FastQCFastQC Report
Thu 26 May 2016
SRR938410_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938410_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882674
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68860.23887543301809364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53020.18392645162096025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37040.12849181003471083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.051.4101561
GTATCAA75850.042.5656551
ATCAACG96350.032.703493
TCAACGC97850.032.2507324
TATCAAC99150.032.019612
CAACGCA99500.031.6165495
AACGCAG103500.030.4363386
ACGCAGA118150.026.5389797
CGCAGAG118650.026.2669758
GTACATG96200.026.0867981
TACATGG95450.025.5929012
ACATGGG100150.023.3003733
GCAGAGT133450.022.8894969
AGAGTAC121550.020.28341710-11
GAGTACT72200.020.26233712-13
CATGGGG59300.019.8760154
CAGAGTA126200.019.59251210-11
TAGGTCG1951.0297188E-519.4955235
AGTACTT77950.019.22468612-13
GTATAGA10700.018.6917841
Produced by FastQC (version 0.11.5)