##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938410_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2882674 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80670238812991 31.0 31.0 34.0 30.0 34.0 2 32.03881847201591 31.0 31.0 34.0 30.0 34.0 3 32.11004470155141 33.0 31.0 34.0 30.0 34.0 4 35.767909586723995 37.0 35.0 37.0 35.0 37.0 5 35.63779393715696 37.0 35.0 37.0 33.0 37.0 6 35.66222923577207 37.0 35.0 37.0 33.0 37.0 7 35.60520371016632 37.0 35.0 37.0 33.0 37.0 8 35.59722431325915 37.0 35.0 37.0 33.0 37.0 9 37.185613426977866 39.0 37.0 39.0 33.0 39.0 10-11 37.095839487919896 39.0 37.0 39.0 33.0 39.0 12-13 36.989073686445295 39.0 37.0 39.0 33.0 39.0 14-15 38.06089450281232 40.0 37.0 41.0 33.0 41.0 16-17 37.98228485080172 40.0 37.0 41.0 33.0 41.0 18-19 38.08300973332399 40.0 37.5 41.0 33.0 41.0 20-21 38.14133613443629 40.0 37.0 41.0 33.0 41.0 22-23 38.04546941485579 40.0 37.0 41.0 33.0 41.0 24-25 37.94874116878981 40.0 37.0 41.0 33.0 41.0 26-27 37.76637091117483 40.0 37.0 41.0 32.0 41.0 28-29 37.65279251139741 40.0 37.0 41.0 32.0 41.0 30-31 37.43997881827775 39.0 36.5 41.0 32.0 41.0 32-33 37.249792380269156 39.0 36.0 41.0 31.0 41.0 34-35 37.1069496585462 39.0 36.0 41.0 31.0 41.0 36-37 37.115338744512904 39.0 36.0 41.0 31.0 41.0 38-39 37.013055586583846 39.0 36.0 41.0 31.0 41.0 40-41 36.85363520120555 39.0 35.5 41.0 30.0 41.0 42-43 36.665381343849496 39.0 35.0 41.0 30.0 41.0 44-45 36.42625440823347 39.0 35.0 40.0 30.0 41.0 46-47 36.09404029036929 39.0 35.0 40.0 29.5 41.0 48-49 35.772391363019196 38.0 35.0 40.0 28.5 41.0 50-51 35.43776663611632 38.0 34.0 40.0 27.0 41.0 52-53 34.9667881279673 38.0 34.0 40.0 26.0 41.0 54-55 34.67581852821374 38.0 33.0 40.0 26.0 41.0 56-57 34.35371949793837 38.0 33.0 40.0 25.0 41.0 58-59 33.92708506060693 37.0 33.0 40.0 24.0 41.0 60-61 33.56836534412147 37.0 32.0 39.0 23.0 41.0 62-63 33.11974975318056 36.0 31.5 39.0 22.5 40.0 64-65 32.62875545413738 35.5 31.0 39.0 21.5 40.0 66-67 32.13827890354581 35.0 31.0 38.5 20.0 40.0 68-69 31.597082084203763 35.0 30.0 38.0 18.5 40.0 70-71 31.02518200115587 34.0 29.5 37.0 16.5 39.0 72-73 30.576242058588658 34.0 29.0 37.0 13.0 39.0 74-75 30.043403451101305 34.0 29.0 36.0 9.5 39.0 76-77 28.755965294722884 32.5 27.0 35.0 8.0 37.0 78-79 29.4584609636747 34.0 29.0 35.0 7.5 37.0 80-81 29.173011759220778 34.0 29.0 35.0 6.0 37.0 82-83 28.695182146853927 33.0 28.0 35.0 2.0 36.5 84-85 28.16509966093981 33.0 26.5 35.0 2.0 36.0 86-87 27.583325412446914 32.5 26.0 35.0 2.0 35.5 88-89 26.944221233479748 32.0 25.0 34.5 2.0 35.0 90-91 26.399297839436578 32.0 25.0 34.0 2.0 35.0 92-93 25.65242601140469 31.5 23.0 34.0 2.0 35.0 94-95 24.872318895580978 31.0 19.0 34.0 2.0 35.0 96-97 23.866731895455402 31.0 8.5 34.0 2.0 35.0 98-99 22.607102814955837 30.5 2.0 34.0 2.0 35.0 100-101 18.88594669393764 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3406065902149855 1101 2 0.36202081785059903 1101 3 0.3758735039631347 1101 4 0.2780291990185617 1101 5 0.20324462379857522 1101 6 0.22418545033228554 1101 7 0.3521099549057851 1101 8 0.37828160000553623 1101 9 0.24417662752502167 1101 10-11 0.35735861386191203 1101 12-13 0.4558889610674015 1101 14-15 0.5456970527148641 1101 16-17 0.5202383739625844 1101 18-19 0.604462465610041 1101 20-21 0.5852201528377847 1101 22-23 0.5537583760954519 1101 24-25 0.5833367224064077 1101 26-27 0.6458598307914514 1101 28-29 0.61945137231708 1101 30-31 0.512482055414992 1101 32-33 0.4768258847673721 1101 34-35 0.4725146834385825 1101 36-37 0.6439428052352341 1101 38-39 0.6652242620556592 1101 40-41 0.6983662092912297 1101 42-43 0.5834601420306598 1101 44-45 0.545886349487283 1101 46-47 0.7157958917988338 1101 48-49 0.7721099210750992 1101 50-51 0.6486527374234967 1101 52-53 0.7402327961449444 1101 54-55 0.5526123923469441 1101 56-57 0.6582991567841319 1101 58-59 0.6270328408495089 1101 60-61 0.5927769562403213 1101 62-63 0.5381771448988601 1101 64-65 0.6288489077609043 1101 66-67 0.578544704249687 1101 68-69 0.7743638899606502 1101 70-71 0.5726674146001081 1101 72-73 0.5484553688991163 1101 74-75 0.558550267929359 1101 76-77 0.5702565092809841 1101 78-79 0.5686868968623386 1101 80-81 0.7082266741421357 1101 82-83 0.6782703379229638 1101 84-85 0.699697625517782 1101 86-87 0.7645537725711442 1101 88-89 0.7839866168075353 1101 90-91 0.8533322783506314 1101 92-93 0.6196686003273761 1101 94-95 0.6026300420772337 1101 96-97 0.7754446431185578 1101 98-99 0.7192366749257175 1101 100-101 0.548747505898806 1102 1 0.22257508169409235 1102 2 0.24468027805745152 1102 3 0.28761002347547304 1102 4 0.17631704120263691 1102 5 0.1324189043987829 1102 6 0.17651418344353687 1102 7 0.2800634869181309 1102 8 0.2393859828683773 1102 9 0.1647820038489911 1102 10-11 0.2951705610915738 1102 12-13 0.25407083488984483 1102 14-15 0.41505415692526526 1102 16-17 0.3600133562857053 1102 18-19 0.40669989346687174 1102 20-21 0.3731436747290502 1102 22-23 0.41961095431820894 1102 24-25 0.35361607225402025 1102 26-27 0.32941201218727656 1102 28-29 0.28544332129862937 1102 30-31 0.28656396339560786 1102 32-33 0.2828975262189104 1102 34-35 0.24590667580856262 1102 36-37 0.4562935026527484 1102 38-39 0.3329343406466947 1102 40-41 0.3419783663266074 1102 42-43 0.2626755871207038 1102 44-45 0.3241460350364136 1102 46-47 0.22589590447341124 1102 48-49 0.28664795514331587 1102 50-51 0.28078746653761755 1102 52-53 0.2636789620986164 1102 54-55 0.2835843314837092 1102 56-57 0.2837109720396427 1102 58-59 0.34699536970337874 1102 60-61 0.3720382800680113 1102 62-63 0.4013304600434182 1102 64-65 0.40375701709726286 1102 66-67 0.3413427720151745 1102 68-69 0.29320167733927605 1102 70-71 0.2665324622251646 1102 72-73 0.13149644158191265 1102 74-75 0.11224177176450212 1102 76-77 0.11237958348596777 1102 78-79 0.09785117904470297 1102 80-81 0.16708219353516185 1102 82-83 0.09106437286935432 1102 84-85 0.1436291743916449 1102 86-87 -0.00869474807806725 1102 88-89 0.059627200075834 1102 90-91 -0.1550905523860635 1102 92-93 0.06797269148915674 1102 94-95 0.12134195098581912 1102 96-97 -0.07752440998171295 1102 98-99 0.019753990834960433 1102 100-101 -0.11521756431251262 1103 1 0.29957557633753495 1103 2 0.3548701966822492 1103 3 0.35272744674519885 1103 4 0.26337956168790555 1103 5 0.24321016366795334 1103 6 0.29036693315035933 1103 7 0.32998399150718427 1103 8 0.2735932262418217 1103 9 0.3911349663507977 1103 10-11 0.42390085717511283 1103 12-13 0.4102133809153017 1103 14-15 0.4804545076114053 1103 16-17 0.5055933586881878 1103 18-19 0.5021955691131339 1103 20-21 0.4423541346035904 1103 22-23 0.5462424924291227 1103 24-25 0.5401276395496311 1103 26-27 0.4618270182738087 1103 28-29 0.4095788042089694 1103 30-31 0.5484749079152209 1103 32-33 0.6569860144058524 1103 34-35 0.5139748282277949 1103 36-37 0.46979813175288854 1103 38-39 0.5828688237682726 1103 40-41 0.49403674720013413 1103 42-43 0.5336905005125416 1103 44-45 0.48374994624420253 1103 46-47 0.4974814680780071 1103 48-49 0.6274273146771705 1103 50-51 0.5865880060658171 1103 52-53 0.6560437821363934 1103 54-55 0.5818860956320577 1103 56-57 0.5647707078477779 1103 58-59 0.621586317800336 1103 60-61 0.7831991312575397 1103 62-63 0.7040993603740802 1103 64-65 0.6043990705173172 1103 66-67 0.6456728682272725 1103 68-69 0.39184887668153934 1103 70-71 0.1467592165609659 1103 72-73 0.29970350416953195 1103 74-75 0.4316134762894954 1103 76-77 0.22583000081589688 1103 78-79 0.2706155908997445 1103 80-81 0.36517903911026295 1103 82-83 0.3120873904532928 1103 84-85 0.2505456467854863 1103 86-87 0.3773838083974823 1103 88-89 0.29530963419151135 1103 90-91 0.4324154208590727 1103 92-93 0.4113958039257106 1103 94-95 0.3230463608891405 1103 96-97 0.2999294159042769 1103 98-99 0.32836408352725854 1103 100-101 -0.021890359474742382 1104 1 0.26001855294150644 1104 2 0.2773939997361481 1104 3 0.2043594321968598 1104 4 0.17205851687347717 1104 5 0.14785093177586361 1104 6 0.15954971528869066 1104 7 0.08469100985170996 1104 8 0.1451893517932774 1104 9 0.2474972535342559 1104 10-11 0.25813707497755445 1104 12-13 0.23307656401563293 1104 14-15 0.36817539495813634 1104 16-17 0.3892423241741909 1104 18-19 0.3795098855512009 1104 20-21 0.321539657330149 1104 22-23 0.3434868658040884 1104 24-25 0.26040677310569293 1104 26-27 0.30110086690740445 1104 28-29 0.4024028758637499 1104 30-31 0.3276973008435675 1104 32-33 0.39790944167400966 1104 34-35 0.2854899554619337 1104 36-37 0.42343608843533787 1104 38-39 0.44382531228509237 1104 40-41 0.5295228358117114 1104 42-43 0.6165873138819649 1104 44-45 0.4594178194664522 1104 46-47 0.39318739599357144 1104 48-49 0.4136632909362419 1104 50-51 0.6593228692512341 1104 52-53 0.5943311931979238 1104 54-55 0.502757997518998 1104 56-57 0.5847383317461734 1104 58-59 0.5653576342929938 1104 60-61 0.4551478752900877 1104 62-63 0.5041659009272408 1104 64-65 0.4881436662747447 1104 66-67 0.43552520161751573 1104 68-69 0.478738645810207 1104 70-71 0.3327355336981199 1104 72-73 0.06499999284892866 1104 74-75 0.21356856083547981 1104 76-77 0.213916338635201 1104 78-79 0.08484952100777576 1104 80-81 0.13672064321371025 1104 82-83 0.14105145971585387 1104 84-85 0.2766439433240997 1104 86-87 0.2220348420985907 1104 88-89 0.21601669434161508 1104 90-91 0.20385814314574446 1104 92-93 0.2107025860686882 1104 94-95 0.3706882937470546 1104 96-97 0.34911263932376 1104 98-99 0.19603309711290962 1104 100-101 -0.06442614679580316 1105 1 0.18274019517888718 1105 2 0.18719056253466704 1105 3 0.16515050443024393 1105 4 0.11797659208600209 1105 5 0.11294941896501598 1105 6 0.1528142063271929 1105 7 0.1268631987362241 1105 8 0.1576393570372332 1105 9 0.19681057857174267 1105 10-11 0.1993798078447 1105 12-13 0.3060018702202285 1105 14-15 0.35839697341765486 1105 16-17 0.2650574760930624 1105 18-19 0.2821103215083056 1105 20-21 0.21109794604259946 1105 22-23 0.24599914658540456 1105 24-25 0.25555767152314957 1105 26-27 0.1856476330864325 1105 28-29 0.12243876153980437 1105 30-31 0.15671395962067436 1105 32-33 0.25959113629426867 1105 34-35 0.34049056489481444 1105 36-37 0.30768352385671704 1105 38-39 0.27402285357771206 1105 40-41 0.3573540767588028 1105 42-43 0.23300912080795655 1105 44-45 0.2732839766661215 1105 46-47 -0.005120931694236219 1105 48-49 -0.06981597559001784 1105 50-51 -0.07552365287117624 1105 52-53 0.049229191140021555 1105 54-55 0.014295837632161579 1105 56-57 0.10801640038248195 1105 58-59 0.1905666130818915 1105 60-61 -0.03259487033263042 1105 62-63 0.07992832981492626 1105 64-65 0.00260791807540528 1105 66-67 0.15411829036511193 1105 68-69 0.07605005467731374 1105 70-71 0.05447131194391375 1105 72-73 0.17285118917986253 1105 74-75 -0.03437311501992113 1105 76-77 0.16671902127373173 1105 78-79 0.20660145631151394 1105 80-81 0.33828957857697617 1105 82-83 0.45158075926487484 1105 84-85 0.3943038911112886 1105 86-87 0.34613777106138954 1105 88-89 0.2342582962147013 1105 90-91 0.26821964172704327 1105 92-93 0.26631886444629416 1105 94-95 0.2662623936997157 1105 96-97 -0.12850785122719444 1105 98-99 -0.24078486953552058 1105 100-101 -0.3782364629355719 1106 1 0.07936326668280103 1106 2 0.1090642081459876 1106 3 0.11318805275594457 1106 4 0.1130825251520804 1106 5 0.04807508671175498 1106 6 0.08873160402674785 1106 7 0.13293063690358053 1106 8 0.10852342945425164 1106 9 0.08095807439248404 1106 10-11 0.1308005096251179 1106 12-13 0.06662259230427026 1106 14-15 0.21816131745706002 1106 16-17 0.10028862230539914 1106 18-19 0.2038242712724454 1106 20-21 0.10982512049707083 1106 22-23 0.09374485814112177 1106 24-25 0.1435281063164311 1106 26-27 0.10502800427469339 1106 28-29 0.24514496747772085 1106 30-31 0.3271770329541539 1106 32-33 0.2895380400745964 1106 34-35 0.06759468179016892 1106 36-37 -0.3215385292305868 1106 38-39 0.06190247983477093 1106 40-41 0.21660859891792228 1106 42-43 0.32309888627315786 1106 44-45 0.14689952299827524 1106 46-47 -0.04594862807466882 1106 48-49 0.01412759147861209 1106 50-51 0.18476579654907965 1106 52-53 0.01786209316999532 1106 54-55 -0.148354410613166 1106 56-57 0.1734344124899181 1106 58-59 0.19968617746865647 1106 60-61 -0.15250029112254282 1106 62-63 -0.0811606028668308 1106 64-65 -0.40641922017835697 1106 66-67 -0.05241183625924606 1106 68-69 -0.9063233837142803 1106 70-71 -0.5561626576999288 1106 72-73 -0.4198357115772282 1106 74-75 -0.227497994048786 1106 76-77 -0.29876380626312127 1106 78-79 -0.21562220377450814 1106 80-81 -0.8668840537031173 1106 82-83 -0.3999583282878305 1106 84-85 -0.28689098858134443 1106 86-87 -0.24850006296536975 1106 88-89 -0.11351173507184953 1106 90-91 -0.12456241834381032 1106 92-93 -0.22611513342182477 1106 94-95 -0.10634533406209457 1106 96-97 -0.06806076979011877 1106 98-99 -0.19298382501423816 1106 100-101 -0.31430881525038146 1107 1 0.1319699735053348 1107 2 0.1654579663240483 1107 3 0.14776603768127927 1107 4 0.11095174103130745 1107 5 0.09612262491209833 1107 6 0.11538089625258863 1107 7 0.05830863913126194 1107 8 0.09983462299182833 1107 9 0.20573817236702752 1107 10-11 0.16752268799069014 1107 12-13 0.2117202964990028 1107 14-15 0.2656137089659367 1107 16-17 0.3057909310874294 1107 18-19 0.21164979872369827 1107 20-21 0.29702462738016067 1107 22-23 0.26251060598103493 1107 24-25 0.28938462659802866 1107 26-27 0.2696050159429646 1107 28-29 0.39677749805421314 1107 30-31 0.21361750747102803 1107 32-33 0.27030956257305405 1107 34-35 0.23797003459585397 1107 36-37 0.31462034957728946 1107 38-39 0.2261303625681066 1107 40-41 0.2752229512153477 1107 42-43 0.14357967526021298 1107 44-45 0.20040946644122926 1107 46-47 0.2793799002525219 1107 48-49 0.2650176350588964 1107 50-51 0.25260500366899663 1107 52-53 0.32024040900272865 1107 54-55 0.32849877896649105 1107 56-57 0.10506943027889548 1107 58-59 0.06685183663403649 1107 60-61 0.04937576999800086 1107 62-63 0.11375072260114649 1107 64-65 0.24752955405844546 1107 66-67 0.12485201372940224 1107 68-69 0.20283739835183567 1107 70-71 -0.0040403084736695405 1107 72-73 0.01925261690784552 1107 74-75 0.026560812305501003 1107 76-77 0.06584440370086853 1107 78-79 0.03236174829798699 1107 80-81 0.1615612762850951 1107 82-83 -0.11062738844593056 1107 84-85 0.2137234069719547 1107 86-87 0.23074836630587825 1107 88-89 0.16198278765370233 1107 90-91 0.16648275159768744 1107 92-93 -0.01626312256459883 1107 94-95 -0.18400973340027704 1107 96-97 -0.030213073705855464 1107 98-99 0.013854441290522601 1107 100-101 -0.2921680822796766 1108 1 0.13592453357392387 1108 2 0.18881023296213328 1108 3 0.12848514602979577 1108 4 0.11385389514126132 1108 5 0.03263532451556728 1108 6 0.17106838955448467 1108 7 0.15554794895871993 1108 8 0.0756635802311365 1108 9 0.11810233483621602 1108 10-11 0.20985791465450632 1108 12-13 0.16282521558592578 1108 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-0.4722951690365882 2316 70-71 -0.5516198489067214 2316 72-73 -0.26743098892512407 2316 74-75 0.057800317387251 2316 76-77 -0.07176165020986858 2316 78-79 -0.057765295449250686 2316 80-81 -0.26552113644137165 2316 82-83 -2.8713214897635453E-4 2316 84-85 -0.20865949183493981 2316 86-87 0.13330470744595502 2316 88-89 0.24433254461348852 2316 90-91 0.1695450582702449 2316 92-93 0.2617329004137474 2316 94-95 0.31616039474819857 2316 96-97 0.3609093301827855 2316 98-99 0.3848641628990066 2316 100-101 0.3980738532041954 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 5.0 4 40.0 5 91.0 6 150.0 7 483.0 8 1067.0 9 1889.0 10 3202.0 11 4882.0 12 6967.0 13 9891.0 14 13008.0 15 16811.0 16 21222.0 17 25875.0 18 25373.0 19 22800.0 20 22318.0 21 24319.0 22 26931.0 23 30263.0 24 35503.0 25 42015.0 26 50283.0 27 61248.0 28 72302.0 29 87528.0 30 103430.0 31 124234.0 32 149728.0 33 184238.0 34 234648.0 35 306948.0 36 411578.0 37 491085.0 38 259088.0 39 11222.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.80314646779486 19.611810060218666 15.104516278535701 23.480527193450772 2 15.758925162606987 20.64588120145218 39.405382516885446 24.18981111905539 3 19.25690571415389 23.964450753456283 29.84865656554093 26.929986966848894 4 13.321797747508043 17.120042016544364 36.56854711979225 32.98961311615535 5 13.986080268645399 37.17532084265551 34.29454195987262 14.544056928826468 6 32.628871665682624 36.330504247098354 17.097181297642397 13.943442789576622 7 29.075677652068876 32.084203763588945 20.446571481894935 18.39354710244724 8 26.250661712007673 35.83710818496993 19.422695733197717 18.489534369824685 9 26.059936017739084 16.796384190512004 19.89465336697802 37.24902642477089 10-11 24.90975392985818 26.77342287057087 27.45842922231234 20.858393977258615 12-13 25.708318040819044 23.729686395339883 27.901264589752433 22.66073097408864 14-15 22.43710180200744 25.361521975776657 25.82728744214573 26.37408878007017 16-17 22.43852409256128 28.588751277459746 26.556870461245357 22.415854168733613 18-19 22.326440658915992 27.250930906512494 28.683853949492725 21.738774485078785 20-21 22.779526925347785 26.526273869331046 28.772087998851063 21.922111206470106 22-23 22.57036348889954 26.358963934180558 28.600303051958008 22.470369524961892 24-25 22.51935228787067 26.55052894547918 28.700810134495125 22.22930863215502 26-27 22.24725903298354 26.905667021403 28.492717497174684 22.354356448438768 28-29 22.203742081137168 27.011188223156697 28.62052732983334 22.164542365872798 30-31 22.374156772496647 26.686767216827157 28.528824279124176 22.410251731552027 32-33 22.193283042064415 26.726521972307655 28.406576671520956 22.67361831410697 34-35 22.68976119090226 26.825119883318887 28.306167182327417 22.17895174345143 36-37 22.72960878796546 26.55772713451889 28.289143101559162 22.42352097595649 38-39 22.342478045111662 26.80113864124527 28.29208843463194 22.56429487901113 40-41 22.63727670847085 26.613354638717112 28.365446076558186 22.383922576253852 42-43 22.58406717395188 26.627322286842208 28.126716076963504 22.661894462242405 44-45 22.48939699737119 26.863113900496554 28.165342317584297 22.482146784547957 46-47 22.618981542831413 27.002741204867426 27.762469845705755 22.615807406595405 48-49 22.58318144889086 26.985933893322656 27.739106121607925 22.69177853617856 50-51 22.723832108660222 27.05944203194673 27.68093096895452 22.535794890438527 52-53 22.890153378425726 26.983800457491895 27.581717530320805 22.54432863376157 54-55 22.537182490978864 27.013009448865876 27.702369397302647 22.747438662852616 56-57 22.553729627422317 27.23480005023114 27.59512522054176 22.616345101804782 58-59 22.632909583254992 26.854875022288333 27.679127088252088 22.833088306204587 60-61 22.5258215115549 26.99786725796951 27.79648340395064 22.679827826524956 62-63 22.404753364410958 27.088980578449036 27.801478765895833 22.704787291244173 64-65 22.24084305058428 26.913865390259183 27.703375407694388 23.141916151462148 66-67 22.208583253416144 27.067184033976815 27.613864430962735 23.1103682816443 68-69 22.373154233898767 26.85785569465815 27.736198480249648 23.032791591193433 70-71 22.36909020519851 26.92978944534793 27.52567756581394 23.175442783639625 72-73 22.425151092353836 26.92347452400098 27.47457742360045 23.176796960044737 74-75 22.351868438817572 27.112049437432052 27.287459490736726 23.248622633013653 76-77 22.284153532449384 26.865715651509674 27.6191307098895 23.23100010615144 78-79 22.350255353189436 26.929822796472997 27.520611071525952 23.199310778811615 80-81 22.31336394659181 27.156200121486325 27.40638840447383 23.124047527448035 82-83 22.371426777448722 27.055247988227528 27.37590701911466 23.197418215209094 84-85 22.300964531616053 26.937345860715155 27.42899575686574 23.33269385080306 86-87 22.392610156438362 26.90321774922336 27.36505695369293 23.339115140645355 88-89 22.277949859696093 26.940570047709222 27.25116740689451 23.53031268570017 90-91 22.18459391347651 26.954242230445175 27.46749744976855 23.39366640630976 92-93 22.25845813881486 26.694095108584886 27.453760035245317 23.593686717354938 94-95 22.058142227394892 26.60413210310918 27.54086702530378 23.796858644192152 96-97 22.18631034934925 26.477777230446453 27.470085066851123 23.865827353353172 98-99 22.016782002389103 26.556251726913732 27.29414364418561 24.132822626511555 100-101 21.98285342012312 26.439271315452253 27.577537383693056 24.00033788073157 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 354.0 1 245.5 2 199.5 3 289.0 4 264.0 5 273.0 6 494.0 7 1059.5 8 1669.5 9 1903.5 10 1764.0 11 1597.5 12 1632.0 13 1795.5 14 2068.0 15 2239.0 16 2363.5 17 2569.5 18 2811.0 19 3171.5 20 3655.5 21 4225.0 22 5016.0 23 6077.0 24 7347.5 25 9260.5 26 12035.5 27 15651.0 28 19371.5 29 23849.0 30 29385.5 31 35254.5 32 42501.0 33 47959.0 34 52145.5 35 57973.0 36 64341.0 37 70359.5 38 75804.5 39 80256.0 40 85539.5 41 92779.0 42 99202.5 43 104687.5 44 109656.0 45 114249.5 46 115885.0 47 117043.0 48 120142.0 49 119922.0 50 116941.0 51 112516.0 52 107537.5 53 104406.0 54 101426.5 55 93051.0 56 81290.5 57 72570.0 58 64991.0 59 55388.5 60 45041.5 61 36869.0 62 30187.0 63 22765.0 64 16641.5 65 12617.0 66 9276.5 67 7171.0 68 5366.5 69 3737.5 70 2868.5 71 2279.0 72 1888.0 73 1553.0 74 1271.0 75 1044.5 76 737.0 77 434.5 78 258.5 79 163.5 80 95.5 81 50.5 82 28.5 83 19.0 84 11.5 85 7.5 86 4.0 87 2.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.5097017560778637E-4 2 0.05182688018138714 3 8.672503377072816E-4 4 0.0 5 0.08485177304128043 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5610506078731067E-4 26-27 0.008724538397335252 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009990723890387883 36-37 1.5610506078731067E-4 38-39 0.00400669656020764 40-41 0.0051167769924729605 42-43 3.1221012157462135E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.672503377072815E-5 68-69 0.001023355398494592 70-71 6.938002701658252E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0015610506078731067 82-83 5.897302296409514E-4 84-85 0.00468315182361932 86-87 0.002393610932072097 88-89 1.0407004052487377E-4 90-91 0.0032088262495169415 92-93 7.458352904282621E-4 94-95 5.203502026243688E-5 96-97 0.0 98-99 8.672503377072815E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2882674.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.15146948325443 #Duplication Level Percentage of deduplicated Percentage of total 1 71.99382754233696 36.82590072514531 2 14.677441983188103 15.015454513905674 3 5.442475531352643 8.351718631660308 4 2.6422023871314595 5.406101390957475 5 1.4443979507716782 3.694153885028637 6 0.886001264278718 2.719215997912661 7 0.5842800849971553 2.092074945618371 8 0.4071208695488134 1.6659864587777709 9 0.29434978114163207 1.355078146272193 >10 1.4881111968374157 13.498703957992774 >50 0.09268206254271501 3.2373085910131243 >100 0.04351471724528582 4.064798184465025 >500 0.0025094577211486374 0.8949638172086468 >1k 9.495245430393861E-4 0.7427675824686475 >5k 1.356463632913409E-4 0.43577317157350215 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6909 0.23967330332878434 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5652 0.1960679563488622 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4220 0.14639185700498913 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.938002701658252E-5 0.0 10-11 0.0 0.0 0.0 6.938002701658252E-5 0.0 12-13 0.0 0.0 0.0 6.938002701658252E-5 2.2548508780389318E-4 14-15 3.469001350829126E-5 0.0 0.0 6.938002701658252E-5 3.469001350829126E-4 16-17 3.469001350829126E-5 0.0 0.0 8.672503377072815E-5 3.469001350829126E-4 18-19 3.469001350829126E-5 0.0 0.0 1.0407004052487378E-4 3.469001350829126E-4 20-21 3.469001350829126E-5 0.0 0.0 1.0407004052487378E-4 3.8159014859120386E-4 22-23 3.469001350829126E-5 0.0 0.0 1.5610506078731067E-4 3.8159014859120386E-4 24-25 6.938002701658252E-5 0.0 0.0 4.6831518236193205E-4 3.8159014859120386E-4 26-27 6.938002701658252E-5 0.0 0.0 0.0010060103917404466 4.5097017560778637E-4 28-29 6.938002701658252E-5 0.0 0.0 0.002913961134696466 4.5097017560778637E-4 30-31 6.938002701658252E-5 0.0 0.0 0.00945302868100937 4.5097017560778637E-4 32-33 6.938002701658252E-5 0.0 0.0 0.025896095083939426 4.5097017560778637E-4 34-35 6.938002701658252E-5 0.0 0.0 0.05182688018138714 5.030051958702233E-4 36-37 6.938002701658252E-5 0.0 0.0 0.09555364220858828 5.203502026243689E-4 38-39 6.938002701658252E-5 0.0 0.0 0.16101369769873386 5.203502026243689E-4 40-41 6.938002701658252E-5 0.0 0.0 0.23124363004626955 5.203502026243689E-4 42-43 8.672503377072815E-5 0.0 0.0 0.3074402447172313 5.203502026243689E-4 44-45 1.3876005403316505E-4 0.0 0.0 0.4033234420541484 5.203502026243689E-4 46-47 1.3876005403316505E-4 0.0 0.0 0.5138805151050726 5.203502026243689E-4 48-49 1.3876005403316505E-4 0.0 0.0 0.6323469112358873 5.203502026243689E-4 50-51 1.3876005403316505E-4 0.0 0.0 0.7602489910409571 5.550402161326602E-4 52-53 1.3876005403316505E-4 0.0 0.0 0.8841790642993277 5.550402161326602E-4 54-55 1.3876005403316505E-4 0.0 0.0 1.0269284698859462 5.550402161326602E-4 56-57 1.3876005403316505E-4 0.0 0.0 1.1933017746717112 5.897302296409514E-4 58-59 1.3876005403316505E-4 0.0 0.0 1.3726664895163312 5.897302296409514E-4 60-61 1.3876005403316505E-4 0.0 0.0 1.5624555534201925 5.897302296409514E-4 62-63 1.3876005403316505E-4 0.0 0.0 1.7568236991071484 5.897302296409514E-4 64-65 1.3876005403316505E-4 0.0 0.0 1.9659524455418822 5.897302296409514E-4 66-67 1.3876005403316505E-4 0.0 0.0 2.2007171119592437 5.897302296409514E-4 68-69 1.734500675414563E-4 1.734500675414563E-5 0.0 2.4643438696155027 5.897302296409514E-4 70-71 1.9079507429560193E-4 3.469001350829126E-5 0.0 2.7519240815992374 5.897302296409514E-4 72-73 2.0814008104974756E-4 3.469001350829126E-5 0.0 3.0422968396703896 6.244202431492427E-4 74-75 2.0814008104974756E-4 3.469001350829126E-5 0.0 3.345158002604526 6.59110256657534E-4 76-77 2.0814008104974756E-4 3.469001350829126E-5 0.0 3.6657977974616625 6.59110256657534E-4 78-79 2.0814008104974756E-4 3.469001350829126E-5 0.0 4.022775381468733 6.59110256657534E-4 80-81 2.428300945580388E-4 3.469001350829126E-5 0.0 4.402162714202161 7.111452769199708E-4 82-83 2.428300945580388E-4 3.469001350829126E-5 0.0 4.798530808547897 7.458352904282621E-4 84-85 2.775201080663301E-4 3.469001350829126E-5 0.0 5.20246132583844 7.631802971824077E-4 86-87 2.948651148204757E-4 3.469001350829126E-5 0.0 5.621586069045615 7.805253039365533E-4 88-89 3.29555128328767E-4 3.469001350829126E-5 0.0 6.070700328930708 7.97870310690699E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4065 0.0 53.701546 1 GTATCAA 7535 0.0 42.730705 1 ATCAACG 9550 0.0 33.050125 3 TCAACGC 9830 0.0 32.10816 4 TATCAAC 9955 0.0 31.769289 2 CAACGCA 10030 0.0 31.467916 5 AACGCAG 10355 0.0 30.547157 6 ACGCAGA 11835 0.0 26.446234 7 CGCAGAG 11985 0.0 26.115242 8 GTACATG 9145 0.0 23.974627 1 TACATGG 9175 0.0 23.1183 2 GCAGAGT 13505 0.0 22.753927 9 GTATAGG 800 0.0 21.996134 1 ACATGGG 9265 0.0 21.599567 3 GAGTACT 7575 0.0 21.474577 12-13 TCGCGTA 155 3.912312E-5 21.449293 9 AGAGTAC 12140 0.0 21.145779 10-11 AGTACTT 8230 0.0 20.486849 12-13 TGGTATC 2120 0.0 19.965548 2 CAGAGTA 12985 0.0 19.733137 10-11 >>END_MODULE