FastQCFastQC Report
Thu 26 May 2016
SRR938413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161205
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78940.6798110583402586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63310.5452095021981476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.4265396721509122No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41770.35971253999078545No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT18970.16336478055123774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.12340628915652274No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.10962749902041413No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47400.034.8256341
CATTCCG2650.032.2599229
GAGCGGC753.080835E-431.6625187
GTATCAA69300.029.7923241
TAATGCG804.4699674E-429.714324
AGCACCG1109.41871E-525.9324955
TCAACGC84650.024.2067284
ATCAACG84750.024.1781643
TATCAAC85100.024.1491622
GTACATG50200.023.8805711
CAACGCA85900.023.8544755
AACGCAG88150.023.3293326
CATGGGG19200.023.2762174
TACATGG51800.022.7755992
ACATGGG50650.022.7154673
TCGTATC1053.4548066E-822.61608594-95
GAGTACT61200.022.03959812-13
TAGGACG3500.021.7339024
ATACGTC1100.002879571921.5880816
AGTACTT61850.021.42403212-13
Produced by FastQC (version 0.11.5)