FastQCFastQC Report
Thu 26 May 2016
SRR938408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918210
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68610.23510987900116853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58050.19892331257860127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40610.139160649850422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41500.055.5310331
GTATCAA75150.040.0236631
ATCAACG93350.031.648053
TCAACGC95000.031.1478374
TATCAAC96650.030.7180522
CAACGCA96950.030.5213475
AACGCAG101100.029.3624526
ACGCAGA117500.025.062097
CGCAGAG119150.024.6353028
TCGCGTA1601.8623159E-623.7483529
GTACATG92550.023.616931
TACATGG89800.023.4338112
TGGTATC20000.022.5636392
TAAGGTG13800.022.0274565
GTGGTAT23300.021.616831
ACATGGG93350.021.573593
GAGTACT75650.021.50379412-13
GCAGAGT136250.021.2297179
GTAAGGT12400.020.301014
GTACTTT80900.020.02024314-15
Produced by FastQC (version 0.11.5)