##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938406_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 478041 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.981974349480485 31.0 31.0 34.0 28.0 34.0 2 31.05598264584 31.0 31.0 34.0 27.0 34.0 3 31.106241933223302 31.0 31.0 34.0 27.0 34.0 4 34.70521356954738 37.0 35.0 37.0 32.0 37.0 5 34.729288910365426 37.0 35.0 37.0 32.0 37.0 6 34.78449128840413 37.0 35.0 37.0 32.0 37.0 7 34.72868226783895 37.0 35.0 37.0 32.0 37.0 8 34.699496486703026 37.0 35.0 37.0 32.0 37.0 9 36.203365401712404 39.0 37.0 39.0 32.0 39.0 10-11 36.081466861629025 39.0 35.0 39.0 32.0 39.0 12-13 35.975373451231164 39.0 35.0 39.0 31.0 39.0 14-15 36.94905248712976 39.5 36.0 41.0 31.0 41.0 16-17 36.83119230358902 39.0 36.0 41.0 31.0 41.0 18-19 36.84318081503469 39.0 36.0 41.0 31.0 41.0 20-21 36.89162749638629 39.0 36.0 41.0 31.0 41.0 22-23 36.84369959898837 39.0 36.0 41.0 31.0 41.0 24-25 36.69509623651528 39.0 36.0 41.0 30.5 41.0 26-27 36.470374926000076 39.0 36.0 41.0 30.0 41.0 28-29 36.288268161099154 39.0 35.5 41.0 30.0 41.0 30-31 36.07206808621018 39.0 35.0 40.5 29.5 41.0 32-33 35.730966590731754 38.0 35.0 40.0 28.5 41.0 34-35 35.45832261249558 38.0 35.0 40.0 27.0 41.0 36-37 35.12415148491448 38.0 34.5 40.0 25.5 41.0 38-39 34.83637805125502 38.0 34.0 40.0 25.0 41.0 40-41 34.74567453419267 38.0 34.0 40.0 24.5 41.0 42-43 34.51579048659006 38.0 34.0 40.0 24.0 41.0 44-45 34.20522089109512 38.0 33.0 40.0 23.0 41.0 46-47 33.78799830976841 38.0 33.0 40.0 20.5 41.0 48-49 33.35725910539054 38.0 33.0 40.0 17.0 41.0 50-51 32.53817768768788 36.5 32.0 39.5 14.0 40.5 52-53 32.554358308178585 37.0 32.0 39.5 12.5 40.5 54-55 32.656652881238216 37.0 32.0 40.0 8.5 41.0 56-57 32.292497923818246 36.5 31.5 40.0 6.5 41.0 58-59 31.956267349453288 36.0 31.0 39.5 2.0 41.0 60-61 31.466830250961735 35.5 31.0 39.0 2.0 41.0 62-63 30.97628969063323 35.0 30.0 39.0 2.0 40.0 64-65 30.486628761968113 35.0 29.5 38.0 2.0 40.0 66-67 29.930489225819542 35.0 29.0 38.0 2.0 40.0 68-69 29.385258795793668 34.0 28.0 37.0 2.0 39.0 70-71 28.751944916858598 34.0 26.5 36.0 2.0 39.0 72-73 28.254054568541193 34.0 26.0 36.0 2.0 39.0 74-75 27.733918220403687 34.0 26.0 35.0 2.0 37.5 76-77 27.09100579239019 33.0 25.0 35.0 2.0 37.0 78-79 26.366189301754453 32.5 23.5 35.0 2.0 36.5 80-81 25.764388410199125 32.0 20.5 35.0 2.0 36.0 82-83 25.169757405745532 32.0 19.0 35.0 2.0 35.5 84-85 24.5650571812878 31.0 16.0 34.0 2.0 35.0 86-87 24.002383686754904 31.0 8.5 34.0 2.0 35.0 88-89 23.254139289307822 30.5 2.0 34.0 2.0 35.0 90-91 22.484316198819766 30.0 2.0 34.0 2.0 35.0 92-93 21.7367621187304 29.0 2.0 34.0 2.0 35.0 94-95 20.825976223796705 29.0 2.0 34.0 2.0 35.0 96-97 19.693106867402587 27.0 2.0 34.0 2.0 35.0 98-99 18.321757966366903 25.0 2.0 33.0 2.0 35.0 100-101 14.953034363161319 9.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7896042714998224 1101 2 0.6941686677240106 1101 3 0.7209785936894519 1101 4 0.5236950720208 1101 5 0.7283832429315211 1101 6 0.8129814494133925 1101 7 0.6047633109022286 1101 8 0.6368811457736641 1101 9 0.6598613521358629 1101 10-11 0.840080743144128 1101 12-13 0.9057060587653325 1101 14-15 1.089516226909538 1101 16-17 0.950964762019332 1101 18-19 1.0432708051021393 1101 20-21 1.0770161259443114 1101 22-23 1.304111734900836 1101 24-25 1.3773348697757797 1101 26-27 1.402552927780114 1101 28-29 1.3397443478228297 1101 30-31 1.190028345788356 1101 32-33 0.9717778234173338 1101 34-35 0.8990087224709598 1101 36-37 0.7594897411573598 1101 38-39 0.8888760642386444 1101 40-41 0.5711384248286109 1101 42-43 0.8386282715251809 1101 44-45 0.9872968705385432 1101 46-47 0.7969835463421973 1101 48-49 0.6987094758145602 1101 50-51 0.460694386868461 1101 52-53 0.5787328467093573 1101 54-55 0.9789373153266183 1101 56-57 0.5813096235615802 1101 58-59 0.9528772589248149 1101 60-61 0.8931719413496424 1101 62-63 0.7794863611225473 1101 64-65 0.6413573993887915 1101 66-67 0.7117717287914402 1101 68-69 0.6946289793730926 1101 70-71 0.8343829200515742 1101 72-73 0.7488067631412392 1101 74-75 0.9977550935729589 1101 76-77 1.1816254534914066 1101 78-79 1.190430218381195 1101 80-81 0.951833409397242 1101 82-83 1.155158236621439 1101 84-85 1.546503242315822 1101 86-87 1.6685102362969282 1101 88-89 1.8373006621486248 1101 90-91 1.8674555161791737 1101 92-93 1.4970089976820695 1101 94-95 1.595211386054185 1101 96-97 1.3076723980823317 1101 98-99 1.2736510438607915 1101 100-101 1.600459578997219 1102 1 0.9503581792816327 1102 2 1.0222833522472605 1102 3 0.9432096405133414 1102 4 0.744839038226992 1102 5 0.7267253208935642 1102 6 0.8373092215930598 1102 7 0.839354315434214 1102 8 0.8750957523646505 1102 9 1.0428760897721858 1102 10-11 1.151740303248765 1102 12-13 1.0469272622975936 1102 14-15 1.1193613055119158 1102 16-17 1.2507979770478457 1102 18-19 1.3567769131053353 1102 20-21 1.3271638001378179 1102 22-23 1.4078435486377643 1102 24-25 1.3489218585624698 1102 26-27 1.1595755182088325 1102 28-29 1.0298494322825391 1102 30-31 1.1870301873724216 1102 32-33 1.321606819183181 1102 34-35 1.253012306362315 1102 36-37 1.4171338041584036 1102 38-39 1.223247667111714 1102 40-41 1.4502193205036207 1102 42-43 1.3765619248606242 1102 44-45 1.3171265612417642 1102 46-47 1.2991179968701019 1102 48-49 0.9881434493175973 1102 50-51 1.3420189375169826 1102 52-53 1.2261216194995797 1102 54-55 1.3158330300448995 1102 56-57 1.4055053576388303 1102 58-59 1.5413105225989554 1102 60-61 1.4268143563563171 1102 62-63 1.3156777071666177 1102 64-65 1.3298417905988273 1102 66-67 1.1011575530616327 1102 68-69 0.8750793980728702 1102 70-71 0.8937280904849665 1102 72-73 0.9969540104446359 1102 74-75 0.9306712990163 1102 76-77 1.2984593187827578 1102 78-79 1.0490054974730114 1102 80-81 1.0703698712724794 1102 82-83 1.544072496240112 1102 84-85 1.5359352302834814 1102 86-87 1.4069358060214832 1102 88-89 1.4484335589950987 1102 90-91 1.570426869867557 1102 92-93 1.7424211700066827 1102 94-95 1.6309187578506474 1102 96-97 1.8494703996409783 1102 98-99 1.5759339131579608 1102 100-101 1.247203052790633 1103 1 0.40026837917433866 1103 2 0.42170489960806634 1103 3 0.3278386611090518 1103 4 0.289512842252833 1103 5 0.37664485066754594 1103 6 0.13753547121403642 1103 7 0.33344616811538685 1103 8 0.05290929468732486 1103 9 0.3784952440184455 1103 10-11 0.16041242789597732 1103 12-13 0.12316871962505616 1103 14-15 0.34787109022490625 1103 16-17 0.12195464907919984 1103 18-19 0.23348019955601984 1103 20-21 0.185699475271349 1103 22-23 0.2260624743019335 1103 24-25 0.2810306449781663 1103 26-27 0.15861454043727718 1103 28-29 -0.09521296129850754 1103 30-31 0.1905032356778804 1103 32-33 0.14181300955940657 1103 34-35 0.10003724047002294 1103 36-37 0.23877912872164586 1103 38-39 0.3399574844792852 1103 40-41 0.29902159699464903 1103 42-43 0.22479741106406692 1103 44-45 0.15032927623721548 1103 46-47 0.01916557926461593 1103 48-49 -0.1039774659437569 1103 50-51 -0.3285406221126195 1103 52-53 -0.034126088848331904 1103 54-55 -0.2417929816641049 1103 56-57 -0.02382312415623744 1103 58-59 -0.3152682099366757 1103 60-61 -0.590317638139048 1103 62-63 -0.14603712501214616 1103 64-65 -0.4028221237528662 1103 66-67 -0.2043400794809962 1103 68-69 0.11158722351078865 1103 70-71 0.1021273161048235 1103 72-73 -0.07313905512110352 1103 74-75 -0.17212999783719596 1103 76-77 0.04865874499505907 1103 78-79 -0.11508145702049077 1103 80-81 0.0953879479174553 1103 82-83 0.19584867238827286 1103 84-85 -0.0406304404183615 1103 86-87 0.06345622679912921 1103 88-89 0.171037528859582 1103 90-91 0.017272203760679616 1103 92-93 -0.009462299776163974 1103 94-95 0.37611736769833826 1103 96-97 0.32035417264496857 1103 98-99 0.5716678249439333 1103 100-101 1.2574635897252993 1104 1 0.5714565737125135 1104 2 0.5031553877531181 1104 3 0.5904337031750089 1104 4 0.6792775428422075 1104 5 0.568573585989455 1104 6 0.6047195266421213 1104 7 0.6917581310433576 1104 8 0.23106649089014297 1104 9 0.6161341516533057 1104 10-11 0.36175763867566246 1104 12-13 0.5517330782879384 1104 14-15 0.937284866263056 1104 16-17 1.0149159124075808 1104 18-19 0.775103000653786 1104 20-21 0.7700113482186666 1104 22-23 0.8809191801101832 1104 24-25 0.8775933839892716 1104 26-27 0.7434685468259019 1104 28-29 0.8009696094487921 1104 30-31 0.7440147307064464 1104 32-33 0.6846977893317572 1104 34-35 0.49633453655136606 1104 36-37 0.8601810273134518 1104 38-39 0.6898225134980507 1104 40-41 0.7599902387366768 1104 42-43 1.1481631374328956 1104 44-45 1.0603103802998461 1104 46-47 1.3215950569269168 1104 48-49 1.2733339121099618 1104 50-51 1.2205370761822465 1104 52-53 1.3917815910005018 1104 54-55 1.4808259052752106 1104 56-57 1.5745212318072248 1104 58-59 1.19991152642001 1104 60-61 1.2411823393657926 1104 62-63 1.0749058722434448 1104 64-65 1.0483521237839177 1104 66-67 1.4322460545001974 1104 68-69 1.410955109415319 1104 70-71 0.9219163114708202 1104 72-73 0.9568987367536437 1104 74-75 1.0072171925168796 1104 76-77 1.0933476368833013 1104 78-79 1.0246292551763858 1104 80-81 0.9682610302047614 1104 82-83 1.0410864462668883 1104 84-85 1.3611174337886816 1104 86-87 1.0035853490378486 1104 88-89 1.0400571896325168 1104 90-91 1.2013433784581231 1104 92-93 1.1325159249055297 1104 94-95 0.8078512949039869 1104 96-97 0.777993530183096 1104 98-99 0.2939132921684795 1104 100-101 0.719802186926902 1105 1 0.1139978641771684 1105 2 0.13188033820456013 1105 3 0.22633328476496573 1105 4 0.20790559833658762 1105 5 0.214607499224428 1105 6 0.30808442424176974 1105 7 0.22700587383128834 1105 8 0.20749899474391498 1105 9 0.35807645511069097 1105 10-11 0.2372432145400012 1105 12-13 0.3707861502985139 1105 14-15 0.4154658836601044 1105 16-17 0.3835505743763079 1105 18-19 0.3295865945758294 1105 20-21 0.3624623502006088 1105 22-23 0.6455304992028985 1105 24-25 0.6905156478078212 1105 26-27 0.6447039575283924 1105 28-29 0.5508198626969758 1105 30-31 0.17629836867165238 1105 32-33 0.37452381884067876 1105 34-35 0.5451872121734809 1105 36-37 0.7860343635829921 1105 38-39 0.6762902518816674 1105 40-41 1.0579576467965808 1105 42-43 1.0018319328524115 1105 44-45 1.0008895478840785 1105 46-47 1.0620178712849864 1105 48-49 0.8256659975536991 1105 50-51 0.8228911832071333 1105 52-53 1.0175886819496327 1105 54-55 0.8720723267682686 1105 56-57 1.0513325068567738 1105 58-59 1.0121907600689894 1105 60-61 1.1760094699537618 1105 62-63 1.1394863611225468 1105 64-65 1.3600301682675102 1105 66-67 1.129163033139264 1105 68-69 0.9780614736522715 1105 70-71 1.2588222793193076 1105 72-73 1.1731088226378112 1105 74-75 1.0847573819024738 1105 76-77 0.9609847212259588 1105 78-79 1.354686511287376 1105 80-81 1.472931807566578 1105 82-83 1.1772634997793325 1105 84-85 1.5588602217208596 1105 86-87 1.606908405633309 1105 88-89 1.4716713715307783 1105 90-91 1.266723250732948 1105 92-93 1.5878099130138743 1105 94-95 1.594204521065624 1105 96-97 1.673393221880957 1105 98-99 2.1765801056456873 1105 100-101 1.7323588924983628 1106 1 0.35523728841626223 1106 2 0.21391088429839655 1106 3 0.33848359791736726 1106 4 0.4196405725152488 1106 5 0.4239207615032967 1106 6 0.1801556252086911 1106 7 0.40905619797149484 1106 8 0.26878017263645404 1106 9 0.6666403491882633 1106 10-11 0.10716080601346789 1106 12-13 0.2938693279712936 1106 14-15 0.5638429850775353 1106 16-17 0.30851266645410647 1106 18-19 0.1401832323079475 1106 20-21 0.37828258973472373 1106 22-23 0.4555953272437634 1106 24-25 0.5758271520381228 1106 26-27 0.20756957669112097 1106 28-29 0.48560505113780295 1106 30-31 0.5336217397297744 1106 32-33 0.4865994149290245 1106 34-35 0.3493581823965357 1106 36-37 0.2058453733621377 1106 38-39 0.43925036615482327 1106 40-41 0.34347352417269406 1106 42-43 0.18226704987252162 1106 44-45 0.545157210778271 1106 46-47 0.4623738761196208 1106 48-49 1.2021037516466535 1106 50-51 1.0958897547926725 1106 52-53 1.038087528230264 1106 54-55 0.9210889183516144 1106 56-57 0.28260379572613203 1106 58-59 -0.01013206159896285 1106 60-61 1.0007210401108892 1106 62-63 1.2209213682646158 1106 64-65 1.0587577806177997 1106 66-67 0.5432164338644618 1106 68-69 0.1566923933314257 1106 70-71 0.2086036220288996 1106 72-73 0.8342823200556779 1106 74-75 1.0713566567074402 1106 76-77 0.6805935554200033 1106 78-79 0.6437822150561914 1106 80-81 0.9562786925242754 1106 82-83 0.9069986937140655 1106 84-85 1.0354891635953756 1106 86-87 0.9356810679781091 1106 88-89 0.4735876444678162 1106 90-91 0.4535664547764604 1106 92-93 0.6723456783143895 1106 94-95 0.2375536035840291 1106 96-97 0.37937498212708576 1106 98-99 0.04320575117914771 1106 100-101 0.16415539837683646 1107 1 0.3905377286899352 1107 2 0.568899619080014 1107 3 0.34395704714120257 1107 4 0.4676242017758838 1107 5 0.37293996404985563 1107 6 0.31781866082332044 1107 7 0.4220866450663081 1107 8 0.36126658202896067 1107 9 0.4600879194150167 1107 10-11 0.5306668726952992 1107 12-13 0.30628380532716903 1107 14-15 0.33783169918902445 1107 16-17 0.2527115957416086 1107 18-19 0.3406131709176776 1107 20-21 0.4131241532230163 1107 22-23 0.523070884820406 1107 24-25 0.4087960021590433 1107 26-27 0.32607967404741345 1107 28-29 0.6264946933379321 1107 30-31 0.7588413598128696 1107 32-33 0.5401309058651691 1107 34-35 0.5817896092552957 1107 36-37 0.786868131170273 1107 38-39 0.6261599756961544 1107 40-41 0.2534344576355565 1107 42-43 0.5463134336340687 1107 44-45 0.9722392091659913 1107 46-47 0.7100949947868074 1107 48-49 0.3778494264229053 1107 50-51 0.3946681503775338 1107 52-53 0.376285920094503 1107 54-55 0.5955220401606596 1107 56-57 0.7005247945065918 1107 58-59 0.8365576178073866 1107 60-61 0.6948734616160834 1107 62-63 0.8262294564847146 1107 64-65 0.8188003610762635 1107 66-67 0.47418648285265874 1107 68-69 0.40030222404310223 1107 70-71 0.6708806430504204 1107 72-73 0.7196747423876815 1107 74-75 0.7438819259358276 1107 76-77 0.43562776447765117 1107 78-79 0.2939465425536625 1107 80-81 0.19109751887454962 1107 82-83 0.008102478414038217 1107 84-85 0.3049779913925583 1107 86-87 0.07050402835347924 1107 88-89 0.414016342870017 1107 90-91 0.6231276280127851 1107 92-93 0.641660877057646 1107 94-95 0.6372373506643747 1107 96-97 0.6377288133348387 1107 98-99 -0.09598894073263509 1107 100-101 0.016475619960582222 1108 1 0.29929391680874673 1108 2 0.38667885794140133 1108 3 0.30672390976496544 1108 4 0.40496563123132745 1108 5 0.5298706571191616 1108 6 0.41439199003124116 1108 7 0.4650321896207643 1108 8 0.20802942237549615 1108 9 0.13868089590016552 1108 10-11 0.37178885598737565 1108 12-13 0.4685904266142984 1108 14-15 0.44436596589694943 1108 16-17 0.3782298506921009 1108 18-19 0.6084949004968792 1108 20-21 0.4454125969111331 1108 22-23 0.5179811570976298 1108 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-0.627454693459164 2316 56-57 -0.5608140162387265 2316 58-59 -0.08209524174242944 2316 60-61 -0.6615773268662011 2316 62-63 -0.42248606516305287 2316 64-65 -0.7445849067048158 2316 66-67 -0.5028083868063931 2316 68-69 -0.3531612293768127 2316 70-71 -0.8982653728896075 2316 72-73 -0.34137442180216127 2316 74-75 -0.6342242020765916 2316 76-77 -0.7946336746681766 2316 78-79 -0.32653317272188076 2316 80-81 -0.4682194453266497 2316 82-83 -0.48331413934716494 2316 84-85 -0.4006748492261103 2316 86-87 -0.6315518942559919 2316 88-89 -0.7596580035321203 2316 90-91 -0.5937457036798506 2316 92-93 -0.9639843911020556 2316 94-95 -1.4718174171451004 2316 96-97 -1.5809410842476552 2316 98-99 -1.7032206390458704 2316 100-101 -1.161586966412779 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5784.0 3 1100.0 4 862.0 5 746.0 6 754.0 7 957.0 8 1114.0 9 1309.0 10 1621.0 11 2050.0 12 2710.0 13 3327.0 14 3956.0 15 5076.0 16 5710.0 17 6623.0 18 6140.0 19 5576.0 20 5720.0 21 6146.0 22 6519.0 23 6923.0 24 7834.0 25 9088.0 26 10298.0 27 11730.0 28 13492.0 29 15747.0 30 18328.0 31 21810.0 32 25897.0 33 31264.0 34 39198.0 35 49203.0 36 58852.0 37 57649.0 38 25614.0 39 1314.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.37900825753447 18.717567171060903 15.014251582344803 21.889172989059816 2 16.56195323683792 21.56705984780625 36.5147798715813 25.356207043774535 3 21.009870155963533 23.153217637357578 31.055411842512832 24.78150036416606 4 14.176909987839872 16.902369446054866 35.54064434030779 33.380076225797474 5 13.94567078454602 36.73396769048506 33.994273203364074 15.326088321604841 6 32.26402021263457 34.87035319778699 15.986737028516645 16.87888956106179 7 26.996304341457083 33.43378158365037 20.888213653428426 18.681700421464118 8 26.53503677893922 31.24880978539515 20.001255611011707 22.214897824653924 9 25.203000217649716 17.423069195868006 21.547112792780727 35.82681779370155 10-11 25.205355890914948 25.461200555010265 26.69312662061114 22.640316933463644 12-13 27.16196519175091 23.84510507035209 26.62664684161127 22.366282896285732 14-15 23.2423309609658 26.00023437679837 26.482485565946725 24.274949096289102 16-17 21.728335643849235 28.376029923360623 27.53179357066255 22.363840862127592 18-19 21.56096642216618 27.484226056566456 29.61954190166267 21.335265619604684 20-21 22.61817930667861 25.77294127372978 29.165668169731575 22.44321124986004 22-23 21.885810795255964 25.93199126869685 29.866937618899126 22.315260317148052 24-25 23.16501206282087 26.16852362585892 29.226985132115363 21.43947917920485 26-27 22.065051659779044 25.497630232560574 30.62991128938581 21.807406818274575 28-29 21.42684487490857 28.06361251531662 29.25545774076791 21.2540848690069 30-31 21.885961974767053 26.329983172453055 28.712315922543052 23.07173893023684 32-33 21.664981864834942 26.590881978061997 29.115887158042785 22.628248999060276 34-35 23.426417558406985 26.238617361257905 28.376805891869378 21.958159188465732 36-37 22.817845251026018 25.672691960763384 29.566739706876454 21.942723081334144 38-39 22.0621514144296 26.458135206475646 28.958169208444623 22.521544170650127 40-41 22.384102911760028 26.684682083343798 28.954289803399703 21.97692520149647 42-43 21.94227105675288 26.777600977527538 28.03319660083211 23.246931364887473 44-45 22.2065671716728 25.753429815863132 28.9439221445397 23.09608086792437 46-47 23.37275692458329 26.888346782545387 28.030257065805248 21.708639227066076 48-49 22.30370030200356 26.74379072523743 28.03454996030123 22.91795901245778 50-51 21.435385677524636 27.041465302621393 28.998410008577586 22.524739011276388 52-53 22.67533633131341 26.96382862767619 27.56708581901029 22.793749222000113 54-55 21.421652415691902 26.414758807395305 28.398004933271125 23.765583843641664 56-57 22.476317595273564 26.165814490619088 27.86134495974828 23.49652295435907 58-59 23.139831062527787 25.658202358848296 27.636810586050892 23.56515599257303 60-61 21.5565937077655 26.206834509797357 29.205771717163266 23.03080006527388 62-63 21.497770999776158 26.444352397508876 28.871881125566663 23.185995477148303 64-65 21.221668528583564 27.15940519077326 27.99402512960197 23.624901151041207 66-67 21.72377255549245 26.08461441103897 27.638329719433784 24.553283314034797 68-69 21.95998071107513 25.685441987303204 28.105135100926894 24.24944220069478 70-71 22.620382730669924 26.02085225156612 27.2969698745015 24.06179514326246 72-73 22.266217945163778 25.495631869827523 28.370197332150504 23.867952852858192 74-75 21.89383715699505 27.356181150550796 27.43861741413761 23.311364278316542 76-77 22.33215266779503 25.7853378495708 27.701740034604033 24.180769448030144 78-79 22.495261645056424 26.64168831875217 27.576388976055092 23.286661060136314 80-81 23.454510840913464 26.023317796503797 27.22571359235488 23.296457770227864 82-83 22.716194532716045 26.026187901014 27.902520255979514 23.355097310290436 84-85 22.58826408988068 26.561076459457567 27.592118599657123 23.258540851004632 86-87 23.18665938631671 26.67160391548936 26.689909393115883 23.451827305078044 88-89 22.851675502169382 26.503474042245106 26.089058100884806 24.555792354700706 90-91 23.19791659037333 25.983817791640202 26.395540214346774 24.42272540363969 92-93 24.103612957896775 25.900728659381816 26.715174213931885 23.280484168789524 94-95 23.466888473872263 25.402574846853632 27.017896284206177 24.112640395067928 96-97 23.237754445438423 25.09042589366522 27.44667828052168 24.225141380374676 98-99 23.568550854878346 25.951727593901662 25.892409718088395 24.587311833131597 100-101 24.223853556879707 24.71772208899268 26.21912012650407 24.839304227623547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1145.0 1 726.5 2 340.0 3 365.5 4 307.0 5 295.5 6 441.5 7 778.0 8 1077.0 9 1136.5 10 947.5 11 706.0 12 652.0 13 643.0 14 678.0 15 735.0 16 736.5 17 783.0 18 895.5 19 1011.5 20 1149.0 21 1341.5 22 1541.5 23 1790.0 24 2088.5 25 2495.5 26 2923.5 27 3419.5 28 3979.5 29 4409.5 30 4717.0 31 5476.5 32 7073.0 33 7098.5 34 6079.0 35 6044.0 36 6289.0 37 6841.0 38 8001.5 39 9125.5 40 10074.5 41 11118.5 42 12016.0 43 12610.5 44 13246.0 45 15038.0 46 17289.5 47 21116.0 48 26911.5 49 27017.0 50 21774.0 51 18666.0 52 17468.5 53 17181.0 54 17249.0 55 16474.5 56 15340.0 57 14266.0 58 13419.0 59 11777.5 60 9187.0 61 7417.0 62 6443.0 63 4935.5 64 3312.0 65 2428.5 66 1889.5 67 1550.5 68 1234.0 69 925.5 70 712.0 71 532.5 72 416.0 73 333.5 74 248.5 75 207.0 76 167.0 77 113.5 78 74.5 79 48.0 80 25.0 81 19.5 82 21.5 83 14.5 84 6.5 85 2.5 86 3.5 87 2.0 88 1.0 89 2.5 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18847755736432648 2 0.04790384088394092 3 0.049577337508707416 4 0.052296769523952964 5 0.060036691413498 6 0.06693986499065979 7 0.03828123529153357 8 0.03890879652582101 9 0.043720099322024676 10-11 0.04434766055631212 12-13 0.04068688668963541 14-15 0.037235299901054515 16-17 0.03211021648770712 18-19 0.04100066730677913 20-21 0.05010030520394694 22-23 0.04602115718107861 24-25 0.06976389054495326 26-27 0.05261055014109668 28-29 0.0440338799391684 30-31 0.0529243307582404 32-33 0.05020489874299485 34-35 0.04811302796203673 36-37 0.04643953133727023 38-39 0.026671352457216014 40-41 0.02447488813720999 42-43 0.021023301348629092 44-45 0.00564805110858692 46-47 0.014643095466706831 48-49 0.013910940693371489 50-51 0.010668540982886405 52-53 0.010982321600030123 54-55 0.012969598841940336 56-57 0.010250166826694782 58-59 0.011191508678125935 60-61 0.011505289295269653 62-63 0.005020489874299485 64-65 0.008158296045736663 66-67 0.0053342704914432024 68-69 0.009727199131455252 70-71 0.02238301735625187 72-73 0.018931430567670975 74-75 0.02008195949719794 76-77 0.012969598841940336 78-79 0.00564805110858692 80-81 0.007739921889545039 82-83 0.007112360655257603 84-85 0.00617101880382645 86-87 0.008785857280024099 88-89 0.029181597394365755 90-91 0.03566639681533593 92-93 0.022696797973395588 94-95 0.03106428109722806 96-97 0.03420208726866524 98-99 0.022487610895299777 100-101 0.12258362776414575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 478041.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.37732624070864 #Duplication Level Percentage of deduplicated Percentage of total 1 70.92750597477131 32.18500578057009 2 13.551043787390354 12.298202696850801 3 5.957193243437656 8.109645038592472 4 3.184136415192845 5.779503876285049 5 1.8385312354501637 4.171381583737759 6 1.1516843354425124 3.1356213489413163 7 0.7944258028822285 2.5234243181996643 8 0.5408959822361105 1.9635530758573223 9 0.3512405443633139 1.4344521093484373 >10 1.5442318096895848 11.436557074337122 >50 0.08418075053627072 2.68316308291957 >100 0.05920404433195975 5.718932077773096 >500 0.008788100330525274 2.754943572109867 >1k 0.006937973945151532 5.805614364477451 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4536 0.9488725862426026 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2897 0.606014965243567 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2833 0.592626992245435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2397 0.501421426195661 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1978 0.41377204047351585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.36001096140289224 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1616 0.338046318202832 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1311 0.27424425938360936 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1310 0.27403507230551355 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1262 0.26399409255691453 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1245 0.26043791222928575 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1187 0.24830506169972866 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1142 0.23889164318541714 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1116 0.23345277915492604 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1110 0.23219765668635117 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 868 0.1815743837871647 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 844 0.17655389391286522 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 830 0.17362527481952383 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 795 0.16630372708617044 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 754 0.15772705688424216 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 738 0.15438006363470916 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 734 0.1535433153223259 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 723 0.15124225746327197 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.15061469622898455 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 713 0.14915038668231387 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 692 0.1447574580423018 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 676 0.14141046479276884 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 652 0.13638997491846935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.13408891705941542 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 566 0.11839988620222952 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 551 0.11526208003079234 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 549 0.11484370587460072 No Hit GTACAAAGTCCTCAGCCACATTTGTAGAACTTTGGGGGATGTTTGCTCCA 542 0.11337939632793002 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 538 0.11254264801554678 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 499 0.10438435196981012 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 489 0.10229248118885201 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 489 0.10229248118885201 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 479 0.10020061040789388 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.0459353904790593E-4 0.0 20-21 0.0 0.0 0.0 0.0011505289295269652 0.0 22-23 0.0 0.0 0.0 0.0039745544838204255 0.0 24-25 0.0 0.0 0.0 0.010354760365742687 0.0 26-27 0.0 0.0 0.0 0.02029114657529375 0.0 28-29 0.0 0.0 0.0 0.05093705351633019 0.0 30-31 0.0 0.0 0.0 0.1197596022098523 0.0 32-33 0.0 0.0 0.0 0.2525933968006928 0.0 34-35 0.0 0.0 0.0 0.4489154695936122 0.0 36-37 0.0 0.0 0.0 0.7060063885733651 0.0 38-39 0.0 0.0 0.0 1.0100598065856277 0.0 40-41 0.0 0.0 0.0 1.2845132530473329 0.0 42-43 0.0 0.0 0.0 1.5619999121414272 0.0 44-45 0.0 0.0 0.0 1.920232783380505 0.0 46-47 0.0 0.0 0.0 2.360676176311237 0.0 48-49 0.0 0.0 0.0 2.735016452563692 0.0 50-51 0.0 0.0 0.0 3.113540470378064 0.0 52-53 0.0 0.0 0.0 3.4488673565656502 0.0 54-55 0.0 0.0 0.0 3.8501927658924653 0.0 56-57 0.0 0.0 0.0 4.344292644354773 0.0 58-59 0.0 0.0 0.0 4.7996929133693556 0.0 60-61 0.0 0.0 0.0 5.266912252296351 0.0 62-63 0.0 0.0 0.0 5.725345733943323 0.0 64-65 0.0 0.0 0.0 6.231892243552331 0.0 66-67 0.0 0.0 0.0 6.830794848140641 0.0 68-69 0.0 0.0 0.0 7.506992078085352 0.0 70-71 0.0 0.0 0.0 8.157563890963328 0.0 72-73 0.0 0.0 0.0 8.821628270378483 0.0 74-75 0.0 0.0 0.0 9.512677782867996 0.0 76-77 0.0 0.0 0.0 10.233641047525213 0.0 78-79 0.0 0.0 0.0 11.108043033965705 0.0 80-81 0.0 0.0 0.0 11.964768712307103 0.0 82-83 0.0 0.0 0.0 12.822644919578028 0.0 84-85 0.0 0.0 0.0 13.713781872266186 0.0 86-87 0.0 0.0 0.0 14.582431214059046 0.0 88-89 0.0 0.0 0.0 15.505992163852055 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACGCG 25 0.0016086386 38.033794 94-95 GTAAGCG 65 8.212737E-9 32.913864 94-95 TATACTA 60 0.0039405944 31.679897 5 AGGTAAG 220 7.6397555E-11 25.925344 2 TAAGGTG 220 7.6397555E-11 25.919916 5 TAATAGT 210 1.2514647E-9 24.89656 4 TAAGGTA 120 1.7015258E-4 23.764898 4 TAGGGAG 100 0.0016482238 23.75992 5 TATAGAG 105 0.002186372 22.633236 2 GTAAGGT 285 8.185452E-11 21.68026 3 GAGTACT 2505 0.0 20.95307 12-13 ATATGAC 115 0.0037066452 20.665129 3 TATGCTT 140 4.8323974E-4 20.365646 5 GTACTTT 2525 0.0 20.225925 14-15 TGTGCAC 365 5.456968E-12 19.532793 3 TAGTCAT 195 1.0305883E-5 19.487158 7 AGTACTT 2465 0.0 19.462454 12-13 TTAGACA 125 0.006004306 19.011919 4 ATAGTGC 125 0.006018853 19.003958 6 AAGGTAA 280 3.388959E-8 18.701801 1 >>END_MODULE