##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938407_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 481772 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.053404930132928 31.0 31.0 34.0 28.0 34.0 2 31.0680218028445 31.0 31.0 34.0 28.0 34.0 3 31.092381873583356 31.0 31.0 34.0 27.0 34.0 4 34.71893758873492 37.0 35.0 37.0 32.0 37.0 5 34.69585613111597 37.0 35.0 37.0 32.0 37.0 6 34.74982979500677 37.0 35.0 37.0 32.0 37.0 7 34.68121642602725 37.0 35.0 37.0 32.0 37.0 8 34.66120488529844 37.0 35.0 37.0 32.0 37.0 9 36.15359547669852 39.0 37.0 39.0 32.0 39.0 10-11 36.03175464742658 39.0 35.0 39.0 31.5 39.0 12-13 35.93155476034306 39.0 35.0 39.0 31.0 39.0 14-15 36.8759579220046 39.0 36.0 41.0 31.0 41.0 16-17 36.734852793437554 39.0 36.0 41.0 31.0 41.0 18-19 36.87443541758343 39.0 36.0 41.0 31.0 41.0 20-21 36.84819375140108 39.0 36.0 41.0 31.0 41.0 22-23 36.777863802794684 39.0 36.0 41.0 30.5 41.0 24-25 36.621069717625765 39.0 36.0 41.0 30.0 41.0 26-27 36.38980056956403 39.0 36.0 41.0 30.0 41.0 28-29 36.194088697558186 39.0 35.0 41.0 30.0 41.0 30-31 35.938557035278095 39.0 35.0 40.0 29.0 41.0 32-33 35.60598270551215 38.0 35.0 40.0 27.5 41.0 34-35 35.325108142440826 38.0 35.0 40.0 27.0 41.0 36-37 35.002325788962416 38.0 34.5 40.0 25.5 41.0 38-39 34.80815095107229 38.0 34.0 40.0 25.0 41.0 40-41 34.71056537117143 38.0 34.0 40.0 24.0 41.0 42-43 34.38087622360785 38.0 33.5 40.0 23.5 41.0 44-45 34.0065196815091 38.0 33.0 40.0 22.5 41.0 46-47 33.56997604676071 38.0 33.0 40.0 19.0 41.0 48-49 33.136747258039065 38.0 33.0 40.0 15.5 41.0 50-51 32.35602318108981 36.5 31.5 39.5 12.5 40.5 52-53 32.347069775744544 37.0 31.5 39.0 9.0 40.0 54-55 32.430572968126 37.0 32.0 40.0 7.5 41.0 56-57 32.05741927716845 36.5 31.0 40.0 4.0 41.0 58-59 31.67927359829961 36.0 31.0 39.0 2.0 41.0 60-61 31.162437833664058 35.5 30.0 39.0 2.0 40.5 62-63 30.63796256320417 35.0 29.5 39.0 2.0 40.0 64-65 30.14366754398346 35.0 29.0 38.0 2.0 40.0 66-67 29.59688400322144 34.5 28.0 37.5 2.0 40.0 68-69 29.0599152711241 34.0 27.5 37.0 2.0 39.0 70-71 28.423070456564517 34.0 26.0 36.0 2.0 39.0 72-73 27.834779729830707 34.0 26.0 36.0 2.0 38.5 74-75 27.230025821342878 33.0 25.0 35.0 2.0 37.5 76-77 26.5792553323979 33.0 23.5 35.0 2.0 37.0 78-79 25.8379316357115 32.0 20.5 35.0 2.0 36.0 80-81 25.189809702514882 31.5 19.0 35.0 2.0 36.0 82-83 24.51874434379748 31.0 14.5 34.5 2.0 35.0 84-85 23.866466917961194 31.0 7.0 34.0 2.0 35.0 86-87 23.213502445140023 30.5 2.0 34.0 2.0 35.0 88-89 22.421472190164643 29.5 2.0 34.0 2.0 35.0 90-91 21.57998804413706 29.0 2.0 34.0 2.0 35.0 92-93 20.702714146940878 28.5 2.0 34.0 2.0 35.0 94-95 19.74117217272901 27.0 2.0 33.5 2.0 35.0 96-97 18.553545037901745 25.0 2.0 33.0 2.0 35.0 98-99 17.114458706608104 20.5 2.0 32.5 2.0 34.0 100-101 13.60797742500602 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22168845706688245 1101 2 0.3844564698965307 1101 3 0.25274175240443597 1101 4 0.2706587295931939 1101 5 0.1663869814739698 1101 6 0.41166569071708636 1101 7 0.40991011932232624 1101 8 0.5399048422578829 1101 9 0.3806824684822061 1101 10-11 0.5699504255271606 1101 12-13 0.6151343783490546 1101 14-15 0.6813864618384642 1101 16-17 0.810208123835146 1101 18-19 0.7797789198556515 1101 20-21 0.8563867042228779 1101 22-23 0.7884360892773046 1101 24-25 0.6160590905244021 1101 26-27 0.8224459647091678 1101 28-29 0.7651333437942682 1101 30-31 0.5256965290382354 1101 32-33 0.5242152193163818 1101 34-35 0.1431873324586661 1101 36-37 0.16100538043236412 1101 38-39 0.31609154762836056 1101 40-41 0.3151020945432137 1101 42-43 0.539069244928676 1101 44-45 0.6055370690681201 1101 46-47 0.298592308696378 1101 48-49 0.11035723600729597 1101 50-51 0.1029507068905744 1101 52-53 -0.04324208828072784 1101 54-55 0.19594916508453508 1101 56-57 0.24760999848120235 1101 58-59 0.22897206165257344 1101 60-61 0.10960934158905289 1101 62-63 0.07249839149647741 1101 64-65 0.22627345867303816 1101 66-67 0.20714020906235575 1101 68-69 0.04854920240230598 1101 70-71 0.13951225711722515 1101 72-73 0.2035410655162302 1101 74-75 0.13915766778136884 1101 76-77 -0.10338991499715178 1101 78-79 0.4971102230221902 1101 80-81 0.26222979993976736 1101 82-83 0.29790348192552685 1101 84-85 0.42511288150422644 1101 86-87 0.5433710086508121 1101 88-89 0.43199520955743154 1101 90-91 0.041183580921643426 1101 92-93 -0.23712499253640473 1101 94-95 -0.2079801296874031 1101 96-97 -0.34304763340827193 1101 98-99 0.10540895466484201 1101 100-101 0.4321148921644209 1102 1 0.49414501069148997 1102 2 0.4151985769531379 1102 3 0.12334340877434613 1102 4 0.22147135830262243 1102 5 0.4310654116264203 1102 6 0.6019852604757432 1102 7 0.37088616270203545 1102 8 0.43330751200616646 1102 9 0.40595766589839144 1102 10-11 0.5995739643511087 1102 12-13 0.7266282225199063 1102 14-15 0.9137745679765104 1102 16-17 0.6676388244588694 1102 18-19 0.7863273590455435 1102 20-21 0.6991278630891955 1102 22-23 0.6641638994021974 1102 24-25 0.2368422364420013 1102 26-27 0.0168620128298258 1102 28-29 0.2581700713525308 1102 30-31 0.2468026522733453 1102 32-33 0.3205024617123371 1102 34-35 0.4101547532329235 1102 36-37 0.4574407775611249 1102 38-39 0.3670864066591406 1102 40-41 0.7081195619862157 1102 42-43 0.8613576429315444 1102 44-45 0.6456499629745167 1102 46-47 0.217010905078439 1102 48-49 0.769134663152208 1102 50-51 0.588838968591098 1102 52-53 0.4778847766169747 1102 54-55 0.3112283626784986 1102 56-57 0.4122987994649421 1102 58-59 0.5928474931532328 1102 60-61 0.618120830988584 1102 62-63 0.5621349679369096 1102 64-65 0.6947165005860114 1102 66-67 0.24525301407591016 1102 68-69 0.15639103240956587 1102 70-71 0.4234211715874956 1102 72-73 0.6885996607130522 1102 74-75 0.3492855253157785 1102 76-77 0.4653983274432534 1102 78-79 0.7252833270119972 1102 80-81 0.6690182497967996 1102 82-83 0.9001746939793946 1102 84-85 0.4324983949344521 1102 86-87 0.33909526306212 1102 88-89 0.7400014913181003 1102 90-91 0.5437985119556146 1102 92-93 0.8540938764503672 1102 94-95 1.048809060943288 1102 96-97 1.380169694769279 1102 98-99 1.3936958414894427 1102 100-101 1.2473807558324346 1103 1 0.19894402923807064 1103 2 0.21997594579199387 1103 3 0.3079721162867948 1103 4 0.1802791810636819 1103 5 0.1456933974819492 1103 6 0.3403846254176486 1103 7 0.26128090903962686 1103 8 0.4566747632149415 1103 9 0.3729490781809872 1103 10-11 0.5409537723903668 1103 12-13 0.35454191235176324 1103 14-15 0.6883650276719777 1103 16-17 0.5230187768295238 1103 18-19 0.5005223507464862 1103 20-21 0.3920628418003176 1103 22-23 0.6316604715318093 1103 24-25 0.7236286981581017 1103 26-27 0.7131815625159064 1103 28-29 0.8355100439295668 1103 30-31 0.9092043265174112 1103 32-33 0.9803855532906027 1103 34-35 0.8621790009055772 1103 36-37 0.6201166814364214 1103 38-39 0.3169745498358765 1103 40-41 0.8971287270291484 1103 42-43 0.9452502603812079 1103 44-45 1.0768038923964696 1103 46-47 0.8597402115661339 1103 48-49 1.020148591130848 1103 50-51 1.2386838123523702 1103 52-53 1.1570000252277808 1103 54-55 0.9011973314307582 1103 56-57 0.9428104542242792 1103 58-59 0.9304674686169001 1103 60-61 0.8448582200338315 1103 62-63 1.2096272101249745 1103 64-65 1.4009227501349777 1103 66-67 1.196230859208331 1103 68-69 0.9637952324528634 1103 70-71 1.413029311877601 1103 72-73 1.6247402394819055 1103 74-75 1.6007613137259682 1103 76-77 1.507369894160437 1103 78-79 1.3627301048138278 1103 80-81 1.3984257694619515 1103 82-83 1.385149796815508 1103 84-85 1.5851071847157918 1103 86-87 1.9049248076965988 1103 88-89 1.5366024872046573 1103 90-91 1.9856185307186927 1103 92-93 2.035886472250322 1103 94-95 2.116601512411865 1103 96-97 1.9944657184396242 1103 98-99 2.5758853485977617 1103 100-101 2.651797296209139 1104 1 0.3980341863927457 1104 2 0.34203274204233836 1104 3 0.3523200622476459 1104 4 0.04195549757870509 1104 5 0.04399778127019971 1104 6 0.3931141539536256 1104 7 0.46249403283339774 1104 8 0.4386709032756002 1104 9 0.2605007737972045 1104 10-11 0.6728258429721166 1104 12-13 0.6272190559966475 1104 14-15 0.6515922126733571 1104 16-17 0.8215781923244165 1104 18-19 0.829313362602015 1104 20-21 0.7174695139811789 1104 22-23 0.8031244913829241 1104 24-25 0.6048942068429639 1104 26-27 0.9196469609167863 1104 28-29 0.9185125253191515 1104 30-31 0.7968800916125289 1104 32-33 0.7905592505610741 1104 34-35 0.6579606386227042 1104 36-37 0.7389614567328167 1104 38-39 0.2317181395794563 1104 40-41 0.8632922230313582 1104 42-43 0.9238827390136848 1104 44-45 0.8169127978279533 1104 46-47 0.41593679276271445 1104 48-49 0.46306284533542197 1104 50-51 0.4256427315693543 1104 52-53 0.6082338272680374 1104 54-55 0.48799082467586175 1104 56-57 0.5680379149356085 1104 58-59 0.24738916699020663 1104 60-61 0.41887944970022417 1104 62-63 0.6554708825815503 1104 64-65 0.48538649427613834 1104 66-67 0.6957276885438723 1104 68-69 0.8476662001948014 1104 70-71 0.8632981290819046 1104 72-73 1.0485133301904774 1104 74-75 0.6758715977072214 1104 76-77 0.5859885872952582 1104 78-79 0.8400943176619151 1104 80-81 0.9265757556764527 1104 82-83 1.1348947651088643 1104 84-85 1.3204049514651715 1104 86-87 1.2341362772306503 1104 88-89 1.157535765396002 1104 90-91 1.2096190821385981 1104 92-93 1.58565901153899 1104 94-95 2.055455937556612 1104 96-97 1.5155092806122177 1104 98-99 1.678249561445849 1104 100-101 1.630705484794749 1105 1 0.21908439367049937 1105 2 0.015327178809830144 1105 3 0.21627164625910922 1105 4 0.16465880890477536 1105 5 -0.034191993027356204 1105 6 -0.05456740600337895 1105 7 0.08366195340251181 1105 8 -0.004621998101136171 1105 9 -0.14303830189756184 1105 10-11 0.14692891887159476 1105 12-13 -0.09784363912592653 1105 14-15 0.11647654656966466 1105 16-17 0.1072696299619551 1105 18-19 0.0504032341409868 1105 20-21 0.19841787367206365 1105 22-23 0.2832411810804487 1105 24-25 0.30384246417445127 1105 26-27 0.182262754969706 1105 28-29 -0.0943463722702873 1105 30-31 0.29580531808266386 1105 32-33 0.018180930278560936 1105 34-35 0.08694881611387473 1105 36-37 0.24173795501829431 1105 38-39 0.21457934025502823 1105 40-41 0.596678015548882 1105 42-43 0.1897612384251275 1105 44-45 0.41902268062645476 1105 46-47 -0.043253800409914334 1105 48-49 -0.07784252341425457 1105 50-51 -0.0029104185406865213 1105 52-53 -0.027373163877875584 1105 54-55 0.03679066090084859 1105 56-57 0.30464163058123717 1105 58-59 0.20757776415485196 1105 60-61 0.08777883042595036 1105 62-63 0.09457344668411594 1105 64-65 0.14313509962953574 1105 66-67 0.06883403994363846 1105 68-69 -0.1673038596854397 1105 70-71 0.0012721809781091054 1105 72-73 0.03473959560836093 1105 74-75 -0.01836173050814338 1105 76-77 -0.051467270220481254 1105 78-79 0.2107888260809716 1105 80-81 0.3067896867885871 1105 82-83 0.39626305417176155 1105 84-85 0.27858474572280656 1105 86-87 0.0034310210762917848 1105 88-89 0.03892043196030315 1105 90-91 0.02052291549752283 1105 92-93 0.528945515454236 1105 94-95 0.7635205775074745 1105 96-97 1.1994171059871093 1105 98-99 0.691409139725188 1105 100-101 0.4203569510929217 1106 1 0.19677582805019966 1106 2 0.052759501023718514 1106 3 0.0818300571580508 1106 4 0.11444711729825485 1106 5 0.30581792553388 1106 6 0.05626636792719353 1106 7 0.18577804975191015 1106 8 -0.10825866726793265 1106 9 -0.05399667263043284 1106 10-11 0.051052909084191356 1106 12-13 0.17999288495117582 1106 14-15 0.06919124038531521 1106 16-17 0.1314840348277997 1106 18-19 -0.0934322980799891 1106 20-21 0.28794723384289256 1106 22-23 0.34171765728614645 1106 24-25 0.28660589009468396 1106 26-27 0.43239288485367666 1106 28-29 0.656587079102124 1106 30-31 0.20216098457309073 1106 32-33 0.1765172350816897 1106 34-35 -0.03455765759169083 1106 36-37 0.3433376304108364 1106 38-39 0.43210117543477367 1106 40-41 0.7996744866723517 1106 42-43 0.22382657797190575 1106 44-45 0.4999122362175328 1106 46-47 0.30676279883513047 1106 48-49 0.8228489068668665 1106 50-51 0.34027794805262346 1106 52-53 0.09396321879825109 1106 54-55 0.5244867266748443 1106 56-57 -0.10692128055746863 1106 58-59 0.0581374251203961 1106 60-61 0.6108558796129948 1106 62-63 0.3365310099969143 1106 64-65 0.8120375411530603 1106 66-67 0.31657264896788817 1106 68-69 0.21864498185119885 1106 70-71 0.28124816330503677 1106 72-73 0.7518228828326805 1106 74-75 0.9534892287642762 1106 76-77 0.87489765905854 1106 78-79 1.0519442974790394 1106 80-81 1.1430063704643558 1106 82-83 0.6198343919889417 1106 84-85 0.8363911042583041 1106 86-87 0.720846293201447 1106 88-89 0.6571562396057935 1106 90-91 0.9471583509920372 1106 92-93 0.7675483918369927 1106 94-95 0.6439424852320705 1106 96-97 0.4901293160638751 1106 98-99 0.4098634016325846 1106 100-101 0.37276835006089826 1107 1 -0.1838103925727559 1107 2 4.394877811364495E-4 1107 3 -0.06293577383352655 1107 4 -0.2421053978065686 1107 5 -0.029763230590617695 1107 6 -0.08000214113963722 1107 7 -0.16073217580548516 1107 8 -0.2380438300610166 1107 9 -0.2035353500277921 1107 10-11 0.20256812031516347 1107 12-13 -0.10718388378057142 1107 14-15 0.3123305200047568 1107 16-17 0.22176808027821693 1107 18-19 0.10318059688191994 1107 20-21 0.27546531429944565 1107 22-23 0.1749931857084306 1107 24-25 0.26982604403967514 1107 26-27 0.2506384795282699 1107 28-29 0.5171025149839963 1107 30-31 0.3056895802684636 1107 32-33 0.20049576318473328 1107 34-35 0.5229884451092417 1107 36-37 0.33584532568961833 1107 38-39 0.6577295082491759 1107 40-41 0.3764715953757971 1107 42-43 0.49867316505172 1107 44-45 0.14607720880618302 1107 46-47 0.43958666070241037 1107 48-49 0.8550153692457272 1107 50-51 0.5293551846100257 1107 52-53 0.6873490232258419 1107 54-55 0.6767468330096094 1107 56-57 0.38040564956821754 1107 58-59 0.5381986720511556 1107 60-61 0.7184656334034898 1107 62-63 0.8271390256988305 1107 64-65 0.8635752174744589 1107 66-67 0.7341789738761584 1107 68-69 0.9078069551532124 1107 70-71 1.0321241047696823 1107 72-73 1.3072387431865273 1107 74-75 0.8525145591390526 1107 76-77 1.243735031510031 1107 78-79 1.2291800666325017 1107 80-81 1.2140078799607856 1107 82-83 1.1550257590882218 1107 84-85 1.4396714015857484 1107 86-87 1.735042653856798 1107 88-89 1.5870947167879628 1107 90-91 1.5400692355254115 1107 92-93 1.4520340792096427 1107 94-95 1.5612571580891164 1107 96-97 1.7395134966989687 1107 98-99 1.8396481703012704 1107 100-101 1.8000543700745357 1108 1 0.3360258096433313 1108 2 0.2781004370741762 1108 3 -0.0015039579110371903 1108 4 -0.036329652419588854 1108 5 0.19551989793176006 1108 6 0.19899320186454617 1108 7 0.32317098315177617 1108 8 0.3187233430889975 1108 9 0.2891809249873134 1108 10-11 0.39354127185487187 1108 12-13 0.27398242985165666 1108 14-15 0.4919785306493125 1108 16-17 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2316 100-101 -2.599125659670541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6010.0 3 1084.0 4 844.0 5 835.0 6 793.0 7 924.0 8 1168.0 9 1463.0 10 1681.0 11 2187.0 12 2988.0 13 3554.0 14 4274.0 15 5050.0 16 6072.0 17 7037.0 18 6562.0 19 5969.0 20 6423.0 21 6528.0 22 7026.0 23 7870.0 24 8686.0 25 9519.0 26 10967.0 27 12576.0 28 14595.0 29 16984.0 30 19481.0 31 22848.0 32 26801.0 33 32181.0 34 40072.0 35 49043.0 36 56893.0 37 52782.0 38 21140.0 39 862.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25508772221161 18.70230909106006 15.04894675224391 21.993656434484418 2 16.56613175026337 21.41133983987832 36.5699134977216 25.452614912136706 3 21.058329921801455 23.079480726749804 30.90099769124147 24.96119166020727 4 14.185277088502895 16.916052553919307 35.53894368451023 33.35972667306757 5 13.943948938647374 36.885174327948604 33.85122351816046 15.319653215243559 6 32.201717059224414 34.93147554063263 15.92248300393943 16.94432439620352 7 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29.241722057172765 21.304429544418497 30-31 21.93248204558319 26.373129910526504 28.724262739457135 22.97012530443317 32-33 21.6272148796717 26.54366625408086 29.18677676338896 22.642342102858475 34-35 23.39769053501532 26.345950335806055 28.364733042754906 21.891626086423717 36-37 22.874401226984947 25.698044985986662 29.569340329445875 21.858213457582522 38-39 22.099567391694794 26.52126653426451 28.896199961172186 22.482966112868514 40-41 22.246516746610187 26.72584563632966 29.021885836499926 22.005751780560228 42-43 21.928372024455296 26.767418272120697 28.062751563234833 23.241458140189177 44-45 22.222833431242098 25.84500800222526 28.87464425905206 23.057514307480574 46-47 23.347179924726028 26.831870826041555 28.090104565638445 21.73084468359397 48-49 22.37747140800586 26.675482034191035 28.036709017103817 22.910337540699285 50-51 21.395988479202884 27.10667116427515 28.99348711694647 22.503853239575495 52-53 22.638009810617305 26.99684258380854 27.581722276079823 22.783425329494335 54-55 21.4954153770144 26.347414156385778 28.287653175398937 23.869517291200886 56-57 22.45177790902495 26.211352003877902 27.789501218072942 23.5473688690242 58-59 23.175816863877994 25.68560303690563 27.484016695922246 23.654563403294134 60-61 21.618580277607933 26.140012311831917 29.121634878402492 23.119772532157654 62-63 21.53863080354687 26.387500635742096 28.780693368872612 23.29317519183842 64-65 21.20646294971981 27.177643931617368 27.887895825570517 23.727997293092297 66-67 21.77807578214036 26.035294142069198 27.632788967933852 24.553841107856588 68-69 21.926642357359274 25.687734387082646 28.053895665116972 24.33172759044111 70-71 22.57518972623361 25.914059985673205 27.2651391671771 24.245611120916085 72-73 22.234253051597378 25.41281432127782 28.343988057296738 24.008944569828063 74-75 21.847219785456158 27.358544432251964 27.346605720407823 23.447630061884052 76-77 22.378199491963706 25.634344665359375 27.731162223857776 24.256293618819143 78-79 22.516671077691115 26.46199513230479 27.610003165558723 23.411330624445377 80-81 23.45844434457613 26.007196745999707 27.058893129256006 23.475465780168157 82-83 22.718766638885455 25.92183966263995 27.818400155270318 23.540993543204277 84-85 22.643099565122625 26.59446387611703 27.431317398208595 23.331119160551744 86-87 23.219636853561244 26.61205526601779 26.649837608585308 23.51847027183565 88-89 22.93156112238181 26.432307618832063 25.90799509118885 24.728136167597278 90-91 23.157320985475348 25.90869921822278 26.364268732025874 24.569711064276 92-93 24.11043065797246 25.856711726068184 26.65038147017328 23.382476145786075 94-95 23.489667374821664 25.454018727688442 26.844577630193566 24.21173626729632 96-97 23.247650087579856 25.13173318824945 27.22513578252369 24.395480941646998 98-99 23.636148463120392 25.95654651852052 25.7451859941471 24.662119024211993 100-101 24.32784167745125 24.567976119502198 26.182340147528965 24.921842055517587 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1023.0 1 641.5 2 303.5 3 350.5 4 300.0 5 298.5 6 432.5 7 756.0 8 1071.0 9 1131.5 10 949.5 11 706.0 12 627.5 13 627.5 14 695.5 15 715.5 16 715.5 17 783.0 18 853.0 19 974.5 20 1104.0 21 1272.5 22 1515.0 23 1791.5 24 2065.0 25 2510.0 26 3002.0 27 3531.0 28 3991.5 29 4378.5 30 4768.5 31 5646.5 32 7234.5 33 7105.5 34 6154.0 35 6203.5 36 6386.5 37 6869.0 38 8054.0 39 9106.0 40 10154.0 41 11451.5 42 12352.0 43 12811.0 44 13217.0 45 15114.5 46 17411.5 47 21004.5 48 26659.0 49 27012.0 50 22215.5 51 18820.0 52 17505.5 53 17542.0 54 17584.5 55 16638.0 56 15444.5 57 14379.0 58 13460.0 59 11802.5 60 9327.5 61 7523.5 62 6492.5 63 5013.0 64 3429.0 65 2493.0 66 1833.0 67 1536.0 68 1274.5 69 982.5 70 755.0 71 558.0 72 438.0 73 369.0 74 264.5 75 201.0 76 172.0 77 123.0 78 79.0 79 61.0 80 43.5 81 26.5 82 23.5 83 14.5 84 8.0 85 6.0 86 4.0 87 2.0 88 1.5 89 1.5 90 1.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004981609558048205 2 0.10648190430328039 3 0.11644512341937681 4 0.12246456830201838 5 0.16231744476640403 6 0.1004624594206388 7 0.025323181920078378 8 0.027398852569265133 9 0.020756706491867522 10-11 0.05417500394377424 12-13 0.05625067459296099 14-15 0.028540471426317843 16-17 0.03300316332206936 18-19 0.03829612347749558 20-21 0.06009066529395647 22-23 0.03186154446501665 24-25 0.04991987911294139 26-27 0.059052829969363105 28-29 0.05531662280082695 30-31 0.0715068538644836 32-33 0.05313716861918086 34-35 0.06611011017659806 36-37 0.05531662280082695 38-39 0.03289937978961002 40-41 0.03777720581519889 42-43 0.03289937978961002 44-45 0.006434579012478932 46-47 0.013491859219713888 48-49 0.007783764934450321 50-51 0.007161063739694295 52-53 0.010170786181015086 54-55 0.011001054440689786 56-57 0.014218343946929252 58-59 0.020237788829570835 60-61 0.025219398387619038 62-63 0.010689703843311773 64-65 0.008614033194125022 66-67 0.010170786181015086 68-69 0.013180508622335878 70-71 0.03258802919223201 72-73 0.028851822023695857 74-75 0.030201007945667244 76-77 0.02355886186826964 78-79 0.005085393090507543 80-81 0.005500527220344893 82-83 0.00653836254493827 84-85 0.005604310752804231 86-87 0.013595642752173228 88-89 0.039230175269629616 90-91 0.036739370490605515 92-93 0.03082370914042327 94-95 0.049608528515563376 96-97 0.04390043423029981 98-99 0.02708750197188712 100-101 0.07783764934450321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 481772.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.2159660663372 #Duplication Level Percentage of deduplicated Percentage of total 1 71.0758940501757 32.137652135073225 2 13.553438273773436 12.256636101382709 3 5.74136399662826 7.788039589381006 4 3.0906201129320676 5.589814966011025 5 1.8678900118766508 4.2229225696332415 6 1.1553898943406677 3.134524215353793 7 0.8213535230552601 2.59968051188532 8 0.5474676635703385 1.9803423438730667 9 0.3882946719997505 1.5801406841592223 >10 1.6070679649348318 11.900294506180382 >50 0.07560991835713589 2.4681891434126038 >100 0.058551583117070406 5.422928410132374 >500 0.010142793925791723 3.1081672405271656 >1k 0.006915541313039812 5.810667582994918 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4402 0.9137102197720083 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2970 0.6164741828084654 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2753 0.5714321297211129 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2465 0.5116528150245344 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1998 0.4147189957075131 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1761 0.3655256013217871 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1571 0.3260878589872388 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1358 0.28187607415956095 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1295 0.26879934906968445 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1262 0.26194963592736814 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1250 0.259458831148344 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1238 0.2569680263693199 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1224 0.2540620874604585 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1198 0.2486653437725729 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1129 0.23434321629318433 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 888 0.1843195536477836 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 861 0.17871524289497936 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 831 0.1724882309474191 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 821 0.17041256029823235 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 820 0.1702049932333137 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 776 0.16107204237689196 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 762 0.1581661034680305 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 762 0.1581661034680305 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 750 0.15567529868900642 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 746 0.1548450304293317 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 670 0.1390699334955124 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.1355412933918949 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 643 0.13346562274270818 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 637 0.1322202203531961 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 610 0.12661590960039187 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 569 0.11810565993872621 No Hit GTACAAAGTCCTCAGCCACATTTGTAGAACTTTGGGGGATGTTTGCTCCA 566 0.11748295874397018 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 514 0.10668947136819905 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 513 0.10648190430328039 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 509 0.10565163604360568 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 505 0.104821367783931 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 504 0.10461380071901231 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 493 0.1023305630049069 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.0756706491867523E-4 0.0 3 0.0 0.0 0.0 2.0756706491867523E-4 0.0 4 0.0 0.0 0.0 2.0756706491867523E-4 0.0 5 0.0 0.0 0.0 2.0756706491867523E-4 0.0 6 0.0 0.0 0.0 2.0756706491867523E-4 0.0 7 0.0 0.0 0.0 2.0756706491867523E-4 0.0 8 0.0 0.0 0.0 2.0756706491867523E-4 0.0 9 0.0 0.0 0.0 2.0756706491867523E-4 0.0 10-11 0.0 0.0 0.0 3.1135059737801284E-4 0.0 12-13 0.0 0.0 0.0 6.227011947560257E-4 0.0 14-15 0.0 0.0 0.0 6.227011947560257E-4 0.0 16-17 0.0 0.0 0.0 7.264847272153633E-4 0.0 18-19 0.0 0.0 0.0 9.340517921340385E-4 0.0 20-21 0.0 0.0 0.0 0.001349185921971389 0.0 22-23 0.0 0.0 0.0 0.0038399907009954914 0.0 24-25 0.0 0.0 0.0 0.011623755635445814 0.0 26-27 0.0 0.0 0.0 0.0209642735567862 0.0 28-29 0.0 0.0 0.0 0.05282581802180285 0.0 30-31 0.0 0.0 0.0 0.11831322700364487 0.0 32-33 0.0 0.0 0.0 0.2376642893318831 0.0 34-35 0.0 0.0 0.0 0.41824763581113056 0.0 36-37 0.0 0.0 0.0 0.6738664762584792 0.0 38-39 0.0 0.0 0.0 0.9593749740541169 0.0 40-41 0.0 0.0 0.0 1.2273440548641266 0.0 42-43 0.0 0.0 0.0 1.4995682605049692 0.0 44-45 0.0 0.0 0.0 1.8447522894647261 0.0 46-47 0.0 0.0 0.0 2.264349111197828 0.0 48-49 0.0 0.0 0.0 2.6204304110658154 0.0 50-51 0.0 0.0 0.0 2.9710111837134576 0.0 52-53 0.0 0.0 0.0 3.3089303654010607 0.0 54-55 0.0 0.0 0.0 3.700713200435061 0.0 56-57 0.0 0.0 0.0 4.186004998214923 0.0 58-59 0.0 0.0 0.0 4.6516817083599715 0.0 60-61 0.0 0.0 0.0 5.115075180790914 0.0 62-63 0.0 0.0 0.0 5.578780003819234 0.0 64-65 0.0 0.0 0.0 6.0865928281427735 0.0 66-67 0.0 0.0 0.0 6.70586916632764 0.0 68-69 0.0 0.0 0.0 7.368423237548052 0.0 70-71 0.0 0.0 0.0 8.036685403053728 0.0 72-73 0.0 0.0 0.0 8.697786504819707 0.0 74-75 0.0 0.0 0.0 9.393239955829728 0.0 76-77 0.0 0.0 0.0 10.10446850377357 0.0 78-79 0.0 0.0 0.0 10.956012387602435 0.0 80-81 0.0 0.0 0.0 11.79645143345815 0.0 82-83 0.0 0.0 0.0 12.6317013026909 0.0 84-85 0.0 0.0 0.0 13.501822438829986 0.0 86-87 0.0 0.0 0.0 14.369764120787426 0.0 88-89 0.0 0.0 0.0 15.286484063000756 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGACG 35 2.7570958E-4 54.40168 5 AGGTAAG 315 0.0 39.2901 2 AAGGTAA 295 0.0 38.71453 1 TAAGGTG 330 0.0 34.619247 5 GTAAGGT 360 0.0 34.378838 4 GGTAAGG 375 0.0 31.734312 3 GGTGTGC 410 0.0 31.291948 8 AGGTGTG 415 0.0 29.769936 7 TATTCTA 65 0.0057801465 29.293211 5 ACCGTGC 65 0.0058302986 29.241476 8 AAGGTGT 450 0.0 28.522293 6 GTGTGCA 470 0.0 27.291561 9 CCTAGAT 70 0.0082999505 27.20084 3 AGAGCGC 90 8.9208665E-4 26.398554 8 GTATATG 100 0.0016348239 23.793304 1 CTAGGAC 165 2.380446E-6 23.079498 3 TAAACCC 105 0.00216733 22.667366 4 GGTCATA 105 0.0021712664 22.660292 1 CTACTGG 150 2.9861787E-5 22.207087 1 GTAAGCG 90 5.1579045E-6 21.115555 94-95 >>END_MODULE