FastQCFastQC Report
Thu 26 May 2016
SRR938414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167452
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78990.6766016932601939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63130.540750283523434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50450.4321376810352802No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40950.3507638857957329No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19710.1688292109654187No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.12000493382169032No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.11298109044311885No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCG500.001611250138.0219425
ACGCCGA550.00258101134.5417186
GGTATCA49350.031.6021881
GTATCAA70700.030.1965561
CGCCGAA700.00839605427.139927
CATTCCG2900.026.2040629
CATGGGG22750.024.6515884
GTACATG59550.024.4326271
TATCAAC89000.023.827332
TCAACGC89250.023.6438984
CAACGCA89600.023.5515385
ATCAACG90300.023.42163
TACATGG60550.023.3223762
AACGCAG91500.023.2024086
ACATGGG59300.023.0824623
GTATAGG4950.022.092921
GAGTACT55050.021.35327512-13
AGTACTT57000.020.95606612-13
CGCAGAG102200.020.7267198
ACGCAGA102500.020.7123937
Produced by FastQC (version 0.11.5)