##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938413_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1161205 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.279637962289172 31.0 31.0 34.0 30.0 34.0 2 31.428576349567905 31.0 31.0 34.0 28.0 34.0 3 31.478618331819103 33.0 31.0 34.0 28.0 34.0 4 35.13789554815902 37.0 35.0 37.0 32.0 37.0 5 35.1641553386353 37.0 35.0 37.0 33.0 37.0 6 35.18906394650385 37.0 35.0 37.0 32.0 37.0 7 35.12794381698322 37.0 35.0 37.0 32.0 37.0 8 35.100328538027306 37.0 35.0 37.0 32.0 37.0 9 36.62863318707722 39.0 37.0 39.0 32.0 39.0 10-11 36.51700345761515 39.0 37.0 39.0 32.0 39.0 12-13 36.399384690902984 39.0 37.0 39.0 32.0 39.0 14-15 37.43176527831003 40.0 37.0 41.0 32.0 41.0 16-17 37.30647000314329 39.0 37.0 41.0 32.0 41.0 18-19 37.27302844889576 39.5 37.0 41.0 32.0 41.0 20-21 37.296688784495416 39.5 36.5 41.0 31.5 41.0 22-23 37.34772671492114 40.0 37.0 41.0 32.0 41.0 24-25 37.293116202565436 40.0 37.0 41.0 32.0 41.0 26-27 37.13810395235983 39.0 36.0 41.0 31.0 41.0 28-29 36.98147054137728 39.0 36.0 41.0 31.0 41.0 30-31 36.73431521565959 39.0 36.0 41.0 30.5 41.0 32-33 36.44874505362964 39.0 35.0 40.0 30.0 41.0 34-35 36.22416670613716 38.5 35.0 40.0 30.0 41.0 36-37 35.97985842293135 38.0 35.0 40.0 29.5 41.0 38-39 35.709081514461275 38.0 35.0 40.0 29.0 41.0 40-41 35.60281388729811 38.0 35.0 40.0 27.5 41.0 42-43 35.40477392019497 38.0 35.0 40.0 27.0 41.0 44-45 35.087278301419644 38.0 34.5 40.0 25.5 41.0 46-47 34.68839739753102 38.0 34.0 40.0 24.0 41.0 48-49 34.34492359230282 38.0 33.5 40.0 23.0 41.0 50-51 33.62295201966922 37.5 33.0 39.5 22.5 40.5 52-53 33.700224335926904 38.0 33.0 39.5 22.0 40.5 54-55 33.86641549080481 38.0 33.0 40.0 21.0 41.0 56-57 33.62783703135966 38.0 33.0 40.0 18.5 41.0 58-59 33.30630896353357 38.0 33.0 40.0 16.0 41.0 60-61 32.9430867073428 37.5 32.0 40.0 12.5 41.0 62-63 32.46775633931993 37.0 31.0 40.0 8.0 41.0 64-65 32.0827954581663 36.5 31.0 39.0 7.5 41.0 66-67 31.61022257051942 36.0 31.0 39.0 2.0 40.0 68-69 31.058039708750826 35.5 29.5 39.0 2.0 40.0 70-71 30.506898867986273 35.0 29.0 38.0 2.0 40.0 72-73 29.912633428206043 35.0 28.5 37.5 2.0 39.5 74-75 29.249258313562205 34.0 28.0 37.0 2.0 39.0 76-77 28.538622809925897 34.0 26.5 36.0 2.0 39.0 78-79 27.783938236573213 33.5 26.0 36.0 2.0 38.5 80-81 27.045333511309373 33.0 25.0 35.0 2.0 37.0 82-83 26.345613392984014 32.5 23.0 35.0 2.0 37.0 84-85 25.595274736157698 32.0 20.5 35.0 2.0 36.0 86-87 24.870556878415094 31.0 17.5 35.0 2.0 36.0 88-89 24.09020155786446 31.0 7.5 34.0 2.0 35.0 90-91 23.261294086746094 30.5 2.0 34.0 2.0 35.0 92-93 22.413442932126543 30.0 2.0 34.0 2.0 35.0 94-95 21.517387541390192 29.0 2.0 34.0 2.0 35.0 96-97 20.421871245817922 29.0 2.0 34.0 2.0 35.0 98-99 19.15996443349796 26.5 2.0 34.0 2.0 35.0 100-101 16.135627214832866 13.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.601246555347668 1101 2 0.5619614421978305 1101 3 0.5270153554947932 1101 4 0.43155360585258506 1101 5 0.47894425262319373 1101 6 0.3865743943940103 1101 7 0.3721504281552299 1101 8 0.39816213785444177 1101 9 0.5406078221062387 1101 10-11 0.710603952792404 1101 12-13 0.6495824794083092 1101 14-15 0.8099882603840172 1101 16-17 0.871630958492652 1101 18-19 0.8019629888936066 1101 20-21 0.750710891134041 1101 22-23 0.8284347488475206 1101 24-25 0.9818791550465207 1101 26-27 0.8698764880859713 1101 28-29 0.9558369379096447 1101 30-31 0.8557780513348376 1101 32-33 0.9117286394716828 1101 34-35 1.0830508489258577 1101 36-37 1.3318113904769788 1101 38-39 1.2194783361825898 1101 40-41 1.3180672567312612 1101 42-43 1.3803604523879471 1101 44-45 1.4060572933806768 1101 46-47 1.3148833780728353 1101 48-49 1.2907743544213943 1101 50-51 1.3572818337040573 1101 52-53 1.3647288301932363 1101 54-55 1.6159056470549231 1101 56-57 1.4363438278121734 1101 58-59 1.5866957475532146 1101 60-61 1.5388804024044944 1101 62-63 1.2429326448240943 1101 64-65 1.4336881920727294 1101 66-67 1.5480440288964274 1101 68-69 1.7950531503474387 1101 70-71 1.8185629917460062 1101 72-73 1.7924841823725224 1101 74-75 1.840724894471382 1101 76-77 2.2321942239623205 1101 78-79 2.709122298764722 1101 80-81 2.8894244621535954 1101 82-83 3.053578747313427 1101 84-85 3.032389478313341 1101 86-87 3.179746363449226 1101 88-89 3.370930364490782 1101 90-91 3.771878954035902 1101 92-93 3.6527488533807286 1101 94-95 3.991807041743826 1101 96-97 4.258004066070075 1101 98-99 4.5045435451276745 1101 100-101 4.620056767983218 1102 1 0.7697754933160574 1102 2 0.7383161220007857 1102 3 0.6219421642637322 1102 4 0.6865672635464222 1102 5 0.629846594275655 1102 6 0.6216968467088151 1102 7 0.8103732937028241 1102 8 0.6033217110806959 1102 9 0.879159873094963 1102 10-11 0.8370298393751767 1102 12-13 0.8130639072793429 1102 14-15 1.1671147749872475 1102 16-17 1.2539413266667196 1102 18-19 1.14244812885579 1102 20-21 0.9200289403017408 1102 22-23 0.8200603646110736 1102 24-25 0.9669630856607654 1102 26-27 0.9571339764710984 1102 28-29 0.7575435659767891 1102 30-31 0.7376821906666606 1102 32-33 0.7023176712929384 1102 34-35 0.7485120587407224 1102 36-37 0.9356495526416708 1102 38-39 0.8769970717369517 1102 40-41 1.0828747649608914 1102 42-43 1.1177335058747389 1102 44-45 1.1853816460993798 1102 46-47 1.3238787788152564 1102 48-49 1.1387793972621978 1102 50-51 1.2181554591797337 1102 52-53 1.2626673355512494 1102 54-55 1.5164216043775482 1102 56-57 1.5492871191996542 1102 58-59 1.4323054410138596 1102 60-61 1.3938917254498122 1102 62-63 1.6179449017288192 1102 64-65 1.616551871945724 1102 66-67 1.8055043982378294 1102 68-69 1.7907457238304936 1102 70-71 1.7243762665574849 1102 72-73 1.7496994136194637 1102 74-75 1.667776466857159 1102 76-77 1.8043885601050142 1102 78-79 2.266071380080529 1102 80-81 2.6280745683801037 1102 82-83 2.953501703912309 1102 84-85 2.9613484585388683 1102 86-87 3.175651389947486 1102 88-89 3.329308250700013 1102 90-91 3.4287649998417074 1102 92-93 3.586475185881369 1102 94-95 4.003200126514912 1102 96-97 4.244171273596979 1102 98-99 4.709371598264276 1102 100-101 4.949309913922097 1103 1 0.44212645709740883 1103 2 0.5539554964258038 1103 3 0.5184284280359286 1103 4 0.4682333997445767 1103 5 0.29197419447970674 1103 6 0.4427818800847305 1103 7 0.47517271120479876 1103 8 0.44894229384032514 1103 9 0.665824330687272 1103 10-11 0.4998245219006705 1103 12-13 0.7348182029842931 1103 14-15 0.9238642964755783 1103 16-17 0.8104602033524131 1103 18-19 0.87407088290297 1103 20-21 0.9636225520075143 1103 22-23 0.7456353416119939 1103 24-25 0.8314416060460132 1103 26-27 0.8745701131488559 1103 28-29 0.915212178663559 1103 30-31 0.8283962303515153 1103 32-33 0.9266583279984317 1103 34-35 1.0584978014911073 1103 36-37 0.992242549585292 1103 38-39 1.1869374703295392 1103 40-41 1.1509738419456852 1103 42-43 1.2367279119752794 1103 44-45 0.9559532876886934 1103 46-47 1.1018035859935864 1103 48-49 1.1067463657872452 1103 50-51 1.2979433008414247 1103 52-53 1.2210169826080204 1103 54-55 1.2913240490380602 1103 56-57 1.2565639845231473 1103 58-59 1.3693202258253123 1103 60-61 1.4943233669519245 1103 62-63 1.3635842325536913 1103 64-65 1.4494730385113783 1103 66-67 1.5044536345811679 1103 68-69 1.7088711789070778 1103 70-71 1.77637293550589 1103 72-73 1.8308022358572806 1103 74-75 2.08835225046089 1103 76-77 2.313700244509679 1103 78-79 2.21624861469731 1103 80-81 2.495953028597146 1103 82-83 2.4577679787589446 1103 84-85 2.673965470446067 1103 86-87 2.7489493490243824 1103 88-89 3.2222271500577833 1103 90-91 3.3600359006644425 1103 92-93 3.402561461861609 1103 94-95 3.7628608301341586 1103 96-97 4.139633805806593 1103 98-99 4.2964188651887625 1103 100-101 4.616332503160709 1104 1 0.531018722835352 1104 2 0.49456612200078354 1104 3 0.48695993677558036 1104 4 0.45843168467032314 1104 5 0.34202922799265423 1104 6 0.5495362170541114 1104 7 0.492778506972968 1104 8 0.3860315959825229 1104 9 0.6065253097909675 1104 10-11 0.6616045685559513 1104 12-13 0.6600658537925455 1104 14-15 0.8171465116155474 1104 16-17 0.8086383476552115 1104 18-19 0.9152942696817945 1104 20-21 0.7173672950749292 1104 22-23 0.7923817931824928 1104 24-25 0.9148071107115427 1104 26-27 0.8931677442436126 1104 28-29 0.7213849674662924 1104 30-31 0.8218032976402583 1104 32-33 0.8728893537573938 1104 34-35 0.8560188292214264 1104 36-37 0.9540250604277176 1104 38-39 1.0615349864288959 1104 40-41 1.2072760005736285 1104 42-43 1.1566997381022333 1104 44-45 1.2406478106220575 1104 46-47 1.1257977869398346 1104 48-49 1.1644966450617886 1104 50-51 1.1601451341966609 1104 52-53 1.3179461947252449 1104 54-55 1.4330854500105943 1104 56-57 1.4429017095855698 1104 58-59 1.5088478411492687 1104 60-61 1.7795361905818368 1104 62-63 1.7477202064004516 1104 64-65 1.8285927487229756 1104 66-67 1.7028469845614609 1104 68-69 2.0189447759632024 1104 70-71 2.1821559720415706 1104 72-73 2.2176073350818832 1104 74-75 2.238138687574832 1104 76-77 2.289444839725867 1104 78-79 2.5709757470405847 1104 80-81 2.7757852995920196 1104 82-83 2.951100299037563 1104 84-85 3.3127004389044714 1104 86-87 3.6199557230551385 1104 88-89 3.7606471132592567 1104 90-91 3.8468019835925524 1104 92-93 3.9297808730851607 1104 94-95 4.503906795438407 1104 96-97 5.186914164592242 1104 98-99 5.331437018034077 1104 100-101 5.284912063549722 1105 1 0.4837517842377679 1105 2 0.3726666015125524 1105 3 0.5391231887372179 1105 4 0.3896443201168296 1105 5 0.3660990062963023 1105 6 0.374960460421903 1105 7 0.36733168918743786 1105 8 0.4015301014003825 1105 9 0.4602843395580649 1105 10-11 0.42459848124743615 1105 12-13 0.5038637271267135 1105 14-15 0.7924956366622524 1105 16-17 0.741520711585963 1105 18-19 0.7328252511240549 1105 20-21 0.761940872108184 1105 22-23 0.6701911405482974 1105 24-25 0.6934046166276033 1105 26-27 0.6727857695269606 1105 28-29 0.6858209183941781 1105 30-31 0.6250450618699261 1105 32-33 0.6785862805228717 1105 34-35 0.7610396910971318 1105 36-37 0.8030080439873188 1105 38-39 0.8824264424009982 1105 40-41 0.9577162017194709 1105 42-43 0.9305036831763402 1105 44-45 1.0900309062753166 1105 46-47 1.0597547709976212 1105 48-49 0.9376179086064411 1105 50-51 0.5864287622948083 1105 52-53 0.7538072141548113 1105 54-55 0.7359983283266942 1105 56-57 0.92157666910731 1105 58-59 1.0560855886894345 1105 60-61 1.2846275002094387 1105 62-63 1.4598944341996827 1105 64-65 1.6172452116697897 1105 66-67 1.718094836099624 1105 68-69 1.8977154821532558 1105 70-71 1.7539064235299264 1105 72-73 1.9641884784372188 1105 74-75 2.162198346311907 1105 76-77 2.1145783401617884 1105 78-79 2.615066698591214 1105 80-81 2.8346819554644824 1105 82-83 2.8351670414712444 1105 84-85 3.087071858134763 1105 86-87 3.3011442272275104 1105 88-89 3.3818219170953476 1105 90-91 3.6422789265493343 1105 92-93 3.8109164441400907 1105 94-95 4.038594334359814 1105 96-97 4.194962333729119 1105 98-99 4.380662871191383 1105 100-101 4.416259756288543 1106 1 0.4792252174750935 1106 2 0.4379670818611672 1106 3 0.30618986696755 1106 4 0.48349868791140693 1106 5 0.5018381908448077 1106 6 0.3065590916489782 1106 7 0.4585507582818664 1106 8 0.46570312427970606 1106 9 0.5642462646650657 1106 10-11 0.5388912489349096 1106 12-13 0.4950875129797794 1106 14-15 0.7128824565170646 1106 16-17 0.4628112471815129 1106 18-19 0.5631486077520975 1106 20-21 0.5508548916842599 1106 22-23 0.5611598410508591 1106 24-25 0.7069451373881819 1106 26-27 0.8768555714687452 1106 28-29 0.7034452392189721 1106 30-31 0.7383819251645605 1106 32-33 0.9370091181553022 1106 34-35 0.8007551792588217 1106 36-37 0.9184704917416084 1106 38-39 0.7145672726418155 1106 40-41 0.8360694747695874 1106 42-43 0.5970520504931542 1106 44-45 0.7001786321129586 1106 46-47 1.3963997253591813 1106 48-49 1.4374920015813615 1106 50-51 0.8781378417508918 1106 52-53 1.2202391079638417 1106 54-55 1.1113857491878534 1106 56-57 1.099276366778291 1106 58-59 0.4639969623160667 1106 60-61 1.757845059941097 1106 62-63 1.869592089970638 1106 64-65 1.8443704537840588 1106 66-67 1.748988844446771 1106 68-69 1.3017545490871036 1106 70-71 1.6111115005880663 1106 72-73 1.9074477738752016 1106 74-75 2.2167537461637146 1106 76-77 2.349125249848033 1106 78-79 2.608994632605281 1106 80-81 2.9324466683279944 1106 82-83 2.835905366352449 1106 84-85 2.8308248156185094 1106 86-87 3.2831288718958227 1106 88-89 3.2510703244285395 1106 90-91 3.5459395116404195 1106 92-93 3.9486944856506057 1106 94-95 4.072840664797667 1106 96-97 4.461379696878456 1106 98-99 4.710242957949312 1106 100-101 4.523327514808759 1107 1 0.4018061349645059 1107 2 0.34065140140028305 1107 3 0.4765397949907566 1107 4 0.40551630876182543 1107 5 0.4578675754250341 1107 6 0.3575912927123994 1107 7 0.4274428105592918 1107 8 0.41387473907796135 1107 9 0.4024869742732733 1107 10-11 0.48261798747648044 1107 12-13 0.5714134797904009 1107 14-15 0.693086655187571 1107 16-17 0.8131257180829508 1107 18-19 0.8498982551458667 1107 20-21 0.7290265647043839 1107 22-23 0.7209777958037265 1107 24-25 0.7464432211607317 1107 26-27 0.644906172100157 1107 28-29 0.6805927894608104 1107 30-31 0.8227345051941697 1107 32-33 0.8379409592619851 1107 34-35 0.7520944279346438 1107 36-37 0.9909661869617352 1107 38-39 1.018843159906794 1107 40-41 0.9545233346615518 1107 42-43 1.1915960058253319 1107 44-45 1.195599481657446 1107 46-47 1.2884068808276012 1107 48-49 1.436786425235745 1107 50-51 1.3532949316469214 1107 52-53 1.3421229640568342 1107 54-55 1.42441602310722 1107 56-57 1.638440402543992 1107 58-59 1.746865757865585 1107 60-61 1.705827817532871 1107 62-63 1.6507178681423795 1107 64-65 1.6031939789147955 1107 66-67 1.9503419208178627 1107 68-69 2.010312582468618 1107 70-71 1.9256620932139796 1107 72-73 2.255507966560497 1107 74-75 2.401092935162108 1107 76-77 2.642001657907688 1107 78-79 2.681660990696013 1107 80-81 2.846779729474065 1107 82-83 2.99238284103685 1107 84-85 3.1577755759234165 1107 86-87 3.4188014873825523 1107 88-89 3.722730099080774 1107 90-91 4.101877269545131 1107 92-93 4.215028996521358 1107 94-95 4.143842605041648 1107 96-97 4.500764247995079 1107 98-99 4.90665408880734 1107 100-101 4.91218814182924 1108 1 0.3897943578854566 1108 2 0.23325902575282242 1108 3 0.3110525142633449 1108 4 0.33009184547199766 1108 5 0.2779984076710491 1108 6 0.3722276060077334 1108 7 0.18427524433022313 1108 8 0.283389437977398 1108 9 0.41045650298602965 1108 10-11 0.4173542481178245 1108 12-13 0.4251898050859495 1108 14-15 0.23556223638832563 1108 16-17 0.6397121066857423 1108 18-19 0.6011003172231568 1108 20-21 0.5967354493513213 1108 22-23 0.6424910334528349 1108 24-25 0.42392867328437234 1108 26-27 0.5865252075388767 1108 28-29 0.6314977034252749 1108 30-31 0.5599230541072728 1108 32-33 0.5994238154213463 1108 34-35 0.7001897683964486 1108 36-37 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1.6534096425052809 2316 100-101 1.796714794323126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5497.0 3 1124.0 4 953.0 5 953.0 6 1072.0 7 1324.0 8 1624.0 9 2065.0 10 2896.0 11 4029.0 12 5327.0 13 7221.0 14 8530.0 15 11279.0 16 13176.0 17 16046.0 18 14115.0 19 11540.0 20 11872.0 21 13047.0 22 14217.0 23 15970.0 24 18218.0 25 20691.0 26 23990.0 27 27818.0 28 32358.0 29 37399.0 30 43433.0 31 50813.0 32 59633.0 33 71433.0 34 87624.0 35 109532.0 36 131986.0 37 160102.0 38 112525.0 39 9773.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.89595325791762 21.810150025223034 15.361173112937 21.932723603922355 2 14.553222136742344 22.356941573976517 41.42166343460833 21.668172854672815 3 17.631622024450554 26.92645018996993 31.950874895538078 23.491052890041438 4 11.39708479728158 19.141636669728108 40.19914291179091 29.26213562119941 5 11.705674379166856 39.40535822152061 36.0090547412014 12.879912658111136 6 27.795308021996775 39.87397180952906 19.022967013461045 13.307753155013124 7 25.310763664556145 34.972993926863936 22.951199552052376 16.765042856527547 8 24.009779445590205 36.349265421665365 21.60049689783443 18.040458234910002 9 25.411416451144355 17.384337314106617 22.731932739806986 34.47231349494205 10-11 22.615877167252236 28.50480754942259 29.689175120743293 19.190140162581876 12-13 23.96600486038664 26.98281290784993 29.210001955526 19.84118027623743 14-15 21.19192747545819 28.57475733842374 28.188971087351277 22.044344098766793 16-17 19.163482148971255 31.338884220799162 29.373089666227514 20.124543964002065 18-19 18.801409245373222 29.97794823865863 32.351828961026094 18.868813554942047 20-21 20.141105462527666 29.062823332583825 32.10909548288497 18.68697572200354 22-23 19.59808172234938 29.483741416724023 31.57758264970069 19.340594211225902 24-25 19.231919172423776 29.645466028602595 32.153707498057024 18.968907300916598 26-27 19.20386016591201 29.695336896488296 32.08136124179411 19.01944169580559 28-29 18.73632375694796 29.77440875555234 32.28459163384368 19.204675853656013 30-31 19.459575644850457 29.528641689632686 32.1310023579115 18.880780307605363 32-33 19.090052325351003 29.64596395288134 32.010037783831 19.253945937936653 34-35 19.363619956901584 29.79889846200762 31.913934106715747 18.92354747437505 36-37 19.413975581454448 29.319483580410377 31.672523063060424 19.59401777507475 38-39 19.007474305057592 29.86233583338178 31.83550068799783 19.294689173562794 40-41 19.34682964917245 29.462261952904583 32.024356935305285 19.166551462617686 42-43 19.212261820831642 29.45585564478653 31.713968368678074 19.617914165703752 44-45 19.424286322084786 29.42701649645422 31.543030820826495 19.6056663606345 46-47 19.51799746858448 29.4677352185876 31.001183882488576 20.013083430339346 48-49 19.275474038250355 29.46388351772341 31.216371599478055 20.044270844548183 50-51 19.231898761213156 29.611001639773466 30.888242204186245 20.268857394827137 52-53 19.23314420950373 29.94766368340189 30.89789035640713 19.921301750687253 54-55 19.572516375346055 29.421881708229247 30.797535656290627 20.208066260134068 56-57 19.60462788963203 29.766504124416304 30.469566820022497 20.15930116592917 58-59 19.598047726826444 29.355753297712184 30.723283277696822 20.32291569776455 60-61 19.59980656499128 29.364140542677355 30.74617597676713 20.28987691556424 62-63 19.487963415500616 29.13492902495161 30.951666347298683 20.42544121224909 64-65 19.48968505473012 29.176221130447548 30.73482062153644 20.59927319328589 66-67 19.477536039050232 29.342103790053315 30.311599239044035 20.868760931852414 68-69 19.63355643948251 29.05680917297423 30.5605472021731 20.749087185370165 70-71 19.597349599321984 28.97865864162228 30.676932246484164 20.747059512571575 72-73 19.821710417236382 28.560613075000052 30.716194421775445 20.901482085988118 74-75 19.751522302703542 29.047473451213108 30.195207868603962 21.005796377479395 76-77 19.620113617963902 28.919828367284367 30.43496422308808 21.02509379166365 78-79 19.6334528430421 28.80122259896153 30.272156591880517 21.293167966115853 80-81 19.825651395805092 28.819064663317036 29.979967651978 21.375316288899874 82-83 20.04880888912622 28.80297211425087 30.117806244127127 21.03041275249578 84-85 19.99445379576964 28.541292308646266 30.06098671623802 21.403267179346074 86-87 19.93324925259573 28.68060271162532 30.044079951050023 21.342068084728925 88-89 20.474138209487847 28.36276060654479 30.00311377689078 21.159987407076585 90-91 20.573374411914568 28.298909993371318 29.971671973774715 21.156043620939393 92-93 20.70598125037413 28.344113212633708 29.91697504772675 21.03293048926541 94-95 20.738769484143937 28.118281329676538 29.90880697087887 21.234142215300654 96-97 21.092831127375085 27.877762364613822 29.575028779453987 21.454377728557102 98-99 21.19323398545095 27.76644692077262 29.321951564031494 21.718367529744935 100-101 21.62648431586552 27.538660749231443 29.765975092287505 21.068879842615534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2460.0 1 1512.5 2 703.5 3 827.0 4 676.5 5 678.5 6 1203.5 7 2274.5 8 3255.0 9 3260.0 10 2442.0 11 1779.5 12 1621.0 13 1592.5 14 1603.5 15 1643.5 16 1664.5 17 1755.0 18 1993.0 19 2256.0 20 2621.5 21 3011.0 22 3399.0 23 3961.0 24 4747.5 25 6144.5 26 8393.0 27 11149.5 28 13112.0 29 15410.5 30 19296.5 31 23276.0 32 27780.5 33 33306.5 34 39380.5 35 44427.0 36 48604.0 37 52562.0 38 52508.0 39 50597.0 40 49795.5 41 48733.5 42 47592.0 43 46862.5 44 46587.5 45 45760.5 46 43859.0 47 41975.0 48 41738.5 49 39938.0 50 35685.0 51 31025.0 52 26737.5 53 23051.0 54 19608.0 55 16639.0 56 13658.0 57 11020.0 58 8711.0 59 6657.5 60 4965.0 61 3597.5 62 2477.5 63 1620.0 64 1136.0 65 825.0 66 619.0 67 508.0 68 381.5 69 289.0 70 254.0 71 222.5 72 209.0 73 183.0 74 170.5 75 147.0 76 92.5 77 61.0 78 45.5 79 33.5 80 24.5 81 14.5 82 9.5 83 7.0 84 4.0 85 4.5 86 4.0 87 2.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30425290969294827 2 0.037719437997597326 3 0.04262813198358601 4 0.045728359764210455 5 0.060971146352280604 6 0.05778480113330549 7 0.03057169061449098 8 0.03470532765532357 9 0.03952790420296158 10-11 0.03225098066232922 12-13 0.033758035833466093 14-15 0.028332637217373332 16-17 0.021486300868494367 18-19 0.02583523150520365 20-21 0.038063907751000034 22-23 0.032035687066452524 24-25 0.05261775483226475 26-27 0.04559918360668443 28-29 0.037288850805843925 30-31 0.03720273336749325 32-33 0.03367191839511542 34-35 0.03216486322397854 36-37 0.03151898243634845 38-39 0.019591717224779433 40-41 0.01907501259467536 42-43 0.020280656731584863 44-45 0.009214565903522634 46-47 0.01674984175920703 48-49 0.014639964519615398 50-51 0.005942103246196839 52-53 0.005253163739391408 54-55 0.00727692354063236 56-57 0.0061143381228981955 58-59 0.008353391520015846 60-61 0.007879745609087113 62-63 0.0049517527051640324 64-65 0.008482567677541865 66-67 0.00292799290392308 68-69 0.008482567677541865 70-71 0.014898316834667434 72-73 0.014855258115492096 74-75 0.011625854177341641 76-77 0.010420210040432138 78-79 0.006200455561248875 80-81 0.007147747383106342 82-83 0.012573145999199108 84-85 0.004348930636709281 86-87 0.0016792900478382368 88-89 0.020280656731584863 90-91 0.02932298775840614 92-93 0.014510788362089383 94-95 0.022476651409527173 96-97 0.01898889515632468 98-99 0.014381612204563365 100-101 0.10704397586989377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1161205.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.720460341573045 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35315820665191 43.87506742355825 2 13.273551503206802 15.057639032589371 3 4.4343944428660675 7.545626824064302 4 1.8470806938205269 4.190690689661297 5 0.9337898861737399 2.6482496103039814 6 0.5349808151468275 1.8206614865422828 7 0.3083368081685516 1.224230398970008 8 0.2114948687434619 0.9596869052007782 9 0.1499537210984216 0.7654899681570893 >10 0.7906491250629594 8.71758416625626 >50 0.09948535620063975 3.9461747713457416 >100 0.06007507897484337 6.1274191331736425 >500 0.001982171025491235 0.7314846195582883 >1k 7.623734713427827E-4 1.1053564580958244 >5k 3.049493885371131E-4 1.284638512522915 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8521 0.7338066921861343 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6333 0.5453817370748489 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4900 0.42197544791832625 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3899 0.3357718921292967 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1428 0.12297570196476935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1310 0.11281384423938924 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1244 0.10713009330824445 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.611743835067882E-5 0.0 10-11 0.0 0.0 0.0 8.611743835067882E-5 0.0 12-13 0.0 0.0 0.0 8.611743835067882E-5 0.0 14-15 0.0 0.0 0.0 1.2917615752601824E-4 0.0 16-17 0.0 0.0 0.0 2.1529359587669708E-4 0.0 18-19 0.0 0.0 0.0 2.583523150520365E-4 0.0 20-21 0.0 0.0 0.0 9.903505410328065E-4 0.0 22-23 0.0 0.0 0.0 0.003401638814851814 0.0 24-25 0.0 0.0 0.0 0.00805198048578847 0.0 26-27 0.0 0.0 0.0 0.014338553485388025 0.0 28-29 0.0 0.0 0.0 0.026696405888710438 0.0 30-31 0.0 0.0 0.0 0.06437278516713242 0.0 32-33 0.0 0.0 0.0 0.12917615752601821 0.0 34-35 0.0 0.0 0.0 0.2289001511361043 0.0 36-37 0.0 0.0 0.0 0.3797779031264936 0.0 38-39 0.0 0.0 0.0 0.58103435655203 0.0 40-41 0.0 0.0 0.0 0.7868550342101523 0.0 42-43 0.0 0.0 0.0 0.9873364306905327 0.0 44-45 0.0 0.0 0.0 1.2409522866332818 0.0 46-47 0.0 0.0 0.0 1.546453899182315 0.0 48-49 0.0 0.0 0.0 1.852343040203926 0.0 50-51 0.0 0.0 0.0 2.1545721900956334 0.0 52-53 0.0 0.0 0.0 2.449179946693306 0.0 54-55 0.0 0.0 0.0 2.7880951253224024 0.0 56-57 0.0 0.0 0.0 3.179455823907062 0.0 58-59 0.0 0.0 0.0 3.5879108340043318 0.0 60-61 0.0 0.0 0.0 4.004676176902442 0.0 62-63 0.0 0.0 0.0 4.4158008275885825 0.0 64-65 0.0 0.0 0.0 4.8744623042442985 0.0 66-67 0.0 0.0 0.0 5.38397612824609 0.0 68-69 0.0 0.0 0.0 5.936505612704044 0.0 70-71 0.0 0.0 0.0 6.529251940871767 0.0 72-73 0.0 0.0 0.0 7.104171959300899 0.0 74-75 0.0 0.0 0.0 7.69222488707851 0.0 76-77 0.0 0.0 0.0 8.336943089290866 0.0 78-79 0.0 0.0 0.0 9.048402306225 0.0 80-81 0.0 0.0 0.0 9.791079094561253 0.0 82-83 0.0 0.0 0.0 10.524024612363881 0.0 84-85 0.0 0.0 0.0 11.256324249378878 0.0 86-87 0.0 0.0 0.0 12.037194121623658 0.0 88-89 0.0 0.0 0.0 12.85888365964666 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4470 0.0 33.5717 1 GTATCAA 6800 0.0 31.176067 1 TCAACGC 8150 0.0 25.896416 4 CAACGCA 8195 0.0 25.754217 5 ATCAACG 8285 0.0 25.474447 3 TATCAAC 8400 0.0 25.069101 2 AACGCAG 8440 0.0 25.058613 6 GTACATG 5020 0.0 23.914818 1 CATTCCG 285 3.6379788E-12 23.335482 9 TACATGG 5065 0.0 22.993282 2 GCACCGT 105 0.002189021 22.631855 6 CGCAGAG 9430 0.0 22.367815 8 ACGCAGA 9480 0.0 22.249842 7 CGCCGAC 55 0.0028831817 21.584528 92-93 ACATGGG 5110 0.0 21.581491 3 GAGTACT 5475 0.0 21.427965 12-13 AGTACTT 5595 0.0 21.265507 12-13 GCAGAGT 9835 0.0 20.914482 9 CATGGGT 1190 0.0 19.972729 4 GTACTTT 5890 0.0 19.880434 14-15 >>END_MODULE