FastQCFastQC Report
Thu 26 May 2016
SRR938409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889747
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69550.24067850922589418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57060.19745673237138062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42380.14665643739746073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.056.297211
GTATCAA71900.041.677831
ATCAACG92850.031.643853
TCAACGC93700.031.3060534
CAACGCA94800.030.9929495
TATCAAC96500.030.7081852
AACGCAG100050.029.4321026
ACGCAGA116250.025.1263187
CGCAGAG116050.025.0877698
TACATGG89600.024.1013432
GTACATG90900.023.8089891
ACATGGG92200.022.5337413
GCAGAGT133450.021.6743189
GAGTACT75650.020.8437512-13
GGACCGA8500.020.6742366
AGAGTAC120600.020.22273310-11
TAAGGTG12650.019.9190275
CATGGGG55500.019.7023324
CAGAGTA126450.019.62520410-11
AGTACTT81650.019.54473312-13
Produced by FastQC (version 0.11.5)