FastQCFastQC Report
Thu 26 May 2016
SRR938412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158851
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76190.657461571850048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62310.5376877614119503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48290.4167058577849957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41620.3591488465730279No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19950.17215327941210734No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.11856571724924085No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.11200749708116056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG2750.037.995919
GGTATCA45450.031.9988481
GACCGCC650.005847167729.2276237
GTATCAA65850.028.7981031
GTACATG53100.024.6141551
TACATGG54150.023.4235862
GGCATTC4900.023.2628027
TATCAAC82450.023.2196122
TCAACGC81850.023.1526684
ATCAACG81900.023.1395323
ACATGGG53550.022.8836783
CAACGCA83100.022.747255
AACGCAG84650.022.3307326
CATGGGG21800.021.1330474
GAGTACT54650.020.9446812-13
AGTACTT55750.020.9147912-13
TCGAGAC1604.9918104E-520.7799113
CCTATAC3451.8189894E-1220.6508433
ACGCAGA95700.019.7523167
CGCAGAG95950.019.7008518
Produced by FastQC (version 0.11.5)