FastQCFastQC Report
Thu 26 May 2016
SRR938413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161205
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85210.7338066921861343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63330.5453817370748489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49000.42197544791832625No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38990.3357718921292967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14280.12297570196476935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.11281384423938924No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12440.10713009330824445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44700.033.57171
GTATCAA68000.031.1760671
TCAACGC81500.025.8964164
CAACGCA81950.025.7542175
ATCAACG82850.025.4744473
TATCAAC84000.025.0691012
AACGCAG84400.025.0586136
GTACATG50200.023.9148181
CATTCCG2853.6379788E-1223.3354829
TACATGG50650.022.9932822
GCACCGT1050.00218902122.6318556
CGCAGAG94300.022.3678158
ACGCAGA94800.022.2498427
CGCCGAC550.002883181721.58452892-93
ACATGGG51100.021.5814913
GAGTACT54750.021.42796512-13
AGTACTT55950.021.26550712-13
GCAGAGT98350.020.9144829
CATGGGT11900.019.9727294
GTACTTT58900.019.88043414-15
Produced by FastQC (version 0.11.5)