##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938408_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2918210 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.089471970831433 31.0 31.0 34.0 28.0 34.0 2 31.256995555494637 31.0 31.0 34.0 28.0 34.0 3 31.311794901669174 31.0 31.0 34.0 27.0 34.0 4 34.974076917014195 37.0 35.0 37.0 32.0 37.0 5 34.96778401828518 37.0 35.0 37.0 32.0 37.0 6 34.937872188773255 37.0 35.0 37.0 32.0 37.0 7 34.852948211403564 37.0 35.0 37.0 32.0 37.0 8 34.80930570452435 37.0 35.0 37.0 32.0 37.0 9 36.29080360906172 39.0 35.0 39.0 32.0 39.0 10-11 36.12889288296593 39.0 35.0 39.0 31.0 39.0 12-13 35.94584933914969 38.0 35.0 39.0 30.0 39.0 14-15 37.1143678830516 39.0 36.0 41.0 31.0 41.0 16-17 37.04297274699216 39.0 36.0 41.0 31.0 41.0 18-19 37.02596094866374 39.0 36.0 41.0 31.0 41.0 20-21 36.95586232656321 39.0 36.0 41.0 30.5 41.0 22-23 36.79954269912035 39.0 36.0 41.0 30.0 41.0 24-25 36.66035583456983 39.0 36.0 41.0 30.0 41.0 26-27 36.45238331031695 39.0 36.0 41.0 29.5 41.0 28-29 36.21883140692411 39.0 35.0 40.0 29.0 41.0 30-31 35.95767131220851 38.5 35.0 40.0 27.5 41.0 32-33 35.68037272848767 38.0 34.5 40.0 27.0 41.0 34-35 35.37631304806714 38.0 34.0 40.0 26.5 41.0 36-37 35.173680783768134 38.0 34.0 40.0 25.5 41.0 38-39 34.94224730228462 38.0 34.0 40.0 25.0 41.0 40-41 34.67388381919053 38.0 33.5 40.0 24.5 41.0 42-43 34.276947855020715 38.0 33.0 40.0 24.0 41.0 44-45 33.82679604963316 38.0 33.0 40.0 22.5 41.0 46-47 33.36377025642432 37.5 32.0 40.0 19.5 41.0 48-49 32.901111811692786 37.0 31.0 40.0 16.5 41.0 50-51 32.188763145901085 36.5 30.5 39.0 15.0 40.5 52-53 32.20012970279726 36.5 31.0 39.0 13.5 40.0 54-55 32.176552749802106 37.0 31.0 39.0 10.0 40.5 56-57 31.745503750586835 36.0 30.0 39.0 8.0 40.5 58-59 31.286666141230413 36.0 29.5 39.0 4.5 40.0 60-61 30.731874676599695 35.0 28.5 39.0 2.0 40.0 62-63 30.001242371179593 35.0 28.0 38.0 2.0 40.0 64-65 29.336853070889347 34.0 26.5 38.0 2.0 40.0 66-67 28.6323777247011 34.0 26.0 37.5 2.0 40.0 68-69 27.770395036683446 33.5 24.5 37.0 2.0 39.0 70-71 26.914885323537373 32.5 22.0 36.0 2.0 39.0 72-73 25.99901960448357 32.0 19.0 35.5 2.0 38.5 74-75 25.03982355622111 31.0 15.5 35.0 2.0 38.0 76-77 24.061563766829664 30.5 7.5 34.5 2.0 37.0 78-79 23.047499494553165 29.5 2.0 34.0 2.0 36.0 80-81 22.059669454905574 29.0 2.0 34.0 2.0 36.0 82-83 21.034273235990554 28.0 2.0 34.0 2.0 35.0 84-85 20.018501581448902 26.0 2.0 33.0 2.0 35.0 86-87 19.0031332563455 25.5 2.0 32.5 2.0 35.0 88-89 17.9002160571035 22.5 2.0 32.0 2.0 34.0 90-91 16.723527950353127 19.0 2.0 31.5 2.0 34.0 92-93 15.549472793253397 4.5 2.0 31.0 2.0 34.0 94-95 14.280663488919577 2.0 2.0 30.5 2.0 34.0 96-97 12.851246825965232 2.0 2.0 29.5 2.0 34.0 98-99 11.147084685475 2.0 2.0 28.0 2.0 34.0 100-101 7.850055342144671 2.0 2.0 9.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4696937753570758 1101 2 0.3439545451469783 1101 3 0.3956958841009275 1101 4 0.24593271473690237 1101 5 0.30137688314458444 1101 6 0.3538261807458696 1101 7 0.2832063082957532 1101 8 0.32758020228032336 1101 9 0.4404216170607782 1101 10-11 0.5366367064536135 1101 12-13 0.47801382338258236 1101 14-15 0.5333585487376951 1101 16-17 0.5954460080578485 1101 18-19 0.6091874623796514 1101 20-21 0.6557997214932882 1101 22-23 0.6476677808817684 1101 24-25 0.6229600636010275 1101 26-27 0.7335390386171738 1101 28-29 0.8485493308717196 1101 30-31 0.9746005716627408 1101 32-33 1.0024381455420368 1101 34-35 1.011188396351912 1101 36-37 1.0245845404500713 1101 38-39 1.1333697384245411 1101 40-41 1.132538665269621 1101 42-43 1.372845335958317 1101 44-45 1.3676704526381727 1101 46-47 1.3946276482923352 1101 48-49 1.2469068438541555 1101 50-51 1.221749395057941 1101 52-53 1.203164720551591 1101 54-55 1.5329374691497506 1101 56-57 1.5523788831133771 1101 58-59 1.6758567156899886 1101 60-61 1.757151895486576 1101 62-63 1.751438102390754 1101 64-65 1.7657761681236401 1101 66-67 2.0754000140830193 1101 68-69 2.247169906262954 1101 70-71 2.4895682165622013 1101 72-73 2.75070183983739 1101 74-75 2.6368505469600088 1101 76-77 2.9306481697082454 1101 78-79 3.0301059001956823 1101 80-81 3.329982742266509 1101 82-83 3.5750186042435566 1101 84-85 3.6977381653192616 1101 86-87 3.7477161540226547 1101 88-89 4.170814379106531 1101 90-91 4.373471192689223 1101 92-93 4.409282473601291 1101 94-95 4.113112702401674 1101 96-97 4.128585844432813 1101 98-99 3.6618831121629505 1101 100-101 2.537311502329172 1102 1 0.6088597887442795 1102 2 0.5027247314823811 1102 3 0.5227775942860582 1102 4 0.2580904666515096 1102 5 0.3050311073500964 1102 6 0.36175309384965004 1102 7 0.44538613100576185 1102 8 0.4421056801793739 1102 9 0.550914049069462 1102 10-11 0.5646723453609255 1102 12-13 0.5948662626275905 1102 14-15 0.6627366467899094 1102 16-17 0.7002551499501521 1102 18-19 0.801302580271468 1102 20-21 0.7344513526818517 1102 22-23 0.6449206904879858 1102 24-25 0.6497875978228151 1102 26-27 0.7265175105462518 1102 28-29 0.6684392181056324 1102 30-31 0.5619024470773226 1102 32-33 0.6951982010205384 1102 34-35 0.7856024951277618 1102 36-37 0.779982598702496 1102 38-39 0.9275210379481464 1102 40-41 0.9870748167500523 1102 42-43 1.1433979500812086 1102 44-45 1.1088119831212069 1102 46-47 1.2239634741380812 1102 48-49 1.251369787832921 1102 50-51 1.4211166386206244 1102 52-53 1.3776358053975173 1102 54-55 1.287496354755497 1102 56-57 1.6319270348350159 1102 58-59 1.8773144736348684 1102 60-61 1.752313808288843 1102 62-63 1.702859256584805 1102 64-65 1.9699957303258948 1102 66-67 2.287384938403104 1102 68-69 2.283449107854942 1102 70-71 2.1432321495055753 1102 72-73 2.3332604842322517 1102 74-75 2.264610542135788 1102 76-77 2.5800264486686295 1102 78-79 3.0498023363049533 1102 80-81 3.21383644786863 1102 82-83 3.339555599962061 1102 84-85 3.4155045525833287 1102 86-87 3.5833961274894577 1102 88-89 3.9846669389569804 1102 90-91 3.886422468091741 1102 92-93 3.9336305408991237 1102 94-95 3.8658245760071726 1102 96-97 3.751326120619268 1102 98-99 3.4861437405172886 1102 100-101 2.625845121095378 1103 1 0.5635836430333541 1103 2 0.533095471787906 1103 3 0.4410654908783016 1103 4 0.39137307534716115 1103 5 0.4226063129905242 1103 6 0.5026238341551732 1103 7 0.5005377173747334 1103 8 0.3932402277704412 1103 9 0.5332295602327974 1103 10-11 0.6342610645148667 1103 12-13 0.5772158513467431 1103 14-15 0.6413497490225808 1103 16-17 0.7796690747445112 1103 18-19 0.7988025802714702 1103 20-21 0.7502298485690488 1103 22-23 0.6607961311460357 1103 24-25 0.7315067517593619 1103 26-27 0.7553935387483719 1103 28-29 0.8425455635815524 1103 30-31 0.8302702496625187 1103 32-33 1.091718471290811 1103 34-35 1.0199541402511514 1103 36-37 1.0643001662700655 1103 38-39 1.0533750332477965 1103 40-41 1.2005886240356034 1103 42-43 1.2612557644172853 1103 44-45 1.4583639807710256 1103 46-47 1.5599100076280905 1103 48-49 1.4453057455297582 1103 50-51 1.464289082803937 1103 52-53 1.598456310685414 1103 54-55 1.7335524652137835 1103 56-57 1.7210248609219718 1103 58-59 1.9282583631765817 1103 60-61 2.08240047103855 1103 62-63 2.138524356467297 1103 64-65 2.1390691733341214 1103 66-67 2.289750390812035 1103 68-69 2.3680968751874936 1103 70-71 2.409516814605457 1103 72-73 2.7483721176047524 1103 74-75 2.630335571512994 1103 76-77 2.908119868175092 1103 78-79 3.1653096806057732 1103 80-81 3.17061582507192 1103 82-83 3.2424798067775704 1103 84-85 3.652957549058062 1103 86-87 3.694316221496507 1103 88-89 3.759579982435241 1103 90-91 3.6561718805476744 1103 92-93 3.5649298946006525 1103 94-95 3.437623048392604 1103 96-97 3.432480350936544 1103 98-99 3.120087042514939 1103 100-101 2.1453034054667066 1104 1 0.5933988673335051 1104 2 0.5056093031084608 1104 3 0.5028841081043396 1104 4 0.4498734065826753 1104 5 0.4591813154524047 1104 6 0.4320480662706885 1104 7 0.5004951938973008 1104 8 0.5607994025464151 1104 9 0.7064083244557793 1104 10-11 0.6971080784715156 1104 12-13 0.8098252038260867 1104 14-15 0.9270549785150735 1104 16-17 0.9046893853001805 1104 18-19 0.78591337854904 1104 20-21 0.7845338735280905 1104 22-23 0.8699162980171167 1104 24-25 0.9787277306050512 1104 26-27 0.9885252585768427 1104 28-29 1.0061341646038997 1104 30-31 0.8447915695912585 1104 32-33 1.019404891977814 1104 34-35 0.9198972371241041 1104 36-37 1.1671677593517202 1104 38-39 1.16772721044984 1104 40-41 1.1348998667954149 1104 42-43 1.1020333173925891 1104 44-45 1.1042625354204105 1104 46-47 1.1077420936060847 1104 48-49 1.1435731376320248 1104 50-51 1.196173913560493 1104 52-53 1.3028449014126053 1104 54-55 1.5719066835427427 1104 56-57 1.9493599884476396 1104 58-59 1.905447371081479 1104 60-61 1.8905213921418316 1104 62-63 2.1184346990722425 1104 64-65 2.3260714248403502 1104 66-67 2.3169204310079117 1104 68-69 2.5846854515909925 1104 70-71 2.599287624252465 1104 72-73 2.8666867555285336 1104 74-75 2.6379602548249927 1104 76-77 2.6988037575961457 1104 78-79 3.0206958944943914 1104 80-81 3.2618505613816 1104 82-83 3.1799679449654477 1104 84-85 3.311459446948003 1104 86-87 3.424530047445675 1104 88-89 3.545477630663129 1104 90-91 3.498504729710188 1104 92-93 3.9011637580600045 1104 94-95 3.770052235612285 1104 96-97 3.36618955077898 1104 98-99 3.3338805955081448 1104 100-101 2.355955984179901 1105 1 0.5586020604655637 1105 2 0.41070331361988366 1105 3 0.3034883817494709 1105 4 0.33429179455898606 1105 5 0.4380508831764729 1105 6 0.4149402145749974 1105 7 0.4439074417789399 1105 8 0.38716857730224064 1105 9 0.418338622497032 1105 10-11 0.5339548469672621 1105 12-13 0.5984818833886365 1105 14-15 0.6909434005558879 1105 16-17 0.6116280349362313 1105 18-19 0.6923370630300809 1105 20-21 0.6769501033065453 1105 22-23 0.5828460135407525 1105 24-25 0.7763154767404998 1105 26-27 0.7572058806469144 1105 28-29 0.7675228191622523 1105 30-31 0.7140329532528114 1105 32-33 0.7744757051256315 1105 34-35 0.8039478938569715 1105 36-37 0.8780647005087374 1105 38-39 0.9304949795487758 1105 40-41 1.1304251415876294 1105 42-43 1.2370656876410067 1105 44-45 1.044558395629231 1105 46-47 1.202716668969238 1105 48-49 1.0880203197098481 1105 50-51 0.9768082214006668 1105 52-53 1.2570306793890964 1105 54-55 1.6148041294395838 1105 56-57 1.7810104931058817 1105 58-59 1.6651922490328843 1105 60-61 1.81064321501405 1105 62-63 1.9048748202315586 1105 64-65 1.9514127361087183 1105 66-67 2.351589693168581 1105 68-69 2.601056589828243 1105 70-71 2.517913665890241 1105 72-73 2.6263510817906166 1105 74-75 2.633770533568647 1105 76-77 2.8520504366746238 1105 78-79 3.2167328710375855 1105 80-81 3.3165076836792977 1105 82-83 3.6247153058234183 1105 84-85 3.479945189407772 1105 86-87 3.439109413703491 1105 88-89 3.350015122008312 1105 90-91 3.3207136082359554 1105 92-93 3.671821731018351 1105 94-95 3.487350670102982 1105 96-97 3.2256883680669723 1105 98-99 2.8724965641054965 1105 100-101 2.126893739643248 1106 1 0.2648304993260808 1106 2 0.13818783479311136 1106 3 0.16937922400854788 1106 4 0.22841435975083613 1106 5 0.17825214558589408 1106 6 -0.20779821961550482 1106 7 0.1722461230280956 1106 8 -0.1286891229433138 1106 9 0.07855163525670861 1106 10-11 0.18296284624416614 1106 12-13 0.308535588515106 1106 14-15 0.35616612995134744 1106 16-17 -0.09467760975125827 1106 18-19 0.3127114924601955 1106 20-21 0.4035156868319518 1106 22-23 0.2760842895534594 1106 24-25 0.4727052569486929 1106 26-27 0.33106700054567995 1106 28-29 0.2930915059852879 1106 30-31 0.2791438283526091 1106 32-33 0.41382205423155227 1106 34-35 0.46868403823563654 1106 36-37 0.45363790669726 1106 38-39 0.4599469311614328 1106 40-41 0.6685456128068097 1106 42-43 0.3050241396076814 1106 44-45 0.7088362010253562 1106 46-47 0.8380453021624632 1106 48-49 0.642436116398656 1106 50-51 0.5873428623370742 1106 52-53 0.8626399651787011 1106 54-55 0.8729898301671994 1106 56-57 1.15168508372669 1106 58-59 1.2082181148679894 1106 60-61 1.5791737433278747 1106 62-63 1.4298123023424019 1106 64-65 1.5467012975946872 1106 66-67 1.7190683391666823 1106 68-69 1.9091455008118103 1106 70-71 1.880744144491043 1106 72-73 1.8503150172540757 1106 74-75 2.1796793779668953 1106 76-77 2.3802546668007167 1106 78-79 2.7343297737657757 1106 80-81 2.389348556820707 1106 82-83 2.3921924453663586 1106 84-85 2.4614026094526658 1106 86-87 2.119496873970739 1106 88-89 2.249914189512566 1106 90-91 2.348006432990026 1106 92-93 2.572559140819351 1106 94-95 2.532115361806932 1106 96-97 2.388668362342443 1106 98-99 2.414727021615928 1106 100-101 1.74292392900466 1107 1 0.2644953202608207 1107 2 0.14255331479838063 1107 3 0.2054658960681799 1107 4 0.18963862558639732 1107 5 0.21784048256679256 1107 6 0.2307619004511281 1107 7 0.2869272169221304 1107 8 0.2461891695351781 1107 9 0.42482348669514636 1107 10-11 0.28107342711669503 1107 12-13 0.28066696992944173 1107 14-15 0.43965059173132204 1107 16-17 0.38364742510444216 1107 18-19 0.4255769024405254 1107 20-21 0.47036703120623 1107 22-23 0.4411595088778384 1107 24-25 0.5049937555095241 1107 26-27 0.5515404631847716 1107 28-29 0.6080198297137116 1107 30-31 0.6459379508565348 1107 32-33 0.6834597640189486 1107 34-35 0.5053023772441207 1107 36-37 0.5713637789257788 1107 38-39 0.7818215718917045 1107 40-41 0.7973392921678482 1107 42-43 0.8253695193205175 1107 44-45 0.6550085020866021 1107 46-47 0.6889581347025313 1107 48-49 0.5695163796088067 1107 50-51 0.9110144265687623 1107 52-53 0.7931099056679543 1107 54-55 1.118333328613062 1107 56-57 1.2749223888326568 1107 58-59 1.3908857932382261 1107 60-61 1.6239704201742846 1107 62-63 1.6351244254357873 1107 64-65 1.5507684922702794 1107 66-67 1.6495406834970687 1107 68-69 1.6843494615058674 1107 70-71 1.9098150494431643 1107 72-73 1.8806191972202555 1107 74-75 1.7987393820220667 1107 76-77 1.9281104927709798 1107 78-79 2.169661430681206 1107 80-81 2.180756078962176 1107 82-83 2.403531468487131 1107 84-85 2.231402201844819 1107 86-87 2.3856725299580788 1107 88-89 2.5897633955133905 1107 90-91 2.5099955906213864 1107 92-93 2.3673128098125176 1107 94-95 2.3732684425906267 1107 96-97 2.2354101647216176 1107 98-99 1.9940441635115338 1107 100-101 1.4478175224545078 1108 1 0.3866951499557487 1108 2 0.3108327004205478 1108 3 0.16213620342465873 1108 4 0.2584178063245588 1108 5 0.16907595598595293 1108 6 0.24855192557036787 1108 7 0.2287078645993219 1108 8 0.338085968927075 1108 9 0.37695648916746194 1108 10-11 0.29125014528618465 1108 12-13 0.2679481667567103 1108 14-15 0.31344478460257363 1108 16-17 0.4148115775625172 1108 18-19 0.43487787476366435 1108 20-21 0.4552010446935384 1108 22-23 0.3567928441914674 1108 24-25 0.40182757401428404 1108 26-27 0.3763998244036486 1108 28-29 0.5110722206110694 1108 30-31 0.448647294328957 1108 32-33 0.556204250049511 1108 34-35 0.5275060151919959 1108 36-37 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-0.20441078435622728 2315 92-93 0.031241606016600088 2315 94-95 0.009751094035092223 2315 96-97 -0.00907314855770025 2315 98-99 -0.11240694605669255 2315 100-101 -0.05678395766793276 2316 1 -1.0985627270429 2316 2 -0.9256257076553105 2316 3 -0.7977728884024806 2316 4 -0.7848164071236212 2316 5 -0.6708788875689677 2316 6 -0.5741899997120825 2316 7 -0.7316939299654237 2316 8 -0.6963093593793133 2316 9 -0.8467940150954547 2316 10-11 -1.041748500977178 2316 12-13 -0.9175541482020506 2316 14-15 -1.1988724117859775 2316 16-17 -1.143237627867947 2316 18-19 -0.9802667018654319 2316 20-21 -1.0307965242144164 2316 22-23 -0.9969374789247993 2316 24-25 -0.9656630808445286 2316 26-27 -1.0831092587068483 2316 28-29 -1.191827603408484 2316 30-31 -1.0667168459472833 2316 32-33 -1.2961540527357158 2316 34-35 -1.0463853106354648 2316 36-37 -1.3419673178250449 2316 38-39 -1.393824973520239 2316 40-41 -1.1939814364255241 2316 42-43 -1.1236526414844406 2316 44-45 -1.2371631928967943 2316 46-47 -0.9173476966538132 2316 48-49 -0.7178394739802414 2316 50-51 -0.7080993009888701 2316 52-53 -0.8275377447222496 2316 54-55 -0.7885651971063048 2316 56-57 -0.8575748886606647 2316 58-59 -0.6497491831844684 2316 60-61 -0.6944728651524024 2316 62-63 -0.4513359963730217 2316 64-65 -0.40165354019744015 2316 66-67 -0.20861738430865984 2316 68-69 -0.24339214931279685 2316 70-71 -0.29379303740013896 2316 72-73 -0.2920556223221489 2316 74-75 -0.3054944154021335 2316 76-77 -0.3434881045722804 2316 78-79 -0.4255655957557316 2316 80-81 -0.4966102386378637 2316 82-83 -0.6140880089513452 2316 84-85 -0.3786001146156259 2316 86-87 -0.2735367316235582 2316 88-89 -0.3722647588001493 2316 90-91 -0.2975017534368938 2316 92-93 -0.26946273049522773 2316 94-95 -0.4163503532227022 2316 96-97 -0.22316672978357843 2316 98-99 -0.26796934651181736 2316 100-101 0.31825043429854194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9259.0 3 3472.0 4 3905.0 5 4480.0 6 5429.0 7 6963.0 8 8788.0 9 11602.0 10 14797.0 11 18547.0 12 22756.0 13 27043.0 14 30958.0 15 35359.0 16 40633.0 17 46147.0 18 47527.0 19 48556.0 20 52852.0 21 58895.0 22 65702.0 23 72628.0 24 80869.0 25 89107.0 26 98112.0 27 107917.0 28 120252.0 29 132385.0 30 147292.0 31 163389.0 32 182768.0 33 205000.0 34 229669.0 35 251248.0 36 250708.0 37 178719.0 38 43953.0 39 524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.05526553249781 18.710965142076134 14.527236805938509 23.706532519487542 2 15.349043034975601 19.865268044813302 40.40902902224898 24.37665989796212 3 19.276402188008735 23.754230551290583 29.33257643615925 27.63679082454143 4 12.832440448948235 16.36685018020529 36.92180636561944 33.87890300522704 5 14.117443341671281 37.002144778552896 34.17508319374842 14.705328686027395 6 32.693965953207005 35.756298444130614 17.477070306754396 14.072665295907989 7 29.284509305770428 31.879175649737867 20.450823620075138 18.38549142441656 8 26.311771247718525 35.31079489278608 19.714656157138293 18.662777702357104 9 26.3301639582542 16.83561392129949 19.92256650819791 36.911655612248396 10-11 25.00227539768038 26.482063781410986 27.535002986727275 20.980657834181358 12-13 25.842123529789834 23.572500374916984 27.85272403967693 22.732652055616256 14-15 22.63741940260623 25.278167162006827 25.81087563719951 26.273537798187434 16-17 22.584038996006726 28.39645665964989 26.517195455949977 22.502308888393415 18-19 22.492447109198313 27.08121816971536 28.56795682221246 21.858377898873872 20-21 22.944341158905456 26.414112527742695 28.683301627805612 21.958244685546237 22-23 22.69179647630776 26.22722921653165 28.498664411119712 22.58230989604088 24-25 22.553498931692605 26.40859308244179 28.61757680628797 22.42033117957763 26-27 22.285727703859767 26.764263923266423 28.386578926033675 22.563429446840136 28-29 22.238637725030692 26.971358584909005 28.523257749083786 22.266745940976516 30-31 22.367423570472305 26.657167876125627 28.422825411989898 22.55258314141217 32-33 22.191058271218235 26.64394411877973 28.37975619449939 22.785241415502647 34-35 22.772082590001496 26.742481515181794 28.190005472476255 22.295430422340452 36-37 22.759983210275532 26.46116107903288 28.168893384735437 22.609962325956147 38-39 22.393611661572564 26.773093276540795 28.146752604914603 22.68654245697204 40-41 22.715784513226946 26.54164721002463 28.214749919674414 22.527818357074008 42-43 22.59239336906676 26.51089798700018 28.074810915517272 22.821897728415784 44-45 22.551468860788734 26.804985097793534 28.034226494688824 22.60931954672891 46-47 22.676599356691003 26.93464410383773 27.62167096847498 22.76708557099629 48-49 22.606608813316267 26.889589258806147 27.680410136257954 22.82339179161963 50-51 22.746478185389616 26.99081978011546 27.56975642506579 22.692945609429135 52-53 22.865295968391013 26.922059992025098 27.503556062235756 22.709087977348126 54-55 22.527885563592964 26.923656228125715 27.71145177997243 22.837006428308893 56-57 22.61445819880649 27.139443779402132 27.5624986505905 22.683599371200877 58-59 22.639174607678545 26.744615207470396 27.683349217070546 22.932860967780517 60-61 22.561656104849263 26.834201542539965 27.818752667727786 22.785389684882983 62-63 22.474845746683382 26.923589023617247 27.826977875635333 22.774587354064032 64-65 22.33608688731082 26.79856469521021 27.730577231937158 23.134771185541812 66-67 22.323092599760493 26.902371284891906 27.633771477791296 23.140764637556302 68-69 22.52342511468385 26.653277459868363 27.759591942761226 23.063705482686558 70-71 22.47683720687937 26.77270680294164 27.53500380077496 23.21545218940403 72-73 22.55833555954894 26.740976287076773 27.4645663300583 23.23612182331599 74-75 22.52490008094968 26.91420978785152 27.326823544088104 23.2340665871107 76-77 22.56078430633402 26.715880006586605 27.423905777963896 23.299429909115474 78-79 22.649726355810614 26.762057299837235 27.32212870658547 23.266087637766685 80-81 22.64431939807756 26.97802252411107 27.14172176714986 23.23593631066151 82-83 22.725319979895815 26.897503787527626 27.0953411653744 23.28183506720216 84-85 22.744187584701827 26.70264275701163 27.141717157195455 23.41145250109109 86-87 22.839054971106528 26.64818567214037 27.081099456399006 23.431659900354095 88-89 22.870923645516466 26.595930084605257 26.951206701519144 23.581939568359132 90-91 22.824666171375785 26.691585453529633 26.98457828723615 23.49917008785843 92-93 22.926877749741895 26.377867549725615 27.027755525187313 23.667499175345178 94-95 22.800452086616232 26.292332778935982 26.998225414362746 23.908989720085035 96-97 22.966217733076736 26.164042698674105 26.852163809634792 24.017575758614367 98-99 22.8746253249811 26.17771162584111 26.510318171318588 24.4373448778592 100-101 23.774993982727555 25.332073666168416 26.747209195044203 24.145723156059827 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 286.0 1 225.0 2 213.0 3 260.5 4 228.5 5 258.5 6 441.0 7 902.0 8 1447.5 9 1695.0 10 1557.0 11 1389.5 12 1466.5 13 1651.5 14 1855.0 15 2038.0 16 2291.5 17 2509.5 18 2771.0 19 3119.5 20 3574.5 21 4213.0 22 5077.5 23 6121.5 24 7538.5 25 9499.0 26 12190.0 27 15749.0 28 19465.0 29 23858.0 30 29236.0 31 35202.0 32 41931.5 33 47218.5 34 51744.0 35 57907.0 36 64422.0 37 70235.5 38 75357.0 39 80195.5 40 85582.5 41 91922.5 42 98043.5 43 103241.5 44 107969.5 45 112803.5 46 115240.5 47 116849.0 48 119278.5 49 118697.5 50 116182.0 51 113021.5 52 108941.0 53 104863.5 54 101067.5 55 94214.5 56 84618.0 57 75873.5 58 67971.5 59 59068.0 60 49354.5 61 41222.0 62 33884.5 63 26525.5 64 20346.0 65 15550.5 66 11620.0 67 8874.5 68 6733.5 69 4960.0 70 3830.0 71 3004.0 72 2437.0 73 1987.0 74 1549.5 75 1214.0 76 901.5 77 573.5 78 383.5 79 248.5 80 128.5 81 81.0 82 49.5 83 31.0 84 21.5 85 15.0 86 10.5 87 8.0 88 4.0 89 2.5 90 2.5 91 3.0 92 3.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3890398566244376 2 0.029538655545694105 3 0.035535482367615764 4 0.043142885535996385 5 0.062538336857183 6 0.05403997656097402 7 0.039373451533645625 8 0.04242326631736578 9 0.03858529715133592 10-11 0.03897937434249077 12-13 0.030755154700998218 14-15 0.029281648681897466 16-17 0.02331908944181536 18-19 0.026865784162209028 20-21 0.034284715630472105 22-23 0.03281120961137135 24-25 0.041549442980457194 26-27 0.03603236230428927 28-29 0.031303436010431056 30-31 0.0341476453031139 32-33 0.028904705281662387 34-35 0.030275408555244483 36-37 0.03253706895665494 38-39 0.015626017318835863 40-41 0.013621363781222052 42-43 0.013724166526740708 44-45 0.012113590180281748 46-47 0.02187985100455416 48-49 0.018692965893475796 50-51 0.012353463253158614 52-53 0.010468746251983237 54-55 0.008823902323684725 56-57 0.009149444351160472 58-59 0.004489053220981355 60-61 0.007898677614016811 62-63 0.012456265998677272 64-65 0.007658804541139946 66-67 0.003718032629591428 68-69 0.006442305385835838 70-71 0.013158751426388095 72-73 0.01125690063429294 74-75 0.01192511848016421 76-77 0.005705552376285463 78-79 0.009543521542315324 80-81 0.012302061880399286 82-83 0.014032574763296678 84-85 0.008686831996326515 86-87 0.0026386038016455293 88-89 0.01656837581942355 90-91 0.025101003697472077 92-93 0.011051295143255627 94-95 0.021502907604319086 96-97 0.019018507920951545 98-99 0.01192511848016421 100-101 0.12622463770599102 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2918210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.89024996138997 #Duplication Level Percentage of deduplicated Percentage of total 1 74.21429116891014 39.994267017999434 2 13.604926650909995 14.663457958478315 3 5.086865310432524 8.223973292973968 4 2.3999481875344895 5.173352308824739 5 1.293657887343553 3.485777345673387 6 0.8112050254757712 2.6229624955695012 7 0.5289642823641847 1.9954212178077226 8 0.35400859136882246 1.5262089181876322 9 0.2661002324769822 1.2906187238671696 >10 1.3192524322423618 12.491234589299136 >50 0.07923218790195925 2.903008235733691 >100 0.03829170908192109 3.6979071590389267 >500 0.0022985886758695143 0.8636597525810686 >1k 8.300459106564586E-4 0.669525556357209 >5k 1.276993708702244E-4 0.3986254276080926 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6427 0.2202377484828028 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5158 0.1767521871284109 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3613 0.12380877318630257 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.426758183955233E-5 6 0.0 0.0 0.0 0.0 3.426758183955233E-5 7 6.853516367910466E-5 0.0 0.0 0.0 3.426758183955233E-5 8 6.853516367910466E-5 0.0 0.0 0.0 3.426758183955233E-5 9 6.853516367910466E-5 0.0 0.0 3.426758183955233E-5 3.426758183955233E-5 10-11 6.853516367910466E-5 0.0 0.0 6.853516367910466E-5 3.426758183955233E-5 12-13 1.0280274551865699E-4 0.0 0.0 6.853516367910466E-5 6.853516367910466E-5 14-15 1.0280274551865699E-4 0.0 0.0 6.853516367910466E-5 6.853516367910466E-5 16-17 1.0280274551865699E-4 0.0 0.0 6.853516367910466E-5 6.853516367910466E-5 18-19 1.0280274551865699E-4 0.0 0.0 6.853516367910466E-5 6.853516367910466E-5 20-21 1.0280274551865699E-4 0.0 0.0 1.1993653643843316E-4 6.853516367910466E-5 22-23 1.0280274551865699E-4 0.0 0.0 2.0560549103731398E-4 6.853516367910466E-5 24-25 1.0280274551865699E-4 0.0 0.0 5.140137275932849E-4 6.853516367910466E-5 26-27 1.0280274551865699E-4 0.0 0.0 8.052881732294797E-4 6.853516367910466E-5 28-29 1.3707032735820932E-4 0.0 0.0 0.002809941710843291 6.853516367910466E-5 30-31 1.3707032735820932E-4 0.0 0.0 0.011085562725095179 6.853516367910466E-5 32-33 1.5420411827798549E-4 1.7133790919776165E-5 0.0 0.027054255862326564 1.0280274551865699E-4 34-35 2.0560549103731398E-4 3.426758183955233E-5 0.0 0.052120991977959094 1.0280274551865699E-4 36-37 2.398730728768663E-4 3.426758183955233E-5 0.0 0.09708005935145175 1.0280274551865699E-4 38-39 2.7414065471641864E-4 3.426758183955233E-5 0.0 0.16242833791947803 1.0280274551865699E-4 40-41 2.9127444563619483E-4 3.426758183955233E-5 0.0 0.23293388755435696 1.0280274551865699E-4 42-43 3.426758183955233E-4 3.426758183955233E-5 0.0 0.3075172794281426 1.0280274551865699E-4 44-45 3.7694340023507563E-4 3.426758183955233E-5 0.0 0.3977095548298443 1.3707032735820932E-4 46-47 4.1121098207462796E-4 3.426758183955233E-5 0.0 0.5084109779625181 1.7133790919776165E-4 48-49 4.1121098207462796E-4 3.426758183955233E-5 0.0 0.6249378900079159 2.0560549103731398E-4 50-51 4.1121098207462796E-4 3.426758183955233E-5 0.0 0.7497232892766457 3.0840823655597097E-4 52-53 4.2834477299440415E-4 3.426758183955233E-5 0.0 0.875999328355396 3.426758183955233E-4 54-55 4.797461457537326E-4 3.426758183955233E-5 0.0 1.0183297295259766 3.598096093152995E-4 56-57 4.797461457537326E-4 3.426758183955233E-5 0.0 1.1823686437919134 3.9407719115485177E-4 58-59 4.797461457537326E-4 3.426758183955233E-5 0.0 1.358161338628817 4.1121098207462796E-4 60-61 4.797461457537326E-4 3.426758183955233E-5 0.0 1.5534865551142651 4.1121098207462796E-4 62-63 4.797461457537326E-4 3.426758183955233E-5 0.0 1.7487089688541948 4.454785639141803E-4 64-65 4.797461457537326E-4 3.426758183955233E-5 0.0 1.9579468235665014 4.454785639141803E-4 66-67 4.797461457537326E-4 3.426758183955233E-5 0.0 2.199259134880629 4.6261235483395643E-4 68-69 4.797461457537326E-4 3.426758183955233E-5 0.0 2.4620058186353964 4.797461457537326E-4 70-71 4.797461457537326E-4 3.426758183955233E-5 0.0 2.735306917596746 4.797461457537326E-4 72-73 4.797461457537326E-4 3.426758183955233E-5 0.0 3.0157014059988825 4.797461457537326E-4 74-75 4.797461457537326E-4 3.426758183955233E-5 0.0 3.30452571953355 4.968799366735087E-4 76-77 4.797461457537326E-4 3.426758183955233E-5 0.0 3.6187937125840843 5.140137275932849E-4 78-79 4.797461457537326E-4 3.426758183955233E-5 0.0 3.9605100386881 5.140137275932849E-4 80-81 4.797461457537326E-4 3.426758183955233E-5 0.0 4.328972212417886 5.140137275932849E-4 82-83 4.797461457537326E-4 6.853516367910466E-5 0.0 4.704082297024546 5.140137275932849E-4 84-85 5.311475185130611E-4 6.853516367910466E-5 0.0 5.0785755651580935 5.140137275932849E-4 86-87 5.482813094328373E-4 6.853516367910466E-5 0.0 5.468026632764605 5.140137275932849E-4 88-89 5.482813094328373E-4 6.853516367910466E-5 0.0 5.884771829306321 5.140137275932849E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3875 0.0 54.913113 1 GTATCAA 7445 0.0 44.79457 1 ATCAACG 9370 0.0 34.896572 3 TATCAAC 9440 0.0 34.73671 2 TCAACGC 9575 0.0 34.19907 4 CAACGCA 9710 0.0 33.674652 5 AACGCAG 10120 0.0 32.25621 6 ACGCAGA 11795 0.0 27.63098 7 CGCAGAG 12010 0.0 26.978106 8 GTACATG 9290 0.0 24.753935 1 GCAGAGT 13035 0.0 24.090076 9 TACATGG 9290 0.0 23.787502 2 GGACCGA 700 0.0 23.079052 6 ACATGGG 9845 0.0 21.771822 3 GAGTACT 7350 0.0 21.65106 12-13 GTACTAG 590 0.0 21.024918 1 TGGTATC 1980 0.0 20.881691 2 GTGGTAT 2375 0.0 20.69124 1 AGAGTAC 12025 0.0 20.245071 10-11 CAGAGTA 12630 0.0 20.196749 10-11 >>END_MODULE