##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938414_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1167452 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79428618906816 31.0 31.0 34.0 30.0 34.0 2 32.0222698663414 31.0 31.0 34.0 30.0 34.0 3 32.0775440874657 33.0 31.0 34.0 30.0 34.0 4 35.74857467373391 37.0 35.0 37.0 33.0 37.0 5 35.6288147178642 37.0 35.0 37.0 33.0 37.0 6 35.6547609666179 37.0 35.0 37.0 33.0 37.0 7 35.60108852441043 37.0 35.0 37.0 33.0 37.0 8 35.604507936943016 37.0 35.0 37.0 33.0 37.0 9 37.170530351569056 39.0 37.0 39.0 33.0 39.0 10-11 37.09451609145387 39.0 37.0 39.0 33.0 39.0 12-13 37.00974386955524 39.0 37.0 39.0 33.0 39.0 14-15 38.049442717987546 40.0 37.0 41.0 33.0 41.0 16-17 37.99598356078023 40.0 37.0 41.0 33.0 41.0 18-19 38.14265468730192 40.0 37.5 41.0 33.0 41.0 20-21 38.21517158735434 40.0 37.5 41.0 33.0 41.0 22-23 38.10216265850759 40.0 37.0 41.0 33.0 41.0 24-25 38.01648418949986 40.0 37.0 41.0 33.0 41.0 26-27 37.83142947204682 40.0 37.0 41.0 32.5 41.0 28-29 37.7106716164776 40.0 37.0 41.0 32.5 41.0 30-31 37.46898759006794 39.0 36.0 41.0 32.0 41.0 32-33 37.22919957308737 39.0 36.0 41.0 31.5 41.0 34-35 37.05007015277716 39.0 35.5 41.0 31.0 41.0 36-37 36.9957158838222 39.0 35.5 41.0 31.0 41.0 38-39 36.864028242702915 39.0 35.0 41.0 31.0 41.0 40-41 36.7048028527083 39.0 35.0 41.0 30.5 41.0 42-43 36.47562512206069 39.0 35.0 41.0 30.0 41.0 44-45 36.168912297893186 39.0 35.0 41.0 30.0 41.0 46-47 35.76477962263117 39.0 35.0 40.0 28.5 41.0 48-49 35.41677816304225 39.0 35.0 40.0 27.0 41.0 50-51 35.103674926249646 38.5 34.5 40.0 26.0 41.0 52-53 34.70238176815835 38.0 34.0 40.0 24.0 41.0 54-55 34.44482899511072 38.0 34.0 40.0 24.0 41.0 56-57 34.150157351223 38.0 33.0 40.0 23.0 41.0 58-59 33.768073548205834 38.0 33.0 40.0 22.0 41.0 60-61 33.45856489174716 37.5 33.0 40.0 21.0 41.0 62-63 33.05691197582428 37.0 32.0 39.5 19.5 41.0 64-65 32.61552038113773 36.5 31.0 39.0 17.5 40.0 66-67 32.1631506049071 36.0 31.0 39.0 13.5 40.0 68-69 31.660562061652215 35.5 30.5 38.5 10.0 40.0 70-71 31.14152230669869 35.0 30.0 38.0 8.0 40.0 72-73 30.73467645779013 35.0 29.0 37.5 7.0 39.5 74-75 30.259263335880192 34.0 29.0 37.0 4.5 39.0 76-77 28.876750393163917 32.5 27.5 35.5 2.0 38.0 78-79 29.59072235946317 34.0 29.0 36.0 2.0 38.5 80-81 29.310441457122007 34.0 29.0 36.0 2.0 37.5 82-83 28.86008718131452 34.0 28.5 35.0 2.0 37.0 84-85 28.36514991622782 34.0 27.5 35.0 2.0 36.5 86-87 27.842764413440555 33.0 26.5 35.0 2.0 36.0 88-89 27.252584688706687 33.0 26.0 35.0 2.0 35.5 90-91 26.76159962036983 32.0 25.5 35.0 2.0 35.0 92-93 26.12895562301491 32.0 24.5 34.0 2.0 35.0 94-95 25.455864566594602 32.0 21.5 34.0 2.0 35.0 96-97 24.576171868308077 31.0 17.5 34.0 2.0 35.0 98-99 23.47375009850512 31.0 2.0 34.0 2.0 35.0 100-101 19.818619523543582 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2680125107146303 1101 2 0.28446181661597336 1101 3 0.20639463121305823 1101 4 0.16617850334712614 1101 5 0.08955568358346255 1101 6 0.16523018587939475 1101 7 0.23809081547778277 1101 8 0.28091630132572476 1101 9 0.27771586407688886 1101 10-11 0.26486460568325754 1101 12-13 0.38012168643246724 1101 14-15 0.27109919851549336 1101 16-17 0.3441328291542476 1101 18-19 0.4245983387067156 1101 20-21 0.3446604542912226 1101 22-23 0.23807465050500554 1101 24-25 0.3907928107966683 1101 26-27 0.47594596621660656 1101 28-29 0.30343723917941645 1101 30-31 0.3103751394855365 1101 32-33 0.14398926232821907 1101 34-35 0.2681549937171894 1101 36-37 0.36206844964487317 1101 38-39 0.47763686615947876 1101 40-41 0.4659300209122179 1101 42-43 0.49051377607177216 1101 44-45 0.6134554041769107 1101 46-47 0.34654016326721404 1101 48-49 0.4816741625327339 1101 50-51 0.5539420474536669 1101 52-53 0.49424167860619406 1101 54-55 0.46276475643699655 1101 56-57 0.4771619669121918 1101 58-59 0.3034587047973005 1101 60-61 0.034606740884719045 1101 62-63 0.2317574884347593 1101 64-65 0.4021323282491025 1101 66-67 0.3238381815393083 1101 68-69 0.4168259992906336 1101 70-71 0.3126598487118102 1101 72-73 0.3706776753240497 1101 74-75 0.33828758846689766 1101 76-77 0.2960379702687952 1101 78-79 0.4547649775109761 1101 80-81 0.5193516354910308 1101 82-83 0.5429046196077039 1101 84-85 0.38540693598980624 1101 86-87 0.5580619462813985 1101 88-89 0.5516566768707527 1101 90-91 0.909655615056117 1101 92-93 0.7658942856930189 1101 94-95 0.7284351280266428 1101 96-97 1.0986587862694002 1101 98-99 0.9153602275442339 1101 100-101 0.9248992545765624 1102 1 0.18104669874881907 1102 2 0.14063465612215253 1102 3 0.09271941753785029 1102 4 0.15340547390743353 1102 5 0.19349680419124837 1102 6 0.1768161307986702 1102 7 0.1250803691340039 1102 8 0.11325248366190976 1102 9 0.25879373682143125 1102 10-11 0.28828578327109966 1102 12-13 0.2760974699082581 1102 14-15 0.41320508645471676 1102 16-17 0.2826774635322167 1102 18-19 0.24253518581022604 1102 20-21 0.2725094571402238 1102 22-23 0.32542032951734967 1102 24-25 0.41099698933418694 1102 26-27 0.18020759964490196 1102 28-29 0.11332565323449018 1102 30-31 0.11160970738676923 1102 32-33 0.2122822347878568 1102 34-35 0.026440843669711 1102 36-37 0.2647631315062213 1102 38-39 0.08989944925539106 1102 40-41 0.10155442736996179 1102 42-43 0.1438257418837381 1102 44-45 0.28386138708289366 1102 46-47 -0.06268348345643204 1102 48-49 0.07109723945581692 1102 50-51 -0.14696013678184983 1102 52-53 -0.052944931080411095 1102 54-55 -0.05077987794097538 1102 56-57 0.15071372379728132 1102 58-59 0.3431310694696563 1102 60-61 0.26905593366723934 1102 62-63 0.2486022177794851 1102 64-65 0.29660051438396096 1102 66-67 0.24767483870930107 1102 68-69 -0.09084256671127022 1102 70-71 0.019972289357585993 1102 72-73 0.11991794123098387 1102 74-75 0.16923488191418912 1102 76-77 0.15605696362112198 1102 78-79 0.11195870970470523 1102 80-81 0.0834779412840021 1102 82-83 0.14575362245670576 1102 84-85 -0.06356030047742678 1102 86-87 -0.1669499245638022 1102 88-89 0.2465047300521377 1102 90-91 -0.0999241570236542 1102 92-93 0.07009656489530158 1102 94-95 0.20786295328780469 1102 96-97 0.03447312625040766 1102 98-99 0.02093952479019734 1102 100-101 -0.670945949601979 1103 1 0.35292126848584005 1103 2 0.26671203461588533 1103 3 0.23091300145633653 1103 4 0.2487792863816125 1103 5 0.1984603178908273 1103 6 0.2728921324508846 1103 7 0.3062727138951189 1103 8 0.34549811219006443 1103 9 0.270658668667771 1103 10-11 0.3621997995178319 1103 12-13 0.4356613553550446 1103 14-15 0.7216674986810645 1103 16-17 0.5105051518152948 1103 18-19 0.512132803874394 1103 20-21 0.42399300388399297 1103 22-23 0.6453845316376885 1103 24-25 0.5575927486622803 1103 26-27 0.6254316806031852 1103 28-29 0.620631518579394 1103 30-31 0.5933390203428459 1103 32-33 0.5671063432829726 1103 34-35 0.6257661913221924 1103 36-37 0.6119801905727229 1103 38-39 0.5526266281070988 1103 40-41 0.6422486997092136 1103 42-43 0.7428292528296296 1103 44-45 0.9092658342810225 1103 46-47 0.7541157042070736 1103 48-49 0.9432867616918017 1103 50-51 0.6861563440112093 1103 52-53 0.8951911697043826 1103 54-55 0.8452875184517055 1103 56-57 0.8768255233291526 1103 58-59 0.8301681065066973 1103 60-61 0.8563993179437119 1103 62-63 0.8464629502591876 1103 64-65 0.7075877097577674 1103 66-67 0.7846017284242919 1103 68-69 0.6613018639647628 1103 70-71 0.5020320442797939 1103 72-73 0.4321064308358302 1103 74-75 0.7308330507149599 1103 76-77 0.6232571563789371 1103 78-79 0.6954262850867821 1103 80-81 0.5436321475348613 1103 82-83 0.49661759051398136 1103 84-85 0.37763307691483305 1103 86-87 0.5117038686345978 1103 88-89 0.3538226427603881 1103 90-91 0.5205959448626167 1103 92-93 0.777266467139551 1103 94-95 0.6816909169391856 1103 96-97 0.7581530794377969 1103 98-99 0.688804043584085 1103 100-101 0.3078231540741889 1104 1 0.3603758299908755 1104 2 0.2915630251243968 1104 3 0.29859257301522035 1104 4 0.1906819324239919 1104 5 0.1780125864642912 1104 6 0.15559068371570817 1104 7 0.26736513411006513 1104 8 0.24757770598185402 1104 9 0.5154560059507176 1104 10-11 0.4867698544134882 1104 12-13 0.3851844922868324 1104 14-15 0.40427442096072497 1104 16-17 0.4829571957710499 1104 18-19 0.5880802361375999 1104 20-21 0.5944556117380912 1104 22-23 0.5256084463941235 1104 24-25 0.46398487167562763 1104 26-27 0.6825199713833996 1104 28-29 0.5294243046563665 1104 30-31 0.7423436075138099 1104 32-33 0.6124422562869256 1104 34-35 0.6778651571652645 1104 36-37 0.7759731872083577 1104 38-39 0.8493578192348679 1104 40-41 0.7674820634493145 1104 42-43 0.7607819130945259 1104 44-45 0.44386456308210853 1104 46-47 0.3366287905590113 1104 48-49 0.3836241132953546 1104 50-51 0.2901111033236887 1104 52-53 0.36667175024471277 1104 54-55 0.36058653464575485 1104 56-57 0.4950653313415003 1104 58-59 0.49496581978726084 1104 60-61 0.1806776477295884 1104 62-63 0.23848910334579188 1104 64-65 0.6501258735161812 1104 66-67 0.3851418571789509 1104 68-69 0.1634401460806778 1104 70-71 0.07720868142247639 1104 72-73 0.11010166057037551 1104 74-75 0.28193521417257017 1104 76-77 0.30869059989592174 1104 78-79 0.3317842740553374 1104 80-81 0.5560641818781598 1104 82-83 0.5165344296491234 1104 84-85 0.47167008161970614 1104 86-87 0.5076994939840311 1104 88-89 0.8360996679980026 1104 90-91 0.7794208542144965 1104 92-93 0.9099944443050525 1104 94-95 0.7017467117164138 1104 96-97 0.7748559563100166 1104 98-99 0.6333039340543429 1104 100-101 0.5433533761089571 1105 1 0.25576179846391867 1105 2 0.2877856532731471 1105 3 0.265653890472322 1105 4 0.14784991835187355 1105 5 0.06343982413427085 1105 6 0.17425202823456942 1105 7 0.2176729617265991 1105 8 0.2062724266818492 1105 9 0.27439202741971513 1105 10-11 0.349859857345173 1105 12-13 0.29422425053503787 1105 14-15 0.4197221804718083 1105 16-17 0.32053358888833827 1105 18-19 0.29363917441422416 1105 20-21 0.2559140155447821 1105 22-23 0.2638106429076643 1105 24-25 0.4019514052885924 1105 26-27 0.2730494799867884 1105 28-29 0.2183470207558642 1105 30-31 0.44394588972295423 1105 32-33 0.5325457675513903 1105 34-35 0.3465975388264013 1105 36-37 0.4841007383438338 1105 38-39 0.38637380822974876 1105 40-41 0.2746669629825007 1105 42-43 0.3930778786358715 1105 44-45 0.3718599625814676 1105 46-47 0.06491623164328786 1105 48-49 -0.03655945570088193 1105 50-51 0.13466379484207636 1105 52-53 0.20976874413326385 1105 54-55 0.14704775738666598 1105 56-57 0.12450289758645283 1105 58-59 0.35260400394259506 1105 60-61 0.05616419577550147 1105 62-63 0.2095352662125407 1105 64-65 0.23228427506771965 1105 66-67 0.07716201819647495 1105 68-69 -0.04376279463149402 1105 70-71 0.18820590759120392 1105 72-73 0.22860739992044188 1105 74-75 0.262789012968323 1105 76-77 0.220017077581236 1105 78-79 0.26046298320897776 1105 80-81 0.5393896221956815 1105 82-83 0.49005561675869913 1105 84-85 0.4975151980980712 1105 86-87 0.5369318418179603 1105 88-89 0.22563578418319352 1105 90-91 0.32888638428688566 1105 92-93 0.49823046802920246 1105 94-95 0.49087577380062086 1105 96-97 0.7278135820908638 1105 98-99 0.3711532000038744 1105 100-101 0.04422499056896356 1106 1 0.08739898126389178 1106 2 0.08348564664755997 1106 3 0.18910184647841533 1106 4 0.06611003893758038 1106 5 0.011197062589182849 1106 6 -0.05522521287057458 1106 7 0.03033446043460941 1106 8 -0.06732460505471494 1106 9 0.029013375064323554 1106 10-11 0.09146192452863744 1106 12-13 -0.1085816859918296 1106 14-15 -0.05327854834287393 1106 16-17 -0.12915500847466888 1106 18-19 0.06239952689550421 1106 20-21 0.12098060270439248 1106 22-23 0.13887855518488124 1106 24-25 0.19465332008353897 1106 26-27 0.03314687613069367 1106 28-29 0.11930110543087835 1106 30-31 0.21419368671494254 1106 32-33 0.2633050599385882 1106 34-35 0.028960223514665984 1106 36-37 -0.28423557650411624 1106 38-39 -0.02496919846209522 1106 40-41 0.18720257551810704 1106 42-43 0.36890110794747244 1106 44-45 0.5567403707176837 1106 46-47 0.009463372702050776 1106 48-49 0.13568595607941347 1106 50-51 0.39554632316647087 1106 52-53 0.3552699698670452 1106 54-55 -0.022173073462077753 1106 56-57 0.04067927073957378 1106 58-59 0.34728696955578897 1106 60-61 0.021532247190073406 1106 62-63 -0.05310966852541554 1106 64-65 -0.44230990766599376 1106 66-67 0.30745089383418645 1106 68-69 -0.5992189663667382 1106 70-71 -0.2264532059051163 1106 72-73 -0.16444898383361561 1106 74-75 0.2510285942311583 1106 76-77 0.116694345188737 1106 78-79 0.039241224777921246 1106 80-81 -0.7980250733585486 1106 82-83 -0.2303015024356263 1106 84-85 -0.12625409892704198 1106 86-87 0.20745791350682197 1106 88-89 0.42912746907022736 1106 90-91 0.3175930695052003 1106 92-93 0.03863661657488748 1106 94-95 -0.09875849374062184 1106 96-97 -0.0794480623800844 1106 98-99 -0.17154540199705437 1106 100-101 -0.52820262488191 1107 1 0.11483379710545094 1107 2 0.15048871547951848 1107 3 0.1669897143246999 1107 4 0.10298236868300137 1107 5 0.026357976621959267 1107 6 0.11300082542707912 1107 7 0.15089591978399142 1107 8 0.17808569435604937 1107 9 0.3585177986315813 1107 10-11 0.24316375727158857 1107 12-13 0.17253480127936172 1107 14-15 0.28739566776449976 1107 16-17 0.32525779220526374 1107 18-19 0.22243155569004358 1107 20-21 0.21217710492045683 1107 22-23 0.2397353896105514 1107 24-25 0.3430456770947856 1107 26-27 0.3520159145823598 1107 28-29 0.2544220476421444 1107 30-31 0.14066293279761766 1107 32-33 0.34406724827816504 1107 34-35 -0.0376902576302669 1107 36-37 -0.057155558707172816 1107 38-39 -0.007469940001421094 1107 40-41 0.07822232042858701 1107 42-43 0.16088791995585439 1107 44-45 0.06537966032302478 1107 46-47 -0.09250933579883736 1107 48-49 -0.1025200036150693 1107 50-51 0.07169328485170468 1107 52-53 0.262682368404505 1107 54-55 0.15998784342277617 1107 56-57 0.16024892124638512 1107 58-59 0.001525722400735674 1107 60-61 -0.08144688703425373 1107 62-63 0.06603537941794713 1107 64-65 0.08334628333171423 1107 66-67 -0.2224962429684041 1107 68-69 -0.30552554439093527 1107 70-71 -0.21122099001450323 1107 72-73 -0.09181556379722622 1107 74-75 -0.004977161254807072 1107 76-77 -0.23851012001880534 1107 78-79 -0.04363363760949568 1107 80-81 -0.15868234814449877 1107 82-83 -0.1555279571361332 1107 84-85 0.056745684315309575 1107 86-87 0.19322606268171683 1107 88-89 0.025337204911103584 1107 90-91 0.30467174682721776 1107 92-93 0.012336569800869057 1107 94-95 0.1446639098735254 1107 96-97 -0.12177534075964758 1107 98-99 0.10545019440020553 1107 100-101 -0.5835931166365604 1108 1 0.0905271429491954 1108 2 0.1735134162661467 1108 3 0.12951849008651095 1108 4 0.10864069265171139 1108 5 0.13590634717740357 1108 6 0.08360727119772093 1108 7 0.0013083269105607087 1108 8 0.036624867577948805 1108 9 0.13426051331785516 1108 10-11 0.11381738983647693 1108 12-13 0.12132826495371063 1108 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-0.5653424558797084 2316 66-67 -0.46769033692881123 2316 68-69 -0.42129563160330363 2316 70-71 -0.6139198286891947 2316 72-73 -0.46123079658947574 2316 74-75 -0.15398943224075268 2316 76-77 -0.05728086245362718 2316 78-79 -0.21667198695100964 2316 80-81 -0.42194213003826775 2316 82-83 -0.16339753021226144 2316 84-85 -0.1815575907927638 2316 86-87 -0.13185213723895117 2316 88-89 -0.28757481955053166 2316 90-91 -0.3740772050679091 2316 92-93 -0.12449038369089038 2316 94-95 -0.0685764789420027 2316 96-97 -0.21276559275669626 2316 98-99 -0.2571520428957612 2316 100-101 -0.14226470960534598 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 24.0 5 50.0 6 119.0 7 298.0 8 612.0 9 1066.0 10 1738.0 11 2761.0 12 4011.0 13 5451.0 14 6960.0 15 9047.0 16 11489.0 17 14310.0 18 12936.0 19 9664.0 20 9010.0 21 9255.0 22 10032.0 23 11340.0 24 12910.0 25 15061.0 26 17698.0 27 20996.0 28 25501.0 29 30640.0 30 36770.0 31 43732.0 32 53300.0 33 65958.0 34 84695.0 35 113557.0 36 155360.0 37 219646.0 38 144256.0 39 7196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.79123606034727 22.515189178401002 15.758885016227772 21.934689745023956 2 14.668406916894766 22.922797677914307 40.7108002351566 21.697995170034332 3 17.58399989035858 27.118178033683897 32.265328812908905 23.032493263048615 4 11.514905966155354 19.818630658905036 39.67640639615162 28.990056978787994 5 11.488276394187492 39.79321874062326 36.029576921428266 12.688927943760984 6 27.74469528511665 40.288851276112425 18.582434224276458 13.384019214494472 7 25.2033488314723 35.18877007363044 22.556901697029087 17.050979397868176 8 24.039275276413935 36.82052880974978 21.301346864796155 17.83884904904013 9 24.96248239756324 17.496479512648058 22.44494848610478 35.09608960368392 10-11 22.60289930549607 28.7236220418484 29.51174866290006 19.161729989755468 12-13 23.836269071447905 27.02697841110384 29.095414629466564 20.04133788798169 14-15 20.92672760850125 28.766792981638645 27.890654176788427 22.415825233071683 16-17 19.0445945529238 31.698433854239834 29.10059685537393 20.15637473746244 18-19 18.756488489462523 30.08975957898055 32.112797785262266 19.040954146294666 20-21 19.995597249394407 29.185225602423053 31.860838818212656 18.958338329969884 22-23 19.48812456529262 29.676466355790215 31.389427573895972 19.445981505021194 24-25 19.15963960815503 29.911649434687437 31.943511143717746 18.985199813439788 26-27 19.168454912643647 29.87360206280063 31.9132732825343 19.044669742021426 28-29 18.589201097775327 29.893091964380545 32.10864343887372 19.409063498970408 30-31 19.308888074199196 29.60451478947314 32.14701760757615 18.939579528751505 32-33 18.96900257997759 29.755484593799146 31.926880077296538 19.348632748926722 34-35 19.261202305632025 30.009538980678318 31.861351965257036 18.867906748432624 36-37 19.357360058246606 29.452010792753434 31.546790012420235 19.643839136579725 38-39 18.943509200982522 29.944899038677747 31.711513381203144 19.400078379136588 40-41 19.320590569578155 29.516057946780894 32.0395688984278 19.123782585213146 42-43 19.172107318025844 29.45785072714284 31.72801996837541 19.642021986455905 44-45 19.31064403504384 29.53727433761731 31.433155281758907 19.718926345579945 46-47 19.50298599000216 29.44446538273094 30.92773835669475 20.12481027057215 48-49 19.27162744164214 29.365918256168133 31.122692838763395 20.239761463426333 50-51 19.145712200587262 29.626485714187822 30.868078516290176 20.35972356893474 52-53 19.20156032110956 29.969155048772883 30.823794040354553 20.005490589763006 54-55 19.50178679723021 29.603015798508203 30.547979702805765 20.34721770145582 56-57 19.611084652730902 29.803152506484203 30.1647091272275 20.421053713557388 58-59 19.34118062241531 29.620147123821795 30.455213576232687 20.58345867753021 60-61 19.50881064060878 29.76987490706256 30.269895464652937 20.451418987675723 62-63 19.211111034971886 29.617406111771622 30.50348108530372 20.668001767952774 64-65 19.183829399409998 29.59432165091156 30.237988371256375 20.983860578422068 66-67 19.11735985719327 29.625543491295574 29.906668539691566 21.350428111819586 68-69 19.194915036464337 29.335675776612458 30.141454564766384 21.327954622156817 70-71 19.213338107262654 29.1525047710741 30.222741491727284 21.411415629935963 72-73 19.261006019947715 28.705077382196443 30.23982998872759 21.794086609128254 74-75 19.01962564627925 29.059995614380718 29.799811898048056 22.120566841291975 76-77 18.8006444804583 28.802554623230765 30.36878604002563 22.02801485628531 78-79 18.650359929144837 28.630384803829195 30.17841418747837 22.540841079547597 80-81 18.63473399601091 28.64179648468802 30.07262499726966 22.65084452203141 82-83 18.655525527969196 28.662592242032815 30.145916938271185 22.535965291726807 84-85 18.559010786860462 28.265686133841015 30.085211272129982 23.090091807168537 86-87 18.283630831669065 28.464545396531438 30.088669165889677 23.16315460590982 88-89 18.660346918051843 28.099454238570083 29.93601875538299 23.304180087995093 90-91 18.46164388842014 27.87145102641306 30.26464453514817 23.40226055001863 92-93 18.356854804170478 27.879367233032166 30.19468171492594 23.569096247871418 94-95 18.144728862514263 27.60576023682344 30.287026789966525 23.962484110695772 96-97 18.34794920904671 27.358169757728795 30.09922463621631 24.194656397008185 98-99 18.215197040389704 27.286841160785336 29.931406114689178 24.56655568413578 100-101 17.91645395271069 27.137775257569473 30.37551008521121 24.570260704508623 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2395.0 1 1462.5 2 635.5 3 761.5 4 666.5 5 704.0 6 1235.5 7 2206.0 8 2994.5 9 3005.0 10 2339.0 11 1668.0 12 1515.0 13 1500.0 14 1454.5 15 1462.0 16 1512.0 17 1689.5 18 1877.0 19 2050.0 20 2319.0 21 2661.0 22 3109.5 23 3640.0 24 4304.0 25 5664.5 26 7960.0 27 10595.0 28 12467.5 29 14787.5 30 18621.0 31 22680.0 32 27335.0 33 32861.0 34 39256.0 35 45056.0 36 49740.5 37 53607.0 38 53608.0 39 51821.0 40 50875.5 41 49703.5 42 48530.0 43 47959.5 44 47991.0 45 47546.5 46 45721.0 47 43713.5 48 43310.5 49 41273.5 50 36941.5 51 32215.0 52 27163.5 53 22692.0 54 19317.5 55 16209.5 56 13187.0 57 10691.5 58 8231.5 59 6059.0 60 4370.0 61 3092.0 62 2089.0 63 1348.5 64 828.0 65 525.0 66 391.5 67 322.0 68 222.5 69 152.5 70 127.0 71 112.0 72 107.5 73 112.5 74 110.5 75 84.5 76 42.5 77 18.5 78 12.0 79 7.5 80 4.0 81 4.0 82 4.5 83 1.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.70909639111501E-4 2 0.04882427714372839 3 8.565662656794455E-4 4 0.0 5 0.08582793982108043 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.2828313283972275E-5 26-27 0.009165259042770067 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011692129526524432 36-37 1.713132531358891E-4 38-39 0.004240003015113255 40-41 0.006038792173040091 42-43 5.139397594076673E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.565662656794455E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001413334338371085 82-83 5.995963859756118E-4 84-85 0.0050965692807927 86-87 0.0019272740977787524 88-89 4.2828313283972275E-5 90-91 0.003169295183013948 92-93 6.852530125435564E-4 94-95 0.0 96-97 0.0 98-99 8.565662656794455E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1167452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.223698627523824 #Duplication Level Percentage of deduplicated Percentage of total 1 76.26726762523893 42.11760602480899 2 13.903921125596899 15.356518999616501 3 4.544777236746075 7.529382253538872 4 2.0313894166479214 4.487233477604249 5 0.9926291901825972 2.740832762376339 6 0.5335911550521637 1.7680126282147817 7 0.33000458320268017 1.2756851553940494 8 0.21386431754113633 0.9448302895258217 9 0.16263745138004884 0.8083297440504338 >10 0.8481295086501481 9.014481217105821 >50 0.1000323618971235 3.83911241301565 >100 0.0678815160185982 6.646352153087889 >500 0.0024796875812428485 0.8848731349626111 >1k 9.298828429660681E-4 0.9386211114358213 >5k 4.6494142148303404E-4 1.6481286352621145 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7899 0.6766016932601939 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6313 0.540750283523434 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5045 0.4321376810352802 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4095 0.3507638857957329 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1971 0.1688292109654187 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1401 0.12000493382169032 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1319 0.11298109044311885 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.2828313283972275E-5 0.0 14-15 0.0 0.0 0.0 1.713132531358891E-4 0.0 16-17 0.0 0.0 0.0 4.2828313283972273E-4 0.0 18-19 0.0 0.0 0.0 6.852530125435564E-4 0.0 20-21 0.0 0.0 0.0 0.0011991927719512238 0.0 22-23 0.0 0.0 0.0 0.0031692951830139485 0.0 24-25 0.0 0.0 0.0 0.00792323795753487 0.0 26-27 0.0 0.0 0.0 0.01434748495013071 0.0 28-29 0.0 0.0 0.0 0.026810524115766645 0.0 30-31 0.0 0.0 0.0 0.060345093417116934 0.0 32-33 0.0 0.0 0.0 0.12475887659621124 0.0 34-35 0.0 0.0 0.0 0.2269472320917691 0.0 36-37 0.0 0.0 0.0 0.3829279490719961 0.0 38-39 0.0 0.0 0.0 0.5862767805442965 0.0 40-41 0.0 0.0 0.0 0.800161377084454 0.0 42-43 0.0 0.0 0.0 1.006979301932756 0.0 44-45 0.0 0.0 0.0 1.264891404528837 0.0 46-47 0.0 0.0 0.0 1.5767671818627234 0.0 48-49 0.0 0.0 0.0 1.8870154832918185 0.0 50-51 0.0 0.0 0.0 2.1899829714626384 0.0 52-53 0.0 0.0 0.0 2.493036116260026 0.0 54-55 0.0 0.0 0.0 2.8308658514439995 0.0 56-57 0.0 0.0 0.0 3.2328095716140792 0.0 58-59 0.0 0.0 0.0 3.6449464303457444 0.0 60-61 0.0 0.0 0.0 4.06937501498991 0.0 62-63 0.0 0.0 0.0 4.486437129749232 0.0 64-65 0.0 8.565662656794455E-5 0.0 4.941530786704721 0.0 66-67 0.0 8.565662656794455E-5 0.0 5.462237419611256 0.0 68-69 0.0 8.565662656794455E-5 0.0 6.013138013382991 0.0 70-71 0.0 8.565662656794455E-5 0.0 6.603569140315834 0.0 72-73 0.0 8.565662656794455E-5 0.0 7.190017234113265 0.0 74-75 0.0 8.565662656794455E-5 0.0 7.8024621140740695 0.0 76-77 0.0 8.565662656794455E-5 0.0 8.472039963955691 0.0 78-79 0.0 8.565662656794455E-5 0.0 9.202905130146679 0.0 80-81 0.0 8.565662656794455E-5 0.0 9.960409507200296 0.0 82-83 0.0 8.565662656794455E-5 0.0 10.707934887258748 0.0 84-85 0.0 8.565662656794455E-5 0.0 11.457002086595423 0.0 86-87 0.0 8.565662656794455E-5 0.0 12.256178412474345 0.0 88-89 0.0 8.565662656794455E-5 0.0 13.094456988381534 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCCG 50 0.0016112501 38.021942 5 ACGCCGA 55 0.002581011 34.541718 6 GGTATCA 4935 0.0 31.602188 1 GTATCAA 7070 0.0 30.196556 1 CGCCGAA 70 0.008396054 27.13992 7 CATTCCG 290 0.0 26.204062 9 CATGGGG 2275 0.0 24.651588 4 GTACATG 5955 0.0 24.432627 1 TATCAAC 8900 0.0 23.82733 2 TCAACGC 8925 0.0 23.643898 4 CAACGCA 8960 0.0 23.551538 5 ATCAACG 9030 0.0 23.4216 3 TACATGG 6055 0.0 23.322376 2 AACGCAG 9150 0.0 23.202408 6 ACATGGG 5930 0.0 23.082462 3 GTATAGG 495 0.0 22.09292 1 GAGTACT 5505 0.0 21.353275 12-13 AGTACTT 5700 0.0 20.956066 12-13 CGCAGAG 10220 0.0 20.726719 8 ACGCAGA 10250 0.0 20.712393 7 >>END_MODULE