FastQCFastQC Report
Thu 26 May 2016
SRR938409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889747
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69970.24213192365975292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54930.1900858448853827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.13575582914352016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41000.052.1923071
GTATCAA75650.042.272471
ATCAACG93950.033.486513
TCAACGC95800.032.9390644
TATCAAC97800.032.265462
CAACGCA97900.032.2325065
AACGCAG102000.031.0723556
ACGCAGA116150.026.9531427
CGCAGAG117750.026.385188
GTACATG95450.025.7143361
TACATGG94350.025.5372872
ACATGGG98450.023.5566083
GCAGAGT132550.022.7581639
GAGTACT73750.020.6436612-13
TGGTATC21100.020.4959322
AGAGTAC118700.020.41021710-11
CAGAGTA123250.020.31196210-11
CATGGGA43350.019.9522084
GTGGTAT22950.019.7280851
AGTACTT79050.019.37976512-13
Produced by FastQC (version 0.11.5)