##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938412_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1158851 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2644550507356 31.0 31.0 34.0 30.0 34.0 2 31.441280199093757 33.0 31.0 34.0 28.0 34.0 3 31.48267551220994 33.0 31.0 34.0 28.0 34.0 4 35.14238068569644 37.0 35.0 37.0 33.0 37.0 5 35.16270253898042 37.0 35.0 37.0 33.0 37.0 6 35.14840130439548 37.0 35.0 37.0 32.0 37.0 7 35.10517745594559 37.0 35.0 37.0 32.0 37.0 8 35.081282235593704 37.0 35.0 37.0 32.0 37.0 9 36.598240843732285 39.0 37.0 39.0 32.0 39.0 10-11 36.4721262699001 39.0 37.0 39.0 32.0 39.0 12-13 36.35154994041512 39.0 36.0 39.0 32.0 39.0 14-15 37.50043879670467 40.0 37.0 41.0 32.0 41.0 16-17 37.45356391805331 40.0 37.0 41.0 32.0 41.0 18-19 37.51038226657266 40.0 37.0 41.0 32.0 41.0 20-21 37.50410967415138 40.0 37.0 41.0 32.0 41.0 22-23 37.36606129692255 40.0 37.0 41.0 32.0 41.0 24-25 37.26586851976656 40.0 37.0 41.0 31.5 41.0 26-27 37.085463100950854 39.0 36.0 41.0 31.0 41.0 28-29 36.89166467475111 39.0 36.0 41.0 31.0 41.0 30-31 36.64003396467708 39.0 36.0 40.5 30.0 41.0 32-33 36.350081244266946 39.0 35.0 40.0 30.0 41.0 34-35 36.09622634833986 38.0 35.0 40.0 30.0 41.0 36-37 35.886324902856366 38.0 35.0 40.0 29.5 41.0 38-39 35.693730255226946 38.0 35.0 40.0 28.5 41.0 40-41 35.47730855821844 38.0 35.0 40.0 27.0 41.0 42-43 35.12549844630587 38.0 34.5 40.0 25.5 41.0 44-45 34.67811090468058 38.0 34.0 40.0 24.5 41.0 46-47 34.2032448520129 38.0 33.0 40.0 23.5 41.0 48-49 33.77028582621925 38.0 33.0 40.0 21.0 41.0 50-51 33.08776624432304 37.5 32.5 39.5 19.0 40.5 52-53 33.180666884698724 37.5 33.0 39.5 19.0 40.0 54-55 33.31776690877429 38.0 33.0 40.0 17.0 41.0 56-57 33.03176422162987 38.0 32.5 40.0 13.5 41.0 58-59 32.72072811776492 37.0 32.0 40.0 8.5 41.0 60-61 32.326642510555715 37.0 31.0 40.0 8.0 41.0 62-63 31.744125862600107 36.0 31.0 39.0 4.0 40.5 64-65 31.22382946556546 36.0 29.5 39.0 2.0 40.0 66-67 30.678945351904602 35.5 29.0 39.0 2.0 40.0 68-69 29.959122441107617 35.0 28.0 38.0 2.0 40.0 70-71 29.28032982669903 34.0 27.5 38.0 2.0 39.5 72-73 28.50456098324979 34.0 26.0 37.0 2.0 39.0 74-75 27.660071053137976 33.0 25.5 36.0 2.0 39.0 76-77 26.78079753134786 32.5 23.0 36.0 2.0 38.5 78-79 25.866301189712914 31.5 20.5 35.0 2.0 37.5 80-81 24.951193898093887 31.0 16.5 35.0 2.0 36.5 82-83 23.99564482405417 30.5 7.5 34.0 2.0 36.0 84-85 23.045719855270434 29.5 2.0 34.0 2.0 35.5 86-87 22.094289084619163 29.0 2.0 34.0 2.0 35.0 88-89 21.026885250994304 28.5 2.0 34.0 2.0 35.0 90-91 19.8673561139439 26.0 2.0 33.0 2.0 35.0 92-93 18.687147441733234 25.5 2.0 32.0 2.0 34.0 94-95 17.35809090210907 21.5 2.0 32.0 2.0 34.0 96-97 15.808295026711804 7.0 2.0 31.0 2.0 34.0 98-99 13.912753667210021 2.0 2.0 31.0 2.0 34.0 100-101 9.850207231128074 2.0 2.0 23.5 2.0 30.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6868508693518898 1101 2 0.6266583687456553 1101 3 0.5486783791587229 1101 4 0.3522719562859251 1101 5 0.3881997639865986 1101 6 0.4988557312290567 1101 7 0.37361798845599736 1101 8 0.5906602189167245 1101 9 0.6842684740657674 1101 10-11 0.6144856941223154 1101 12-13 0.6230368478969339 1101 14-15 0.794893750603606 1101 16-17 0.6930449060317372 1101 18-19 0.7767033689356353 1101 20-21 0.7351544155879779 1101 22-23 0.7443392610587338 1101 24-25 0.9023385393674914 1101 26-27 0.6959004326335503 1101 28-29 0.6361165635766994 1101 30-31 0.7073194763019899 1101 32-33 0.5818424467466841 1101 34-35 0.5625507803976362 1101 36-37 0.5821280758191634 1101 38-39 0.6483038363899567 1101 40-41 0.5845330929716468 1101 42-43 0.6421735111932136 1101 44-45 0.777749467481307 1101 46-47 0.6974056531690138 1101 48-49 0.5524064305564025 1101 50-51 0.5949069537509999 1101 52-53 0.7352262892544061 1101 54-55 0.9172610582057388 1101 56-57 0.9021324289736157 1101 58-59 0.842055117336777 1101 60-61 0.8413900606878215 1101 62-63 0.87585233658832 1101 64-65 1.00478092204526 1101 66-67 1.1954086472321244 1101 68-69 1.3714341175537896 1101 70-71 1.2877768168956543 1101 72-73 1.2951142244894704 1101 74-75 1.174367244436251 1101 76-77 1.521664732657655 1101 78-79 1.5037290497267115 1101 80-81 1.7821935862166356 1101 82-83 2.105875796627714 1101 84-85 2.078528129532824 1101 86-87 2.2601216214701942 1101 88-89 2.760432580993651 1101 90-91 2.911556402778583 1101 92-93 3.2751802953264573 1101 94-95 3.190486599900325 1101 96-97 3.2233697591463812 1101 98-99 3.1987071609463396 1101 100-101 2.849936532058514 1102 1 0.5296248821482727 1102 2 0.3049331969086282 1102 3 0.4504437775684167 1102 4 0.23359537228495952 1102 5 0.20437215734019532 1102 6 0.3331291717664797 1102 7 0.4431576955795933 1102 8 0.37058408161463774 1102 9 0.5399180858675052 1102 10-11 0.5189375683728414 1102 12-13 0.523431010036056 1102 14-15 0.5815740932123532 1102 16-17 0.6367545645064965 1102 18-19 0.619861619252724 1102 20-21 0.7200166409423687 1102 22-23 0.6291954770111516 1102 24-25 0.5888020882471423 1102 26-27 0.5093854396308615 1102 28-29 0.5447071858138557 1102 30-31 0.42651560041981895 1102 32-33 0.47654178999188446 1102 34-35 0.6558968245266854 1102 36-37 0.7934837513806556 1102 38-39 0.931506920487891 1102 40-41 0.920841080286344 1102 42-43 1.1044226300418885 1102 44-45 1.0238727203159712 1102 46-47 1.1045918053216113 1102 48-49 1.1051407539842373 1102 50-51 1.2100193250154945 1102 52-53 1.0742790253988659 1102 54-55 0.9494109035546856 1102 56-57 1.4673030277012629 1102 58-59 1.6188814064673096 1102 60-61 1.4909772785077067 1102 62-63 1.3059602599848326 1102 64-65 1.4500543908086634 1102 66-67 1.5951567986581345 1102 68-69 1.651245076335666 1102 70-71 1.598079572388567 1102 72-73 1.7649939180828085 1102 74-75 1.8174442767750243 1102 76-77 1.982546712555294 1102 78-79 2.097388311171205 1102 80-81 2.6541605454319175 1102 82-83 3.1285262342669675 1102 84-85 2.8043573587177555 1102 86-87 2.759675924156493 1102 88-89 2.904941854138272 1102 90-91 2.876537523240689 1102 92-93 3.3956105320877867 1102 94-95 3.4763620468496015 1102 96-97 3.4324905745311813 1102 98-99 3.417797665271747 1102 100-101 2.897815076581354 1103 1 0.6297584646492282 1103 2 0.5140391726506053 1103 3 0.5340136541465768 1103 4 0.5364004300731509 1103 5 0.49536621558356586 1103 6 0.5148223408868162 1103 7 0.5548428008249573 1103 8 0.5155390051650812 1103 9 0.7236390181318271 1103 10-11 0.6518421097410467 1103 12-13 0.5558852674213242 1103 14-15 0.655286909182152 1103 16-17 0.764521989422029 1103 18-19 0.7607151494040139 1103 20-21 0.7901347858841845 1103 22-23 0.8414099324025486 1103 24-25 0.6348343210319101 1103 26-27 0.6528817890456367 1103 28-29 0.5435517714416349 1103 30-31 0.6417992207938994 1103 32-33 0.9472711469524597 1103 34-35 0.8527907911231907 1103 36-37 0.8574299304755613 1103 38-39 1.0602969635680353 1103 40-41 0.8344706889481515 1103 42-43 0.8003083938516014 1103 44-45 0.6901121676041768 1103 46-47 0.7000504371187901 1103 48-49 0.6064346433492673 1103 50-51 0.7038661428426778 1103 52-53 0.7980589820828357 1103 54-55 0.7069176023370645 1103 56-57 0.8736581125292133 1103 58-59 0.9387354878975955 1103 60-61 0.8002378045728946 1103 62-63 1.0750023188152973 1103 64-65 1.018986106474511 1103 66-67 1.2635900043498403 1103 68-69 1.4551692916367607 1103 70-71 1.3898878810628688 1103 72-73 1.5470430159859383 1103 74-75 1.2774992648888919 1103 76-77 1.4299061211229223 1103 78-79 1.7316426560971259 1103 80-81 1.4635158635268866 1103 82-83 1.711436061273698 1103 84-85 2.0422006085205275 1103 86-87 2.0596018378511864 1103 88-89 2.5822728425701484 1103 90-91 2.24334161650906 1103 92-93 2.4860150450620644 1103 94-95 2.759224521524402 1103 96-97 3.0531752438205704 1103 98-99 3.328504883706655 1103 100-101 2.5211700854009447 1104 1 0.37381155966828317 1104 2 0.426422818398251 1104 3 0.3758270362850098 1104 4 0.28949009484635013 1104 5 0.3884720081067172 1104 6 0.467778473082447 1104 7 0.37939429848286466 1104 8 0.4042972819945021 1104 9 0.4670650130144267 1104 10-11 0.40494353771214975 1104 12-13 0.5225491758208918 1104 14-15 0.6428276544659184 1104 16-17 0.5893051003903693 1104 18-19 0.5754646581890981 1104 20-21 0.5299474508731805 1104 22-23 0.552679400495073 1104 24-25 0.6320119647903581 1104 26-27 0.7250956553410788 1104 28-29 0.5798448876182292 1104 30-31 0.6146854552563354 1104 32-33 0.4025016326183959 1104 34-35 0.5872155058453643 1104 36-37 0.6196267441903132 1104 38-39 0.7365198088341174 1104 40-41 0.5643426404545764 1104 42-43 0.6924025513551371 1104 44-45 0.7447043578142782 1104 46-47 0.8861004201635581 1104 48-49 0.8755246910348475 1104 50-51 0.8909310059271789 1104 52-53 0.9400367244899002 1104 54-55 0.9123399498170741 1104 56-57 1.0852313956296342 1104 58-59 1.1413342622535012 1104 60-61 1.1707018748989668 1104 62-63 1.1505200212112534 1104 64-65 1.1371389112265184 1104 66-67 1.4458554826901455 1104 68-69 1.5719478303717516 1104 70-71 1.6860255770012387 1104 72-73 1.5219468417023982 1104 74-75 1.5954933881574718 1104 76-77 1.7191156991242842 1104 78-79 1.7402115373277631 1104 80-81 1.9535771781818845 1104 82-83 2.0388504779245444 1104 84-85 2.1817145694082996 1104 86-87 2.487087401567969 1104 88-89 2.4792872618170136 1104 90-91 2.2363258830290498 1104 92-93 2.2392884590990505 1104 94-95 2.605957759778647 1104 96-97 2.8532272452678473 1104 98-99 3.111529859003939 1104 100-101 2.3199423320419434 1105 1 0.4853098175080035 1105 2 0.46624516106611935 1105 3 0.42617761915591856 1105 4 0.19099351464245018 1105 5 0.32679738342395837 1105 6 0.3792079048370809 1105 7 0.2611317269682587 1105 8 0.3870262362681771 1105 9 0.6263328755343238 1105 10-11 0.5916826868496727 1105 12-13 0.6519637239346281 1105 14-15 0.570312921382083 1105 16-17 0.57054999220437 1105 18-19 0.6585667788928404 1105 20-21 0.7232896025486681 1105 22-23 0.697224933251988 1105 24-25 0.7953126057332724 1105 26-27 0.5304361293807318 1105 28-29 0.5074860886334065 1105 30-31 0.6618420776162139 1105 32-33 0.6676379942994757 1105 34-35 0.8836139658617057 1105 36-37 0.5491176462421876 1105 38-39 0.6746408567112638 1105 40-41 0.7264226937947882 1105 42-43 0.9027200116725993 1105 44-45 0.5571586529921504 1105 46-47 0.6665290776247375 1105 48-49 0.5920902693890397 1105 50-51 0.635403410968685 1105 52-53 0.6851274888896057 1105 54-55 0.8020300173526635 1105 56-57 0.8444745237751974 1105 58-59 0.7758354882181422 1105 60-61 1.0026801946821635 1105 62-63 1.0607288653631919 1105 64-65 1.1580179493819749 1105 66-67 1.2584011875122734 1105 68-69 1.4929322584700024 1105 70-71 1.5167510469543508 1105 72-73 1.8183936950598216 1105 74-75 1.7568421643436452 1105 76-77 2.06542920194185 1105 78-79 2.093923844292103 1105 80-81 2.343955506568342 1105 82-83 2.531123674466034 1105 84-85 2.801203105563502 1105 86-87 2.7338342936400615 1105 88-89 2.98387925218136 1105 90-91 2.62154225503404 1105 92-93 2.950299557305268 1105 94-95 2.7403837072127217 1105 96-97 2.9748020721272272 1105 98-99 2.926167231568142 1105 100-101 2.5057458765853244 1106 1 0.38984906275241826 1106 2 0.14830692801983858 1106 3 0.023573104583753945 1106 4 0.13887463853141213 1106 5 0.14376072885312396 1106 6 -0.04938759503352941 1106 7 0.2536621019389216 1106 8 -0.09199261757691346 1106 9 0.1294230285676079 1106 10-11 0.2606082944995265 1106 12-13 0.30293590353058875 1106 14-15 0.3506412539030066 1106 16-17 0.019557953786566884 1106 18-19 0.41031721368067764 1106 20-21 0.2856199751121977 1106 22-23 0.26650363677180877 1106 24-25 0.33064626402914854 1106 26-27 0.21329826703245658 1106 28-29 0.32098542366377103 1106 30-31 0.15214284849956528 1106 32-33 0.13418415402457384 1106 34-35 0.16088750007003227 1106 36-37 0.42769072971544375 1106 38-39 0.3602621670324382 1106 40-41 0.3177123437379237 1106 42-43 -0.11399600115740327 1106 44-45 0.1969459529746942 1106 46-47 0.4412059204331342 1106 48-49 0.2385776650549971 1106 50-51 0.10026382752251806 1106 52-53 0.240415501984387 1106 54-55 -0.1141996047881193 1106 56-57 0.6322130553055842 1106 58-59 0.4335732073421852 1106 60-61 0.8592453406444989 1106 62-63 0.7956639932118783 1106 64-65 1.0214381500162553 1106 66-67 1.242256868037991 1106 68-69 1.0926092517240242 1106 70-71 0.7088573157224438 1106 72-73 1.086140260386319 1106 74-75 1.3724167462552685 1106 76-77 1.1529689948083224 1106 78-79 1.49825998794962 1106 80-81 1.2619724226738462 1106 82-83 1.4034021132015688 1106 84-85 1.3168088297184504 1106 86-87 1.1827061019362368 1106 88-89 1.2446165560754956 1106 90-91 1.270959579148066 1106 92-93 1.4823046062602714 1106 94-95 1.5698551014825526 1106 96-97 1.7633943825507021 1106 98-99 1.648039166238636 1106 100-101 1.4620135915570707 1107 1 0.09877050876516336 1107 2 0.1565511404805946 1107 3 0.01935315682262484 1107 4 0.0370726527737304 1107 5 -0.031737199458817145 1107 6 0.09087036628928757 1107 7 -0.010254792315649297 1107 8 0.055395013552008265 1107 9 0.10174238332398744 1107 10-11 0.09850614794142132 1107 12-13 0.10129088962008126 1107 14-15 0.04144712779803683 1107 16-17 -0.01716877907201564 1107 18-19 0.10001917987005271 1107 20-21 0.12168861732698844 1107 22-23 0.15130799625010383 1107 24-25 0.07726222325900522 1107 26-27 0.1973330816014851 1107 28-29 0.14765489795697562 1107 30-31 0.16009991733746176 1107 32-33 0.2939295958854373 1107 34-35 0.32292370994436936 1107 36-37 0.262347050703724 1107 38-39 0.33609105495709457 1107 40-41 0.3265818911995737 1107 42-43 0.41831935485814853 1107 44-45 0.16809120936203925 1107 46-47 0.08034721211149787 1107 48-49 0.03948503890323707 1107 50-51 0.22666657729492812 1107 52-53 0.39708531659596247 1107 54-55 0.5073195764058909 1107 56-57 0.6272401531556255 1107 58-59 0.6458042494821115 1107 60-61 0.8777352629668371 1107 62-63 0.89150402656308 1107 64-65 0.9337849004012391 1107 66-67 1.1431582863147902 1107 68-69 1.224444333442971 1107 70-71 1.4413926024832087 1107 72-73 1.3671241207877252 1107 74-75 1.6216595378868384 1107 76-77 1.8758541288512163 1107 78-79 1.9995468167779897 1107 80-81 2.287670713424845 1107 82-83 2.3087829719949724 1107 84-85 2.2560471121316468 1107 86-87 2.198165063795056 1107 88-89 2.4297220973093197 1107 90-91 2.3831816879308327 1107 92-93 2.154267477526343 1107 94-95 2.3493287991266953 1107 96-97 2.471788335093306 1107 98-99 2.5443888263456653 1107 100-101 2.2532878286058295 1108 1 0.2381891434992447 1108 2 0.0980331440814588 1108 3 0.08174524970590369 1108 4 0.1217048314019209 1108 5 0.09481837897612877 1108 6 0.1171416243212704 1108 7 0.06817487204371986 1108 8 0.08786482595861855 1108 9 0.1518574842484881 1108 10-11 0.18976322955220581 1108 12-13 -0.012965917438037877 1108 14-15 0.04423093341475237 1108 16-17 0.2636622971216198 1108 18-19 0.0979230758779508 1108 20-21 0.09966764572734377 1108 22-23 0.06584027994748709 1108 24-25 0.19032644218307126 1108 26-27 0.15466588166906803 1108 28-29 0.1778312716442656 1108 30-31 0.04124369317577248 1108 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-0.07097456834117821 2316 84-85 -0.0030561944585087986 2316 86-87 -0.10676408070159127 2316 88-89 -0.236454362116703 2316 90-91 0.01513156155224138 2316 92-93 -0.20037247632459554 2316 94-95 -0.45883191800538015 2316 96-97 -0.07462132382365816 2316 98-99 -0.5850688969168498 2316 100-101 -0.35452360796072035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5509.0 3 1150.0 4 1001.0 5 1022.0 6 1184.0 7 1489.0 8 1861.0 9 2530.0 10 3308.0 11 4525.0 12 6215.0 13 8176.0 14 9875.0 15 12151.0 16 14807.0 17 17873.0 18 16183.0 19 13852.0 20 14782.0 21 15950.0 22 18216.0 23 20886.0 24 23435.0 25 26652.0 26 30460.0 27 34623.0 28 39381.0 29 45418.0 30 52360.0 31 60732.0 32 70032.0 33 82645.0 34 98597.0 35 116505.0 36 132342.0 37 117604.0 38 35011.0 39 509.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.944712038256284 21.77281862914963 15.331710444244232 21.95075888834985 2 14.475083469427918 22.366871873133377 41.46091351980306 21.697131137635647 3 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29.367613905105834 31.038342959927363 20.074383132688183 48-49 19.35071537135701 29.402034646120544 31.17274813594343 20.074501846579018 50-51 19.305271715490992 29.607227680389954 30.82836805004177 20.259132554077286 52-53 19.252864015520423 29.908438667629213 30.87881014713072 19.959887169719646 54-55 19.58577652661734 29.462138612716416 30.79327531949395 20.158809541172293 56-57 19.569265077038388 29.72611210188994 30.45828638940298 20.246336431668695 58-59 19.602458926206758 29.2929271137303 30.7508596188207 20.353754341242244 60-61 19.64449837196237 29.301397963507448 30.750148650666187 20.303955013863995 62-63 19.430231910862133 29.087006596619748 30.98335677196719 20.499404720550928 64-65 19.474506551624003 29.15529853718441 30.737120806449315 20.633074104742274 66-67 19.42616772184806 29.22543947193899 30.361825172559158 20.986567633653795 68-69 19.61848405035676 28.94508151733042 30.58966733749176 20.846767094821068 70-71 19.626802199210573 28.841358138964875 30.699018761297797 20.832820900526762 72-73 19.82922594163925 28.45082176601158 30.62636141134558 21.09359088100359 74-75 19.73702606696424 28.95198790376868 30.119667767893205 21.191318261373876 76-77 19.64000932014705 28.78078668944925 30.423592053711662 21.155611936692036 78-79 19.613304606765183 28.62795541542268 30.16268080499404 21.596059172818098 80-81 19.83883760087753 28.63597095728256 29.87614642873479 21.649045013105127 82-83 19.983058402768997 28.698142249866553 29.958362335282235 21.360437012082215 84-85 20.054653792432095 28.29682195920858 29.8889707300592 21.75955351830013 86-87 19.93133523498287 28.490009436335374 29.913312567930177 21.665342760751578 88-89 20.543084252498574 28.136424835585938 29.805464933630226 21.51502597828526 90-91 20.571040895049077 28.135187833768217 29.77969895153515 21.51407231964756 92-93 20.76559182100663 28.017401893202397 29.72962943305333 21.487376852737643 94-95 20.69704806905144 27.91338275155369 29.561126673280892 21.828442506113984 96-97 21.025464867941643 27.61584639863011 29.230115942416745 22.128572791011496 98-99 21.130424163653633 27.452856201543863 28.987098213495894 22.42962142130661 100-101 21.872748166132418 26.8498518217714 29.413464778510267 21.863935233585913 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1971.0 1 1317.0 2 736.5 3 782.5 4 632.0 5 630.5 6 1109.0 7 2058.5 8 2953.0 9 3101.0 10 2430.0 11 1764.0 12 1599.0 13 1541.0 14 1513.0 15 1527.0 16 1566.0 17 1733.0 18 1889.5 19 2067.5 20 2366.0 21 2716.0 22 3154.5 23 3716.0 24 4520.5 25 5948.0 26 8264.5 27 10859.0 28 12909.0 29 15410.5 30 19061.0 31 23075.0 32 27738.0 33 33013.5 34 39066.0 35 44451.5 36 48740.0 37 52376.0 38 52172.0 39 50615.0 40 50225.5 41 49060.0 42 47445.5 43 46882.5 44 46487.5 45 45646.5 46 44174.0 47 42271.5 48 41612.5 49 39703.5 50 35639.5 51 31249.5 52 27118.0 53 23183.5 54 19901.0 55 16971.0 56 13819.5 57 11130.0 58 8807.5 59 6768.0 60 5072.0 61 3697.0 62 2610.5 63 1810.5 64 1255.0 65 879.5 66 688.0 67 532.0 68 394.5 69 301.0 70 261.0 71 242.5 72 202.0 73 162.0 74 155.5 75 141.5 76 90.0 77 50.5 78 34.5 79 21.0 80 10.0 81 8.5 82 8.5 83 6.0 84 5.5 85 2.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.5 91 2.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3909907313364704 2 0.02959828312699389 3 0.03529357958874782 4 0.04469944798770506 5 0.06480557034510909 6 0.05445048586919284 7 0.03943561337911431 8 0.04193809212746073 9 0.03909044389658377 10-11 0.03857268967278796 12-13 0.029943452609524437 14-15 0.027742997158392235 16-17 0.02222028543790358 18-19 0.027009512008014835 20-21 0.03516414103279887 22-23 0.03382660928799302 24-25 0.042326407795307594 26-27 0.03697628081608421 28-29 0.03175559239280977 30-31 0.03520728721811518 32-33 0.029511990756361257 34-35 0.03089266868648342 36-37 0.03348143980546248 38-39 0.015618919084506983 40-41 0.013030147965527924 42-43 0.01454026445159904 44-45 0.011865200961987347 46-47 0.02200455451132199 48-49 0.019156906280445026 50-51 0.011994639517936301 52-53 0.010311938290599914 54-55 0.009017552731110386 56-57 0.009535306954906196 58-59 0.004530349458213351 60-61 0.008111482839467714 62-63 0.01307329415084424 64-65 0.007982044283518761 66-67 0.003926302863784904 68-69 0.006817097279978186 70-71 0.01402251022780323 72-73 0.010959131070344678 74-75 0.012339809000466842 76-77 0.006126758314917104 78-79 0.00944901458427356 80-81 0.012426101371099476 82-83 0.0141950949690685 84-85 0.008844967989845114 86-87 0.002804502045560646 88-89 0.016481842790833333 90-91 0.02588771118979058 92-93 0.01091598488502836 94-95 0.020408145654618237 96-97 0.018596005871332897 98-99 0.011692616220722077 100-101 0.1260731534942801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1158851.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.34236037612296 #Duplication Level Percentage of deduplicated Percentage of total 1 77.79105393490521 44.60722648773752 2 13.13288469846701 15.061412143151323 3 4.286543437431174 7.374015556712498 4 1.8178873412367906 4.169678041775682 5 0.9042702714406264 2.5926495891181465 6 0.49996116736403434 1.7201372059833346 7 0.3050170330726179 1.224327764191411 8 0.18606589974003893 0.8535566301280701 9 0.13537820198847966 0.6986615080945471 >10 0.7836061592438506 8.734670959398647 >50 0.09533319449747349 3.821297626328709 >100 0.05897433802536303 6.091443546527103 >500 0.001965809681806754 0.7391236932736263 >1k 7.560806468487517E-4 1.0638560057219757 >5k 3.0243225873950063E-4 1.247943241857582 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8237 0.7107902569010166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6155 0.53112954124387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4663 0.4023813242599782 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3764 0.3248044830612391 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1357 0.11709874694848603 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.1164947003540576 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.629237063263525E-5 0.0 12-13 0.0 0.0 0.0 1.725847412652705E-4 0.0 14-15 0.0 0.0 0.0 2.588771118979058E-4 0.0 16-17 0.0 0.0 0.0 4.746080384794939E-4 0.0 18-19 0.0 0.0 0.0 8.19777521010035E-4 0.0 20-21 0.0 0.0 0.0 0.0012512393741732112 0.0 22-23 0.0 0.0 0.0 0.0034948410106217277 0.0 24-25 0.0 0.0 0.0 0.007377997689090314 0.0 26-27 0.0 0.0 0.0 0.012900709409578971 0.0 28-29 0.0 0.0 0.0 0.02493849511283159 0.0 30-31 0.0 0.0 0.0 0.061871629743599485 0.0 32-33 0.0 0.0 0.0 0.12404528278441318 0.0 34-35 0.0 0.0 0.0 0.21991610655727095 0.0 36-37 0.0 0.0 0.0 0.36652684426211823 0.0 38-39 0.0 0.0 0.0 0.563187156933894 0.0 40-41 0.0 0.0 0.0 0.7671391749241274 0.0 42-43 0.0 0.0 0.0 0.9735073792920745 0.0 44-45 0.0 0.0 0.0 1.2291053811059403 0.0 46-47 0.0 0.0 0.0 1.5308266550229495 0.0 48-49 0.0 0.0 0.0 1.83733715551007 0.0 50-51 0.0 0.0 0.0 2.141647200546058 0.0 52-53 0.0 0.0 0.0 2.4378457627425787 0.0 54-55 0.0 0.0 0.0 2.7789163576680695 0.0 56-57 0.0 0.0 0.0 3.177716548546793 0.0 58-59 0.0 0.0 0.0 3.597658370230513 0.0 60-61 0.0 0.0 0.0 4.025970551865598 0.0 62-63 0.0 0.0 0.0 4.447077320552858 0.0 64-65 0.0 0.0 0.0 4.894891577950919 0.0 66-67 0.0 0.0 0.0 5.404534318907262 0.0 68-69 0.0 0.0 0.0 5.967592037285208 0.0 70-71 0.0 0.0 0.0 6.563527148874186 0.0 72-73 0.0 0.0 0.0 7.14371390282271 0.0 74-75 0.0 0.0 0.0 7.731537531572221 0.0 76-77 0.0 0.0 0.0 8.37678873297775 0.0 78-79 0.0 0.0 0.0 9.074117380060077 0.0 80-81 0.0 0.0 0.0 9.791681588055756 0.0 82-83 0.0 0.0 0.0 10.50143633650918 0.0 84-85 0.0 0.0 0.0 11.221718754179786 0.0 86-87 0.0 0.0 0.0 11.97906374503711 0.0 88-89 0.0 8.629237063263525E-5 0.0 12.758413290405755 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGTA 30 0.003954535 31.662148 90-91 GCGTCCG 45 2.4170815E-5 31.660784 92-93 GTATCAA 7285 0.0 31.55932 1 GGTATCA 4965 0.0 31.126902 1 CATTCCG 220 1.8189894E-12 28.069023 9 GTACATG 5340 0.0 26.529274 1 ATCAACG 8790 0.0 26.068808 3 TCAACGC 8805 0.0 25.916412 4 CAACGCA 8850 0.0 25.782406 5 TATCAAC 8910 0.0 25.771069 2 AACGCAG 8910 0.0 25.599937 6 ATGGGCG 130 9.961923E-6 25.596474 5 GTGTAGC 785 0.0 24.912907 1 TACATGG 5540 0.0 24.799963 2 ACATGGG 5330 0.0 24.439165 3 ACGCAGA 9885 0.0 22.874714 7 GAGTACT 5675 0.0 22.808071 12-13 CGCAGAG 9915 0.0 22.70968 8 CATGGGG 2265 0.0 22.668278 4 GCAGAGT 10210 0.0 21.680376 9 >>END_MODULE