FastQCFastQC Report
Thu 26 May 2016
SRR938411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853609
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67730.23734856457209097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55760.19540168257108806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41900.1468316086751899No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.052.96571
GTATCAA76600.041.8283921
ATCAACG95050.033.1380773
TCAACGC96400.032.6740074
TATCAAC98100.032.2244152
CAACGCA97850.032.1412735
AACGCAG101200.031.0626036
ACGCAGA117900.026.4613467
CGCAGAG119000.026.1369388
GTACATG94000.023.6172371
TACATGG91900.023.5357672
GTGGTAT22850.022.8847681
GCAGAGT136650.022.517799
ACATGGG94350.022.2056053
TGGTATC22150.021.2468192
TAGACCG1404.8487188E-420.360534
GAGTACT75350.020.355412-13
AGAGTAC121250.020.11032510-11
CAGAGTA128200.019.52014210-11
AGTACTT82750.018.70724912-13
Produced by FastQC (version 0.11.5)