FastQCFastQC Report
Thu 26 May 2016
SRR938408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918210
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64270.2202377484828028No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51580.1767521871284109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.12380877318630257No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.054.9131131
GTATCAA74450.044.794571
ATCAACG93700.034.8965723
TATCAAC94400.034.736712
TCAACGC95750.034.199074
CAACGCA97100.033.6746525
AACGCAG101200.032.256216
ACGCAGA117950.027.630987
CGCAGAG120100.026.9781068
GTACATG92900.024.7539351
GCAGAGT130350.024.0900769
TACATGG92900.023.7875022
GGACCGA7000.023.0790526
ACATGGG98450.021.7718223
GAGTACT73500.021.6510612-13
GTACTAG5900.021.0249181
TGGTATC19800.020.8816912
GTGGTAT23750.020.691241
AGAGTAC120250.020.24507110-11
CAGAGTA126300.020.19674910-11
Produced by FastQC (version 0.11.5)