##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938409_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2889747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.814413164889523 31.0 31.0 34.0 30.0 34.0 2 32.0542133965361 33.0 31.0 34.0 30.0 34.0 3 32.12691422467088 33.0 31.0 34.0 30.0 34.0 4 35.78713257596599 37.0 35.0 37.0 35.0 37.0 5 35.65923591234804 37.0 35.0 37.0 33.0 37.0 6 35.69338942128844 37.0 35.0 37.0 33.0 37.0 7 35.629620863002884 37.0 35.0 37.0 33.0 37.0 8 35.614680454724926 37.0 35.0 37.0 33.0 37.0 9 37.19397511270018 39.0 37.0 39.0 33.0 39.0 10-11 37.128767847150634 39.0 37.0 39.0 33.0 39.0 12-13 37.021811771065074 39.0 37.0 39.0 33.0 39.0 14-15 38.088206337786666 40.0 37.0 41.0 33.0 41.0 16-17 38.00625885241857 40.0 37.0 41.0 33.0 41.0 18-19 37.99494090659148 40.0 37.0 41.0 33.0 41.0 20-21 37.992087715637396 40.0 37.0 41.0 33.0 41.0 22-23 38.025460706421704 40.0 37.0 41.0 33.0 41.0 24-25 37.95704502850941 40.0 37.0 41.0 33.0 41.0 26-27 37.79150441197793 40.0 37.0 41.0 32.0 41.0 28-29 37.66513642889845 40.0 37.0 41.0 32.0 41.0 30-31 37.437333787352316 39.0 36.5 41.0 32.0 41.0 32-33 37.242484549685486 39.0 36.0 41.0 31.0 41.0 34-35 37.078271731054656 39.0 36.0 41.0 31.0 41.0 36-37 37.097758904153196 39.0 36.0 41.0 31.0 41.0 38-39 36.968393599854934 39.0 36.0 41.0 31.0 41.0 40-41 36.8077978798836 39.0 35.5 41.0 30.0 41.0 42-43 36.607223919602646 39.0 35.0 40.5 30.0 41.0 44-45 36.34406074303391 39.0 35.0 40.0 30.0 41.0 46-47 36.00546120473523 38.5 35.0 40.0 29.5 41.0 48-49 35.646601415279605 38.0 35.0 40.0 28.0 41.0 50-51 35.30344957534345 38.0 34.0 40.0 27.0 41.0 52-53 34.80670072501157 38.0 33.5 40.0 26.0 41.0 54-55 34.495047663342156 38.0 33.0 40.0 25.5 41.0 56-57 34.13804668713213 37.0 33.0 40.0 24.0 41.0 58-59 33.68855543409164 37.0 32.5 40.0 23.0 41.0 60-61 33.287432083154684 36.5 32.0 39.0 22.5 41.0 62-63 32.81052545430448 36.0 31.0 39.0 21.5 40.0 64-65 32.31244015479556 35.5 31.0 39.0 19.5 40.0 66-67 31.8146249481356 35.0 30.5 38.0 18.5 40.0 68-69 31.24602032634691 35.0 29.5 38.0 15.5 40.0 70-71 30.666464053773566 34.0 29.0 37.0 12.0 39.0 72-73 30.06849959529329 34.0 28.5 36.5 8.0 39.0 74-75 29.43568727642939 34.0 28.0 36.0 7.0 39.0 76-77 28.13479432628531 31.5 26.5 34.5 4.0 36.5 78-79 28.75527061711631 33.0 28.0 35.0 2.0 37.0 80-81 28.42605148478396 33.0 27.0 35.0 2.0 37.0 82-83 27.908598399790705 33.0 26.0 35.0 2.0 36.0 84-85 27.304451739200697 32.5 26.0 35.0 2.0 36.0 86-87 26.641725728930595 32.0 25.0 35.0 2.0 35.5 88-89 25.886182596607938 32.0 23.5 34.0 2.0 35.0 90-91 25.322353998464223 31.0 20.5 34.0 2.0 35.0 92-93 24.532455263384648 31.0 18.0 34.0 2.0 35.0 94-95 23.72962027471609 31.0 6.0 34.0 2.0 35.0 96-97 22.728266696011794 30.0 2.0 34.0 2.0 35.0 98-99 21.543648630831697 30.0 2.0 34.0 2.0 35.0 100-101 18.37744411534989 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2920735635020151 1101 2 0.28460882316377933 1101 3 0.27690368173665547 1101 4 0.23820942924498922 1101 5 0.18031045542595336 1101 6 0.2695512731633727 1101 7 0.24597668285581165 1101 8 0.2752431134276634 1101 9 0.3162055791766889 1101 10-11 0.31913087735880197 1101 12-13 0.31777011901930763 1101 14-15 0.4393266775213789 1101 16-17 0.47070428553328014 1101 18-19 0.4988271931049155 1101 20-21 0.4676035933647569 1101 22-23 0.4095268066270492 1101 24-25 0.4907071700840575 1101 26-27 0.46771267344169587 1101 28-29 0.48711278367012056 1101 30-31 0.5280667693060082 1101 32-33 0.38814310606957036 1101 34-35 0.309138386088172 1101 36-37 0.5237921096808833 1101 38-39 0.7148463845578235 1101 40-41 0.8760384866893887 1101 42-43 0.7840017783032849 1101 44-45 0.7744344658126536 1101 46-47 0.7599543744724926 1101 48-49 0.846463425264794 1101 50-51 0.9943276311783222 1101 52-53 1.0002411882963358 1101 54-55 1.1388285870189634 1101 56-57 1.1992117234426658 1101 58-59 1.1277662088140232 1101 60-61 1.0731699677133975 1101 62-63 1.2470768984914926 1101 64-65 1.4089743443785565 1101 66-67 1.4431369047918068 1101 68-69 1.3099314062318363 1101 70-71 1.4472670921001445 1101 72-73 1.8030765794484047 1101 74-75 1.5562585209261393 1101 76-77 1.6486587087370772 1101 78-79 1.8984306748125555 1101 80-81 2.2525541359698096 1101 82-83 2.3236638671778707 1101 84-85 2.4606134985567003 1101 86-87 2.561456387999467 1101 88-89 2.7634194281012974 1101 90-91 3.025734142730208 1101 92-93 3.4222046768872048 1101 94-95 3.3442198456306436 1101 96-97 3.5958267849836254 1101 98-99 3.908792068000654 1101 100-101 4.229541952217328 1102 1 0.3966396106140735 1102 2 0.3830976429710944 1102 3 0.3158291975599212 1102 4 0.23111154659108735 1102 5 0.2758962300186738 1102 6 0.23864802120223771 1102 7 0.3894008663470956 1102 8 0.3296196947740242 1102 9 0.4657748676011906 1102 10-11 0.41449564488415547 1102 12-13 0.376521506929997 1102 14-15 0.6295900987655827 1102 16-17 0.47412706165355445 1102 18-19 0.5591819876007307 1102 20-21 0.5955026063991369 1102 22-23 0.562910880044484 1102 24-25 0.5375759875374442 1102 26-27 0.5530618166578876 1102 28-29 0.5020845334993354 1102 30-31 0.4292789765681633 1102 32-33 0.4138258211248882 1102 34-35 0.5030638987977838 1102 36-37 0.6330458854518071 1102 38-39 0.8213182984765481 1102 40-41 0.7420233879647995 1102 42-43 0.557340130844743 1102 44-45 0.5556397109474105 1102 46-47 0.5829597218596092 1102 48-49 0.704980791039894 1102 50-51 0.8719849936280397 1102 52-53 0.9124600332432848 1102 54-55 0.9308038322346803 1102 56-57 0.8898114191765174 1102 58-59 1.0997687420818423 1102 60-61 1.1824008270005848 1102 62-63 1.257387198890477 1102 64-65 1.2041218412197523 1102 66-67 1.2295341035007539 1102 68-69 1.3788255800272076 1102 70-71 1.5818767739408877 1102 72-73 1.5177511622656468 1102 74-75 1.6855124710616174 1102 76-77 1.616648366428329 1102 78-79 1.9591931709905488 1102 80-81 2.3106765660387296 1102 82-83 2.279612469490317 1102 84-85 2.5184472307489436 1102 86-87 2.709905135096765 1102 88-89 2.715936909704279 1102 90-91 2.8637685934289436 1102 92-93 3.232544868351937 1102 94-95 3.312547770549269 1102 96-97 3.664077117677415 1102 98-99 4.152973367278097 1102 100-101 4.573717646919949 1103 1 0.326615876775179 1103 2 0.32325273256098797 1103 3 0.3017604240695988 1103 4 0.2442391628963989 1103 5 0.1932663591174517 1103 6 0.19194189316779386 1103 7 0.2052945796119161 1103 8 0.2149119607260559 1103 9 0.3348389133298113 1103 10-11 0.3735792431146905 1103 12-13 0.40169636284017685 1103 14-15 0.5700751219767568 1103 16-17 0.6165777883984234 1103 18-19 0.5580331890223817 1103 20-21 0.5290829593419843 1103 22-23 0.5200116692718453 1103 24-25 0.3867463722679716 1103 26-27 0.4334095328401304 1103 28-29 0.494690991289211 1103 30-31 0.5594331277330014 1103 32-33 0.5838075634119875 1103 34-35 0.6429229093414079 1103 36-37 0.7698559054557066 1103 38-39 0.7855093048008186 1103 40-41 0.7132227664660817 1103 42-43 0.7468709895868173 1103 44-45 0.9630107428348609 1103 46-47 0.8594382164832624 1103 48-49 1.104561172491863 1103 50-51 1.0674253169214154 1103 52-53 1.2213504822143406 1103 54-55 1.2610319883125882 1103 56-57 1.2099289955215582 1103 58-59 1.4051852958999262 1103 60-61 1.364173939578329 1103 62-63 1.404991423466285 1103 64-65 1.5107561500400024 1103 66-67 1.5703444869958787 1103 68-69 1.661762399075105 1103 70-71 1.636894437713881 1103 72-73 1.67104527180717 1103 74-75 1.8419213796851501 1103 76-77 1.774785402620651 1103 78-79 1.9692426937004335 1103 80-81 2.305246826754594 1103 82-83 2.5720824992823452 1103 84-85 2.6872216119823413 1103 86-87 2.8624558100500828 1103 88-89 3.0677598894770632 1103 90-91 3.2214278049618343 1103 92-93 3.2891757917146833 1103 94-95 3.271124619246642 1103 96-97 3.810223873102462 1103 98-99 4.218182208430058 1103 100-101 4.254314461501409 1104 1 0.2992594139871514 1104 2 0.28595403901304906 1104 3 0.30973528349311863 1104 4 0.14670309791882374 1104 5 0.211581865892164 1104 6 0.1800679597306427 1104 7 0.21006881798113142 1104 8 0.27517870634557795 1104 9 0.3183376124728312 1104 10-11 0.3007468718249271 1104 12-13 0.39199518694006485 1104 14-15 0.5206485015006166 1104 16-17 0.5910165315561073 1104 18-19 0.5716882241315346 1104 20-21 0.6004793756908171 1104 22-23 0.576487745626082 1104 24-25 0.4578818012710215 1104 26-27 0.47872225139809643 1104 28-29 0.46470938512715776 1104 30-31 0.6721336182353497 1104 32-33 0.6504141217827026 1104 34-35 0.7129406037938821 1104 36-37 0.7807947605150574 1104 38-39 0.7613453035698967 1104 40-41 0.7126240925009384 1104 42-43 0.8991442255035267 1104 44-45 0.8386488155149934 1104 46-47 0.8177346766602582 1104 48-49 0.9103384461940394 1104 50-51 0.954222551084996 1104 52-53 1.0618106312300668 1104 54-55 1.2078137660572068 1104 56-57 1.381682914411492 1104 58-59 1.3490410779527693 1104 60-61 1.3779904161675702 1104 62-63 1.5470514167521827 1104 64-65 1.4992386700657363 1104 66-67 1.5853897838617002 1104 68-69 1.7977551068159023 1104 70-71 1.6853792190859842 1104 72-73 1.9129918480821537 1104 74-75 2.0236241734969873 1104 76-77 2.066106611718258 1104 78-79 2.3321068404035294 1104 80-81 2.635244518782528 1104 82-83 2.657711992834013 1104 84-85 2.707739133919734 1104 86-87 2.8375764540442248 1104 88-89 2.943484336263154 1104 90-91 3.0322971877308404 1104 92-93 3.367982896538578 1104 94-95 3.8021062300712636 1104 96-97 4.12172130402649 1104 98-99 4.470435511860774 1104 100-101 4.692582736443828 1105 1 0.23040946643686766 1105 2 0.24230943448573328 1105 3 0.250965976532342 1105 4 0.1487843063167631 1105 5 0.2091909025725016 1105 6 0.19115314959045548 1105 7 0.2538583225047759 1105 8 0.2052373602314148 1105 9 0.27381612486914975 1105 10-11 0.3584507443056779 1105 12-13 0.41612806590570983 1105 14-15 0.5952682580595479 1105 16-17 0.5051314238743245 1105 18-19 0.4451032245238338 1105 20-21 0.37732374402433777 1105 22-23 0.252985805235987 1105 24-25 0.2580481528160945 1105 26-27 0.41287801503921173 1105 28-29 0.4682817709520819 1105 30-31 0.5582737408402423 1105 32-33 0.5580022783508838 1105 34-35 0.5030972908039999 1105 36-37 0.5923139927582355 1105 38-39 0.6041395786821511 1105 40-41 0.5819171528568887 1105 42-43 0.6859497316126877 1105 44-45 0.6475584872609446 1105 46-47 0.6240201618530179 1105 48-49 0.6593327631209434 1105 50-51 0.804593916850223 1105 52-53 0.967673253334965 1105 54-55 0.8610186410463783 1105 56-57 0.8075910564307236 1105 58-59 1.1614766496093267 1105 60-61 1.1661505898480087 1105 62-63 1.213277109721112 1105 64-65 1.379737103377046 1105 66-67 1.4490300436567338 1105 68-69 1.4928161258143469 1105 70-71 1.4501811278656511 1105 72-73 1.5872826204240766 1105 74-75 1.8068727134991356 1105 76-77 1.7295366871524251 1105 78-79 2.0572539966674093 1105 80-81 2.389333805298577 1105 82-83 2.2789911355339783 1105 84-85 2.498606601897741 1105 86-87 2.8479870312450863 1105 88-89 2.957667188720489 1105 90-91 3.107992469614622 1105 92-93 3.5195572497380994 1105 94-95 3.533911756588868 1105 96-97 3.8557067931648845 1105 98-99 4.223860956844359 1105 100-101 4.707675919159772 1106 1 0.14875429176803578 1106 2 0.20937388186391814 1106 3 0.14978780265416702 1106 4 0.12786687204095415 1106 5 0.06858548023874533 1106 6 0.08957124577445796 1106 7 0.06942345131915317 1106 8 0.08211812184137557 1106 9 0.2208736874938353 1106 10-11 0.2416823049966439 1106 12-13 0.1599325825038207 1106 14-15 0.19461091396674846 1106 16-17 0.08960691783040176 1106 18-19 0.20060796187911478 1106 20-21 0.4047869167240208 1106 22-23 0.26406555802066123 1106 24-25 0.24848125194973392 1106 26-27 0.262478735667635 1106 28-29 0.3017764373117515 1106 30-31 0.3127012595087777 1106 32-33 0.25472820566950816 1106 34-35 0.21519754026277127 1106 36-37 0.30037807818730755 1106 38-39 0.27375224771851947 1106 40-41 0.2845583595598029 1106 42-43 0.3768093130687191 1106 44-45 0.04611203052476753 1106 46-47 -0.16154849108320235 1106 48-49 0.34276898173821024 1106 50-51 0.20201108976880278 1106 52-53 0.48117413686319566 1106 54-55 0.6649558708050449 1106 56-57 0.506084834947842 1106 58-59 0.9122987554810322 1106 60-61 0.6721588542770434 1106 62-63 0.8437025429814611 1106 64-65 0.4979231388625891 1106 66-67 0.8503725705883411 1106 68-69 0.7500690728385315 1106 70-71 0.9089022394899366 1106 72-73 1.31854482825916 1106 74-75 1.6061946379421457 1106 76-77 1.5283570146141905 1106 78-79 1.830681913616207 1106 80-81 1.7160902916582756 1106 82-83 1.9870281996679964 1106 84-85 2.0504520655218563 1106 86-87 2.336868108936077 1106 88-89 2.5962817774041547 1106 90-91 2.6718307333124898 1106 92-93 2.471946636136714 1106 94-95 3.0003023746135717 1106 96-97 3.570023015887571 1106 98-99 3.88419907726124 1106 100-101 4.3967981902928095 1107 1 0.10136355713451195 1107 2 0.12569003291978476 1107 3 0.18722162321422076 1107 4 0.17388470748628748 1107 5 0.0907762684792246 1107 6 -0.012604110429101922 1107 7 0.08866407577790625 1107 8 0.18034931476988447 1107 9 0.16508282231578875 1107 10-11 0.12215726088443546 1107 12-13 0.20242043968134737 1107 14-15 0.2931575598139631 1107 16-17 0.3324306422051464 1107 18-19 0.3131102270403332 1107 20-21 0.2890854870121444 1107 22-23 0.2620029812444997 1107 24-25 0.25125788323313003 1107 26-27 0.29949714680897443 1107 28-29 0.19018402118282296 1107 30-31 0.21322138950120717 1107 32-33 0.2628298024356326 1107 34-35 0.3121315248939638 1107 36-37 0.43537311598155526 1107 38-39 0.38366365820936466 1107 40-41 0.39936173911355155 1107 42-43 0.4861623919292768 1107 44-45 0.48671894680218486 1107 46-47 0.39161489839051455 1107 48-49 0.4569462417902912 1107 50-51 0.4083836394431728 1107 52-53 0.673571476640042 1107 54-55 0.5803144207812849 1107 56-57 0.5833967352923182 1107 58-59 0.734397445716958 1107 60-61 0.8958558244597299 1107 62-63 0.9402236783270155 1107 64-65 1.0466432504220577 1107 66-67 1.08449351216521 1107 68-69 1.0408104740193984 1107 70-71 1.126002080672638 1107 72-73 1.3392930371678027 1107 74-75 1.2552982351739956 1107 76-77 1.378150000027663 1107 78-79 1.528417353471557 1107 80-81 1.7723292914343176 1107 82-83 1.9184480227561238 1107 84-85 2.144181987956504 1107 86-87 2.314528387736793 1107 88-89 2.5378545317110763 1107 90-91 2.770986245590663 1107 92-93 2.832901913060745 1107 94-95 3.0592152664196774 1107 96-97 3.4607696709067426 1107 98-99 3.5568328575715427 1107 100-101 3.894015659572325 1108 1 0.10198642449662287 1108 2 0.1443663448194954 1108 3 0.06415922829241083 1108 4 0.09546615470227948 1108 5 -1.2822497853193227E-4 1108 6 -0.0025818993075787944 1108 7 0.11339952785606044 1108 8 0.09860976294844903 1108 9 0.1439645460131942 1108 10-11 0.11374417712765705 1108 12-13 0.2251964948589844 1108 14-15 0.24607292688427407 1108 16-17 0.32420864365658275 1108 18-19 0.24084758399582995 1108 20-21 0.22677682561364065 1108 22-23 0.1761728083033134 1108 24-25 0.2562229512353511 1108 26-27 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1.8551945846369158 2316 86-87 1.9259470404764052 2316 88-89 2.0659936638808425 2316 90-91 2.337692277585777 2316 92-93 2.2705816212899634 2316 94-95 2.912893307014947 2316 96-97 3.068301446838035 2316 98-99 3.1657787632740586 2316 100-101 3.591379811553953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 13.0 4 30.0 5 105.0 6 174.0 7 462.0 8 1038.0 9 1942.0 10 3236.0 11 4991.0 12 7234.0 13 10357.0 14 13635.0 15 17896.0 16 22340.0 17 27795.0 18 27581.0 19 25057.0 20 26140.0 21 27791.0 22 31106.0 23 35258.0 24 40753.0 25 47923.0 26 56063.0 27 66579.0 28 80269.0 29 94517.0 30 112083.0 31 131321.0 32 157202.0 33 190735.0 34 236875.0 35 298412.0 36 379851.0 37 442118.0 38 256387.0 39 14470.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.880774296426054 19.56019664011993 15.104510915492227 23.454518147961792 2 15.82055437478167 20.575874521838507 39.43602823286331 24.167542870516517 3 19.26218275692623 23.9567919605224 29.811990006021304 26.969035276530068 4 13.319366712726064 17.167904318267308 36.48295162171637 33.02977734729026 5 14.043856373563413 37.15179512262035 34.3047056370238 14.499642866792431 6 32.60987899632736 36.33584531794652 17.107622224367738 13.946653461358382 7 29.09386185018965 32.05536678470468 20.46566706358723 18.38510430151844 8 26.23440737199485 35.83154511450311 19.447100386296793 18.48694712720525 9 26.016775863077285 16.84663051817339 19.875580803440577 37.261012815308746 10-11 24.92354867052375 26.77416050609275 27.454444973902557 20.847845849480944 12-13 25.72194036363737 23.690015077444496 27.882925391046342 22.70511916787179 14-15 22.41893494482389 25.381374217189258 25.843975268423154 26.355715569563703 16-17 22.414107532597143 28.62376879360027 26.545074707232157 22.41704896657043 18-19 22.33643637314962 27.230273100032633 28.668928456366594 21.764362070451153 20-21 22.759518393824788 26.563536531052716 28.73922872832812 21.93771634679437 22-23 22.55266637529168 26.384766555688095 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22.619344852340802 48-49 22.543253786577164 26.955993033300146 27.778815930944823 22.72193724917787 50-51 22.674026480518883 27.07018988167476 27.691559157254943 22.564224480551413 52-53 22.883577697286302 27.040464095991794 27.55012809079826 22.525830115923643 54-55 22.582897842423204 27.034724326164945 27.66654445294769 22.71583337846417 56-57 22.572771941626723 27.19603134807303 27.604510014198475 22.626686696101768 58-59 22.5960957827796 26.866279297114936 27.70175036084474 22.835874559260724 60-61 22.484736553061566 26.9944047004807 27.821795472060355 22.69906327439738 62-63 22.416651007856398 27.09327148708866 27.80618856944916 22.683888935605783 64-65 22.26838543305002 26.915245521493752 27.712841297179303 23.103527748276925 66-67 22.24997365680413 27.074282275519202 27.59642258534979 23.079321482326872 68-69 22.418958525500308 26.85293951236781 27.7254230009314 23.00267896120048 70-71 22.40426238006303 26.89808138913199 27.48925771010403 23.20839852070095 72-73 22.454353270372803 26.917719786541866 27.43757498493813 23.1903519581472 74-75 22.361473167028116 27.110920090928374 27.232816575291885 23.29479016675162 76-77 22.32325182792819 26.867680803890444 27.581151567940033 23.227915800241338 78-79 22.372339170176488 26.972638089078387 27.529555355538044 23.12546738520708 80-81 22.312501221993426 27.164710525546283 27.417898911859197 23.1048893406011 82-83 22.40384162850831 27.059322866777038 27.402921121551216 23.133914383163443 84-85 22.324690556090612 26.96315673450375 27.402176549918867 23.30997615948677 86-87 22.380710141692663 26.916242315392058 27.374541691972016 23.328505850943262 88-89 22.28985988207608 26.976277660533654 27.212665341174624 23.52119711621564 90-91 22.207474534284223 26.958075674153037 27.44563181500205 23.388817976560695 92-93 22.249658360385933 26.724817206801575 27.452096918028296 23.5734275147842 94-95 22.051567144113307 26.65961760666245 27.531268308263662 23.757546940960577 96-97 22.176837626269705 26.5143626760405 27.50730427265778 23.80149542503202 98-99 22.01909025253768 26.59154936401007 27.29259689516072 24.096763488291536 100-101 21.997137444865015 26.40343755774769 27.621200072255984 23.97822492513131 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 370.0 1 247.5 2 184.0 3 287.5 4 283.5 5 272.0 6 490.0 7 1104.5 8 1718.0 9 1979.5 10 1823.0 11 1624.0 12 1701.0 13 1860.0 14 2095.0 15 2224.5 16 2385.5 17 2593.0 18 2903.0 19 3199.0 20 3590.0 21 4288.5 22 5071.0 23 6149.0 24 7497.0 25 9480.5 26 12319.5 27 15871.0 28 19486.0 29 23844.0 30 29546.0 31 35470.0 32 42682.5 33 48332.0 34 52582.0 35 58092.5 36 64312.5 37 70737.0 38 76345.5 39 80815.5 40 85763.5 41 92914.5 42 99491.0 43 104822.0 44 109523.0 45 114014.0 46 116225.5 47 117376.0 48 119692.5 49 119424.0 50 116684.0 51 112625.0 52 107363.5 53 103624.0 54 101012.0 55 93038.0 56 81608.0 57 72574.0 58 64986.0 59 55759.0 60 45135.5 61 36958.0 62 30536.0 63 23251.5 64 17210.0 65 13045.5 66 9492.5 67 7272.5 68 5438.0 69 3869.0 70 3047.0 71 2392.5 72 1909.5 73 1626.5 74 1346.0 75 1114.0 76 799.0 77 460.5 78 266.0 79 153.5 80 94.5 81 60.0 82 34.5 83 19.5 84 10.0 85 7.0 86 4.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.46051055680653E-5 2 0.046370841461207506 3 2.422357389764571E-4 4 0.0 5 0.09928204787477936 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012284812476663182 26-27 0.011142843992917028 28-29 0.0 30-31 0.0 32-33 4.1526126681678356E-4 34-35 0.01475907752477985 36-37 0.0030452492899897465 38-39 0.01200797163211866 40-41 0.007163256852589518 42-43 0.005017740307369468 44-45 0.00261268547038893 46-47 5.1907658352097945E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.730255278403265E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2493318619242448E-4 68-69 2.5953829176048976E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.1907658352097945E-5 82-83 0.0012111786948822854 84-85 0.0044294535127123585 86-87 0.0014534144338587426 88-89 8.651276392016325E-5 90-91 9.86245508689861E-4 92-93 2.0763063340839178E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.267072169293713E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2889747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.1154565016554 #Duplication Level Percentage of deduplicated Percentage of total 1 72.00903418295387 36.80774654504995 2 14.673215076665603 15.000561739814696 3 5.428168309270996 8.323899032886176 4 2.6571039974785635 5.432763352139607 5 1.4195575889750836 3.6280667095425345 6 0.8691256733025192 2.665545332890013 7 0.6000629022789539 2.1470742425787877 8 0.4122392515400398 1.6857438024295905 9 0.30137898770645544 1.3864612082960033 >10 1.489967478657015 13.507433525586551 >50 0.09234863219996235 3.2002489721520875 >100 0.04413990508070801 4.104651397663316 >500 0.0024386759270090368 0.8533261099329184 >1k 0.001083855967395787 0.8180767193223417 >5k 1.3548199592447338E-4 0.43840130971557467 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6955 0.24067850922589418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5706 0.19745673237138062 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4238 0.14665643739746073 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.46051055680653E-5 2 3.46051055680653E-5 0.0 0.0 0.0 3.46051055680653E-5 3 3.46051055680653E-5 0.0 0.0 0.0 3.46051055680653E-5 4 3.46051055680653E-5 0.0 0.0 0.0 3.46051055680653E-5 5 3.46051055680653E-5 0.0 0.0 0.0 3.46051055680653E-5 6 3.46051055680653E-5 0.0 0.0 0.0 6.92102111361306E-5 7 3.46051055680653E-5 0.0 0.0 0.0 6.92102111361306E-5 8 3.46051055680653E-5 0.0 0.0 0.0 6.92102111361306E-5 9 3.46051055680653E-5 0.0 0.0 0.0 6.92102111361306E-5 10-11 6.92102111361306E-5 0.0 0.0 0.0 6.92102111361306E-5 12-13 6.92102111361306E-5 0.0 0.0 3.46051055680653E-5 2.5953829176048976E-4 14-15 6.92102111361306E-5 0.0 0.0 3.46051055680653E-5 2.768408445445224E-4 16-17 6.92102111361306E-5 0.0 0.0 8.651276392016325E-5 2.768408445445224E-4 18-19 8.651276392016325E-5 0.0 0.0 1.038153167041959E-4 2.768408445445224E-4 20-21 1.038153167041959E-4 0.0 0.0 1.2111786948822855E-4 3.2874850289662036E-4 22-23 1.038153167041959E-4 0.0 0.0 3.6335360846468566E-4 3.806561612487183E-4 24-25 1.730255278403265E-4 0.0 0.0 6.40194453009208E-4 3.806561612487183E-4 26-27 1.730255278403265E-4 0.0 0.0 0.0010554557198259916 3.9795871403275096E-4 28-29 1.730255278403265E-4 0.0 0.0 0.003010644184421681 4.152612668167836E-4 30-31 1.730255278403265E-4 0.0 0.0 0.010658372514964112 4.152612668167836E-4 32-33 1.730255278403265E-4 0.0 0.0 0.027459151268259818 4.498663723848489E-4 34-35 1.730255278403265E-4 0.0 0.0 0.052911206413571846 4.844714779529142E-4 36-37 2.076306334083918E-4 0.0 0.0 0.09580423476518879 4.844714779529142E-4 38-39 2.076306334083918E-4 0.0 0.0 0.1618826838474095 4.844714779529142E-4 40-41 2.076306334083918E-4 0.0 0.0 0.2311101975363241 4.844714779529142E-4 42-43 2.422357389764571E-4 0.0 0.0 0.30686077362481906 4.844714779529142E-4 44-45 3.114459501125877E-4 0.0 0.0 0.3971800991574695 4.844714779529142E-4 46-47 3.114459501125877E-4 0.0 0.0 0.5096120871481137 4.844714779529142E-4 48-49 3.114459501125877E-4 0.0 0.0 0.6280134558492492 4.844714779529142E-4 50-51 3.114459501125877E-4 0.0 0.0 0.7561215566622268 5.190765835209795E-4 52-53 3.114459501125877E-4 0.0 0.0 0.8818765102965761 5.536816890890448E-4 54-55 3.114459501125877E-4 0.0 0.0 1.020123907040997 5.536816890890448E-4 56-57 3.46051055680653E-4 0.0 0.0 1.1835984257445373 5.882867946571101E-4 58-59 3.46051055680653E-4 0.0 0.0 1.3647907584989274 6.228919002251754E-4 60-61 3.46051055680653E-4 0.0 0.0 1.5552399569927748 6.40194453009208E-4 62-63 3.46051055680653E-4 0.0 0.0 1.747765461820706 6.574970057932407E-4 64-65 3.46051055680653E-4 0.0 0.0 1.9555864233097222 6.747995585772734E-4 66-67 3.46051055680653E-4 0.0 0.0 2.1961784197716963 6.92102111361306E-4 68-69 3.806561612487183E-4 0.0 0.0 2.457204731071613 6.92102111361306E-4 70-71 4.152612668167836E-4 0.0 0.0 2.736139184503003 6.92102111361306E-4 72-73 4.498663723848489E-4 0.0 0.0 3.0234134683762974 6.92102111361306E-4 74-75 4.498663723848489E-4 1.730255278403265E-5 0.0 3.32252269835387 6.92102111361306E-4 76-77 4.498663723848489E-4 3.46051055680653E-5 0.0 3.6483643723827726 7.267072169293713E-4 78-79 4.498663723848489E-4 3.46051055680653E-5 0.0 4.00962437196059 7.267072169293713E-4 80-81 4.844714779529142E-4 3.46051055680653E-5 0.0 4.393896766741172 7.267072169293713E-4 82-83 4.844714779529142E-4 3.46051055680653E-5 0.0 4.787356817050074 7.267072169293713E-4 84-85 5.017740307369468E-4 3.46051055680653E-5 0.0 5.1887933441924154 7.267072169293713E-4 86-87 5.190765835209795E-4 3.46051055680653E-5 0.0 5.610716093831051 7.959174280655019E-4 88-89 5.363791363050122E-4 3.46051055680653E-5 0.0 6.069441373241325 8.651276392016325E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3895 0.0 56.29721 1 GTATCAA 7190 0.0 41.67783 1 ATCAACG 9285 0.0 31.64385 3 TCAACGC 9370 0.0 31.306053 4 CAACGCA 9480 0.0 30.992949 5 TATCAAC 9650 0.0 30.708185 2 AACGCAG 10005 0.0 29.432102 6 ACGCAGA 11625 0.0 25.126318 7 CGCAGAG 11605 0.0 25.087769 8 TACATGG 8960 0.0 24.101343 2 GTACATG 9090 0.0 23.808989 1 ACATGGG 9220 0.0 22.533741 3 GCAGAGT 13345 0.0 21.674318 9 GAGTACT 7565 0.0 20.84375 12-13 GGACCGA 850 0.0 20.674236 6 AGAGTAC 12060 0.0 20.222733 10-11 TAAGGTG 1265 0.0 19.919027 5 CATGGGG 5550 0.0 19.702332 4 CAGAGTA 12645 0.0 19.625204 10-11 AGTACTT 8165 0.0 19.544733 12-13 >>END_MODULE