 1105 8 0.5604198427864304 1105 9 0.6913002220821198 1105 10-11 0.6023782315889221 1105 12-13 0.575993843348158 1105 14-15 0.6343800656098679 1105 16-17 0.6731889157343716 1105 18-19 0.6788563785737765 1105 20-21 0.8712558378425044 1105 22-23 0.8901957428549565 1105 24-25 0.7750124331477579 1105 26-27 0.9343755912191511 1105 28-29 0.9364916445204656 1105 30-31 0.8503302480756076 1105 32-33 0.8025789219411621 1105 34-35 0.5792668762689672 1105 36-37 0.6058961335111164 1105 38-39 0.5888509858864097 1105 40-41 0.7537929177315306 1105 42-43 0.8181130144072881 1105 44-45 0.7536416011696545 1105 46-47 0.8520910154016619 1105 48-49 0.9203838168360292 1105 50-51 0.6635822574629557 1105 52-53 0.6222658663386014 1105 54-55 0.7072262708388877 1105 56-57 0.8386496720993861 1105 58-59 0.8902965883997851 1105 60-61 1.0050708404625084 1105 62-63 1.1730724811496458 1105 64-65 1.15461130105421 1105 66-67 1.1039939404955703 1105 68-69 1.044722099989066 1105 70-71 1.3444038254648412 1105 72-73 1.2387213074955277 1105 74-75 1.2635563391798392 1105 76-77 1.2061842359447503 1105 78-79 1.233514879667311 1105 80-81 1.4196050462791838 1105 82-83 1.4723538895038182 1105 84-85 1.3440523295979325 1105 86-87 1.2693791121649056 1105 88-89 1.123623194036881 1105 90-91 1.3447458767765816 1105 92-93 1.4457288869812075 1105 94-95 0.8836419294767204 1105 96-97 0.9380499332812633 1105 98-99 0.911172402194353 1105 100-101 1.0296382221047473 1106 1 0.32823577400635173 1106 2 0.40080453690108797 1106 3 0.26782062325230527 1106 4 0.33000595878770156 1106 5 0.4284914316609161 1106 6 0.10339991637724211 1106 7 0.2742632782333132 1106 8 0.2648457355844158 1106 9 0.324116759549824 1106 10-11 0.2493304610992908 1106 12-13 0.2356506378886749 1106 14-15 0.49888016499368604 1106 16-17 0.07845294541701264 1106 18-19 -0.03261251429046297 1106 20-21 0.23929068843549572 1106 22-23 0.35726657039089815 1106 24-25 0.4012563872986803 1106 26-27 0.24877299398864494 1106 28-29 0.3070210290033444 1106 30-31 0.615625418021942 1106 32-33 0.6922562558045371 1106 34-35 0.5405601910506519 1106 36-37 0.3882455670233398 1106 38-39 0.5770110600929996 1106 40-41 0.651281819871599 1106 42-43 0.12316833806726635 1106 44-45 0.6156769818096777 1106 46-47 0.3557516527832334 1106 48-49 0.736265351322217 1106 50-51 0.548989399846846 1106 52-53 0.9167699410752306 1106 54-55 0.6444289475764435 1106 56-57 0.14427479632916373 1106 58-59 -0.23956162401657366 1106 60-61 0.8062800102763319 1106 62-63 0.6887718119652533 1106 64-65 1.0468030455715294 1106 66-67 0.35323862346118773 1106 68-69 0.0652713612360003 1106 70-71 0.1318947152813088 1106 72-73 0.728295944744584 1106 74-75 0.9322106822527836 1106 76-77 0.9120511941372946 1106 78-79 1.0962061935214145 1106 80-81 1.0266182311993433 1106 82-83 0.8479783511747883 1106 84-85 1.3080853250262763 1106 86-87 1.1566745471347595 1106 88-89 0.6904521876100631 1106 90-91 0.9094414310070178 1106 92-93 0.544728423190044 1106 94-95 0.36595492225482573 1106 96-97 0.3127368742472747 1106 98-99 0.5511280107546099 1106 100-101 0.48944409237573083 1107 1 0.27889061026876405 1107 2 0.23256637728760055 1107 3 0.294627982744867 1107 4 0.37218885090513965 1107 5 0.35231751787341636 1107 6 0.21341297887759225 1107 7 0.17445422460861693 1107 8 0.15507937751218748 1107 9 0.09163134790993865 1107 10-11 0.27316203755484736 1107 12-13 0.2420407103884088 1107 14-15 0.18289778225672393 1107 16-17 0.27579759292915185 1107 18-19 0.28602015850245976 1107 20-21 0.365142745636696 1107 22-23 0.4773709848315235 1107 24-25 0.38551365010592775 1107 26-27 0.48960143601453154 1107 28-29 0.5592827239340608 1107 30-31 0.46136848320325186 1107 32-33 0.36109762913535803 1107 34-35 0.3714542878272411 1107 36-37 0.4258287762914321 1107 38-39 0.4565251807129229 1107 40-41 0.5079857631495486 1107 42-43 0.5606690510858385 1107 44-45 0.5774295957357864 1107 46-47 0.6041605518255082 1107 48-49 0.3538310631144057 1107 50-51 0.4977533108393075 1107 52-53 0.5123568551878677 1107 54-55 0.4368981166531114 1107 56-57 0.24316869060847068 1107 58-59 0.6128945110804835 1107 60-61 0.6581938947445067 1107 62-63 0.507866164287698 1107 64-65 0.3063115048239453 1107 66-67 0.2555597128394851 1107 68-69 0.31812236602178245 1107 70-71 0.0683715336212849 1107 72-73 0.0046747800715145615 1107 74-75 0.32783224906833297 1107 76-77 0.26855192712039155 1107 78-79 0.2512930819653789 1107 80-81 0.2943559943745022 1107 82-83 0.2934723589666568 1107 84-85 0.18541475672194707 1107 86-87 0.47102554336431623 1107 88-89 0.760731220300535 1107 90-91 0.6892327732502288 1107 92-93 0.5004417697570034 1107 94-95 0.8959501878114686 1107 96-97 1.0381291770691163 1107 98-99 0.5972410612762573 1107 100-101 0.4388347466986211 1108 1 0.25316698850501496 1108 2 0.19499150575569146 1108 3 0.11242285929548501 1108 4 0.22191800416130292 1108 5 0.09965132762062723 1108 6 0.1712339854564746 1108 7 0.32137017210853003 1108 8 0.04679250362367071 1108 9 0.11535294036877985 1108 10-11 0.20580970767278473 1108 12-13 0.21328949662404284 1108 14-15 0.1983446384706511 1108 16-17 0.10806831420521235 1108 18-19 0.1719624487803415 1108 20-21 0.3621315189982539 1108 22-23 0.2871986575679202 1108 24-25 0.19410028287810377 1108 26-27 0.20458637988134143 1108 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1623.0 9 2036.0 10 2753.0 11 3906.0 12 5262.0 13 6959.0 14 8483.0 15 10901.0 16 12853.0 17 15454.0 18 13186.0 19 10486.0 20 10736.0 21 11400.0 22 12472.0 23 13860.0 24 15645.0 25 18090.0 26 20655.0 27 24448.0 28 28218.0 29 33272.0 30 39252.0 31 46846.0 32 55772.0 33 68417.0 34 86444.0 35 112322.0 36 145578.0 37 186983.0 38 123613.0 39 8407.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.046428911601694 21.729609318218287 15.345215651984386 21.878746118195632 2 14.541194537903706 22.40174208443385 41.366106825220804 21.69095655244164 3 17.767446643500172 26.944378982188077 31.766238329269914 23.52193604504184 4 11.459460849220326 19.077247425728576 40.22959544408592 29.233696280965177 5 11.672273336961599 39.40887839453532 36.0039082420196 12.914940026483482 6 27.856500012856888 39.77500449991 19.000077141314318 13.368418345918794 7 25.326791365081757 35.00478565656894 22.85924342925497 16.809179549094324 8 23.99460866555047 36.40401762380276 21.52232365486548 18.07905005578129 9 25.431838942369488 17.475102529079184 22.684590903404153 34.408467625147175 10-11 22.65977411393707 28.440263590868582 29.759546171248374 19.14041612394598 12-13 23.968430041075564 26.922554542841 29.24360033950431 19.86541507657913 14-15 21.186298715959826 28.567462556504235 28.15724318875189 22.088995538784044 16-17 19.224695542796233 31.34231931569211 29.32994138347341 20.103043758038243 18-19 18.853506623267062 29.915307358409642 32.40394366848917 18.827242349834126 20-21 20.141799115919447 29.078117206661968 32.10838128244814 18.671702394970442 22-23 19.56326125911497 29.49381223224975 31.5942082391021 19.348718269533183 24-25 19.232363197970958 29.608964405607985 32.2047540047066 18.953918391714456 26-27 19.225262880039974 29.653905502451288 32.05193195778836 19.06889965972038 28-29 18.7648037760555 29.786825624800763 32.295198006396255 19.153172592747477 30-31 19.432110446346464 29.525148929277556 32.217488595158336 18.825252029217648 32-33 19.124774558404425 29.60583232244372 32.054077702310394 19.215315416841456 34-35 19.428571183718663 29.810255021501746 31.89774115977451 18.863432635005083 36-37 19.419869818636474 29.34445272366647 31.683920917671067 19.55175654002599 38-39 19.054006235739752 29.85722400768372 31.835259261290176 19.253510495286353 40-41 19.41536165378361 29.46491758670146 32.01052806541657 19.109192694098354 42-43 19.1734839044363 29.41135648930925 31.75405596553463 19.661103640719823 44-45 19.427876358247566 29.428475978773434 31.501149986508537 19.642497676470462 46-47 19.555445903333 29.44299240915356 30.97585338127191 20.025708306241523 48-49 19.32904775529715 29.400955688470916 31.222640462047448 20.047356094184487 50-51 19.26656038596855 29.660324376000663 30.895816075569517 20.177299162461267 52-53 19.263891815766073 29.851791505831194 30.973784889089917 19.910531789312817 54-55 19.569652513787013 29.468458531884135 30.792611233067408 20.169277721261444 56-57 19.601312539324997 29.71502682773157 30.45044975587985 20.233210877063584 58-59 19.557353785871147 29.353592265363282 30.722025356360998 20.36702859240457 60-61 19.578400725766866 29.418187176234046 30.716163647064164 20.28724845093492 62-63 19.456520156278508 29.157968584970227 30.95944588115672 20.42606537759454 64-65 19.498821280007675 29.153060560166118 30.740049410297352 20.608068749528854 66-67 19.467059787452655 29.313167904520306 30.293328696289777 20.92644361173726 68-69 19.594894418983166 28.989077825844863 30.547821989977297 20.868205765194674 70-71 19.624326584326035 28.995299798489356 30.622121276508246 20.758252340676364 72-73 19.805494941394887 28.592363558166888 30.66669579573328 20.935445704704943 74-75 19.71430248193668 29.02678044762162 30.24952161708205 21.009395453359648 76-77 19.63235908276097 28.877052533287074 30.429899576589715 21.06068880736224 78-79 19.584843111556548 28.713112156177456 30.312706122204236 21.389338610061763 80-81 19.79880475272053 28.76140326981896 30.027621836541602 21.41217014091891 82-83 19.971697414176734 28.827477150844473 30.08387553543841 21.116949899540383 84-85 19.98521491905468 28.503811484221682 30.04348150108832 21.46749209563532 86-87 19.877380864167655 28.652521577635586 30.004039047264563 21.4660585109322 88-89 20.51509217414103 28.333882409692645 29.91989197261787 21.23113344354845 90-91 20.501718466293468 28.30825112315118 29.936531140185892 21.25349927036946 92-93 20.726582958465613 28.265118903142977 29.856377220665536 21.15192091772587 94-95 20.6486231752118 28.15255925541144 29.779043987704434 21.419773581672324 96-97 21.023872360844532 27.800460995338632 29.548901494378942 21.626765149437894 98-99 21.084653727461056 27.774755355114195 29.278993696035478 21.86159722138927 100-101 21.5817827152087 27.44526542788741 29.615137389061104 21.357814467842783 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2439.0 1 1511.5 2 677.0 3 774.5 4 641.0 5 632.5 6 1166.5 7 2203.5 8 3222.0 9 3286.5 10 2425.5 11 1772.0 12 1629.0 13 1583.0 14 1593.0 15 1593.5 16 1666.0 17 1815.5 18 1966.0 19 2250.0 20 2551.0 21 2929.0 22 3371.5 23 3858.0 24 4634.5 25 5971.5 26 8248.0 27 11142.0 28 13066.5 29 15316.0 30 19172.5 31 23205.5 32 27792.0 33 33184.0 34 39549.0 35 45020.0 36 49433.5 37 53105.5 38 52715.0 39 51022.5 40 50414.0 41 49195.5 42 48033.5 43 47560.5 44 47092.0 45 46031.5 46 44217.5 47 42462.5 48 42053.5 49 40261.0 50 35916.0 51 31183.0 52 26787.5 53 23065.5 54 19903.0 55 16745.5 56 13693.0 57 11049.0 58 8636.0 59 6644.0 60 4975.5 61 3568.5 62 2459.5 63 1641.0 64 1111.5 65 756.5 66 575.5 67 484.0 68 381.5 69 291.5 70 235.0 71 208.0 72 182.5 73 160.5 74 169.5 75 143.5 76 81.0 77 48.5 78 33.5 79 23.0 80 20.5 81 15.5 82 7.5 83 4.5 84 3.5 85 2.5 86 1.0 87 1.5 88 1.5 89 1.5 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1780801266347567 2 0.04916690365000017 3 0.04599760846698622 4 0.05285013859242178 5 0.058846102452177904 6 0.06527034944477375 7 0.03623275303824054 8 0.033748710867770156 9 0.03991598798066216 10-11 0.04214306027142872 12-13 0.03910225002826669 14-15 0.03370588255448618 16-17 0.0321212349629792 18-19 0.04004447292051408 20-21 0.05053740967508729 22-23 0.047368114492073336 24-25 0.067968533181664 26-27 0.05289296690570576 28-29 0.04454144581533117 30-31 0.05336407835182946 32-33 0.049295388589852086 34-35 0.04882427714372839 36-37 0.04638306328654197 38-39 0.026467897609494865 40-41 0.02449779519843214 42-43 0.021542641581838055 44-45 0.004025861448693394 46-47 0.010707078320993069 48-49 0.010792734947561014 50-51 0.008822632536498289 52-53 0.0094222289224739 54-55 0.011949099406228266 56-57 0.008265864463806649 58-59 0.011263846393684709 60-61 0.011563644586672514 62-63 0.004197174701829283 64-65 0.007195156631707342 66-67 0.0035547500025696985 68-69 0.008736975909930344 70-71 0.022955975920209138 72-73 0.01995799399033108 74-75 0.020429105436454775 76-77 0.01237738253906799 78-79 0.005310710847212562 80-81 0.006724045185583646 82-83 0.005781822293336257 84-85 0.005053740967508729 86-87 0.008351521090374594 88-89 0.02916608134638512 90-91 0.03623275303824054 92-93 0.022484864474085445 94-95 0.03122184038401579 96-97 0.035290530145993156 98-99 0.021414156641986138 100-101 0.12321705731798824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1167452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.00210428072552 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6477616714928 44.26085807963354 2 13.158029555992604 15.000707457591176 3 4.27545568222766 7.311299119378846 4 1.85184081571356 4.222352931544326 5 0.9377496727977169 2.6726852319015846 6 0.5297073083653772 1.8116658737823395 7 0.30492554904868374 1.2166978561311388 8 0.20968557802424595 0.956201534776182 9 0.1415295196644151 0.7260732394840784 >10 0.7834210764306392 8.66884595498511 >50 0.09692812692862834 3.8763493218232306 >100 0.05995274548977886 6.172928481463645 >500 0.001958253585450343 0.7275346343870867 >1k 7.531744559424396E-4 1.1029366669068958 >5k 3.012697823769758E-4 1.2728636162108076 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8431 0.7221710185943405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6393 0.5476028136488695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4973 0.4259704039223882 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3954 0.3386863014496528 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1410 0.12077584346080182 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1320 0.11306674706968681 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1188 0.10176007236271811 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.2828313283972275E-5 0.0 14-15 0.0 0.0 0.0 1.713132531358891E-4 0.0 16-17 0.0 0.0 0.0 2.997981929878059E-4 0.0 18-19 0.0 0.0 0.0 4.7111144612369503E-4 0.0 20-21 0.0 0.0 0.0 7.70909639111501E-4 0.0 22-23 0.0 0.0 0.0 0.002655355423606281 0.0 24-25 0.0 0.0 0.0 0.007366469884843231 0.0 26-27 0.0 0.0 0.0 0.013747888564155101 0.0 28-29 0.0 0.0 0.0 0.02633941266964295 0.0 30-31 0.0 0.0 0.0 0.059531355464721464 0.0 32-33 0.0 0.0 0.0 0.12334554225784014 0.0 34-35 0.0 0.0 0.0 0.2247629881142865 0.0 36-37 0.0 0.0 0.0 0.3807865334077975 0.0 38-39 0.0 0.0 0.0 0.5835357684941223 0.0 40-41 0.0 0.0 0.0 0.7967779403350201 0.0 42-43 0.0 0.0 0.0 1.0026964706043588 0.0 44-45 0.0 0.0 0.0 1.2591524105487848 0.0 46-47 0.0 0.0 0.0 1.5700859649904237 0.0 48-49 0.0 0.0 0.0 1.8807197212390745 0.0 50-51 0.0 0.0 0.0 2.183944179289598 0.0 52-53 0.0 0.0 0.0 2.485070049989207 0.0 54-55 0.0 0.0 0.0 2.8205013996292783 0.0 56-57 0.0 0.0 0.0 3.2213315836539747 0.0 58-59 0.0 8.565662656794455E-5 0.0 3.631883794794133 0.0 60-61 0.0 8.565662656794455E-5 0.0 4.055027530039779 0.0 62-63 4.2828313283972275E-5 8.565662656794455E-5 0.0 4.472475099618657 0.0 64-65 8.565662656794455E-5 8.565662656794455E-5 0.0 4.928596636092961 0.0 66-67 8.565662656794455E-5 8.565662656794455E-5 0.0 5.4506309467113 0.0 68-69 8.565662656794455E-5 8.565662656794455E-5 0.0 6.002131136869011 0.0 70-71 8.565662656794455E-5 8.565662656794455E-5 0.0 6.589692766811826 0.0 72-73 8.565662656794455E-5 8.565662656794455E-5 0.0 7.174256414824764 0.0 74-75 8.565662656794455E-5 8.565662656794455E-5 0.0 7.781090785745366 0.0 76-77 8.565662656794455E-5 8.565662656794455E-5 0.0 8.442659741042887 0.0 78-79 8.565662656794455E-5 8.565662656794455E-5 0.0 9.165430356023203 0.0 80-81 8.565662656794455E-5 8.565662656794455E-5 0.0 9.91475452523958 0.0 82-83 8.565662656794455E-5 8.565662656794455E-5 0.0 10.651829796856745 0.0 84-85 8.565662656794455E-5 8.565662656794455E-5 0.0 11.388990725100475 0.0 86-87 8.565662656794455E-5 8.565662656794455E-5 0.0 12.175318556994206 0.0 88-89 8.565662656794455E-5 8.565662656794455E-5 0.0 12.997022575660498 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4855 0.0 34.22714 1 AGCATCG 30 0.003944438 31.678698 18-19 CATTCCG 180 5.456968E-12 31.669193 9 GTATCAA 7130 0.0 30.31801 1 GTACATG 5130 0.0 25.616873 1 TATCAAC 8575 0.0 25.052782 2 TCAACGC 8635 0.0 24.934814 4 CAACGCA 8705 0.0 24.734303 5 ATCAACG 8720 0.0 24.691757 3 TACATGG 5205 0.0 24.380474 2 AACGCAG 8945 0.0 24.171757 6 CATGGGT 1125 0.0 23.23702 4 GAGTACT 5635 0.0 23.138727 12-13 ACATGGG 5290 0.0 22.911568 3 ACGCAGA 9865 0.0 21.862793 7 CGCAGAG 9885 0.0 21.81856 8 CATGGGG 2080 0.0 21.251514 4 AGTACTT 5870 0.0 20.715382 12-13 GTACTTT 6285 0.0 20.52161 14-15 GCAGAGT 10240 0.0 20.504566 9 >>END_MODULE