##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938412_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1158851 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820484255525518 31.0 31.0 34.0 30.0 34.0 2 32.07535222388383 33.0 31.0 34.0 30.0 34.0 3 32.122073502115455 33.0 31.0 34.0 30.0 34.0 4 35.775275682551076 37.0 35.0 37.0 35.0 37.0 5 35.68016940918203 37.0 35.0 37.0 33.0 37.0 6 35.71251869308479 37.0 35.0 37.0 33.0 37.0 7 35.64015649984338 37.0 35.0 37.0 33.0 37.0 8 35.626760472226366 37.0 35.0 37.0 33.0 37.0 9 37.15546606077917 39.0 37.0 39.0 33.0 39.0 10-11 37.104011646018336 39.0 37.0 39.0 33.0 39.0 12-13 37.01514431104603 39.0 37.0 39.0 33.0 39.0 14-15 38.02446000391767 40.0 37.0 41.0 33.0 41.0 16-17 38.09613142673217 40.0 37.0 41.0 33.0 41.0 18-19 38.268615205923794 40.0 37.5 41.0 33.0 41.0 20-21 38.26520795166937 40.0 38.0 41.0 33.0 41.0 22-23 38.13335148349529 40.0 37.0 41.0 33.0 41.0 24-25 38.02604131160952 40.0 37.0 41.0 33.0 41.0 26-27 37.822065563217365 40.0 37.0 41.0 32.5 41.0 28-29 37.68479079709125 40.0 37.0 41.0 32.0 41.0 30-31 37.43074907818175 39.0 36.0 41.0 32.0 41.0 32-33 37.26278443044015 39.0 36.0 41.0 32.0 41.0 34-35 37.105089006265686 39.0 36.0 41.0 31.0 41.0 36-37 36.96755234279472 39.0 35.5 41.0 31.0 41.0 38-39 36.75834511943296 39.0 35.0 41.0 30.5 41.0 40-41 36.557953956116876 39.0 35.0 40.5 30.0 41.0 42-43 36.301781678576454 39.0 35.0 40.0 30.0 41.0 44-45 35.97502483062965 39.0 35.0 40.0 29.5 41.0 46-47 35.53105230957215 38.0 35.0 40.0 27.5 41.0 48-49 35.12669057540616 38.0 34.5 40.0 26.5 41.0 50-51 34.78379877999846 38.0 34.0 40.0 24.5 41.0 52-53 34.28940562678032 38.0 33.0 40.0 24.0 41.0 54-55 33.942404588683104 38.0 33.0 40.0 23.0 41.0 56-57 33.610900365965946 38.0 33.0 40.0 22.0 41.0 58-59 33.16718370178737 37.0 32.0 40.0 19.5 41.0 60-61 32.77078718489262 37.0 31.0 39.0 18.0 40.0 62-63 32.36712183015763 36.0 31.0 39.0 14.0 40.0 64-65 31.814277676767766 36.0 30.5 39.0 9.5 40.0 66-67 31.23984403516932 35.0 29.5 38.5 8.0 40.0 68-69 30.584146710836855 35.0 29.0 38.0 4.5 40.0 70-71 29.909314916240312 34.0 28.0 37.5 2.0 39.5 72-73 29.380080786917386 34.0 28.0 37.0 2.0 39.0 74-75 28.71834472248805 33.5 26.0 36.0 2.0 39.0 76-77 27.46395093070636 31.5 26.0 34.5 2.0 37.0 78-79 28.01298398154724 33.0 26.0 35.0 2.0 37.5 80-81 27.647470209716346 33.0 26.0 35.0 2.0 37.0 82-83 27.021731007696417 32.5 25.5 35.0 2.0 36.5 84-85 26.31004503598823 32.0 24.5 35.0 2.0 36.0 86-87 25.47336629126609 31.0 21.0 34.0 2.0 35.5 88-89 24.575676251735555 31.0 18.0 34.0 2.0 35.0 90-91 23.791962901184018 30.5 10.0 34.0 2.0 35.0 92-93 22.81926408140477 29.5 2.0 34.0 2.0 35.0 94-95 21.722563556488282 29.0 2.0 34.0 2.0 35.0 96-97 20.382965972329487 28.0 2.0 33.5 2.0 35.0 98-99 18.63978587411151 25.5 2.0 33.0 2.0 34.0 100-101 14.072091666659475 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2776430452438525 1101 2 0.3264091819651185 1101 3 0.3704475671342635 1101 4 0.3123662727879406 1101 5 0.21962910444032246 1101 6 0.3206756402478348 1101 7 0.3075597589069474 1101 8 0.3384882216904046 1101 9 0.3877960876054374 1101 10-11 0.41637743063417787 1101 12-13 0.49790926001559654 1101 14-15 0.5312885278704798 1101 16-17 0.560491791508646 1101 18-19 0.6112781421722744 1101 20-21 0.5665926162079984 1101 22-23 0.4870621398694297 1101 24-25 0.467019659955092 1101 26-27 0.5282754031013823 1101 28-29 0.5747224068170809 1101 30-31 0.606932137292894 1101 32-33 0.5582169098848127 1101 34-35 0.4468722423575855 1101 36-37 0.5219070398173287 1101 38-39 0.4315609599006507 1101 40-41 0.4390515811739135 1101 42-43 0.28375511862747516 1101 44-45 0.3871130255222468 1101 46-47 0.029738142607591556 1101 48-49 0.09144517339836256 1101 50-51 0.3768507965405732 1101 52-53 0.4278868989772704 1101 54-55 0.37493608159378056 1101 56-57 0.38190617995002185 1101 58-59 0.3690413562901327 1101 60-61 0.5020293428377158 1101 62-63 0.5488387750073471 1101 64-65 0.5969907587632726 1101 66-67 0.7791189493582102 1101 68-69 0.7528755135962406 1101 70-71 0.8824299284777517 1101 72-73 1.0614400910068689 1101 74-75 1.123075571134251 1101 76-77 1.1500573032094152 1101 78-79 1.3833817308552518 1101 80-81 1.2879474454495643 1101 82-83 1.7059154196603608 1101 84-85 1.953844485062742 1101 86-87 1.721297581784036 1101 88-89 1.730249474602335 1101 90-91 2.1114656466029906 1101 92-93 2.4582970492480634 1101 94-95 2.266242647626367 1101 96-97 2.187554124817062 1101 98-99 2.2734056499000097 1101 100-101 2.289089786998412 1102 1 0.24333937559342544 1102 2 0.20844280001663407 1102 3 0.20986724100583842 1102 4 0.22310024839887177 1102 5 0.2299543496648937 1102 6 0.24053935429499518 1102 7 0.053458569682717894 1102 8 0.11840206871834624 1102 9 0.26286608348080165 1102 10-11 0.2503736192164894 1102 12-13 0.28581925374214734 1102 14-15 0.4898883007879249 1102 16-17 0.32683668201460847 1102 18-19 0.3834735497144308 1102 20-21 0.30789514498256665 1102 22-23 0.3524456171097228 1102 24-25 0.27941933253373463 1102 26-27 0.40187901384145874 1102 28-29 0.3095261315536888 1102 30-31 0.4060788022113684 1102 32-33 0.27729330122071616 1102 34-35 0.39371043192508637 1102 36-37 0.35725807539207466 1102 38-39 0.43966136061850136 1102 40-41 0.5618924075941649 1102 42-43 0.6813348775047743 1102 44-45 0.6228828071533314 1102 46-47 0.5508566379990185 1102 48-49 0.6523263338321001 1102 50-51 0.8633501286774887 1102 52-53 0.9017687632394527 1102 54-55 0.8424416061462878 1102 56-57 1.1559262604577967 1102 58-59 1.1431265386527159 1102 60-61 0.990969311643461 1102 62-63 1.083316808366817 1102 64-65 1.0118106724646232 1102 66-67 1.2185965320125227 1102 68-69 1.1823533603000378 1102 70-71 1.7030829683701647 1102 72-73 1.5437560599000157 1102 74-75 1.249614000804673 1102 76-77 1.1932699112989802 1102 78-79 1.300474291124548 1102 80-81 1.4991364966833558 1102 82-83 1.58532220063314 1102 84-85 1.7383405011023818 1102 86-87 1.9418262452657231 1102 88-89 1.3935777363645983 1102 90-91 1.5093599885689208 1102 92-93 1.6330745875602766 1102 94-95 1.6346986592591115 1102 96-97 1.9473442145847812 1102 98-99 2.1149281592077536 1102 100-101 1.8482441548776105 1103 1 0.23722376076882412 1103 2 0.19249764485038412 1103 3 0.23300435920582174 1103 4 0.19793449339564262 1103 5 0.2571994137084914 1103 6 0.14157035958849207 1103 7 0.20351042135983732 1103 8 0.2373813551309354 1103 9 0.24481016754079832 1103 10-11 0.3636369899162375 1103 12-13 0.3346568064128803 1103 14-15 0.5256624633636875 1103 16-17 0.3813097737462172 1103 18-19 0.3071712076960509 1103 20-21 0.40008192284858524 1103 22-23 0.398280839731207 1103 24-25 0.2626131861660994 1103 26-27 0.38124711357140484 1103 28-29 0.36717191689037065 1103 30-31 0.15689196056822396 1103 32-33 0.14692411902694147 1103 34-35 0.14502376020867302 1103 36-37 0.20410429650998907 1103 38-39 0.2800713339631571 1103 40-41 0.38693209356608094 1103 42-43 0.18280437238119163 1103 44-45 0.1493656034920008 1103 46-47 0.2801026438472931 1103 48-49 0.16455887397018643 1103 50-51 0.1831513537225007 1103 52-53 0.10129026006239172 1103 54-55 0.1535438059329408 1103 56-57 0.4211792590747905 1103 58-59 0.5220233865774659 1103 60-61 0.5750345530224052 1103 62-63 0.3920251817792675 1103 64-65 0.6816675059215775 1103 66-67 0.5307174184971046 1103 68-69 0.4540316859495377 1103 70-71 0.4090625544478783 1103 72-73 0.8270025078198806 1103 74-75 0.39324838838263787 1103 76-77 0.5084866093736977 1103 78-79 0.6366229914253516 1103 80-81 0.8568353131371573 1103 82-83 1.1560235131314478 1103 84-85 1.0797202362263647 1103 86-87 1.155661338585638 1103 88-89 1.1458267537163458 1103 90-91 1.3159956053854636 1103 92-93 1.7040921647109464 1103 94-95 1.8638787038332048 1103 96-97 2.033054993181178 1103 98-99 1.8812640584783331 1103 100-101 1.6457356170424848 1104 1 0.14606627832032615 1104 2 0.1541082456820817 1104 3 0.13118049565242984 1104 4 0.14828000691196053 1104 5 0.08289153593541698 1104 6 0.14299492341722697 1104 7 0.1020272849181012 1104 8 0.18704947069907973 1104 9 0.10162608890747293 1104 10-11 0.05416550634171102 1104 12-13 0.13282101741057772 1104 14-15 0.30429611519573996 1104 16-17 0.1932048817161487 1104 18-19 0.28341928299349917 1104 20-21 0.20811221186629325 1104 22-23 0.12076063542474458 1104 24-25 0.185368321816064 1104 26-27 0.2445733565358026 1104 28-29 0.1835934222876503 1104 30-31 0.2757708385700681 1104 32-33 0.49018843078250995 1104 34-35 0.31912760734226 1104 36-37 0.4347238195244927 1104 38-39 0.4682192225097026 1104 40-41 0.426836105871196 1104 42-43 0.5534010829043083 1104 44-45 0.547180731451256 1104 46-47 0.5628631500055263 1104 48-49 0.5594624076348396 1104 50-51 0.63380189912926 1104 52-53 0.4845255126628629 1104 54-55 0.706706970411652 1104 56-57 0.8077374122689491 1104 58-59 0.7980241102169501 1104 60-61 0.8696831903573354 1104 62-63 0.7241728658895354 1104 64-65 0.9403142676348786 1104 66-67 0.6749796550623124 1104 68-69 0.8876511489311589 1104 70-71 1.178323776944307 1104 72-73 1.3281475942915542 1104 74-75 1.198365866223206 1104 76-77 1.1971703318660687 1104 78-79 1.2153094559597122 1104 80-81 1.3263309072110978 1104 82-83 1.3663559816669206 1104 84-85 1.4595113056065188 1104 86-87 1.4706554407615826 1104 88-89 1.4564932159467467 1104 90-91 1.9632671924761276 1104 92-93 1.9464988176511753 1104 94-95 2.0029322774927287 1104 96-97 2.3833094838833837 1104 98-99 2.7559198835328083 1104 100-101 2.439649662949785 1105 1 0.06355025474739406 1105 2 0.21639585268513173 1105 3 0.13137406901672932 1105 4 0.17519745862616531 1105 5 0.04468230463675127 1105 6 0.09852682000603608 1105 7 0.18258606669639477 1105 8 0.2092351290636003 1105 9 0.3248269320270154 1105 10-11 0.23852800472860025 1105 12-13 0.21308943914240785 1105 14-15 0.41348224673959066 1105 16-17 0.2615739185811137 1105 18-19 0.15376814521228255 1105 20-21 0.2832401853682569 1105 22-23 0.3140328856075598 1105 24-25 0.37733932882771626 1105 26-27 0.3124638370279058 1105 28-29 0.3788229142651076 1105 30-31 0.5135219462398766 1105 32-33 0.4726055168581311 1105 34-35 0.37236565956405343 1105 36-37 0.4198240475108932 1105 38-39 0.37129273737183155 1105 40-41 0.4851662129817882 1105 42-43 0.532962961856434 1105 44-45 0.41915883615293836 1105 46-47 0.40778400000768045 1105 48-49 0.48942648902168884 1105 50-51 0.467425592509052 1105 52-53 0.4367935472479729 1105 54-55 0.5929019633139632 1105 56-57 0.7599140372097963 1105 58-59 0.874636146178581 1105 60-61 0.8232094518104276 1105 62-63 0.9294627957729702 1105 64-65 1.0917262777867371 1105 66-67 0.8401676363072106 1105 68-69 1.166875349903325 1105 70-71 1.2931880605077808 1105 72-73 1.4046252043057486 1105 74-75 1.280150612642096 1105 76-77 1.3203797782462452 1105 78-79 1.5389426129262027 1105 80-81 1.6048130769534303 1105 82-83 1.8541393515641111 1105 84-85 1.8680693991239607 1105 86-87 1.995927518879192 1105 88-89 2.0815900026289 1105 90-91 2.4079105873227746 1105 92-93 2.7219537150572926 1105 94-95 2.3521171185474863 1105 96-97 2.860349532061644 1105 98-99 3.084419259796416 1105 100-101 2.7020168501137203 1106 1 0.03420583658078158 1106 2 0.015084568661855258 1106 3 0.028005892477821703 1106 4 0.1124811930475218 1106 5 0.12380316061215524 1106 6 0.13983768278157527 1106 7 0.06141265103921967 1106 8 -0.0010051025022974613 1106 9 -0.16819690457700176 1106 10-11 0.08652151149563991 1106 12-13 0.14358807610509672 1106 14-15 0.271499192934229 1106 16-17 0.0840699016830655 1106 18-19 0.20186560990268987 1106 20-21 0.16314095143735585 1106 22-23 0.14475749189137588 1106 24-25 0.12937184915292477 1106 26-27 -0.10803467957828161 1106 28-29 0.09143717771343773 1106 30-31 0.049735711412317585 1106 32-33 -0.16077656085605696 1106 34-35 -0.3018435982576193 1106 36-37 -0.1404993804828223 1106 38-39 -0.18256529010555766 1106 40-41 -0.05767468174840218 1106 42-43 0.009352778493315839 1106 44-45 0.07757431279475213 1106 46-47 -0.2723046726407006 1106 48-49 -0.3593403082317792 1106 50-51 -0.09010631700693494 1106 52-53 -0.005427060191259159 1106 54-55 -0.031792632077589644 1106 56-57 -0.059806976432611236 1106 58-59 0.18901934922600816 1106 60-61 0.09233033787494804 1106 62-63 -0.048085452137343054 1106 64-65 0.2906537509622815 1106 66-67 0.30749009543113104 1106 68-69 0.16480296148884221 1106 70-71 0.04624511550467858 1106 72-73 0.28713544965246385 1106 74-75 0.39688136914285366 1106 76-77 0.313730081845506 1106 78-79 0.5861298816160634 1106 80-81 0.38479011895183035 1106 82-83 0.4871558464218815 1106 84-85 0.8252646113425541 1106 86-87 0.9190452937282956 1106 88-89 0.7426187196888314 1106 90-91 1.0460956414531069 1106 92-93 1.1113355640941407 1106 94-95 1.0737280300674108 1106 96-97 1.1615852923837195 1106 98-99 1.5058467781056457 1106 100-101 1.9577683861634991 1107 1 0.011936253385705697 1107 2 0.07778397395551195 1107 3 -0.06066405826108934 1107 4 -0.13299469965009791 1107 5 -0.10198956848591934 1107 6 -0.017346863361339615 1107 7 -0.06965671435210652 1107 8 -0.0709022412756184 1107 9 -0.12628244359876106 1107 10-11 0.009739306743099974 1107 12-13 -0.016695494174896908 1107 14-15 0.13810077371304885 1107 16-17 0.06875982911017076 1107 18-19 -0.08073263575611378 1107 20-21 0.01679979928951525 1107 22-23 0.05488153793644557 1107 24-25 0.06984803962911457 1107 26-27 0.03983577823500184 1107 28-29 0.0709010912045116 1107 30-31 0.012330468807860484 1107 32-33 -0.031249263068978905 1107 34-35 0.08366266785433396 1107 36-37 0.07583249798948799 1107 38-39 0.05267765466928154 1107 40-41 0.028049388118951413 1107 42-43 -0.06792714187219673 1107 44-45 -0.18091024951328905 1107 46-47 0.13208713589618526 1107 48-49 0.26274039826915185 1107 50-51 0.3591713572573383 1107 52-53 0.3291250382968727 1107 54-55 0.11730722583949671 1107 56-57 0.22981671653215585 1107 58-59 0.4582898768045638 1107 60-61 0.6314967280329356 1107 62-63 0.7718223215849704 1107 64-65 0.6964110869500963 1107 66-67 0.607093624532606 1107 68-69 0.719175201719576 1107 70-71 0.5598387073098152 1107 72-73 0.8993937172618125 1107 74-75 0.8940418377612431 1107 76-77 0.9994418151720339 1107 78-79 0.9802398214647887 1107 80-81 0.9689295814189691 1107 82-83 1.1215709667669636 1107 84-85 1.3286805569596787 1107 86-87 1.2827031206253565 1107 88-89 1.2981735662477867 1107 90-91 1.4416774582427117 1107 92-93 1.2464440831136834 1107 94-95 1.3105555822424684 1107 96-97 1.3991977037945915 1107 98-99 1.4190592133388087 1107 100-101 1.5063041663858971 1108 1 0.045691711439864946 1108 2 0.04522440394722338 1108 3 0.01969935442851778 1108 4 0.033393454458426675 1108 5 0.03678096356546234 1108 6 -0.006260479589514034 1108 7 -0.053636302417515935 1108 8 0.03463817868436081 1108 9 -0.07408575829418851 1108 10-11 -0.11502969942905139 1108 12-13 0.027934493311832398 1108 14-15 0.12779316913223226 1108 16-17 0.004277572076084368 1108 18-19 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9 1081.0 10 1864.0 11 2822.0 12 4047.0 13 5653.0 14 7243.0 15 9809.0 16 12349.0 17 15679.0 18 14729.0 19 11764.0 20 11472.0 21 12354.0 22 13649.0 23 15339.0 24 17330.0 25 20236.0 26 23284.0 27 27058.0 28 32494.0 29 38828.0 30 45219.0 31 53610.0 32 64798.0 33 78368.0 34 98429.0 35 124921.0 36 156280.0 37 169177.0 38 66492.0 39 1338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.76326978750732 22.470380670744234 15.755946739586408 22.010402802162044 2 14.751323902041605 22.884345850853027 40.62402908096688 21.740301166138487 3 17.530166176086073 27.15555590073099 32.229807452510336 23.0844704706726 4 11.595882473242893 19.873650710919698 39.52656553776111 29.0039012780763 5 11.444219987625631 39.72974862082743 36.22005320805939 12.605978183487554 6 27.77673747530959 40.17876327500257 18.675135975203023 13.369363274484813 7 25.146459726056243 35.170181498743155 22.62335710112862 17.06000167407199 8 23.999634120348517 36.915962449012 21.272450038874712 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29.777011115358626 31.916594684199033 19.295817308065686 34-35 19.289002998985953 30.06917085589765 31.80012513754342 18.84170100757298 36-37 19.294810532106528 29.546549882964605 31.58661158272838 19.572028002200483 38-39 18.960869492046903 29.989009229744205 31.6951030012527 19.35501827695619 40-41 19.297124435202377 29.588901119907764 31.955624847082298 19.158349597807565 42-43 19.156728554150792 29.57810107296665 31.7007343394634 19.564436033419156 44-45 19.313576931573426 29.564446760921193 31.43992999944773 19.682046308057657 46-47 19.528109763990162 29.502912370022006 30.89545670276567 20.07352116322216 48-49 19.312836594178197 29.45715195482422 31.05049743237051 20.17951401862707 50-51 19.24121392655311 29.58706511881165 30.817853201144928 20.35386775349031 52-53 19.2573074536761 29.99143116759618 30.760943382712703 19.99031799601502 54-55 19.523087955224614 29.54573107327862 30.490761970261925 20.440419001234844 56-57 19.59755827108058 29.875713098577815 30.06909430116555 20.457634329176056 58-59 19.379670035233172 29.634094460806438 30.408007586825224 20.578227917135163 60-61 19.56295503045689 29.772852592783718 30.206687486139288 20.457504890620108 62-63 19.221107804195707 29.584907809545836 30.483470264943463 20.710514121314993 64-65 19.184665832506365 29.604219700565217 30.28152910212711 20.929585364801312 66-67 19.128787715354512 29.6447324706928 29.90369721576225 21.32278259819044 68-69 19.206690132641146 29.354933075374447 30.05761791307004 21.380758878914364 70-71 19.170635038773337 29.215847945873954 30.217055608122013 21.3964614072307 72-73 19.251826274098814 28.703795189794008 30.216633149199403 21.82774538690778 74-75 19.043431813063112 29.125875543965527 29.704552181427985 22.126140461543372 76-77 18.73411681053043 28.842405106437326 30.395150023600966 22.02832805943128 78-79 18.669699555853168 28.665937208493585 30.112715094520347 22.551648141132898 80-81 18.655666096273112 28.750337619482778 29.946524525628448 22.647471758615662 82-83 18.660931599006073 28.651478143360297 30.07441522114226 22.613175036491374 84-85 18.565250278201955 28.25112262417257 30.06268227675561 23.12094482086987 86-87 18.286899003715035 28.35684174645432 30.072402172928147 23.283857076902496 88-89 18.66111346497522 28.112199066143965 29.78376857766874 23.442918891212074 90-91 18.42585553299466 27.839026819083585 30.308481160886025 23.426636487035733 92-93 18.364845326530006 27.811194685740837 30.221095237540496 23.602864750188658 94-95 18.138016940062588 27.606408246792835 30.21735763154954 24.038217181595037 96-97 18.256790562376008 27.40455848077104 30.065772044896192 24.27287891195676 98-99 18.162861316942386 27.25604931091227 29.891892918071434 24.689196454073905 100-101 17.801080553065063 27.089763912703184 30.48385858061131 24.625296953620442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2175.0 1 1363.5 2 640.0 3 741.0 4 675.5 5 715.5 6 1182.5 7 2188.0 8 2980.0 9 2987.0 10 2334.5 11 1696.5 12 1534.5 13 1507.0 14 1499.0 15 1471.0 16 1535.0 17 1620.0 18 1753.5 19 1934.5 20 2183.0 21 2606.5 22 3053.0 23 3576.5 24 4385.0 25 5720.0 26 7888.0 27 10535.0 28 12495.5 29 14852.5 30 18771.0 31 22728.5 32 27267.5 33 32938.0 34 39302.5 35 44508.5 36 48943.0 37 52881.5 38 52867.5 39 51117.0 40 50407.0 41 49328.0 42 48150.5 43 47619.5 44 47517.0 45 47116.5 46 45330.0 47 43237.5 48 42774.5 49 40851.0 50 36549.0 51 31947.5 52 26957.0 53 22737.5 54 19245.5 55 16117.0 56 13274.5 57 10711.5 58 8185.0 59 6032.5 60 4445.5 61 3123.0 62 2172.5 63 1431.5 64 889.5 65 573.0 66 450.5 67 381.5 68 267.5 69 164.0 70 123.5 71 109.5 72 116.5 73 125.0 74 126.5 75 96.5 76 47.0 77 22.5 78 10.0 79 7.0 80 6.5 81 4.5 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027354681490545377 2 0.013375317448058465 3 0.001725847412652705 4 0.0 5 3.45169482530541E-4 6 0.0 7 0.0 8 0.0 9 4.3146185316317624E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018984321539179756 26-27 0.009621599325538832 28-29 2.588771118979058E-4 30-31 0.0 32-33 3.45169482530541E-4 34-35 0.010872838699712041 36-37 0.001164947003540576 38-39 0.01307329415084424 40-41 0.012037785703252618 42-43 0.004702934199478622 44-45 2.588771118979058E-4 46-47 8.629237063263525E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.629237063263525E-5 66-67 5.177542237958116E-4 68-69 8.629237063263525E-4 70-71 4.3146185316317626E-5 72-73 2.1573092658158812E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.725847412652705E-4 82-83 0.0013375317448058464 84-85 0.006083612129600785 86-87 0.003969449049101221 88-89 0.0 90-91 0.0010355084475916231 92-93 5.609004091121292E-4 94-95 4.3146185316317626E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1158851.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.24024781592971 #Duplication Level Percentage of deduplicated Percentage of total 1 76.22123654269156 42.104799954548795 2 13.888250779078367 15.34380829532136 3 4.608342266016375 7.6369790638610295 4 1.9673819717160983 4.347146706647587 5 0.9975662511665743 2.7552903463624774 6 0.577352415617761 1.9135854309510456 7 0.3334894289714213 1.2895427090261944 8 0.23296104337438825 1.0295060613967006 9 0.16277275318839712 0.8092446501427399 >10 0.8388790627568442 8.903217138605168 >50 0.10186771180473125 3.920069234837392 >100 0.06599039332467464 6.528758894879454 >500 0.002658378599105521 0.945969878527723 >1k 9.382512702725367E-4 1.2756901804490377 >5k 3.1275042342417895E-4 1.196391454443335 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7619 0.657461571850048 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6231 0.5376877614119503 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4829 0.4167058577849957 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4162 0.3591488465730279 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1995 0.17215327941210734 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1374 0.11856571724924085 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1298 0.11200749708116056 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.629237063263525E-5 0.0 12-13 0.0 0.0 0.0 1.725847412652705E-4 0.0 14-15 0.0 0.0 0.0 2.588771118979058E-4 0.0 16-17 0.0 0.0 0.0 4.746080384794939E-4 0.0 18-19 0.0 0.0 0.0 9.060698916426702E-4 0.0 20-21 0.0 0.0 0.0 0.0012512393741732112 0.0 22-23 0.0 0.0 0.0 0.0035379871959380456 0.0 24-25 0.0 0.0 0.0 0.007550582430355585 0.0 26-27 0.0 0.0 0.0 0.013375317448058465 0.0 28-29 0.0 0.0 0.0 0.025715126448525305 0.0 30-31 0.0 0.0 0.0 0.06282084582055847 0.0 32-33 0.0 0.0 0.0 0.12464932937884163 0.0 34-35 0.0 0.0 0.0 0.2209084688195463 0.0 36-37 0.0 0.0 0.0 0.36790752219224043 0.0 38-39 0.0 0.0 0.0 0.5653013200143935 0.0 40-41 0.0 0.0 0.0 0.7695985074871575 0.0 42-43 0.0 0.0 0.0 0.9771748050439617 0.0 44-45 0.0 0.0 0.0 1.2345418004557964 0.0 46-47 0.0 0.0 0.0 1.5374711675616624 0.0 48-49 0.0 0.0 0.0 1.8449308841257417 0.0 50-51 0.0 0.0 0.0 2.1498018295708423 0.0 52-53 0.0 0.0 0.0 2.4481145548478622 0.0 54-55 0.0 0.0 0.0 2.7900480734796793 0.0 56-57 0.0 0.0 0.0 3.1898406266206787 0.0 58-59 0.0 0.0 0.0 3.6085312089302253 0.0 60-61 0.0 0.0 0.0 4.036325636341514 0.0 62-63 0.0 0.0 0.0 4.4564400427664985 0.0 64-65 0.0 0.0 0.0 4.902701037493172 0.0 66-67 0.0 0.0 0.0 5.410057030627751 0.0 68-69 0.0 0.0 0.0 5.9738050879707565 0.0 70-71 0.0 0.0 0.0 6.574313695203266 0.0 72-73 0.0 0.0 0.0 7.160497768910757 0.0 74-75 0.0 0.0 0.0 7.761437837996429 0.0 76-77 0.0 0.0 0.0 8.418640532734578 0.0 78-79 0.0 0.0 0.0 9.13137236797483 0.0 80-81 0.0 0.0 0.0 9.86274335527173 0.0 82-83 0.0 0.0 0.0 10.592863103194457 0.0 84-85 0.0 0.0 0.0 11.342700657806741 0.0 86-87 0.0 0.0 0.0 12.128176961490304 0.0 88-89 0.0 0.0 0.0 12.944761664786931 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 275 0.0 37.99591 9 GGTATCA 4545 0.0 31.998848 1 GACCGCC 65 0.0058471677 29.227623 7 GTATCAA 6585 0.0 28.798103 1 GTACATG 5310 0.0 24.614155 1 TACATGG 5415 0.0 23.423586 2 GGCATTC 490 0.0 23.262802 7 TATCAAC 8245 0.0 23.219612 2 TCAACGC 8185 0.0 23.152668 4 ATCAACG 8190 0.0 23.139532 3 ACATGGG 5355 0.0 22.883678 3 CAACGCA 8310 0.0 22.74725 5 AACGCAG 8465 0.0 22.330732 6 CATGGGG 2180 0.0 21.133047 4 GAGTACT 5465 0.0 20.94468 12-13 AGTACTT 5575 0.0 20.91479 12-13 TCGAGAC 160 4.9918104E-5 20.779911 3 CCTATAC 345 1.8189894E-12 20.650843 3 ACGCAGA 9570 0.0 19.752316 7 CGCAGAG 9595 0.0 19.700851 8 >>END_MODULE