##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938411_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2853609 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.127975836913887 31.0 31.0 34.0 28.0 34.0 2 31.16322102993087 31.0 31.0 34.0 27.0 34.0 3 31.212536125306585 31.0 31.0 34.0 27.0 34.0 4 34.865697087442605 37.0 35.0 37.0 32.0 37.0 5 34.84158201070995 37.0 35.0 37.0 32.0 37.0 6 34.8542396663313 37.0 35.0 37.0 32.0 37.0 7 34.76359410136427 37.0 35.0 37.0 32.0 37.0 8 34.738003699876195 37.0 35.0 37.0 31.0 37.0 9 36.23530939242202 39.0 35.0 39.0 32.0 39.0 10-11 36.07201652363726 39.0 35.0 39.0 30.0 39.0 12-13 35.935822672272195 38.0 35.0 39.0 30.0 39.0 14-15 36.90880653235955 39.0 36.0 41.0 31.0 41.0 16-17 36.732973578370405 39.0 36.0 41.0 30.0 41.0 18-19 36.88541212198307 39.0 36.0 41.0 30.5 41.0 20-21 36.86851317051495 39.0 36.0 41.0 30.0 41.0 22-23 36.7766687727716 39.0 36.0 41.0 30.0 41.0 24-25 36.64613512222593 39.0 36.0 41.0 30.0 41.0 26-27 36.4447576735285 39.0 36.0 41.0 29.5 41.0 28-29 36.24125677344023 39.0 35.0 40.5 29.0 41.0 30-31 36.0019559442096 39.0 35.0 40.0 27.5 41.0 32-33 35.75116352660788 38.0 35.0 40.0 27.0 41.0 34-35 35.510452027590325 38.0 34.0 40.0 27.0 41.0 36-37 35.24294148217223 38.0 34.0 40.0 25.5 41.0 38-39 35.13431763076161 38.0 34.0 40.0 25.0 41.0 40-41 35.04001476726489 38.0 34.0 40.0 25.0 41.0 42-43 34.7720015250863 38.0 33.5 40.0 24.5 41.0 44-45 34.44798166111755 38.0 33.0 40.0 24.0 41.0 46-47 34.09423365289358 38.0 33.0 40.0 23.0 41.0 48-49 33.76055952304608 38.0 33.0 40.0 21.5 41.0 50-51 33.08277815916617 37.5 31.5 39.5 20.0 40.5 52-53 33.11998209986021 37.0 32.0 39.5 19.5 40.0 54-55 33.23531920455816 37.0 32.0 40.0 18.5 41.0 56-57 32.93194389981248 37.0 31.5 40.0 16.5 41.0 58-59 32.56444628538809 37.0 31.0 40.0 13.5 41.0 60-61 32.09919771769714 36.0 31.0 39.0 10.0 41.0 62-63 31.53282474929116 35.5 30.0 39.0 8.0 40.0 64-65 31.020870764004457 35.0 29.0 39.0 6.0 40.0 66-67 30.489205598945055 35.0 29.0 38.0 2.0 40.0 68-69 29.898806914331992 34.5 28.0 38.0 2.0 40.0 70-71 29.261020167794534 34.0 27.5 37.0 2.0 39.0 72-73 28.619945654783116 34.0 26.0 36.5 2.0 39.0 74-75 27.929009019806145 33.5 26.0 36.0 2.0 39.0 76-77 27.170508643615857 32.5 24.5 35.5 2.0 38.0 78-79 26.373567296710938 32.0 22.5 35.0 2.0 37.0 80-81 25.655968459589243 31.5 20.5 35.0 2.0 36.5 82-83 24.912075550644815 31.0 18.0 34.5 2.0 36.0 84-85 24.184269814119595 31.0 10.5 34.0 2.0 35.5 86-87 23.397481575086147 30.0 4.5 34.0 2.0 35.0 88-89 22.57397159176327 29.5 2.0 34.0 2.0 35.0 90-91 21.643556983454985 29.0 2.0 34.0 2.0 35.0 92-93 20.68727460559593 28.0 2.0 34.0 2.0 35.0 94-95 19.66526142859796 26.5 2.0 33.0 2.0 35.0 96-97 18.39641906091549 25.0 2.0 33.0 2.0 34.5 98-99 16.85645913648296 11.0 2.0 32.0 2.0 34.0 100-101 13.298499198733953 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38192487156807786 1101 2 0.30454777882443196 1101 3 0.3543725471592154 1101 4 0.1734602091132018 1101 5 0.30093714061270305 1101 6 0.39378281031481066 1101 7 0.3414803976236058 1101 8 0.5341699622971632 1101 9 0.5982041489224272 1101 10-11 0.629229535156206 1101 12-13 0.5910236338152117 1101 14-15 0.7653681667489423 1101 16-17 0.7178071949012406 1101 18-19 0.7543803306341701 1101 20-21 0.8604226428248651 1101 22-23 0.80255653145921 1101 24-25 0.7570245343184681 1101 26-27 0.7095504302270186 1101 28-29 0.6680779677707633 1101 30-31 0.6447453058225747 1101 32-33 0.6707375064410783 1101 34-35 0.7167380714460023 1101 36-37 0.8673733308802127 1101 38-39 0.878860057264589 1101 40-41 0.785952272404252 1101 42-43 0.8931657364225885 1101 44-45 0.9665984651895911 1101 46-47 0.9614236143287371 1101 48-49 0.8344434983061717 1101 50-51 0.9549024915296442 1101 52-53 0.9083268780608407 1101 54-55 1.0149819194308947 1101 56-57 0.9711258085704841 1101 58-59 0.887897473631746 1101 60-61 1.0216597761173603 1101 62-63 0.8594686337553306 1101 64-65 0.7145003646591839 1101 66-67 0.6955991754975805 1101 68-69 0.7402791607582522 1101 70-71 0.7754618145134806 1101 72-73 1.145683030058695 1101 74-75 0.9162119908880584 1101 76-77 0.9434741440922565 1101 78-79 1.1223305138023996 1101 80-81 1.0612228835823636 1101 82-83 1.1098661101253775 1101 84-85 1.1709265285685326 1101 86-87 1.208181078347664 1101 88-89 1.0690749301398839 1101 90-91 0.8468078030214841 1101 92-93 0.9526694042318518 1101 94-95 0.9843181101473988 1101 96-97 0.9418970430869571 1101 98-99 1.0957201279475655 1101 100-101 0.9772161326543802 1102 1 0.42141681141871246 1102 2 0.42909280910857817 1102 3 0.3719108479975084 1102 4 0.33730161017609817 1102 5 0.3548557106035659 1102 6 0.2684053481090416 1102 7 0.27879912038068966 1102 8 0.5031098541176533 1102 9 0.5498167220428982 1102 10-11 0.7042690595645595 1102 12-13 0.6798279740336355 1102 14-15 0.7107365529326657 1102 16-17 0.7357783881328288 1102 18-19 0.872064903999231 1102 20-21 0.8242917621399428 1102 22-23 0.9175671904789766 1102 24-25 0.8177395085672714 1102 26-27 0.729327542126633 1102 28-29 0.5946786715667471 1102 30-31 0.7679224896329941 1102 32-33 0.779784319461136 1102 34-35 0.5357226611590207 1102 36-37 0.9763941185586447 1102 38-39 0.9561803364887069 1102 40-41 0.9723294715772042 1102 42-43 1.0329016701676252 1102 44-45 1.0606018727406266 1102 46-47 1.0508019017652472 1102 48-49 1.2313952738535079 1102 50-51 1.2322444501159993 1102 52-53 1.0064476588918367 1102 54-55 0.9175213737224368 1102 56-57 1.1527772605192368 1102 58-59 1.1404824470037838 1102 60-61 0.9791742902987721 1102 62-63 1.1009580312080338 1102 64-65 1.1567386349998223 1102 66-67 0.9835853724649475 1102 68-69 0.9198514911204008 1102 70-71 1.3570202448164785 1102 72-73 1.2917632093353966 1102 74-75 1.1260212200806095 1102 76-77 1.0802405989934982 1102 78-79 1.1617777936614893 1102 80-81 1.4678895022411176 1102 82-83 1.4475393586767389 1102 84-85 1.5544407001250597 1102 86-87 1.7743885367052243 1102 88-89 1.6523114896301507 1102 90-91 1.6086719477062132 1102 92-93 1.9066248897800797 1102 94-95 1.977975633328274 1102 96-97 1.9994916925799266 1102 98-99 2.131228042809827 1102 100-101 1.8803975032600455 1103 1 0.43999884954004287 1103 2 0.43789747749124786 1103 3 0.3614851728658941 1103 4 0.2987934861675612 1103 5 0.3111688063481779 1103 6 0.40371604938086136 1103 7 0.4179302014062145 1103 8 0.463146266256949 1103 9 0.5747972789627696 1103 10-11 0.6221755950910861 1103 12-13 0.6970217506910927 1103 14-15 0.8124275717987217 1103 16-17 0.690259747467465 1103 18-19 0.8028955839025187 1103 20-21 0.5907750787973498 1103 22-23 0.7574420833504618 1103 24-25 0.8379314848088839 1103 26-27 0.8917308406266287 1103 28-29 0.9149527259470389 1103 30-31 0.884633880857443 1103 32-33 0.9785638754364072 1103 34-35 1.0793653517164117 1103 36-37 0.9994784126124614 1103 38-39 0.9900334331625942 1103 40-41 1.023610793059568 1103 42-43 0.9672466396705133 1103 44-45 1.0755164241437072 1103 46-47 0.956666559925857 1103 48-49 0.9480235408930611 1103 50-51 1.132197183422619 1103 52-53 1.254372595101266 1103 54-55 1.1664608531368543 1103 56-57 1.1676595791025903 1103 58-59 1.0042172285516315 1103 60-61 1.06505845600514 1103 62-63 1.1929250773462066 1103 64-65 1.29594622335971 1103 66-67 1.1134366855083861 1103 68-69 1.3616003393140375 1103 70-71 1.2674557600806047 1103 72-73 1.3816996486475652 1103 74-75 1.4918985110738845 1103 76-77 1.5387514865102006 1103 78-79 1.4779917899059392 1103 80-81 1.6867285149972844 1103 82-83 1.8118513509642185 1103 84-85 2.0416561299393727 1103 86-87 2.0695430073243415 1103 88-89 1.955916979864579 1103 90-91 1.8710953278610631 1103 92-93 1.7901523863214344 1103 94-95 1.9957838405096489 1103 96-97 2.1968815274919393 1103 98-99 2.4219152878816743 1103 100-101 2.049954115889909 1104 1 0.4142470189278171 1104 2 0.2977483126264673 1104 3 0.39795082908336354 1104 4 0.20335095328966446 1104 5 0.23469360970752007 1104 6 0.39595418391475334 1104 7 0.3182393946833528 1104 8 0.39760669265118764 1104 9 0.5384931831964224 1104 10-11 0.6213185238617314 1104 12-13 0.6493619398978794 1104 14-15 0.7520791722569413 1104 16-17 0.7839127546158267 1104 18-19 0.8073014140063819 1104 20-21 0.7432312154830569 1104 22-23 0.8241498365111681 1104 24-25 0.7849889258166911 1104 26-27 0.8905765886967885 1104 28-29 0.7623712406354599 1104 30-31 0.7568533175546435 1104 32-33 0.9296998473583145 1104 34-35 0.7808787622784266 1104 36-37 0.9376639564339939 1104 38-39 0.995319315077225 1104 40-41 0.8435457510503639 1104 42-43 1.0120173387328393 1104 44-45 1.0296296000252383 1104 46-47 1.0580546218620057 1104 48-49 0.9300266119343945 1104 50-51 0.9631945058366895 1104 52-53 1.1854858649942628 1104 54-55 1.1406083954333113 1104 56-57 1.2055442862220431 1104 58-59 1.2186042911806751 1104 60-61 1.1467399730020702 1104 62-63 1.3180785423652388 1104 64-65 1.3733545708818191 1104 66-67 1.2536065677154973 1104 68-69 1.1871479561232867 1104 70-71 1.3536762900963772 1104 72-73 1.5028600635515197 1104 74-75 1.071374066350888 1104 76-77 1.1901031625257872 1104 78-79 1.3771343704859582 1104 80-81 1.4542449408901312 1104 82-83 1.3946733663612072 1104 84-85 1.366004230037209 1104 86-87 1.4978448119963588 1104 88-89 1.7651327152954117 1104 90-91 1.8568576390111744 1104 92-93 1.8551268465389157 1104 94-95 1.9320310071084172 1104 96-97 2.2787740480090726 1104 98-99 2.45903136419113 1104 100-101 1.9891199904228998 1105 1 0.3452991509866621 1105 2 0.26332691746681647 1105 3 0.2361663172988493 1105 4 0.21653650003225522 1105 5 0.20856995707379866 1105 6 0.35516937836484885 1105 7 0.3189444316300012 1105 8 0.14514923214003517 1105 9 0.46106792389196727 1105 10-11 0.42720626028637554 1105 12-13 0.49352052801619806 1105 14-15 0.5631422640247266 1105 16-17 0.3672381788808394 1105 18-19 0.4472382544465745 1105 20-21 0.4777548635973119 1105 22-23 0.5445913884963431 1105 24-25 0.566939302062373 1105 26-27 0.5810518107156284 1105 28-29 0.4764341290272043 1105 30-31 0.5895499708888821 1105 32-33 0.6691802215702296 1105 34-35 0.6973358386348281 1105 36-37 0.7230898032692323 1105 38-39 0.6136898210316275 1105 40-41 0.7251492581572307 1105 42-43 0.7738391361251047 1105 44-45 0.7828847465689748 1105 46-47 0.7741608745454371 1105 48-49 0.6081484341837324 1105 50-51 0.8390904902897276 1105 52-53 0.7964337103380856 1105 54-55 0.9440906452242857 1105 56-57 0.780150527606807 1105 58-59 0.8949070277183111 1105 60-61 0.877167853597733 1105 62-63 0.8016706152190558 1105 64-65 0.9863305273141485 1105 66-67 1.0073407778255508 1105 68-69 0.8398477730848519 1105 70-71 0.9933210599424918 1105 72-73 1.0705181884244084 1105 74-75 1.0089364914972023 1105 76-77 1.0437808792907504 1105 78-79 1.170095299688498 1105 80-81 1.2092768031367633 1105 82-83 1.2612095334007272 1105 84-85 1.2794213994946482 1105 86-87 1.310650158526542 1105 88-89 1.3146869828027015 1105 90-91 1.3537279076935214 1105 92-93 1.4727900558128617 1105 94-95 1.670140287835146 1105 96-97 1.7078918479322418 1105 98-99 1.762811333910701 1105 100-101 1.5329469626055747 1106 1 0.2369814099330938 1106 2 0.17327671455569416 1106 3 0.12236432348654347 1106 4 0.06801686298823029 1106 5 0.23544154974731413 1106 6 -9.223388469479232E-4 1106 7 0.2809357983904093 1106 8 0.15184241529529885 1106 9 0.028399117756876535 1106 10-11 -0.025665756897041092 1106 12-13 0.016338627826783636 1106 14-15 0.278839267769186 1106 16-17 0.047976157060105606 1106 18-19 -0.010461351728196178 1106 20-21 0.05857295648653604 1106 22-23 0.09575982895305657 1106 24-25 -0.07367176149644905 1106 26-27 -0.009684183341228447 1106 28-29 0.021761001658084922 1106 30-31 -0.2687086502383309 1106 32-33 0.02639141063632877 1106 34-35 -0.36984545016802883 1106 36-37 -0.12755469044501666 1106 38-39 -0.0627209246061824 1106 40-41 0.17641321334374993 1106 42-43 -0.3249014075705787 1106 44-45 -0.2079854064622353 1106 46-47 0.05312871561261545 1106 48-49 0.2388445958297254 1106 50-51 0.08557772846054945 1106 52-53 -0.11219716445226169 1106 54-55 -0.005971300922944067 1106 56-57 -0.8023079846243846 1106 58-59 -0.46497465167980323 1106 60-61 0.36234051838948034 1106 62-63 0.2507747608813311 1106 64-65 0.13061585103755746 1106 66-67 -0.3273595620130898 1106 68-69 -0.26169263264511144 1106 70-71 -0.36691931879417794 1106 72-73 -0.07778256069715539 1106 74-75 0.3453136454812551 1106 76-77 0.33743780145547575 1106 78-79 0.3699519122378412 1106 80-81 0.29048791376080985 1106 82-83 0.03498122320395325 1106 84-85 0.21961464378857087 1106 86-87 0.11091611167817916 1106 88-89 -0.0778365094373008 1106 90-91 0.09592338527870581 1106 92-93 0.18332147762113848 1106 94-95 0.23839040722856097 1106 96-97 0.23014793353558716 1106 98-99 0.4367686314436945 1106 100-101 0.12049273008027228 1107 1 0.28994681835202485 1107 2 0.11739062459897198 1107 3 0.18623131567388995 1107 4 0.14466858196510657 1107 5 0.03999787104037722 1107 6 0.20903045633454553 1107 7 0.1669169403709745 1107 8 0.08491296959022776 1107 9 0.17005580322182823 1107 10-11 0.31682920427653727 1107 12-13 0.36508853747367453 1107 14-15 0.4332971586422971 1107 16-17 0.552506995147013 1107 18-19 0.4437089844568476 1107 20-21 0.46260757029977384 1107 22-23 0.43802677073259133 1107 24-25 0.4741730977275438 1107 26-27 0.3831969752073263 1107 28-29 0.37888383707980466 1107 30-31 0.4144956601661107 1107 32-33 0.48924525266257746 1107 34-35 0.3958772467439218 1107 36-37 0.4478487312758972 1107 38-39 0.4452907004727251 1107 40-41 0.411342120114206 1107 42-43 0.5131060068023316 1107 44-45 0.5532381806861437 1107 46-47 0.519840118470384 1107 48-49 0.4662119951785968 1107 50-51 0.3156382368309565 1107 52-53 0.37178193149832595 1107 54-55 0.5422122358086838 1107 56-57 0.6278532276567574 1107 58-59 0.4519449012681136 1107 60-61 0.42123571582735053 1107 62-63 0.5154028306385037 1107 64-65 0.6370859153086421 1107 66-67 0.6563101827850524 1107 68-69 0.7156778110587325 1107 70-71 0.8504143893206653 1107 72-73 0.9104801382011658 1107 74-75 0.984866262716011 1107 76-77 1.006520997029419 1107 78-79 0.832936186720012 1107 80-81 0.8623521750779624 1107 82-83 0.5787884857054486 1107 84-85 0.8250454602354118 1107 86-87 0.8559570250159503 1107 88-89 1.02785204377685 1107 90-91 1.0680553599098985 1107 92-93 1.172354370396274 1107 94-95 1.0916028385197052 1107 96-97 1.1497523282658726 1107 98-99 1.1682865200176238 1107 100-101 1.0565612757712373 1108 1 0.2062809950527047 1108 2 0.06641222447219874 1108 3 0.04821865889664778 1108 4 0.13195517734843065 1108 5 0.21217294855049573 1108 6 0.21095265345868341 1108 7 0.2424588620846393 1108 8 0.17334131156762567 1108 9 0.2802287203865035 1108 10-11 0.3921174219333565 1108 12-13 0.25455783827559486 1108 14-15 0.4236050369646165 1108 16-17 0.497327202121177 1108 18-19 0.42442339135563145 1108 20-21 0.39092022680843996 1108 22-23 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2316 72-73 -2.175724287420895 2316 74-75 -2.2754235114168395 2316 76-77 -2.3910403622744525 2316 78-79 -2.6751220243077505 2316 80-81 -2.7933089562906304 2316 82-83 -2.8597062507049777 2316 84-85 -2.9034540341971713 2316 86-87 -2.853936684561255 2316 88-89 -3.1440184983334127 2316 90-91 -3.3072812624411654 2316 92-93 -3.1276411317514494 2316 94-95 -3.229754761020633 2316 96-97 -3.461455360503919 2316 98-99 -3.533296139703536 2316 100-101 -3.2327693004962423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9953.0 3 3580.0 4 3724.0 5 4158.0 6 5058.0 7 6270.0 8 7802.0 9 9532.0 10 12395.0 11 15435.0 12 18277.0 13 21292.0 14 24483.0 15 27471.0 16 31404.0 17 35967.0 18 35206.0 19 34615.0 20 36898.0 21 40434.0 22 44190.0 23 49870.0 24 55456.0 25 61962.0 26 69617.0 27 78259.0 28 88995.0 29 101394.0 30 116848.0 31 135638.0 32 159432.0 33 189916.0 34 228862.0 35 278135.0 36 330200.0 37 326404.0 38 147939.0 39 6538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02895112198301 18.78122119694543 14.498270502518022 23.69155717855353 2 15.326428316835575 19.841444692978886 40.42146467552223 24.410662314663316 3 19.25084195461883 23.69310644502709 29.366085973223754 27.689965627130324 4 12.899709276826984 16.358494021684596 36.86296854814546 33.878828153342965 5 14.115877158867063 37.048131450626684 34.14281341744391 14.693177973062339 6 32.70847305901732 35.73391412447769 17.43962447348556 14.117988343019421 7 29.213236598270235 31.8902175277036 20.51353054704758 18.38301532697858 8 26.331976443181144 35.31342550091189 19.679674374405636 18.67492368150132 9 26.26669363282122 16.82547838927934 19.942172320036846 36.96565565786259 10-11 24.95830342902362 26.521824039593948 27.5146529656719 21.005219565710533 12-13 25.854370646013024 23.592721109389352 27.81718308076403 22.735725163833592 14-15 22.63585004800491 25.242294238744435 25.84104993176958 26.280805781481074 16-17 22.558388830737066 28.39190853812863 26.519725619994627 22.529977011139675 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28.119772481571793 22.779010141999205 44-45 22.540202110117946 26.777838329428626 28.082004068837296 22.59995549161613 46-47 22.64397469952443 26.948469224495632 27.65242653318506 22.755129542794876 48-49 22.602519245330367 26.91651177587643 27.66878423273379 22.81218474605942 50-51 22.762982639580688 26.981292396311225 27.5858256156736 22.669899348434484 52-53 22.865622220337475 26.933103051399183 27.57118564588405 22.630089082379296 54-55 22.512199062626937 26.958552801973017 27.722196774043184 22.807051361356862 56-57 22.588533287022862 27.16252749319056 27.57443248499539 22.674506734791187 58-59 22.657722642002778 26.76728431539551 27.6669617865337 22.908031256068007 60-61 22.53102036173758 26.9007294094183 27.81084813145248 22.75740209739164 62-63 22.468152576041987 26.9679023387722 27.88244245594212 22.681502629243695 64-65 22.343455746560757 26.804380598344864 27.770576701913484 23.081586953180896 66-67 22.256497981217375 26.948386770047684 27.68302002380728 23.11209522492766 68-69 22.529230765995635 26.75404310082426 27.764702212694026 22.952023920486077 70-71 22.436304038399417 26.826574273994773 27.565404838101042 23.17171684950477 72-73 22.489084079186675 26.799402768165 27.583273933221015 23.128239219427314 74-75 22.459853589866547 27.040445764313613 27.371891133227333 23.127809512592506 76-77 22.493815628168313 26.800679645595842 27.544574358363207 23.160930367872638 78-79 22.62208175271553 26.917991979173255 27.400899228354202 23.05902703975701 80-81 22.60227597692062 27.13131024273723 27.23022538283401 23.036188397508145 82-83 22.65650065771709 27.028641489603476 27.247115358162745 23.06774249451669 84-85 22.659909485263142 26.934459872898074 27.272908870570372 23.132721771268404 86-87 22.75816180492068 26.89918441970185 27.210444110205067 23.132209665172404 88-89 22.761088533865724 26.853090346277586 27.119588508888093 23.266232610968597 90-91 22.68602741243503 26.92933367524238 27.181961925751867 23.202676986570722 92-93 22.8138692783881 26.631075652521403 27.267086841607135 23.287968227483365 94-95 22.704463263664046 26.562282520856083 27.255971966335764 23.477282249144107 96-97 22.893321280369708 26.440356323187004 27.143743861551485 23.522578534891807 98-99 22.808032825614973 26.48417711979485 26.894216889937024 23.813573164653157 100-101 23.33996618577902 26.02828793500038 27.17608469757454 23.455661181646054 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 328.0 1 245.5 2 220.5 3 281.0 4 239.0 5 235.5 6 441.5 7 1021.0 8 1639.0 9 1863.0 10 1686.0 11 1502.0 12 1563.0 13 1680.0 14 1887.5 15 2096.5 16 2231.0 17 2463.5 18 2755.5 19 3061.5 20 3570.0 21 4216.5 22 4983.0 23 6010.5 24 7361.0 25 9309.5 26 12078.5 27 15595.5 28 19104.0 29 23332.0 30 28529.0 31 34342.5 32 41578.0 33 46927.0 34 51193.5 35 57163.5 36 63582.0 37 69464.5 38 74191.5 39 78788.5 40 84310.0 41 90918.0 42 96784.0 43 101776.0 44 106327.5 45 111173.0 46 113733.0 47 115129.0 48 117269.0 49 116686.5 50 113898.5 51 110365.5 52 106541.5 53 103006.5 54 99562.5 55 91918.0 56 81745.0 57 73396.0 58 65844.5 59 56774.5 60 46688.0 61 38678.0 62 31765.5 63 24300.5 64 18396.5 65 14187.5 66 10483.0 67 7938.0 68 5999.0 69 4447.0 70 3490.5 71 2710.0 72 2192.5 73 1810.0 74 1406.0 75 1134.5 76 842.0 77 507.0 78 306.5 79 201.0 80 117.0 81 79.5 82 52.0 83 33.0 84 24.5 85 17.5 86 13.0 87 6.5 88 5.5 89 4.0 90 4.5 91 5.0 92 4.5 93 2.0 94 0.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006272758461302863 2 0.10937027462416891 3 0.11900719404795823 4 0.12177561817333771 5 0.16333702339738906 6 0.09759571125546633 7 0.025616683995599954 8 0.026703027639736206 9 0.022778173183502014 10-11 0.05102310793104451 12-13 0.05443983390856982 14-15 0.027193634446765483 16-17 0.032345005920572864 18-19 0.038039549216448365 20-21 0.059065555231988684 22-23 0.03180183409850474 24-25 0.05009445933202481 26-27 0.058136906632968985 28-29 0.05407187880329786 30-31 0.06875503967081685 32-33 0.05102310793104451 34-35 0.06307801804662096 36-37 0.05305562184588008 38-39 0.030820620484446188 40-41 0.03588438359985548 42-43 0.03213474586041746 44-45 0.007499275478876048 46-47 0.013386557163227338 48-49 0.00891853088492502 50-51 0.008778357511488085 52-53 0.010337786290973991 54-55 0.011073696501517901 56-57 0.01410494570209163 58-59 0.018608015323753183 60-61 0.023987168529395583 62-63 0.011108739844877136 64-65 0.00891853088492502 66-67 0.0098296578122651 68-69 0.011301478233352922 70-71 0.030049666930543045 72-73 0.027351329491882036 74-75 0.02806971803074633 76-77 0.022830738198540868 78-79 0.005694543295875504 80-81 0.005361631533962782 82-83 0.006255236789623246 84-85 0.005291544847244314 86-87 0.012773298654440745 88-89 0.03702329225903058 90-91 0.03499077834419502 92-93 0.029050931644804875 94-95 0.04639738660762564 96-97 0.04199944701604179 98-99 0.025055990501852216 100-101 0.07520301484891588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2853609.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.18813204488787 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56335149838618 40.40448736703938 2 13.511812199666753 14.643597272825376 3 4.925973754058717 8.007879487037572 4 2.3976029582496774 5.196865027713892 5 1.268037995972693 3.4356305181851634 6 0.7849936636601775 2.5522404180490796 7 0.4918229714522766 1.8655677683835556 8 0.36215438671369665 1.569957578230174 9 0.2687717113453596 1.310781328588155 >10 1.3062497676784237 12.518129474717474 >50 0.07894757420107847 2.925927708945949 >100 0.03697471929979706 3.6073053491015314 >500 0.002399050483632837 0.8957707139230627 >1k 7.780704270338316E-4 0.6430435616701234 >5k 1.296784045056386E-4 0.4228164255894583 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6708 0.23507074725374077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5326 0.1866408467312796 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3741 0.1310971475068939 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.504334335923387E-5 3 0.0 0.0 0.0 0.0 3.504334335923387E-5 4 0.0 0.0 0.0 0.0 3.504334335923387E-5 5 3.504334335923387E-5 0.0 0.0 0.0 3.504334335923387E-5 6 3.504334335923387E-5 0.0 0.0 0.0 3.504334335923387E-5 7 3.504334335923387E-5 0.0 0.0 0.0 3.504334335923387E-5 8 3.504334335923387E-5 0.0 0.0 0.0 3.504334335923387E-5 9 3.504334335923387E-5 0.0 0.0 0.0 3.504334335923387E-5 10-11 3.504334335923387E-5 0.0 0.0 0.0 3.504334335923387E-5 12-13 3.504334335923387E-5 0.0 0.0 0.0 1.051300300777016E-4 14-15 3.504334335923387E-5 0.0 0.0 0.0 1.576950451165524E-4 16-17 3.504334335923387E-5 0.0 0.0 1.7521671679616935E-5 1.7521671679616933E-4 18-19 3.504334335923387E-5 0.0 0.0 7.008668671846774E-5 1.7521671679616933E-4 20-21 3.504334335923387E-5 0.0 0.0 1.576950451165524E-4 1.9273838847578629E-4 22-23 3.504334335923387E-5 0.0 0.0 3.504334335923387E-4 2.4530340351463706E-4 24-25 3.504334335923387E-5 0.0 0.0 7.884752255827621E-4 2.4530340351463706E-4 26-27 3.504334335923387E-5 0.0 0.0 0.0011389086591751007 2.4530340351463706E-4 28-29 3.504334335923387E-5 0.0 0.0 0.003311595947447601 2.4530340351463706E-4 30-31 3.504334335923387E-5 0.0 0.0 0.010215134589216673 2.4530340351463706E-4 32-33 7.008668671846774E-5 0.0 0.0 0.02498590381513375 2.4530340351463706E-4 34-35 1.051300300777016E-4 0.0 0.0 0.0491482890613255 3.153900902331048E-4 36-37 1.051300300777016E-4 0.0 0.0 0.09158577786935772 3.153900902331048E-4 38-39 1.051300300777016E-4 0.0 0.0 0.15398045072047362 3.5043343359233867E-4 40-41 1.051300300777016E-4 3.504334335923387E-5 0.0 0.22170171176219305 3.5043343359233867E-4 42-43 1.051300300777016E-4 3.504334335923387E-5 0.0 0.2969397699544682 3.5043343359233867E-4 44-45 1.051300300777016E-4 3.504334335923387E-5 0.0 0.3897695865130787 3.6795510527195565E-4 46-47 1.051300300777016E-4 3.504334335923387E-5 0.0 0.500997158335287 4.029984486311895E-4 48-49 1.051300300777016E-4 3.504334335923387E-5 0.0 0.6207928276088279 4.906068070292741E-4 50-51 1.051300300777016E-4 3.504334335923387E-5 0.0 0.7463356051932833 4.906068070292741E-4 52-53 1.4017337343693546E-4 3.504334335923387E-5 0.0 0.870581779073447 5.606934937477419E-4 54-55 1.7521671679616933E-4 3.504334335923387E-5 0.0 1.0099841989564793 5.957368371069758E-4 56-57 1.7521671679616933E-4 3.504334335923387E-5 0.0 1.176405036569481 5.957368371069758E-4 58-59 1.7521671679616933E-4 3.504334335923387E-5 0.0 1.35530130441837 5.957368371069758E-4 60-61 1.9273838847578629E-4 3.504334335923387E-5 0.0 1.5508081170195356 5.957368371069758E-4 62-63 2.102600601554032E-4 3.504334335923387E-5 0.0 1.7453512376783222 5.957368371069758E-4 64-65 2.102600601554032E-4 3.504334335923387E-5 0.0 1.9537364789640068 6.132585087865927E-4 66-67 2.102600601554032E-4 7.008668671846774E-5 0.0 2.19464194288706 6.307801804662096E-4 68-69 2.102600601554032E-4 7.008668671846774E-5 0.0 2.4597623570713436 6.658235238254435E-4 70-71 2.102600601554032E-4 7.008668671846774E-5 0.0 2.7389001085993208 6.658235238254435E-4 72-73 2.102600601554032E-4 7.008668671846774E-5 0.0 3.02709656438566 6.658235238254435E-4 74-75 2.102600601554032E-4 7.008668671846774E-5 0.0 3.319568308061826 6.658235238254435E-4 76-77 2.102600601554032E-4 7.008668671846774E-5 0.0 3.637604170718553 6.658235238254435E-4 78-79 2.102600601554032E-4 7.008668671846774E-5 0.0 3.989001296253271 6.658235238254435E-4 80-81 2.4530340351463706E-4 7.008668671846774E-5 0.0 4.368748486565609 6.833451955050604E-4 82-83 2.4530340351463706E-4 7.008668671846774E-5 0.0 4.753541918321677 7.183885388642943E-4 84-85 2.4530340351463706E-4 7.008668671846774E-5 0.0 5.1425405512808515 7.709535539031451E-4 86-87 2.4530340351463706E-4 7.008668671846774E-5 0.0 5.546152258420827 7.709535539031451E-4 88-89 2.4530340351463706E-4 7.008668671846774E-5 0.0 5.991237762426458 7.709535539031451E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3785 0.0 55.29176 1 GTATCAA 7020 0.0 41.804367 1 ATCAACG 9105 0.0 32.021854 3 TCAACGC 9285 0.0 31.401077 4 CAACGCA 9370 0.0 31.014702 5 TATCAAC 9445 0.0 30.768421 2 AACGCAG 9815 0.0 29.682522 6 GTACATG 8975 0.0 26.28578 1 TACATGG 8935 0.0 25.285122 2 ACGCAGA 11450 0.0 25.026745 7 CGCAGAG 11610 0.0 24.558617 8 ACATGGG 9410 0.0 23.149511 3 TGGTATC 1975 0.0 21.914938 2 GCAGAGT 12700 0.0 21.664293 9 GTGGTAT 2335 0.0 20.980892 1 GGACCGA 720 0.0 20.462551 6 GAGTACT 7225 0.0 20.223396 12-13 AGAGTAC 11700 0.0 20.063513 10-11 CATGGGG 5645 0.0 19.547447 4 TAAGGTG 1200 0.0 19.421412 5 >>END_MODULE