FastQCFastQC Report
Thu 26 May 2016
SRR938412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158851
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82370.7107902569010166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61550.53112954124387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46630.4023813242599782No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37640.3248044830612391No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13570.11709874694848603No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.1164947003540576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA300.00395453531.66214890-91
GCGTCCG452.4170815E-531.66078492-93
GTATCAA72850.031.559321
GGTATCA49650.031.1269021
CATTCCG2201.8189894E-1228.0690239
GTACATG53400.026.5292741
ATCAACG87900.026.0688083
TCAACGC88050.025.9164124
CAACGCA88500.025.7824065
TATCAAC89100.025.7710692
AACGCAG89100.025.5999376
ATGGGCG1309.961923E-625.5964745
GTGTAGC7850.024.9129071
TACATGG55400.024.7999632
ACATGGG53300.024.4391653
ACGCAGA98850.022.8747147
GAGTACT56750.022.80807112-13
CGCAGAG99150.022.709688
CATGGGG22650.022.6682784
GCAGAGT102100.021.6803769
Produced by FastQC (version 0.11.5)