FastQCFastQC Report
Thu 26 May 2016
SRR938410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882674
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69090.23967330332878434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56520.1960679563488622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42200.14639185700498913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.053.7015461
GTATCAA75350.042.7307051
ATCAACG95500.033.0501253
TCAACGC98300.032.108164
TATCAAC99550.031.7692892
CAACGCA100300.031.4679165
AACGCAG103550.030.5471576
ACGCAGA118350.026.4462347
CGCAGAG119850.026.1152428
GTACATG91450.023.9746271
TACATGG91750.023.11832
GCAGAGT135050.022.7539279
GTATAGG8000.021.9961341
ACATGGG92650.021.5995673
GAGTACT75750.021.47457712-13
TCGCGTA1553.912312E-521.4492939
AGAGTAC121400.021.14577910-11
AGTACTT82300.020.48684912-13
TGGTATC21200.019.9655482
CAGAGTA129850.019.73313710-11
Produced by FastQC (version 0.11.5)