##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938413_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1161205 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.805353921142263 31.0 31.0 34.0 30.0 34.0 2 32.03646126222329 33.0 31.0 34.0 30.0 34.0 3 32.09741949095982 33.0 31.0 34.0 30.0 34.0 4 35.77013102768245 37.0 35.0 37.0 35.0 37.0 5 35.64382430320228 37.0 35.0 37.0 33.0 37.0 6 35.68547586343497 37.0 35.0 37.0 33.0 37.0 7 35.62588431844507 37.0 35.0 37.0 33.0 37.0 8 35.626596509660224 37.0 35.0 37.0 33.0 37.0 9 37.18601108331431 39.0 37.0 39.0 33.0 39.0 10-11 37.13189402388036 39.0 37.0 39.0 33.0 39.0 12-13 37.04230217747943 39.0 37.0 39.0 33.0 39.0 14-15 38.08360711502276 40.0 37.0 41.0 33.0 41.0 16-17 38.02691557476931 40.0 37.0 41.0 33.0 41.0 18-19 38.08164320684117 40.0 37.0 41.0 33.0 41.0 20-21 38.111929418147525 40.0 37.0 41.0 33.0 41.0 22-23 38.10443203396471 40.0 37.0 41.0 33.0 41.0 24-25 38.03519662764111 40.0 37.0 41.0 33.0 41.0 26-27 37.86944682463476 40.0 37.0 41.0 33.0 41.0 28-29 37.741298909322644 40.0 37.0 41.0 32.5 41.0 30-31 37.49696823558287 39.0 36.5 41.0 32.0 41.0 32-33 37.25631219293751 39.0 36.0 41.0 31.5 41.0 34-35 37.0569076950237 39.0 35.5 41.0 31.0 41.0 36-37 37.01636231328663 39.0 36.0 41.0 31.0 41.0 38-39 36.865921607295874 39.0 35.0 41.0 31.0 41.0 40-41 36.70161211844592 39.0 35.0 41.0 30.5 41.0 42-43 36.47195757854987 39.0 35.0 41.0 30.0 41.0 44-45 36.15446066801297 39.0 35.0 40.5 30.0 41.0 46-47 35.76046434522759 39.0 35.0 40.0 28.5 41.0 48-49 35.37243337739676 38.5 35.0 40.0 27.0 41.0 50-51 35.060723989304215 38.0 34.5 40.0 26.0 41.0 52-53 34.63843378214871 38.0 34.0 40.0 24.0 41.0 54-55 34.37371179076907 38.0 33.5 40.0 24.0 41.0 56-57 34.06030459737945 38.0 33.0 40.0 23.0 41.0 58-59 33.67526233524657 38.0 33.0 40.0 22.0 41.0 60-61 33.33781933422608 37.0 32.5 40.0 20.5 41.0 62-63 32.916152617324244 37.0 32.0 39.5 18.5 41.0 64-65 32.48244194608188 36.0 31.0 39.0 15.5 40.0 66-67 32.020632015880054 36.0 31.0 39.0 12.5 40.0 68-69 31.493776292730395 35.5 30.5 38.5 8.0 40.0 70-71 30.99086509272695 35.0 29.5 38.0 7.5 40.0 72-73 30.446026756688095 34.5 29.0 37.5 4.5 39.0 74-75 29.852721095758284 34.0 28.5 37.0 2.0 39.0 76-77 28.477711084606078 32.5 27.0 35.0 2.0 37.5 78-79 29.124934443100052 34.0 28.5 36.0 2.0 38.0 80-81 28.81682519451776 34.0 28.0 35.5 2.0 37.0 82-83 28.35070551711369 33.5 27.5 35.0 2.0 37.0 84-85 27.786700453408315 33.0 26.0 35.0 2.0 36.0 86-87 27.17453421230532 33.0 26.0 35.0 2.0 36.0 88-89 26.47558958151231 32.0 25.0 35.0 2.0 35.5 90-91 25.97569335302552 32.0 23.5 34.0 2.0 35.0 92-93 25.273940863155083 31.5 20.5 34.0 2.0 35.0 94-95 24.54992486253504 31.0 17.0 34.0 2.0 35.0 96-97 23.630029581340075 31.0 4.5 34.0 2.0 35.0 98-99 22.526999108684514 31.0 2.0 34.0 2.0 35.0 100-101 19.33430358980542 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36987332028512654 1101 2 0.31106756700470584 1101 3 0.3576837976559446 1101 4 0.29506428257722916 1101 5 0.23828147739812522 1101 6 0.22262091929871275 1101 7 0.37178614131664034 1101 8 0.3269575114120755 1101 9 0.4086370607194638 1101 10-11 0.47467651672197064 1101 12-13 0.4365624219492332 1101 14-15 0.6816751874920115 1101 16-17 0.5066060555139913 1101 18-19 0.5957558286161841 1101 20-21 0.6506774048834885 1101 22-23 0.6219320587173698 1101 24-25 0.7070108073989303 1101 26-27 0.6695486816856047 1101 28-29 0.5450977312199967 1101 30-31 0.6435661235325796 1101 32-33 0.6431149489922277 1101 34-35 0.560896016854521 1101 36-37 0.6437237231573647 1101 38-39 0.842485666013701 1101 40-41 0.8190761925815195 1101 42-43 0.6462452662319578 1101 44-45 0.6303620692756979 1101 46-47 0.5658612571333563 1101 48-49 0.5889140404128952 1101 50-51 0.7849784025885924 1101 52-53 0.9955406832572677 1101 54-55 0.8701782279011852 1101 56-57 0.7637566444960342 1101 58-59 0.937146153799624 1101 60-61 0.8837389528330775 1101 62-63 1.0792333545260746 1101 64-65 0.9124176328608087 1101 66-67 0.9623839198021216 1101 68-69 0.903055354369549 1101 70-71 1.0195168304599456 1101 72-73 0.9257497242700872 1101 74-75 1.0079582051418683 1101 76-77 1.051166307326831 1101 78-79 1.22408125244392 1101 80-81 1.6015127837851253 1101 82-83 1.68124464495936 1101 84-85 1.9637253054621198 1101 86-87 2.1286853940517645 1101 88-89 2.097961175913891 1101 90-91 2.3609899215821315 1101 92-93 2.3872532831902227 1101 94-95 2.5435536441369457 1101 96-97 3.215266662918811 1101 98-99 3.4167041029223917 1101 100-101 4.533730436999537 1102 1 0.3009031337467114 1102 2 0.4033328764623647 1102 3 0.42028506303544333 1102 4 0.22674779762403574 1102 5 0.3103380692782167 1102 6 0.17795442383960136 1102 7 0.2489882985318701 1102 8 0.32337616058443786 1102 9 0.45327554039634776 1102 10-11 0.3542810272629069 1102 12-13 0.5144439618833943 1102 14-15 0.6568742162782257 1102 16-17 0.623041981085386 1102 18-19 0.6166906218830519 1102 20-21 0.7404936680450689 1102 22-23 0.6243857743104897 1102 24-25 0.6216513882594867 1102 26-27 0.5155249149636845 1102 28-29 0.4822396545034451 1102 30-31 0.6022638565888627 1102 32-33 0.5633283358668351 1102 34-35 0.7941997779732901 1102 36-37 0.917907179837961 1102 38-39 1.0495433084856387 1102 40-41 1.057376101530231 1102 42-43 0.9284902642475004 1102 44-45 0.816172075579253 1102 46-47 0.7518043384024651 1102 48-49 0.8093562228423252 1102 50-51 0.9539672663151606 1102 52-53 1.1710945786183302 1102 54-55 1.0681821034177332 1102 56-57 1.3347953296219899 1102 58-59 1.300034580946921 1102 60-61 1.1036558393458122 1102 62-63 1.3638083317632521 1102 64-65 1.4343399824510783 1102 66-67 1.1594119122378643 1102 68-69 1.1810279455959432 1102 70-71 1.3569996124271277 1102 72-73 1.472216233737086 1102 74-75 1.4713999439880858 1102 76-77 1.316001831338692 1102 78-79 1.6338120674697159 1102 80-81 1.7419792465591541 1102 82-83 1.6649511329475963 1102 84-85 1.89964620757031 1102 86-87 1.876120782654013 1102 88-89 2.0462627191165588 1102 90-91 2.2178526209027503 1102 92-93 2.5706399243313953 1102 94-95 2.7235760567627274 1102 96-97 3.5187639663997743 1102 98-99 3.850302029754509 1102 100-101 4.63678415726195 1103 1 0.31377913055517936 1103 2 0.32175001655401303 1103 3 0.2582171950392578 1103 4 0.22005691833977892 1103 5 0.15478144794116844 1103 6 0.20346574461900246 1103 7 0.24943738009466898 1103 8 0.14774777339765421 1103 9 0.20732010847144267 1103 10-11 0.3505449302612931 1103 12-13 0.4452073569400099 1103 14-15 0.6554589553531187 1103 16-17 0.551419139837904 1103 18-19 0.4056501914623496 1103 20-21 0.47759652516301543 1103 22-23 0.4831644468602718 1103 24-25 0.4324960539989178 1103 26-27 0.43275831093590256 1103 28-29 0.312192958740944 1103 30-31 0.3406838742905265 1103 32-33 0.4781519514530146 1103 34-35 0.4799795884870264 1103 36-37 0.5583275725009287 1103 38-39 0.6406480055299824 1103 40-41 0.4924262635047896 1103 42-43 0.729026546113765 1103 44-45 0.6279271124867805 1103 46-47 0.5538151774001889 1103 48-49 0.7892081567270566 1103 50-51 0.9002588554098878 1103 52-53 0.9360897608015577 1103 54-55 0.984814253297607 1103 56-57 1.1123329674529643 1103 58-59 1.3553845375647668 1103 60-61 1.270419879684674 1103 62-63 1.262129289920182 1103 64-65 1.139032666781624 1103 66-67 1.2858026480776203 1103 68-69 1.3324665326022256 1103 70-71 1.3356896022682854 1103 72-73 1.3112510135781186 1103 74-75 1.5212754655549006 1103 76-77 1.3475073009777283 1103 78-79 1.6063984720704063 1103 80-81 1.8195862086313817 1103 82-83 1.8418321524935415 1103 84-85 1.927767433432173 1103 86-87 2.2239750011866946 1103 88-89 2.390716125814418 1103 90-91 2.3877445361266147 1103 92-93 2.509839512745966 1103 94-95 2.386941465274351 1103 96-97 3.0443755675281423 1103 98-99 3.512842523395424 1103 100-101 4.072551977733966 1104 1 0.18114179319153223 1104 2 0.20527938966480264 1104 3 0.23022074346874888 1104 4 0.10035984907969464 1104 5 0.0887740882355601 1104 6 0.09811353013615332 1104 7 0.16602875215407664 1104 8 0.11910652619040007 1104 9 0.242226962197293 1104 10-11 0.24120976795349236 1104 12-13 0.25184875199848733 1104 14-15 0.3592460002998834 1104 16-17 0.4186750210312269 1104 18-19 0.43451814388711085 1104 20-21 0.35261706005590554 1104 22-23 0.3339702360572758 1104 24-25 0.3681561275959737 1104 26-27 0.4678861201092488 1104 28-29 0.5519481006781177 1104 30-31 0.4859152614636102 1104 32-33 0.4007965992385323 1104 34-35 0.5062470020762007 1104 36-37 0.5467409645366743 1104 38-39 0.4572024147821807 1104 40-41 0.6542362911036861 1104 42-43 0.6833142317491934 1104 44-45 0.5587795569604239 1104 46-47 0.5701100256062617 1104 48-49 0.7986123162749621 1104 50-51 0.8701846833767704 1104 52-53 0.9163996734050528 1104 54-55 0.8581554446499595 1104 56-57 0.9423126789787872 1104 58-59 1.0043105626666247 1104 60-61 0.7755646917493308 1104 62-63 0.8465055220137572 1104 64-65 0.76383388901845 1104 66-67 0.988461506009024 1104 68-69 1.072528876537035 1104 70-71 0.9300002048441876 1104 72-73 1.065589625747915 1104 74-75 1.2660554957822647 1104 76-77 1.0424532531396409 1104 78-79 1.3758207844636239 1104 80-81 1.7897978823072904 1104 82-83 1.6586461227918754 1104 84-85 1.8254248128512849 1104 86-87 1.8655080541502898 1104 88-89 1.9795036635986207 1104 90-91 2.2053864733062696 1104 92-93 2.42854022900303 1104 94-95 2.619323348570447 1104 96-97 2.9548294708005827 1104 98-99 3.2965994231194387 1104 100-101 3.946415166063577 1105 1 0.3832824707354341 1105 2 0.4327952696000352 1105 3 0.3266866794508161 1105 4 0.27401472515392555 1105 5 0.20015159477435418 1105 6 0.18643399345661749 1105 7 0.2751682776242461 1105 8 0.2711872647630713 1105 9 0.27420712784681456 1105 10-11 0.38338889985037383 1105 12-13 0.4146745241992633 1105 14-15 0.5395700599586277 1105 16-17 0.5634688932444547 1105 18-19 0.5362392486324126 1105 20-21 0.4917805611145667 1105 22-23 0.434741658401272 1105 24-25 0.40101358182439384 1105 26-27 0.7298069695300953 1105 28-29 0.5271026186753858 1105 30-31 0.5225165661092745 1105 32-33 0.5813650853513721 1105 34-35 0.6781558637028056 1105 36-37 0.7673677549215299 1105 38-39 0.7409348223049079 1105 40-41 0.6558600923242608 1105 42-43 0.7749601403305846 1105 44-45 0.6150155452528665 1105 46-47 0.7475620744292755 1105 48-49 0.6892361486326664 1105 50-51 0.7784262919207947 1105 52-53 0.6014176550964194 1105 54-55 0.5530034176229321 1105 56-57 0.6315170475178391 1105 58-59 0.7382761094345867 1105 60-61 0.9840795285907902 1105 62-63 1.0414371760179932 1105 64-65 1.2376335527374636 1105 66-67 1.0653507512450346 1105 68-69 1.2440854994041501 1105 70-71 1.2629748125163722 1105 72-73 1.2861012087595007 1105 74-75 1.2602445292858135 1105 76-77 1.208075528640947 1105 78-79 1.4986913941285884 1105 80-81 1.6061631675233805 1105 82-83 1.6476998654746495 1105 84-85 1.7799926295158741 1105 86-87 1.9186996097611981 1105 88-89 2.1868119367335055 1105 90-91 2.4042048193020342 1105 92-93 2.6422320025738344 1105 94-95 2.726115134596103 1105 96-97 3.1082709018629835 1105 98-99 3.8159379148369617 1105 100-101 4.558605351640857 1106 1 0.20719826289983345 1106 2 0.16271519121055178 1106 3 0.15604348094070275 1106 4 0.10826934795777987 1106 5 0.08397478631584221 1106 6 0.10867822721917264 1106 7 0.11279233978807923 1106 8 0.2580229435426773 1106 9 0.27376055731073734 1106 10-11 0.3629234927104079 1106 12-13 0.14110252287857605 1106 14-15 0.20593089808098597 1106 16-17 0.2158453027564846 1106 18-19 0.2885523622865165 1106 20-21 0.5097864068522142 1106 22-23 0.4330493310460568 1106 24-25 0.2718428640706705 1106 26-27 0.15008195655901346 1106 28-29 0.24332416026425818 1106 30-31 0.4296333180579808 1106 32-33 0.3775082983160658 1106 34-35 0.3690602032729089 1106 36-37 0.33222775087424594 1106 38-39 0.28864407521598423 1106 40-41 0.33554082862549706 1106 42-43 0.5121412075657048 1106 44-45 0.14846573247775297 1106 46-47 0.1961353310163858 1106 48-49 0.4559125531236248 1106 50-51 0.2966626502179537 1106 52-53 0.2613549526371699 1106 54-55 0.5254929228349496 1106 56-57 0.6400197657401989 1106 58-59 0.9773511074185564 1106 60-61 1.0040511976082271 1106 62-63 1.2635196706051346 1106 64-65 0.9782723083652414 1106 66-67 0.944621129544295 1106 68-69 0.9895562699551377 1106 70-71 0.9670039133956685 1106 72-73 1.2418580126838457 1106 74-75 1.603790786233521 1106 76-77 1.5010592740820492 1106 78-79 1.8444313242292658 1106 80-81 1.723985896518208 1106 82-83 1.7777903511638513 1106 84-85 1.8145858699442776 1106 86-87 2.061949091298132 1106 88-89 2.3506447443764813 1106 90-91 2.5307261641564303 1106 92-93 2.3844162574248706 1106 94-95 2.852574096513603 1106 96-97 3.274829159157605 1106 98-99 3.6178363341142017 1106 100-101 4.162876148362255 1107 1 0.2241582950383112 1107 2 0.23897711395886034 1107 3 0.18404238054726818 1107 4 0.18151974423041395 1107 5 0.10024197814381353 1107 6 0.02014677212352467 1107 7 0.1953575082603578 1107 8 0.23990138500131053 1107 9 0.23968838838578677 1107 10-11 0.29087392260618117 1107 12-13 0.22134900518567235 1107 14-15 0.3116509061738313 1107 16-17 0.4355164364964921 1107 18-19 0.3624752212134581 1107 20-21 0.3353966084891198 1107 22-23 0.4400370476860118 1107 24-25 0.45525996412641945 1107 26-27 0.4303682480730302 1107 28-29 0.4953477343013759 1107 30-31 0.43627679786298756 1107 32-33 0.4051629610876901 1107 34-35 0.5402314236179606 1107 36-37 0.4194637692548895 1107 38-39 0.5331868661106682 1107 40-41 0.7183834971086895 1107 42-43 0.7335972056560252 1107 44-45 0.6705922282341064 1107 46-47 0.6588402174327825 1107 48-49 0.6272842750501297 1107 50-51 0.6347723606077977 1107 52-53 0.9312896859154804 1107 54-55 0.8218986681695597 1107 56-57 0.7777272357522875 1107 58-59 0.7253842997093969 1107 60-61 0.8075560089184037 1107 62-63 0.9014538420423506 1107 64-65 0.7537764006352745 1107 66-67 0.985300389602493 1107 68-69 0.9900453337037263 1107 70-71 1.0161006010076612 1107 72-73 1.0342584569167492 1107 74-75 1.1204542506970725 1107 76-77 1.1526231864173724 1107 78-79 1.337862441201377 1107 80-81 1.4703899679739187 1107 82-83 1.5147955925881327 1107 84-85 1.5706678725438827 1107 86-87 1.7093392229814555 1107 88-89 2.0953818507784128 1107 90-91 2.220168136597106 1107 92-93 2.427683115932613 1107 94-95 2.5280813909867454 1107 96-97 2.678713213204972 1107 98-99 2.9683727759134335 1107 100-101 3.299468305584604 1108 1 0.04935724413536846 1108 2 0.1435863111283311 1108 3 0.008588256868890198 1108 4 0.07058240792378712 1108 5 -0.07965984324894038 1108 6 0.08083900205411965 1108 7 0.07169613737896441 1108 8 0.13156137354532405 1108 9 0.045357060076575806 1108 10-11 0.08022019675796344 1108 12-13 0.2197867326556846 1108 14-15 0.2725944330661463 1108 16-17 0.2308866267175489 1108 18-19 0.22961981016536726 1108 20-21 0.3040634012351191 1108 22-23 0.1502499494108065 1108 24-25 0.15537064635616815 1108 26-27 0.1693047870866522 1108 28-29 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-0.7314274882722387 2316 46-47 -0.6761599060566112 2316 48-49 -0.48038960909655515 2316 50-51 -0.32583135686620324 2316 52-53 -0.4494436257597201 2316 54-55 -0.27228542475019424 2316 56-57 -0.22497464882248863 2316 58-59 -0.3950504716104106 2316 60-61 -0.11188141140718955 2316 62-63 0.015156541647122879 2316 64-65 -0.15207212031010897 2316 66-67 0.154602763195264 2316 68-69 0.19843969929438998 2316 70-71 0.21555686771434424 2316 72-73 0.14749791298080694 2316 74-75 0.30186808391546904 2316 76-77 0.22812618404320872 2316 78-79 0.35020758075389224 2316 80-81 0.41654732639016245 2316 82-83 0.762560592907942 2316 84-85 1.0404671167888004 2316 86-87 0.9601848086789815 2316 88-89 1.0866543171402192 2316 90-91 1.318488490878675 2316 92-93 1.089205024533996 2316 94-95 1.7183437141198574 2316 96-97 2.1140570171722075 2316 98-99 2.2439181399 2316 100-101 2.744986052276829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 17.0 5 46.0 6 134.0 7 268.0 8 587.0 9 992.0 10 1644.0 11 2682.0 12 3937.0 13 5331.0 14 6899.0 15 9271.0 16 11437.0 17 14233.0 18 13273.0 19 10290.0 20 9790.0 21 10013.0 22 10942.0 23 12364.0 24 14008.0 25 16298.0 26 19003.0 27 22133.0 28 26792.0 29 32777.0 30 38854.0 31 46486.0 32 56843.0 33 68981.0 34 88059.0 35 115865.0 36 149083.0 37 195976.0 38 137019.0 39 8872.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.728988421510415 22.462872619391064 15.821065186594959 21.987073772503564 2 14.67288149466039 22.99156087243089 40.55425122666747 21.78130640624125 3 17.507259706304833 27.2187315923817 32.20123251383909 23.072776187474382 4 11.575475475906494 19.8033938882454 39.609199064764624 29.011931571083487 5 11.471827325179934 39.711743118791524 36.1555576819795 12.660871874049038 6 27.80482343772202 40.216843709767005 18.646492221442383 13.331840631068589 7 25.25850302056915 35.187326957772314 22.57534199387705 16.978828027781486 8 24.021942723291755 36.80495691975146 21.27238515163128 17.9007152053255 9 25.05449080911639 17.46513320214777 22.432387046214924 35.04798894252092 10-11 22.548688646707514 28.735064006786054 29.545644395261817 19.17060295124461 12-13 23.82563802257138 27.05030550161255 29.05839192907368 20.065664546742394 14-15 20.966452951890492 28.719304515567877 27.906054486503244 22.408188046038383 16-17 19.102785468543455 31.63059063645093 29.11156944725522 20.1550544477504 18-19 18.77261120990695 30.126549575656327 32.095021981476144 19.005817232960588 20-21 19.951300588612693 29.164316378245015 31.922744046055605 18.96163898708669 22-23 19.49612686821018 29.667715864123906 31.410086935554016 19.426070332111898 24-25 19.182589255342144 29.896416696305778 31.981215837876803 18.939778210475282 26-27 19.219155205186972 29.8542370224527 31.910542321992864 19.016065450367464 28-29 18.658290310496422 29.824707954237194 32.14458256724696 19.372419168019427 30-31 19.307788030537242 29.603127785360893 32.09213704729139 18.99694713681047 32-33 19.05057371882094 29.701747164130776 31.972423260155907 19.275255856892375 34-35 19.286920121961725 29.963695715836074 31.877142512618217 18.872241649583987 36-37 19.31219642426539 29.465362223982915 31.60882221227049 19.61361913948121 38-39 18.925081811968774 29.938766449061255 31.77521313745832 19.360938601511656 40-41 19.337092944442098 29.508583574553036 32.00627329123005 19.14805018977481 42-43 19.197787320239147 29.529654835048046 31.665383429533055 19.60717441517975 44-45 19.335942209713703 29.55086447736926 31.452189354819822 19.66100395809722 46-47 19.488073785580035 29.45799720548311 30.934139394291694 20.119789614645164 48-49 19.30395580453064 29.403206152229792 31.095499933258985 20.19733810998058 50-51 19.17620058473741 29.658458239501208 30.811570739016798 20.35377043674459 52-53 19.239884430397733 29.976963585241194 30.783281160518598 19.999870823842475 54-55 19.467328967464386 29.60912569663655 30.50216391686391 20.421381419035146 56-57 19.53212395744076 29.892482378219178 30.178650625858484 20.396743038481578 58-59 19.390805241107298 29.70500471492975 30.430759426630093 20.47343061733286 60-61 19.50628872593556 29.81863667483347 30.255596557024816 20.41947804220616 62-63 19.187998673791448 29.636239940406732 30.471277681374087 20.70448370442773 64-65 19.13947149728084 29.672667616829067 30.23324908177281 20.954611804117274 66-67 19.14637554291826 29.683909295673487 29.844604919990196 21.32511024141805 68-69 19.209378910283405 29.325672299627715 30.129452179252343 21.33549661083654 70-71 19.180463397935764 29.16479002415594 30.247673752696553 21.407072825211742 72-73 19.252888163588686 28.73170542669038 30.268255820462365 21.74715058925857 74-75 19.066486968278642 29.125951059459787 29.69725414547819 22.110307826783384 76-77 18.762406293462394 28.90798782299422 30.331810489965168 21.997795393578222 78-79 18.695536102583095 28.706128547500224 30.16396760261969 22.43436774729699 80-81 18.641109881545464 28.689593999336893 30.014295494766213 22.65500062435143 82-83 18.65110907680132 28.635642198398024 30.14258636176133 22.57066236303933 84-85 18.60129043814957 28.278026380606914 30.069086324318178 23.05159685692534 86-87 18.297652095769447 28.399276772984827 30.187278593539613 23.11579253770611 88-89 18.641117908172074 28.0596570199306 30.01917405590488 23.280051015992445 90-91 18.44402131602698 27.769481705006577 30.363841638592064 23.422655340374373 92-93 18.392450236909685 27.835854571383788 30.317334968420656 23.45436022328587 94-95 18.13361981734491 27.6594141430669 30.339561059416724 23.86740498017146 96-97 18.25108400325524 27.42168695449985 30.126075929745394 24.201153112499515 98-99 18.15949810756929 27.33126364423164 29.975284295193354 24.533953953005714 100-101 17.846371924071256 27.142277690032845 30.491631889245035 24.51971849665086 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2381.0 1 1471.0 2 658.0 3 736.5 4 628.0 5 701.0 6 1270.5 7 2313.5 8 3079.0 9 3037.0 10 2331.5 11 1667.5 12 1546.5 13 1522.5 14 1453.0 15 1449.0 16 1541.0 17 1721.5 18 1903.5 19 2041.5 20 2332.5 21 2728.5 22 3047.5 23 3526.0 24 4289.0 25 5709.0 26 7929.5 27 10552.5 28 12484.0 29 14764.0 30 18716.5 31 22883.0 32 27442.5 33 32883.0 34 39178.0 35 44553.0 36 49110.0 37 52913.5 38 52883.0 39 51365.5 40 50539.5 41 49325.5 42 48299.0 43 47929.5 44 47721.0 45 47025.0 46 45181.5 47 43280.5 48 43091.0 49 41238.5 50 36882.5 51 32010.5 52 26973.5 53 22736.5 54 19203.0 55 16024.0 56 13110.5 57 10558.0 58 8172.5 59 6080.5 60 4438.0 61 3123.0 62 2036.0 63 1332.5 64 875.0 65 563.5 66 397.5 67 328.5 68 256.5 69 164.5 70 132.0 71 119.0 72 103.5 73 96.5 74 105.0 75 95.5 76 50.0 77 17.0 78 9.0 79 7.5 80 7.0 81 5.5 82 4.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.046503416709366564 3 8.611743835067882E-5 4 0.0 5 0.10316869114411321 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012056441369095033 26-27 0.012228676245796391 28-29 0.0 30-31 0.0 32-33 7.750569451561094E-4 34-35 0.01593172609487558 36-37 0.003875284725780547 38-39 0.013649613978582593 40-41 0.00792280432826245 42-43 0.006803277629703627 44-45 0.003530814972377832 46-47 2.1529359587669705E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 4.305871917533941E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.0141103422737583E-4 68-69 3.444697534027153E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0012056441369095033 84-85 0.004219754479183262 86-87 0.001765407486188916 88-89 4.305871917533941E-5 90-91 0.0011195266985588247 92-93 2.583523150520365E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.47291821857467E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1161205.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.094914029504636 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17804426862394 41.97022799915634 2 13.940344753781698 15.360841915025173 3 4.58872165825746 7.58445675801062 4 1.9948219284268067 4.396181706033822 5 0.978721831032083 2.6961297569755986 6 0.5661379941835812 1.8714794471028362 7 0.3434876654353989 1.3247096378150987 8 0.21785068056680443 0.9601971613677732 9 0.16507700718038051 0.8185413166965885 >10 0.8552071888297581 9.114980173558552 >50 0.09984489699404125 3.8137633580747643 >100 0.06782418396383427 6.6172695577817935 >500 0.00250620334347289 0.8726596449742864 >1k 0.0010964639627693894 1.3709524446263366 >5k 3.1327541793411127E-4 1.2276091228005563 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7894 0.6798110583402586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6331 0.5452095021981476 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4953 0.4265396721509122 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4177 0.35971253999078545 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1897 0.16336478055123774 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1433 0.12340628915652274 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1273 0.10962749902041413 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.305871917533941E-5 0.0 16-17 0.0 0.0 0.0 1.2917615752601824E-4 0.0 18-19 0.0 0.0 0.0 3.014110342273759E-4 0.0 20-21 0.0 0.0 0.0 0.001033409260208146 0.0 22-23 0.0 0.0 0.0 0.003530814972377832 0.0 24-25 0.0 0.0 0.0 0.008396450239191186 0.0 26-27 0.0 0.0 0.0 0.014639964519615401 0.0 28-29 0.0 0.0 0.0 0.027040875642113153 0.0 30-31 0.0 0.0 0.0 0.06480337235888581 0.0 32-33 0.0 0.0 0.0 0.1295636859985963 0.0 34-35 0.0 0.0 0.0 0.2305363824647672 0.0 36-37 0.0 0.0 0.0 0.3831364832221701 0.0 38-39 0.0 0.0 0.0 0.5852541110312133 0.0 40-41 0.0 0.0 0.0 0.7924526677029464 0.0 42-43 0.0 0.0 0.0 0.9950870001420937 0.0 44-45 0.0 0.0 0.0 1.250252969975155 0.0 46-47 0.0 0.0 0.0 1.5567879917843963 0.0 48-49 0.0 0.0 0.0 1.8631507787169364 0.0 50-51 0.0 0.0 0.0 2.1651646350127667 0.0 52-53 0.0 0.0 0.0 2.45921262826116 0.0 54-55 0.0 0.0 0.0 2.799376509746341 0.0 56-57 0.0 0.0 0.0 3.194397199460905 0.0 58-59 0.0 0.0 0.0 3.6033258554691034 0.0 60-61 0.0 0.0 0.0 4.020607902997317 0.0 62-63 0.0 0.0 0.0 4.434057724518926 0.0 64-65 0.0 0.0 0.0 4.891255204722681 0.0 66-67 0.0 0.0 0.0 5.401371850792927 0.0 68-69 0.0 0.0 0.0 5.955150038106966 0.0 70-71 0.0 0.0 0.0 6.551771651000469 0.0 72-73 0.0 0.0 0.0 7.13168648085394 0.0 74-75 0.0 0.0 0.0 7.727274684487235 0.0 76-77 0.0 0.0 0.0 8.381508863637343 0.0 78-79 0.0 0.0 0.0 9.102742409824277 0.0 80-81 0.0 0.0 0.0 9.858810459824062 0.0 82-83 0.0 0.0 0.0 10.606051472392902 0.0 84-85 0.0 0.0 0.0 11.35673718249577 0.0 86-87 0.0 0.0 0.0 12.156208421424296 0.0 88-89 0.0 0.0 0.0 12.999427319034968 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4740 0.0 34.825634 1 CATTCCG 265 0.0 32.259922 9 GAGCGGC 75 3.080835E-4 31.662518 7 GTATCAA 6930 0.0 29.792324 1 TAATGCG 80 4.4699674E-4 29.71432 4 AGCACCG 110 9.41871E-5 25.932495 5 TCAACGC 8465 0.0 24.206728 4 ATCAACG 8475 0.0 24.178164 3 TATCAAC 8510 0.0 24.149162 2 GTACATG 5020 0.0 23.880571 1 CAACGCA 8590 0.0 23.854475 5 AACGCAG 8815 0.0 23.329332 6 CATGGGG 1920 0.0 23.276217 4 TACATGG 5180 0.0 22.775599 2 ACATGGG 5065 0.0 22.715467 3 TCGTATC 105 3.4548066E-8 22.616085 94-95 GAGTACT 6120 0.0 22.039598 12-13 TAGGACG 350 0.0 21.733902 4 ATACGTC 110 0.0028795719 21.588081 6 AGTACTT 6185 0.0 21.424032 12-13 >>END_MODULE