##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938411_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2853609 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.814849897095222 31.0 31.0 34.0 30.0 34.0 2 32.04519785296444 31.0 31.0 34.0 30.0 34.0 3 32.11175812804067 33.0 31.0 34.0 30.0 34.0 4 35.77267207946148 37.0 35.0 37.0 35.0 37.0 5 35.67027052409773 37.0 35.0 37.0 33.0 37.0 6 35.665812660389 37.0 35.0 37.0 33.0 37.0 7 35.60725628493602 37.0 35.0 37.0 33.0 37.0 8 35.58469187614701 37.0 35.0 37.0 33.0 37.0 9 37.177149006749 39.0 37.0 39.0 33.0 39.0 10-11 37.08197759398712 39.0 37.0 39.0 33.0 39.0 12-13 36.969736218241536 39.0 37.0 39.0 33.0 39.0 14-15 38.02898627667631 40.0 37.0 41.0 33.0 41.0 16-17 37.94001665960543 40.0 37.0 41.0 33.0 41.0 18-19 38.177807646387436 40.0 37.5 41.0 33.0 41.0 20-21 38.14891843977223 40.0 37.0 41.0 33.0 41.0 22-23 38.03853050645691 40.0 37.0 41.0 33.0 41.0 24-25 37.92218537998724 40.0 37.0 41.0 32.5 41.0 26-27 37.73729302087287 40.0 37.0 41.0 32.0 41.0 28-29 37.63261277210718 40.0 37.0 41.0 32.0 41.0 30-31 37.401195468615356 39.0 36.0 41.0 31.5 41.0 32-33 37.22655171048311 39.0 36.0 41.0 31.0 41.0 34-35 37.14243647255107 39.0 36.0 41.0 31.0 41.0 36-37 37.11171730254566 39.0 36.0 41.0 31.0 41.0 38-39 36.96589967301057 39.0 36.0 41.0 31.0 41.0 40-41 36.80848444899074 39.0 35.5 41.0 30.0 41.0 42-43 36.61947029183045 39.0 35.0 40.5 30.0 41.0 44-45 36.34607158864441 39.0 35.0 40.0 30.0 41.0 46-47 36.001449743114776 38.5 35.0 40.0 29.5 41.0 48-49 35.68446815944301 38.0 35.0 40.0 28.0 41.0 50-51 35.33394203620749 38.0 34.0 40.0 27.0 41.0 52-53 34.866650792032125 38.0 34.0 40.0 26.0 41.0 54-55 34.54375792198581 38.0 33.0 40.0 25.5 41.0 56-57 34.224546530376095 37.5 33.0 40.0 25.0 41.0 58-59 33.75678500453286 37.0 32.5 40.0 23.0 41.0 60-61 33.370677096967384 36.5 32.0 39.0 22.5 40.5 62-63 32.89098121010973 36.0 31.0 39.0 22.0 40.0 64-65 32.366672518905006 35.5 31.0 39.0 20.0 40.0 66-67 31.85964632856148 35.0 30.5 38.0 18.5 40.0 68-69 31.355623703177272 35.0 30.0 38.0 17.5 40.0 70-71 30.770768174616776 34.0 29.0 37.0 13.0 39.0 72-73 30.24914958566503 34.0 29.0 36.5 9.5 39.0 74-75 29.64182303882557 34.0 28.0 36.0 7.5 39.0 76-77 28.405880763622484 31.5 26.5 34.5 7.0 37.0 78-79 29.092092154180897 33.5 28.0 35.0 4.5 37.0 80-81 28.696010385445238 33.0 27.5 35.0 2.0 37.0 82-83 28.235714843904685 33.0 26.5 35.0 2.0 36.0 84-85 27.655783080302875 33.0 26.0 35.0 2.0 36.0 86-87 27.027698258591137 32.0 25.5 35.0 2.0 35.5 88-89 26.270710177883515 32.0 24.5 34.0 2.0 35.0 90-91 25.637169282827465 31.5 22.0 34.0 2.0 35.0 92-93 24.788056107196184 31.0 19.0 34.0 2.0 35.0 94-95 23.948985302471364 31.0 12.0 34.0 2.0 35.0 96-97 22.861051741846904 30.0 2.0 34.0 2.0 35.0 98-99 21.46718769109573 29.5 2.0 34.0 2.0 35.0 100-101 17.46909107029029 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2651775064686568 1101 2 0.2917796415767526 1101 3 0.30491035990316817 1101 4 0.24636075248973555 1101 5 0.07200022445758236 1101 6 0.16048522080412653 1101 7 0.1809321923650984 1101 8 0.18832243370930968 1101 9 0.25151910876074624 1101 10-11 0.26434315082268967 1101 12-13 0.40717189050076996 1101 14-15 0.4315817617224056 1101 16-17 0.4960191044752875 1101 18-19 0.4468560958768464 1101 20-21 0.3784050288077907 1101 22-23 0.3780920498805571 1101 24-25 0.4917385184847305 1101 26-27 0.49289464085559587 1101 28-29 0.44174909748844726 1101 30-31 0.553406774294217 1101 32-33 0.3728055660211069 1101 34-35 0.2931165238745237 1101 36-37 0.45216196391851327 1101 38-39 0.5668041527637655 1101 40-41 0.5955212331910502 1101 42-43 0.5247631628992124 1101 44-45 0.5377818115176112 1101 46-47 0.38313104518404373 1101 48-49 0.406198217990287 1101 50-51 0.5256351058600686 1101 52-53 0.478051189109685 1101 54-55 0.5839630229181765 1101 56-57 0.4469197169040413 1101 58-59 0.7449464062409774 1101 60-61 0.6870028615064712 1101 62-63 0.8087880747680529 1101 64-65 0.5779454266942068 1101 66-67 0.5262429621950169 1101 68-69 0.7962379128149699 1101 70-71 0.8905930251914356 1101 72-73 0.7858489427675757 1101 74-75 0.6339339843474967 1101 76-77 0.581052783414723 1101 78-79 0.8254591458318821 1101 80-81 0.8451200921266633 1101 82-83 0.7758024236601706 1101 84-85 0.9779401791099396 1101 86-87 1.0740595687096466 1101 88-89 0.8962230217239977 1101 90-91 1.0917707912797638 1101 92-93 1.408577822082588 1101 94-95 1.1041012822673082 1101 96-97 1.0947010468935865 1101 98-99 1.0345294613958984 1101 100-101 1.2075312854559321 1102 1 0.27178011036725636 1102 2 0.3020472705241062 1102 3 0.2872212131528329 1102 4 0.22767544157154873 1102 5 0.12473034596055754 1102 6 0.18693998054853012 1102 7 0.2033488550521625 1102 8 0.283385805416124 1102 9 0.27452841648413084 1102 10-11 0.4030177938775452 1102 12-13 0.3962793375132989 1102 14-15 0.48456165070284385 1102 16-17 0.4613231550038748 1102 18-19 0.44005653320365923 1102 20-21 0.3758527766176769 1102 22-23 0.4578254215191535 1102 24-25 0.5421768938868894 1102 26-27 0.4892969532198279 1102 28-29 0.4688097044448867 1102 30-31 0.41485947854198457 1102 32-33 0.4408527380898022 1102 34-35 0.4554763123466046 1102 36-37 0.5883515658596465 1102 38-39 0.5792486878336689 1102 40-41 0.493625893470643 1102 42-43 0.41550555724580107 1102 44-45 0.6037945219912402 1102 46-47 0.5323017337766913 1102 48-49 0.5702124475353116 1102 50-51 0.6927931587703497 1102 52-53 0.5553103845829952 1102 54-55 0.5295201559968703 1102 56-57 0.6183306391082297 1102 58-59 0.7405722538017017 1102 60-61 0.6406502120635338 1102 62-63 0.7810655903131405 1102 64-65 0.7425136422729821 1102 66-67 0.6328395398373239 1102 68-69 0.8239465634111092 1102 70-71 0.8857121366104685 1102 72-73 0.9315340042972942 1102 74-75 1.1486259845364977 1102 76-77 1.0695897574659448 1102 78-79 1.1625303372694944 1102 80-81 1.1763256642786395 1102 82-83 1.1816420428715233 1102 84-85 1.1419893407275659 1102 86-87 1.1430205966449414 1102 88-89 1.112521720381114 1102 90-91 1.2516684687327988 1102 92-93 1.7584368683270917 1102 94-95 1.7258518730804617 1102 96-97 1.5126787085596014 1102 98-99 1.5968196004950315 1102 100-101 1.6765071832101945 1103 1 0.22461850814555007 1103 2 0.257784919211673 1103 3 0.22523932532158852 1103 4 0.21584940246450657 1103 5 0.19695472920075474 1103 6 0.15992243105533532 1103 7 0.23486744414501004 1103 8 0.20927598467195452 1103 9 0.2260569454487822 1103 10-11 0.2618665056716196 1103 12-13 0.2896312150568008 1103 14-15 0.5205221656203136 1103 16-17 0.45139228406335263 1103 18-19 0.4722329150854563 1103 20-21 0.34298685566034237 1103 22-23 0.3690005887709944 1103 24-25 0.42661189634510066 1103 26-27 0.40498003235176583 1103 28-29 0.40341071200634104 1103 30-31 0.34930181548709527 1103 32-33 0.3135352895765635 1103 34-35 0.3339374786103022 1103 36-37 0.4300991247405008 1103 38-39 0.31548983505932426 1103 40-41 0.3791550481168997 1103 42-43 0.5453894071921823 1103 44-45 0.4176730375884006 1103 46-47 0.4621363587338436 1103 48-49 0.6318030410984292 1103 50-51 0.5874296054048429 1103 52-53 0.4984510216056819 1103 54-55 0.5030410770430009 1103 56-57 0.6687951322854602 1103 58-59 0.674783000977925 1103 60-61 0.7868906367874899 1103 62-63 0.863747455788193 1103 64-65 0.6808302425157109 1103 66-67 0.7339655112919452 1103 68-69 0.6231759922431088 1103 70-71 0.5718013273769493 1103 72-73 0.6134577416890927 1103 74-75 0.6232085816843913 1103 76-77 0.7037185481367665 1103 78-79 0.8738113011500026 1103 80-81 0.7979895978454579 1103 82-83 0.8864341144249757 1103 84-85 1.0317051129395658 1103 86-87 1.085145230863258 1103 88-89 1.0039569006671805 1103 90-91 1.0823098142693546 1103 92-93 1.4448105593401195 1103 94-95 1.3479906629801341 1103 96-97 1.6390235165334879 1103 98-99 1.8286565282134788 1103 100-101 2.1960120530419083 1104 1 0.18935410959263166 1104 2 0.19985574814909768 1104 3 0.2170463998587593 1104 4 0.14766741018737406 1104 5 0.038686420467087146 1104 6 0.046378163839925435 1104 7 0.18188153541970564 1104 8 0.20503014816138432 1104 9 0.2172404105722734 1104 10-11 0.24748100990039745 1104 12-13 0.25957888200549917 1104 14-15 0.3840878465593178 1104 16-17 0.45242780298925567 1104 18-19 0.3551891579693063 1104 20-21 0.3604510529590783 1104 22-23 0.46275735672632123 1104 24-25 0.4595368020724848 1104 26-27 0.45775737489125845 1104 28-29 0.3995637278594657 1104 30-31 0.3628399893665275 1104 32-33 0.348248502366296 1104 34-35 0.4957575058388102 1104 36-37 0.4344454142470795 1104 38-39 0.40671055657993804 1104 40-41 0.4425234790654784 1104 42-43 0.5515342753846184 1104 44-45 0.4464593495184559 1104 46-47 0.4177594632711106 1104 48-49 0.4922045397108903 1104 50-51 0.57060503428864 1104 52-53 0.4431008985202993 1104 54-55 0.5374981956262843 1104 56-57 0.49125168679992015 1104 58-59 0.6706329168898932 1104 60-61 0.6648806367526703 1104 62-63 0.6668762967660484 1104 64-65 0.6441553237311766 1104 66-67 0.6160866189559648 1104 68-69 0.6171310547607369 1104 70-71 0.756365862768785 1104 72-73 0.760869466660278 1104 74-75 0.7757199065845448 1104 76-77 0.7153012834526997 1104 78-79 0.961793849835761 1104 80-81 0.8169372613122547 1104 82-83 1.0652535044646392 1104 84-85 1.261014929477927 1104 86-87 1.4683815931068587 1104 88-89 1.5425999669731247 1104 90-91 1.555904429849491 1104 92-93 1.8810718276593015 1104 94-95 1.606924538098081 1104 96-97 1.4058747242326781 1104 98-99 1.6656648395237568 1104 100-101 1.7552667858230109 1105 1 0.11719177031619665 1105 2 0.13258347282313565 1105 3 0.13204651284544866 1105 4 0.1066453325881156 1105 5 0.07824306069486653 1105 6 0.09598948962574383 1105 7 0.12385157134946922 1105 8 0.06346234484860958 1105 9 0.09341603708346469 1105 10-11 0.14098201548193856 1105 12-13 0.21267528224955612 1105 14-15 0.31403892332247807 1105 16-17 0.25681959563608103 1105 18-19 0.24684396103350537 1105 20-21 0.1911150757371871 1105 22-23 0.13658229514857823 1105 24-25 0.2862688880327653 1105 26-27 0.17620923126257537 1105 28-29 0.10211892185760973 1105 30-31 0.16396703160094717 1105 32-33 0.28507142962546794 1105 34-35 0.3092187401054147 1105 36-37 0.23392059107124652 1105 38-39 0.20844829187472413 1105 40-41 0.16616085958204962 1105 42-43 0.2183918895576653 1105 44-45 0.2926104579178883 1105 46-47 0.19483672356984982 1105 48-49 0.38217753589193393 1105 50-51 0.48849930134398534 1105 52-53 0.5428664511382024 1105 54-55 0.40613986963708015 1105 56-57 0.29399443870374853 1105 58-59 0.2995411265028025 1105 60-61 0.3102312344522602 1105 62-63 0.44903400358818146 1105 64-65 0.43938954513989614 1105 66-67 0.18528870695000066 1105 68-69 0.33179955181178755 1105 70-71 0.3587329339278007 1105 72-73 0.2651414934155234 1105 74-75 0.40908657099148016 1105 76-77 0.2583492124066673 1105 78-79 0.44964947544306355 1105 80-81 0.39032833084426244 1105 82-83 0.5926951468391657 1105 84-85 0.6907750587346975 1105 86-87 0.6277176833154279 1105 88-89 0.6656961412437958 1105 90-91 0.7696481308415173 1105 92-93 0.8381865995391742 1105 94-95 0.9921165024846772 1105 96-97 1.1937156615702627 1105 98-99 1.4188776160586798 1105 100-101 1.302903104264697 1106 1 -0.04534269227502108 1106 2 0.11084764257172708 1106 3 0.07133582788486592 1106 4 0.012019189699003618 1106 5 -0.0979793086702827 1106 6 -0.07749075336003841 1106 7 0.07062979491392696 1106 8 0.08506436219353475 1106 9 -0.29552017338116343 1106 10-11 0.04037847313130527 1106 12-13 -0.03541471154429843 1106 14-15 -0.0538979469782177 1106 16-17 0.03230610937151113 1106 18-19 -0.0044708661286705365 1106 20-21 0.11818202371857467 1106 22-23 0.04268525956587865 1106 24-25 0.08360491531213654 1106 26-27 0.11959379802021886 1106 28-29 0.10628482909758219 1106 30-31 0.115027210494965 1106 32-33 0.07244335567768445 1106 34-35 0.1488863073175608 1106 36-37 0.09117976821415397 1106 38-39 0.02404003331128024 1106 40-41 0.09983516588901864 1106 42-43 0.10043846567759829 1106 44-45 0.042064420510257605 1106 46-47 -0.6287205771931212 1106 48-49 -0.20409285615681227 1106 50-51 0.14983088972625325 1106 52-53 0.19301900938719285 1106 54-55 0.34233891402836747 1106 56-57 0.16887020034143774 1106 58-59 -0.009993908253946415 1106 60-61 0.17232884404618432 1106 62-63 -0.1101648839189977 1106 64-65 0.06413893433011708 1106 66-67 -0.06193179134157489 1106 68-69 -0.10796139604501676 1106 70-71 -0.029223212352778916 1106 72-73 0.25094959647914905 1106 74-75 0.2813269105071221 1106 76-77 0.21733797904387941 1106 78-79 0.32023676413979274 1106 80-81 -0.3476219899606434 1106 82-83 0.05448204517134769 1106 84-85 0.21729556556261898 1106 86-87 0.3314233779774476 1106 88-89 0.5508013228025312 1106 90-91 0.4575775145407981 1106 92-93 0.5046427031380496 1106 94-95 0.7546833800989319 1106 96-97 0.888747684383052 1106 98-99 0.8101124669380368 1106 100-101 0.991419072989391 1107 1 0.08065461327532475 1107 2 0.11033876048456648 1107 3 0.0768753250110521 1107 4 0.046942689248155034 1107 5 -0.04880040751372405 1107 6 0.0010114865590438171 1107 7 0.0707851966665558 1107 8 0.10778251201222133 1107 9 0.06977080606659314 1107 10-11 0.12088792051887509 1107 12-13 0.14329088011464108 1107 14-15 0.22616455558435433 1107 16-17 0.2961511375153165 1107 18-19 0.16313685069113149 1107 20-21 0.2132957166909648 1107 22-23 0.1452344404221222 1107 24-25 0.18684563955357447 1107 26-27 0.2295987438401994 1107 28-29 0.25457670017825507 1107 30-31 0.18319646277792856 1107 32-33 0.2620560612177343 1107 34-35 0.3966918301237996 1107 36-37 0.33352422094694845 1107 38-39 0.3402852811848902 1107 40-41 0.3619584856145579 1107 42-43 0.4051650474099304 1107 44-45 0.3690216642484714 1107 46-47 0.38538556723131023 1107 48-49 0.5368515843091828 1107 50-51 0.5052253135628035 1107 52-53 0.4893389260712908 1107 54-55 0.42792992052339685 1107 56-57 0.43359987893686736 1107 58-59 0.39371292069787955 1107 60-61 0.4007269670253919 1107 62-63 0.5741985716216362 1107 64-65 0.4214833652776093 1107 66-67 0.34776610474660075 1107 68-69 0.6667799650531343 1107 70-71 0.7093156290919644 1107 72-73 0.6835596990677679 1107 74-75 0.5594311387195674 1107 76-77 0.49234914260975415 1107 78-79 0.8134722632626286 1107 80-81 0.8069754943631615 1107 82-83 0.9781570497211227 1107 84-85 0.9744798243370347 1107 86-87 1.0946703719143223 1107 88-89 1.213560192125744 1107 90-91 1.330565349521791 1107 92-93 1.3652322489012967 1107 94-95 1.3206980111013245 1107 96-97 1.3559059650341503 1107 98-99 1.308577609297103 1107 100-101 1.313158124734823 1108 1 0.09751144047723415 1108 2 0.0890813373746866 1108 3 0.16497263187589795 1108 4 0.1659257511729848 1108 5 0.09274978134862977 1108 6 0.11257804140343808 1108 7 0.10245086507994472 1108 8 0.10239776371081888 1108 9 0.11337600898059463 1108 10-11 0.12841152719455096 1108 12-13 0.20620422635811764 1108 14-15 0.29921257872950235 1108 16-17 0.3772977142227987 1108 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2316 72-73 -1.2577558411441352 2316 74-75 -1.1052339101901651 2316 76-77 -1.3038039479659638 2316 78-79 -1.5582134369625713 2316 80-81 -1.2508550900621884 2316 82-83 -1.4253867287129083 2316 84-85 -1.3977392792762835 2316 86-87 -1.7463766862036643 2316 88-89 -1.901436165495845 2316 90-91 -1.8969462159115977 2316 92-93 -2.1041874818537423 2316 94-95 -2.0720505360262464 2316 96-97 -2.203007631664601 2316 98-99 -2.0744514979806397 2316 100-101 -2.1624753117698337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 15.0 4 37.0 5 90.0 6 169.0 7 468.0 8 1078.0 9 1908.0 10 3200.0 11 4962.0 12 7193.0 13 10210.0 14 13414.0 15 17683.0 16 21829.0 17 27010.0 18 26745.0 19 23972.0 20 24495.0 21 26578.0 22 29709.0 23 33184.0 24 38555.0 25 44959.0 26 53306.0 27 63696.0 28 76422.0 29 90632.0 30 107392.0 31 127590.0 32 153303.0 33 187877.0 34 236321.0 35 306310.0 36 402878.0 37 460019.0 38 222509.0 39 7883.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.82746389290137 19.616245338334746 15.094123410688415 23.462167358075465 2 15.817961508848647 20.637419412745583 39.423207726839884 24.121411351565886 3 19.259050556681032 23.969296424282373 29.84970961333525 26.92194340570134 4 13.331714330870136 17.126522939898216 36.49911392906316 33.042648800168486 5 13.992068060973276 37.18916819118554 34.28530158126907 14.533462166572123 6 32.55616488184953 36.366780709467 17.100918241369605 13.97613616731386 7 29.093929827106656 32.06140014276658 20.417162968016992 18.427507062109772 8 26.28387420981641 35.801576179497616 19.46394898530247 18.450600625383508 9 26.08090316507973 16.834611889715795 19.8357939016873 37.24869104351718 10-11 24.963633770428956 26.734146128639207 27.43694037970864 20.865279721223196 12-13 25.723478584487225 23.73016064919896 27.860299010831547 22.686061755482267 14-15 22.430455609020015 25.375147751496442 25.848267930189454 26.346128709294092 16-17 22.441108785401223 28.60549570736566 26.53874444606812 22.414651061165003 18-19 22.317983998508556 27.257571026724403 28.634704334055577 21.789740640711464 20-21 22.83967425109747 26.496324478931765 28.74304083005065 21.920960439920115 22-23 22.59358237235725 26.379332277126966 28.566492466206828 22.46059288430896 24-25 22.531200268029146 26.544207812306702 28.639482221515856 22.285109698148293 26-27 22.27299512029988 26.904443630255788 28.462937390586095 22.359623858858235 28-29 22.200061185720386 27.03490704921876 28.56912321843898 22.195908546621872 30-31 22.359082312183048 26.71595125112009 28.4833206766418 22.441645760055064 32-33 22.207706419446346 26.702685559793878 28.41789041542335 22.67171760533643 34-35 22.69370925190417 26.826114880168504 28.286417987735156 22.193757880192162 36-37 22.71370223251567 26.536152522960904 28.306216640860022 22.443928603663405 38-39 22.372340537377035 26.816547455922397 28.273307423541 22.53780458315957 40-41 22.683810529731634 26.57737989898293 28.290387818516898 22.448421752768542 42-43 22.57904049280042 26.619016396659763 28.125796152116877 22.676146958422944 44-45 22.55146631826005 26.813057257538542 28.127362337389705 22.508114086811702 46-47 22.626680810160046 26.96974252604334 27.74887870062086 22.654697963175753 48-49 22.576849175903217 26.93760778018292 27.772007307237956 22.713535736675908 50-51 22.719775805645895 27.03019751748277 27.64652523456811 22.603501442303227 52-53 22.921430371154564 27.00618760313694 27.512896826439782 22.559485199268718 54-55 22.533374918741995 26.97516965620198 27.708094961916057 22.783360463139967 56-57 22.572293541266518 27.20066063710901 27.574064982273327 22.65298083935115 58-59 22.603936276485744 26.860530328256715 27.643737184317985 22.891796210939557 60-61 22.52724532337822 26.997304115595373 27.73636822704162 22.73908233398479 62-63 22.43823591980842 27.072211044198735 27.78215669692502 22.707396339067827 64-65 22.298955842126652 26.92137368367465 27.66721325351308 23.112457220685624 66-67 22.274309823704506 27.035579591434068 27.590580354553545 23.09953023030788 68-69 22.418362516917902 26.853389968478332 27.66578231207874 23.06246520252503 70-71 22.387523851409007 26.925835916323386 27.480099733495 23.206540498772604 72-73 22.457546266452646 26.866545895518527 27.454556190560965 23.221351647467863 74-75 22.364607354580865 27.073253829466626 27.25515371320072 23.30698510275179 76-77 22.343219257426714 26.799581085894925 27.60141872617575 23.255780930502613 78-79 22.389501903016694 26.952588023833496 27.481594239858097 23.176315833291707 80-81 22.33693087025995 27.14189393940722 27.363428229112024 23.157746961220806 82-83 22.39553325423442 27.04185540884259 27.361780079658647 23.200831257264344 84-85 22.373206184352938 26.917276288266667 27.384341300239456 23.325176227140943 86-87 22.4004033213379 26.913813720776027 27.334693625784528 23.351089332101544 88-89 22.317861574007093 26.904692384246776 27.212408973532725 23.565037068213403 90-91 22.220140500446302 26.934352329326906 27.425804193561333 23.41970297666546 92-93 22.230266665404795 26.699419292853133 27.41756548860265 23.652748553139418 94-95 22.07882457708201 26.59802368070846 27.450879013582487 23.872272728627046 96-97 22.21313271364192 26.446006040326104 27.417471371553674 23.923389874478307 98-99 22.01485302475843 26.535309856424032 27.223123903650638 24.2267132151669 100-101 22.007185254334203 26.413739358675482 27.607379702366234 23.971695684624077 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 351.0 1 256.0 2 201.0 3 255.0 4 234.5 5 241.5 6 443.0 7 1029.0 8 1640.5 9 1842.5 10 1627.0 11 1427.5 12 1580.5 13 1779.5 14 1944.0 15 2153.0 16 2349.0 17 2550.5 18 2818.0 19 3135.0 20 3587.5 21 4184.5 22 4895.5 23 5936.0 24 7328.5 25 9152.0 26 11727.0 27 15269.5 28 19008.0 29 23363.0 30 29018.5 31 34940.0 32 41841.5 33 47241.5 34 51642.0 35 57489.5 36 63840.0 37 69922.5 38 75108.0 39 79555.5 40 84791.5 41 91764.0 42 97955.0 43 103005.0 44 107891.5 45 112615.0 46 114892.5 47 116353.0 48 118660.5 49 118170.5 50 115377.0 51 111783.5 52 106673.5 53 102698.0 54 100219.0 55 92194.0 56 80985.0 57 72372.5 58 64336.0 59 55008.5 60 44914.5 61 36833.5 62 30295.5 63 22973.0 64 16747.5 65 12620.0 66 9326.5 67 7163.5 68 5453.0 69 3937.5 70 2983.0 71 2277.5 72 1833.5 73 1547.0 74 1272.0 75 1057.5 76 790.5 77 456.5 78 250.5 79 162.0 80 99.5 81 62.0 82 32.0 83 17.5 84 11.5 85 5.5 86 1.0 87 2.0 88 2.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.256501503885081E-4 2 0.049796590913471335 3 0.0 4 0.0 5 0.04706321013145108 6 7.008668671846773E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006588148551535967 26-27 0.0018397755263597781 28-29 7.008668671846773E-5 30-31 0.001611993794524758 32-33 4.906068070292741E-4 34-35 0.011616868323586028 36-37 0.023181171632133204 38-39 0.00417015785974883 40-41 0.0011213869874954836 42-43 0.0014017337343693547 44-45 0.00834031571949766 46-47 0.0 48-49 0.0 50-51 4.730851353496572E-4 52-53 0.0 54-55 0.0015419071078062903 56-57 0.0 58-59 0.02309356327373512 60-61 0.0 62-63 0.01023265626089629 64-65 0.015716939496616393 66-67 0.006640713566574819 68-69 0.01725884660442268 70-71 1.4017337343693546E-4 72-73 8.760835839808467E-5 74-75 0.023338866677249756 76-77 0.05161884476815149 78-79 0.031591574038349335 80-81 0.02724619946180433 82-83 0.037233552319185986 84-85 0.01657550140891762 86-87 0.010548046351129395 88-89 0.08573353952836565 90-91 0.025774379040716514 92-93 0.02481068709833758 94-95 0.027351329491882036 96-97 0.03903828450218653 98-99 0.05361631533962782 100-101 0.0141750323888101 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2853609.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.34118342235353 #Duplication Level Percentage of deduplicated Percentage of total 1 72.01549029461994 36.973604964668034 2 14.76462113700832 15.160662439134043 3 5.409419241130798 8.331779564019143 4 2.579108402152426 5.296579101641634 5 1.458303947846959 3.74355252359765 6 0.8895774851179259 2.7403176499101245 7 0.5812005363616786 2.0887666339760633 8 0.4085433788802403 1.6780080440862792 9 0.28403608207742514 1.3124473729653374 >10 1.4720387241046544 13.377686845865137 >50 0.09145904886983446 3.1977652947048822 >100 0.04278572627986614 4.071878259339556 >500 0.002322876973999366 0.845057034756613 >1k 9.564787539506446E-4 0.7487376499757292 >5k 1.3663982199294922E-4 0.4331566213599869 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6773 0.23734856457209097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5576 0.19540168257108806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4190 0.1468316086751899 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.504334335923387E-5 0.0 0.0 0.0 0.0 7 3.504334335923387E-5 0.0 0.0 0.0 0.0 8 3.504334335923387E-5 0.0 0.0 0.0 0.0 9 3.504334335923387E-5 0.0 0.0 0.0 0.0 10-11 3.504334335923387E-5 0.0 0.0 0.0 0.0 12-13 3.504334335923387E-5 0.0 0.0 0.0 8.760835839808468E-5 14-15 3.504334335923387E-5 0.0 0.0 0.0 1.2265170175731853E-4 16-17 3.504334335923387E-5 0.0 0.0 1.7521671679616935E-5 1.7521671679616933E-4 18-19 3.504334335923387E-5 0.0 0.0 7.008668671846774E-5 1.7521671679616933E-4 20-21 3.504334335923387E-5 0.0 0.0 1.576950451165524E-4 2.102600601554032E-4 22-23 5.25650150388508E-5 0.0 0.0 3.153900902331048E-4 2.102600601554032E-4 24-25 1.051300300777016E-4 0.0 0.0 7.359102105439113E-4 2.102600601554032E-4 26-27 1.051300300777016E-4 0.0 0.0 0.0011213869874954836 2.102600601554032E-4 28-29 1.7521671679616933E-4 0.0 0.0 0.0033641609624864514 2.102600601554032E-4 30-31 1.7521671679616933E-4 0.0 0.0 0.010267699604255522 2.102600601554032E-4 32-33 1.7521671679616933E-4 0.0 0.0 0.0251435988602503 2.102600601554032E-4 34-35 1.7521671679616933E-4 0.0 0.0 0.04949872249491784 2.2778173183502014E-4 36-37 1.7521671679616933E-4 0.0 0.0 0.09198877631798891 2.4530340351463706E-4 38-39 1.7521671679616933E-4 0.0 0.0 0.1546813175876583 2.4530340351463706E-4 40-41 1.7521671679616933E-4 0.0 0.0 0.22289318543640702 2.4530340351463706E-4 42-43 2.2778173183502014E-4 0.0 0.0 0.29825389533043944 2.4530340351463706E-4 44-45 2.8034674687387097E-4 0.0 0.0 0.39146918866600156 2.803467468738709E-4 46-47 2.8034674687387097E-4 0.0 0.0 0.5030296722501226 3.3291176191272174E-4 48-49 2.8034674687387097E-4 0.0 0.0 0.6227026898219062 3.8547677695157257E-4 50-51 3.153900902331048E-4 0.0 0.0 0.7479826423311673 3.8547677695157257E-4 52-53 3.153900902331048E-4 0.0 0.0 0.8724390762714864 4.205201203108064E-4 54-55 3.153900902331048E-4 0.0 0.0 1.011911582841237 4.205201203108064E-4 56-57 3.153900902331048E-4 0.0 0.0 1.1783148987825594 4.205201203108064E-4 58-59 3.153900902331048E-4 0.0 0.0 1.3572812533181666 4.205201203108064E-4 60-61 3.153900902331048E-4 0.0 0.0 1.552665414217575 4.205201203108064E-4 62-63 3.153900902331048E-4 0.0 0.0 1.7465777546958956 4.205201203108064E-4 64-65 3.5043343359233867E-4 0.0 0.0 1.954630084219667 4.205201203108064E-4 66-67 3.5043343359233867E-4 0.0 0.0 2.196271458353264 4.205201203108064E-4 68-69 3.5043343359233867E-4 0.0 0.0 2.461567089254344 4.205201203108064E-4 70-71 3.5043343359233867E-4 0.0 0.0 2.741896314456535 4.205201203108064E-4 72-73 3.6795510527195565E-4 0.0 0.0 3.0328611943682544 4.3804179199042335E-4 74-75 3.8547677695157257E-4 0.0 0.0 3.3288372723803437 4.5556346367004027E-4 76-77 3.8547677695157257E-4 0.0 0.0 3.6524625483028683 4.906068070292741E-4 78-79 3.8547677695157257E-4 0.0 0.0 4.009081131998112 4.906068070292741E-4 80-81 4.205201203108064E-4 0.0 0.0 4.394487822262966 5.08128478708891E-4 82-83 4.205201203108064E-4 0.0 0.0 4.7872185712899 5.957368371069758E-4 84-85 4.5556346367004027E-4 0.0 0.0 5.185889167016224 5.957368371069758E-4 86-87 4.906068070292741E-4 0.0 0.0 5.601888696033689 6.833451955050604E-4 88-89 4.906068070292741E-4 0.0 0.0 6.062515922819139 7.709535539031451E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3985 0.0 52.9657 1 GTATCAA 7660 0.0 41.828392 1 ATCAACG 9505 0.0 33.138077 3 TCAACGC 9640 0.0 32.674007 4 TATCAAC 9810 0.0 32.224415 2 CAACGCA 9785 0.0 32.141273 5 AACGCAG 10120 0.0 31.062603 6 ACGCAGA 11790 0.0 26.461346 7 CGCAGAG 11900 0.0 26.136938 8 GTACATG 9400 0.0 23.617237 1 TACATGG 9190 0.0 23.535767 2 GTGGTAT 2285 0.0 22.884768 1 GCAGAGT 13665 0.0 22.51779 9 ACATGGG 9435 0.0 22.205605 3 TGGTATC 2215 0.0 21.246819 2 TAGACCG 140 4.8487188E-4 20.36053 4 GAGTACT 7535 0.0 20.3554 12-13 AGAGTAC 12125 0.0 20.110325 10-11 CAGAGTA 12820 0.0 19.520142 10-11 AGTACTT 8275 0.0 18.707249 12-13 >>END_MODULE