FastQCFastQC Report
Thu 26 May 2016
SRR938411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853609
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67080.23507074725374077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.1866408467312796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.1310971475068939No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37850.055.291761
GTATCAA70200.041.8043671
ATCAACG91050.032.0218543
TCAACGC92850.031.4010774
CAACGCA93700.031.0147025
TATCAAC94450.030.7684212
AACGCAG98150.029.6825226
GTACATG89750.026.285781
TACATGG89350.025.2851222
ACGCAGA114500.025.0267457
CGCAGAG116100.024.5586178
ACATGGG94100.023.1495113
TGGTATC19750.021.9149382
GCAGAGT127000.021.6642939
GTGGTAT23350.020.9808921
GGACCGA7200.020.4625516
GAGTACT72250.020.22339612-13
AGAGTAC117000.020.06351310-11
CATGGGG56450.019.5474474
TAAGGTG12000.019.4214125
Produced by FastQC (version 0.11.5)