##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938410_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2882674 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0774725133678 31.0 31.0 34.0 28.0 34.0 2 31.167941640296476 31.0 31.0 34.0 27.0 34.0 3 31.239278184074927 31.0 31.0 34.0 27.0 34.0 4 34.865362854072295 37.0 35.0 37.0 32.0 37.0 5 34.88606724173459 37.0 35.0 37.0 32.0 37.0 6 34.91477912521499 37.0 35.0 37.0 32.0 37.0 7 34.83879689482751 37.0 35.0 37.0 32.0 37.0 8 34.802431700566906 37.0 35.0 37.0 32.0 37.0 9 36.31029141692748 39.0 35.0 39.0 32.0 39.0 10-11 36.16609266257648 39.0 35.0 39.0 31.5 39.0 12-13 36.01929389171305 38.5 35.0 39.0 30.0 39.0 14-15 37.01816698662422 39.0 36.0 41.0 31.0 41.0 16-17 36.87216851437242 39.0 36.0 41.0 31.0 41.0 18-19 36.86078012983778 39.0 36.0 41.0 30.5 41.0 20-21 36.953351471585066 39.0 36.0 41.0 30.5 41.0 22-23 36.87323939508942 39.0 36.0 41.0 30.0 41.0 24-25 36.76517115705765 39.0 36.0 41.0 30.0 41.0 26-27 36.58297313535974 39.0 36.0 41.0 30.0 41.0 28-29 36.408325221651836 39.0 36.0 41.0 29.5 41.0 30-31 36.20806983377239 39.0 35.0 40.0 29.0 41.0 32-33 35.95045884480868 38.5 35.0 40.0 28.0 41.0 34-35 35.709824801555776 38.0 35.0 40.0 27.0 41.0 36-37 35.432516822922054 38.0 34.0 40.0 26.5 41.0 38-39 35.196316683745714 38.0 34.0 40.0 25.5 41.0 40-41 35.15070885573603 38.0 34.0 40.0 25.0 41.0 42-43 34.99878602297728 38.0 34.0 40.0 25.0 41.0 44-45 34.73047594004733 38.0 33.5 40.0 24.0 41.0 46-47 34.401960818323545 38.0 33.0 40.0 24.0 41.0 48-49 34.0720192432443 38.0 33.0 40.0 23.0 41.0 50-51 33.35066469534883 37.5 32.0 39.5 21.5 40.5 52-53 33.42159276421822 37.5 32.5 39.5 22.0 40.5 54-55 33.56796346031497 38.0 33.0 40.0 20.5 41.0 56-57 33.28902348999574 37.5 32.0 40.0 19.5 41.0 58-59 32.970691621737316 37.0 32.0 40.0 18.0 41.0 60-61 32.533038942315365 36.5 31.0 39.5 14.0 41.0 62-63 32.015263605943645 36.0 31.0 39.0 10.5 40.5 64-65 31.531343467905145 35.5 30.0 39.0 8.0 40.0 66-67 30.996302218010083 35.0 29.0 38.5 7.0 40.0 68-69 30.435238427931843 35.0 29.0 38.0 2.0 40.0 70-71 29.819552609833785 34.0 28.0 37.0 2.0 39.5 72-73 29.26337369400772 34.0 28.0 37.0 2.0 39.0 74-75 28.64144211242756 34.0 26.0 36.0 2.0 39.0 76-77 27.899594612502142 33.5 26.0 35.5 2.0 38.5 78-79 27.121958119440492 32.5 25.0 35.0 2.0 37.0 80-81 26.457191309180295 32.0 23.5 35.0 2.0 37.0 82-83 25.786113518212602 32.0 21.0 35.0 2.0 36.0 84-85 25.11821992358484 31.0 19.0 34.5 2.0 36.0 86-87 24.44250338401082 31.0 15.5 34.0 2.0 35.0 88-89 23.65392999694034 30.5 7.5 34.0 2.0 35.0 90-91 22.81492357443124 30.0 2.0 34.0 2.0 35.0 92-93 21.959938411350016 29.0 2.0 34.0 2.0 35.0 94-95 20.997486014721055 29.0 2.0 34.0 2.0 35.0 96-97 19.762033965686026 27.0 2.0 33.5 2.0 35.0 98-99 18.283336582631264 25.0 2.0 33.0 2.0 34.5 100-101 14.810124037612301 8.5 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6455848357492755 1101 2 0.6533524619033066 1101 3 0.6555290448868405 1101 4 0.6018401878377944 1101 5 0.5739292860759662 1101 6 0.45354875796497396 1101 7 0.49588487386310476 1101 8 0.6017876066125041 1101 9 0.6236471532463028 1101 10-11 0.6955860769914395 1101 12-13 0.5772016231878752 1101 14-15 0.8442019782404557 1101 16-17 0.9274905729691412 1101 18-19 0.7858958438911827 1101 20-21 0.8978812308648898 1101 22-23 0.9952781587906188 1101 24-25 0.8690995832247026 1101 26-27 1.0230755166934955 1101 28-29 0.990090267472759 1101 30-31 0.9707312710875158 1101 32-33 0.9521651692566167 1101 34-35 0.9030071323571178 1101 36-37 0.9591722277638652 1101 38-39 0.9975847620082945 1101 40-41 0.9914492045549963 1101 42-43 0.9764426030486035 1101 44-45 0.8525923400631825 1101 46-47 1.070428445575395 1101 48-49 1.1280191966640345 1101 50-51 0.9813794597669627 1101 52-53 1.0816834063612077 1101 54-55 1.1860353801354648 1101 56-57 1.0745538871579328 1101 58-59 1.0425157309356408 1101 60-61 1.0593633909754487 1101 62-63 1.0906274662597397 1101 64-65 0.9611228059724688 1101 66-67 0.8506664236032684 1101 68-69 0.8418669816978834 1101 70-71 1.126530256740768 1101 72-73 1.132015770939205 1101 74-75 0.920126645794749 1101 76-77 1.2618314341888777 1101 78-79 1.1567914208854404 1101 80-81 1.1855276784532656 1101 82-83 1.0388210929460264 1101 84-85 1.2630780803879382 1101 86-87 1.3045756509892534 1101 88-89 1.4985712809870897 1101 90-91 1.6078795560302517 1101 92-93 1.4194438268800873 1101 94-95 1.4575357558337103 1101 96-97 1.5086221922913055 1101 98-99 1.6026945354979105 1101 100-101 1.4259010683567102 1102 1 0.5211027990158072 1102 2 0.5758644758014775 1102 3 0.48894846538250647 1102 4 0.6166695636602313 1102 5 0.5922830112671562 1102 6 0.5951372575295366 1102 7 0.5154722328398833 1102 8 0.5431863468614182 1102 9 0.6346512359955341 1102 10-11 0.5809311717019838 1102 12-13 0.6908570583810913 1102 14-15 0.7592699408441419 1102 16-17 0.707047327123405 1102 18-19 0.7700329884842887 1102 20-21 0.6235460172425675 1102 22-23 0.8389481257611422 1102 24-25 0.6464426851898608 1102 26-27 0.5581073957877152 1102 28-29 0.5408980888373307 1102 30-31 0.5587747120448796 1102 32-33 0.5272252867572362 1102 34-35 0.6133665959215548 1102 36-37 0.6390705263850123 1102 38-39 0.8388525688249899 1102 40-41 0.8438426981744058 1102 42-43 0.972065166863473 1102 44-45 0.9553604026634304 1102 46-47 0.8597843306478623 1102 48-49 0.6020576294919451 1102 50-51 0.7999378489784874 1102 52-53 0.8864665899887783 1102 54-55 0.7699054418369755 1102 56-57 0.6901088358977745 1102 58-59 0.8147522060312227 1102 60-61 0.815312232775824 1102 62-63 0.6668140473918243 1102 64-65 0.6713218240460641 1102 66-67 0.6655243450945001 1102 68-69 0.6668704476921725 1102 70-71 0.717373378591251 1102 72-73 0.7981926968457245 1102 74-75 0.7504848406627964 1102 76-77 0.9226981718810663 1102 78-79 0.8458266255996385 1102 80-81 0.6813052363291767 1102 82-83 0.8855499934657338 1102 84-85 0.8575416950055548 1102 86-87 0.7212641749985274 1102 88-89 0.860835579437019 1102 90-91 0.8621458264110125 1102 92-93 0.9155900996446071 1102 94-95 0.7954765178717373 1102 96-97 0.7138578074238282 1102 98-99 0.768709219419673 1102 100-101 0.7876874994257985 1103 1 0.6326561640418085 1103 2 0.6467622500498571 1103 3 0.6015996127610741 1103 4 0.639092400602209 1103 5 0.6167944759416315 1103 6 0.649265348361709 1103 7 0.6103405529055976 1103 8 0.5455230156212636 1103 9 0.5519905146409201 1103 10-11 0.5995507928142132 1103 12-13 0.7113297941285666 1103 14-15 0.7950481506856519 1103 16-17 0.8051393413532182 1103 18-19 0.7558328494662234 1103 20-21 0.7315276034631424 1103 22-23 0.8105987986368675 1103 24-25 0.8398913090476938 1103 26-27 0.7992841964109232 1103 28-29 0.7297315790994787 1103 30-31 0.8799177699771406 1103 32-33 1.042111644644585 1103 34-35 0.948119241832373 1103 36-37 0.906584632594523 1103 38-39 0.6922265142310238 1103 40-41 0.8168323610136454 1103 42-43 0.7476781303099713 1103 44-45 0.8269117152668173 1103 46-47 0.7898545038401963 1103 48-49 0.8271645724588197 1103 50-51 0.8475044214399148 1103 52-53 0.7994634123302617 1103 54-55 0.7347355858830014 1103 56-57 0.8267401797958058 1103 58-59 0.8016319865754511 1103 60-61 0.8553707987510251 1103 62-63 1.029776474870694 1103 64-65 0.8961178465661312 1103 66-67 1.0588209673967093 1103 68-69 0.9991233819252159 1103 70-71 0.9758285325577098 1103 72-73 0.94335652021174 1103 74-75 0.8899880177218868 1103 76-77 0.9680083981966234 1103 78-79 0.8676020263075799 1103 80-81 0.9989293245775954 1103 82-83 1.11186770992499 1103 84-85 1.2214289115056864 1103 86-87 1.010507826095317 1103 88-89 1.0637257669147928 1103 90-91 1.1412815359030581 1103 92-93 1.3307991506365155 1103 94-95 1.2875656395283706 1103 96-97 1.259096043098051 1103 98-99 1.3666324962178749 1103 100-101 1.0892095410300353 1104 1 0.4129925596341053 1104 2 0.5371138698420133 1104 3 0.4483310548562791 1104 4 0.46072876402742935 1104 5 0.3968633158018591 1104 6 0.42726817952869567 1104 7 0.41802616098522805 1104 8 0.36073780922361465 1104 9 0.32989266402852024 1104 10-11 0.4720312225756018 1104 12-13 0.46355176087222105 1104 14-15 0.5019250529848378 1104 16-17 0.544770453532692 1104 18-19 0.4607325218730267 1104 20-21 0.5553121351651171 1104 22-23 0.5551722597559348 1104 24-25 0.6746104974619698 1104 26-27 0.576157347820299 1104 28-29 0.6450201711687242 1104 30-31 0.5923258412417312 1104 32-33 0.628656459649072 1104 34-35 0.5608425388064688 1104 36-37 0.648548960819447 1104 38-39 0.6276988999014605 1104 40-41 0.6055932783674862 1104 42-43 0.5147113506520995 1104 44-45 0.4478463255284595 1104 46-47 0.6260777642344024 1104 48-49 0.722768889582035 1104 50-51 0.60603908930036 1104 52-53 0.7181284439341198 1104 54-55 0.7523461297155691 1104 56-57 0.7168180310817291 1104 58-59 0.6681508821183968 1104 60-61 0.7439975898589779 1104 62-63 0.8151122375729116 1104 64-65 0.903473538918842 1104 66-67 0.8681743279268979 1104 68-69 0.8155080350956503 1104 70-71 0.9338242567755692 1104 72-73 0.6489098112982106 1104 74-75 0.8107570894869802 1104 76-77 0.8331293034268512 1104 78-79 0.8861781629839669 1104 80-81 0.880040226660789 1104 82-83 0.899129897123423 1104 84-85 0.6991739415766745 1104 86-87 0.9013359134085981 1104 88-89 0.9984069238610793 1104 90-91 0.9145562717440328 1104 92-93 1.2012334293566624 1104 94-95 1.1786172125214982 1104 96-97 0.9987879475630059 1104 98-99 0.8357486797397407 1104 100-101 0.6410328450878477 1105 1 0.4367825518957247 1105 2 0.4214452085818188 1105 3 0.4721924783214284 1105 4 0.48724334648086653 1105 5 0.3424667535702568 1105 6 0.4339974524327488 1105 7 0.4095303830795771 1105 8 0.4645029706382928 1105 9 0.5340668541205602 1105 10-11 0.5361013271996455 1105 12-13 0.5951149987466309 1105 14-15 0.740819626764484 1105 16-17 0.749242273639446 1105 18-19 0.7032598825750966 1105 20-21 0.6600932070300161 1105 22-23 0.6470183311814708 1105 24-25 0.618246742561702 1105 26-27 0.7409519689372885 1105 28-29 0.723213528633714 1105 30-31 0.6285553508324995 1105 32-33 0.7308441819857805 1105 34-35 0.7590491053749631 1105 36-37 0.8540843121787702 1105 38-39 0.7723972589679846 1105 40-41 0.8319550774133759 1105 42-43 0.8613944767310144 1105 44-45 0.7373805203825867 1105 46-47 0.8087843265510415 1105 48-49 0.6602779247789172 1105 50-51 0.5986680693931703 1105 52-53 0.6324127004401703 1105 54-55 0.6847432985454844 1105 56-57 0.8152170650597412 1105 58-59 0.9488998893097076 1105 60-61 0.8924735824550822 1105 62-63 0.8524696231908635 1105 64-65 0.8366556006161296 1105 66-67 0.9046113281564985 1105 68-69 0.8652850960417702 1105 70-71 0.923229195166158 1105 72-73 0.9399364080832378 1105 74-75 0.97949318237022 1105 76-77 1.072407678196896 1105 78-79 1.1987129219178279 1105 80-81 1.2878795177911329 1105 82-83 1.3385335599568045 1105 84-85 1.2528425858294376 1105 86-87 1.3297128093826593 1105 88-89 1.342771653853962 1105 90-91 1.2728475353109978 1105 92-93 1.3282857300366757 1105 94-95 1.3951470166242572 1105 96-97 1.4598273282658063 1105 98-99 1.5138124972840359 1105 100-101 1.3057483816580717 1106 1 0.3859510792635099 1106 2 0.45229969571160566 1106 3 0.16569024129664456 1106 4 0.4009541525293372 1106 5 0.45977141342033434 1106 6 0.1435587322976275 1106 7 0.2557665421685442 1106 8 0.2730173307142252 1106 9 0.36627064007792853 1106 10-11 0.3265950456251332 1106 12-13 0.3391548518688481 1106 14-15 0.5464734621701197 1106 16-17 0.24615456029098226 1106 18-19 0.020213133470491584 1106 20-21 0.37251735429156696 1106 22-23 0.4107772467892161 1106 24-25 0.40748771595335853 1106 26-27 0.3004616251533534 1106 28-29 0.34429036838509575 1106 30-31 0.39884729358296056 1106 32-33 0.5444390230792138 1106 34-35 0.47353022216377383 1106 36-37 0.3281735912493531 1106 38-39 0.4091092313595084 1106 40-41 0.5632793369584519 1106 42-43 0.07650232860154205 1106 44-45 0.3678808653769323 1106 46-47 0.20720220534618505 1106 48-49 0.6073639915254176 1106 50-51 0.21698590261670603 1106 52-53 0.3961149443815799 1106 54-55 0.2394826060913573 1106 56-57 -0.09262080488882418 1106 58-59 -0.5022852234451491 1106 60-61 0.38583072606922286 1106 62-63 0.686351303238034 1106 64-65 0.6769957867959384 1106 66-67 0.09356905477483224 1106 68-69 -0.03126250620568527 1106 70-71 0.02398645357389384 1106 72-73 0.471062372575318 1106 74-75 0.4538177381028454 1106 76-77 0.4099671597403969 1106 78-79 0.6383117467616586 1106 80-81 0.38125060620506446 1106 82-83 0.4364106036369364 1106 84-85 0.44231442932639453 1106 86-87 0.5606055052638617 1106 88-89 0.34001493427329166 1106 90-91 0.4696443032410862 1106 92-93 0.422319698453375 1106 94-95 0.33821329960440494 1106 96-97 0.08906293569849666 1106 98-99 0.030877864369735164 1106 100-101 -0.19088818841203725 1107 1 0.3838481951784267 1107 2 0.36844375938424534 1107 3 0.24934990411714608 1107 4 0.18956098911947805 1107 5 0.3327104334586153 1107 6 0.2908006299326118 1107 7 0.3308260730586454 1107 8 0.2651357869122464 1107 9 0.29834735436209314 1107 10-11 0.4010637554106893 1107 12-13 0.3486086383795026 1107 14-15 0.5614983342949458 1107 16-17 0.4360019752120934 1107 18-19 0.3516939440854827 1107 20-21 0.4350958907292224 1107 22-23 0.5006426669659163 1107 24-25 0.4431286597964217 1107 26-27 0.3784351958172181 1107 28-29 0.4038460765377394 1107 30-31 0.44841338314424206 1107 32-33 0.4927565860435337 1107 34-35 0.4992216672342238 1107 36-37 0.5483395856477173 1107 38-39 0.674162327939051 1107 40-41 0.5130280203579289 1107 42-43 0.3777604796294085 1107 44-45 0.4546702525494055 1107 46-47 0.5744785995369242 1107 48-49 0.5726095226534156 1107 50-51 0.5664320112105727 1107 52-53 0.4747182664323333 1107 54-55 0.4829720570662346 1107 56-57 0.49926596205936846 1107 58-59 0.4918484696328207 1107 60-61 0.4843575393011932 1107 62-63 0.510401296208947 1107 64-65 0.4902361921034277 1107 66-67 0.6260954138201775 1107 68-69 0.612919146817358 1107 70-71 0.710214595842448 1107 72-73 0.5133948912564428 1107 74-75 0.5938265514862096 1107 76-77 0.6644781521381766 1107 78-79 0.764853494082498 1107 80-81 0.6562011323045773 1107 82-83 0.6428387502310748 1107 84-85 0.5887373311463868 1107 86-87 0.5656215099355002 1107 88-89 0.7340504365280047 1107 90-91 0.8361796162513677 1107 92-93 0.8860523555532893 1107 94-95 0.8126420517807169 1107 96-97 0.8449969380559459 1107 98-99 0.5503730683752295 1107 100-101 0.22931662812900733 1108 1 0.30336593175542603 1108 2 0.39255371544468787 1108 3 0.344428673809567 1108 4 0.36839712672530567 1108 5 0.36767667501900547 1108 6 0.30285721550757927 1108 7 0.3961716951784595 1108 8 0.3670551953357801 1108 9 0.383503286202199 1108 10-11 0.25386463420181116 1108 12-13 0.3148619874310512 1108 14-15 0.5114806513027688 1108 16-17 0.4276030897764542 1108 18-19 0.338530831878856 1108 20-21 0.42301519198311155 1108 22-23 0.46526864488897246 1108 24-25 0.4483505009709958 1108 26-27 0.432947038063503 1108 28-29 0.40224764121462897 1108 30-31 0.5349823468137274 1108 32-33 0.39699085889746755 1108 34-35 0.6047832361978962 1108 36-37 0.5170706969969814 1108 38-39 0.6259375931839344 1108 40-41 0.48294871481725465 1108 42-43 0.4763924590528319 1108 44-45 0.4271356903374226 1108 46-47 0.6473164518571437 1108 48-49 0.679323701198058 1108 50-51 0.5947612363740049 1108 52-53 0.6029258540435265 1108 54-55 0.6175289105653263 1108 56-57 0.6469280561543229 1108 58-59 0.9277797738874582 1108 60-61 0.8510824348107846 1108 62-63 0.7138280457534805 1108 64-65 0.7128157655682266 1108 66-67 0.761764688281648 1108 68-69 0.6634957195319657 1108 70-71 0.6372990455262979 1108 72-73 0.455222669629606 1108 74-75 0.5980442130444565 1108 76-77 0.7121509631980842 1108 78-79 0.6850747994088309 1108 80-81 0.7321844138392457 1108 82-83 0.954128001115226 1108 84-85 0.834819744606893 1108 86-87 0.8842995723082332 1108 88-89 0.9917918361636247 1108 90-91 0.9672491693539307 1108 92-93 0.8095600181832303 1108 94-95 0.7817396833314625 1108 96-97 1.0265964450755547 1108 98-99 0.8709700033232508 1108 100-101 0.24808203376612958 1109 1 0.5020390063721045 1109 2 0.5377354505615983 1109 3 0.4416720765843394 1109 4 0.45369102981722165 1109 5 0.3793530496392705 1109 6 0.5084903426473559 1109 7 0.5181771181142878 1109 8 0.44917446777710524 1109 9 0.611434620410094 1109 10-11 0.47233637027076725 1109 12-13 0.41488257381535476 1109 14-15 0.3345435219967854 1109 16-17 0.5638380317327361 1109 18-19 0.48732037721032384 1109 20-21 0.5355926072976516 1109 22-23 0.5352702645984877 1109 24-25 0.4253017872799987 1109 26-27 0.40170073959430397 1109 28-29 0.4580946689240264 1109 30-31 0.3411499162726841 1109 32-33 0.36543471370719516 1109 34-35 0.4521421205518763 1109 36-37 0.44181726191456505 1109 38-39 0.560842580529183 1109 40-41 0.5002029486266437 1109 42-43 0.3480138500989298 1109 44-45 0.4093676005931499 1109 46-47 0.5414840138975876 1109 48-49 0.4221137064775675 1109 50-51 0.4124784474567704 1109 52-53 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-1.2026333340075581 2316 56-57 -1.2209649261421376 2316 58-59 -1.2857863072120708 2316 60-61 -1.2200315459838258 2316 62-63 -1.1880134187711633 2316 64-65 -1.3390689338355877 2316 66-67 -1.3486549175982958 2316 68-69 -1.1792140834080058 2316 70-71 -1.626926367480575 2316 72-73 -1.4058505793577822 2316 74-75 -1.3452521051642528 2316 76-77 -1.1100114634163987 2316 78-79 -1.1338683353474615 2316 80-81 -1.1904018283670972 2316 82-83 -0.9047772462337917 2316 84-85 -1.0816681041165417 2316 86-87 -1.072925772433038 2316 88-89 -1.098448458743757 2316 90-91 -1.167916716050886 2316 92-93 -1.1185654084767584 2316 94-95 -1.1656224201591847 2316 96-97 -1.5004725714447211 2316 98-99 -1.4460332506702862 2316 100-101 -1.1751464739478816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8988.0 3 3247.0 4 3296.0 5 3775.0 6 4435.0 7 5597.0 8 7096.0 9 9097.0 10 11335.0 11 13957.0 12 17155.0 13 19818.0 14 22653.0 15 26090.0 16 29091.0 17 33614.0 18 32830.0 19 31406.0 20 34113.0 21 36904.0 22 40683.0 23 45443.0 24 50804.0 25 57443.0 26 65021.0 27 73662.0 28 84208.0 29 96839.0 30 111932.0 31 131713.0 32 156075.0 33 187380.0 34 228395.0 35 284929.0 36 349926.0 37 367550.0 38 185923.0 39 10251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13306466187154 18.74866197163519 14.483653116320191 23.634620250173075 2 15.354828095812923 19.86264047108182 40.41700709218873 24.365524340916522 3 19.286582714124624 23.71354466198649 29.356757580353676 27.64311504353521 4 12.877547266081923 16.406771189470433 36.86511421262534 33.850567331822305 5 14.113007501597616 36.998763907773146 34.189005825705095 14.699222764924139 6 32.708044128831865 35.82643668465158 17.407047547042488 14.058471639474067 7 29.22931864423602 31.926680832442333 20.43574508960842 18.40825543371323 8 26.28181570532007 35.357809021614464 19.713426579008097 18.646948694057368 9 26.36153041269224 16.81739543874688 19.91216212698837 36.90891202157251 10-11 24.952011025230522 26.515353174907535 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22.29204224164202 36-37 22.800769602004976 26.463806510380376 28.188833968276388 22.546589919338256 38-39 22.429164380070237 26.808273422048938 28.148983779580373 22.61357841830045 40-41 22.68854435170668 26.552156622979656 28.250219516524073 22.509079508789593 42-43 22.55834594903315 26.527737248312018 28.09496976222341 22.81894704043142 44-45 22.490754745781526 26.831444965586144 28.08134089142984 22.596459397202487 46-47 22.636670930558925 26.948475597803593 27.644107588860866 22.770745882776616 48-49 22.570149762226833 26.936297885524024 27.707521139729156 22.78603121251998 50-51 22.730937585973972 27.03469280772741 27.576964161624307 22.657405444674307 52-53 22.846960791762637 26.96078482392496 27.55003765986361 22.642216724448797 54-55 22.483830722356103 26.975736195659188 27.745273075351157 22.795160006633548 56-57 22.568824845203185 27.147896333186754 27.596410292437834 22.686868529172227 58-59 22.60689864055762 26.770989981513164 27.73026881283021 22.891842565099 60-61 22.53504781317085 26.883830257822495 27.85365917123837 22.727462757768286 62-63 22.46308836589698 26.99062638765542 27.869012266873888 22.67727297957371 64-65 22.319623170732765 26.826812744961142 27.79684801849801 23.056716065808086 66-67 22.286917880013803 27.000528870813113 27.619701469858438 23.092851779314646 68-69 22.498034606196143 26.78077168334158 27.785186793941385 22.936006916520896 70-71 22.446281938066132 26.83550518833635 27.581477092727308 23.13673578087021 72-73 22.52430398211266 26.84865709612434 27.52047335947687 23.106565562286132 74-75 22.43309904909386 27.0166397731052 27.449909187605105 23.100351990195836 76-77 22.517287523319204 26.81388455879215 27.528127895926875 23.14070002196177 78-79 22.55132795983545 26.92092604374888 27.478454780537454 23.049291215878206 80-81 22.54829804414062 27.212837500878145 27.25587317226324 22.982991282717997 82-83 22.623257927735168 27.078505024678908 27.30372442886396 22.99451261872197 84-85 22.64104960500005 26.980810620662666 27.294267678554828 23.083872095782457 86-87 22.717022423690274 26.900604982379257 27.276663389302936 23.105709204627537 88-89 22.711003344336532 26.920876728015653 27.169459397325397 23.198660530322417 90-91 22.660294350877155 26.977241580592516 27.231776762806664 23.130687305723665 92-93 22.817215602392196 26.664516506240293 27.295025894744036 23.22324199662347 94-95 22.652075577768773 26.646655105073712 27.3228608088893 23.378408508268215 96-97 22.84463970904649 26.50441080525826 27.214373464373466 23.436576021321784 98-99 22.79138542379786 26.505838482247913 26.98396147951474 23.71881461443949 100-101 23.325348049319114 26.076623992808727 27.153022216386958 23.4450057414852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 373.0 1 257.0 2 204.5 3 284.5 4 256.0 5 257.5 6 472.5 7 1053.0 8 1710.0 9 1983.0 10 1811.5 11 1563.0 12 1600.0 13 1828.5 14 2074.0 15 2215.5 16 2329.5 17 2529.0 18 2824.5 19 3219.5 20 3617.0 21 4223.0 22 5083.5 23 6070.0 24 7376.0 25 9350.5 26 12019.0 27 15572.5 28 19277.5 29 23794.0 30 29139.5 31 34793.0 32 41848.0 33 47399.5 34 51924.0 35 57377.0 36 63653.5 37 70250.0 38 75472.0 39 79849.5 40 85111.0 41 92020.5 42 98172.0 43 103358.5 44 108072.0 45 112133.5 46 114484.5 47 116523.5 48 118904.5 49 118301.0 50 115441.0 51 111671.5 52 107582.5 53 104073.0 54 100822.5 55 92957.0 56 82229.5 57 73810.5 58 66108.0 59 57214.0 60 46947.5 61 38459.5 62 31677.0 63 24302.0 64 18212.0 65 13901.5 66 10320.0 67 7801.0 68 5946.5 69 4467.0 70 3503.0 71 2730.0 72 2182.0 73 1795.5 74 1378.0 75 1089.0 76 862.5 77 544.0 78 311.5 79 192.5 80 110.5 81 69.5 82 43.0 83 28.0 84 14.5 85 7.0 86 8.0 87 6.0 88 5.0 89 4.5 90 3.5 91 2.0 92 2.5 93 3.0 94 2.0 95 1.0 96 0.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18406521167499343 2 0.0512024599382379 3 0.050057689492464294 4 0.05703038220763083 5 0.06317051459859838 6 0.06684765603047726 7 0.03749990460246286 8 0.036042924035114624 9 0.04263402660168996 10-11 0.044507287331137686 12-13 0.04136784110863733 14-15 0.036892829366067754 16-17 0.03470735851504541 18-19 0.04357065696641382 20-21 0.05444597620126313 22-23 0.051237149951746185 24-25 0.07083700758393076 26-27 0.056457996984744026 28-29 0.04820177376977071 30-31 0.057307902315697164 32-33 0.05304103065417733 34-35 0.05328386074873537 36-37 0.05010972451272673 38-39 0.029261026394243675 40-41 0.026798035435154997 42-43 0.02369327922616293 44-45 0.003954661539945204 46-47 0.011482394471244406 48-49 0.010580454120028834 50-51 0.009054093525664018 52-53 0.010233553984945921 54-55 0.012349644808951689 56-57 0.00924488859995962 58-59 0.012228229761672668 60-61 0.01254043988324729 62-63 0.003954661539945204 64-65 0.006990037721920688 66-67 0.004076076587224223 68-69 0.010407004052487378 70-71 0.02528901984754433 72-73 0.02206284859127324 74-75 0.021976123557502513 76-77 0.013633175308758464 78-79 0.0045790817830944465 80-81 0.006296237451754863 82-83 0.005255537046506126 84-85 0.004925981918177358 86-87 0.009574443728288388 88-89 0.03248719765051476 90-91 0.039789445494010076 92-93 0.02492477470570727 94-95 0.03491549859609515 96-97 0.03864467504823647 98-99 0.0234157591180966 100-101 0.1258553690080807 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2882674.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.71176549577217 #Duplication Level Percentage of deduplicated Percentage of total 1 74.08513841837971 39.792435814498326 2 13.778369840818556 14.801211396081317 3 5.047110581504621 8.132676599550196 4 2.372816143012003 5.097925769521732 5 1.3009049486768758 3.493695076781095 6 0.8071691449133778 2.601268789620618 7 0.5097880580078179 1.916713163698271 8 0.36993890315399874 1.5896057291176602 9 0.27244355052548275 1.3170081686994257 >10 1.3359560736888534 12.685221986040007 >50 0.07892493848682289 2.9123502220987687 >100 0.03812908789125602 3.6856155232881944 >500 0.0023366900758045287 0.8685484890070422 >1k 8.438047495120568E-4 0.6808098138341866 >5k 1.2981611530954722E-4 0.42491345816320497 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6886 0.23887543301809364 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5302 0.18392645162096025 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3704 0.12849181003471083 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.469001350829126E-5 0.0 0.0 0.0 0.0 8 3.469001350829126E-5 0.0 0.0 0.0 0.0 9 3.469001350829126E-5 0.0 0.0 3.469001350829126E-5 0.0 10-11 3.469001350829126E-5 0.0 0.0 3.469001350829126E-5 0.0 12-13 3.469001350829126E-5 0.0 0.0 3.469001350829126E-5 6.938002701658252E-5 14-15 3.469001350829126E-5 0.0 0.0 3.469001350829126E-5 8.672503377072815E-5 16-17 3.469001350829126E-5 0.0 0.0 5.2035020262436896E-5 1.3876005403316505E-4 18-19 5.2035020262436896E-5 0.0 0.0 6.938002701658252E-5 1.3876005403316505E-4 20-21 6.938002701658252E-5 0.0 0.0 6.938002701658252E-5 2.428300945580388E-4 22-23 8.672503377072815E-5 0.0 0.0 1.2141504727901942E-4 2.428300945580388E-4 24-25 1.0407004052487378E-4 0.0 0.0 4.3362516885364074E-4 2.428300945580388E-4 26-27 1.0407004052487378E-4 0.0 0.0 9.713203782321554E-4 2.775201080663301E-4 28-29 2.4283009455803884E-4 0.0 0.0 0.0028966161279423202 2.775201080663301E-4 30-31 2.775201080663301E-4 0.0 0.0 0.009470373687763513 2.775201080663301E-4 32-33 2.775201080663301E-4 0.0 0.0 0.025809370050168697 3.1221012157462135E-4 34-35 2.775201080663301E-4 0.0 0.0 0.051358564999025205 3.1221012157462135E-4 36-37 2.775201080663301E-4 0.0 0.0 0.09477311690465173 3.1221012157462135E-4 38-39 2.775201080663301E-4 0.0 0.0 0.159955652286731 3.469001350829126E-4 40-41 2.775201080663301E-4 0.0 0.0 0.2302896546747915 3.469001350829126E-4 42-43 2.948651148204757E-4 0.0 0.0 0.3065383043660157 3.469001350829126E-4 44-45 3.469001350829126E-4 0.0 0.0 0.40223070662863714 3.989351553453495E-4 46-47 3.989351553453495E-4 0.0 0.0 0.5128051246863156 4.5097017560778637E-4 48-49 4.5097017560778637E-4 0.0 0.0 0.631011345715818 4.5097017560778637E-4 50-51 4.5097017560778637E-4 0.0 0.0 0.7596766058180703 4.5097017560778637E-4 52-53 4.5097017560778637E-4 0.0 0.0 0.8835026090359159 4.5097017560778637E-4 54-55 4.856601891160776E-4 0.0 0.0 1.0256449393861393 4.5097017560778637E-4 56-57 5.203502026243689E-4 0.0 0.0 1.191758069070592 4.856601891160777E-4 58-59 5.203502026243689E-4 0.0 0.0 1.3714349940367865 5.203502026243689E-4 60-61 5.203502026243689E-4 0.0 0.0 1.5612414029474024 5.203502026243689E-4 62-63 5.203502026243689E-4 0.0 0.0 1.7552453034925213 5.550402161326602E-4 64-65 5.203502026243689E-4 0.0 0.0 1.9646862600488295 5.550402161326602E-4 66-67 5.203502026243689E-4 0.0 0.0 2.1994856164796994 5.550402161326602E-4 68-69 5.203502026243689E-4 0.0 0.0 2.4613778734605436 5.550402161326602E-4 70-71 5.203502026243689E-4 0.0 0.0 2.747466414863422 5.723852228868058E-4 72-73 5.550402161326602E-4 0.0 0.0 3.03497724682014 6.070752363950971E-4 74-75 6.417652499033883E-4 0.0 0.0 3.3340398532751188 6.244202431492427E-4 76-77 6.59110256657534E-4 0.0 0.0 3.651332061828705 6.244202431492427E-4 78-79 6.764552634116796E-4 0.0 0.0 4.0037132190459275 6.244202431492427E-4 80-81 6.938002701658252E-4 0.0 0.0 4.3762145840979585 6.244202431492427E-4 82-83 6.938002701658252E-4 0.0 0.0 4.7637540700058345 6.59110256657534E-4 84-85 6.938002701658252E-4 0.0 0.0 5.158647838777469 6.59110256657534E-4 86-87 6.938002701658252E-4 0.0 0.0 5.568128758229339 7.111452769199708E-4 88-89 6.938002701658252E-4 0.0 0.0 6.006350353872827 7.631802971824077E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4020 0.0 51.410156 1 GTATCAA 7585 0.0 42.565655 1 ATCAACG 9635 0.0 32.70349 3 TCAACGC 9785 0.0 32.250732 4 TATCAAC 9915 0.0 32.01961 2 CAACGCA 9950 0.0 31.616549 5 AACGCAG 10350 0.0 30.436338 6 ACGCAGA 11815 0.0 26.538979 7 CGCAGAG 11865 0.0 26.266975 8 GTACATG 9620 0.0 26.086798 1 TACATGG 9545 0.0 25.592901 2 ACATGGG 10015 0.0 23.300373 3 GCAGAGT 13345 0.0 22.889496 9 AGAGTAC 12155 0.0 20.283417 10-11 GAGTACT 7220 0.0 20.262337 12-13 CATGGGG 5930 0.0 19.876015 4 CAGAGTA 12620 0.0 19.592512 10-11 TAGGTCG 195 1.0297188E-5 19.495523 5 AGTACTT 7795 0.0 19.224686 12-13 GTATAGA 1070 0.0 18.691784 1 >>END_MODULE